AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92301.t1 MTR_5g006180 95.105 572 14 1 1 572 1 558 0.0 1113
MS.gene92301.t1 MTR_5g090110 77.954 567 74 5 8 572 7 524 0.0 881
MS.gene92301.t1 MTR_4g115780 61.008 595 203 8 1 571 101 690 0.0 662
MS.gene92301.t1 MTR_4g107920 57.041 561 206 13 19 569 10 545 0.0 574
MS.gene92301.t1 MTR_7g024390 37.228 505 290 10 29 523 9 496 7.38e-99 314
MS.gene92301.t1 MTR_7g024580 37.426 505 289 10 29 523 9 496 1.43e-98 313
MS.gene92301.t1 MTR_4g130540 36.853 502 296 10 29 523 8 495 2.32e-98 313
MS.gene92301.t1 MTR_4g063720 36.634 505 293 10 29 523 9 496 7.80e-97 308
MS.gene92301.t1 MTR_7g099680 36.170 517 279 11 29 523 9 496 1.62e-96 308
MS.gene92301.t1 MTR_4g063710 36.832 505 292 10 29 523 9 496 1.62e-96 308
MS.gene92301.t1 MTR_4g103780 40.570 456 151 7 118 569 1 340 2.20e-94 292
MS.gene92301.t1 MTR_2g038260 35.317 521 293 14 29 530 9 504 2.83e-87 283
MS.gene92301.t1 MTR_8g032650 35.271 516 302 14 28 531 8 503 5.49e-87 282
MS.gene92301.t1 MTR_7g025770 34.308 513 308 12 29 530 9 503 1.62e-86 281
MS.gene92301.t1 MTR_2g437440 35.603 514 301 12 29 530 9 504 2.68e-86 280
MS.gene92301.t1 MTR_7g451380 36.078 510 306 10 29 531 9 505 9.52e-86 279
MS.gene92301.t1 MTR_2g032550 35.700 507 296 13 29 523 10 498 3.17e-85 277
MS.gene92301.t1 MTR_7g026170 35.882 510 304 13 29 530 9 503 4.17e-85 277
MS.gene92301.t1 MTR_7g025620 33.704 540 328 13 29 557 9 529 4.86e-85 277
MS.gene92301.t1 MTR_8g099945 33.969 524 297 12 19 523 29 522 8.24e-85 278
MS.gene92301.t1 MTR_8g099795 33.206 524 307 10 16 523 26 522 4.64e-84 275
MS.gene92301.t1 MTR_7g026090 33.456 544 324 14 29 554 9 532 5.65e-84 274
MS.gene92301.t1 MTR_7g026110 34.100 522 317 13 28 539 8 512 3.61e-83 272
MS.gene92301.t1 MTR_7g025760 34.653 505 305 11 28 523 8 496 1.09e-82 271
MS.gene92301.t1 MTR_2g017730 32.749 513 308 11 29 523 8 501 2.87e-82 269
MS.gene92301.t1 MTR_7g029415 35.340 515 299 17 29 530 16 509 8.24e-82 268
MS.gene92301.t1 MTR_7g074460 33.463 514 305 12 28 535 8 490 1.32e-81 267
MS.gene92301.t1 MTR_7g021440 35.714 504 299 11 29 523 9 496 3.16e-81 267
MS.gene92301.t1 MTR_5g066800 34.942 518 295 15 29 523 9 507 6.80e-80 263
MS.gene92301.t1 MTR_7g025820 33.594 512 315 11 29 531 9 504 8.57e-80 263
MS.gene92301.t1 MTR_1g017130 33.595 509 277 15 29 530 9 463 2.67e-79 261
MS.gene92301.t1 MTR_2g022050 36.699 515 296 13 28 530 8 504 4.11e-79 261
MS.gene92301.t1 MTR_7g026130 35.271 516 298 17 29 529 9 503 6.98e-79 263
MS.gene92301.t1 MTR_7g026030 33.078 523 318 14 29 539 9 511 1.21e-78 260
MS.gene92301.t1 MTR_7g025720 33.465 508 306 11 28 523 8 495 7.62e-78 258
MS.gene92301.t1 MTR_7g025790 33.074 514 314 15 29 531 9 503 5.65e-77 255
MS.gene92301.t1 MTR_2g038270 32.752 516 321 13 30 531 10 513 6.48e-77 256
MS.gene92301.t1 MTR_7g026140 35.088 513 288 16 29 530 9 487 8.25e-77 255
MS.gene92301.t1 MTR_4g079590 33.004 506 310 13 29 523 9 496 1.40e-75 252
MS.gene92301.t1 MTR_7g025630 34.357 521 302 14 28 539 8 497 1.44e-75 251
MS.gene92301.t1 MTR_6g033760 32.495 517 301 14 27 523 7 495 1.57e-75 252
MS.gene92301.t1 MTR_6g033850 31.507 511 310 11 27 521 7 493 1.88e-74 250
MS.gene92301.t1 MTR_7g025260 36.034 469 259 12 79 523 17 468 3.25e-74 248
MS.gene92301.t1 MTR_2g032580 33.140 516 301 13 28 532 8 490 5.11e-74 247
MS.gene92301.t1 MTR_7g078210 34.884 516 303 17 29 531 9 504 1.18e-73 247
MS.gene92301.t1 MTR_7g024030 32.879 514 315 14 29 531 9 503 1.86e-73 246
MS.gene92301.t1 MTR_4g027145 33.333 519 317 14 29 530 9 515 2.05e-73 246
MS.gene92301.t1 MTR_7g011590 33.137 510 305 12 29 530 9 490 1.69e-72 243
MS.gene92301.t1 MTR_2g010020 31.915 517 289 11 30 523 58 534 5.57e-72 244
MS.gene92301.t1 MTR_2g010020 31.915 517 289 11 30 523 57 533 5.87e-72 243
MS.gene92301.t1 MTR_8g103930 32.402 537 285 16 27 530 9 500 1.17e-71 241
MS.gene92301.t1 MTR_4g092920 29.982 547 311 12 5 523 29 531 4.64e-71 241
MS.gene92301.t1 MTR_7g078190 33.786 515 311 16 29 531 9 505 4.78e-71 240
MS.gene92301.t1 MTR_6g033955 31.881 505 321 12 27 521 7 498 6.69e-71 240
MS.gene92301.t1 MTR_6g033820 32.874 508 312 14 27 521 7 498 7.23e-70 238
MS.gene92301.t1 MTR_7g025740 32.233 515 312 12 28 523 8 504 2.26e-69 236
MS.gene92301.t1 MTR_6g033870 32.375 522 297 16 27 521 8 500 9.04e-68 232
MS.gene92301.t1 MTR_7g451360 33.268 511 260 13 29 531 9 446 1.27e-66 226
MS.gene92301.t1 MTR_4g092920 33.079 393 232 7 141 523 26 397 6.48e-63 216
MS.gene92301.t1 MTR_2g005690 31.765 510 307 16 27 523 69 550 1.36e-61 216
MS.gene92301.t1 MTR_7g007000 32.233 515 273 18 29 530 9 460 1.86e-61 213
MS.gene92301.t1 MTR_6g033800 31.553 412 269 7 112 521 2 402 5.10e-60 209
MS.gene92301.t1 MTR_2g040160 33.753 397 233 12 29 414 1109 1486 1.12e-52 194
MS.gene92301.t1 MTR_2g032160 32.895 380 237 9 28 400 8 376 1.00e-51 184
MS.gene92301.t1 MTR_2g040370 32.836 402 224 10 29 411 1029 1403 1.82e-51 191
MS.gene92301.t1 MTR_2g040370 33.248 391 214 11 29 399 1413 1776 4.96e-50 187
MS.gene92301.t1 MTR_2g040370 32.836 402 224 10 29 411 1029 1403 2.08e-51 191
MS.gene92301.t1 MTR_2g040370 33.498 406 223 11 29 414 1413 1791 3.99e-50 187
MS.gene92301.t1 MTR_2g040220 32.398 392 239 11 30 410 1019 1395 5.76e-51 189
MS.gene92301.t1 MTR_2g040220 59.184 49 20 0 475 523 1399 1447 1.15e-11 68.6
MS.gene92301.t1 MTR_7g035155 30.350 514 273 18 29 530 9 449 8.99e-50 181
MS.gene92301.t1 MTR_4g063720 43.689 206 109 2 321 523 224 425 1.45e-48 178
MS.gene92301.t1 MTR_4g063720 32.287 223 141 4 29 250 9 222 5.37e-25 109
MS.gene92301.t1 MTR_2g040260 31.807 393 246 9 29 414 1038 1415 1.02e-47 180
MS.gene92301.t1 MTR_2g040350 32.242 397 241 11 30 414 1039 1419 1.50e-46 176
MS.gene92301.t1 MTR_2g040350 32.242 397 241 11 30 414 821 1201 1.55e-46 176
MS.gene92301.t1 MTR_1g041565 28.974 497 267 15 29 521 5 419 2.16e-46 171
MS.gene92301.t1 MTR_4g115860 67.480 123 40 0 395 517 1 123 3.08e-45 156
MS.gene92301.t1 MTR_6g034005 29.384 422 273 10 104 521 4 404 6.88e-45 169
MS.gene92301.t1 MTR_4g115870 41.600 250 77 7 92 338 57 240 7.51e-45 159
MS.gene92301.t1 MTR_7g026080 33.865 251 148 8 29 273 9 247 2.00e-35 134
MS.gene92301.t1 MTR_1g077230 31.597 288 174 9 29 307 9 282 2.52e-30 120
MS.gene92301.t1 MTR_7g026050 30.769 260 140 8 270 523 1 226 1.77e-28 117
MS.gene92301.t1 MTR_1g077240 36.216 185 113 3 348 531 3 183 1.08e-27 113
MS.gene92301.t1 MTR_4g012050 25.105 474 316 11 30 476 4 465 6.56e-27 116
MS.gene92301.t1 MTR_4g115785 65.789 76 26 0 467 542 7 82 6.69e-27 104
MS.gene92301.t1 MTR_2g031670 33.469 245 142 9 41 275 43 276 9.93e-27 111
MS.gene92301.t1 MTR_6g033855 25.521 384 191 9 108 488 8 299 5.32e-26 108
MS.gene92301.t1 MTR_3g051300 25.571 438 292 13 30 455 4 419 1.03e-25 112
MS.gene92301.t1 MTR_4g088515 30.255 314 178 10 29 333 276 557 1.74e-25 111
MS.gene92301.t1 MTR_4g088515 38.235 102 59 2 363 463 521 619 1.74e-11 67.4
MS.gene92301.t1 MTR_5g005870 32.995 197 124 3 329 523 75 265 5.97e-25 107
MS.gene92301.t1 MTR_2g102180 23.385 449 313 10 30 461 4 438 9.66e-23 103
MS.gene92301.t1 MTR_5g092570 24.494 445 302 13 30 459 4 429 8.06e-21 97.1
MS.gene92301.t1 MTR_6g033810 27.193 228 157 5 221 440 63 289 5.51e-19 88.2
MS.gene92301.t1 MTR_3g081170 22.561 492 323 15 23 479 20 488 1.25e-17 87.4
MS.gene92301.t1 MTR_7g074540 46.667 90 47 1 446 535 4 92 2.24e-16 78.2
MS.gene92301.t1 MTR_6g478100 30.097 206 115 7 90 293 39 217 2.76e-15 77.4
MS.gene92301.t1 MTR_1207s0010 32.168 143 89 2 134 275 7 142 4.70e-15 73.9
MS.gene92301.t1 MTR_5g097190 30.396 227 115 9 29 240 158 356 6.58e-14 73.9
MS.gene92301.t1 MTR_7g026180 34.959 123 78 2 98 219 23 144 9.79e-13 70.1
MS.gene92301.t1 MTR_7g451390 44.928 69 37 1 455 523 2 69 2.96e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92301.t1 AT2G32120 73.860 570 135 2 5 571 3 561 0.0 897
MS.gene92301.t1 AT2G32120 73.860 570 135 2 5 571 3 561 0.0 897
MS.gene92301.t1 AT3G12580 38.020 505 286 11 29 523 9 496 4.31e-102 322
MS.gene92301.t1 AT5G02500 37.030 505 291 10 29 523 9 496 1.99e-99 315
MS.gene92301.t1 AT3G09440 37.228 505 290 10 29 523 9 496 4.24e-98 312
MS.gene92301.t1 AT3G09440 37.228 505 290 10 29 523 9 496 4.24e-98 312
MS.gene92301.t1 AT3G09440 37.228 505 290 10 29 523 9 496 4.24e-98 312
MS.gene92301.t1 AT3G09440 37.228 505 290 10 29 523 9 496 4.24e-98 312
MS.gene92301.t1 AT1G16030 36.047 516 285 10 27 523 6 495 5.58e-98 311
MS.gene92301.t1 AT1G56410 37.030 505 291 10 29 523 9 496 8.09e-95 302
MS.gene92301.t1 AT5G02490 35.842 505 297 10 29 523 9 496 2.66e-94 302
MS.gene92301.t1 AT1G09080 34.469 499 306 8 30 523 43 525 5.84e-86 280
MS.gene92301.t1 AT1G09080 34.469 499 306 8 30 523 53 535 6.79e-86 281
MS.gene92301.t1 AT5G42020 33.268 514 320 10 16 523 25 521 2.55e-85 279
MS.gene92301.t1 AT5G42020 33.268 514 320 10 16 523 30 526 4.20e-85 278
MS.gene92301.t1 AT5G28540 33.800 500 309 9 30 523 38 521 3.56e-84 276
MS.gene92301.t1 AT5G42020 33.464 511 316 11 16 519 25 518 3.60e-84 275
MS.gene92301.t1 AT5G09590 30.843 522 305 10 22 523 52 537 1.56e-68 234
MS.gene92301.t1 AT4G37910 31.238 525 299 11 22 523 47 532 1.90e-65 226
MS.gene92301.t1 AT4G37910 31.238 525 299 11 22 523 47 532 1.90e-65 226
MS.gene92301.t1 AT5G02500 34.097 393 239 8 29 414 9 388 4.73e-63 216
MS.gene92301.t1 AT5G49910 31.299 508 312 14 27 523 78 559 6.27e-61 214
MS.gene92301.t1 AT4G24280 31.434 509 310 15 27 523 78 559 1.33e-59 211
MS.gene92301.t1 AT1G11660 26.247 461 306 16 30 476 4 444 5.69e-27 116
MS.gene92301.t1 AT1G11660 26.247 461 306 16 30 476 4 444 5.69e-27 116
MS.gene92301.t1 AT1G11660 26.247 461 306 16 30 476 4 444 5.69e-27 116
MS.gene92301.t1 AT1G79930 24.722 449 307 10 30 461 4 438 1.43e-25 112
MS.gene92301.t1 AT1G79930 24.722 449 307 10 30 461 4 438 1.56e-25 112
MS.gene92301.t1 AT1G11660 27.250 400 259 15 91 476 26 407 6.81e-24 106
MS.gene92301.t1 AT1G79920 24.276 449 309 10 30 461 4 438 1.97e-23 105
MS.gene92301.t1 AT1G79920 24.276 449 309 10 30 461 4 438 2.51e-23 105
MS.gene92301.t1 AT1G79920 24.276 449 309 10 30 461 4 438 2.51e-23 105
MS.gene92301.t1 AT1G79920 24.276 449 309 10 30 461 4 438 2.51e-23 105
MS.gene92301.t1 AT4G16660 24.722 360 238 10 143 480 164 512 1.66e-18 90.1
MS.gene92301.t1 AT4G16660 24.722 360 238 10 143 480 140 488 1.84e-18 90.1

Find 162 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGATATTTCAAGATAA+TGG 0.182846 5.1:+1070620 MS.gene92301:CDS
TGGCACCATAGGTCCTATTT+AGG 0.200225 5.1:-1070821 None:intergenic
AGAAGTCCTTGCAATGTTTC+TGG 0.201625 5.1:+1069585 MS.gene92301:CDS
TCCATGCACACACTGATTTC+AGG 0.246998 5.1:-1070669 None:intergenic
TTCACTAACGCAGCAATAAA+TGG 0.261599 5.1:-1069535 None:intergenic
CAGTGATCCCTTTGGAAACT+TGG 0.262026 5.1:+1070401 MS.gene92301:CDS
TCAATTCCAATCGCTATTTC+AGG 0.266817 5.1:-1069232 None:intergenic
GTGGTGTTCCTTGGAATTAC+AGG 0.282170 5.1:-1070489 None:intergenic
CTGATTTCAGGAACTCCTTT+TGG 0.283375 5.1:-1070657 None:intergenic
TTCAAAGACGAAGCTCCTTC+TGG 0.283594 5.1:+1069355 MS.gene92301:CDS
GAAAGCTTCTCAAGAAACTA+TGG 0.285521 5.1:+1069798 MS.gene92301:CDS
TGTTCAGGTTGATGTAGATT+TGG 0.316072 5.1:+1070092 MS.gene92301:CDS
CTATGGTGCCACAATGGATT+TGG 0.318656 5.1:+1070833 MS.gene92301:CDS
AACATTGCAAGGACTTCTTC+TGG 0.325894 5.1:-1069580 None:intergenic
AAATGTGGTGCTTACTGTTC+CGG 0.327314 5.1:+1069654 MS.gene92301:CDS
TTAGGATATTTCAAGATAAT+GGG 0.332403 5.1:+1070621 MS.gene92301:CDS
ACAGCAGTGGCGTTATTATA+TGG 0.334823 5.1:+1069763 MS.gene92301:CDS
ATAGCGATTGGAATTGATAT+TGG 0.340874 5.1:+1069238 MS.gene92301:CDS
TAACAGGCACCACAGGTATA+CGG 0.347738 5.1:-1070746 None:intergenic
GGCACCATAGGTCCTATTTA+GGG 0.356331 5.1:-1070820 None:intergenic
TACCATCGGTGGAGAAGATT+TGG 0.359082 5.1:+1069933 MS.gene92301:CDS
GTTCAGGTTGATGTAGATTT+GGG 0.369664 5.1:+1070093 MS.gene92301:CDS
GGCATCCTCACTTCCATAAC+AGG 0.369992 5.1:-1070762 None:intergenic
AACGCCACTGCTGTTGGTTC+AGG 0.370548 5.1:-1069754 None:intergenic
ATCAACCTGAACACTGCTTT+GGG 0.377089 5.1:-1070082 None:intergenic
ATGGAAGGAGCTGTTGCTTC+AGG 0.378268 5.1:+1070375 MS.gene92301:CDS
CATCAACCTGAACACTGCTT+TGG 0.383070 5.1:-1070083 None:intergenic
TTCCACCCAAAGCAGTGTTC+AGG 0.388302 5.1:+1070077 MS.gene92301:CDS
GTAAGCACCACATTTCTTAT+CGG 0.392583 5.1:-1069646 None:intergenic
CACGACCATGAGATGCTGTT+TGG 0.393050 5.1:+1069400 MS.gene92301:CDS
TCGCACAAGCACGCTCTAAA+CGG 0.395103 5.1:-1069702 None:intergenic
TATACCTGTGGTGCCTGTTA+TGG 0.395756 5.1:+1070749 MS.gene92301:CDS
CATGTAAACATTGGATGATA+AGG 0.397163 5.1:-1070197 None:intergenic
TCCAAGGTCTGCACCAGAAA+TGG 0.397431 5.1:-1069499 None:intergenic
GCAGAAGAAAACCACTTGTT+AGG 0.403341 5.1:+1070603 MS.gene92301:CDS
TGGCTGGTCTTCATGTTCTC+AGG 0.414478 5.1:+1069725 MS.gene92301:CDS
TTATAAAGGTATAAACCCTT+TGG 0.418915 5.1:+1070329 MS.gene92301:CDS
AAAATAACAGAAGTTTATAA+AGG 0.432441 5.1:+1070315 MS.gene92301:CDS
CATGATGCAAAGGTAGAAGT+TGG 0.434870 5.1:+1070216 MS.gene92301:CDS
CCTTTGGAAGGTGCGGTTTG+TGG 0.435575 5.1:+1070345 MS.gene92301:CDS
CACGCTCTAAACGGGTCAGC+TGG 0.440361 5.1:-1069693 None:intergenic
GGTGTGCTGAGGCCCTAAAT+AGG 0.444919 5.1:+1070808 MS.gene92301:CDS
GCCATTTCTGGTGCAGACCT+TGG 0.447888 5.1:+1069498 MS.gene92301:CDS
ATGATGCAAAGGTAGAAGTT+GGG 0.448859 5.1:+1070217 MS.gene92301:CDS
GCTGGCATTGTGGTGTTCCT+TGG 0.449119 5.1:-1070498 None:intergenic
GCATCTGACAGTGAAACAAC+TGG 0.449771 5.1:+1069187 MS.gene92301:CDS
ATCAATTTATGCCTTCTTGA+GGG 0.450343 5.1:-1070859 None:intergenic
CTGCGTTAGTGAACAATGTC+TGG 0.455549 5.1:+1069545 MS.gene92301:CDS
TCAGGCATCAGTGATCCCTT+TGG 0.456587 5.1:+1070393 MS.gene92301:CDS
TGTTGACAGGGCGGAGTTTG+AGG 0.457797 5.1:+1070140 MS.gene92301:CDS
CTGGTGCAGACCTTGGACAT+TGG 0.457903 5.1:+1069505 MS.gene92301:CDS
GCCATTGGAATTCGAGCTGA+TGG 0.458770 5.1:+1070453 MS.gene92301:CDS
AAAGCTGATGAATTGCATCC+TGG 0.459890 5.1:-1070056 None:intergenic
AAAATATATTCAAGCGTCAT+GGG 0.460640 5.1:+1069992 MS.gene92301:CDS
ATAAATGATGTGATACTTGT+TGG 0.460986 5.1:+1070243 MS.gene92301:CDS
CGCACAAGCACGCTCTAAAC+GGG 0.463935 5.1:-1069701 None:intergenic
GTAACAAGATTCTCCACCCT+TGG 0.466538 5.1:-1070282 None:intergenic
GAGCGTGCTTGTGCGATGGC+TGG 0.467592 5.1:+1069709 MS.gene92301:CDS
TTAGCCGGTGTTGTGATGCC+TGG 0.471501 5.1:+1070720 MS.gene92301:CDS
TGGAAACGACTGAATGAAAC+CGG 0.482736 5.1:-1069673 None:intergenic
CAAGCCACTCCTCTTGCCAT+TGG 0.482744 5.1:+1070438 MS.gene92301:CDS
CCAATCGCTATTTCAGGAAG+AGG 0.484412 5.1:-1069226 None:intergenic
GTTGATGTAGATTTGGGAGA+TGG 0.485617 5.1:+1070099 MS.gene92301:CDS
TATAATAACGCCACTGCTGT+TGG 0.489694 5.1:-1069760 None:intergenic
GGTCAAAGAAATTAAATCAA+TGG 0.491607 5.1:+1070014 MS.gene92301:CDS
TAACAAGATTCTCCACCCTT+GGG 0.492275 5.1:-1070281 None:intergenic
GAAAATATATTCAAGCGTCA+TGG 0.496398 5.1:+1069991 MS.gene92301:CDS
CAAATCCATTGTGGCACCAT+AGG 0.497810 5.1:-1070832 None:intergenic
CCTCTTCCTGAAATAGCGAT+TGG 0.506297 5.1:+1069226 MS.gene92301:CDS
GTTGCTGTGACTGCTACCGC+CGG 0.506470 5.1:+1069877 MS.gene92301:CDS
TTGCTGTGACTGCTACCGCC+GGG 0.511136 5.1:+1069878 MS.gene92301:CDS
AACAAGTCCAAGTTTCCAAA+GGG 0.511681 5.1:-1070408 None:intergenic
AGATATGCAAGGTTGTTGAC+AGG 0.512232 5.1:+1070127 MS.gene92301:CDS
TATCAATTTATGCCTTCTTG+AGG 0.516603 5.1:-1070860 None:intergenic
AACAGGCACCACAGGTATAC+GGG 0.517047 5.1:-1070745 None:intergenic
ATTGCTTCGAGTTGCAGCCC+AGG 0.521502 5.1:+1070038 MS.gene92301:CDS
GGAATACCTGCAGCACCAAA+AGG 0.524506 5.1:+1070642 MS.gene92301:CDS
CAAACAGCATCTCATGGTCG+TGG 0.528773 5.1:-1069399 None:intergenic
CACAAACCGCACCTTCCAAA+GGG 0.529952 5.1:-1070344 None:intergenic
TTCAATATGAAACGCTTGAT+CGG 0.530493 5.1:+1069433 MS.gene92301:CDS
CCTTTGCATCATGTAAACAT+TGG 0.531721 5.1:-1070206 None:intergenic
GAGGCCCTAAATAGGACCTA+TGG 0.533257 5.1:+1070816 MS.gene92301:CDS
TGCGGTTTGTGGTGCAGCAA+TGG 0.533347 5.1:+1070356 MS.gene92301:CDS
ACCACAGGTATACGGGATCC+AGG 0.535468 5.1:-1070738 None:intergenic
TCCTGAAATCAGTGTGTGCA+TGG 0.540172 5.1:+1070668 MS.gene92301:CDS
TCTTGAAATATCCTAACAAG+TGG 0.545629 5.1:-1070614 None:intergenic
AAGCTGATGAATTGCATCCT+GGG 0.547863 5.1:-1070055 None:intergenic
AAAAGTAGATCTTTACGTGC+TGG 0.548488 5.1:-1070516 None:intergenic
AACCTGAACACTGCTTTGGG+TGG 0.551609 5.1:-1070079 None:intergenic
AAGGACTTCTTCTGGAGTGG+TGG 0.559811 5.1:-1069572 None:intergenic
TGCAAGGACTTCTTCTGGAG+TGG 0.559852 5.1:-1069575 None:intergenic
TCCCAGATAAAAGCATTGGC+AGG 0.560848 5.1:+1069907 MS.gene92301:CDS
TCTCAAGAAACTATGGGCAG+TGG 0.563843 5.1:+1069805 MS.gene92301:CDS
GGATTTGGTTACCCTCAAGA+AGG 0.565408 5.1:+1070848 MS.gene92301:CDS
AACCAAATCTTCTCCACCGA+TGG 0.568389 5.1:-1069935 None:intergenic
TGGACGAACGCCAATGTCCA+AGG 0.570950 5.1:-1069515 None:intergenic
GTACGAAGGTGAGGGAAAGA+AGG 0.571023 5.1:+1070581 MS.gene92301:CDS
TTTAGAGCGTGCTTGTGCGA+TGG 0.572607 5.1:+1069705 MS.gene92301:CDS
TCAGATGCAACGGTATATGC+AGG 0.578112 5.1:-1069172 None:intergenic
GATACTTGTGTACGAAGGTG+AGG 0.578994 5.1:+1070572 MS.gene92301:CDS
AAAGGTATAAACCCTTTGGA+AGG 0.581870 5.1:+1070333 MS.gene92301:CDS
TGTTTCACTGTCAGATGCAA+CGG 0.582470 5.1:-1069182 None:intergenic
CCCATCAGCTCGAATTCCAA+TGG 0.583989 5.1:-1070454 None:intergenic
TAACAAGTCCAAGTTTCCAA+AGG 0.584586 5.1:-1070409 None:intergenic
GAGGATGTTCTTATATCCCA+AGG 0.584781 5.1:+1070265 MS.gene92301:CDS
CCATGACCATCATCAACCAT+TGG 0.585720 5.1:-1070783 None:intergenic
AGTCCTTGCAATGTTTCTGG+TGG 0.586924 5.1:+1069588 MS.gene92301:CDS
AGTATCCCAGATAAAAGCAT+TGG 0.589832 5.1:+1069903 MS.gene92301:CDS
CCACAAACCGCACCTTCCAA+AGG 0.591096 5.1:-1070345 None:intergenic
TGATGCAAAGGTAGAAGTTG+GGG 0.591780 5.1:+1070218 MS.gene92301:CDS
ACTTCCATAACAGGCACCAC+AGG 0.592348 5.1:-1070753 None:intergenic
CCAATGTTTACATGATGCAA+AGG 0.592560 5.1:+1070206 MS.gene92301:CDS
TGCTGTGACTGCTACCGCCG+GGG 0.595836 5.1:+1069879 MS.gene92301:CDS
CATCTGACAGTGAAACAACT+GGG 0.598563 5.1:+1069188 MS.gene92301:CDS
ATGGTCGTGGGAGAATTGAC+TGG 0.601519 5.1:-1069386 None:intergenic
CCATTGGAATTCGAGCTGAT+GGG 0.601553 5.1:+1070454 MS.gene92301:CDS
CACAGAAACACTACATTGTG+AGG 0.604708 5.1:-1069263 None:intergenic
AGTTTGTACCTGTAATTCCA+AGG 0.609145 5.1:+1070481 MS.gene92301:CDS
AAAGACGAAGCTCCTTCTGG+AGG 0.611357 5.1:+1069358 MS.gene92301:CDS
ATCTTTACGTGCTGGCATTG+TGG 0.613475 5.1:-1070508 None:intergenic
AATTCCAATGGCAAGAGGAG+TGG 0.613750 5.1:-1070442 None:intergenic
AAACAGCATCTCATGGTCGT+GGG 0.616728 5.1:-1069398 None:intergenic
TATCTCCAAACAGCATCTCA+TGG 0.617418 5.1:-1069405 None:intergenic
GTTTGTGGTGCAGCAATGGA+AGG 0.619096 5.1:+1070360 MS.gene92301:CDS
TGGTCATGGTTGGTGTGCTG+AGG 0.620833 5.1:+1070797 MS.gene92301:CDS
AAAGCTTCTCAAGAAACTAT+GGG 0.624225 5.1:+1069799 MS.gene92301:CDS
AATGATGTGATACTTGTTGG+AGG 0.629718 5.1:+1070246 MS.gene92301:CDS
TAGGACCTATGGTGCCACAA+TGG 0.630018 5.1:+1070827 MS.gene92301:CDS
TGGTGCCTGTTATGGAAGTG+AGG 0.631903 5.1:+1070757 MS.gene92301:CDS
TGACAGGGCGGAGTTTGAGG+AGG 0.633647 5.1:+1070143 MS.gene92301:CDS
TATAAACCCTTTGGAAGGTG+CGG 0.634750 5.1:+1070338 MS.gene92301:CDS
TGTAGTGTTTCTGTGTGGAA+TGG 0.635704 5.1:+1069271 MS.gene92301:CDS
GCATTGGCAGGAAGTACCAT+CGG 0.637875 5.1:+1069919 MS.gene92301:CDS
AGGATGCCAATGGTTGATGA+TGG 0.639984 5.1:+1070777 MS.gene92301:CDS
GGATCCAGGCATCACAACAC+CGG 0.644727 5.1:-1070724 None:intergenic
GATATGCAAGGTTGTTGACA+GGG 0.644837 5.1:+1070128 MS.gene92301:CDS
ATACTTGTGTACGAAGGTGA+GGG 0.646011 5.1:+1070573 MS.gene92301:CDS
AGGATGTTCTTATATCCCAA+GGG 0.646556 5.1:+1070266 MS.gene92301:CDS
GCTCGAATTCCAATGGCAAG+AGG 0.649760 5.1:-1070447 None:intergenic
TGGTTGATGATGGTCATGGT+TGG 0.651359 5.1:+1070787 MS.gene92301:CDS
CAAACGACCGATAAGAAATG+TGG 0.653357 5.1:+1069639 MS.gene92301:CDS
TGACAGTGAAACAACTGGGG+AGG 0.653527 5.1:+1069192 MS.gene92301:CDS
ACTCCACCAGAAACATTGCA+AGG 0.653765 5.1:-1069591 None:intergenic
GCCTGGATCCCGTATACCTG+TGG 0.656774 5.1:+1070737 MS.gene92301:CDS
GTTTGAGGAGGTGAACAATG+AGG 0.660540 5.1:+1070155 MS.gene92301:CDS
CCAATGGTTGATGATGGTCA+TGG 0.660629 5.1:+1070783 MS.gene92301:CDS
ATGTTCTTATATCCCAAGGG+TGG 0.662483 5.1:+1070269 MS.gene92301:CDS
TGTGTGGAATGGCTCCGAAG+TGG 0.667993 5.1:+1069282 MS.gene92301:CDS
TGACTGGTAACTCCTCCAGA+AGG 0.672347 5.1:-1069370 None:intergenic
GAGGGTAACCAAATCCATTG+TGG 0.675212 5.1:-1070841 None:intergenic
GATGCCTGAACCAACAGCAG+TGG 0.675835 5.1:+1069750 MS.gene92301:CDS
CAGAGTTGATACTGACCCTG+TGG 0.678557 5.1:+1069456 MS.gene92301:CDS
TATTCATGATAATCAAACTG+AGG 0.679559 5.1:+1070545 MS.gene92301:CDS
GCTGTGACTGCTACCGCCGG+GGG 0.681642 5.1:+1069880 MS.gene92301:CDS
AAATATATTCAAGCGTCATG+GGG 0.682975 5.1:+1069993 MS.gene92301:CDS
AGATGGTTTGAAGATATGCA+AGG 0.687926 5.1:+1070116 MS.gene92301:CDS
TTGGCAGGAAGTACCATCGG+TGG 0.690515 5.1:+1069922 MS.gene92301:CDS
CACAATGTAGTGTTTCTGTG+TGG 0.703064 5.1:+1069266 MS.gene92301:CDS
ATGCAAGGTTGTTGACAGGG+CGG 0.708122 5.1:+1070131 MS.gene92301:CDS
TATGGAAGTGAGGATGCCAA+TGG 0.723950 5.1:+1070767 MS.gene92301:CDS
ATCTGACAGTGAAACAACTG+GGG 0.741112 5.1:+1069189 MS.gene92301:CDS
GCATTGATACTTGTGTACGA+AGG 0.792141 5.1:+1070567 MS.gene92301:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAACAGAAGTTTATAA+AGG + chr5.1:1070315-1070334 MS.gene92301:CDS 15.0%
!! TTAGGATATTTCAAGATAAT+GGG + chr5.1:1070621-1070640 MS.gene92301:CDS 20.0%
!!! TTGAATATATTTTCAGAATC+TGG - chr5.1:1069985-1070004 None:intergenic 20.0%
! AAAATATATTCAAGCGTCAT+GGG + chr5.1:1069992-1070011 MS.gene92301:CDS 25.0%
! ATAAATGATGTGATACTTGT+TGG + chr5.1:1070243-1070262 MS.gene92301:CDS 25.0%
! GGTCAAAGAAATTAAATCAA+TGG + chr5.1:1070014-1070033 MS.gene92301:CDS 25.0%
! GTTAGGATATTTCAAGATAA+TGG + chr5.1:1070620-1070639 MS.gene92301:CDS 25.0%
! TATTCATGATAATCAAACTG+AGG + chr5.1:1070545-1070564 MS.gene92301:CDS 25.0%
!! AATTGCTCTCATTTTCAATA+TGG + chr5.1:1069837-1069856 MS.gene92301:CDS 25.0%
!! ATTGCTCTCATTTTCAATAT+GGG + chr5.1:1069838-1069857 MS.gene92301:CDS 25.0%
!! TTATAAAGGTATAAACCCTT+TGG + chr5.1:1070329-1070348 MS.gene92301:CDS 25.0%
!!! AACTTCTGTTATTTTGCATA+AGG - chr5.1:1070310-1070329 None:intergenic 25.0%
AAAGCTTCTCAAGAAACTAT+GGG + chr5.1:1069799-1069818 MS.gene92301:CDS 30.0%
AAATATATTCAAGCGTCATG+GGG + chr5.1:1069993-1070012 MS.gene92301:CDS 30.0%
ATAGCGATTGGAATTGATAT+TGG + chr5.1:1069238-1069257 MS.gene92301:CDS 30.0%
CATGTAAACATTGGATGATA+AGG - chr5.1:1070200-1070219 None:intergenic 30.0%
GAAAATATATTCAAGCGTCA+TGG + chr5.1:1069991-1070010 MS.gene92301:CDS 30.0%
TCTTGAAATATCCTAACAAG+TGG - chr5.1:1070617-1070636 None:intergenic 30.0%
TTCAATATGAAACGCTTGAT+CGG + chr5.1:1069433-1069452 MS.gene92301:CDS 30.0%
! AAGTAAAAATCTGCCATTTC+TGG + chr5.1:1069486-1069505 MS.gene92301:CDS 30.0%
AAAAGTAGATCTTTACGTGC+TGG - chr5.1:1070519-1070538 None:intergenic 35.0%
AACAAGTCCAAGTTTCCAAA+GGG - chr5.1:1070411-1070430 None:intergenic 35.0%
AGATGGTTTGAAGATATGCA+AGG + chr5.1:1070116-1070135 MS.gene92301:CDS 35.0%
AGGATGTTCTTATATCCCAA+GGG + chr5.1:1070266-1070285 MS.gene92301:CDS 35.0%
AGTTTGTACCTGTAATTCCA+AGG + chr5.1:1070481-1070500 MS.gene92301:CDS 35.0%
ATGATGCAAAGGTAGAAGTT+GGG + chr5.1:1070217-1070236 MS.gene92301:CDS 35.0%
CCAATGTTTACATGATGCAA+AGG + chr5.1:1070206-1070225 MS.gene92301:CDS 35.0%
CCTTTGCATCATGTAAACAT+TGG - chr5.1:1070209-1070228 None:intergenic 35.0%
GAAAGCTTCTCAAGAAACTA+TGG + chr5.1:1069798-1069817 MS.gene92301:CDS 35.0%
GTAAGCACCACATTTCTTAT+CGG - chr5.1:1069649-1069668 None:intergenic 35.0%
TAACAAGTCCAAGTTTCCAA+AGG - chr5.1:1070412-1070431 None:intergenic 35.0%
TTCACTAACGCAGCAATAAA+TGG - chr5.1:1069538-1069557 None:intergenic 35.0%
! AAAGGTATAAACCCTTTGGA+AGG + chr5.1:1070333-1070352 MS.gene92301:CDS 35.0%
! AATGATGTGATACTTGTTGG+AGG + chr5.1:1070246-1070265 MS.gene92301:CDS 35.0%
! AGTATCCCAGATAAAAGCAT+TGG + chr5.1:1069903-1069922 MS.gene92301:CDS 35.0%
! TCAATTCCAATCGCTATTTC+AGG - chr5.1:1069235-1069254 None:intergenic 35.0%
! TTTTACTTGCATGAACCACA+GGG - chr5.1:1069474-1069493 None:intergenic 35.0%
! TTTTTACTTGCATGAACCAC+AGG - chr5.1:1069475-1069494 None:intergenic 35.0%
!! GTTCAGGTTGATGTAGATTT+GGG + chr5.1:1070093-1070112 MS.gene92301:CDS 35.0%
!! GTTTTTCAAAAGCTCCACTT+CGG - chr5.1:1069299-1069318 None:intergenic 35.0%
!! TGTTCAGGTTGATGTAGATT+TGG + chr5.1:1070092-1070111 MS.gene92301:CDS 35.0%
AAAGCTGATGAATTGCATCC+TGG - chr5.1:1070059-1070078 None:intergenic 40.0%
AAATGTGGTGCTTACTGTTC+CGG + chr5.1:1069654-1069673 MS.gene92301:CDS 40.0%
AACATTGCAAGGACTTCTTC+TGG - chr5.1:1069583-1069602 None:intergenic 40.0%
AAGCTGATGAATTGCATCCT+GGG - chr5.1:1070058-1070077 None:intergenic 40.0%
ACAGCAGTGGCGTTATTATA+TGG + chr5.1:1069763-1069782 MS.gene92301:CDS 40.0%
AGAAGTCCTTGCAATGTTTC+TGG + chr5.1:1069585-1069604 MS.gene92301:CDS 40.0%
AGATATGCAAGGTTGTTGAC+AGG + chr5.1:1070127-1070146 MS.gene92301:CDS 40.0%
ATACTTGTGTACGAAGGTGA+GGG + chr5.1:1070573-1070592 MS.gene92301:CDS 40.0%
ATCAACCTGAACACTGCTTT+GGG - chr5.1:1070085-1070104 None:intergenic 40.0%
ATCTGACAGTGAAACAACTG+GGG + chr5.1:1069189-1069208 MS.gene92301:CDS 40.0%
ATGTTCTTATATCCCAAGGG+TGG + chr5.1:1070269-1070288 MS.gene92301:CDS 40.0%
CAAACGACCGATAAGAAATG+TGG + chr5.1:1069639-1069658 MS.gene92301:CDS 40.0%
CACAGAAACACTACATTGTG+AGG - chr5.1:1069266-1069285 None:intergenic 40.0%
CATCTGACAGTGAAACAACT+GGG + chr5.1:1069188-1069207 MS.gene92301:CDS 40.0%
CATGATGCAAAGGTAGAAGT+TGG + chr5.1:1070216-1070235 MS.gene92301:CDS 40.0%
GAGGATGTTCTTATATCCCA+AGG + chr5.1:1070265-1070284 MS.gene92301:CDS 40.0%
GATATGCAAGGTTGTTGACA+GGG + chr5.1:1070128-1070147 MS.gene92301:CDS 40.0%
GCAGAAGAAAACCACTTGTT+AGG + chr5.1:1070603-1070622 MS.gene92301:CDS 40.0%
GTTGATGTAGATTTGGGAGA+TGG + chr5.1:1070099-1070118 MS.gene92301:CDS 40.0%
TAACAAGATTCTCCACCCTT+GGG - chr5.1:1070284-1070303 None:intergenic 40.0%
TATAATAACGCCACTGCTGT+TGG - chr5.1:1069763-1069782 None:intergenic 40.0%
TATCTCCAAACAGCATCTCA+TGG - chr5.1:1069408-1069427 None:intergenic 40.0%
TGATGCAAAGGTAGAAGTTG+GGG + chr5.1:1070218-1070237 MS.gene92301:CDS 40.0%
TGGAAACGACTGAATGAAAC+CGG - chr5.1:1069676-1069695 None:intergenic 40.0%
TGTTTCACTGTCAGATGCAA+CGG - chr5.1:1069185-1069204 None:intergenic 40.0%
! CACAATGTAGTGTTTCTGTG+TGG + chr5.1:1069266-1069285 MS.gene92301:CDS 40.0%
! CTCATTTTCAATATGGGTGC+TGG + chr5.1:1069844-1069863 MS.gene92301:CDS 40.0%
! CTGATTTCAGGAACTCCTTT+TGG - chr5.1:1070660-1070679 None:intergenic 40.0%
! GCATTGATACTTGTGTACGA+AGG + chr5.1:1070567-1070586 MS.gene92301:CDS 40.0%
! TATAAACCCTTTGGAAGGTG+CGG + chr5.1:1070338-1070357 MS.gene92301:CDS 40.0%
! TCCTGCCAATGCTTTTATCT+GGG - chr5.1:1069911-1069930 None:intergenic 40.0%
! TGTAGTGTTTCTGTGTGGAA+TGG + chr5.1:1069271-1069290 MS.gene92301:CDS 40.0%
! TTCCTGCCAATGCTTTTATC+TGG - chr5.1:1069912-1069931 None:intergenic 40.0%
AAACAGCATCTCATGGTCGT+GGG - chr5.1:1069401-1069420 None:intergenic 45.0%
AACCAAATCTTCTCCACCGA+TGG - chr5.1:1069938-1069957 None:intergenic 45.0%
AATTCCAATGGCAAGAGGAG+TGG - chr5.1:1070445-1070464 None:intergenic 45.0%
ACTCCACCAGAAACATTGCA+AGG - chr5.1:1069594-1069613 None:intergenic 45.0%
CAAATCCATTGTGGCACCAT+AGG - chr5.1:1070835-1070854 None:intergenic 45.0%
CATCAACCTGAACACTGCTT+TGG - chr5.1:1070086-1070105 None:intergenic 45.0%
CCATGACCATCATCAACCAT+TGG - chr5.1:1070786-1070805 None:intergenic 45.0%
CCATTGGAATTCGAGCTGAT+GGG + chr5.1:1070454-1070473 MS.gene92301:CDS 45.0%
CCTCTTCCTGAAATAGCGAT+TGG + chr5.1:1069226-1069245 MS.gene92301:CDS 45.0%
CTGCGTTAGTGAACAATGTC+TGG + chr5.1:1069545-1069564 MS.gene92301:CDS 45.0%
GAGGGTAACCAAATCCATTG+TGG - chr5.1:1070844-1070863 None:intergenic 45.0%
GATACTTGTGTACGAAGGTG+AGG + chr5.1:1070572-1070591 MS.gene92301:CDS 45.0%
GCATCTGACAGTGAAACAAC+TGG + chr5.1:1069187-1069206 MS.gene92301:CDS 45.0%
GGCACCATAGGTCCTATTTA+GGG - chr5.1:1070823-1070842 None:intergenic 45.0%
GTAACAAGATTCTCCACCCT+TGG - chr5.1:1070285-1070304 None:intergenic 45.0%
GTGGTGTTCCTTGGAATTAC+AGG - chr5.1:1070492-1070511 None:intergenic 45.0%
GTTTGAGGAGGTGAACAATG+AGG + chr5.1:1070155-1070174 MS.gene92301:CDS 45.0%
TAACAGGCACCACAGGTATA+CGG - chr5.1:1070749-1070768 None:intergenic 45.0%
TACCATCGGTGGAGAAGATT+TGG + chr5.1:1069933-1069952 MS.gene92301:CDS 45.0%
TATACCTGTGGTGCCTGTTA+TGG + chr5.1:1070749-1070768 MS.gene92301:CDS 45.0%
TATGGAAGTGAGGATGCCAA+TGG + chr5.1:1070767-1070786 MS.gene92301:CDS 45.0%
TCAGATGCAACGGTATATGC+AGG - chr5.1:1069175-1069194 None:intergenic 45.0%
TCCTGAAATCAGTGTGTGCA+TGG + chr5.1:1070668-1070687 MS.gene92301:CDS 45.0%
TCTCAAGAAACTATGGGCAG+TGG + chr5.1:1069805-1069824 MS.gene92301:CDS 45.0%
TGGCACCATAGGTCCTATTT+AGG - chr5.1:1070824-1070843 None:intergenic 45.0%
TTCAAAGACGAAGCTCCTTC+TGG + chr5.1:1069355-1069374 MS.gene92301:CDS 45.0%
! ATCTTTACGTGCTGGCATTG+TGG - chr5.1:1070511-1070530 None:intergenic 45.0%
! CAGTGATCCCTTTGGAAACT+TGG + chr5.1:1070401-1070420 MS.gene92301:CDS 45.0%
! CCAATCGCTATTTCAGGAAG+AGG - chr5.1:1069229-1069248 None:intergenic 45.0%
! GGATTTGGTTACCCTCAAGA+AGG + chr5.1:1070848-1070867 MS.gene92301:CDS 45.0%
!! AGGATGCCAATGGTTGATGA+TGG + chr5.1:1070777-1070796 MS.gene92301:CDS 45.0%
!! AGTCCTTGCAATGTTTCTGG+TGG + chr5.1:1069588-1069607 MS.gene92301:CDS 45.0%
!! CCAATGGTTGATGATGGTCA+TGG + chr5.1:1070783-1070802 MS.gene92301:CDS 45.0%
!! CTATGGTGCCACAATGGATT+TGG + chr5.1:1070833-1070852 MS.gene92301:CDS 45.0%
!! TCCATGCACACACTGATTTC+AGG - chr5.1:1070672-1070691 None:intergenic 45.0%
!! TCCCAGATAAAAGCATTGGC+AGG + chr5.1:1069907-1069926 MS.gene92301:CDS 45.0%
!! TGGTTGATGATGGTCATGGT+TGG + chr5.1:1070787-1070806 MS.gene92301:CDS 45.0%
!! TTTTATCTGGGATACTCCCC+CGG - chr5.1:1069899-1069918 None:intergenic 45.0%
AAAGACGAAGCTCCTTCTGG+AGG + chr5.1:1069358-1069377 MS.gene92301:CDS 50.0%
AACAGGCACCACAGGTATAC+GGG - chr5.1:1070748-1070767 None:intergenic 50.0%
ACTTCCATAACAGGCACCAC+AGG - chr5.1:1070756-1070775 None:intergenic 50.0%
ATGCAAGGTTGTTGACAGGG+CGG + chr5.1:1070131-1070150 MS.gene92301:CDS 50.0%
ATGGTCGTGGGAGAATTGAC+TGG - chr5.1:1069389-1069408 None:intergenic 50.0%
CAAACAGCATCTCATGGTCG+TGG - chr5.1:1069402-1069421 None:intergenic 50.0%
CACAAACCGCACCTTCCAAA+GGG - chr5.1:1070347-1070366 None:intergenic 50.0%
CACGACCATGAGATGCTGTT+TGG + chr5.1:1069400-1069419 MS.gene92301:CDS 50.0%
CCCATCAGCTCGAATTCCAA+TGG - chr5.1:1070457-1070476 None:intergenic 50.0%
GAGGCCCTAAATAGGACCTA+TGG + chr5.1:1070816-1070835 MS.gene92301:CDS 50.0%
GCCATTGGAATTCGAGCTGA+TGG + chr5.1:1070453-1070472 MS.gene92301:CDS 50.0%
GCTCGAATTCCAATGGCAAG+AGG - chr5.1:1070450-1070469 None:intergenic 50.0%
GGAATACCTGCAGCACCAAA+AGG + chr5.1:1070642-1070661 MS.gene92301:CDS 50.0%
GGCATCCTCACTTCCATAAC+AGG - chr5.1:1070765-1070784 None:intergenic 50.0%
GTACGAAGGTGAGGGAAAGA+AGG + chr5.1:1070581-1070600 MS.gene92301:CDS 50.0%
GTTTGTGGTGCAGCAATGGA+AGG + chr5.1:1070360-1070379 MS.gene92301:CDS 50.0%
TCAGGCATCAGTGATCCCTT+TGG + chr5.1:1070393-1070412 MS.gene92301:CDS 50.0%
TCCAAGGTCTGCACCAGAAA+TGG - chr5.1:1069502-1069521 None:intergenic 50.0%
TGACAGTGAAACAACTGGGG+AGG + chr5.1:1069192-1069211 MS.gene92301:CDS 50.0%
TGACTGGTAACTCCTCCAGA+AGG - chr5.1:1069373-1069392 None:intergenic 50.0%
TGGTGCCTGTTATGGAAGTG+AGG + chr5.1:1070757-1070776 MS.gene92301:CDS 50.0%
TTCCACCCAAAGCAGTGTTC+AGG + chr5.1:1070077-1070096 MS.gene92301:CDS 50.0%
! AACCTGAACACTGCTTTGGG+TGG - chr5.1:1070082-1070101 None:intergenic 50.0%
! ATGGAAGGAGCTGTTGCTTC+AGG + chr5.1:1070375-1070394 MS.gene92301:CDS 50.0%
! TCGCACAAGCACGCTCTAAA+CGG - chr5.1:1069705-1069724 None:intergenic 50.0%
! TGCAAGGACTTCTTCTGGAG+TGG - chr5.1:1069578-1069597 None:intergenic 50.0%
! TTTAGAGCGTGCTTGTGCGA+TGG + chr5.1:1069705-1069724 MS.gene92301:CDS 50.0%
!! AACGTGCTGCGAGTTTTAGC+CGG + chr5.1:1070705-1070724 MS.gene92301:CDS 50.0%
!! AAGGACTTCTTCTGGAGTGG+TGG - chr5.1:1069575-1069594 None:intergenic 50.0%
!! CAGAGTTGATACTGACCCTG+TGG + chr5.1:1069456-1069475 MS.gene92301:CDS 50.0%
!! GCATTGGCAGGAAGTACCAT+CGG + chr5.1:1069919-1069938 MS.gene92301:CDS 50.0%
!! TAGGACCTATGGTGCCACAA+TGG + chr5.1:1070827-1070846 MS.gene92301:CDS 50.0%
!! TGGCTGGTCTTCATGTTCTC+AGG + chr5.1:1069725-1069744 MS.gene92301:CDS 50.0%
ACCACAGGTATACGGGATCC+AGG - chr5.1:1070741-1070760 None:intergenic 55.0%
CAAGCCACTCCTCTTGCCAT+TGG + chr5.1:1070438-1070457 MS.gene92301:CDS 55.0%
CCACAAACCGCACCTTCCAA+AGG - chr5.1:1070348-1070367 None:intergenic 55.0%
CTGGTGCAGACCTTGGACAT+TGG + chr5.1:1069505-1069524 MS.gene92301:CDS 55.0%
GATGCCTGAACCAACAGCAG+TGG + chr5.1:1069750-1069769 MS.gene92301:CDS 55.0%
GCTGGCATTGTGGTGTTCCT+TGG - chr5.1:1070501-1070520 None:intergenic 55.0%
GGATCCAGGCATCACAACAC+CGG - chr5.1:1070727-1070746 None:intergenic 55.0%
GGTGTGCTGAGGCCCTAAAT+AGG + chr5.1:1070808-1070827 MS.gene92301:CDS 55.0%
TGCGGTTTGTGGTGCAGCAA+TGG + chr5.1:1070356-1070375 MS.gene92301:CDS 55.0%
TGGACGAACGCCAATGTCCA+AGG - chr5.1:1069518-1069537 None:intergenic 55.0%
TGTGTGGAATGGCTCCGAAG+TGG + chr5.1:1069282-1069301 MS.gene92301:CDS 55.0%
TGTTGACAGGGCGGAGTTTG+AGG + chr5.1:1070140-1070159 MS.gene92301:CDS 55.0%
! CCTTTGGAAGGTGCGGTTTG+TGG + chr5.1:1070345-1070364 MS.gene92301:CDS 55.0%
! CGCACAAGCACGCTCTAAAC+GGG - chr5.1:1069704-1069723 None:intergenic 55.0%
! TGGTCATGGTTGGTGTGCTG+AGG + chr5.1:1070797-1070816 MS.gene92301:CDS 55.0%
!! AACGCCACTGCTGTTGGTTC+AGG - chr5.1:1069757-1069776 None:intergenic 55.0%
!! ATTGCTTCGAGTTGCAGCCC+AGG + chr5.1:1070038-1070057 MS.gene92301:CDS 55.0%
!! GCCATTTCTGGTGCAGACCT+TGG + chr5.1:1069498-1069517 MS.gene92301:CDS 55.0%
!! TTAGCCGGTGTTGTGATGCC+TGG + chr5.1:1070720-1070739 MS.gene92301:CDS 55.0%
!! TTGGCAGGAAGTACCATCGG+TGG + chr5.1:1069922-1069941 MS.gene92301:CDS 55.0%
!!! GGAACTCCTTTTGGTGCTGC+AGG - chr5.1:1070651-1070670 None:intergenic 55.0%
GCCTGGATCCCGTATACCTG+TGG + chr5.1:1070737-1070756 MS.gene92301:CDS 60.0%
GTTGCTGTGACTGCTACCGC+CGG + chr5.1:1069877-1069896 MS.gene92301:CDS 60.0%
TGACAGGGCGGAGTTTGAGG+AGG + chr5.1:1070143-1070162 MS.gene92301:CDS 60.0%
TTGCTGTGACTGCTACCGCC+GGG + chr5.1:1069878-1069897 MS.gene92301:CDS 60.0%
! CACGCTCTAAACGGGTCAGC+TGG - chr5.1:1069696-1069715 None:intergenic 60.0%
! TATCTGGGATACTCCCCCGG+CGG - chr5.1:1069896-1069915 None:intergenic 60.0%
GAGCGTGCTTGTGCGATGGC+TGG + chr5.1:1069709-1069728 MS.gene92301:CDS 65.0%
TGCTGTGACTGCTACCGCCG+GGG + chr5.1:1069879-1069898 MS.gene92301:CDS 65.0%
GCTGTGACTGCTACCGCCGG+GGG + chr5.1:1069880-1069899 MS.gene92301:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 1069157 1070875 1069157 ID=MS.gene92301
chr5.1 mRNA 1069157 1070875 1069157 ID=MS.gene92301.t1;Parent=MS.gene92301
chr5.1 exon 1069157 1070875 1069157 ID=MS.gene92301.t1.exon1;Parent=MS.gene92301.t1
chr5.1 CDS 1069157 1070875 1069157 ID=cds.MS.gene92301.t1;Parent=MS.gene92301.t1
Gene Sequence

>MS.gene92301

ATGGCAGAACGAGAACCTGCATATACCGTTGCATCTGACAGTGAAACAACTGGGGAGGAAAAATCATCACCTCTTCCTGAAATAGCGATTGGAATTGATATTGGCACCTCACAATGTAGTGTTTCTGTGTGGAATGGCTCCGAAGTGGAGCTTTTGAAAAACAAAAGAAATCAGAAGCTTATGAAGTCATTTGTGACTTTCAAAGACGAAGCTCCTTCTGGAGGAGTTACCAGTCAATTCTCCCACGACCATGAGATGCTGTTTGGAGATACAATTTTCAATATGAAACGCTTGATCGGCAGAGTTGATACTGACCCTGTGGTTCATGCAAGTAAAAATCTGCCATTTCTGGTGCAGACCTTGGACATTGGCGTTCGTCCATTTATTGCTGCGTTAGTGAACAATGTCTGGAGATCCACCACTCCAGAAGAAGTCCTTGCAATGTTTCTGGTGGAGTTAAGACTGATGACTGAAACTCATCTCAAACGACCGATAAGAAATGTGGTGCTTACTGTTCCGGTTTCATTCAGTCGTTTCCAGCTGACCCGTTTAGAGCGTGCTTGTGCGATGGCTGGTCTTCATGTTCTCAGGTTGATGCCTGAACCAACAGCAGTGGCGTTATTATATGGACAACAACAACAGAAAGCTTCTCAAGAAACTATGGGCAGTGGAAGTGAGAAAATTGCTCTCATTTTCAATATGGGTGCTGGTTATTGTGATGTTGCTGTGACTGCTACCGCCGGGGGAGTATCCCAGATAAAAGCATTGGCAGGAAGTACCATCGGTGGAGAAGATTTGGTTCAGAATATGATGTGTTATCTCTTACCAGATTCTGAAAATATATTCAAGCGTCATGGGGTCAAAGAAATTAAATCAATGGCATTGCTTCGAGTTGCAGCCCAGGATGCAATTCATCAGCTTTCCACCCAAAGCAGTGTTCAGGTTGATGTAGATTTGGGAGATGGTTTGAAGATATGCAAGGTTGTTGACAGGGCGGAGTTTGAGGAGGTGAACAATGAGGTGTTCGAAAAGTGTGAAAGCCTTATCATCCAATGTTTACATGATGCAAAGGTAGAAGTTGGGGATATAAATGATGTGATACTTGTTGGAGGATGTTCTTATATCCCAAGGGTGGAGAATCTTGTTACAACCTTATGCAAAATAACAGAAGTTTATAAAGGTATAAACCCTTTGGAAGGTGCGGTTTGTGGTGCAGCAATGGAAGGAGCTGTTGCTTCAGGCATCAGTGATCCCTTTGGAAACTTGGACTTGTTAACGATTCAAGCCACTCCTCTTGCCATTGGAATTCGAGCTGATGGGAACAAGTTTGTACCTGTAATTCCAAGGAACACCACAATGCCAGCACGTAAAGATCTACTTTTCACAACTATTCATGATAATCAAACTGAGGCATTGATACTTGTGTACGAAGGTGAGGGAAAGAAGGCAGAAGAAAACCACTTGTTAGGATATTTCAAGATAATGGGAATACCTGCAGCACCAAAAGGAGTTCCTGAAATCAGTGTGTGCATGGACATAGATGCTGCAAACGTGCTGCGAGTTTTAGCCGGTGTTGTGATGCCTGGATCCCGTATACCTGTGGTGCCTGTTATGGAAGTGAGGATGCCAATGGTTGATGATGGTCATGGTTGGTGTGCTGAGGCCCTAAATAGGACCTATGGTGCCACAATGGATTTGGTTACCCTCAAGAAGGCATAA
Protein sequence

>MS.gene92301.t1

MAEREPAYTVASDSETTGEEKSSPLPEIAIGIDIGTSQCSVSVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQFSHDHEMLFGDTIFNMKRLIGRVDTDPVVHASKNLPFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKASQETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGSTIGGEDLVQNMMCYLLPDSENIFKRHGVKEIKSMALLRVAAQDAIHQLSTQSSVQVDVDLGDGLKICKVVDRAEFEEVNNEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIPRVENLVTTLCKITEVYKGINPLEGAVCGAAMEGAVASGISDPFGNLDLLTIQATPLAIGIRADGNKFVPVIPRNTTMPARKDLLFTTIHDNQTEALILVYEGEGKKAEENHLLGYFKIMGIPAAPKGVPEISVCMDIDAANVLRVLAGVVMPGSRIPVVPVMEVRMPMVDDGHGWCAEALNRTYGATMDLVTLKKA