AlfalfaGEDB | MS.gene059900

M. sativa cultivar XinJiangDaYe / MS.gene059900

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059900.t1	XP_003596481.1	94.9	117	6	0	1	117	1	117	2.70E-52	214.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059900.t1	G7IR51	94.9	117	6	0	1	117	1	117	2.0e-52	214.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057102	MS.gene059900	-0.816355	5.77E-52	-1.69E-46
MS.gene05985	MS.gene059900	0.805507	1.29E-49	-1.69E-46
MS.gene059900	MS.gene060338	-0.810888	9.22E-51	-1.69E-46
MS.gene059900	MS.gene061188	-0.801707	7.93E-49	-1.69E-46
MS.gene059900	MS.gene067770	-0.840559	8.09E-58	-1.69E-46
MS.gene059900	MS.gene07325	-0.807589	4.69E-50	-1.69E-46
MS.gene059900	MS.gene073846	-0.809704	1.66E-50	-1.69E-46
MS.gene059900	MS.gene07529	-0.81185	5.70E-51	-1.69E-46
MS.gene059900	MS.gene09934	-0.818637	1.77E-52	-1.69E-46
MS.gene059900	MS.gene20874	-0.809542	1.80E-50	-1.69E-46
MS.gene059900	MS.gene25806	-0.82379	1.15E-53	-1.69E-46
MS.gene059900	MS.gene27646	0.820311	7.34E-53	-1.69E-46
MS.gene059900	MS.gene29374	0.818749	1.67E-52	-1.69E-46
MS.gene059900	MS.gene29631	-0.808597	2.86E-50	-1.69E-46
MS.gene059900	MS.gene32893	-0.824059	9.91E-54	-1.69E-46
MS.gene059900	MS.gene33650	-0.811009	8.67E-51	-1.69E-46
MS.gene059900	MS.gene34950	-0.803209	3.89E-49	-1.69E-46
MS.gene059900	MS.gene41689	-0.842837	2.03E-58	-1.69E-46
MS.gene059900	MS.gene47759	-0.805842	1.10E-49	-1.69E-46
MS.gene059900	MS.gene47822	-0.811297	7.51E-51	-1.69E-46
MS.gene059900	MS.gene48037	-0.818522	1.88E-52	-1.69E-46
MS.gene059900	MS.gene51910	-0.829106	6.20E-55	-1.69E-46
MS.gene059900	MS.gene51947	-0.841443	4.74E-58	-1.69E-46
MS.gene059900	MS.gene55785	0.813173	2.93E-51	-1.69E-46
MS.gene059900	MS.gene55818	-0.804742	1.87E-49	-1.69E-46
MS.gene059900	MS.gene58081	-0.805817	1.11E-49	-1.69E-46
MS.gene059900	MS.gene69780	-0.822774	1.98E-53	-1.69E-46
MS.gene059900	MS.gene71891	0.858028	1.09E-62	-1.69E-46
MS.gene059900	MS.gene83864	-0.811388	7.18E-51	-1.69E-46
MS.gene059900	MS.gene88373	0.991246	1.22E-186	-1.69E-46
MS.gene059900	MS.gene90379	-0.84381	1.11E-58	-1.69E-46
MS.gene059900	MS.gene90874	-0.804927	1.71E-49	-1.69E-46
MS.gene059900	MS.gene91485	-0.81087	9.30E-51	-1.69E-46
MS.gene059900	MS.gene95145	-0.80332	3.69E-49	-1.69E-46
MS.gene059900	MS.gene96286	-0.819168	1.34E-52	-1.69E-46
MS.gene059900	MS.gene99949	-0.819449	1.15E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059900.t1	MTR_2g081000	94.872	117	6	0	1	117	1	117	1.82e-65	207

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 41 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGCGCCTCTTTCCGCTTC+CGG	0.265737	2.2:+15067138	MS.gene059900:CDS
GGGAGTTTAGGGAAGGGTTT+TGG	0.293170	2.2:-15066997	None:intergenic
TACGGAGGAGAGGGAGTTTA+GGG	0.300913	2.2:-15067008	None:intergenic
TTACGGAGGAGAGGGAGTTT+AGG	0.308680	2.2:-15067009	None:intergenic
GAAGGGATGGATGAGTTCAT+TGG	0.350804	2.2:-15066961	None:intergenic
CCGTATTTACCTTTCATAAC+CGG	0.395087	2.2:-15067171	None:intergenic
CCGTTCAAGCAGCGAGGAAC+CGG	0.396666	2.2:-15067100	None:intergenic
CATAACCGGATTAATGTTAC+CGG	0.413040	2.2:-15067157	None:intergenic
GAAGCGGAAAGAGGCGCGGC+CGG	0.424508	2.2:-15067135	None:intergenic
AAAGGTAAATACGGTGCGTT+TGG	0.441403	2.2:+15067180	MS.gene059900:CDS
CGCTTCCGGTAACATTAATC+CGG	0.441871	2.2:+15067152	MS.gene059900:CDS
CCGGTTCCTCGCTGCTTGAA+CGG	0.442423	2.2:+15067100	MS.gene059900:CDS
AAGGGATGGATGAGTTCATT+GGG	0.454914	2.2:-15066960	None:intergenic
CGGATTAATGTTACCGGAAG+CGG	0.470374	2.2:-15067151	None:intergenic
CAAGACGGCGGTGACGGTTA+CGG	0.473141	2.2:-15067026	None:intergenic
TGAACGGTGTTTCGCTGCTC+CGG	0.487700	2.2:+15067116	MS.gene059900:CDS
CCGGTTATGAAAGGTAAATA+CGG	0.497596	2.2:+15067171	MS.gene059900:CDS
CGGGAGAAGCGGACCACGAT+CGG	0.509858	2.2:-15067053	None:intergenic
GAGGAGAGGGAGTTTAGGGA+AGG	0.512666	2.2:-15067004	None:intergenic
ATATCGCTGTCGACATCGGC+GGG	0.513440	2.2:-15067072	None:intergenic
ATGTTACCGGAAGCGGAAAG+AGG	0.528493	2.2:-15067144	None:intergenic
AACATTAATCCGGTTATGAA+AGG	0.530557	2.2:+15067162	MS.gene059900:CDS
AGGAGAGGGAGTTTAGGGAA+GGG	0.533110	2.2:-15067003	None:intergenic
GTCGACAGCGATATTTCCTC+CGG	0.540129	2.2:+15067081	MS.gene059900:CDS
GTCGACATCGGCGGGAGAAG+CGG	0.545373	2.2:-15067064	None:intergenic
CTTCGATTTCTCGAGCGTAA+CGG	0.550925	2.2:-15067205	None:intergenic
GGAAATATCGCTGTCGACAT+CGG	0.555376	2.2:-15067076	None:intergenic
GGATAACAAGACGGCGGTGA+CGG	0.559797	2.2:-15067032	None:intergenic
AAAGAAATATGACAAACCTC+AGG	0.569697	2.2:-15067264	None:intergenic
GGTGACGGTTACGGAGGAGA+GGG	0.577402	2.2:-15067017	None:intergenic
GGATGGATGAGTTCATTGGG+AGG	0.588310	2.2:-15066957	None:intergenic
AAGTAACAAGTCTGCGCCTG+AGG	0.601985	2.2:+15067248	MS.gene059900:CDS
CGGTGACGGTTACGGAGGAG+AGG	0.603340	2.2:-15067018	None:intergenic
TTCAAGCAGCGAGGAACCGG+AGG	0.621943	2.2:-15067097	None:intergenic
AATATCGCTGTCGACATCGG+CGG	0.624159	2.2:-15067073	None:intergenic
ACCGGAAGCGGAAAGAGGCG+CGG	0.630373	2.2:-15067139	None:intergenic
GACGGCGGTGACGGTTACGG+AGG	0.646221	2.2:-15067023	None:intergenic
GCCGTCTTGTTATCCGATCG+TGG	0.647592	2.2:+15067040	MS.gene059900:CDS
ACCACGATCGGATAACAAGA+CGG	0.656119	2.2:-15067041	None:intergenic
GAAACACCGTTCAAGCAGCG+AGG	0.728116	2.2:-15067106	None:intergenic
ACGATCGGATAACAAGACGG+CGG	0.806476	2.2:-15067038	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAGAAATATGACAAACCTC+AGG	-	chr2.2:15067267-15067286	None:intergenic	30.0%
	AACATTAATCCGGTTATGAA+AGG	+	chr2.2:15067162-15067181	MS.gene059900:CDS	30.0%
!!!	TTGGTGATAAGGTTTTAGAA+GGG	-	chr2.2:15066981-15067000	None:intergenic	30.0%
!!!	TTTGGTGATAAGGTTTTAGA+AGG	-	chr2.2:15066982-15067001	None:intergenic	30.0%
	CATAACCGGATTAATGTTAC+CGG	-	chr2.2:15067160-15067179	None:intergenic	35.0%
	CCGGTTATGAAAGGTAAATA+CGG	+	chr2.2:15067171-15067190	MS.gene059900:CDS	35.0%
	CCGTATTTACCTTTCATAAC+CGG	-	chr2.2:15067174-15067193	None:intergenic	35.0%
!!!	TGATAAGGTTTTAGAAGGGA+TGG	-	chr2.2:15066977-15066996	None:intergenic	35.0%
	AAAGGTAAATACGGTGCGTT+TGG	+	chr2.2:15067180-15067199	MS.gene059900:CDS	40.0%
	AAGGGATGGATGAGTTCATT+GGG	-	chr2.2:15066963-15066982	None:intergenic	40.0%
	ACCACGATCGGATAACAAGA+CGG	-	chr2.2:15067044-15067063	None:intergenic	45.0%
	CGCTTCCGGTAACATTAATC+CGG	+	chr2.2:15067152-15067171	MS.gene059900:CDS	45.0%
	CGGATTAATGTTACCGGAAG+CGG	-	chr2.2:15067154-15067173	None:intergenic	45.0%
	CTTCGATTTCTCGAGCGTAA+CGG	-	chr2.2:15067208-15067227	None:intergenic	45.0%
	GAAGGGATGGATGAGTTCAT+TGG	-	chr2.2:15066964-15066983	None:intergenic	45.0%
	GGAAATATCGCTGTCGACAT+CGG	-	chr2.2:15067079-15067098	None:intergenic	45.0%
!!	AGGGAAGGGTTTTGGTGATA+AGG	-	chr2.2:15066992-15067011	None:intergenic	45.0%
	AAGTAACAAGTCTGCGCCTG+AGG	+	chr2.2:15067248-15067267	MS.gene059900:CDS	50.0%
	AATATCGCTGTCGACATCGG+CGG	-	chr2.2:15067076-15067095	None:intergenic	50.0%
	ACGATCGGATAACAAGACGG+CGG	-	chr2.2:15067041-15067060	None:intergenic	50.0%
	AGGAGAGGGAGTTTAGGGAA+GGG	-	chr2.2:15067006-15067025	None:intergenic	50.0%
	ATGTTACCGGAAGCGGAAAG+AGG	-	chr2.2:15067147-15067166	None:intergenic	50.0%
	GGATGGATGAGTTCATTGGG+AGG	-	chr2.2:15066960-15066979	None:intergenic	50.0%
	GTCGACAGCGATATTTCCTC+CGG	+	chr2.2:15067081-15067100	MS.gene059900:CDS	50.0%
	TACGGAGGAGAGGGAGTTTA+GGG	-	chr2.2:15067011-15067030	None:intergenic	50.0%
	TTACGGAGGAGAGGGAGTTT+AGG	-	chr2.2:15067012-15067031	None:intergenic	50.0%
!	GGGAGTTTAGGGAAGGGTTT+TGG	-	chr2.2:15067000-15067019	None:intergenic	50.0%
	ATATCGCTGTCGACATCGGC+GGG	-	chr2.2:15067075-15067094	None:intergenic	55.0%
	GAAACACCGTTCAAGCAGCG+AGG	-	chr2.2:15067109-15067128	None:intergenic	55.0%
	GAGGAGAGGGAGTTTAGGGA+AGG	-	chr2.2:15067007-15067026	None:intergenic	55.0%
	GGATAACAAGACGGCGGTGA+CGG	-	chr2.2:15067035-15067054	None:intergenic	55.0%
	TGAACGGTGTTTCGCTGCTC+CGG	+	chr2.2:15067116-15067135	MS.gene059900:CDS	55.0%
!	GCCGTCTTGTTATCCGATCG+TGG	+	chr2.2:15067040-15067059	MS.gene059900:CDS	55.0%
	CCGGTTCCTCGCTGCTTGAA+CGG	+	chr2.2:15067100-15067119	MS.gene059900:CDS	60.0%
	CCGTTCAAGCAGCGAGGAAC+CGG	-	chr2.2:15067103-15067122	None:intergenic	60.0%
	TTCAAGCAGCGAGGAACCGG+AGG	-	chr2.2:15067100-15067119	None:intergenic	60.0%
!	CAAGACGGCGGTGACGGTTA+CGG	-	chr2.2:15067029-15067048	None:intergenic	60.0%
!	GGTGACGGTTACGGAGGAGA+GGG	-	chr2.2:15067020-15067039	None:intergenic	60.0%
	ACCGGAAGCGGAAAGAGGCG+CGG	-	chr2.2:15067142-15067161	None:intergenic	65.0%
	CGGGAGAAGCGGACCACGAT+CGG	-	chr2.2:15067056-15067075	None:intergenic	65.0%
	GTCGACATCGGCGGGAGAAG+CGG	-	chr2.2:15067067-15067086	None:intergenic	65.0%
!	CGGTGACGGTTACGGAGGAG+AGG	-	chr2.2:15067021-15067040	None:intergenic	65.0%
	GCCGCGCCTCTTTCCGCTTC+CGG	+	chr2.2:15067138-15067157	MS.gene059900:CDS	70.0%
!	GAAGCGGAAAGAGGCGCGGC+CGG	-	chr2.2:15067138-15067157	None:intergenic	70.0%
!	GACGGCGGTGACGGTTACGG+AGG	-	chr2.2:15067026-15067045	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	15066922	15067299	15066922	ID=MS.gene059900
chr2.2	mRNA	15066922	15067299	15066922	ID=MS.gene059900.t1;Parent=MS.gene059900
chr2.2	exon	15066922	15067299	15066922	ID=MS.gene059900.t1.exon1;Parent=MS.gene059900.t1
chr2.2	CDS	15066922	15067299	15066922	ID=cds.MS.gene059900.t1;Parent=MS.gene059900.t1

Gene Sequence	>MS.gene059900 ATGTCGTCTCACGCGCTGTTTCAAGCAGCGCAAATCCTCCCAATGAACTCATCCATCCCTTCTAAAACCTTATCACCAAAACCCTTCCCTAAACTCCCTCTCCTCCGTAACCGTCACCGCCGTCTTGTTATCCGATCGTGGTCCGCTTCTCCCGCCGATGTCGACAGCGATATTTCCTCCGGTTCCTCGCTGCTTGAACGGTGTTTCGCTGCTCCGGCCGCGCCTCTTTCCGCTTCCGGTAACATTAATCCGGTTATGAAAGGTAAATACGGTGCGTTTGGTGCCGTTACGCTCGAGAAATCGAAGCTTGAAATTTCGCAACAACAAAGTAACAAGTCTGCGCCTGAGGTTTGTCATATTTCTTTCTTTCTTCACTAG
Protein sequence	>MS.gene059900.t1 MSSHALFQAAQILPMNSSIPSKTLSPKPFPKLPLLRNRHRRLVIRSWSASPADVDSDISSGSSLLERCFAAPAAPLSASGNINPVMKGKYGAFGAVTLEKSKLEISQQQSNKSAPEVCHISFFLH