Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90480.t1 | XP_013457440.1 | 99.7 | 303 | 1 | 0 | 1 | 303 | 1 | 303 | 1.90E-171 | 611.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90480.t1 | Q9SNE5 | 65.1 | 312 | 95 | 4 | 1 | 298 | 1 | 312 | 7.8e-118 | 424.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90480.t1 | A0A072UQ49 | 99.7 | 303 | 1 | 0 | 1 | 303 | 1 | 303 | 1.3e-171 | 611.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049302 | MS.gene90480 | 0.816794 | 4.60E-52 | -1.69E-46 |
| MS.gene050649 | MS.gene90480 | 0.82709 | 1.90E-54 | -1.69E-46 |
| MS.gene058206 | MS.gene90480 | 0.830374 | 3.04E-55 | -1.69E-46 |
| MS.gene060317 | MS.gene90480 | 0.827221 | 1.76E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90480.t1 | MTR_4g094895 | 99.670 | 303 | 1 | 0 | 1 | 303 | 1 | 303 | 0.0 | 632 |
| MS.gene90480.t1 | MTR_4g485570 | 51.133 | 309 | 132 | 8 | 1 | 301 | 1 | 298 | 1.09e-93 | 280 |
| MS.gene90480.t1 | MTR_4g485560 | 57.328 | 232 | 93 | 4 | 74 | 302 | 21 | 249 | 2.83e-90 | 269 |
| MS.gene90480.t1 | MTR_8g080660 | 36.154 | 260 | 156 | 7 | 28 | 283 | 23 | 276 | 2.40e-53 | 176 |
| MS.gene90480.t1 | MTR_3g085270 | 35.206 | 267 | 165 | 6 | 34 | 296 | 24 | 286 | 2.19e-52 | 173 |
| MS.gene90480.t1 | MTR_3g005710 | 37.917 | 240 | 142 | 5 | 44 | 283 | 51 | 283 | 2.53e-51 | 171 |
| MS.gene90480.t1 | MTR_7g029100 | 28.472 | 288 | 179 | 8 | 14 | 296 | 14 | 279 | 8.29e-38 | 135 |
| MS.gene90480.t1 | MTR_4g102900 | 36.364 | 220 | 129 | 7 | 80 | 296 | 85 | 296 | 1.51e-36 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90480.t1 | AT5G67210 | 67.333 | 300 | 92 | 1 | 5 | 298 | 6 | 305 | 4.10e-162 | 454 |
| MS.gene90480.t1 | AT3G50220 | 64.744 | 312 | 96 | 3 | 1 | 298 | 1 | 312 | 3.15e-155 | 437 |
| MS.gene90480.t1 | AT2G15440 | 48.707 | 232 | 112 | 3 | 74 | 300 | 78 | 307 | 6.13e-75 | 233 |
| MS.gene90480.t1 | AT1G71690 | 37.024 | 289 | 163 | 9 | 23 | 296 | 10 | 294 | 9.71e-55 | 180 |
| MS.gene90480.t1 | AT1G33800 | 38.117 | 223 | 131 | 4 | 63 | 285 | 62 | 277 | 2.33e-48 | 163 |
| MS.gene90480.t1 | AT1G09610 | 33.846 | 260 | 165 | 5 | 33 | 292 | 20 | 272 | 1.37e-47 | 161 |
| MS.gene90480.t1 | AT1G67330 | 34.799 | 273 | 162 | 9 | 19 | 283 | 10 | 274 | 2.47e-45 | 155 |
| MS.gene90480.t1 | AT4G09990 | 36.122 | 263 | 153 | 7 | 29 | 283 | 13 | 268 | 2.28e-44 | 153 |
| MS.gene90480.t1 | AT1G27930 | 34.742 | 213 | 132 | 5 | 75 | 285 | 65 | 272 | 7.17e-42 | 146 |
| MS.gene90480.t1 | AT4G24910 | 37.915 | 211 | 114 | 8 | 95 | 296 | 105 | 307 | 1.73e-35 | 130 |
Find 56 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGAAGCAAGTGAGAGTA+TGG | 0.296973 | 8.3:-23375394 | MS.gene90480:CDS |
| AAGGAAACTCAAATCGATTA+TGG | 0.310137 | 8.3:-23376154 | MS.gene90480:CDS |
| GGTTGATTGGGATGTTATAT+TGG | 0.342392 | 8.3:-23375595 | MS.gene90480:CDS |
| GACATTCTCCCGGGAGCTTC+CGG | 0.344971 | 8.3:+23375549 | None:intergenic |
| GAGTATGGGGCATTATGTTT+TGG | 0.353571 | 8.3:-23375379 | MS.gene90480:CDS |
| AGAAATTATTGCAGTATTTG+TGG | 0.362131 | 8.3:+23376058 | None:intergenic |
| TTATTCTCGGATTGTAAACT+TGG | 0.373700 | 8.3:-23375648 | MS.gene90480:CDS |
| GGGGATTGGCCGGAAGCTCC+CGG | 0.374565 | 8.3:-23375558 | MS.gene90480:CDS |
| TGGAAAGCACTTAACCATAA+TGG | 0.380677 | 8.3:-23375843 | MS.gene90480:CDS |
| TCACACCAGAGACTCTTCTA+TGG | 0.388972 | 8.3:-23375863 | MS.gene90480:CDS |
| TTTCGAAGCACGTCGGAGAT+TGG | 0.397431 | 8.3:+23375921 | None:intergenic |
| ATGTTCAATATACAACAAAA+AGG | 0.412243 | 8.3:-23375743 | MS.gene90480:CDS |
| TGGGATGTTATATTGGTTGA+TGG | 0.413519 | 8.3:-23375588 | MS.gene90480:CDS |
| TTGATGGACCGCGCGGGGAT+TGG | 0.417573 | 8.3:-23375572 | MS.gene90480:CDS |
| TCAACCTCATACACATGGTT+TGG | 0.423157 | 8.3:+23375612 | None:intergenic |
| CAGTGTTCATCGATGAAAAT+CGG | 0.433830 | 8.3:-23375815 | MS.gene90480:CDS |
| ACATCATAAGCATCAATCTC+AGG | 0.451907 | 8.3:+23375762 | None:intergenic |
| GCTGGTAATGGTGCAGAAAC+AGG | 0.455046 | 8.3:+23376020 | None:intergenic |
| AAATATCGCCGACATTCTCC+CGG | 0.466806 | 8.3:+23375539 | None:intergenic |
| ACCATGTGTATGAGGTTGAT+TGG | 0.473095 | 8.3:-23375608 | MS.gene90480:CDS |
| GCTTTCCATAGAAGAGTCTC+TGG | 0.473937 | 8.3:+23375858 | None:intergenic |
| TTGATAACTGTTGCTGGTAA+TGG | 0.483594 | 8.3:+23376008 | None:intergenic |
| AGCAGTGTTCAGTATTGTAA+AGG | 0.486973 | 8.3:-23375342 | MS.gene90480:CDS |
| CCATGTGTATGAGGTTGATT+GGG | 0.495004 | 8.3:-23375607 | MS.gene90480:CDS |
| TGGACCGCGCGGGGATTGGC+CGG | 0.499438 | 8.3:-23375568 | MS.gene90480:CDS |
| GGGATTGGCCGGAAGCTCCC+GGG | 0.501763 | 8.3:-23375557 | MS.gene90480:CDS |
| AGAGTGTTGATAACTGTTGC+TGG | 0.504927 | 8.3:+23376002 | None:intergenic |
| TATTGGTTGATGGACCGCGC+GGG | 0.516968 | 8.3:-23375578 | MS.gene90480:CDS |
| GCAGTGTTCAGTATTGTAAA+GGG | 0.522073 | 8.3:-23375341 | MS.gene90480:CDS |
| GGTGAAGTTGAACAAGTTTG+TGG | 0.522331 | 8.3:-23375444 | MS.gene90480:CDS |
| TTTATGTAAAGAGAATTTGT+TGG | 0.541273 | 8.3:-23375412 | MS.gene90480:CDS |
| GATATTTACCGCCGGTGTGT+TGG | 0.542500 | 8.3:-23375523 | MS.gene90480:CDS |
| TCTTCCAAACCATGTGTATG+AGG | 0.545799 | 8.3:-23375616 | MS.gene90480:CDS |
| ATTGGTTGATGGACCGCGCG+GGG | 0.550084 | 8.3:-23375577 | MS.gene90480:CDS |
| GAGAATAAAAGATTCTGCAC+TGG | 0.555559 | 8.3:+23375663 | None:intergenic |
| TTGGAAGCAAGTGAGAGTAT+GGG | 0.563393 | 8.3:-23375393 | MS.gene90480:CDS |
| AATATCGCCGACATTCTCCC+GGG | 0.567547 | 8.3:+23375540 | None:intergenic |
| GTGAAGTTGAACAAGTTTGT+GGG | 0.568240 | 8.3:-23375443 | MS.gene90480:CDS |
| TGTCATGTGGTATGAGTCGT+TGG | 0.578039 | 8.3:+23375959 | None:intergenic |
| GATTTCAAAGATGCATTAGG+TGG | 0.578147 | 8.3:+23375308 | None:intergenic |
| GGAAGCTCCCGGGAGAATGT+CGG | 0.592858 | 8.3:-23375547 | MS.gene90480:CDS |
| GTGTTGGCTAGAAGTAAGAA+AGG | 0.593440 | 8.3:-23375507 | MS.gene90480:CDS |
| CCCAATCAACCTCATACACA+TGG | 0.608735 | 8.3:+23375607 | None:intergenic |
| CATCATAAGCATCAATCTCA+GGG | 0.612180 | 8.3:+23375763 | None:intergenic |
| TGGAAGCAAGTGAGAGTATG+GGG | 0.630068 | 8.3:-23375392 | MS.gene90480:CDS |
| ATAGAAGAGTCTCTGGTGTG+AGG | 0.646087 | 8.3:+23375865 | None:intergenic |
| TTGGCTAGAAGTAAGAAAGG+TGG | 0.656828 | 8.3:-23375504 | MS.gene90480:CDS |
| ATGTCGGCGATATTTACCGC+CGG | 0.657606 | 8.3:-23375531 | MS.gene90480:CDS |
| CTTACTTCTAGCCAACACAC+CGG | 0.667962 | 8.3:+23375512 | None:intergenic |
| TTGTGTAAATGAGTGTTGCA+AGG | 0.669621 | 8.3:+23376096 | None:intergenic |
| TGAACACTTTCGAAGCACGT+CGG | 0.688069 | 8.3:+23375914 | None:intergenic |
| ACTTCTAGCCAACACACCGG+CGG | 0.696649 | 8.3:+23375515 | None:intergenic |
| TAATGGTGCAGAAACAGGAG+TGG | 0.701846 | 8.3:+23376025 | None:intergenic |
| TGAGATCAGAGTATGTCATG+TGG | 0.715244 | 8.3:+23375946 | None:intergenic |
| ATATTGGTTGATGGACCGCG+CGG | 0.745881 | 8.3:-23375579 | MS.gene90480:CDS |
| GCTTCCGGCCAATCCCCGCG+CGG | 0.746128 | 8.3:+23375564 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTCAATATACAACAAAA+AGG | - | chr8.3:23375792-23375811 | MS.gene90480:CDS | 20.0% |
| !! | TTTATGTAAAGAGAATTTGT+TGG | - | chr8.3:23376123-23376142 | MS.gene90480:CDS | 20.0% |
| ! | AGAAATTATTGCAGTATTTG+TGG | + | chr8.3:23375480-23375499 | None:intergenic | 25.0% |
| !!! | GTTTTTTTGCATGATTTTTC+TGG | - | chr8.3:23376070-23376089 | MS.gene90480:CDS | 25.0% |
| !!! | TTTCCTTGTTTTTGGATATA+TGG | + | chr8.3:23375368-23375387 | None:intergenic | 25.0% |
| AAGGAAACTCAAATCGATTA+TGG | - | chr8.3:23375381-23375400 | MS.gene90480:CDS | 30.0% | |
| CATCCATATATCCAAAAACA+AGG | - | chr8.3:23375362-23375381 | MS.gene90480:CDS | 30.0% | |
| CCAAAAATAAGCAAGTTACA+TGG | + | chr8.3:23375650-23375669 | None:intergenic | 30.0% | |
| GCAAAAAAACATGAGTCTTT+GGG | + | chr8.3:23376060-23376079 | None:intergenic | 30.0% | |
| TGCAAAAAAACATGAGTCTT+TGG | + | chr8.3:23376061-23376080 | None:intergenic | 30.0% | |
| TTATTCTCGGATTGTAAACT+TGG | - | chr8.3:23375887-23375906 | MS.gene90480:CDS | 30.0% | |
| ! | AGTGCAGAATCTTTTATTCT+CGG | - | chr8.3:23375874-23375893 | MS.gene90480:CDS | 30.0% |
| !! | AAGAAGAATGAGTTTTGTGT+TGG | + | chr8.3:23375342-23375361 | None:intergenic | 30.0% |
| !! | TGAACACTGTTTTTCCATTA+TGG | + | chr8.3:23375709-23375728 | None:intergenic | 30.0% |
| !!! | CCATGTAACTTGCTTATTTT+TGG | - | chr8.3:23375647-23375666 | MS.gene90480:CDS | 30.0% |
| !!! | GATTTGAGTTTCCTTGTTTT+TGG | + | chr8.3:23375376-23375395 | None:intergenic | 30.0% |
| ACATCATAAGCATCAATCTC+AGG | + | chr8.3:23375776-23375795 | None:intergenic | 35.0% | |
| AGCAGTGTTCAGTATTGTAA+AGG | - | chr8.3:23376193-23376212 | MS.gene90480:CDS | 35.0% | |
| CAGTGTTCATCGATGAAAAT+CGG | - | chr8.3:23375720-23375739 | MS.gene90480:CDS | 35.0% | |
| CATCATAAGCATCAATCTCA+GGG | + | chr8.3:23375775-23375794 | None:intergenic | 35.0% | |
| GAGAATAAAAGATTCTGCAC+TGG | + | chr8.3:23375875-23375894 | None:intergenic | 35.0% | |
| GCAGTGTTCAGTATTGTAAA+GGG | - | chr8.3:23376194-23376213 | MS.gene90480:CDS | 35.0% | |
| GGTTGATTGGGATGTTATAT+TGG | - | chr8.3:23375940-23375959 | MS.gene90480:CDS | 35.0% | |
| GTGAAGTTGAACAAGTTTGT+GGG | - | chr8.3:23376092-23376111 | MS.gene90480:CDS | 35.0% | |
| ! | TGGGATGTTATATTGGTTGA+TGG | - | chr8.3:23375947-23375966 | MS.gene90480:CDS | 35.0% |
| ! | TTGATAACTGTTGCTGGTAA+TGG | + | chr8.3:23375530-23375549 | None:intergenic | 35.0% |
| ! | TTGTGTAAATGAGTGTTGCA+AGG | + | chr8.3:23375442-23375461 | None:intergenic | 35.0% |
| !! | TGGAAAGCACTTAACCATAA+TGG | - | chr8.3:23375692-23375711 | MS.gene90480:CDS | 35.0% |
| !!! | TGGATTTTGAAGCATAGTGA+AGG | + | chr8.3:23375559-23375578 | None:intergenic | 35.0% |
| GGTGAAGTTGAACAAGTTTG+TGG | - | chr8.3:23376091-23376110 | MS.gene90480:CDS | 40.0% | |
| TCAACCTCATACACATGGTT+TGG | + | chr8.3:23375926-23375945 | None:intergenic | 40.0% | |
| TCTTCCAAACCATGTGTATG+AGG | - | chr8.3:23375919-23375938 | MS.gene90480:CDS | 40.0% | |
| TGAGATCAGAGTATGTCATG+TGG | + | chr8.3:23375592-23375611 | None:intergenic | 40.0% | |
| TTGGCTAGAAGTAAGAAAGG+TGG | - | chr8.3:23376031-23376050 | MS.gene90480:CDS | 40.0% | |
| ! | ACCATGTGTATGAGGTTGAT+TGG | - | chr8.3:23375927-23375946 | MS.gene90480:CDS | 40.0% |
| ! | CCATGTGTATGAGGTTGATT+GGG | - | chr8.3:23375928-23375947 | MS.gene90480:CDS | 40.0% |
| ! | TTGGAAGCAAGTGAGAGTAT+GGG | - | chr8.3:23376142-23376161 | MS.gene90480:CDS | 40.0% |
| !! | AGAGTGTTGATAACTGTTGC+TGG | + | chr8.3:23375536-23375555 | None:intergenic | 40.0% |
| !! | GAGTATGGGGCATTATGTTT+TGG | - | chr8.3:23376156-23376175 | MS.gene90480:CDS | 40.0% |
| !! | GTGTTGGCTAGAAGTAAGAA+AGG | - | chr8.3:23376028-23376047 | MS.gene90480:CDS | 40.0% |
| !!! | TGGGGCATTATGTTTTGGAA+AGG | - | chr8.3:23376161-23376180 | MS.gene90480:CDS | 40.0% |
| AAATATCGCCGACATTCTCC+CGG | + | chr8.3:23375999-23376018 | None:intergenic | 45.0% | |
| ATAGAAGAGTCTCTGGTGTG+AGG | + | chr8.3:23375673-23375692 | None:intergenic | 45.0% | |
| CCCAATCAACCTCATACACA+TGG | + | chr8.3:23375931-23375950 | None:intergenic | 45.0% | |
| CTTACTTCTAGCCAACACAC+CGG | + | chr8.3:23376026-23376045 | None:intergenic | 45.0% | |
| GCTTTCCATAGAAGAGTCTC+TGG | + | chr8.3:23375680-23375699 | None:intergenic | 45.0% | |
| GTTGGAAGCAAGTGAGAGTA+TGG | - | chr8.3:23376141-23376160 | MS.gene90480:CDS | 45.0% | |
| TAATGGTGCAGAAACAGGAG+TGG | + | chr8.3:23375513-23375532 | None:intergenic | 45.0% | |
| TCACACCAGAGACTCTTCTA+TGG | - | chr8.3:23375672-23375691 | MS.gene90480:CDS | 45.0% | |
| TGAACACTTTCGAAGCACGT+CGG | + | chr8.3:23375624-23375643 | None:intergenic | 45.0% | |
| TGTCATGTGGTATGAGTCGT+TGG | + | chr8.3:23375579-23375598 | None:intergenic | 45.0% | |
| ! | TGGAAGCAAGTGAGAGTATG+GGG | - | chr8.3:23376143-23376162 | MS.gene90480:CDS | 45.0% |
| AATATCGCCGACATTCTCCC+GGG | + | chr8.3:23375998-23376017 | None:intergenic | 50.0% | |
| ATGTCGGCGATATTTACCGC+CGG | - | chr8.3:23376004-23376023 | MS.gene90480:CDS | 50.0% | |
| GATATTTACCGCCGGTGTGT+TGG | - | chr8.3:23376012-23376031 | MS.gene90480:CDS | 50.0% | |
| TTTCGAAGCACGTCGGAGAT+TGG | + | chr8.3:23375617-23375636 | None:intergenic | 50.0% | |
| !! | ATATTGGTTGATGGACCGCG+CGG | - | chr8.3:23375956-23375975 | MS.gene90480:CDS | 50.0% |
| !! | GCTGGTAATGGTGCAGAAAC+AGG | + | chr8.3:23375518-23375537 | None:intergenic | 50.0% |
| ACTTCTAGCCAACACACCGG+CGG | + | chr8.3:23376023-23376042 | None:intergenic | 55.0% | |
| !! | TATTGGTTGATGGACCGCGC+GGG | - | chr8.3:23375957-23375976 | MS.gene90480:CDS | 55.0% |
| GACATTCTCCCGGGAGCTTC+CGG | + | chr8.3:23375989-23376008 | None:intergenic | 60.0% | |
| GGAAGCTCCCGGGAGAATGT+CGG | - | chr8.3:23375988-23376007 | MS.gene90480:CDS | 60.0% | |
| !! | ATTGGTTGATGGACCGCGCG+GGG | - | chr8.3:23375958-23375977 | MS.gene90480:CDS | 60.0% |
| ! | TTGATGGACCGCGCGGGGAT+TGG | - | chr8.3:23375963-23375982 | MS.gene90480:CDS | 65.0% |
| !! | GGGATTGGCCGGAAGCTCCC+GGG | - | chr8.3:23375978-23375997 | MS.gene90480:CDS | 70.0% |
| !! | GGGGATTGGCCGGAAGCTCC+CGG | - | chr8.3:23375977-23375996 | MS.gene90480:CDS | 70.0% |
| GCTTCCGGCCAATCCCCGCG+CGG | + | chr8.3:23375974-23375993 | None:intergenic | 75.0% | |
| ! | TGGACCGCGCGGGGATTGGC+CGG | - | chr8.3:23375967-23375986 | MS.gene90480:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 23375323 | 23376234 | 23375323 | ID=MS.gene90480 |
| chr8.3 | mRNA | 23375323 | 23376234 | 23375323 | ID=MS.gene90480.t1;Parent=MS.gene90480 |
| chr8.3 | exon | 23375323 | 23376234 | 23375323 | ID=MS.gene90480.t1.exon1;Parent=MS.gene90480.t1 |
| chr8.3 | CDS | 23375323 | 23376234 | 23375323 | ID=cds.MS.gene90480.t1;Parent=MS.gene90480.t1 |
| Gene Sequence |
| Protein sequence |
