AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92169

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92169.t1 MTR_0036s0070 90.504 337 29 1 255 591 93 426 0.0 628
MS.gene92169.t1 MTR_0036s0050 73.837 344 84 1 255 592 93 436 0.0 525
MS.gene92169.t1 MTR_0036s0110 61.808 343 120 3 260 592 102 443 2.79e-142 420
MS.gene92169.t1 MTR_1g060750 57.396 338 120 5 260 592 59 377 3.66e-122 366
MS.gene92169.t1 MTR_8g096990 45.507 345 172 7 258 591 92 431 1.16e-95 300
MS.gene92169.t1 MTR_5g080890 38.876 427 213 12 191 591 29 433 2.29e-86 275
MS.gene92169.t1 MTR_5g080960 38.642 427 214 12 191 591 29 433 6.35e-86 274
MS.gene92169.t1 MTR_5g044770 42.151 344 184 7 258 591 92 430 5.38e-85 271
MS.gene92169.t1 MTR_8g038280 40.401 349 188 8 258 591 92 435 5.98e-80 259
MS.gene92169.t1 MTR_8g038250 41.880 351 160 9 258 591 92 415 1.32e-78 254
MS.gene92169.t1 MTR_6g090290 39.143 350 192 7 258 591 92 436 1.10e-74 245
MS.gene92169.t1 MTR_3g097150 41.834 349 180 9 258 591 108 448 4.82e-73 241
MS.gene92169.t1 MTR_8g038310 38.462 351 185 8 250 591 85 413 9.72e-70 231
MS.gene92169.t1 MTR_2g059490 39.943 353 191 9 255 590 61 409 1.36e-68 229
MS.gene92169.t1 MTR_4g109760 40.974 349 184 10 256 591 59 398 3.18e-68 227
MS.gene92169.t1 MTR_3g028120 38.617 347 197 7 258 591 95 438 3.74e-68 228
MS.gene92169.t1 MTR_5g008110 36.828 372 216 9 234 591 49 415 7.25e-68 226
MS.gene92169.t1 MTR_2g059460 36.785 367 206 10 242 591 49 406 5.75e-66 221
MS.gene92169.t1 MTR_5g008150 36.364 374 216 10 234 591 45 412 2.60e-65 219
MS.gene92169.t1 MTR_2g067830 35.530 349 211 7 255 591 60 406 7.96e-65 218
MS.gene92169.t1 MTR_0024s0040 37.464 347 199 7 258 591 69 410 7.16e-64 216
MS.gene92169.t1 MTR_2g105300 36.095 338 201 8 263 588 47 381 7.93e-64 214
MS.gene92169.t1 MTR_5g008070 39.655 348 192 10 255 590 84 425 8.41e-64 216
MS.gene92169.t1 MTR_5g038360 33.652 419 238 11 188 591 43 436 3.47e-62 212
MS.gene92169.t1 MTR_6g088760 39.384 292 158 8 260 536 96 383 6.25e-57 200
MS.gene92169.t1 MTR_5g044570 35.632 348 181 10 258 592 95 412 1.19e-55 194
MS.gene92169.t1 MTR_7g117430 34.383 381 217 13 226 589 120 484 5.50e-52 186
MS.gene92169.t1 MTR_4g045857 48.077 208 95 6 258 457 64 266 2.24e-47 168
MS.gene92169.t1 MTR_5g008090 34.783 299 177 8 307 591 20 314 6.62e-45 162
MS.gene92169.t1 MTR_3g046300 34.444 360 200 12 260 589 105 458 1.92e-42 159
MS.gene92169.t1 MTR_1g115210 33.523 352 203 13 255 589 149 486 2.11e-42 159
MS.gene92169.t1 MTR_5g091130 33.333 363 203 14 260 590 199 554 7.83e-42 159
MS.gene92169.t1 MTR_1g039270 31.250 352 212 14 255 589 131 469 2.79e-41 155
MS.gene92169.t1 MTR_1g111350 32.277 347 200 12 260 589 139 467 1.93e-38 148
MS.gene92169.t1 MTR_4g007740 33.724 341 200 12 268 590 146 478 2.57e-37 145
MS.gene92169.t1 MTR_6g085010 30.499 341 213 10 260 589 164 491 9.44e-37 143
MS.gene92169.t1 MTR_2g028190 31.624 351 220 11 255 589 105 451 6.58e-34 134
MS.gene92169.t1 MTR_3g053710 31.356 354 207 13 260 589 125 466 2.29e-29 122
MS.gene92169.t1 MTR_2g055840 26.536 358 230 8 260 589 21 373 2.23e-27 114
MS.gene92169.t1 MTR_5g016260 30.108 372 214 18 246 591 82 433 3.07e-27 116
MS.gene92169.t1 MTR_2g084550 30.788 419 235 23 210 590 37 438 3.22e-27 115
MS.gene92169.t1 MTR_5g016260 30.108 372 214 18 246 591 82 433 4.99e-27 116
MS.gene92169.t1 MTR_4g056420 29.781 366 217 21 256 590 81 437 6.33e-27 114
MS.gene92169.t1 MTR_7g105850 31.043 393 199 20 254 591 92 467 1.86e-26 113
MS.gene92169.t1 MTR_3g053980 32.184 348 206 14 260 589 150 485 6.83e-26 111
MS.gene92169.t1 MTR_7g102750 30.831 373 204 20 256 590 85 441 2.52e-24 107
MS.gene92169.t1 MTR_3g068000 29.017 417 217 20 225 589 52 441 4.18e-24 106
MS.gene92169.t1 MTR_8g103880 27.300 337 219 10 255 577 119 443 1.01e-23 104
MS.gene92169.t1 MTR_1g061940 29.178 377 215 20 254 592 85 447 8.76e-23 102
MS.gene92169.t1 MTR_3g014290 29.512 410 237 19 203 590 38 417 5.76e-22 99.0
MS.gene92169.t1 MTR_4g095270 28.090 356 214 16 260 591 88 425 7.24e-22 100
MS.gene92169.t1 MTR_1g037630 29.268 410 238 19 203 590 50 429 9.73e-22 98.6
MS.gene92169.t1 MTR_4g095270 28.090 356 214 16 260 591 88 425 1.11e-21 99.8
MS.gene92169.t1 MTR_7g080230 27.322 366 230 12 256 592 75 433 1.59e-21 98.2
MS.gene92169.t1 MTR_7g102750 31.250 352 188 20 256 569 85 420 2.57e-21 97.4
MS.gene92169.t1 MTR_3g065160 27.493 371 206 15 260 591 82 428 3.10e-21 97.1
MS.gene92169.t1 MTR_2g070430 29.396 381 214 20 254 591 89 457 5.43e-21 96.7
MS.gene92169.t1 MTR_4g100990 28.093 388 202 18 271 590 93 471 2.38e-20 94.7
MS.gene92169.t1 MTR_8g039540 27.317 410 249 17 202 590 53 434 3.95e-20 93.6
MS.gene92169.t1 MTR_7g021510 26.613 372 227 17 256 592 76 436 4.82e-20 94.0
MS.gene92169.t1 MTR_1g078240 27.419 372 223 16 256 592 73 432 3.06e-19 91.3
MS.gene92169.t1 MTR_1g078240 27.346 373 223 16 256 592 73 433 2.66e-18 88.6
MS.gene92169.t1 MTR_1g078240 31.679 262 147 11 256 494 73 325 2.59e-17 84.7
MS.gene92169.t1 MTR_5g012490 26.250 400 210 19 262 590 85 470 2.96e-17 85.1
MS.gene92169.t1 MTR_4g008110 39.823 113 42 3 329 438 48 137 3.50e-17 79.0
MS.gene92169.t1 MTR_7g062360 26.133 375 228 16 254 589 61 425 4.83e-17 84.3
MS.gene92169.t1 MTR_5g061690 26.488 336 218 12 260 577 109 433 6.82e-17 84.3
MS.gene92169.t1 MTR_8g075010 25.556 360 220 14 260 589 108 449 2.31e-16 82.8
MS.gene92169.t1 MTR_3g087780 25.833 360 221 16 260 589 107 450 8.00e-16 80.9
MS.gene92169.t1 MTR_7g080230 26.686 341 218 12 276 592 31 363 8.05e-16 80.1
MS.gene92169.t1 MTR_5g077850 27.838 370 210 14 260 591 89 439 1.74e-15 79.3
MS.gene92169.t1 MTR_1g029810 26.542 373 219 18 254 591 59 411 2.13e-15 79.3
MS.gene92169.t1 MTR_1g029810 26.542 373 219 18 254 591 59 411 2.17e-15 79.0
MS.gene92169.t1 MTR_6g084710 26.791 321 207 14 260 567 199 504 7.31e-15 78.2
MS.gene92169.t1 MTR_6g084710 28.235 255 167 10 260 505 199 446 1.43e-14 77.0
MS.gene92169.t1 MTR_0488s0020 30.357 224 131 7 2 223 279 479 3.99e-14 75.5
MS.gene92169.t1 MTR_7g006745 34.804 204 109 8 46 243 224 409 4.82e-14 74.7
MS.gene92169.t1 MTR_3g096930 22.928 362 232 15 260 591 92 436 4.13e-13 72.0
MS.gene92169.t1 MTR_0945s0010 31.707 123 81 3 471 590 1 123 2.21e-12 65.9
MS.gene92169.t1 MTR_3g100500 28.090 356 217 13 255 589 100 437 5.76e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92169.t1 AT5G33340 45.349 344 176 4 258 591 95 436 1.91e-85 273
MS.gene92169.t1 AT1G31450 44.413 358 173 9 258 592 90 444 1.24e-81 263
MS.gene92169.t1 AT1G64830 44.058 345 177 7 258 591 91 430 2.36e-81 262
MS.gene92169.t1 AT2G35615 43.575 358 174 10 260 592 92 446 2.65e-80 260
MS.gene92169.t1 AT2G28010 39.316 351 175 11 255 591 67 393 1.13e-67 225
MS.gene92169.t1 AT2G03200 39.216 357 186 10 258 591 112 460 2.08e-65 221
MS.gene92169.t1 AT2G28030 38.462 351 178 11 255 591 63 389 1.31e-62 212
MS.gene92169.t1 AT2G28040 37.280 397 211 13 209 590 9 382 1.38e-62 211
MS.gene92169.t1 AT2G28040 37.726 387 203 13 219 590 28 391 1.58e-61 209
MS.gene92169.t1 AT2G28220 37.960 353 181 14 255 591 69 399 1.50e-56 196
MS.gene92169.t1 AT2G28225 37.079 356 180 12 255 591 69 399 1.83e-56 196
MS.gene92169.t1 AT1G01300 32.706 425 242 16 196 590 75 485 3.57e-46 170
MS.gene92169.t1 AT3G61820 34.817 382 213 13 232 589 114 483 1.52e-43 162
MS.gene92169.t1 AT2G23945 32.487 394 202 19 235 589 80 448 5.37e-40 152
MS.gene92169.t1 AT4G30030 33.616 354 196 16 258 589 83 419 5.85e-39 148
MS.gene92169.t1 AT3G18490 31.214 346 208 13 260 589 169 500 6.41e-39 150
MS.gene92169.t1 AT3G25700 29.508 366 217 12 260 590 91 450 2.99e-38 147
MS.gene92169.t1 AT2G42980 31.649 376 195 12 260 590 167 525 5.28e-38 147
MS.gene92169.t1 AT3G20015 29.821 446 268 17 166 589 48 470 6.90e-38 146
MS.gene92169.t1 AT3G59080 31.967 366 205 15 260 590 177 533 1.52e-37 146
MS.gene92169.t1 AT1G25510 32.849 344 202 13 260 589 155 483 4.53e-36 141
MS.gene92169.t1 AT5G10770 32.759 348 215 10 255 590 134 474 1.44e-35 140
MS.gene92169.t1 AT4G30040 31.148 366 203 14 249 584 81 427 1.34e-32 130
MS.gene92169.t1 AT1G79720 33.523 352 185 15 269 589 149 482 1.40e-32 131
MS.gene92169.t1 AT5G36260 29.155 367 219 14 256 592 81 436 1.34e-27 117
MS.gene92169.t1 AT3G12700 28.329 353 227 11 260 590 113 461 5.30e-27 114
MS.gene92169.t1 AT5G10760 33.724 341 200 13 260 589 139 464 6.77e-27 114
MS.gene92169.t1 AT3G50050 29.972 357 207 17 260 591 100 438 2.05e-26 114
MS.gene92169.t1 AT3G59080 30.303 363 179 15 260 590 177 497 1.00e-25 111
MS.gene92169.t1 AT3G02740 28.297 364 221 16 256 590 88 440 1.27e-24 107
MS.gene92169.t1 AT3G54400 30.645 310 186 14 255 552 90 382 1.73e-24 106
MS.gene92169.t1 AT5G45120 27.660 423 217 16 241 589 71 478 1.80e-24 107
MS.gene92169.t1 AT5G43100 27.195 353 218 15 260 590 83 418 2.28e-24 108
MS.gene92169.t1 AT3G02740 28.297 364 221 16 256 590 13 365 2.95e-24 105
MS.gene92169.t1 AT5G43100 27.684 354 215 16 260 590 83 418 3.64e-24 106
MS.gene92169.t1 AT1G08210 31.148 366 210 18 256 590 121 475 6.17e-24 106
MS.gene92169.t1 AT1G08210 31.148 366 210 18 256 590 135 489 7.44e-24 106
MS.gene92169.t1 AT1G08210 31.148 366 210 18 256 590 87 441 7.59e-24 105
MS.gene92169.t1 AT5G22850 30.831 373 209 23 256 592 84 443 8.00e-24 105
MS.gene92169.t1 AT1G66180 28.378 370 207 16 260 591 79 428 8.14e-24 104
MS.gene92169.t1 AT3G52500 30.318 409 182 22 254 590 91 468 1.17e-22 101
MS.gene92169.t1 AT2G39710 29.067 375 209 18 254 585 66 426 6.84e-22 99.4
MS.gene92169.t1 AT2G36670 29.199 387 231 19 238 592 19 394 1.33e-21 98.2
MS.gene92169.t1 AT2G36670 29.199 387 231 19 238 592 19 394 1.33e-21 98.2
MS.gene92169.t1 AT4G12920 26.632 383 246 14 221 589 26 387 1.89e-21 97.1
MS.gene92169.t1 AT2G36670 28.667 450 261 25 179 592 40 465 2.06e-21 98.2
MS.gene92169.t1 AT2G36670 29.396 381 226 19 244 592 91 460 2.78e-21 97.8
MS.gene92169.t1 AT1G09750 26.611 357 226 13 255 589 106 448 4.12e-21 97.1
MS.gene92169.t1 AT5G24820 27.520 367 219 14 255 589 48 399 7.96e-21 95.5
MS.gene92169.t1 AT5G37540 28.726 369 207 16 260 589 87 438 1.83e-20 94.7
MS.gene92169.t1 AT1G08210 31.085 341 193 18 256 565 87 416 5.64e-20 94.0
MS.gene92169.t1 AT5G02190 28.075 374 210 19 262 589 82 442 6.13e-20 93.2
MS.gene92169.t1 AT1G05840 26.829 369 228 15 256 592 83 441 1.27e-18 89.7
MS.gene92169.t1 AT1G65240 25.824 364 232 15 256 592 77 429 1.91e-17 85.9
MS.gene92169.t1 AT4G16563 28.223 287 138 12 364 590 214 492 1.53e-15 80.1
MS.gene92169.t1 AT1G49050 26.582 237 153 11 271 494 223 451 5.50e-15 78.2
MS.gene92169.t1 AT1G77480 24.444 360 232 16 260 591 74 421 1.05e-14 77.4
MS.gene92169.t1 AT1G77480 24.444 360 232 16 260 591 74 421 1.12e-14 77.0
MS.gene92169.t1 AT5G07030 28.732 355 221 16 214 552 74 412 2.35e-13 72.8
MS.gene92169.t1 AT5G10080 24.176 364 231 14 260 592 107 456 2.41e-13 73.2
MS.gene92169.t1 AT3G42550 24.789 355 194 12 260 590 85 390 3.66e-13 72.4
MS.gene92169.t1 AT1G44130 27.953 254 146 11 254 491 50 282 1.04e-12 70.9
MS.gene92169.t1 AT4G33490 23.836 365 223 13 260 589 67 411 1.48e-11 67.0
MS.gene92169.t1 AT3G51340 21.972 355 239 12 258 589 108 447 2.61e-11 66.6
MS.gene92169.t1 AT3G25700 35.833 120 66 5 260 369 91 209 7.29e-11 64.7

Find 103 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAATGAAACACAAAATT+AGG 0.174509 2.4:-27594056 None:intergenic
TTGCTATTTGAGTTTAAATT+TGG 0.187752 2.4:-27593723 None:intergenic
ATGAAAGGTATTTCCATCTT+TGG 0.201179 2.4:+27594158 MS.gene92169:CDS
CAACCAAGTTCTGAGGTTTA+AGG 0.223072 2.4:-27594094 None:intergenic
CTCTCTTTGTTGGCTCAATT+TGG 0.230572 2.4:-27587493 None:intergenic
TCAAATAGCAAAATGAAATT+TGG 0.248015 2.4:+27593735 MS.gene92169:CDS
CCACATCCATGGTAGCCTTT+GGG 0.253599 2.4:-27593438 None:intergenic
GGAGATGAAGCAATTATAAA+AGG 0.259280 2.4:+27593756 MS.gene92169:CDS
GGTGATCACTCTTACACTTT+TGG 0.267271 2.4:+27593495 MS.gene92169:CDS
GAAACTGATTTACCTCATAA+AGG 0.269219 2.4:+27587623 MS.gene92169:CDS
GGTAGGGTATTGTTTCTGTT+TGG 0.269988 2.4:-27587032 None:intergenic
GTAATTGGAAACTTAGTTCT+TGG 0.275900 2.4:-27594258 None:intergenic
CAACTAGACCCGTAGCTTTA+CGG 0.294559 2.4:-27593620 None:intergenic
GCTTCCTTGAAGGGAGTTAT+TGG 0.296078 2.4:+27593963 MS.gene92169:CDS
GCTGTTGTTGCTCTCTTTGT+TGG 0.298724 2.4:-27587503 None:intergenic
CCCTACCAAAACAACCAATT+TGG 0.302189 2.4:+27587048 MS.gene92169:CDS
GTAGGGTATTGTTTCTGTTT+GGG 0.311490 2.4:-27587031 None:intergenic
TTTGAATTATCAACAATGAT+AGG 0.325171 2.4:-27587116 None:intergenic
GGTAACATAATCATTGATTC+AGG 0.326198 2.4:+27593894 MS.gene92169:CDS
TTGTGTGTCTGCATCCTTCA+TGG 0.329386 2.4:-27587297 None:intergenic
TCCACATCCATGGTAGCCTT+TGG 0.338920 2.4:-27593439 None:intergenic
AAACTGATTTACCTCATAAA+GGG 0.343480 2.4:+27587624 MS.gene92169:CDS
CAAAACATCTGTGTCAAAAC+AGG 0.344370 2.4:+27587076 MS.gene92169:CDS
GATCGGATTCATGGCACTTC+AGG 0.345422 2.4:-27587256 None:intergenic
CGATGCCAAATTGGTTGTTT+TGG 0.360377 2.4:-27587053 None:intergenic
AACCAAGTTCTGAGGTTTAA+GGG 0.361825 2.4:-27594093 None:intergenic
AATTCTTGCAGACATCAATA+TGG 0.368871 2.4:-27586974 None:intergenic
TCCTTCATGGCCTGAGTGAT+AGG 0.381007 2.4:-27587284 None:intergenic
AGCTGAGCAGAGACTTCTTC+GGG 0.390363 2.4:+27587595 MS.gene92169:CDS
GGGAACATTGTACGCAAATA+AGG 0.390878 2.4:-27593314 None:intergenic
ATTTGGAGGAATCAAACAAT+AGG 0.396296 2.4:-27593706 None:intergenic
GGTACTGAATCCATTAACTT+TGG 0.398522 2.4:+27593525 MS.gene92169:CDS
CAGAACTTGGTTGTTGATCA+TGG 0.399287 2.4:+27594104 MS.gene92169:CDS
TTGTGCTCTATTTCCAAAGA+TGG 0.401939 2.4:-27594171 None:intergenic
TGTGAGTATATATACACTTA+TGG 0.402832 2.4:+27593474 MS.gene92169:CDS
CATCATCAAAATTTGTGTCT+TGG 0.403398 2.4:-27587451 None:intergenic
TAAAATGAAACACAAAATTA+GGG 0.403931 2.4:-27594055 None:intergenic
TACCCTTAAACCTCAGAACT+TGG 0.428511 2.4:+27594091 MS.gene92169:CDS
TGAAATCCATCACTGCTTGA+AGG 0.434273 2.4:-27587653 None:intergenic
AAGATGGAAATACCTTTCAT+TGG 0.438491 2.4:-27594155 None:intergenic
GATTCGAAGATTTGATTATG+AGG 0.444366 2.4:-27593800 None:intergenic
TAGGGTCGTGATCGGATTCA+TGG 0.452460 2.4:-27587265 None:intergenic
AACACCAATAACTCCCTTCA+AGG 0.454045 2.4:-27593967 None:intergenic
CTATTTGAGTTTAAATTTGG+AGG 0.456554 2.4:-27593720 None:intergenic
TGATTTGGTAGCTTCCTTGA+AGG 0.464165 2.4:+27593953 MS.gene92169:CDS
CACATCCATGGTAGCCTTTG+GGG 0.466661 2.4:-27593437 None:intergenic
AAGCTGAGCAGAGACTTCTT+CGG 0.470848 2.4:+27587594 MS.gene92169:CDS
ATTCGAAGATTTGATTATGA+GGG 0.473153 2.4:-27593799 None:intergenic
GCTACGGGTCTAGTTGGTCT+TGG 0.478890 2.4:+27593627 MS.gene92169:CDS
GAAGGCGCAACCCCTTTATG+AGG 0.484323 2.4:-27587635 None:intergenic
GAACTTTGTTATATCTCCTA+AGG 0.488128 2.4:-27594033 None:intergenic
ACATCAAAAGCGTTACTTTG+CGG 0.495014 2.4:-27587233 None:intergenic
ATCAATGATTATGTTACCAT+CGG 0.500077 2.4:-27593889 None:intergenic
TTTGAAGACAAATGCGGCTA+AGG 0.500449 2.4:+27586995 MS.gene92169:CDS
TCACAACTAGGTGATTCAAT+TGG 0.503436 2.4:+27593672 MS.gene92169:CDS
ACATGACAAACCCATGAAAG+TGG 0.505680 2.4:-27593391 None:intergenic
TAAAAGAAGCCGTAAAGCTA+CGG 0.507090 2.4:+27593611 MS.gene92169:CDS
GCAACAGCAAGCCTTTCAAC+AGG 0.507502 2.4:-27593276 None:intergenic
GATTTGGTAGCTTCCTTGAA+GGG 0.510053 2.4:+27593954 MS.gene92169:CDS
CGTAAAGCTACGGGTCTAGT+TGG 0.511569 2.4:+27593621 MS.gene92169:CDS
GGTCTAGTTGGTCTTGGAGC+AGG 0.513423 2.4:+27593633 MS.gene92169:CDS
CCCTATCACTCAGGCCATGA+AGG 0.514564 2.4:+27587283 MS.gene92169:CDS
GAAGCAATTATAAAAGGTAA+TGG 0.516167 2.4:+27593762 MS.gene92169:CDS
TGTAACCACTGCATGAAAGT+TGG 0.518619 2.4:+27586948 None:intergenic
TGGTGATGCCTTCGAGATTG+AGG 0.520017 2.4:-27593836 None:intergenic
AAGCAGTGATGGATTTCAGT+TGG 0.532790 2.4:+27587658 MS.gene92169:CDS
CCCAAAGGCTACCATGGATG+TGG 0.533147 2.4:+27593438 MS.gene92169:CDS
CTATCACTAGTTTCACAACT+AGG 0.533914 2.4:+27593660 MS.gene92169:CDS
AGGCTTGCTGTTGCAGACAC+AGG 0.537207 2.4:+27593285 MS.gene92169:CDS
TAGTTCTTGGAACAATCAGT+TGG 0.541167 2.4:-27594245 None:intergenic
AAAAGAAGCCGTAAAGCTAC+GGG 0.541737 2.4:+27593612 MS.gene92169:CDS
GAAGGGAGTTATTGGTGTTG+AGG 0.543742 2.4:+27593971 MS.gene92169:CDS
GGCAAACATAACAGATAATG+TGG 0.545587 2.4:+27587571 MS.gene92169:CDS
CCTTCATGGCCTGAGTGATA+GGG 0.548338 2.4:-27587283 None:intergenic
GGAACAATCAGTTGGAGCAA+AGG 0.550575 2.4:-27594237 None:intergenic
GTTGCGCCTTCAAGCAGTGA+TGG 0.550580 2.4:+27587647 MS.gene92169:CDS
TTACTCCCCAAAGGCTACCA+TGG 0.551474 2.4:+27593432 MS.gene92169:CDS
AAGACAAATGCGGCTAAGGA+TGG 0.554324 2.4:+27586999 MS.gene92169:CDS
AGTTGTGAAACTAGTGATAG+TGG 0.555072 2.4:-27593657 None:intergenic
TTCGAAGATTTGATTATGAG+GGG 0.558132 2.4:-27593798 None:intergenic
ACTTCTGTCAAGAGCAGCTA+CGG 0.562514 2.4:+27587716 MS.gene92169:CDS
TCATAATAAGGAGTGTCTTG+TGG 0.564393 2.4:-27593354 None:intergenic
TTGTTTGTCGTACCAATGAA+AGG 0.565953 2.4:+27594143 MS.gene92169:CDS
CTGAGTGATAGGGTCGTGAT+CGG 0.566594 2.4:-27587273 None:intergenic
GAAACTGAAGCTAGCTCGCA+AGG 0.575850 2.4:+27587368 MS.gene92169:CDS
GGCTTGCTGTTGCAGACACA+GGG 0.577382 2.4:+27593286 MS.gene92169:CDS
TTAGTAGGGTACGATCTCAA+AGG 0.581764 2.4:+27594203 MS.gene92169:CDS
CAGCAAGCCTTTCAACAGGT+GGG 0.584660 2.4:-27593272 None:intergenic
AACTGATTTACCTCATAAAG+GGG 0.589373 2.4:+27587625 MS.gene92169:CDS
TAGGCACCCCACCTGTTGAA+AGG 0.592550 2.4:+27593265 MS.gene92169:intron
ACAGCAAGCCTTTCAACAGG+TGG 0.594361 2.4:-27593273 None:intergenic
AGCAAGCCTTTCAACAGGTG+GGG 0.603962 2.4:-27593271 None:intergenic
GTAACATAATCATTGATTCA+GGG 0.608837 2.4:+27593895 MS.gene92169:CDS
AATCTCGAAGGCATCACCAT+CGG 0.614952 2.4:+27593840 MS.gene92169:CDS
TGATCAACAACCAAGTTCTG+AGG 0.624623 2.4:-27594101 None:intergenic
AAAAGATATTGTTAATAACG+TGG 0.634286 2.4:+27587136 MS.gene92169:CDS
AAAACATCTGTGTCAAAACA+GGG 0.638365 2.4:+27587077 MS.gene92169:CDS
CGATCACGACCCTATCACTC+AGG 0.654575 2.4:+27587274 MS.gene92169:CDS
TCTTGCTCATTACTCCCCAA+AGG 0.655591 2.4:+27593423 MS.gene92169:CDS
ACTTCTAAGAATTAGTTGTG+TGG 0.667067 2.4:-27587408 None:intergenic
CAAGAATTTGAAGACAAATG+CGG 0.669403 2.4:+27586989 MS.gene92169:CDS
TGTGTCAAAACAGGGCAATG+TGG 0.672808 2.4:+27587085 MS.gene92169:CDS
GTTCAGACAGGTCAAACCGA+TGG 0.685598 2.4:+27593873 MS.gene92169:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.4 gene 27586960 27594268 27586960 ID=MS.gene92169
chr2.4 mRNA 27586960 27594268 27586960 ID=MS.gene92169.t1;Parent=MS.gene92169
chr2.4 exon 27586960 27587737 27586960 ID=MS.gene92169.t1.exon1;Parent=MS.gene92169.t1
chr2.4 CDS 27586960 27587737 27586960 ID=cds.MS.gene92169.t1;Parent=MS.gene92169.t1
chr2.4 exon 27593268 27594268 27593268 ID=MS.gene92169.t1.exon2;Parent=MS.gene92169.t1
chr2.4 CDS 27593268 27594268 27593268 ID=cds.MS.gene92169.t1;Parent=MS.gene92169.t1
Gene Sequence

>MS.gene92169

ATGAAAGTTGGTCACCATATTGATGTCTGCAAGAATTTGAAGACAAATGCGGCTAAGGATGGTGAAGAAATCCCAAACAGAAACAATACCCTACCAAAACAACCAATTTGGCATCGCAAAACATCTGTGTCAAAACAGGGCAATGTGGTCACTGATCCTATCATTGTTGATAATTCAAAAGATATTGTTAATAACGTGGAAAATACGAATTCTCGCAAATTTTTGGCAAAAAATCATGCAGATTCAAGTGGAGCCAAAAAGAATTTGGAGATACCGCAAAGTAACGCTTTTGATGTCCTGAAGTGCCATGAATCCGATCACGACCCTATCACTCAGGCCATGAAGGATGCAGACACACAACTTGAAAGAGAAGTCAACTTGCAGCTAGATAATCAAGCAGAATGTTGTGAAACTGAAGCTAGCTCGCAAGGAACAGAATTTGTTGATGCCACACAACTAATTCTTAGAAGTGATGACAATGATAGTTTGAACCAAGACACAAATTTTGATGATGATAGTGATGATGCAAATGACCAAATTGAGCCAACAAAGAGAGCAACAACAGCTGAAGAGATAGAGCAGCATAATAGAGATTTTCTCAAGCAGTCATGGGCAAACATAACAGATAATGTGGAAGCTGAGCAGAGACTTCTTCGGGATCTTGAAACTGATTTACCTCATAAAGGGGTTGCGCCTTCAAGCAGTGATGGATTTCAGTTGGTTGTGAGATCAAAAGATAAAGCCGTAAAGAAGAAAACTTCTGTCAAGAGCAGCTACGGTACTAGAGCTAAGGCTGGTAACCCCAAACCATTTAGATGAAGATTCTATACTGGAACATAAGGGGTTTAGCTAACACCCCTTCAAGGTTAGCTCTAAAAAGACTTCTCTTATTACACAAACCTGATATGGTTTTTGTTGCTGAACCATGGATGAATTATATCAATTTCCCTCCCTCTTGGTTTCGGAGATTAGGGTTTAAGATTTTCTCCTTCAATGAAAGAGGAAATCTTTTGCCTAATCTTTGGTGTATATGCTCCTTACAACTTACCCCAACTATCATTGATATAGATAACCAGCAAGTTACCTTCAATCTTTTAATAAATAACAGCCTTGTTTTCTTTTCTGCAATTTACGCATCCACCAGTAACCTTAATAGACAAAACCTTTGGCATAAACTCAACACCCTCCAAGCGACCCATAATGCTCCCTGGACTTTTATAGGAGACTTTAATGCTATTATGGGAGCCCATGAACATTCTGGTAGCTTCTCTCCAGCCAGACCCCCCATCAATGACTTTATATCCTTCACTGACTCTTTCAACCTTATTCACTTACCAACAAGAGGGACCTTTTTCACTTGGAACAACCGTAGATCAGGTAGGAGACACACCAAAAGAAGATTAGATAGAACAATTGTGAACCAACAAATGATTGACCTTTGCTCCTCTATCTCTTGCTCCACTTTAACAAATACTAGTTCAGATCACTTTCCAATTCTTCTAGAATTCAAAACTGATGCCATCAAATTTGCCTCCTCATTCAAGTTCCTTAAAATGTGGACGTTGCACAATGATTGTAAGCAACTCATTTCTGATTGTTGGAATGTTAATGTTGTTGGCTGCCCAATGTTTGTACTTACTACCAAGCTGAAAAATCTCAAAGTTAAGCTCAAACATTGGAACAAAGACGTGTTTGGGAATATTCATGACTATGTGGATGCTGCTGAAACAAAGCTCGATGATATTCAAAATACCATAAACCTACAAGGCCACTCAGATGAACTCATGAATGAAGAAAAGCAAGCACAAACGGACCTTGACCAAGCCCTTAATAAACAGGAAACCTTCTGGAAGGAGAAAGCTAGAGTGAACTGGCATGTGAATGGTGATAGAAACACTAGTTTTTTCCATAGAGTCACAAAAATTAAGAACAAAACAAAAGTTATTTCCTCTATCAGAAATGAAGAGGAAATTATTACGGATCCTCAGAGAATTTCAAATCATGTGGTTAATTATTATAAAAACTTGTTTTCTTCTAACTTTGTTTTGCAGGACTCTTTGCTAGTGGAAGAAGTCATTCCTACTATGATTGATGATCAAACAAATAGACTGCTGACAATGATACCTTCTGCTGATGAGATTAAAGCTGCTGTGTTTGGTTTGAATAGTGATGGAGCTCCGGGGCCAGATGGCTTTGGTGCTTGTTTCTATCACACTTATTGGGAGATAGTAAAGGAAGATGTTTCTAATGCTGTTCTTCAATTCTTCAATACCGGTTGGTTGCTTCCTCACTTCAATGCGAATTCTTTAGTCCTCATCCCAAAAAACCCTAATGCTGATACTATTGATCAATACAGACCTATAGCCTTAGCAAATTTTAAATTCAAAATTATCTCTAAGGTTATTGCTGATAGGCTTGCAAATATTCTCCCCACTATTATCTCTGAGGAACAAAGAGGATTCATAAAGGGCAGGAACATAAAAGACTGTATTGCTTTAGCCTCGGAAGCAATTAATGTCTTAGACAAAAGAAGCTTTGGTGGAAATCTTGCATTGAAAATTGATGTTTCCAAGGCATTTGATACAGTTAATTGGGAGTTCCTTCTTAAAGTATTGACATGTTTTGGTTTTAATCATACATTCTGCAATTGGATAAGGGCAATTCTTCATTCAGCTAATGTCTCTATTTGTATCAATGGTAGTCAAGAAGGATATTTCAATTGCAAAAGAGGTGTTAGACAGGGGGATCCCCTCTCCCCTCTTCTTTTTTGTATTGCCGAAGAAGTTCTTAGTAGAGGAATAAAGAAGTTAGTGTCTGAGAACAAGCTTGAGTTAATTTCTGCATCTAGAACTGCTAAGCTTCCATCCCATTGTTTCTATGCTGATGATTTAATGGTTTATTGCAAAGGTAAAATGGCAGGTCTCCAAGCCTTAAAGGAGCTGTTCACAAGATATGCCATGTGCTCTGGTCAAATCATGAATCTTAGGAAGTCTTCAATCCATGCTGGAGGAATCAGCCATCAAAGGTTAACCAATATTGCGGTTTTCTTAGGATTTTCTGTAGGATCCTTACCTTTCACTTACCTAGGAGCACCGATCTTTAAAGGGAAGCCAAAGAGAAGACACTTTCAATCCATTGCGGATAAGGTGAAGCTCAAATTTGCATCTTGGAAAGCTTCTCTCCTTTCTATTGCAGGCAGAATCCAGCTAGTAAAATCTGTGGTACAATCAATGCTCCTCCACACTATGGCAATTTACTCTTGGCCAGTCAGTTTACTCAGAGATATAGAAAAATGGATAAAAAACTTTATTTGGAGCGGAGACATCACTAAGAGGAAACTTGTTACGGTGGCGTGGAAAAAAATTTGCGCATCTTATGATGAAGGAGGGCTTAACATCAGATCACTTGTCTGCTTAAATGAAGCCACTAATCTTAAACTATGCTGGGATTTATTACATTCACAGGAACAATGGGCATTCATTCTCAAGAGTAGGGTTTTAAGAGATGTTTCTTGTATCAATTATCACATTTATTCTTCCATATGGAGCAGTATCAAAATGGAATTTCAAACTGTTAGAGAAAACTCTACTTGGTTGGTTGGTGATGGGAATCAAATTAATTTTTGGTTCGATTCTTGGTGTGGAGACCCATTGCAACACTCTCTCAATGTTAGTACTGATCTTATGCATGATTATCCTCAAAAACTTAGTGCTTATATTGCCAATTCTTCTTGGAATTTACCAACCACCTTGGCTCAAGCTCTCCCGGATTTGAGAAGAATTGTAAATCAAGTTACCCTTCCAGTGAATGATCAACGTGACTGCTTGGTTTGGAAGCATAACTCATCAGGTAATTTAACTCTTAAAGATGCGTTTGAATTCAAAAAGCACCACTTTCCAAAACTTGCTTGGACAAAATCAATCTGGTCAATGGACATACCTCCCTCTAAATCTTTTTTGGCTTGGAGGCTCATGCATAACAAGCTTCCCACAGATGATAACTTAGCTACAAGAGGTTGTTTACTTCCTTCTATGTGCTCTTTGTGCAAGACAAATTATGAAACTTCATTTCATCTTTTTTTCCAATGTTCTTATGCTTGCAATATCTGGAAATGGTTTGCATCCATCTTAAATATCAACTTTCAGCTTCATAATATGGAGGACATCTGGAAGATTTGTCAAAGGAACTGGAATCCTCAATGCAAGCTCACAATCACTGCTGCTCTAATCAATATCCTCAATGCTATTTGGTATTCCAGAAACCAACAAAGATTCAAAGACAAAAAAATTCATTGGAGGTCTGCTATTTCCACAATCATTTCAAATGTCTCCCTATCTAGTAATCTTTCCAACACAGTAGCTTCATCCTCAATATCTGATTTTATAATTTTGAAGAAGTTTAATGTTTGTATTCACCCCCCAAAGGCTCCTAAAATTATTGAAGTTGTCTGGCATCCTCCAATCTTCCGTTGGATCAAATGCAACACGGATGGGTCAGCAACCAACACGTCCTCATCCTGTGGTGGAATATTTAGGGACAAAGACGCTAAGTTCTTGCTCTGCTTTGCTGAAAACACCGGTATAGGTAGTGCATACCATGCTGAACTCTCAGGGGCTATGAGAGCAATTGAGATTGCAGTGCAGCATCAGTGGACCTCTCTCTGGTTGGAATCAGATTCAGCCCTTGTTGTAAATGCTTTTAAAAATCACTCTCTCGTTCCTTGGAGACTCCGAAATCGCTGGACTAATTGCATGCTTAACATCAGCTCCATGAACTTCTTGGTAACTCATATATATAGAGAAGGTAATAGTTGTGCTGATGCACTAGCTAATTTTGGTCTTTCCCTTGATACTTTAACTATTTGGCTGGAAATCCCAATGTGCATTAGAAGTTTTTATTTTCAAAACAGGCATGGCTTGCCTTGTTTTAGGTTTGTAAACTTCTAAAGAGGTTTGGTTTGGCCCCCCTCTTTTTCATTGTTCTTTTCTTGTTTCTCTTTTTTTTTGGTAATTTATTTTGGATAGTCTTAGCTCCTTGCTAAGCTATCTTTGCATAAAAAAAAAAAAAAGGAGGATTAGGGCAAACATGATAGCCAAGCAAGTCTAGCTTTTATAATAAAGGATTTTACAAAATTATAGTTTAATAATTTATAAAAAAGATATATTAATAGAATTGAAGGAAGGGAGAGTAATGAATACTAAAATGTATGATAGGAAAAGAAACACAAATGTTTGATTGATATTGTAAAACGATCTATATTTAAAACAATTTTTTACATAATGATTTATAATTTGGGATAGACGAAGTTCTATATTTGATAAAATTAGAGGCAAATAACTCACATTTGAAAAGTCTCATCAAGCATGCATGTTTGATAAAAGTATAAATATTGGGGAGAAGTGGAATGAATGGTTTGTGGTCATTAAAGTGCTCCTATTTATAGATTTCATTTAGTACTAATTGATGAGACTTTTCAAATGTAAGTTATTTCCCTCTAATACCGGAATTCTTGTTTTCTGCCCTATGTTCACTTTATTCCCCATCCTTTGTTGATATGTTCACTTTATTCCCCATCCTTTGTTGAATCTATTAGAAATCGTGGTTGGGCCTAACACAACCCCACAAAACCGGAATTCTTGTTTTCTGCCCTATGTTCACTTTATTCCCCATCCTTTGTTGATATGTTCACTTTATTCCCCATCCTTTGTTGAATCTATTAGAAATCGTGGTTGGGCCTAACACAACCCCACAAAACCGGATTGTGAGGTGAGGATTACCCCCACTTATAAACAAATTGTCAGGCCAACTCCTAACCGATGTGGGACTCTTAACACACCCCCTCACGCTCAGGATTAGACATCTGAAGAGTGAACATAAATGACGGGTGGCCCGATAACAGAATGGCCCGATAACAGAAACCTGACAGCAGGTGGCCCAGAATCGTGAAGAGGCTCTGATACCATGTTAGAAATGGTGGTTGGGCCTAACCCCACAAAACTGGCTTCTGAGGTGAGGATTGCCCCCACTTATAAACAAATTGTCAGGCCAACTCCTAACCGATGTGGGACTCTTAACAGAATCCACTAAAAACCCTAGTGGCTTCGAAGTCGAACTTATTCATCATGATTCACCATTGTCACCCTTTTACAACTCTTCCCTTACCTCATCGGAGTTACTCACAAATGCGGCCTTTCGCCCTATTTCACGTTCCAAATGACTTTCCCTTTCTCAAAATAACGAGTTAAACAAATCGCCAGAATCTATTATAATTCCAAATGGTGGTGATTACCTCATGAAAATCTATATAGGCACCCCACCTGTTGAAAGGCTTGCTGTTGCAGACACAGGGAGTGACCTTATTTGCGTACAATGTTCCCCTTGTGAAAATTGTTTTCCACAAGACACTCCTTATTATGATCCAAAAAAATCTTCCACTTTCATGGGTTTGTCATGTGACTCACAATCTTGCTCATTACTCCCCAAAGGCTACCATGGATGTGGAAAATCAAATCAGTGTGAGTATATATACACTTATGGTGATCACTCTTACACTTTTGGAGATTTGGGTACTGAATCCATTAACTTTGGAGAAAAAGATGTTACATTTCCTAAATCAATTTTTGGATGTGGGCATCAAAATGATGTCACATTTAAAAGAAGCCGTAAAGCTACGGGTCTAGTTGGTCTTGGAGCAGGACCACTATCACTAGTTTCACAACTAGGTGATTCAATTGGTCACAAATTTTCCTATTGTTTGATTCCTCCAAATTTAAACTCAAATAGCAAAATGAAATTTGGAGATGAAGCAATTATAAAAGGTAATGGCGTTGTGTCTACTCCCCTCATAATCAAATCTTCGAATCCTTCTTTTTACTTCCTCAATCTCGAAGGCATCACCATCGGACAAAAAACTGTTCAGACAGGTCAAACCGATGGTAACATAATCATTGATTCAGGGACAACATTGACGTATCTTGAACAAAACTTTTACAATGATTTGGTAGCTTCCTTGAAGGGAGTTATTGGTGTTGAGGAAGTGAATGATCCTCCATCACCATATACTTTTTGCTTCACCTTAGGAGATATAACAAAGTTCCCTAATTTTGTGTTTCATTTTACGGGAGCTGATGTTACCCTTAAACCTCAGAACTTGGTTGTTGATCATGGCAACAACTCGTATTGCTTGTTTGTCGTACCAATGAAAGGTATTTCCATCTTTGGAAATAGAGCACAATTTAATTTTTTAGTAGGGTACGATCTCAAAGGAAAAACAGTTTCCTTTGCTCCAACTGATTGTTCCAAGAACTAA
Protein sequence

>MS.gene92169.t1

MKVGHHIDVCKNLKTNAAKDGEEIPNRNNTLPKQPIWHRKTSVSKQGNVVTDPIIVDNSKDIVNNVENTNSRKFLAKNHADSSGAKKNLEIPQSNAFDVLKCHESDHDPITQAMKDADTQLEREVNLQLDNQAECCETEASSQGTEFVDATQLILRSDDNDSLNQDTNFDDDSDDANDQIEPTKRATTAEEIEQHNRDFLKQSWANITDNVEAEQRLLRDLETDLPHKGVAPSSSDGFQLVVRSKDKAVKKKTSVKSSYGTPPVERLAVADTGSDLICVQCSPCENCFPQDTPYYDPKKSSTFMGLSCDSQSCSLLPKGYHGCGKSNQCEYIYTYGDHSYTFGDLGTESINFGEKDVTFPKSIFGCGHQNDVTFKRSRKATGLVGLGAGPLSLVSQLGDSIGHKFSYCLIPPNLNSNSKMKFGDEAIIKGNGVVSTPLIIKSSNPSFYFLNLEGITIGQKTVQTGQTDGNIIIDSGTTLTYLEQNFYNDLVASLKGVIGVEEVNDPPSPYTFCFTLGDITKFPNFVFHFTGADVTLKPQNLVVDHGNNSYCLFVVPMKGISIFGNRAQFNFLVGYDLKGKTVSFAPTDCSKN