AlfalfaGEDB | MS.gene053540

M. sativa cultivar XinJiangDaYe / MS.gene053540

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053540.t1	XP_003607925.1	94.1	101	6	0	1	101	898	998	5.10E-41	176.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053540.t1	G7JK12	94.1	101	6	0	1	101	898	998	3.7e-41	176.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053536	MS.gene053540	0.919101	6.85E-87	-1.69E-46
MS.gene053538	MS.gene053540	0.926204	6.46E-91	-1.69E-46
MS.gene053540	MS.gene05894	0.833912	4.06E-56	-1.69E-46
MS.gene053540	MS.gene059424	0.90297	5.62E-79	-1.69E-46
MS.gene053540	MS.gene059425	0.856294	3.55E-62	-1.69E-46
MS.gene053540	MS.gene06890	0.815963	7.06E-52	-1.69E-46
MS.gene053540	MS.gene07723	0.804581	2.02E-49	-1.69E-46
MS.gene053540	MS.gene26380	0.808848	2.53E-50	-1.69E-46
MS.gene053540	MS.gene26464	0.894289	2.84E-75	-1.69E-46
MS.gene053540	MS.gene26465	0.85476	9.93E-62	-1.69E-46
MS.gene053540	MS.gene26659	0.806103	9.68E-50	-1.69E-46
MS.gene053540	MS.gene32854	0.80635	8.59E-50	-1.69E-46
MS.gene053540	MS.gene36880	0.815911	7.25E-52	-1.69E-46
MS.gene053540	MS.gene37460	0.811063	8.44E-51	-1.69E-46
MS.gene053540	MS.gene39297	0.837241	5.84E-57	-1.69E-46
MS.gene053540	MS.gene41651	0.807524	4.85E-50	-1.69E-46
MS.gene053540	MS.gene41689	0.820666	6.08E-53	-1.69E-46
MS.gene053540	MS.gene44437	0.831733	1.41E-55	-1.69E-46
MS.gene053540	MS.gene47586	0.804148	2.48E-49	-1.69E-46
MS.gene053540	MS.gene55101	0.830044	3.67E-55	-1.69E-46
MS.gene053540	MS.gene57130	0.814005	1.92E-51	-1.69E-46
MS.gene053540	MS.gene60174	0.830337	3.11E-55	-1.69E-46
MS.gene053540	MS.gene78758	0.807981	3.88E-50	-1.69E-46
MS.gene053540	MS.gene90379	0.823743	1.17E-53	-1.69E-46
MS.gene053540	MS.gene99949	0.828848	7.16E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053540.t1	MTR_4g085570	94.059	101	6	0	1	101	898	998	1.77e-60	198
MS.gene053540.t1	MTR_4g017520	71.845	103	26	1	2	101	113	215	3.56e-45	145
MS.gene053540.t1	MTR_2g104465	77.941	68	14	1	14	80	1	68	6.89e-30	102

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCCTTCTTCAATCTGAAAA+TGG	0.240730	4.3:-27239931	MS.gene053540:CDS
TACAATTGCTAGATGTCCTT+TGG	0.364051	4.3:+27239858	None:intergenic
CATCATCCTCGGCTTGCATA+TGG	0.403033	4.3:+27240022	None:intergenic
TGACTTCAAAGCAAAATGAT+AGG	0.439035	4.3:-27239888	MS.gene053540:CDS
CTCTTCTTTAACATCATCCT+CGG	0.449126	4.3:+27240011	None:intergenic
AGAAAGAAAGGGAGGAGTAG+AGG	0.460861	4.3:-27239784	MS.gene053540:CDS
CAGCTTCCTCAGAAAATCAA+AGG	0.547753	4.3:+27239816	None:intergenic
TTGCATATGGTTGAGTAGTG+AGG	0.619906	4.3:+27240035	None:intergenic
AATGATAGGAGCAATTCCAA+AGG	0.669148	4.3:-27239874	MS.gene053540:CDS
ATTGTACCGAGGAGAAAACA+AGG	0.675964	4.3:-27239841	MS.gene053540:CDS
ACTCAACCATATGCAAGCCG+AGG	0.681575	4.3:-27240028	MS.gene053540:CDS
GACATCTAGCAATTGTACCG+AGG	0.698621	4.3:-27239852	MS.gene053540:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTTTTTAAGAAGAAAGAAA+GGG	-	chr4.3:27240036-27240055	MS.gene053540:CDS	20.0%
!!!	CTGTTTTTAAGAAGAAAGAA+AGG	-	chr4.3:27240035-27240054	MS.gene053540:CDS	25.0%
	TGACTTCAAAGCAAAATGAT+AGG	-	chr4.3:27239943-27239962	MS.gene053540:CDS	30.0%
!	TCCATTTTCAGATTGAAGAA+GGG	+	chr4.3:27239904-27239923	None:intergenic	30.0%
!	TTCCATTTTCAGATTGAAGA+AGG	+	chr4.3:27239905-27239924	None:intergenic	30.0%
!!	TTTTAAGAAGAAAGAAAGGG+AGG	-	chr4.3:27240039-27240058	MS.gene053540:CDS	30.0%
	AATGATAGGAGCAATTCCAA+AGG	-	chr4.3:27239957-27239976	MS.gene053540:CDS	35.0%
	CTCTTCTTTAACATCATCCT+CGG	+	chr4.3:27239823-27239842	None:intergenic	35.0%
	TACAATTGCTAGATGTCCTT+TGG	+	chr4.3:27239976-27239995	None:intergenic	35.0%
!	CCATTTTCAGATTGAAGAAG+GGG	+	chr4.3:27239903-27239922	None:intergenic	35.0%
	ATTGTACCGAGGAGAAAACA+AGG	-	chr4.3:27239990-27240009	MS.gene053540:CDS	40.0%
	CAGCTTCCTCAGAAAATCAA+AGG	+	chr4.3:27240018-27240037	None:intergenic	40.0%
	CCCCTTCTTCAATCTGAAAA+TGG	-	chr4.3:27239900-27239919	MS.gene053540:CDS	40.0%
	TTGCATATGGTTGAGTAGTG+AGG	+	chr4.3:27239799-27239818	None:intergenic	40.0%
!	TTTTCAGATTGAAGAAGGGG+AGG	+	chr4.3:27239900-27239919	None:intergenic	40.0%
!!	AAGGAGCCTTTGATTTTCTG+AGG	-	chr4.3:27240009-27240028	MS.gene053540:CDS	40.0%
	AGAAAGAAAGGGAGGAGTAG+AGG	-	chr4.3:27240047-27240066	MS.gene053540:CDS	45.0%
	GACATCTAGCAATTGTACCG+AGG	-	chr4.3:27239979-27239998	MS.gene053540:CDS	45.0%
!!!	AAGGCTCCTTGTTTTCTCCT+CGG	+	chr4.3:27239999-27240018	None:intergenic	45.0%
	ACTCAACCATATGCAAGCCG+AGG	-	chr4.3:27239803-27239822	MS.gene053540:CDS	50.0%
!	CATCATCCTCGGCTTGCATA+TGG	+	chr4.3:27239812-27239831	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	27239774	27240079	27239774	ID=MS.gene053540
chr4.3	mRNA	27239774	27240079	27239774	ID=MS.gene053540.t1;Parent=MS.gene053540
chr4.3	exon	27239774	27240079	27239774	ID=MS.gene053540.t1.exon1;Parent=MS.gene053540.t1
chr4.3	CDS	27239774	27240079	27239774	ID=cds.MS.gene053540.t1;Parent=MS.gene053540.t1

Gene Sequence	>MS.gene053540 AAGAAGAACTTGATCAAACGATCCTCACTACTCAACCATATGCAAGCCGAGGATGATGTTAAAGAAGAGATGCATAAGTCTGTTGAAAAAGAAGAAAGCAAAACTGCTGAAGATGCACAACAGCCTCCCCTTCTTCAATCTGAAAATGGAACTGTTTTGCAAACAGATATGACTTCAAAGCAAAATGATAGGAGCAATTCCAAAGGACATCTAGCAATTGTACCGAGGAGAAAACAAGGAGCCTTTGATTTTCTGAGGAAGCTGTTTTTAAGAAGAAAGAAAGGGAGGAGTAGAGGAACAAGATAG
Protein sequence	>MS.gene053540.t1 KKNLIKRSSLLNHMQAEDDVKEEMHKSVEKEESKTAEDAQQPPLLQSENGTVLQTDMTSKQNDRSNSKGHLAIVPRRKQGAFDFLRKLFLRRKKGRSRGTR