Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051018.t1 | XP_003627466.2 | 94.5 | 164 | 9 | 0 | 1 | 164 | 1 | 164 | 9.70E-82 | 312.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051018.t1 | G7L9W5 | 94.5 | 164 | 9 | 0 | 1 | 164 | 1 | 164 | 7.0e-82 | 312.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene051018 | 0.802186 | 6.32E-49 | -1.69E-46 |
| MS.gene049418 | MS.gene051018 | 0.812183 | 4.82E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene051018 | 0.822978 | 1.77E-53 | -1.69E-46 |
| MS.gene049850 | MS.gene051018 | 0.804174 | 2.45E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene051664 | 0.836606 | 8.48E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene051719 | 0.823646 | 1.24E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene051720 | 0.838692 | 2.47E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene053144 | 0.848938 | 4.45E-60 | -1.69E-46 |
| MS.gene051018 | MS.gene053536 | 0.833432 | 5.35E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene053538 | 0.832483 | 9.22E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene054584 | 0.834185 | 3.47E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene055083 | 0.80468 | 1.92E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene055465 | 0.826527 | 2.58E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene055466 | 0.834792 | 2.44E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene055468 | 0.811355 | 7.30E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene055537 | 0.849167 | 3.85E-60 | -1.69E-46 |
| MS.gene051018 | MS.gene055580 | -0.812217 | 4.74E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene055764 | 0.814723 | 1.33E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene056169 | 0.812399 | 4.32E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene057082 | 0.805147 | 1.54E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene057294 | 0.801782 | 7.65E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene05894 | 0.831292 | 1.81E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene059424 | 0.82539 | 4.81E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene060519 | 0.819705 | 1.01E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene061335 | 0.808102 | 3.65E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene061840 | 0.831601 | 1.52E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene061866 | 0.803544 | 3.31E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene061869 | 0.815927 | 7.19E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene061872 | 0.827987 | 1.15E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene061874 | 0.807491 | 4.92E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene062043 | 0.80258 | 5.24E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene06258 | 0.856441 | 3.21E-62 | -1.69E-46 |
| MS.gene051018 | MS.gene062732 | 0.805201 | 1.50E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene062743 | 0.813108 | 3.02E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene064520 | 0.834843 | 2.37E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene064521 | 0.83741 | 5.29E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene064522 | 0.820682 | 6.03E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene06469 | 0.805679 | 1.19E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene065667 | 0.818468 | 1.93E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene065674 | 0.801174 | 1.02E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene065979 | 0.828157 | 1.05E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene066010 | 0.810592 | 1.07E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene066105 | 0.811335 | 7.37E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene066782 | 0.818408 | 1.99E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene066784 | 0.814407 | 1.57E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene06793 | 0.821998 | 3.00E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene069852 | 0.847585 | 1.05E-59 | -1.69E-46 |
| MS.gene051018 | MS.gene069884 | 0.820536 | 6.51E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene069966 | 0.806781 | 6.96E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene071490 | 0.832079 | 1.16E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene071652 | 0.80091 | 1.15E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene07246 | 0.836127 | 1.12E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene073262 | 0.826771 | 2.26E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene073326 | 0.813384 | 2.63E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene07359 | 0.83369 | 4.61E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene07374 | 0.811332 | 7.38E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene074519 | 0.829046 | 6.41E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene07723 | 0.839837 | 1.25E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene07876 | 0.808381 | 3.19E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene09575 | 0.802421 | 5.66E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene09621 | 0.81768 | 2.91E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene09873 | 0.806918 | 6.51E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene20903 | 0.828403 | 9.17E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene20920 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene21088 | 0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene21127 | 0.824295 | 8.72E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene21144 | 0.803087 | 4.12E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene21960 | 0.831588 | 1.53E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene22470 | 0.800795 | 1.22E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene22941 | 0.804049 | 2.60E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene23837 | 0.802872 | 4.57E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene23880 | 0.818533 | 1.87E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene24507 | 0.809765 | 1.61E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene24736 | 0.814943 | 1.19E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene24822 | 0.83097 | 2.18E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene26380 | 0.850993 | 1.19E-60 | -1.69E-46 |
| MS.gene051018 | MS.gene26464 | 0.826944 | 2.05E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene26465 | 0.821438 | 4.04E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene26659 | 0.851788 | 7.06E-61 | -1.69E-46 |
| MS.gene051018 | MS.gene27646 | -0.816201 | 6.25E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene27860 | 0.807209 | 5.65E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene29807 | 0.804758 | 1.85E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene30739 | 0.80137 | 9.30E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene30882 | 0.80291 | 4.48E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene30946 | 0.800602 | 1.33E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene31433 | 0.834342 | 3.17E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene32024 | 0.841196 | 5.50E-58 | -1.69E-46 |
| MS.gene051018 | MS.gene33123 | 0.828158 | 1.05E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene34286 | 0.843156 | 1.67E-58 | -1.69E-46 |
| MS.gene051018 | MS.gene34979 | 0.805131 | 1.55E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene35575 | 0.80671 | 7.21E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene36265 | 0.823073 | 1.69E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene36334 | 0.820257 | 7.55E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene38303 | 0.842135 | 3.11E-58 | -1.69E-46 |
| MS.gene051018 | MS.gene38561 | 0.812705 | 3.71E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene38829 | 0.83613 | 1.12E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene39518 | 0.831488 | 1.62E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene40409 | 0.826245 | 3.02E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene41651 | 0.87236 | 3.38E-67 | -1.69E-46 |
| MS.gene051018 | MS.gene41859 | 0.80163 | 8.22E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene43178 | 0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene44437 | 0.836051 | 1.17E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene44783 | 0.80104 | 1.09E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene45217 | 0.800772 | 1.23E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene45425 | 0.81586 | 7.44E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene46189 | 0.809214 | 2.11E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene47694 | 0.826092 | 3.28E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene48346 | 0.822434 | 2.37E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene49313 | 0.813045 | 3.12E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene49378 | 0.807955 | 3.92E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene50564 | 0.809422 | 1.91E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene51589 | 0.831842 | 1.33E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene52952 | 0.804302 | 2.31E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene52996 | 0.801853 | 7.40E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene53376 | 0.806025 | 1.00E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene54967 | 0.800338 | 1.51E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene55101 | 0.845235 | 4.61E-59 | -1.69E-46 |
| MS.gene051018 | MS.gene55425 | 0.800364 | 1.49E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene56169 | 0.833456 | 5.28E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene57130 | 0.808497 | 3.01E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene57665 | 0.807447 | 5.03E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene58836 | 0.839324 | 1.70E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene60174 | 0.841394 | 4.88E-58 | -1.69E-46 |
| MS.gene051018 | MS.gene60685 | 0.802848 | 4.62E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene61226 | 0.817072 | 3.99E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene61720 | 0.80559 | 1.24E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene62491 | 0.832523 | 9.00E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene62806 | 0.814393 | 1.58E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene62916 | 0.813172 | 2.93E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene64763 | 0.835274 | 1.85E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene65271 | 0.811756 | 5.97E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene66156 | 0.803468 | 3.44E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene66409 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene68442 | 0.802613 | 5.16E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene68852 | 0.830487 | 2.86E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene68949 | 0.83857 | 2.66E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene69459 | 0.822631 | 2.14E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene69993 | 0.807184 | 5.72E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene71440 | 0.80236 | 5.82E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene71704 | 0.813736 | 2.20E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene71807 | 0.806391 | 8.41E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene72135 | 0.80512 | 1.56E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene72487 | 0.818954 | 1.50E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene75205 | 0.812775 | 3.58E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene75387 | 0.805312 | 1.42E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene75670 | 0.81269 | 3.73E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene75964 | 0.833883 | 4.13E-56 | -1.69E-46 |
| MS.gene051018 | MS.gene76516 | 0.804146 | 2.49E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene76518 | 0.858914 | 5.94E-63 | -1.69E-46 |
| MS.gene051018 | MS.gene76549 | 0.817409 | 3.35E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene77734 | 0.811042 | 8.53E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene78483 | 0.801655 | 8.13E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene78530 | 0.810055 | 1.39E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene78578 | 0.801481 | 8.82E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene78652 | 0.824541 | 7.63E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene78729 | 0.819808 | 9.56E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene80266 | 0.831423 | 1.68E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene80844 | 0.806706 | 7.22E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene81329 | 0.803199 | 3.91E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene83055 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene051018 | MS.gene84426 | 0.837399 | 5.32E-57 | -1.69E-46 |
| MS.gene051018 | MS.gene85066 | 0.812203 | 4.77E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene85133 | 0.856461 | 3.17E-62 | -1.69E-46 |
| MS.gene051018 | MS.gene85164 | 0.840459 | 8.59E-58 | -1.69E-46 |
| MS.gene051018 | MS.gene85553 | 0.826072 | 3.32E-54 | -1.69E-46 |
| MS.gene051018 | MS.gene86086 | 0.810359 | 1.20E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene88171 | 0.804639 | 1.96E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene88304 | 0.802955 | 4.39E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene90379 | 0.803747 | 3.01E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene90674 | 0.818366 | 2.04E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene90906 | 0.814634 | 1.39E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene92982 | 0.80126 | 9.79E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene94764 | 0.803189 | 3.92E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene95438 | 0.820865 | 5.47E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene96315 | 0.813662 | 2.29E-51 | -1.69E-46 |
| MS.gene051018 | MS.gene97017 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene97306 | 0.821054 | 4.95E-53 | -1.69E-46 |
| MS.gene051018 | MS.gene98735 | 0.808163 | 3.54E-50 | -1.69E-46 |
| MS.gene051018 | MS.gene99949 | 0.834223 | 3.39E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051018.t1 | MTR_8g023390 | 94.512 | 164 | 9 | 0 | 1 | 164 | 1 | 164 | 7.67e-114 | 320 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAACAAACTTGTTATTAA+AGG | 0.220128 | 8.3:+68788476 | None:intergenic |
| GTTATTAAAGGTCCAGAAAA+TGG | 0.227908 | 8.3:+68788488 | None:intergenic |
| TTTAGCTATAGATTTGTTAT+TGG | 0.228772 | 8.3:+68788589 | None:intergenic |
| TTCTCTTCTGATCTCTTTGT+TGG | 0.245231 | 8.3:+68788452 | None:intergenic |
| CTTCCCATCATAACCTTTGT+AGG | 0.252175 | 8.3:+68788520 | None:intergenic |
| AAAAGACAAGGCCATGTTTC+TGG | 0.267409 | 8.3:-68788557 | MS.gene051018:CDS |
| TTTCCCTTTCTCACTTTCTC+TGG | 0.320462 | 8.3:+68788680 | None:intergenic |
| TGTTGATGGAGAGTCATATA+AGG | 0.356203 | 8.3:+68788422 | None:intergenic |
| CCAAGGCATTATGATGACTA+TGG | 0.364903 | 8.3:-68789314 | MS.gene051018:CDS |
| GGAAAAGTTCCCTGGTTTCA+TGG | 0.375096 | 8.3:-68788649 | MS.gene051018:CDS |
| CCTCATCCTGTTCAAAACTA+TGG | 0.408153 | 8.3:-68788392 | MS.gene051018:CDS |
| AACTCCTACAAAGGTTATGA+TGG | 0.409233 | 8.3:-68788524 | MS.gene051018:CDS |
| CCTTTGTAGGAGTTAAGTAT+TGG | 0.416425 | 8.3:+68788533 | None:intergenic |
| AGGACCAGAGAAAGTGAGAA+AGG | 0.425794 | 8.3:-68788684 | MS.gene051018:CDS |
| CTATGGATTTGATCTGCAAC+AGG | 0.457681 | 8.3:-68789297 | MS.gene051018:CDS |
| AAACTATGGTCCTCTTTATG+TGG | 0.476561 | 8.3:-68788378 | MS.gene051018:CDS |
| TGAGAAAGGGAAAGAAATCA+TGG | 0.486173 | 8.3:-68788670 | MS.gene051018:CDS |
| GGACCAGAGAAAGTGAGAAA+GGG | 0.486612 | 8.3:-68788683 | MS.gene051018:CDS |
| CAAGTTTCCACCATGAAACC+AGG | 0.489863 | 8.3:+68788639 | None:intergenic |
| CTAGTTTCTGGCTGAAGCAA+AGG | 0.513346 | 8.3:-68788771 | MS.gene051018:intron |
| AGTTAAGTAACCACATAAAG+AGG | 0.516609 | 8.3:+68788368 | None:intergenic |
| GAAACTTGACAAGAAAAGTA+AGG | 0.522042 | 8.3:-68788624 | MS.gene051018:CDS |
| GAAATCATGGAAAAGTTCCC+TGG | 0.522925 | 8.3:-68788657 | MS.gene051018:CDS |
| CCATAGTCATCATAATGCCT+TGG | 0.544319 | 8.3:+68789314 | None:intergenic |
| CCAATACTTAACTCCTACAA+AGG | 0.560845 | 8.3:-68788533 | MS.gene051018:CDS |
| TCAACAGTGTACCCTGAAGA+AGG | 0.568735 | 8.3:-68788719 | MS.gene051018:CDS |
| ACTCCTACAAAGGTTATGAT+GGG | 0.578643 | 8.3:-68788523 | MS.gene051018:CDS |
| CTGGCTGAAGCAAAGGAGCA+TGG | 0.602825 | 8.3:-68788764 | MS.gene051018:CDS |
| TAAGTATTGGACCAGAAACA+TGG | 0.636616 | 8.3:+68788546 | None:intergenic |
| AAAGTTCCCTGGTTTCATGG+TGG | 0.673568 | 8.3:-68788646 | MS.gene051018:CDS |
| ACAATTCTTCAACACAACCA+AGG | 0.687130 | 8.3:-68789331 | MS.gene051018:CDS |
| AAGTTTCCACCATGAAACCA+GGG | 0.691429 | 8.3:+68788640 | None:intergenic |
| AAAGTTTCTGAGAAAAGACA+AGG | 0.701580 | 8.3:-68788569 | MS.gene051018:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTGATAATATTATCTAA+TGG | - | chr8.3:68788827-68788846 | MS.gene051018:intron | 10.0% |
| !!! | TTAAACAAACTTGTTATTAA+AGG | + | chr8.3:68789235-68789254 | None:intergenic | 15.0% |
| !!! | TTTAGAGTTTTACAAAATCA+CGG | - | chr8.3:68788519-68788538 | MS.gene051018:CDS | 20.0% |
| !!! | TTTAGCTATAGATTTGTTAT+TGG | + | chr8.3:68789122-68789141 | None:intergenic | 20.0% |
| ! | TAAAACGAAAAATTGTACCA+GGG | + | chr8.3:68788443-68788462 | None:intergenic | 25.0% |
| ! | TTAAAACGAAAAATTGTACC+AGG | + | chr8.3:68788444-68788463 | None:intergenic | 25.0% |
| ! | TTAAAACTTCAACAAAACCA+CGG | + | chr8.3:68788720-68788739 | None:intergenic | 25.0% |
| !! | ACTAAATGCATTTGAAATCA+TGG | + | chr8.3:68788569-68788588 | None:intergenic | 25.0% |
| !! | TTTTGTGCAAGTTTGAATAA+CGG | - | chr8.3:68788669-68788688 | MS.gene051018:CDS | 25.0% |
| !!! | AGATTTTCACTCTTTGATTT+TGG | - | chr8.3:68788908-68788927 | MS.gene051018:intron | 25.0% |
| AAACATAGTTACTGCATGTT+TGG | - | chr8.3:68788462-68788481 | MS.gene051018:CDS | 30.0% | |
| GAAACTTGACAAGAAAAGTA+AGG | - | chr8.3:68789084-68789103 | MS.gene051018:intron | 30.0% | |
| GAAGAAGGATCTAAGAAAAA+AGG | - | chr8.3:68789004-68789023 | MS.gene051018:intron | 30.0% | |
| GTTATTAAAGGTCCAGAAAA+TGG | + | chr8.3:68789223-68789242 | None:intergenic | 30.0% | |
| ! | AAAGTTTCTGAGAAAAGACA+AGG | - | chr8.3:68789139-68789158 | MS.gene051018:intron | 30.0% |
| ! | AAGCAAAAGAATCCATTTTC+TGG | - | chr8.3:68789208-68789227 | MS.gene051018:intron | 30.0% |
| ! | TTTTCTTAGATCCTTCTTCA+GGG | + | chr8.3:68789003-68789022 | None:intergenic | 30.0% |
| ! | TTTTTCTTAGATCCTTCTTC+AGG | + | chr8.3:68789004-68789023 | None:intergenic | 30.0% |
| AAACTATGGTCCTCTTTATG+TGG | - | chr8.3:68789330-68789349 | MS.gene051018:CDS | 35.0% | |
| AACTCCTACAAAGGTTATGA+TGG | - | chr8.3:68789184-68789203 | MS.gene051018:intron | 35.0% | |
| ACAATTCTTCAACACAACCA+AGG | - | chr8.3:68788377-68788396 | MS.gene051018:CDS | 35.0% | |
| ACGGTGAATTTGACATAATC+AGG | + | chr8.3:68788776-68788795 | None:intergenic | 35.0% | |
| ACTCCTACAAAGGTTATGAT+GGG | - | chr8.3:68789185-68789204 | MS.gene051018:intron | 35.0% | |
| AGTTTGAATAACGGTGAGTT+TGG | - | chr8.3:68788678-68788697 | MS.gene051018:CDS | 35.0% | |
| CCAATACTTAACTCCTACAA+AGG | - | chr8.3:68789175-68789194 | MS.gene051018:intron | 35.0% | |
| CCTTTGTAGGAGTTAAGTAT+TGG | + | chr8.3:68789178-68789197 | None:intergenic | 35.0% | |
| CGGTGAATTTGACATAATCA+GGG | + | chr8.3:68788775-68788794 | None:intergenic | 35.0% | |
| TGAGAAAGGGAAAGAAATCA+TGG | - | chr8.3:68789038-68789057 | MS.gene051018:intron | 35.0% | |
| TTACTGCATGTTTGGATTCA+CGG | - | chr8.3:68788470-68788489 | MS.gene051018:CDS | 35.0% | |
| TTCGTGCATGTTTAGATTGA+CGG | + | chr8.3:68788795-68788814 | None:intergenic | 35.0% | |
| TTCTCTTCTGATCTCTTTGT+TGG | + | chr8.3:68789259-68789278 | None:intergenic | 35.0% | |
| ! | GATGAGGACTATTTTGTTGA+TGG | + | chr8.3:68789303-68789322 | None:intergenic | 35.0% |
| ! | TGTTGATGGAGAGTCATATA+AGG | + | chr8.3:68789289-68789308 | None:intergenic | 35.0% |
| !! | TAAGTATTGGACCAGAAACA+TGG | + | chr8.3:68789165-68789184 | None:intergenic | 35.0% |
| AAAAGACAAGGCCATGTTTC+TGG | - | chr8.3:68789151-68789170 | MS.gene051018:intron | 40.0% | |
| AAGTTTCCACCATGAAACCA+GGG | + | chr8.3:68789071-68789090 | None:intergenic | 40.0% | |
| CCATAGTCATCATAATGCCT+TGG | + | chr8.3:68788397-68788416 | None:intergenic | 40.0% | |
| CCTCATCCTGTTCAAAACTA+TGG | - | chr8.3:68789316-68789335 | MS.gene051018:CDS | 40.0% | |
| CTATGGATTTGATCTGCAAC+AGG | - | chr8.3:68788411-68788430 | MS.gene051018:CDS | 40.0% | |
| CTTCCCATCATAACCTTTGT+AGG | + | chr8.3:68789191-68789210 | None:intergenic | 40.0% | |
| GAAATCATGGAAAAGTTCCC+TGG | - | chr8.3:68789051-68789070 | MS.gene051018:intron | 40.0% | |
| GCAAAATCACAATAACACCG+TGG | - | chr8.3:68788700-68788719 | MS.gene051018:CDS | 40.0% | |
| TTTCCCTTTCTCACTTTCTC+TGG | + | chr8.3:68789031-68789050 | None:intergenic | 40.0% | |
| ! | CCAAGGCATTATGATGACTA+TGG | - | chr8.3:68788394-68788413 | MS.gene051018:CDS | 40.0% |
| !!! | AGAGGACCATAGTTTTGAAC+AGG | + | chr8.3:68789325-68789344 | None:intergenic | 40.0% |
| !!! | CCATAGTTTTGAACAGGATG+AGG | + | chr8.3:68789319-68789338 | None:intergenic | 40.0% |
| !!! | TTTTGGCTCTCACTAGTTTC+TGG | - | chr8.3:68788925-68788944 | MS.gene051018:intron | 40.0% |
| AAAGTTCCCTGGTTTCATGG+TGG | - | chr8.3:68789062-68789081 | MS.gene051018:intron | 45.0% | |
| AGGACCAGAGAAAGTGAGAA+AGG | - | chr8.3:68789024-68789043 | MS.gene051018:intron | 45.0% | |
| CAAGTTTCCACCATGAAACC+AGG | + | chr8.3:68789072-68789091 | None:intergenic | 45.0% | |
| GGAAAAGTTCCCTGGTTTCA+TGG | - | chr8.3:68789059-68789078 | MS.gene051018:intron | 45.0% | |
| GGACCAGAGAAAGTGAGAAA+GGG | - | chr8.3:68789025-68789044 | MS.gene051018:intron | 45.0% | |
| TCAACAGTGTACCCTGAAGA+AGG | - | chr8.3:68788989-68789008 | MS.gene051018:intron | 45.0% | |
| !! | CTAGTTTCTGGCTGAAGCAA+AGG | - | chr8.3:68788937-68788956 | MS.gene051018:intron | 45.0% |
| ! | TCTGCAACAGGACTTTTCCC+TGG | - | chr8.3:68788423-68788442 | MS.gene051018:CDS | 50.0% |
| CTGGCTGAAGCAAAGGAGCA+TGG | - | chr8.3:68788944-68788963 | MS.gene051018:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 68788370 | 68789360 | 68788370 | ID=MS.gene051018 |
| chr8.3 | mRNA | 68788370 | 68789360 | 68788370 | ID=MS.gene051018.t1;Parent=MS.gene051018 |
| chr8.3 | exon | 68789286 | 68789360 | 68789286 | ID=MS.gene051018.t1.exon1;Parent=MS.gene051018.t1 |
| chr8.3 | CDS | 68789286 | 68789360 | 68789286 | ID=cds.MS.gene051018.t1;Parent=MS.gene051018.t1 |
| chr8.3 | exon | 68788370 | 68788789 | 68788370 | ID=MS.gene051018.t1.exon2;Parent=MS.gene051018.t1 |
| chr8.3 | CDS | 68788370 | 68788789 | 68788370 | ID=cds.MS.gene051018.t1;Parent=MS.gene051018.t1 |
| Gene Sequence |
| Protein sequence |
