AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91475

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91475.t1 MTR_7g035425 32.500 120 75 2 55 168 217 336 1.14e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 91 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATAATAGCATCTTTATT+AGG 0.143812 6.1:+4701272 None:intergenic
GGCTATTAGGCGTCAATTTC+GGG 0.176665 6.1:-4701093 MS.gene91475:CDS
GAGAGAAACGACTTCAATTA+TGG 0.254171 6.1:-4699865 MS.gene91475:CDS
TGGCTATTAGGCGTCAATTT+CGG 0.262175 6.1:-4701094 MS.gene91475:CDS
TTATAATAGCATCTTTATTA+GGG 0.266058 6.1:+4701273 None:intergenic
GCTTACAAAAGTAAGAAATA+AGG 0.277692 6.1:-4700818 MS.gene91475:CDS
ATGCTGGTCTTCTTGAATAT+TGG 0.281800 6.1:-4700762 MS.gene91475:CDS
CCATCCTGATGAACTCTTCT+TGG 0.306942 6.1:-4700512 MS.gene91475:CDS
GCATCCTCTCCCATCCATTT+AGG 0.314255 6.1:+4700787 None:intergenic
CTTCACAAAACAAGACTAAT+CGG 0.320914 6.1:-4700714 MS.gene91475:CDS
ATAAAATACCCTTGACCAAT+TGG 0.342640 6.1:+4701236 None:intergenic
GATCAGCTTCAGCAGCTCTT+TGG 0.351605 6.1:+4699666 None:intergenic
AAGATGTTCCTCAAGATCTA+TGG 0.363798 6.1:-4701052 MS.gene91475:CDS
CTACTGCTATGGAAGAATTC+AGG 0.367453 6.1:-4699613 MS.gene91475:CDS
ATGCAATGAGGATGGCTATT+AGG 0.367943 6.1:-4701106 MS.gene91475:CDS
CTCAATATGATACGGAAATC+AGG 0.372504 6.1:-4700882 MS.gene91475:CDS
GCATGTCAGTAAGGCGCTTT+GGG 0.375371 6.1:+4700839 None:intergenic
ATAAGGGTGAAAGACCTAAA+TGG 0.377120 6.1:-4700801 MS.gene91475:CDS
CAATTATGGGTAGATGTTGC+TGG 0.379900 6.1:-4699851 MS.gene91475:CDS
AGCATGTCAGTAAGGCGCTT+TGG 0.396055 6.1:+4700838 None:intergenic
AACAAGACTAATCGGGGTTC+GGG 0.400300 6.1:-4700706 MS.gene91475:CDS
AATTATGGGTAGATGTTGCT+GGG 0.404667 6.1:-4699850 MS.gene91475:CDS
CAATCTTTGTTAGTTGAGTC+AGG 0.404969 6.1:+4699924 None:intergenic
GACTGACAGTGTAAAATACT+TGG 0.426930 6.1:-4700544 MS.gene91475:intron
CTTACAAAAGTAAGAAATAA+GGG 0.428499 6.1:-4700817 MS.gene91475:CDS
TTCACAAAACAAGACTAATC+GGG 0.432488 6.1:-4700713 MS.gene91475:CDS
AAACAAGACTAATCGGGGTT+CGG 0.436178 6.1:-4700707 MS.gene91475:CDS
AAGATTGATGAGAATTCTAA+TGG 0.441022 6.1:-4699908 MS.gene91475:CDS
TCTGGCAGGAATATGTTTCA+TGG 0.456255 6.1:-4700909 MS.gene91475:intron
GGCTGCAGCAGCACGTGCTG+AGG 0.459336 6.1:-4699702 MS.gene91475:CDS
ACTAATCGGGGTTCGGGCAA+AGG 0.464856 6.1:-4700700 MS.gene91475:CDS
ACGTGCTGAGGCTGCTGCAG+CGG 0.467695 6.1:-4699690 MS.gene91475:CDS
TAAGAGGAAGTCTGGCGATT+GGG 0.469252 6.1:-4700482 MS.gene91475:CDS
AGAGAAACGACTTCAATTAT+GGG 0.470876 6.1:-4699864 MS.gene91475:CDS
GTAGCCAAGAAGAGTTCATC+AGG 0.473619 6.1:+4700508 None:intergenic
GGTGATTGAAATGTTTCGTC+AGG 0.476769 6.1:-4699726 MS.gene91475:CDS
TACCTTTGAACGAGCATTGG+TGG 0.478671 6.1:+4701682 None:intergenic
TAAAATACCCTTGACCAATT+GGG 0.480390 6.1:+4701237 None:intergenic
TCTTCTTGGCTACTCGTAAG+AGG 0.493331 6.1:-4700498 MS.gene91475:CDS
TAATACCTTTGAACGAGCAT+TGG 0.494955 6.1:+4701679 None:intergenic
GCTACTCGTAAGAGGAAGTC+TGG 0.496029 6.1:-4700490 MS.gene91475:CDS
AAGACCATCATACGCCCAAT+TGG 0.499185 6.1:-4701251 MS.gene91475:CDS
GCATCTTTATTAGGGGACTC+TGG 0.512229 6.1:+4701281 None:intergenic
GCATTAGCACCATCTTTGCT+AGG 0.519763 6.1:+4701125 None:intergenic
GAATCAATTCTCTACTGCTA+TGG 0.520159 6.1:-4699624 MS.gene91475:CDS
AGATTTACTCCTAGCAAAGA+TGG 0.521609 6.1:-4701134 MS.gene91475:CDS
GAAGCTTTAGAGAATCAATC+AGG 0.522988 6.1:-4699581 MS.gene91475:CDS
TTGACCAATTGGGCGTATGA+TGG 0.526388 6.1:+4701247 None:intergenic
TGTCTATACCACAGGTCGTA+AGG 0.535312 6.1:-4700671 MS.gene91475:CDS
TTTATTAGGGGACTCTGGCT+CGG 0.535766 6.1:+4701286 None:intergenic
CTGGCAGGAATATGTTTCAT+GGG 0.543960 6.1:-4700908 MS.gene91475:intron
AAGAGTTCATCAGGATGGAT+AGG 0.544009 6.1:+4700517 None:intergenic
GTAAGAGGAAGTCTGGCGAT+TGG 0.544893 6.1:-4700483 MS.gene91475:CDS
GGGTGAAAGACCTAAATGGA+TGG 0.549625 6.1:-4700797 MS.gene91475:CDS
ATCATACGCCCAATTGGTCA+AGG 0.549638 6.1:-4701245 MS.gene91475:CDS
ATTTCAAACCATAGATCTTG+AGG 0.552511 6.1:+4701044 None:intergenic
TCTGATTGATCATCAAGAGC+AGG 0.560143 6.1:+4699503 None:intergenic
TTCCACCAATGCTCGTTCAA+AGG 0.564669 6.1:-4701684 MS.gene91475:intron
AATCGGGGTTCGGGCAAAGG+AGG 0.570234 6.1:-4700697 MS.gene91475:CDS
ATGGGAGACTCAATATGATA+CGG 0.573075 6.1:-4700890 MS.gene91475:CDS
CCATGACGTGGCACTAGACA+TGG 0.574192 6.1:-4700644 MS.gene91475:intron
ATGCCGTCCTCCTGAAAATG+AGG 0.579452 6.1:-4699747 MS.gene91475:CDS
CCAAGAAGAGTTCATCAGGA+TGG 0.580522 6.1:+4700512 None:intergenic
GGAAGAATTCAGGAAGCGCA+TGG 0.584480 6.1:-4699603 MS.gene91475:CDS
GATTGAAATGTTTCGTCAGG+AGG 0.588208 6.1:-4699723 MS.gene91475:CDS
TATAATAGCATCTTTATTAG+GGG 0.588230 6.1:+4701274 None:intergenic
TTAGAGAATCAATCAGGCGG+TGG 0.588913 6.1:-4699575 MS.gene91475:CDS
TAATGGTGATATGCAAGAGG+TGG 0.590246 6.1:-4699891 MS.gene91475:CDS
ATGGGAGAGGATGCATATGC+TGG 0.593184 6.1:-4700778 MS.gene91475:CDS
GATTGGGTTGACAGTCGTGC+TGG 0.597801 6.1:-4700466 MS.gene91475:CDS
TGCTCTTGATGATCAATCAG+AGG 0.598343 6.1:-4699501 MS.gene91475:CDS
ATTATGGGTAGATGTTGCTG+GGG 0.600229 6.1:-4699849 MS.gene91475:CDS
GGAGGAGCTGTCTATACCAC+AGG 0.602141 6.1:-4700679 MS.gene91475:CDS
ACTGACAGTGTAAAATACTT+GGG 0.608198 6.1:-4700543 MS.gene91475:intron
ATGGTGAGCCTTACGACCTG+TGG 0.611657 6.1:+4700663 None:intergenic
AAAATGACTAGACAATGTCA+TGG 0.611773 6.1:-4701725 MS.gene91475:CDS
CCATGTCTAGTGCCACGTCA+TGG 0.614191 6.1:+4700644 None:intergenic
TTATGGGTAGATGTTGCTGG+GGG 0.616713 6.1:-4699848 MS.gene91475:intron
AAGACCTAAATGGATGGGAG+AGG 0.617037 6.1:-4700791 MS.gene91475:CDS
GGTGAAAGACCTAAATGGAT+GGG 0.618316 6.1:-4700796 MS.gene91475:CDS
AATGGTGATATGCAAGAGGT+GGG 0.621480 6.1:-4699890 MS.gene91475:CDS
TCACAAAACAAGACTAATCG+GGG 0.623382 6.1:-4700712 MS.gene91475:CDS
TTCTAATGGTGATATGCAAG+AGG 0.631960 6.1:-4699894 MS.gene91475:CDS
TCAAGAGCAGGATCATAATG+TGG 0.646262 6.1:+4699515 None:intergenic
TCATACGCCCAATTGGTCAA+GGG 0.653662 6.1:-4701244 MS.gene91475:intron
TGGTGCTAATGCAATGAGGA+TGG 0.662918 6.1:-4701114 MS.gene91475:CDS
ACACCAACAAACATTAGGTG+AGG 0.663720 6.1:+4699474 None:intergenic
GGTGATATGCAAGAGGTGGG+CGG 0.675105 6.1:-4699887 MS.gene91475:CDS
TCGTAAGGCTCACCATGACG+TGG 0.697314 6.1:-4700656 MS.gene91475:CDS
GCTTTAGAGAATCAATCAGG+CGG 0.724831 6.1:-4699578 MS.gene91475:CDS
AAGATGGTGCTAATGCAATG+AGG 0.735156 6.1:-4701118 MS.gene91475:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGATCAAAATACATATA+TGG - chr6.1:4701001-4701020 MS.gene91475:intron 15.0%
!! TTATAATAGCATCTTTATTA+GGG + chr6.1:4699939-4699958 None:intergenic 15.0%
!! TTTATAATAGCATCTTTATT+AGG + chr6.1:4699940-4699959 None:intergenic 15.0%
!! AGATATTAAAGAAATTGGAA+TGG + chr6.1:4700598-4700617 None:intergenic 20.0%
!! ATATGTGAACACATATAATT+TGG - chr6.1:4701111-4701130 MS.gene91475:CDS 20.0%
!! ATATGTGAACATATATAATC+TGG - chr6.1:4700887-4700906 MS.gene91475:CDS 20.0%
!! CTTACAAAAGTAAGAAATAA+GGG - chr6.1:4700392-4700411 MS.gene91475:intron 20.0%
!! GAACTAGATATTAAAGAAAT+TGG + chr6.1:4700603-4700622 None:intergenic 20.0%
!! TATAATAGCATCTTTATTAG+GGG + chr6.1:4699938-4699957 None:intergenic 20.0%
!!! AATAAGTTGTGTTTTTTTAC+AGG - chr6.1:4701246-4701265 MS.gene91475:CDS 20.0%
!!! AGACATTACTATTTGTTTTA+TGG - chr6.1:4700639-4700658 MS.gene91475:intron 20.0%
!!! TCTATGGTTTGAAATATTTT+CGG - chr6.1:4700173-4700192 MS.gene91475:intron 20.0%
!!! TTTGCTATTTTTATATTGTC+TGG - chr6.1:4700282-4700301 MS.gene91475:intron 20.0%
! AAGATTGATGAGAATTCTAA+TGG - chr6.1:4701301-4701320 MS.gene91475:CDS 25.0%
! GCTTACAAAAGTAAGAAATA+AGG - chr6.1:4700391-4700410 MS.gene91475:intron 25.0%
AAAGAATAGTGTAGCTTCAT+TGG - chr6.1:4700206-4700225 MS.gene91475:intron 30.0%
ACTGACAGTGTAAAATACTT+GGG - chr6.1:4700666-4700685 MS.gene91475:CDS 30.0%
AGAGAAACGACTTCAATTAT+GGG - chr6.1:4701345-4701364 MS.gene91475:intron 30.0%
ATAAAATACCCTTGACCAAT+TGG + chr6.1:4699976-4699995 None:intergenic 30.0%
ATGGATAAGTTATGTAAACC+TGG - chr6.1:4701020-4701039 MS.gene91475:intron 30.0%
ATTTCAAACCATAGATCTTG+AGG + chr6.1:4700168-4700187 None:intergenic 30.0%
CACAAGCACATAATGAATTA+TGG + chr6.1:4700930-4700949 None:intergenic 30.0%
CTTCACAAAACAAGACTAAT+CGG - chr6.1:4700495-4700514 MS.gene91475:CDS 30.0%
TAAAATACCCTTGACCAATT+GGG + chr6.1:4699975-4699994 None:intergenic 30.0%
TAACACATATCTCAAGATTG+TGG + chr6.1:4701087-4701106 None:intergenic 30.0%
TCTCAAATTGCAAATATTCC+AGG + chr6.1:4701041-4701060 None:intergenic 30.0%
TTATCTTTCAACATTAGACC+AGG - chr6.1:4701418-4701437 MS.gene91475:intron 30.0%
TTCACAAAACAAGACTAATC+GGG - chr6.1:4700496-4700515 MS.gene91475:CDS 30.0%
TTGTCTTGAGGTAATTGTTA+TGG - chr6.1:4701385-4701404 MS.gene91475:intron 30.0%
! ATTTCAATCACCTCATTTTC+AGG + chr6.1:4701475-4701494 None:intergenic 30.0%
! CAGAAGCACATAATGAATTA+TGG + chr6.1:4701154-4701173 None:intergenic 30.0%
!! GGTTTGAAATATTTTCGGTA+AGG - chr6.1:4700178-4700197 MS.gene91475:intron 30.0%
!!! CTATTTTTATATTGTCTGGC+AGG - chr6.1:4700286-4700305 MS.gene91475:intron 30.0%
AAGATGTTCCTCAAGATCTA+TGG - chr6.1:4700157-4700176 MS.gene91475:intron 35.0%
AATTATGGGTAGATGTTGCT+GGG - chr6.1:4701359-4701378 MS.gene91475:intron 35.0%
AGATTTACTCCTAGCAAAGA+TGG - chr6.1:4700075-4700094 MS.gene91475:intron 35.0%
AGGTAATTGTTATGGTACTG+GGG - chr6.1:4701393-4701412 MS.gene91475:intron 35.0%
ATAAGGGTGAAAGACCTAAA+TGG - chr6.1:4700408-4700427 MS.gene91475:intron 35.0%
ATGGGAGACTCAATATGATA+CGG - chr6.1:4700319-4700338 MS.gene91475:intron 35.0%
CAATCTTTGTTAGTTGAGTC+AGG + chr6.1:4701288-4701307 None:intergenic 35.0%
CTCAAGATTGTGGACATATT+CGG + chr6.1:4701077-4701096 None:intergenic 35.0%
CTCAATATGATACGGAAATC+AGG - chr6.1:4700327-4700346 MS.gene91475:intron 35.0%
GAATCAATTCTCTACTGCTA+TGG - chr6.1:4701585-4701604 MS.gene91475:intron 35.0%
GACTGACAGTGTAAAATACT+TGG - chr6.1:4700665-4700684 MS.gene91475:CDS 35.0%
GAGAGAAACGACTTCAATTA+TGG - chr6.1:4701344-4701363 MS.gene91475:intron 35.0%
GAGGTAATTGTTATGGTACT+GGG - chr6.1:4701392-4701411 MS.gene91475:intron 35.0%
TAATACCTTTGAACGAGCAT+TGG + chr6.1:4699533-4699552 None:intergenic 35.0%
TCACAAAACAAGACTAATCG+GGG - chr6.1:4700497-4700516 MS.gene91475:CDS 35.0%
TGAGGTAATTGTTATGGTAC+TGG - chr6.1:4701391-4701410 MS.gene91475:intron 35.0%
TTCTAATGGTGATATGCAAG+AGG - chr6.1:4701315-4701334 MS.gene91475:intron 35.0%
! CTTTTGTAAGCATGTCAGTA+AGG + chr6.1:4700382-4700401 None:intergenic 35.0%
!! ATGCTGGTCTTCTTGAATAT+TGG - chr6.1:4700447-4700466 MS.gene91475:intron 35.0%
!! GAAGCTTTAGAGAATCAATC+AGG - chr6.1:4701628-4701647 MS.gene91475:intron 35.0%
AAACAAGACTAATCGGGGTT+CGG - chr6.1:4700502-4700521 MS.gene91475:CDS 40.0%
AAGAGTTCATCAGGATGGAT+AGG + chr6.1:4700695-4700714 None:intergenic 40.0%
AATCCACAAGACATCAACTG+TGG - chr6.1:4699657-4699676 MS.gene91475:CDS 40.0%
AATGGTGATATGCAAGAGGT+GGG - chr6.1:4701319-4701338 MS.gene91475:intron 40.0%
ATGCAATGAGGATGGCTATT+AGG - chr6.1:4700103-4700122 MS.gene91475:intron 40.0%
ATTATGGGTAGATGTTGCTG+GGG - chr6.1:4701360-4701379 MS.gene91475:intron 40.0%
CAATTATGGGTAGATGTTGC+TGG - chr6.1:4701358-4701377 MS.gene91475:intron 40.0%
CTACTGCTATGGAAGAATTC+AGG - chr6.1:4701596-4701615 MS.gene91475:intron 40.0%
CTGGCAGGAATATGTTTCAT+GGG - chr6.1:4700301-4700320 MS.gene91475:intron 40.0%
GAAAAAAACTGGGGTTACCA+CGG - chr6.1:4699611-4699630 MS.gene91475:CDS 40.0%
GATATCTTGCTAAAGCAACC+AGG - chr6.1:4699560-4699579 MS.gene91475:CDS 40.0%
GATTGAAATGTTTCGTCAGG+AGG - chr6.1:4701486-4701505 MS.gene91475:intron 40.0%
GGTGAAAGACCTAAATGGAT+GGG - chr6.1:4700413-4700432 MS.gene91475:intron 40.0%
GGTGATTGAAATGTTTCGTC+AGG - chr6.1:4701483-4701502 MS.gene91475:intron 40.0%
TAATGGTGATATGCAAGAGG+TGG - chr6.1:4701318-4701337 MS.gene91475:intron 40.0%
TCAAGAGCAGGATCATAATG+TGG + chr6.1:4701697-4701716 None:intergenic 40.0%
TCTGATTGATCATCAAGAGC+AGG + chr6.1:4701709-4701728 None:intergenic 40.0%
TCTGGCAGGAATATGTTTCA+TGG - chr6.1:4700300-4700319 MS.gene91475:intron 40.0%
TGAGTTAGATGACTTACACC+TGG + chr6.1:4701439-4701458 None:intergenic 40.0%
TGGCTATTAGGCGTCAATTT+CGG - chr6.1:4700115-4700134 MS.gene91475:intron 40.0%
! AAGATGGTGCTAATGCAATG+AGG - chr6.1:4700091-4700110 MS.gene91475:intron 40.0%
! CATATTTCTGCAGCAACCAA+AGG + chr6.1:4700254-4700273 None:intergenic 40.0%
! GCTTCATTGGTTGAAAACTG+TGG - chr6.1:4700219-4700238 MS.gene91475:intron 40.0%
! GTTGATGTCTTGTGGATTGT+TGG + chr6.1:4699655-4699674 None:intergenic 40.0%
! GTTGCTTTAGCAAGATATCG+TGG + chr6.1:4699559-4699578 None:intergenic 40.0%
! TGCTCTTGATGATCAATCAG+AGG - chr6.1:4701708-4701727 MS.gene91475:CDS 40.0%
!! GCTTTAGAGAATCAATCAGG+CGG - chr6.1:4701631-4701650 MS.gene91475:intron 40.0%
!! TCAATCACCTCATTTTCAGG+AGG + chr6.1:4701472-4701491 None:intergenic 40.0%
!!! GTGGTAACCCCAGTTTTTTT+CGG + chr6.1:4699612-4699631 None:intergenic 40.0%
AACAAGACTAATCGGGGTTC+GGG - chr6.1:4700503-4700522 MS.gene91475:CDS 45.0%
AAGACCATCATACGCCCAAT+TGG - chr6.1:4699958-4699977 MS.gene91475:intron 45.0%
AAGACCTAAATGGATGGGAG+AGG - chr6.1:4700418-4700437 MS.gene91475:intron 45.0%
ATCATACGCCCAATTGGTCA+AGG - chr6.1:4699964-4699983 MS.gene91475:intron 45.0%
CAGATGATCATCCTCCTCAA+GGG + chr6.1:4699875-4699894 None:intergenic 45.0%
CCAAGAAGAGTTCATCAGGA+TGG + chr6.1:4700700-4700719 None:intergenic 45.0%
CCATCCTGATGAACTCTTCT+TGG - chr6.1:4700697-4700716 MS.gene91475:CDS 45.0%
GCATCTTTATTAGGGGACTC+TGG + chr6.1:4699931-4699950 None:intergenic 45.0%
GCATTAGCACCATCTTTGCT+AGG + chr6.1:4700087-4700106 None:intergenic 45.0%
GGCTATTAGGCGTCAATTTC+GGG - chr6.1:4700116-4700135 MS.gene91475:intron 45.0%
GGGTGAAAGACCTAAATGGA+TGG - chr6.1:4700412-4700431 MS.gene91475:intron 45.0%
GTAGCCAAGAAGAGTTCATC+AGG + chr6.1:4700704-4700723 None:intergenic 45.0%
GTTGGCTACAACAAGTTTCC+TGG + chr6.1:4699581-4699600 None:intergenic 45.0%
TAAGAGGAAGTCTGGCGATT+GGG - chr6.1:4700727-4700746 MS.gene91475:CDS 45.0%
TCATACGCCCAATTGGTCAA+GGG - chr6.1:4699965-4699984 MS.gene91475:intron 45.0%
TCTTCTTGGCTACTCGTAAG+AGG - chr6.1:4700711-4700730 MS.gene91475:CDS 45.0%
TGTCTATACCACAGGTCGTA+AGG - chr6.1:4700538-4700557 MS.gene91475:CDS 45.0%
TTAGAGAATCAATCAGGCGG+TGG - chr6.1:4701634-4701653 MS.gene91475:intron 45.0%
TTATGGGTAGATGTTGCTGG+GGG - chr6.1:4701361-4701380 MS.gene91475:intron 45.0%
TTCCACCAATGCTCGTTCAA+AGG - chr6.1:4699525-4699544 MS.gene91475:CDS 45.0%
TTGACCAATTGGGCGTATGA+TGG + chr6.1:4699965-4699984 None:intergenic 45.0%
TTTATTAGGGGACTCTGGCT+CGG + chr6.1:4699926-4699945 None:intergenic 45.0%
! AGCTAGATCAGTAGGTTGGA+CGG + chr6.1:4699785-4699804 None:intergenic 45.0%
! ATCTAGTTCGGTAGGTTGGA+CGG + chr6.1:4699722-4699741 None:intergenic 45.0%
! TGGTGCTAATGCAATGAGGA+TGG - chr6.1:4700095-4700114 MS.gene91475:intron 45.0%
! TGTCTTGTGGATTGTTGGTG+AGG + chr6.1:4699650-4699669 None:intergenic 45.0%
! TTGTGGATTGTTGGTGAGGT+GGG + chr6.1:4699646-4699665 None:intergenic 45.0%
!! TACCTTTGAACGAGCATTGG+TGG + chr6.1:4699530-4699549 None:intergenic 45.0%
!! TCACCTCATTTTCAGGAGGA+CGG + chr6.1:4701468-4701487 None:intergenic 45.0%
ACCTTCTCAAGTGACTGCAC+CGG - chr6.1:4699837-4699856 MS.gene91475:intron 50.0%
ACTTGAGAAGGTAGGACCAC+CGG + chr6.1:4699829-4699848 None:intergenic 50.0%
AGACGCGCCGAAAAAAACTG+GGG - chr6.1:4699602-4699621 MS.gene91475:CDS 50.0%
ATCACTACCTTCCCTTGAGG+AGG - chr6.1:4699861-4699880 MS.gene91475:CDS 50.0%
ATGCCGTCCTCCTGAAAATG+AGG - chr6.1:4701462-4701481 MS.gene91475:intron 50.0%
ATGGGAGAGGATGCATATGC+TGG - chr6.1:4700431-4700450 MS.gene91475:intron 50.0%
CTGCAGCAACCAAAGGAACA+AGG + chr6.1:4700247-4700266 None:intergenic 50.0%
GAGACGCGCCGAAAAAAACT+GGG - chr6.1:4699601-4699620 MS.gene91475:CDS 50.0%
GAGGAGGATGATCATCTGCA+AGG - chr6.1:4699877-4699896 MS.gene91475:CDS 50.0%
GATCAGCTTCAGCAGCTCTT+TGG + chr6.1:4701546-4701565 None:intergenic 50.0%
GATCATCTGCAAGGTGTGCA+GGG - chr6.1:4699886-4699905 MS.gene91475:CDS 50.0%
GCAGATGATCATCCTCCTCA+AGG + chr6.1:4699876-4699895 None:intergenic 50.0%
GCATCCTCTCCCATCCATTT+AGG + chr6.1:4700425-4700444 None:intergenic 50.0%
GCTACTCGTAAGAGGAAGTC+TGG - chr6.1:4700719-4700738 MS.gene91475:CDS 50.0%
GGACAGCTAGATCAGTAGGT+TGG + chr6.1:4699789-4699808 None:intergenic 50.0%
GGTTGGACAGCTAGATCAGT+AGG + chr6.1:4699793-4699812 None:intergenic 50.0%
GTAAGAGGAAGTCTGGCGAT+TGG - chr6.1:4700726-4700745 MS.gene91475:CDS 50.0%
GTGCAGTCACTTGAGAAGGT+AGG + chr6.1:4699837-4699856 None:intergenic 50.0%
TCAAGGGAAGGTAGTGATGC+CGG + chr6.1:4699859-4699878 None:intergenic 50.0%
TGATCATCCTCCTCAAGGGA+AGG + chr6.1:4699871-4699890 None:intergenic 50.0%
TGATCATCTGCAAGGTGTGC+AGG - chr6.1:4699885-4699904 MS.gene91475:CDS 50.0%
! ACTGTGGTGCCTTGTTCCTT+TGG - chr6.1:4700235-4700254 MS.gene91475:intron 50.0%
! AGCATGTCAGTAAGGCGCTT+TGG + chr6.1:4700374-4700393 None:intergenic 50.0%
! CTTGTGGATTGTTGGTGAGG+TGG + chr6.1:4699647-4699666 None:intergenic 50.0%
! GCATGTCAGTAAGGCGCTTT+GGG + chr6.1:4700373-4700392 None:intergenic 50.0%
! GGAAGAATTCAGGAAGCGCA+TGG - chr6.1:4701606-4701625 MS.gene91475:intron 50.0%
! GGACATCTAGTTCGGTAGGT+TGG + chr6.1:4699726-4699745 None:intergenic 50.0%
! GGACCACAGTTGATGTCTTG+TGG + chr6.1:4699663-4699682 None:intergenic 50.0%
! GGCAGGTTGGACATCTAGTT+CGG + chr6.1:4699734-4699753 None:intergenic 50.0%
! GGTTGGACATCTAGTTCGGT+AGG + chr6.1:4699730-4699749 None:intergenic 50.0%
!! TCGGGATGTTTTTGCGTGCT+GGG - chr6.1:4700134-4700153 MS.gene91475:intron 50.0%
!! TTCGGGATGTTTTTGCGTGC+TGG - chr6.1:4700133-4700152 MS.gene91475:intron 50.0%
!!! TTTTTTTCGGCGCGTCTCGT+TGG + chr6.1:4699599-4699618 None:intergenic 50.0%
ACTAATCGGGGTTCGGGCAA+AGG - chr6.1:4700509-4700528 MS.gene91475:CDS 55.0%
ATGGTGAGCCTTACGACCTG+TGG + chr6.1:4700549-4700568 None:intergenic 55.0%
CCATGACGTGGCACTAGACA+TGG - chr6.1:4700565-4700584 MS.gene91475:intron 55.0%
CCATGTCTAGTGCCACGTCA+TGG + chr6.1:4700568-4700587 None:intergenic 55.0%
CGAGACGCGCCGAAAAAAAC+TGG - chr6.1:4699600-4699619 MS.gene91475:CDS 55.0%
GGAGGAGCTGTCTATACCAC+AGG - chr6.1:4700530-4700549 MS.gene91475:CDS 55.0%
GGCATCACTACCTTCCCTTG+AGG - chr6.1:4699858-4699877 MS.gene91475:CDS 55.0%
GGTGATATGCAAGAGGTGGG+CGG - chr6.1:4701322-4701341 MS.gene91475:intron 55.0%
GTTGCTGGGGGTTTGTCTTG+AGG - chr6.1:4701373-4701392 MS.gene91475:intron 55.0%
TCGTAAGGCTCACCATGACG+TGG - chr6.1:4700553-4700572 MS.gene91475:intron 55.0%
! AGATCAGTAGGTTGGACGGC+TGG + chr6.1:4699781-4699800 None:intergenic 55.0%
!! GATTGGGTTGACAGTCGTGC+TGG - chr6.1:4700743-4700762 MS.gene91475:CDS 55.0%
AATCGGGGTTCGGGCAAAGG+AGG - chr6.1:4700512-4700531 MS.gene91475:CDS 60.0%
AGGGAGGTTGGATGGCTGGT+CGG + chr6.1:4699756-4699775 None:intergenic 60.0%
GCCGGTGCAGTCACTTGAGA+AGG + chr6.1:4699841-4699860 None:intergenic 60.0%
! AGTAGGTTGGACGGCTGGTC+AGG + chr6.1:4699776-4699795 None:intergenic 60.0%
! AGTTCGGTAGGTTGGACGGC+TGG + chr6.1:4699718-4699737 None:intergenic 60.0%
! GTAGGTTGGACGGCTGGTCA+GGG + chr6.1:4699775-4699794 None:intergenic 60.0%
! GTTGGTGAGGTGGGCTCAAC+TGG + chr6.1:4699637-4699656 None:intergenic 60.0%
ACGTGCTGAGGCTGCTGCAG+CGG - chr6.1:4701519-4701538 MS.gene91475:intron 65.0%
CCAACCTACCCCACCAGCAC+CGG - chr6.1:4699807-4699826 MS.gene91475:intron 65.0%
GAAGGTAGGACCACCGGTGC+TGG + chr6.1:4699823-4699842 None:intergenic 65.0%
GGTCAGGGAGGTTGGATGGC+TGG + chr6.1:4699760-4699779 None:intergenic 65.0%
! CGGTAGGTTGGACGGCTGGT+CGG + chr6.1:4699714-4699733 None:intergenic 65.0%
! GGCTGGTCAGGGAGGTTGGA+TGG + chr6.1:4699764-4699783 None:intergenic 65.0%
! GGGAGGTTGGATGGCTGGTC+GGG + chr6.1:4699755-4699774 None:intergenic 65.0%
! GGTAGGTTGGACGGCTGGTC+GGG + chr6.1:4699713-4699732 None:intergenic 65.0%
! GTAGGTTGGACGGCTGGTCG+GGG + chr6.1:4699712-4699731 None:intergenic 65.0%
!! GTAGGACCACCGGTGCTGGT+GGG + chr6.1:4699819-4699838 None:intergenic 65.0%
!! TAGGACCACCGGTGCTGGTG+GGG + chr6.1:4699818-4699837 None:intergenic 65.0%
GGCTGCAGCAGCACGTGCTG+AGG - chr6.1:4701507-4701526 MS.gene91475:intron 70.0%
GGGGAAGTGGGACGGCTGGT+CGG + chr6.1:4699693-4699712 None:intergenic 70.0%
! ACCTACCCCACCAGCACCGG+TGG - chr6.1:4699810-4699829 MS.gene91475:intron 70.0%
! AGTGGGACGGCTGGTCGGGA+AGG + chr6.1:4699688-4699707 None:intergenic 70.0%
! GAGGTGGGCTCAACTGGCCG+TGG + chr6.1:4699631-4699650 None:intergenic 70.0%
! GGACGGCTGGTCAGGGAGGT+TGG + chr6.1:4699768-4699787 None:intergenic 70.0%
! GGACGGCTGGTCGGGAAGGT+TGG + chr6.1:4699684-4699703 None:intergenic 70.0%
! GGACGGCTGGTCGGGGAAGT+GGG + chr6.1:4699705-4699724 None:intergenic 70.0%
! GGATGGCTGGTCGGGCAGGT+TGG + chr6.1:4699747-4699766 None:intergenic 70.0%
! GGCTGGTCGGGGAAGTGGGA+CGG + chr6.1:4699701-4699720 None:intergenic 70.0%
! GGGAAGTGGGACGGCTGGTC+GGG + chr6.1:4699692-4699711 None:intergenic 70.0%
! GGTAGGACCACCGGTGCTGG+TGG + chr6.1:4699820-4699839 None:intergenic 70.0%
! GGTTGGACGGCTGGTCAGGG+AGG + chr6.1:4699772-4699791 None:intergenic 70.0%
! GGTTGGATGGCTGGTCGGGC+AGG + chr6.1:4699751-4699770 None:intergenic 70.0%
! TGGACGGCTGGTCGGGGAAG+TGG + chr6.1:4699706-4699725 None:intergenic 70.0%
!! ACCACCGGTGCTGGTGGGGT+AGG + chr6.1:4699814-4699833 None:intergenic 70.0%
!! CCGGTGCTGGTGGGGTAGGT+TGG + chr6.1:4699810-4699829 None:intergenic 70.0%
GGTCGGGGAAGTGGGACGGC+TGG + chr6.1:4699697-4699716 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 4699487 4701744 4699487 ID=MS.gene91475
chr6.1 mRNA 4699487 4701744 4699487 ID=MS.gene91475.t1;Parent=MS.gene91475
chr6.1 exon 4701685 4701744 4701685 ID=MS.gene91475.t1.exon1;Parent=MS.gene91475.t1
chr6.1 CDS 4701685 4701744 4701685 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
chr6.1 exon 4701245 4701324 4701245 ID=MS.gene91475.t1.exon2;Parent=MS.gene91475.t1
chr6.1 CDS 4701245 4701324 4701245 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
chr6.1 exon 4701037 4701154 4701037 ID=MS.gene91475.t1.exon3;Parent=MS.gene91475.t1
chr6.1 CDS 4701037 4701154 4701037 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
chr6.1 exon 4700645 4700923 4700645 ID=MS.gene91475.t1.exon4;Parent=MS.gene91475.t1
chr6.1 CDS 4700645 4700923 4700645 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
chr6.1 exon 4700456 4700557 4700456 ID=MS.gene91475.t1.exon5;Parent=MS.gene91475.t1
chr6.1 CDS 4700456 4700557 4700456 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
chr6.1 exon 4699849 4699963 4699849 ID=MS.gene91475.t1.exon6;Parent=MS.gene91475.t1
chr6.1 CDS 4699849 4699963 4699849 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
chr6.1 exon 4699487 4699791 4699487 ID=MS.gene91475.t1.exon7;Parent=MS.gene91475.t1
chr6.1 CDS 4699487 4699791 4699487 ID=cds.MS.gene91475.t1;Parent=MS.gene91475.t1
Gene Sequence

>MS.gene91475

ATGACTAGACAATGTCATGGAACTAAGCGAAAAGAGTCTTCCACCAATGCTCGTTCAAAGGTATTACAACCACGATATCTTGCTAAAGCAACCAGGAAACTTGTTGTAGCCAACGAGACGCGCCGAAAAAAACTGGGGTTACCACGGCCAGTTGAGCCCACCTCACCAACAATCCACAAGACATCAACTGTGGTCCAACCTTCCCGACCAGCCGTCCCACTTCCCCGACCAGCCGTCCAACCTACCGAACTAGATGTCCAACCTGCCCGACCAGCCATCCAACCTCCCTGACCAGCCGTCCAACCTACTGATCTAGCTGTCCAACCTACCCCACCAGCACCGGTGGTCCTACCTTCTCAAGTGACTGCACCGGCATCACTACCTTCCCTTGAGGAGGATGATCATCTGCAAGGTGTGCAGGGAGAAAACACACATACCGAGCCAGAGTCCCCTAATAAAGATGCTATTATAAAGACCATCATACGCCCAATTGGTCAAGGGTATTTTATTTTTGTTTCTTTTACAACTTATCATGTATTAATTTTTATAAAAATATATTTATTTTACAATTACTTTTTTTTTTGTTGTAGATTTACTCCTAGCAAAGATGGTGCTAATGCAATGAGGATGGCTATTAGGCGTCAATTTCGGGATGTTTTTGCGTGCTGGGAAGATGTTCCTCAAGATCTATGGTTTGAAATATTTTCGGTAAGGATATAAAAGAATAGTGTAGCTTCATTGGTTGAAAACTGTGGTGCCTTGTTCCTTTGGTTGCTGCAGAAATATGATACAAACTTTGCTATTTTTATATTGTCTGGCAGGAATATGTTTCATGGGAGACTCAATATGATACGGAAATCAGGAGCATATTTGATAAAAAGTGCCCAAAGCGCCTTACTGACATGCTTACAAAAGTAAGAAATAAGGGTGAAAGACCTAAATGGATGGGAGAGGATGCATATGCTGGTCTTCTTGAATATTGGAAGAGTGACAAATTCAACAAGCTTTCTTCACAAAACAAGACTAATCGGGGTTCGGGCAAAGGAGGAGCTGTCTATACCACAGGTCGTAAGGCTCACCATGACGTGGCACTAGACATGGTATGCATCCATTCCAATTTCTTTAATATCTAGTTCATTGCTAGAATTTGACAGACATTACTATTTGTTTTATGGCTTGACTGACAGTGTAAAATACTTGGGCGACCTATCCATCCTGATGAACTCTTCTTGGCTACTCGTAAGAGGAAGTCTGGCGATTGGGTTGACAGTCGTGCTGGAAAAACTTATGTAAGTTTTTATCGTATAATAATAGAATTGATAATCACTCAAGAAGCTCAAAAAACTTATGTCAGCAATCTGAGATATGTACTTTATATCGAATATGTCTGCAATATTGAGATATGTGAACATATATAATCTGGAATCTTGTACTGAACAACCATAATTCATTATGTGCTTGTGCAGAATTTTTAGTAAGTAGCAGAATTTTTAAAAAAATCAATAACTTACGTCTAATGATCAAAATACATATATGGATAAGTTATGTAAACCTGGAATATTTGCAATTTGAGATATGTATTTTATACCGAATATGTCCACAATCTTGAGATATGTGTTATAAGATATGTGAACACATATAATTTGGAATCTTGTACTGAATAGCCATAATTCATTATGTGCTTCTGCAGAATTTTAGTAAGTAGCTGAATTTTTTAAAATATCGATAACTTATGTCTAATGATCAAACTACATATAGTAATAAGTTGTGTTTTTTTACAGGATAACTATCAGCAGCGCCTGACTCAACTAACAAAGATTGATGAGAATTCTAATGGTGATATGCAAGAGGTGGGCGGAGAGAAACGACTTCAATTATGGGTAGATGTTGCTGGGGGTTTGTCTTGAGGTAATTGTTATGGTACTGGGGCTTTATCTTTCAACATTAGACCAGGTGTAAGTCATCTAACTCAAGAATGCCGTCCTCCTGAAAATGAGGTGATTGAAATGTTTCGTCAGGAGGCTGCAGCAGCACGTGCTGAGGCTGCTGCAGCGGACCAAAGAGCTGCTGAAGCTGATCAGCGTTCACAACAGCTAGAGAATCAATTCTCTACTGCTATGGAAGAATTCAGGAAGCGCATGGAAGCTTTAGAGAATCAATCAGGCGGTGGTTCTTGTAGTACTATTCGATCTCGTCACACTCAACATCCACATTATGATCCTGCTCTTGATGATCAATCAGAGGATGACCTCACCTAA
Protein sequence

>MS.gene91475.t1

MTRQCHGTKRKESSTNARSKGENTHTEPESPNKDAIIKTIIRPIGQGFTPSKDGANAMRMAIRRQFRDVFACWEDVPQDLWFEIFSEYVSWETQYDTEIRSIFDKKCPKRLTDMLTKVRNKGERPKWMGEDAYAGLLEYWKSDKFNKLSSQNKTNRGSGKGGAVYTTGRKAHHDVALDMCKILGRPIHPDELFLATRKRKSGDWVDSRAGKTYDNYQQRLTQLTKIDENSNGDMQEVGGEKRLQLWVDVAGGVSHLTQECRPPENEVIEMFRQEAAAARAEAAAADQRAAEADQRSQQLENQFSTAMEEFRKRMEALENQSGGGSCSTIRSRHTQHPHYDPALDDQSEDDLT