AlfalfaGEDB | MS.gene90379

M. sativa cultivar XinJiangDaYe / MS.gene90379

KEGG: K10839
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019538 GO:0019941 GO:0030163 GO:0033554 GO:0034641 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0046483 GO:0050896 GO:0051603 GO:0051716 GO:0071704 GO:0090304 GO:0097159 GO:0110165 GO:1901360 GO:1901363 GO:1901564 GO:1901565 GO:1901575
PFAM: PF00627.34 PF09280.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90379.t1	XP_013465631.1	100	101	0	0	1	101	266	366	6.00E-50	206.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90379.t1	Q84L33	79.6	103	19	1	1	101	269	371	2.8e-40	165.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90379.t1	B7FFJ7	100.0	101	0	0	1	101	42	142	4.3e-50	206.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051018	MS.gene90379	0.803747	3.01E-49	-1.69E-46
MS.gene053538	MS.gene90379	0.8029	4.50E-49	-1.69E-46
MS.gene053540	MS.gene90379	0.823743	1.17E-53	-1.69E-46
MS.gene055580	MS.gene90379	-0.812574	3.96E-51	-1.69E-46
MS.gene059900	MS.gene90379	-0.84381	1.11E-58	-1.69E-46
MS.gene060429	MS.gene90379	0.815922	7.21E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90379.t1	MTR_2g105410	100.000	101	0	0	1	101	266	366	2.52e-69	211
MS.gene90379.t1	MTR_1g111090	59.804	102	40	1	1	101	295	396	7.18e-37	128
MS.gene90379.t1	MTR_7g407040	54.455	101	44	1	1	101	272	370	1.40e-28	105

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90379.t1	AT1G79650	79.612	103	19	1	1	101	269	371	6.44e-53	169
MS.gene90379.t1	AT1G79650	79.612	103	19	1	1	101	263	365	8.50e-53	169
MS.gene90379.t1	AT1G79650	79.612	103	19	1	1	101	293	395	1.68e-52	169
MS.gene90379.t1	AT1G79650	80.000	100	18	1	4	101	252	351	2.97e-51	164
MS.gene90379.t1	AT1G16190	72.816	103	26	1	1	101	243	345	1.28e-47	155
MS.gene90379.t1	AT1G16190	72.816	103	26	1	1	101	266	368	1.34e-47	155
MS.gene90379.t1	AT5G38470	62.376	101	37	1	1	101	278	377	2.21e-37	129
MS.gene90379.t1	AT3G02540	55.238	105	43	2	1	101	314	418	6.75e-31	112
MS.gene90379.t1	AT3G02540	55.238	105	43	2	1	101	315	419	6.83e-31	112
MS.gene90379.t1	AT5G16090	45.745	94	47	3	3	95	79	169	5.54e-19	77.0
MS.gene90379.t1	AT5G38470	69.444	36	11	0	1	36	278	313	8.66e-11	57.0

Find 27 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTATTGCAGCCTGTACTTC+AGG	0.312035	2.1:+604675	None:intergenic
CCGATTGCCTCCTGCTCAGC+TGG	0.353647	2.1:-605315	None:intergenic
ATTGGCAGCCAACTACTTAC+TGG	0.376662	2.1:+605543	MS.gene90379:CDS
TGACATGCAGCTAGAGGCTA+TGG	0.454971	2.1:+605465	MS.gene90379:intron
TAATGTGACGCCAGCTGAGC+AGG	0.461283	2.1:+605305	MS.gene90379:CDS
CCAGCTGAGCAGGAGGCAAT+CGG	0.461594	2.1:+605315	MS.gene90379:CDS
AACTACTTACTGGAAAATGC+TGG	0.492726	2.1:+605553	MS.gene90379:CDS
TTTCCGAGCTCCTGAAGTAC+AGG	0.521041	2.1:-604685	None:intergenic
ACTACTTACTGGAAAATGCT+GGG	0.533868	2.1:+605554	MS.gene90379:CDS
TCACCCTTCAGTACCGTCCA+TGG	0.546504	2.1:-604776	None:intergenic
GAACCCATGGACGGTACTGA+AGG	0.549758	2.1:+604772	MS.gene90379:CDS
ATGTGACCGTGATGAGCAAT+TGG	0.553039	2.1:+605525	MS.gene90379:CDS
GAGCTCGGAAAGCAAAATCC+AGG	0.561167	2.1:+604697	MS.gene90379:CDS
CACCCTTCAGTACCGTCCAT+GGG	0.562359	2.1:-604775	None:intergenic
TCAGTTGATAAATGAACCCA+TGG	0.600530	2.1:+604759	MS.gene90379:CDS
CTGAGCAGGAGGCAATCGGA+AGG	0.602554	2.1:+605319	MS.gene90379:CDS
TCAATAAGTCTCAAGAGACC+TGG	0.604931	2.1:-604715	None:intergenic
CAGCCTGTACTTCAGGAGCT+CGG	0.605573	2.1:+604682	None:intergenic
TGATAGAGCCTCTGTCATAG+AGG	0.619305	2.1:+605492	MS.gene90379:CDS
GACATGCAGCTAGAGGCTAT+GGG	0.619380	2.1:+605466	MS.gene90379:intron
AACCCATGGACGGTACTGAA+GGG	0.627966	2.1:+604773	MS.gene90379:CDS
TGTGACGCCAGCTGAGCAGG+AGG	0.630237	2.1:+605308	MS.gene90379:CDS
CTACTTACTGGAAAATGCTG+GGG	0.630947	2.1:+605555	MS.gene90379:CDS
GGCGTCACATTAACAGCATG+AGG	0.631358	2.1:-605294	None:intergenic
TTGATAAATGAACCCATGGA+CGG	0.638539	2.1:+604763	MS.gene90379:CDS
TGGCTGCCAATTGCTCATCA+CGG	0.645634	2.1:-605531	None:intergenic
TGAGCAGGAGGCAATCGGAA+GGG	0.655930	2.1:+605320	MS.gene90379:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTATAAAAATAAATGGAG+AGG	+	chr2.1:605085-605104	MS.gene90379:intron	15.0%
!!	TCTCCATTTATAAAAATAAA+TGG	+	chr2.1:605080-605099	MS.gene90379:intron	15.0%
!!!	TCTCCATTTATTTTTATAAA+TGG	-	chr2.1:605086-605105	None:intergenic	15.0%
!!	TATAATAGAAACATGACTAT+GGG	-	chr2.1:604874-604893	None:intergenic	20.0%
!!	TGAAACTAATTAATGATGTT+GGG	+	chr2.1:604998-605017	MS.gene90379:intron	20.0%
!!	TTATAATAGAAACATGACTA+TGG	-	chr2.1:604875-604894	None:intergenic	20.0%
!!	TTGAAACTAATTAATGATGT+TGG	+	chr2.1:604997-605016	MS.gene90379:intron	20.0%
!!!	TATTAGAGATAATTTTGATC+AGG	+	chr2.1:605257-605276	MS.gene90379:intron	20.0%
!!!	TCAATTTCATTTTTTTGCAA+AGG	-	chr2.1:605414-605433	None:intergenic	20.0%
!	ATACACACAAAATATAGATG+TGG	-	chr2.1:605043-605062	None:intergenic	25.0%
!	ATGATTGTAAAATGCAAAGA+TGG	-	chr2.1:604843-604862	None:intergenic	25.0%
!	TAAAAATGAATCAGCACATA+TGG	-	chr2.1:605441-605460	None:intergenic	25.0%
!	TACACACAAAATATAGATGT+GGG	-	chr2.1:605042-605061	None:intergenic	25.0%
	TGTGTGTATCTCTTTCTAAA+TGG	+	chr2.1:605054-605073	MS.gene90379:intron	30.0%
	TGTTTCTTTATCTCTCCAAA+TGG	-	chr2.1:604970-604989	None:intergenic	30.0%
!!	TAGAGATAATTTTGATCAGG+CGG	+	chr2.1:605260-605279	MS.gene90379:intron	30.0%
!!!	CATTTTTTTGCAAAGGCATA+AGG	-	chr2.1:605407-605426	None:intergenic	30.0%
	AAACTATCACAGACCATACA+TGG	-	chr2.1:604814-604833	None:intergenic	35.0%
	AACTACTTACTGGAAAATGC+TGG	+	chr2.1:605553-605572	MS.gene90379:CDS	35.0%
	ACTACTTACTGGAAAATGCT+GGG	+	chr2.1:605554-605573	MS.gene90379:CDS	35.0%
	AGTATACTGCTTTCCATGTA+TGG	+	chr2.1:604798-604817	MS.gene90379:intron	35.0%
	GTAGGAATGGAGATCTAAAA+TGG	-	chr2.1:604948-604967	None:intergenic	35.0%
	TCTTTATCTCTCCAAATGGT+AGG	-	chr2.1:604966-604985	None:intergenic	35.0%
	TTACCTTCATGTCACAAACA+TGG	-	chr2.1:605128-605147	None:intergenic	35.0%
	TTGATAAATGAACCCATGGA+CGG	+	chr2.1:604763-604782	MS.gene90379:CDS	35.0%
!	AATGGAGATCTAAAATGGAG+AGG	-	chr2.1:604943-604962	None:intergenic	35.0%
!	AGCATTTTCCAGTAAGTAGT+TGG	-	chr2.1:605554-605573	None:intergenic	35.0%
!!	TCAGTTGATAAATGAACCCA+TGG	+	chr2.1:604759-604778	MS.gene90379:CDS	35.0%
	AAACATGGCAAAGACGAGTT+AGG	-	chr2.1:605113-605132	None:intergenic	40.0%
	AAATGGAGAGGATCTTGACT+CGG	-	chr2.1:604931-604950	None:intergenic	40.0%
	AGATCTCCATTCCTACCATT+TGG	+	chr2.1:604952-604971	MS.gene90379:intron	40.0%
	ATCTCTCCAAATGGTAGGAA+TGG	-	chr2.1:604961-604980	None:intergenic	40.0%
	CAAAAATGCCTCTATGACAG+AGG	-	chr2.1:605503-605522	None:intergenic	40.0%
	CTACTTACTGGAAAATGCTG+GGG	+	chr2.1:605555-605574	MS.gene90379:CDS	40.0%
	TAAATGCTACAGATGCTTCC+TGG	-	chr2.1:605177-605196	None:intergenic	40.0%
	TCAATAAGTCTCAAGAGACC+TGG	-	chr2.1:604718-604737	None:intergenic	40.0%
	TTGCCATGTTTGTGACATGA+AGG	+	chr2.1:605122-605141	MS.gene90379:intron	40.0%
!!!	CTCTGTCATAGAGGCATTTT+TGG	+	chr2.1:605501-605520	MS.gene90379:CDS	40.0%
!!!	GGTAATGTTTTGACCTGTTG+TGG	+	chr2.1:605143-605162	MS.gene90379:intron	40.0%
	ATGTGACCGTGATGAGCAAT+TGG	+	chr2.1:605525-605544	MS.gene90379:CDS	45.0%
	ATTGGCAGCCAACTACTTAC+TGG	+	chr2.1:605543-605562	MS.gene90379:CDS	45.0%
	CCTGTTGTGGTATCTAATCC+AGG	+	chr2.1:605156-605175	MS.gene90379:intron	45.0%
	TACCTATGACATGCAGCTAG+AGG	+	chr2.1:605459-605478	MS.gene90379:intron	45.0%
	TGATAGAGCCTCTGTCATAG+AGG	+	chr2.1:605492-605511	MS.gene90379:CDS	45.0%
!	CCTGGATTAGATACCACAAC+AGG	-	chr2.1:605159-605178	None:intergenic	45.0%
!	GGAAAATGCTGGGGATTTTG+AGG	+	chr2.1:605564-605583	MS.gene90379:CDS	45.0%
!!!	TTCTATTATAAATAATTTTT+CGG	+	chr2.1:604884-604903	MS.gene90379:intron	5.0%
	AGCCTCTAGCTGCATGTCAT+AGG	-	chr2.1:605464-605483	None:intergenic	50.0%
	ATGTGGGTCCTACCAACAAG+TGG	-	chr2.1:605026-605045	None:intergenic	50.0%
	GACATGCAGCTAGAGGCTAT+GGG	+	chr2.1:605466-605485	MS.gene90379:intron	50.0%
	GAGCTCGGAAAGCAAAATCC+AGG	+	chr2.1:604697-604716	MS.gene90379:CDS	50.0%
	GGCGTCACATTAACAGCATG+AGG	-	chr2.1:605297-605316	None:intergenic	50.0%
	TGACATGCAGCTAGAGGCTA+TGG	+	chr2.1:605465-605484	MS.gene90379:intron	50.0%
	TGATGTTGGGACCCACTTGT+TGG	+	chr2.1:605011-605030	MS.gene90379:intron	50.0%
	TGGCTGCCAATTGCTCATCA+CGG	-	chr2.1:605534-605553	None:intergenic	50.0%
	TGTGGGTCCTACCAACAAGT+GGG	-	chr2.1:605025-605044	None:intergenic	50.0%
	TTTCCGAGCTCCTGAAGTAC+AGG	-	chr2.1:604688-604707	None:intergenic	50.0%
!	AACCCATGGACGGTACTGAA+GGG	+	chr2.1:604773-604792	MS.gene90379:CDS	50.0%
	CACCCTTCAGTACCGTCCAT+GGG	-	chr2.1:604778-604797	None:intergenic	55.0%
	TAATGTGACGCCAGCTGAGC+AGG	+	chr2.1:605305-605324	MS.gene90379:CDS	55.0%
	TCACCCTTCAGTACCGTCCA+TGG	-	chr2.1:604779-604798	None:intergenic	55.0%
	TGAGCAGGAGGCAATCGGAA+GGG	+	chr2.1:605320-605339	MS.gene90379:CDS	55.0%
!	GAACCCATGGACGGTACTGA+AGG	+	chr2.1:604772-604791	MS.gene90379:CDS	55.0%
!	GTTGGGACCCACTTGTTGGT+AGG	+	chr2.1:605015-605034	MS.gene90379:intron	55.0%
	CCAGCTGAGCAGGAGGCAAT+CGG	+	chr2.1:605315-605334	MS.gene90379:CDS	60.0%
	CTGAGCAGGAGGCAATCGGA+AGG	+	chr2.1:605319-605338	MS.gene90379:CDS	60.0%
	CCGATTGCCTCCTGCTCAGC+TGG	-	chr2.1:605318-605337	None:intergenic	65.0%
	TGTGACGCCAGCTGAGCAGG+AGG	+	chr2.1:605308-605327	MS.gene90379:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	604685	605591	604685	ID=MS.gene90379
chr2.1	mRNA	604685	605591	604685	ID=MS.gene90379.t1;Parent=MS.gene90379
chr2.1	exon	604685	604794	604685	ID=MS.gene90379.t1.exon1;Parent=MS.gene90379.t1
chr2.1	CDS	604685	604794	604685	ID=cds.MS.gene90379.t1;Parent=MS.gene90379.t1
chr2.1	exon	605263	605341	605263	ID=MS.gene90379.t1.exon2;Parent=MS.gene90379.t1
chr2.1	CDS	605263	605341	605263	ID=cds.MS.gene90379.t1;Parent=MS.gene90379.t1
chr2.1	exon	605475	605591	605475	ID=MS.gene90379.t1.exon3;Parent=MS.gene90379.t1
chr2.1	CDS	605475	605591	605475	ID=cds.MS.gene90379.t1;Parent=MS.gene90379.t1

Gene Sequence

>MS.gene90379

CCTGTACTTCAGGAGCTCGGAAAGCAAAATCCAGGTCTCTTGAGACTTATTGACGAGCATCATTCTGAGTTTCTTCAGTTGATAAATGAACCCATGGACGGTACTGAAGGGTGAGTATACTGCTTTCCATGTATGGTCTGTGATAGTTTAACATGCCATCTTTGCATTTTACAATCATTACTTAATCCCATAGTCATGTTTCTATTATAAATAATTTTTCGGTTTTATTAGTTGTAGTCTTTGCCGAGTCAAGATCCTCTCCATTTTAGATCTCCATTCCTACCATTTGGAGAGATAAAGAAACAATACAATTTGAAACTAATTAATGATGTTGGGACCCACTTGTTGGTAGGACCCACATCTATATTTTGTGTGTATCTCTTTCTAAATGGCAATCTCCATTTATAAAAATAAATGGAGAGGATCCTAACTCGTCTTTGCCATGTTTGTGACATGAAGGTAATGTTTTGACCTGTTGTGGTATCTAATCCAGGAAGCATCTGTAGCATTTAAGATTGAAGTATTCATCTAGTAGTTTACTAGTTTATTTATAAATCTAACTCTCTTTCTTCTATTAGAGATAATTTTGATCAGGCGGAGCAAGACATGCCTCATGCTGTTAATGTGACGCCAGCTGAGCAGGAGGCAATCGGAAGGGTATATCTTTTTTTTTTTTTTTTTTAAGAATGATATGCGCTCTTTTCATTTCAATTGATTTACCTTATGCCTTTGCAAAAAAATGAAATTGATTTGCCATATGTGCTGATTCATTTTTACCTATGACATGCAGCTAGAGGCTATGGGATTTGATAGAGCCTCTGTCATAGAGGCATTTTTGGCATGTGACCGTGATGAGCAATTGGCAGCCAACTACTTACTGGAAAATGCTGGGGATTTTGAGGATTAA

Protein sequence

>MS.gene90379.t1

PVLQELGKQNPGLLRLIDEHHSEFLQLINEPMDGTEGDNFDQAEQDMPHAVNVTPAEQEAIGRLEAMGFDRASVIEAFLACDRDEQLAANYLLENAGDFED