AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94205

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94205.t1 MTR_5g083360 98.589 567 8 0 1 567 1 567 0.0 1163
MS.gene94205.t1 MTR_3g071890 81.679 524 85 3 4 523 10 526 0.0 887
MS.gene94205.t1 MTR_5g073210 59.331 568 222 4 1 567 1 560 0.0 727
MS.gene94205.t1 MTR_5g020620 50.619 565 259 5 7 567 13 561 0.0 606
MS.gene94205.t1 MTR_5g020600 51.068 562 253 7 12 567 21 566 0.0 593
MS.gene94205.t1 MTR_7g062250 52.014 571 255 9 7 567 17 578 0.0 589
MS.gene94205.t1 MTR_2g008330 48.866 573 280 6 1 567 14 579 0.0 586
MS.gene94205.t1 MTR_7g058690 51.872 561 253 10 17 567 27 580 0.0 582
MS.gene94205.t1 MTR_7g062310 51.630 552 252 8 25 567 48 593 0.0 582
MS.gene94205.t1 MTR_5g069680 49.381 565 266 7 7 567 10 558 0.0 581
MS.gene94205.t1 MTR_4g064530 52.285 547 245 9 12 551 32 569 0.0 578
MS.gene94205.t1 MTR_4g015120 49.562 571 270 6 1 567 1 557 0.0 575
MS.gene94205.t1 MTR_7g458880 49.739 575 269 10 6 567 13 580 0.0 573
MS.gene94205.t1 MTR_3g101635 49.731 557 263 7 2 553 8 552 0.0 568
MS.gene94205.t1 MTR_7g060460 50.177 566 258 11 16 567 26 581 0.0 567
MS.gene94205.t1 MTR_3g101640 48.103 580 279 8 1 567 1 571 0.0 563
MS.gene94205.t1 MTR_3g462760 49.094 552 260 8 20 567 22 556 0.0 557
MS.gene94205.t1 MTR_5g081810 50.841 535 242 6 37 567 12 529 0.0 554
MS.gene94205.t1 MTR_7g065970 49.223 579 273 9 1 567 1 570 0.0 546
MS.gene94205.t1 MTR_4g064530 51.423 527 240 9 12 531 32 549 0.0 538
MS.gene94205.t1 MTR_4g019225 49.101 556 260 8 25 567 27 572 0.0 535
MS.gene94205.t1 MTR_7g065980 45.899 573 284 10 10 567 10 571 0.0 533
MS.gene94205.t1 MTR_8g027375 44.786 585 291 6 1 567 1 571 4.27e-179 517
MS.gene94205.t1 MTR_8g091390 31.229 586 319 20 19 567 29 567 4.59e-72 241
MS.gene94205.t1 MTR_3g078730 29.701 569 345 13 7 564 8 532 2.57e-67 228
MS.gene94205.t1 MTR_3g078700 29.359 562 333 15 1 545 4 518 3.64e-66 224
MS.gene94205.t1 MTR_3g078740 31.293 588 332 20 21 564 23 582 1.27e-65 224
MS.gene94205.t1 MTR_5g081790 42.509 287 143 6 243 525 152 420 1.56e-65 220
MS.gene94205.t1 MTR_5g081790 48.795 166 75 2 8 163 5 170 2.47e-47 171
MS.gene94205.t1 MTR_8g479390 28.081 495 305 15 38 527 45 493 4.36e-42 159
MS.gene94205.t1 MTR_4g101650 29.026 534 333 18 7 527 9 509 2.05e-41 158
MS.gene94205.t1 MTR_3g008580 25.965 570 378 20 9 567 15 551 2.35e-39 152
MS.gene94205.t1 MTR_1g090707 26.703 543 341 13 38 567 38 536 2.94e-39 152
MS.gene94205.t1 MTR_4g049390 26.908 498 300 14 42 527 45 490 1.22e-37 146
MS.gene94205.t1 MTR_8g073840 39.572 187 74 4 17 202 23 171 2.86e-37 140
MS.gene94205.t1 MTR_8g479380 28.039 510 290 18 42 533 47 497 1.17e-36 144
MS.gene94205.t1 MTR_1g026410 25.852 499 316 13 42 533 47 498 2.45e-36 142
MS.gene94205.t1 MTR_5g018270 27.525 505 292 17 38 527 45 490 2.55e-36 142
MS.gene94205.t1 MTR_4g075690 25.000 564 372 15 8 567 16 532 4.28e-36 142
MS.gene94205.t1 MTR_3g115050 25.992 504 309 15 42 533 48 499 1.48e-35 140
MS.gene94205.t1 MTR_2g088080 26.735 490 311 12 42 527 48 493 1.01e-34 138
MS.gene94205.t1 MTR_4g048350 26.735 490 310 15 42 527 45 489 2.78e-34 137
MS.gene94205.t1 MTR_7g051440 27.011 522 326 17 27 532 32 514 8.02e-33 133
MS.gene94205.t1 MTR_3g083430 24.632 544 349 14 38 567 39 535 1.67e-31 129
MS.gene94205.t1 MTR_3g083390 24.449 544 350 14 38 567 39 535 2.99e-31 128
MS.gene94205.t1 MTR_3g083400 25.000 540 352 14 38 567 39 535 3.67e-31 127
MS.gene94205.t1 MTR_3g083450 24.954 541 351 16 38 567 40 536 3.70e-31 127
MS.gene94205.t1 MTR_3g083440 24.449 544 350 14 38 567 39 535 6.14e-31 127
MS.gene94205.t1 MTR_4g083210 24.953 537 343 17 11 533 7 497 1.21e-30 126
MS.gene94205.t1 MTR_7g058670 60.494 81 31 1 81 160 13 93 3.60e-30 114
MS.gene94205.t1 MTR_3g083410 24.813 536 350 14 42 567 7 499 4.28e-30 124
MS.gene94205.t1 MTR_4g075690 25.314 478 307 14 8 481 16 447 1.53e-29 121
MS.gene94205.t1 MTR_4g132320 26.177 531 334 20 7 527 12 494 2.74e-28 119
MS.gene94205.t1 MTR_4g083190 25.520 529 355 14 42 567 45 537 6.99e-28 118
MS.gene94205.t1 MTR_2g088080 25.277 451 289 12 81 527 6 412 4.29e-24 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94205.t1 AT2G40370 76.814 565 122 3 12 567 16 580 0.0 931
MS.gene94205.t1 AT5G05390 77.058 571 121 3 1 567 1 565 0.0 929
MS.gene94205.t1 AT2G30210 64.336 572 197 4 1 567 1 570 0.0 797
MS.gene94205.t1 AT5G07130 65.674 571 190 6 1 567 1 569 0.0 776
MS.gene94205.t1 AT5G07130 65.638 486 161 6 86 567 1 484 0.0 650
MS.gene94205.t1 AT5G07130 65.638 486 161 6 86 567 1 484 0.0 650
MS.gene94205.t1 AT5G09360 51.228 570 263 7 1 567 12 569 0.0 602
MS.gene94205.t1 AT2G29130 51.207 580 250 11 7 567 8 573 0.0 588
MS.gene94205.t1 AT5G60020 49.654 578 269 11 7 567 5 577 0.0 568
MS.gene94205.t1 AT5G03260 49.209 569 264 7 8 567 5 557 0.0 568
MS.gene94205.t1 AT2G38080 48.057 566 272 8 7 567 10 558 0.0 566
MS.gene94205.t1 AT5G60020 51.188 547 248 10 35 567 4 545 0.0 563
MS.gene94205.t1 AT3G09220 50.091 549 254 7 11 550 13 550 0.0 561
MS.gene94205.t1 AT5G01190 49.912 565 263 6 9 567 8 558 0.0 560
MS.gene94205.t1 AT5G58910 46.573 569 283 10 6 567 13 567 0.0 546
MS.gene94205.t1 AT5G01190 51.423 527 238 5 45 567 32 544 0.0 543
MS.gene94205.t1 AT5G58910 46.479 568 284 9 6 567 13 566 0.0 543
MS.gene94205.t1 AT5G48100 47.016 553 277 9 4 551 6 547 0.0 534
MS.gene94205.t1 AT2G46570 45.645 574 285 6 7 567 10 569 0.0 531
MS.gene94205.t1 AT1G18140 48.301 559 262 12 27 567 32 581 0.0 524
MS.gene94205.t1 AT5G01040 45.724 573 285 9 5 567 10 566 0.0 522
MS.gene94205.t1 AT5G58910 46.580 541 264 9 33 567 2 523 8.87e-180 517
MS.gene94205.t1 AT5G60020 49.440 536 249 11 7 525 5 535 1.21e-179 518
MS.gene94205.t1 AT5G01050 45.565 575 285 8 5 567 10 568 5.62e-179 518
MS.gene94205.t1 AT2G46570 46.667 495 242 5 81 567 4 484 1.91e-163 474
MS.gene94205.t1 AT3G09220 50.647 464 209 7 96 550 1 453 2.86e-163 473
MS.gene94205.t1 AT5G21100 31.434 579 325 20 19 564 19 558 1.44e-73 245
MS.gene94205.t1 AT4G39830 30.757 608 339 22 1 567 11 577 5.32e-71 239
MS.gene94205.t1 AT5G21105 30.442 588 344 18 7 564 21 573 2.31e-69 234
MS.gene94205.t1 AT5G21105 29.872 549 324 17 7 525 21 538 1.98e-59 207
MS.gene94205.t1 AT4G39830 29.942 521 286 20 85 567 3 482 2.48e-51 183
MS.gene94205.t1 AT4G39830 29.808 520 286 20 86 567 2 480 1.53e-50 181
MS.gene94205.t1 AT4G39830 29.287 519 279 18 85 567 3 469 1.54e-48 176
MS.gene94205.t1 AT5G66920 27.017 533 333 16 42 567 55 538 2.98e-43 162
MS.gene94205.t1 AT4G12420 27.586 551 341 20 1 532 1 512 3.17e-43 163
MS.gene94205.t1 AT4G12420 27.586 551 341 20 1 532 1 512 3.17e-43 163
MS.gene94205.t1 AT1G75790 26.577 523 343 12 8 527 11 495 1.40e-41 157
MS.gene94205.t1 AT5G51480 26.418 564 368 16 17 567 21 550 7.41e-41 156
MS.gene94205.t1 AT1G21860 25.898 529 338 15 11 533 16 496 1.49e-38 149
MS.gene94205.t1 AT1G76160 24.811 528 354 14 7 533 11 496 3.45e-38 148
MS.gene94205.t1 AT4G25240 26.562 576 371 18 8 567 11 550 1.55e-36 144
MS.gene94205.t1 AT4G25240 26.562 576 371 18 8 567 11 550 1.55e-36 144
MS.gene94205.t1 AT5G21105 29.781 366 215 11 208 564 50 382 2.17e-36 140
MS.gene94205.t1 AT1G41830 26.148 501 311 18 42 533 47 497 2.85e-35 139
MS.gene94205.t1 AT1G21850 25.836 538 336 18 7 533 11 496 3.48e-35 139
MS.gene94205.t1 AT5G48450 25.224 559 372 17 9 564 13 528 7.01e-34 136
MS.gene94205.t1 AT5G48450 25.224 559 372 17 9 564 13 528 1.18e-33 135
MS.gene94205.t1 AT4G22010 27.126 494 305 17 42 527 44 490 3.73e-32 130
MS.gene94205.t1 AT2G23630 27.239 536 329 17 42 567 48 532 8.59e-31 126
MS.gene94205.t1 AT2G23630 27.239 536 329 17 42 567 53 537 1.08e-30 126
MS.gene94205.t1 AT4G37160 26.261 575 358 16 7 567 12 534 1.73e-30 125
MS.gene94205.t1 AT1G55570 25.558 493 321 12 42 527 47 500 8.92e-29 120
MS.gene94205.t1 AT3G13390 25.490 510 334 13 25 527 29 499 2.81e-28 119
MS.gene94205.t1 AT1G55560 24.813 536 334 18 42 564 44 523 2.29e-27 116
MS.gene94205.t1 AT4G28090 23.944 497 325 15 45 533 49 500 2.48e-27 116
MS.gene94205.t1 AT4G37160 30.063 316 203 5 7 319 12 312 1.47e-24 107
MS.gene94205.t1 AT3G13400 25.251 499 314 15 42 527 45 497 1.57e-24 108
MS.gene94205.t1 AT3G13400 25.251 499 314 15 42 527 45 497 1.57e-24 108
MS.gene94205.t1 AT4G38420 23.279 494 329 15 42 527 48 499 4.33e-22 100
MS.gene94205.t1 AT4G38420 27.386 241 165 5 42 281 48 279 8.10e-20 92.8
MS.gene94205.t1 AT4G38420 27.386 241 165 5 42 281 48 279 1.36e-19 92.4

Find 141 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAAGCTTTAGAAGTTTA+AGG 0.083519 5.4:-66099281 MS.gene94205:CDS
ACCTATAGATTTACCATTAA+TGG 0.101466 5.4:-66100670 MS.gene94205:CDS
CACCTACTACTGTTAGTTTA+TGG 0.135494 5.4:+66099583 None:intergenic
TGTACCTTCTTGTCCATTAA+TGG 0.180702 5.4:+66100657 None:intergenic
TGGCTTAGAGCCACAGTTTA+TGG 0.186697 5.4:-66100610 MS.gene94205:CDS
CGTGAAACACCCATTCTTCT+AGG 0.253813 5.4:-66100532 MS.gene94205:intron
ACAATTGTTCCAATTGATTC+AGG 0.259031 5.4:-66099672 MS.gene94205:CDS
TATATTGTTGCTGAGGGATT+TGG 0.270036 5.4:-66098859 MS.gene94205:CDS
AAAGTTAATAAGTTGAGATT+TGG 0.271185 5.4:-66098964 MS.gene94205:CDS
ACTGTTGCTGTACCTGTTAA+TGG 0.271612 5.4:-66098775 MS.gene94205:CDS
TCTGATGCATACACTATTAA+TGG 0.273121 5.4:-66100109 MS.gene94205:CDS
CCAACTTTGGAAATTAATAA+TGG 0.283923 5.4:-66101545 MS.gene94205:CDS
GGTGGCATGCTCATAGTTCA+TGG 0.302145 5.4:-66100630 MS.gene94205:CDS
TGAGGACCTGCCTTTGTGTT+AGG 0.313647 5.4:-66098094 None:intergenic
GTGCAGCATTGATTACTCTT+AGG 0.316195 5.4:+66099637 None:intergenic
GTGTATGCATCAGATATATT+TGG 0.328938 5.4:+66100118 None:intergenic
GGACATTGAGTCACAAATTC+TGG 0.334936 5.4:+66100715 None:intergenic
AGATCTTCATCAATTTCATT+AGG 0.335973 5.4:+66099246 None:intergenic
AGAGGATGGAGAAGGGCTAT+TGG 0.338124 5.4:-66098136 MS.gene94205:CDS
ACAACCAATGTTCTCATGTT+AGG 0.341290 5.4:-66099534 MS.gene94205:CDS
TGTTCACATTGGATGGGGTT+TGG 0.346069 5.4:-66098175 MS.gene94205:CDS
GTCCTCAGGAGGAGCTTCTA+TGG 0.350489 5.4:+66098110 None:intergenic
GCTTGTCTCACAACATCAAT+TGG 0.351287 5.4:+66100160 None:intergenic
AATCACCCTATTCATCTTCA+TGG 0.351556 5.4:-66098892 MS.gene94205:CDS
TCATATCCATGAAGATGAAT+AGG 0.353366 5.4:+66098886 None:intergenic
CCATTATTAATTTCCAAAGT+TGG 0.356421 5.4:+66101545 None:intergenic
GTTTGATGTATGTAGGTGTT+TGG 0.364623 5.4:-66098218 MS.gene94205:intron
TTTGTTTCTCCTGAATCAAT+TGG 0.365718 5.4:+66099663 None:intergenic
CGCCGGGCGCGCTTACCAAT+CGG 0.373787 5.4:-66099451 MS.gene94205:CDS
GGATAAGCATCTCCTTCCTT+AGG 0.376333 5.4:+66100571 None:intergenic
AATTAATAATGGAGATACTT+TGG 0.377624 5.4:-66101534 MS.gene94205:CDS
GAGATTTGGATCAAGGGTTC+AGG 0.378810 5.4:-66098950 MS.gene94205:CDS
ACAATTTCCTGGTCCAACTT+TGG 0.400549 5.4:-66101558 MS.gene94205:CDS
ATAACTGCCCAACCATTAAC+AGG 0.400604 5.4:+66098763 None:intergenic
AGAATGGGTGTTTCACGCTT+TGG 0.403573 5.4:+66100538 None:intergenic
TGCTGTACCTGTTAATGGTT+GGG 0.408222 5.4:-66098770 MS.gene94205:CDS
TCCATTAATGGTAAATCTAT+AGG 0.411836 5.4:+66100669 None:intergenic
CAATGACCGACTCACATTAC+CGG 0.413180 5.4:+66099008 None:intergenic
ATAAGTTGAGATTTGGATCA+AGG 0.414127 5.4:-66098957 MS.gene94205:CDS
AGCCGATTGGTAAGCGCGCC+CGG 0.414132 5.4:+66099449 None:intergenic
CCGGCGATGTAGTATCTTGA+TGG 0.420568 5.4:+66099468 None:intergenic
GTTACAATGTCACCATTCAT+TGG 0.426411 5.4:-66101487 MS.gene94205:intron
GGATGAGAACATCAGTGGTT+TGG 0.428167 5.4:+66099505 None:intergenic
ACTGTGAATGGACAATTTCC+TGG 0.436760 5.4:-66101569 MS.gene94205:CDS
ATCCTGCAGGCTCATAAACT+TGG 0.437751 5.4:-66099090 MS.gene94205:CDS
CTTGTCTCACAACATCAATT+GGG 0.442182 5.4:+66100161 None:intergenic
CTTGGATGTTCACATTGGAT+GGG 0.442663 5.4:-66098181 MS.gene94205:CDS
ATGATCATTACCTAGAAGAA+TGG 0.444961 5.4:+66100522 None:intergenic
GCAGGCAATGAAGGCATGAT+TGG 0.446630 5.4:+66099336 None:intergenic
TAACTTTCTCCAGGACGAAT+AGG 0.449717 5.4:+66100694 None:intergenic
ACTTGGATGTTCACATTGGA+TGG 0.450150 5.4:-66098182 MS.gene94205:CDS
CATATCCATGAAGATGAATA+GGG 0.455044 5.4:+66098887 None:intergenic
GTGAGACAAGCCACACAAAC+AGG 0.461238 5.4:-66100148 MS.gene94205:CDS
GTGTTGCACAGCCTCTTCAC+TGG 0.461801 5.4:+66101605 None:intergenic
ATTATTATTAATTAGGGGAA+TGG 0.462012 5.4:-66100195 MS.gene94205:intron
TCATTGTGGCAACCGATTCA+AGG 0.473488 5.4:-66098991 MS.gene94205:CDS
TCATCACCTTGTCCTGCAAA+GGG 0.474167 5.4:-66099375 MS.gene94205:CDS
TAACACAAAGGCAGGTCCTC+AGG 0.478994 5.4:+66098096 None:intergenic
ATTATTAATTAGGGGAATGG+TGG 0.479401 5.4:-66100192 MS.gene94205:intron
TTGCTGTACCTGTTAATGGT+TGG 0.479566 5.4:-66098771 MS.gene94205:CDS
CAAATGAGCATCACTGTGAA+TGG 0.479962 5.4:-66101581 MS.gene94205:CDS
TGATGATTTGTATTCAAGAA+TGG 0.480556 5.4:+66099392 None:intergenic
ATCAATGTCAAGGACCGAAC+GGG 0.480946 5.4:-66099170 MS.gene94205:CDS
TTAACTTTAGTCCCTTGAAT+CGG 0.485260 5.4:+66098979 None:intergenic
AGATTTGTTGCTGACAATCC+AGG 0.489827 5.4:-66098739 MS.gene94205:intron
AAACTCGTGCTCATGAACTT+TGG 0.490870 5.4:+66101746 None:intergenic
ACCTTGTTGATCCACCTATG+AGG 0.490977 5.4:-66098801 MS.gene94205:CDS
TTATTAATTAGGGGAATGGT+GGG 0.493359 5.4:-66100191 MS.gene94205:intron
ACAGCAACAGTGTTCCTCAT+AGG 0.495749 5.4:+66098787 None:intergenic
TTGGATGTTCACATTGGATG+GGG 0.496559 5.4:-66098180 MS.gene94205:CDS
TTGGATTATGCATTGTCACT+TGG 0.496947 5.4:-66098199 MS.gene94205:CDS
AACCATAAACTAACAGTAGT+AGG 0.497147 5.4:-66099585 MS.gene94205:CDS
GGGGTTAGACAAATCAGAAC+AGG 0.499855 5.4:-66100751 MS.gene94205:CDS
GATAAGCATCTCCTTCCTTA+GGG 0.506054 5.4:+66100572 None:intergenic
TGATCATTACCTAGAAGAAT+GGG 0.508193 5.4:+66100523 None:intergenic
ATGAGAACATTGGTTGTGAA+TGG 0.509668 5.4:+66099540 None:intergenic
TCATTGTAAGCAGGCAATGA+AGG 0.510001 5.4:+66099327 None:intergenic
TCACGCTTTGGCTTAGTGAA+AGG 0.510131 5.4:+66100550 None:intergenic
CTCCAAGTTTATGAGCCTGC+AGG 0.510477 5.4:+66099088 None:intergenic
ATCATCACCTTGTCCTGCAA+AGG 0.514426 5.4:-66099376 MS.gene94205:CDS
GTGATATTATTACCAATGAA+TGG 0.515384 5.4:+66101475 None:intergenic
AGATGAATAGGGTGATTCTC+AGG 0.517564 5.4:+66098898 None:intergenic
ATCAGAACAGGATGGGCTGA+TGG 0.518148 5.4:-66100739 MS.gene94205:CDS
TTTGCAATTGTGAAGAAAAG+AGG 0.519484 5.4:+66099606 None:intergenic
ACTCAATGTCCTATTCGTCC+TGG 0.520865 5.4:-66100703 MS.gene94205:CDS
GATTATCCTAACACAAAGGC+AGG 0.522293 5.4:+66098088 None:intergenic
GCTCATAAACTTGGAGTACA+AGG 0.524236 5.4:-66099081 MS.gene94205:CDS
CAGCATTGATTACTCTTAGG+AGG 0.526575 5.4:+66099640 None:intergenic
GAAGTGAACCGTGTCCCGTT+CGG 0.526770 5.4:+66099156 None:intergenic
TTGATGGTGGTTGGTCACCT+TGG 0.528026 5.4:+66099484 None:intergenic
TTTACCATTAATGGACAAGA+AGG 0.529526 5.4:-66100661 MS.gene94205:CDS
TGATTTGCATTAAAATTCGA+CGG 0.538672 5.4:+66099189 None:intergenic
TGATTTCTATATTGTTGCTG+AGG 0.540678 5.4:-66098866 MS.gene94205:CDS
CTTTACAAGTGCTCTACCAA+AGG 0.542011 5.4:-66100073 MS.gene94205:intron
AGAACATCAGTGGTTTGGCC+AGG 0.543220 5.4:+66099510 None:intergenic
TTTAATGCAAATCAATGTCA+AGG 0.544640 5.4:-66099180 MS.gene94205:CDS
ATCACCTTGTCCTGCAAAGG+GGG 0.545026 5.4:-66099373 MS.gene94205:CDS
CTATAGGTGTAACTTTCTCC+AGG 0.545148 5.4:+66100685 None:intergenic
TAAGTTGAGATTTGGATCAA+GGG 0.547755 5.4:-66098956 MS.gene94205:CDS
CCATCAAGATACTACATCGC+CGG 0.549313 5.4:-66099468 MS.gene94205:CDS
GATTTCTATATTGTTGCTGA+GGG 0.549381 5.4:-66098865 MS.gene94205:CDS
TGGAGCTGCTCCTGTTTGTG+TGG 0.553190 5.4:+66100138 None:intergenic
CATTGTGGCAACCGATTCAA+GGG 0.556129 5.4:-66098990 MS.gene94205:CDS
ACCGGTGTAATCAAATTGCA+CGG 0.556156 5.4:+66099026 None:intergenic
AGCTCTAATCATTCACCCTA+AGG 0.560869 5.4:-66100587 MS.gene94205:CDS
CATCAAGATACTACATCGCC+GGG 0.561153 5.4:-66099467 MS.gene94205:CDS
ACCACTGATGTTCTCATCCA+AGG 0.561174 5.4:-66099501 MS.gene94205:CDS
TTAATTTCCAAAGTTGGACC+AGG 0.564251 5.4:+66101551 None:intergenic
GACAAGAAGGTACACTTTGG+TGG 0.574088 5.4:-66100648 MS.gene94205:CDS
GTGTTTGTGTCATTGTAAGC+AGG 0.578541 5.4:+66099318 None:intergenic
TACACTATTAATGGTCAACC+TGG 0.583665 5.4:-66100100 MS.gene94205:CDS
GTCAAGGACCGAACGGGACA+CGG 0.584236 5.4:-66099164 MS.gene94205:CDS
CTAATCATTCACCCTAAGGA+AGG 0.586825 5.4:-66100583 MS.gene94205:CDS
TGTAGTATCTTGATGGTGGT+TGG 0.588008 5.4:+66099475 None:intergenic
GGTTGTGAATGGTTTGACAT+AGG 0.595120 5.4:+66099551 None:intergenic
ATGGACAAGAAGGTACACTT+TGG 0.605647 5.4:-66100651 MS.gene94205:CDS
TTTGTATTCAAGAATGGCAG+TGG 0.605780 5.4:+66099398 None:intergenic
ATTGTTGAGTCCTAATCCAA+TGG 0.608848 5.4:+66099215 None:intergenic
TAGACAAATCAGAACAGGAT+GGG 0.611575 5.4:-66100746 MS.gene94205:CDS
AATCAATGTCAAGGACCGAA+CGG 0.611682 5.4:-66099171 MS.gene94205:CDS
TACAAGCAACTCCAGTGAAG+AGG 0.611814 5.4:-66101616 MS.gene94205:CDS
GAGCACTTGTAAAGATCACC+AGG 0.619435 5.4:+66100082 None:intergenic
GCAACAGTGTTCCTCATAGG+TGG 0.621280 5.4:+66098790 None:intergenic
TGTCACTTGGATGTTCACAT+TGG 0.623581 5.4:-66098186 MS.gene94205:CDS
GTGTAATCAAATTGCACGGG+AGG 0.625603 5.4:+66099030 None:intergenic
CCGGTGTAATCAAATTGCAC+GGG 0.626025 5.4:+66099027 None:intergenic
ATTACACCGGTAATGTGAGT+CGG 0.633330 5.4:-66099014 MS.gene94205:CDS
GTCCATAGAAGCTCCTCCTG+AGG 0.638740 5.4:-66098112 MS.gene94205:CDS
ACCTTGGATGAGAACATCAG+TGG 0.638752 5.4:+66099500 None:intergenic
AATGTTCTCATGTTAGGACC+TGG 0.640008 5.4:-66099528 MS.gene94205:CDS
CACAAAGGCAGGTCCTCAGG+AGG 0.658358 5.4:+66098099 None:intergenic
TCACCTTGTCCTGCAAAGGG+GGG 0.659071 5.4:-66099372 MS.gene94205:CDS
TTTGGATTATCCTAACACAA+AGG 0.661322 5.4:+66098084 None:intergenic
GTAATGTGAGTCGGTCATTG+TGG 0.663037 5.4:-66099005 MS.gene94205:CDS
TCCTCATAGGTGGATCAACA+AGG 0.663984 5.4:+66098800 None:intergenic
CCCGTGCAATTTGATTACAC+CGG 0.670640 5.4:-66099027 MS.gene94205:CDS
CATCACCTTGTCCTGCAAAG+GGG 0.673225 5.4:-66099374 MS.gene94205:CDS
TTAGACAAATCAGAACAGGA+TGG 0.677485 5.4:-66100747 MS.gene94205:CDS
AGGTCCTAACATGAGAACAT+TGG 0.679248 5.4:+66099530 None:intergenic
GATTAGAGCTCCATAAACTG+TGG 0.691733 5.4:+66100600 None:intergenic
GCGATGTAGTATCTTGATGG+TGG 0.696908 5.4:+66099471 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTTTAAAAAAAAAT+AGG + chr5.4:66098519-66098538 None:intergenic 0.0%
!! AAACTTTATCATAAAAATTA+AGG + chr5.4:66101470-66101489 None:intergenic 10.0%
!! ACTATAAAAATACTATTATA+TGG + chr5.4:66098953-66098972 None:intergenic 10.0%
!! ATGATGATTATTATTAATTA+GGG - chr5.4:66099701-66099720 MS.gene94205:intron 10.0%
!! ATTAACAACTAAAAATAAAA+AGG + chr5.4:66098211-66098230 None:intergenic 10.0%
!! CAATTAATTAATTATGTATA+TGG + chr5.4:66100056-66100075 None:intergenic 10.0%
!! TATGATGATTATTATTAATT+AGG - chr5.4:66099700-66099719 MS.gene94205:intron 10.0%
!! TTAACAACTAAAAATAAAAA+GGG + chr5.4:66098210-66098229 None:intergenic 10.0%
!!! ATAATAGTATTTTTATAGTA+GGG - chr5.4:66098954-66098973 MS.gene94205:CDS 10.0%
!!! ATTTTGAATAAAATTTATGT+AGG + chr5.4:66101293-66101312 None:intergenic 10.0%
!!! GAAAAAATTGTAAATTTTAT+AGG - chr5.4:66101426-66101445 MS.gene94205:intron 10.0%
!!! TATAATAGTATTTTTATAGT+AGG - chr5.4:66098953-66098972 MS.gene94205:CDS 10.0%
!!! TTTTTTCAATTTGTTAATTA+AGG - chr5.4:66099566-66099585 MS.gene94205:CDS 10.0%
!! AAAACTAAAAAGTAACTATA+TGG + chr5.4:66098848-66098867 None:intergenic 15.0%
!! AGATAAGAATTAAAATAAGT+TGG + chr5.4:66099524-66099543 None:intergenic 15.0%
!! AGTGTAATAACAAATTTAAT+TGG - chr5.4:66098699-66098718 MS.gene94205:intron 15.0%
!! GGAGATTAATTATAATTAAT+TGG - chr5.4:66098241-66098260 MS.gene94205:intron 15.0%
!! TAAAAATACTATTATATGGA+AGG + chr5.4:66098949-66098968 None:intergenic 15.0%
!! TCAAAAATCAAAAACAAAAA+AGG + chr5.4:66098902-66098921 None:intergenic 15.0%
!! TGATGATTATTATTAATTAG+GGG - chr5.4:66099702-66099721 MS.gene94205:intron 15.0%
!! TGCAAATAATCAATAATTTA+TGG - chr5.4:66099095-66099114 MS.gene94205:CDS 15.0%
!! TTATATTCCTATTAGTATAT+AGG - chr5.4:66098625-66098644 MS.gene94205:intron 15.0%
!!! ACCACAATTTAAAATTTTAA+TGG - chr5.4:66099029-66099048 MS.gene94205:CDS 15.0%
!!! ACCATTAAAATTTTAAATTG+TGG + chr5.4:66099033-66099052 None:intergenic 15.0%
!!! TAAGCAAAAATCAATATTTT+AGG + chr5.4:66100085-66100104 None:intergenic 15.0%
!!! TTTTCATAAAAAAAAGTAAC+CGG - chr5.4:66100018-66100037 MS.gene94205:intron 15.0%
!! AATCTTTGTCTAAAAAGAAA+AGG + chr5.4:66099643-66099662 None:intergenic 20.0%
!! AATGATCATATGTCAAATAA+TGG + chr5.4:66100195-66100214 None:intergenic 20.0%
!! AATTATAATTAATCTCCCAA+AGG + chr5.4:66098238-66098257 None:intergenic 20.0%
!! ACATAAAATAATCAAACCTT+TGG + chr5.4:66099848-66099867 None:intergenic 20.0%
!! ATGCATGCTATAATTTATAA+AGG - chr5.4:66099062-66099081 MS.gene94205:CDS 20.0%
!! ATGTTTGATCTTATGTAATT+TGG - chr5.4:66101659-66101678 MS.gene94205:intron 20.0%
!! ATTATTATTAATTAGGGGAA+TGG - chr5.4:66099707-66099726 MS.gene94205:intron 20.0%
!! ATTATTTGACATATGATCAT+TGG - chr5.4:66100194-66100213 MS.gene94205:intron 20.0%
!! GTCTCAATTAAATCAAAATA+AGG - chr5.4:66101368-66101387 MS.gene94205:intron 20.0%
!! TAGTTGTTAATTTAACCTTT+GGG - chr5.4:66098220-66098239 MS.gene94205:intron 20.0%
!! TTAGTTGTTAATTTAACCTT+TGG - chr5.4:66098219-66098238 MS.gene94205:intron 20.0%
!! TTGAATAAAATTTATGTAGG+AGG + chr5.4:66101290-66101309 None:intergenic 20.0%
!!! AAAGTTAATAAGTTGAGATT+TGG - chr5.4:66100938-66100957 MS.gene94205:intron 20.0%
!!! AATTAATAATGGAGATACTT+TGG - chr5.4:66098368-66098387 MS.gene94205:intron 20.0%
!!! ATTAGGGTTAATTATGTTTT+CGG - chr5.4:66101229-66101248 MS.gene94205:intron 20.0%
!!! TACTTTTCCTATATACTAAT+AGG + chr5.4:66098635-66098654 None:intergenic 20.0%
!!! TTATTGCATTTTGAATCTTT+AGG - chr5.4:66099125-66099144 MS.gene94205:CDS 20.0%
!!! TTTTTGCTTATAATAGTGAT+CGG - chr5.4:66100095-66100114 MS.gene94205:CDS 20.0%
! AAAGTTTCACAAACATGATA+TGG - chr5.4:66101483-66101502 MS.gene94205:intron 25.0%
! AAGAAAAATTGCTAAGAAGA+AGG + chr5.4:66098114-66098133 None:intergenic 25.0%
! AAGAATTCATAAGTGATTGA+AGG + chr5.4:66100142-66100161 None:intergenic 25.0%
! AAGGTGTAAGAAAACAAAAA+AGG + chr5.4:66098930-66098949 None:intergenic 25.0%
! AATGGAAAAATGTTTAGTCA+TGG + chr5.4:66100177-66100196 None:intergenic 25.0%
! ACCTATAGATTTACCATTAA+TGG - chr5.4:66099232-66099251 MS.gene94205:CDS 25.0%
! AGATCTTCATCAATTTCATT+AGG + chr5.4:66100659-66100678 None:intergenic 25.0%
! ATAAATAGCAATGCTCAAAA+AGG - chr5.4:66098764-66098783 MS.gene94205:CDS 25.0%
! CCATTATTAATTTCCAAAGT+TGG + chr5.4:66098360-66098379 None:intergenic 25.0%
! GCATGTTTATTAATGTGAAA+AGG - chr5.4:66098263-66098282 MS.gene94205:intron 25.0%
! GGAAAAGTAAATGATAGATA+CGG - chr5.4:66098646-66098665 MS.gene94205:intron 25.0%
! GTGATATTATTACCAATGAA+TGG + chr5.4:66098430-66098449 None:intergenic 25.0%
! TAAATAGCAATGCTCAAAAA+GGG - chr5.4:66098765-66098784 MS.gene94205:CDS 25.0%
! TCCATTAATGGTAAATCTAT+AGG + chr5.4:66099236-66099255 None:intergenic 25.0%
! TTTAATGCAAATCAATGTCA+AGG - chr5.4:66100722-66100741 MS.gene94205:CDS 25.0%
!! CCAACTTTGGAAATTAATAA+TGG - chr5.4:66098357-66098376 MS.gene94205:intron 25.0%
!! TATATGGTCTTTTTAAATGC+CGG + chr5.4:66100040-66100059 None:intergenic 25.0%
!! TGATGATTTGTATTCAAGAA+TGG + chr5.4:66100513-66100532 None:intergenic 25.0%
!! TGATTTGCATTAAAATTCGA+CGG + chr5.4:66100716-66100735 None:intergenic 25.0%
!!! AATTTTGAAGCATCTTTCTT+AGG + chr5.4:66101079-66101098 None:intergenic 25.0%
!!! ATTTTGAAGCATCTTTCTTA+GGG + chr5.4:66101078-66101097 None:intergenic 25.0%
!!! GTAAAAGCTTTAGAAGTTTA+AGG - chr5.4:66100621-66100640 MS.gene94205:CDS 25.0%
AAATGAGTTAAGACAAGTCA+GGG - chr5.4:66098816-66098835 MS.gene94205:CDS 30.0%
AACCATAAACTAACAGTAGT+AGG - chr5.4:66100317-66100336 MS.gene94205:intron 30.0%
ATCAAAATAAGGACTGAAAG+TGG - chr5.4:66101379-66101398 MS.gene94205:intron 30.0%
ATGATCATTACCTAGAAGAA+TGG + chr5.4:66099383-66099402 None:intergenic 30.0%
ATTATTAATTAGGGGAATGG+TGG - chr5.4:66099710-66099729 MS.gene94205:intron 30.0%
CATATCCATGAAGATGAATA+GGG + chr5.4:66101018-66101037 None:intergenic 30.0%
GACAAAGATTATGTTCATGA+TGG - chr5.4:66099653-66099672 MS.gene94205:CDS 30.0%
GATTTCTATATTGTTGCTGA+GGG - chr5.4:66101037-66101056 MS.gene94205:intron 30.0%
GTGTATGCATCAGATATATT+TGG + chr5.4:66099787-66099806 None:intergenic 30.0%
TAAATGAGTTAAGACAAGTC+AGG - chr5.4:66098815-66098834 MS.gene94205:CDS 30.0%
TCATATCCATGAAGATGAAT+AGG + chr5.4:66101019-66101038 None:intergenic 30.0%
TCTGATGCATACACTATTAA+TGG - chr5.4:66099793-66099812 MS.gene94205:intron 30.0%
TGATCATTACCTAGAAGAAT+GGG + chr5.4:66099382-66099401 None:intergenic 30.0%
TGATTTCTATATTGTTGCTG+AGG - chr5.4:66101036-66101055 MS.gene94205:intron 30.0%
TTAACTTTAGTCCCTTGAAT+CGG + chr5.4:66100926-66100945 None:intergenic 30.0%
TTATTAATTAGGGGAATGGT+GGG - chr5.4:66099711-66099730 MS.gene94205:intron 30.0%
TTTGCAATTGTGAAGAAAAG+AGG + chr5.4:66100299-66100318 None:intergenic 30.0%
TTTGGTTGTTTGATGTATGT+AGG - chr5.4:66101677-66101696 MS.gene94205:intron 30.0%
! ACAATTGTTCCAATTGATTC+AGG - chr5.4:66100230-66100249 MS.gene94205:intron 30.0%
! ATAAGTTGAGATTTGGATCA+AGG - chr5.4:66100945-66100964 MS.gene94205:intron 30.0%
! ATTTTGAATCTTTAGGCATG+GGG - chr5.4:66099132-66099151 MS.gene94205:CDS 30.0%
! CATTTTGAATCTTTAGGCAT+GGG - chr5.4:66099131-66099150 MS.gene94205:CDS 30.0%
! CTTAGCAATTTTTCTTCTCT+TGG - chr5.4:66098118-66098137 MS.gene94205:CDS 30.0%
! CTTTTCTTTACCATTGGATT+AGG - chr5.4:66100677-66100696 MS.gene94205:CDS 30.0%
! GAAGATCTTTTCTTTACCAT+TGG - chr5.4:66100671-66100690 MS.gene94205:CDS 30.0%
! TAAGTTGAGATTTGGATCAA+GGG - chr5.4:66100946-66100965 MS.gene94205:intron 30.0%
! TTTACCATTAATGGACAAGA+AGG - chr5.4:66099241-66099260 MS.gene94205:CDS 30.0%
! TTTGTTTCTCCTGAATCAAT+TGG + chr5.4:66100242-66100261 None:intergenic 30.0%
AAAAGGAATGTCACTCTACA+TGG + chr5.4:66098885-66098904 None:intergenic 35.0%
AATCACCCTATTCATCTTCA+TGG - chr5.4:66101010-66101029 MS.gene94205:intron 35.0%
ACAACCAATGTTCTCATGTT+AGG - chr5.4:66100368-66100387 MS.gene94205:intron 35.0%
ATTGTTGAGTCCTAATCCAA+TGG + chr5.4:66100690-66100709 None:intergenic 35.0%
CTTGTCTCACAACATCAATT+GGG + chr5.4:66099744-66099763 None:intergenic 35.0%
GAAATTGAGCTAATGTTACC+TGG + chr5.4:66101184-66101203 None:intergenic 35.0%
GAGACTAAGTACATGACTAA+AGG + chr5.4:66101353-66101372 None:intergenic 35.0%
GCAGTCAAATGAAATTGATG+TGG + chr5.4:66101554-66101573 None:intergenic 35.0%
GTTACAATGTCACCATTCAT+TGG - chr5.4:66098415-66098434 MS.gene94205:intron 35.0%
GTTTGATGTATGTAGGTGTT+TGG - chr5.4:66101684-66101703 MS.gene94205:intron 35.0%
TACACTATTAATGGTCAACC+TGG - chr5.4:66099802-66099821 MS.gene94205:intron 35.0%
TAGACAAATCAGAACAGGAT+GGG - chr5.4:66099156-66099175 MS.gene94205:CDS 35.0%
TATATTGTTGCTGAGGGATT+TGG - chr5.4:66101043-66101062 MS.gene94205:intron 35.0%
TCTTAGTACAAGTGTGAAGA+AGG + chr5.4:66098583-66098602 None:intergenic 35.0%
TGCAGGATTGAAAAATTGCT+AGG + chr5.4:66100800-66100819 None:intergenic 35.0%
TGTACCTTCTTGTCCATTAA+TGG + chr5.4:66099248-66099267 None:intergenic 35.0%
TTAATTTCCAAAGTTGGACC+AGG + chr5.4:66098354-66098373 None:intergenic 35.0%
TTAGACAAATCAGAACAGGA+TGG - chr5.4:66099155-66099174 MS.gene94205:CDS 35.0%
TTGCTTATAATAGTGATCGG+AGG - chr5.4:66100098-66100117 MS.gene94205:CDS 35.0%
TTGGATTATGCATTGTCACT+TGG - chr5.4:66101703-66101722 MS.gene94205:intron 35.0%
TTTGTATTCAAGAATGGCAG+TGG + chr5.4:66100507-66100526 None:intergenic 35.0%
! ATGAGAACATTGGTTGTGAA+TGG + chr5.4:66100365-66100384 None:intergenic 35.0%
! CACCTACTACTGTTAGTTTA+TGG + chr5.4:66100322-66100341 None:intergenic 35.0%
! GATTATGTTCATGATGGAGA+TGG - chr5.4:66099659-66099678 MS.gene94205:CDS 35.0%
! GCATTTTGAATCTTTAGGCA+TGG - chr5.4:66099130-66099149 MS.gene94205:CDS 35.0%
! TCATGATGGAGATGGTTTAA+CGG - chr5.4:66099667-66099686 MS.gene94205:CDS 35.0%
!! AAAAAGTGTTTGCTTGCTTG+TGG + chr5.4:66098556-66098575 None:intergenic 35.0%
!! AAAAGTGTTTGCTTGCTTGT+GGG + chr5.4:66098555-66098574 None:intergenic 35.0%
!! AGTACAAGGTGTTTTTACGA+CGG - chr5.4:66100835-66100854 MS.gene94205:intron 35.0%
!! TAGCAATTTTTCAATCCTGC+AGG - chr5.4:66100799-66100818 MS.gene94205:intron 35.0%
AAACTCGTGCTCATGAACTT+TGG + chr5.4:66098159-66098178 None:intergenic 40.0%
AATCAATGTCAAGGACCGAA+CGG - chr5.4:66100731-66100750 MS.gene94205:CDS 40.0%
AATGTTCTCATGTTAGGACC+TGG - chr5.4:66100374-66100393 MS.gene94205:intron 40.0%
AATTGATGTGGCCTCAGTTA+TGG + chr5.4:66101542-66101561 None:intergenic 40.0%
ACAATTTCCTGGTCCAACTT+TGG - chr5.4:66098344-66098363 MS.gene94205:intron 40.0%
ACCGGTGTAATCAAATTGCA+CGG + chr5.4:66100879-66100898 None:intergenic 40.0%
ACTGTGAATGGACAATTTCC+TGG - chr5.4:66098333-66098352 MS.gene94205:intron 40.0%
ACTGTTGCTGTACCTGTTAA+TGG - chr5.4:66101127-66101146 MS.gene94205:intron 40.0%
ACTTGGATGTTCACATTGGA+TGG - chr5.4:66101720-66101739 MS.gene94205:intron 40.0%
AGATGAATAGGGTGATTCTC+AGG + chr5.4:66101007-66101026 None:intergenic 40.0%
AGGTCCTAACATGAGAACAT+TGG + chr5.4:66100375-66100394 None:intergenic 40.0%
ATAACTGCCCAACCATTAAC+AGG + chr5.4:66101142-66101161 None:intergenic 40.0%
ATTACACCGGTAATGTGAGT+CGG - chr5.4:66100888-66100907 MS.gene94205:intron 40.0%
CAAATGAGCATCACTGTGAA+TGG - chr5.4:66098321-66098340 MS.gene94205:intron 40.0%
CAGGACCAAGTATCTATATG+CGG - chr5.4:66101583-66101602 MS.gene94205:CDS 40.0%
CATATGATCATTGGCAATGC+AGG - chr5.4:66100203-66100222 MS.gene94205:intron 40.0%
CTAATCATTCACCCTAAGGA+AGG - chr5.4:66099319-66099338 MS.gene94205:CDS 40.0%
CTATAGGTGTAACTTTCTCC+AGG + chr5.4:66099220-66099239 None:intergenic 40.0%
CTTGGATGTTCACATTGGAT+GGG - chr5.4:66101721-66101740 MS.gene94205:intron 40.0%
CTTTACAAGTGCTCTACCAA+AGG - chr5.4:66099829-66099848 MS.gene94205:intron 40.0%
GATAAGCATCTCCTTCCTTA+GGG + chr5.4:66099333-66099352 None:intergenic 40.0%
GATTAGAGCTCCATAAACTG+TGG + chr5.4:66099305-66099324 None:intergenic 40.0%
GCGGTTGCGATATAAAACTT+TGG - chr5.4:66101602-66101621 MS.gene94205:CDS 40.0%
GCTCATAAACTTGGAGTACA+AGG - chr5.4:66100821-66100840 MS.gene94205:intron 40.0%
GCTTGTCTCACAACATCAAT+TGG + chr5.4:66099745-66099764 None:intergenic 40.0%
GGACATTGAGTCACAAATTC+TGG + chr5.4:66099190-66099209 None:intergenic 40.0%
GGTTGTGAATGGTTTGACAT+AGG + chr5.4:66100354-66100373 None:intergenic 40.0%
TAACTTTCTCCAGGACGAAT+AGG + chr5.4:66099211-66099230 None:intergenic 40.0%
TCATTGTAAGCAGGCAATGA+AGG + chr5.4:66100578-66100597 None:intergenic 40.0%
TGCTGTACCTGTTAATGGTT+GGG - chr5.4:66101132-66101151 MS.gene94205:intron 40.0%
TTGCTGTACCTGTTAATGGT+TGG - chr5.4:66101131-66101150 MS.gene94205:intron 40.0%
TTGGATGTTCACATTGGATG+GGG - chr5.4:66101722-66101741 MS.gene94205:intron 40.0%
! AGCTCTAATCATTCACCCTA+AGG - chr5.4:66099315-66099334 MS.gene94205:CDS 40.0%
! ATGGACAAGAAGGTACACTT+TGG - chr5.4:66099251-66099270 MS.gene94205:CDS 40.0%
! GTGTTTGTGTCATTGTAAGC+AGG + chr5.4:66100587-66100606 None:intergenic 40.0%
! TGTCACTTGGATGTTCACAT+TGG - chr5.4:66101716-66101735 MS.gene94205:intron 40.0%
! TGTTTCTGTGACCATAACTG+AGG - chr5.4:66101528-66101547 MS.gene94205:CDS 40.0%
! TTTTGGTAGAGGATGGAGAA+GGG - chr5.4:66101759-66101778 MS.gene94205:CDS 40.0%
!! AGATTTGTTGCTGACAATCC+AGG - chr5.4:66101163-66101182 MS.gene94205:intron 40.0%
!! CAGCATTGATTACTCTTAGG+AGG + chr5.4:66100265-66100284 None:intergenic 40.0%
!! GTGCAGCATTGATTACTCTT+AGG + chr5.4:66100268-66100287 None:intergenic 40.0%
!! TGTAGTATCTTGATGGTGGT+TGG + chr5.4:66100430-66100449 None:intergenic 40.0%
!! TTTTTGGTAGAGGATGGAGA+AGG - chr5.4:66101758-66101777 MS.gene94205:CDS 40.0%
!!! ACTGTGTTTTTGGTAGAGGA+TGG - chr5.4:66101752-66101771 MS.gene94205:CDS 40.0%
ACAGCAACAGTGTTCCTCAT+AGG + chr5.4:66101118-66101137 None:intergenic 45.0%
ACCACTGATGTTCTCATCCA+AGG - chr5.4:66100401-66100420 MS.gene94205:intron 45.0%
ACCTTGGATGAGAACATCAG+TGG + chr5.4:66100405-66100424 None:intergenic 45.0%
ACCTTGTTGATCCACCTATG+AGG - chr5.4:66101101-66101120 MS.gene94205:intron 45.0%
ACTCAATGTCCTATTCGTCC+TGG - chr5.4:66099199-66099218 MS.gene94205:CDS 45.0%
AGAATGGGTGTTTCACGCTT+TGG + chr5.4:66099367-66099386 None:intergenic 45.0%
ATCAATGTCAAGGACCGAAC+GGG - chr5.4:66100732-66100751 MS.gene94205:CDS 45.0%
ATCATCACCTTGTCCTGCAA+AGG - chr5.4:66100526-66100545 MS.gene94205:intron 45.0%
ATCCTGCAGGCTCATAAACT+TGG - chr5.4:66100812-66100831 MS.gene94205:intron 45.0%
ATTTGACTGCGATTCTCTGC+AGG - chr5.4:66101564-66101583 MS.gene94205:CDS 45.0%
CATCAAGATACTACATCGCC+GGG - chr5.4:66100435-66100454 MS.gene94205:intron 45.0%
CCATCAAGATACTACATCGC+CGG - chr5.4:66100434-66100453 MS.gene94205:intron 45.0%
CCCGTGCAATTTGATTACAC+CGG - chr5.4:66100875-66100894 MS.gene94205:intron 45.0%
CCGGTGTAATCAAATTGCAC+GGG + chr5.4:66100878-66100897 None:intergenic 45.0%
CGCAACCGCATATAGATACT+TGG + chr5.4:66101591-66101610 None:intergenic 45.0%
CGTGAAACACCCATTCTTCT+AGG - chr5.4:66099370-66099389 MS.gene94205:CDS 45.0%
GAGATTTGGATCAAGGGTTC+AGG - chr5.4:66100952-66100971 MS.gene94205:intron 45.0%
GAGCACTTGTAAAGATCACC+AGG + chr5.4:66099823-66099842 None:intergenic 45.0%
GCGATGTAGTATCTTGATGG+TGG + chr5.4:66100434-66100453 None:intergenic 45.0%
GGATAAGCATCTCCTTCCTT+AGG + chr5.4:66099334-66099353 None:intergenic 45.0%
GGATGAGAACATCAGTGGTT+TGG + chr5.4:66100400-66100419 None:intergenic 45.0%
GGGGTTAGACAAATCAGAAC+AGG - chr5.4:66099151-66099170 MS.gene94205:CDS 45.0%
GTAATGTGAGTCGGTCATTG+TGG - chr5.4:66100897-66100916 MS.gene94205:intron 45.0%
GTGTAATCAAATTGCACGGG+AGG + chr5.4:66100875-66100894 None:intergenic 45.0%
TACAAGCAACTCCAGTGAAG+AGG - chr5.4:66098286-66098305 MS.gene94205:intron 45.0%
TCATCACCTTGTCCTGCAAA+GGG - chr5.4:66100527-66100546 MS.gene94205:intron 45.0%
TCCTCATAGGTGGATCAACA+AGG + chr5.4:66101105-66101124 None:intergenic 45.0%
TGGCTTAGAGCCACAGTTTA+TGG - chr5.4:66099292-66099311 MS.gene94205:CDS 45.0%
TGTTCACATTGGATGGGGTT+TGG - chr5.4:66101727-66101746 MS.gene94205:intron 45.0%
! AAATTGCACGGGAGGTTTTG+TGG + chr5.4:66100867-66100886 None:intergenic 45.0%
! CAATGACCGACTCACATTAC+CGG + chr5.4:66100897-66100916 None:intergenic 45.0%
! CATTGTGGCAACCGATTCAA+GGG - chr5.4:66100912-66100931 MS.gene94205:intron 45.0%
! GACAAGAAGGTACACTTTGG+TGG - chr5.4:66099254-66099273 MS.gene94205:CDS 45.0%
! GGGTTTGGCTACTGTGTTTT+TGG - chr5.4:66101742-66101761 MS.gene94205:intron 45.0%
! TCACGCTTTGGCTTAGTGAA+AGG + chr5.4:66099355-66099374 None:intergenic 45.0%
! TCATTGTGGCAACCGATTCA+AGG - chr5.4:66100911-66100930 MS.gene94205:intron 45.0%
!! AATTGCACGGGAGGTTTTGT+GGG + chr5.4:66100866-66100885 None:intergenic 45.0%
!!! GGCTACTGTGTTTTTGGTAG+AGG - chr5.4:66101748-66101767 MS.gene94205:CDS 45.0%
!! ATTGTATAAAAAATATAATT+AGG - chr5.4:66101212-66101231 MS.gene94205:intron 5.0%
!! TGTAAAAATTAATAAAAATA+GGG + chr5.4:66101320-66101339 None:intergenic 5.0%
!! TTGTAAAAATTAATAAAAAT+AGG + chr5.4:66101321-66101340 None:intergenic 5.0%
!! TTGTATAAAAAATATAATTA+GGG - chr5.4:66101213-66101232 MS.gene94205:intron 5.0%
!!! AAAAAATTGTAAATTTTATA+GGG - chr5.4:66101427-66101446 MS.gene94205:intron 5.0%
!!! TTTTATTAATTTTTACAAAA+TGG - chr5.4:66101323-66101342 MS.gene94205:intron 5.0%
AGAGGATGGAGAAGGGCTAT+TGG - chr5.4:66101766-66101785 MS.gene94205:CDS 50.0%
ATATTTGCACCCCCCTTTGC+AGG + chr5.4:66100542-66100561 None:intergenic 50.0%
ATCACCTTGTCCTGCAAAGG+GGG - chr5.4:66100529-66100548 MS.gene94205:intron 50.0%
CATCACCTTGTCCTGCAAAG+GGG - chr5.4:66100528-66100547 MS.gene94205:intron 50.0%
CCGGCGATGTAGTATCTTGA+TGG + chr5.4:66100437-66100456 None:intergenic 50.0%
CTCCAAGTTTATGAGCCTGC+AGG + chr5.4:66100817-66100836 None:intergenic 50.0%
GCAACAGTGTTCCTCATAGG+TGG + chr5.4:66101115-66101134 None:intergenic 50.0%
GGTGGCATGCTCATAGTTCA+TGG - chr5.4:66099272-66099291 MS.gene94205:CDS 50.0%
GTGAGACAAGCCACACAAAC+AGG - chr5.4:66099754-66099773 MS.gene94205:intron 50.0%
TAACACAAAGGCAGGTCCTC+AGG + chr5.4:66101809-66101828 None:intergenic 50.0%
! AGAACATCAGTGGTTTGGCC+AGG + chr5.4:66100395-66100414 None:intergenic 50.0%
! GCAGGCAATGAAGGCATGAT+TGG + chr5.4:66100569-66100588 None:intergenic 50.0%
!! ATCAGAACAGGATGGGCTGA+TGG - chr5.4:66099163-66099182 MS.gene94205:CDS 50.0%
!! TTGATGGTGGTTGGTCACCT+TGG + chr5.4:66100421-66100440 None:intergenic 50.0%
GAAGTGAACCGTGTCCCGTT+CGG + chr5.4:66100749-66100768 None:intergenic 55.0%
GTCCATAGAAGCTCCTCCTG+AGG - chr5.4:66101790-66101809 MS.gene94205:CDS 55.0%
GTCCTCAGGAGGAGCTTCTA+TGG + chr5.4:66101795-66101814 None:intergenic 55.0%
GTGTTGCACAGCCTCTTCAC+TGG + chr5.4:66098300-66098319 None:intergenic 55.0%
TCACCTTGTCCTGCAAAGGG+GGG - chr5.4:66100530-66100549 MS.gene94205:intron 55.0%
TGGAGCTGCTCCTGTTTGTG+TGG + chr5.4:66099767-66099786 None:intergenic 55.0%
!! ACGCAGCGTTTTGAGCCGAT+TGG + chr5.4:66100469-66100488 None:intergenic 55.0%
CACAAAGGCAGGTCCTCAGG+AGG + chr5.4:66101806-66101825 None:intergenic 60.0%
GTCAAGGACCGAACGGGACA+CGG - chr5.4:66100738-66100757 MS.gene94205:CDS 60.0%
AGCCGATTGGTAAGCGCGCC+CGG + chr5.4:66100456-66100475 None:intergenic 65.0%
GCACCCCCCTTTGCAGGACA+AGG + chr5.4:66100536-66100555 None:intergenic 65.0%
CGCCGGGCGCGCTTACCAAT+CGG - chr5.4:66100451-66100470 MS.gene94205:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 66098095 66101829 66098095 ID=MS.gene94205
chr5.4 mRNA 66098095 66101829 66098095 ID=MS.gene94205.t1;Parent=MS.gene94205
chr5.4 exon 66101743 66101829 66101743 ID=MS.gene94205.t1.exon1;Parent=MS.gene94205.t1
chr5.4 CDS 66101743 66101829 66101743 ID=cds.MS.gene94205.t1;Parent=MS.gene94205.t1
chr5.4 exon 66101488 66101639 66101488 ID=MS.gene94205.t1.exon2;Parent=MS.gene94205.t1
chr5.4 CDS 66101488 66101639 66101488 ID=cds.MS.gene94205.t1;Parent=MS.gene94205.t1
chr5.4 exon 66100533 66100777 66100533 ID=MS.gene94205.t1.exon3;Parent=MS.gene94205.t1
chr5.4 CDS 66100533 66100777 66100533 ID=cds.MS.gene94205.t1;Parent=MS.gene94205.t1
chr5.4 exon 66100074 66100202 66100074 ID=MS.gene94205.t1.exon4;Parent=MS.gene94205.t1
chr5.4 CDS 66100074 66100202 66100074 ID=cds.MS.gene94205.t1;Parent=MS.gene94205.t1
chr5.4 exon 66098740 66099699 66098740 ID=MS.gene94205.t1.exon5;Parent=MS.gene94205.t1
chr5.4 CDS 66098740 66099699 66098740 ID=cds.MS.gene94205.t1;Parent=MS.gene94205.t1
chr5.4 exon 66098095 66098225 66098095 ID=MS.gene94205.t1.exon6;Parent=MS.gene94205.t1
chr5.4 CDS 66098095 66098225 66098095 ID=cds.MS.gene94205.t1;Parent=MS.gene94205.t1
Gene Sequence

>MS.gene94205

ATGGAAGCTTTCAACACCTTCTTCTTAGCAATTTTTCTTCTCTTGGCTTCTTTAGCTAATGCCAAAGTTCATGAGCACGAGTTTGTCGTAAGTTTCTTTATTTTTTTTAAAGCCCTTTTTATTTTTAGTTGTTAATTTAACCTTTGGGAGATTAATTATAATTAATTGGCATGTTTATTAATGTGAAAAGGTACAAGCAACTCCAGTGAAGAGGCTGTGCAACACACAAATGAGCATCACTGTGAATGGACAATTTCCTGGTCCAACTTTGGAAATTAATAATGGAGATACTTTGGTTGTCAAAGTCATTAACAAAGCTCGTTACAATGTCACCATTCATTGGTAATAATATCACAAGCTAGCTAGAACTTTGTATATTAATTTTTATTTTATTTTGTTAACAAATTAATAGCTCACTTTGCCTATTTTTTTTTAAAAAAAATATTAGATCACTTTACCCACAAGCAAGCAAACACTTTTTATATCCTTCTTCACACTTGTACTAAGAATTGATTGCAAGCTCACATTAATTATATTCCTATTAGTATATAGGAAAAGTAAATGATAGATACGGTGACAAAATTTATCAAGAAAATTTCAAGACAGTGTAATAACAAATTTAATTGGTTGAGACATTTTCTTGATAAATTCTTGATAAAAACTCTCTAGATAAATAGCAATGCTCAAAAAGGGAAATATATTAAGCATCATGATTGTGAATAAATGAGTTAAGACAAGTCAGGGTATATACCATATAGTTACTTTTTAGTTTTCTAAATATGATCAACCATGTAGAGTGACATTCCTTTTTTGTTTTTGATTTTTGAAATTTCCTTTTTTGTTTTCTTACACCTTCCATATAATAGTATTTTTATAGTAGGGTATGCAATTTGAGCTGTATATTTTTGAAAAAAAAAAGTCAAGATTATTTACAACCACAATTTAAAATTTTAATGGTATGTTTTTCATGCATGCTATAATTTATAAAGGATCATATATATGCAAATAATCAATAATTTATGGCTTGATTTTATTGCATTTTGAATCTTTAGGCATGGGGTTAGACAAATCAGAACAGGATGGGCTGATGGACCAGAATTTGTGACTCAATGTCCTATTCGTCCTGGAGAAAGTTACACCTATAGATTTACCATTAATGGACAAGAAGGTACACTTTGGTGGCATGCTCATAGTTCATGGCTTAGAGCCACAGTTTATGGAGCTCTAATCATTCACCCTAAGGAAGGAGATGCTTATCCTTTCACTAAGCCAAAGCGTGAAACACCCATTCTTCTAGGTAATGATCATTGTATTTGACATCTAACTTTTTTTTTAAGAACTTTGCAAACGTGATTATTAGATTTTTATATCTGATGTAACTGATGTGTGTAACATCGACTTGTAAAATGAAGAATGTCATTCTTTACCAACTTATTTTAATTCTTATCTTTATTTAACGTGTGACTTAGATTTTTTTCAATTTGTTAATTAAGGATGTGATTGATATGAAATCATAAAGAAAATGAAATTATGAGATATGTTTGTCCTTTTCTTTTTAGACAAAGATTATGTTCATGATGGAGATGGTTTAACGGTTAAAATAATTATGATGATTATTATTAATTAGGGGAATGGTGGGATGCAAACCCAATTGATGTTGTGAGACAAGCCACACAAACAGGAGCAGCTCCAAATATATCTGATGCATACACTATTAATGGTCAACCTGGTGATCTTTACAAGTGCTCTACCAAAGGTTTGATTATTTTATGTTATTTTTTAATTTTAAGTAGATTTTTTAGTGAACTCATTATCAACATGTCACATATATGATTGATTAATTAATAAATTAAATAATTTTTTTATAAATTTACTATAGTCGAATATTTTATTTTAATTAGTTATAAGACAATCGTCAACAATTTTTCATAAAAAAAAGTAACCGGCATTTAAAAAGACCATATACATAATTAATTAATTGAATGAACCTAAAATATTGATTTTTGCTTATAATAGTGATCGGAGGATGTATGCAATAATAACACCTTCAATCACTTATGAATTCTTCATGATATATGACCATGACTAAACATTTTTCCATTATTTGACATATGATCATTGGCAATGCAGGCACAACAATTGTTCCAATTGATTCAGGAGAAACAAACCTCCTAAGAGTAATCAATGCTGCACTAAATCAACCTCTTTTCTTCACAATTGCAAACCATAAACTAACAGTAGTAGGTGCTGATGCTTCCTATGTCAAACCATTCACAACCAATGTTCTCATGTTAGGACCTGGCCAAACCACTGATGTTCTCATCCAAGGTGACCAACCACCATCAAGATACTACATCGCCGGGCGCGCTTACCAATCGGCTCAAAACGCTGCGTTCGATAACACAACAACCACTGCCATTCTTGAATACAAATCATCACCTTGTCCTGCAAAGGGGGGTGCAAATATCAGACCAATCATGCCTTCATTGCCTGCTTACAATGACACAAACACAGTTACATCATTTAGTAAAAGCTTTAGAAGTTTAAGGAATGTTGAAGTTCCTAATGAAATTGATGAAGATCTTTTCTTTACCATTGGATTAGGACTCAACAATTGTCCGTCGAATTTTAATGCAAATCAATGTCAAGGACCGAACGGGACACGGTTCACTTCTAGTATGAACAATGTGTCTTTTGTACTTCCTAGCAATTTTTCAATCCTGCAGGCTCATAAACTTGGAGTACAAGGTGTTTTTACGACGGATTTTCCCACAAAACCTCCCGTGCAATTTGATTACACCGGTAATGTGAGTCGGTCATTGTGGCAACCGATTCAAGGGACTAAAGTTAATAAGTTGAGATTTGGATCAAGGGTTCAGGTTGTTTTGCAAGATACTAGTATTGTTACTCCTGAGAATCACCCTATTCATCTTCATGGATATGATTTCTATATTGTTGCTGAGGGATTTGGAAATTATGACCCTAAGAAAGATGCTTCAAAATTTAACCTTGTTGATCCACCTATGAGGAACACTGTTGCTGTACCTGTTAATGGTTGGGCAGTTATCAGATTTGTTGCTGACAATCCAGGTAACATTAGCTCAATTTCAACTTTGCATTGTATAAAAAATATAATTAGGGTTAATTATGTTTTCGGTCTCTAAAAAATTAAGATTTTTTTTAAGTTTAATTCCTCCTACATAAATTTTATTCAAAATTAGTCCCTATTTTTATTAATTTTTACAAAATGGAGTACCTTTAGTCATGTACTTAGTCTCAATTAAATCAAAATAAGGACTGAAAGTGGACAATTTACGAAAAGATTAAACTCGAAAAAATTGTAAATTTTATAGGGATTAAAAATATATTTAACCTTAATTTTTATGATAAAGTTTCACAAACATGATATGGATCGTGACATCAATGTCTTTGATGTTTCTGTGACCATAACTGAGGCCACATCAATTTCATTTGACTGCGATTCTCTGCAGGACCAAGTATCTATATGCGGTTGCGATATAAAACTTTGGTATAATGAAGAGAAAAAACATAATCAATTATTCAATGTTTGATCTTATGTAATTTGGTTGTTTGATGTATGTAGGTGTTTGGATTATGCATTGTCACTTGGATGTTCACATTGGATGGGGTTTGGCTACTGTGTTTTTGGTAGAGGATGGAGAAGGGCTATTGGAGTCCATAGAAGCTCCTCCTGAGGACCTGCCTTTGTGTTAG
Protein sequence

>MS.gene94205.t1

MEAFNTFFLAIFLLLASLANAKVHEHEFVVQATPVKRLCNTQMSITVNGQFPGPTLEINNGDTLVVKVINKARYNVTIHWHGVRQIRTGWADGPEFVTQCPIRPGESYTYRFTINGQEGTLWWHAHSSWLRATVYGALIIHPKEGDAYPFTKPKRETPILLGEWWDANPIDVVRQATQTGAAPNISDAYTINGQPGDLYKCSTKGTTIVPIDSGETNLLRVINAALNQPLFFTIANHKLTVVGADASYVKPFTTNVLMLGPGQTTDVLIQGDQPPSRYYIAGRAYQSAQNAAFDNTTTTAILEYKSSPCPAKGGANIRPIMPSLPAYNDTNTVTSFSKSFRSLRNVEVPNEIDEDLFFTIGLGLNNCPSNFNANQCQGPNGTRFTSSMNNVSFVLPSNFSILQAHKLGVQGVFTTDFPTKPPVQFDYTGNVSRSLWQPIQGTKVNKLRFGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNYDPKKDASKFNLVDPPMRNTVAVPVNGWAVIRFVADNPGVWIMHCHLDVHIGWGLATVFLVEDGEGLLESIEAPPEDLPLC