AlfalfaGEDB | MS.gene94559

M. sativa cultivar XinJiangDaYe / MS.gene94559

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00515.31 PF01425.24 PF07719.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94559.t1	XP_003631114.1	94.1	594	9	1	1	568	1	594	0.00E+00	1081.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94559.t1	Q9MUK5	77.5	595	106	2	1	569	1	593	1.2e-252	873.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94559.t1	G7LE58	94.1	594	9	1	1	568	1	594	0.0e+00	1081.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene94559	MS.gene47674	PPI
MS.gene94559	MS.gene037397	PPI
MS.gene94559	MS.gene22216	PPI
MS.gene94559	MS.gene038240	PPI
MS.gene90875	MS.gene94559	PPI
MS.gene017032	MS.gene94559	PPI
MS.gene019162	MS.gene94559	PPI
MS.gene94559	MS.gene029150	PPI
MS.gene93101	MS.gene94559	PPI
MS.gene47995	MS.gene94559	PPI
MS.gene94559	MS.gene46950	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94559.t1	MTR_8g107280	94.108	594	9	1	1	568	1	594	0.0	1143
MS.gene94559.t1	MTR_8g107280	93.359	527	9	1	1	501	1	527	0.0	1006
MS.gene94559.t1	MTR_4g094205	48.080	599	278	4	1	567	5	602	0.0	550
MS.gene94559.t1	MTR_1g082750	49.490	392	197	1	42	432	32	423	2.60e-141	416
MS.gene94559.t1	MTR_4g126750	82.353	68	12	0	500	567	80	147	3.85e-31	119
MS.gene94559.t1	MTR_1g020070	86.567	67	9	0	501	567	157	223	1.35e-30	119
MS.gene94559.t1	MTR_1g084100	33.775	151	98	2	406	555	43	192	5.34e-19	90.5
MS.gene94559.t1	MTR_3g452710	42.105	114	66	0	453	566	11	124	6.32e-18	87.0
MS.gene94559.t1	MTR_3g452710	42.105	114	66	0	453	566	11	124	6.78e-18	87.4
MS.gene94559.t1	MTR_2g010180	34.694	147	94	2	40	185	198	343	3.42e-16	82.4
MS.gene94559.t1	MTR_7g059150	33.553	152	85	3	431	567	66	216	3.43e-16	79.3
MS.gene94559.t1	MTR_2g010180	34.694	147	94	2	40	185	198	343	3.63e-16	82.0
MS.gene94559.t1	MTR_2g010180	34.694	147	94	2	40	185	212	357	3.66e-16	82.0
MS.gene94559.t1	MTR_8g094740	37.692	130	81	0	56	185	221	350	1.06e-15	80.5
MS.gene94559.t1	MTR_8g094740	37.692	130	81	0	56	185	221	350	1.36e-15	80.5
MS.gene94559.t1	MTR_3g096980	34.615	130	85	0	56	185	220	349	1.70e-15	80.1
MS.gene94559.t1	MTR_2g090135	34.426	122	73	1	453	567	98	219	1.41e-14	74.7
MS.gene94559.t1	MTR_1g030300	41.228	114	63	2	453	565	181	291	4.94e-14	75.1
MS.gene94559.t1	MTR_8g080890	37.500	112	64	2	76	182	144	254	1.78e-13	73.6
MS.gene94559.t1	MTR_8g080840	38.393	112	63	2	76	182	143	253	5.66e-13	72.0
MS.gene94559.t1	MTR_8g080870	38.393	112	63	2	76	182	114	224	1.83e-12	70.1
MS.gene94559.t1	MTR_4g120360	37.705	122	70	2	452	567	344	465	2.38e-12	69.7
MS.gene94559.t1	MTR_8g097220	36.607	112	65	2	76	182	140	250	4.10e-12	69.3
MS.gene94559.t1	MTR_8g095600	35.780	109	65	2	79	186	266	370	9.90e-12	67.8
MS.gene94559.t1	MTR_3g048950	40.541	111	66	0	75	185	143	253	1.27e-11	67.8
MS.gene94559.t1	MTR_5g012030	29.630	135	89	1	421	555	366	494	1.92e-11	67.0
MS.gene94559.t1	MTR_8g095600	35.780	109	65	2	79	186	282	386	2.03e-11	67.0
MS.gene94559.t1	MTR_8g095600	35.780	109	65	2	79	186	266	370	2.06e-11	67.0
MS.gene94559.t1	MTR_4g074030	40.789	76	45	0	107	182	171	246	2.22e-11	66.6
MS.gene94559.t1	MTR_8g095600	35.780	109	65	2	79	186	266	370	2.39e-11	67.0
MS.gene94559.t1	MTR_4g132780	33.051	118	73	2	457	568	43	160	2.98e-11	66.6
MS.gene94559.t1	MTR_8g080880	36.607	112	65	2	76	182	143	253	3.90e-11	65.9
MS.gene94559.t1	MTR_8g011720	34.579	107	70	0	444	550	2	108	4.57e-11	64.3
MS.gene94559.t1	MTR_7g115280	33.628	113	74	1	448	560	331	442	9.08e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94559.t1	AT3G17970	63.604	566	180	1	28	567	23	588	0.0	717
MS.gene94559.t1	AT3G17970	62.548	518	168	1	28	519	23	540	0.0	645
MS.gene94559.t1	AT5G09420	48.993	596	272	4	3	566	6	601	0.0	583
MS.gene94559.t1	AT1G08980	47.608	418	192	3	36	426	4	421	3.66e-133	395
MS.gene94559.t1	AT1G56440	50.000	96	47	1	457	552	78	172	1.15e-21	98.6
MS.gene94559.t1	AT1G56440	50.000	96	47	1	457	552	88	182	1.43e-21	98.6
MS.gene94559.t1	AT2G42810	36.290	124	79	0	443	566	3	126	6.77e-17	84.3
MS.gene94559.t1	AT2G42810	36.290	124	79	0	443	566	3	126	7.16e-17	84.3
MS.gene94559.t1	AT2G42810	36.290	124	79	0	443	566	3	126	8.70e-17	83.6
MS.gene94559.t1	AT2G42810	36.290	124	79	0	443	566	3	126	8.70e-17	83.6
MS.gene94559.t1	AT2G42810	36.290	124	79	0	443	566	3	126	8.70e-17	83.6
MS.gene94559.t1	AT5G64440	34.615	130	85	0	56	185	215	344	4.66e-16	82.0
MS.gene94559.t1	AT1G04190	40.541	111	66	0	457	567	19	129	8.83e-15	76.3
MS.gene94559.t1	AT4G08320	40.351	114	64	2	453	565	175	285	3.94e-14	75.1
MS.gene94559.t1	AT4G08320	40.351	114	64	2	453	565	175	285	5.80e-14	74.7
MS.gene94559.t1	AT3G25660	36.429	140	85	1	50	185	115	254	1.62e-12	70.5
MS.gene94559.t1	AT5G07360	35.780	109	65	2	79	186	308	412	1.20e-11	68.2
MS.gene94559.t1	AT5G07360	35.780	109	65	2	79	186	308	412	1.20e-11	68.2
MS.gene94559.t1	AT1G12270	33.645	107	71	0	457	563	387	493	1.61e-11	67.4
MS.gene94559.t1	AT4G12400	39.806	103	62	0	453	555	2	104	2.04e-11	67.0
MS.gene94559.t1	AT4G12400	33.981	103	68	0	453	555	369	471	4.48e-11	65.9
MS.gene94559.t1	AT4G12400	39.806	103	62	0	453	555	2	104	2.47e-11	66.6
MS.gene94559.t1	AT4G12400	33.981	103	68	0	453	555	369	471	5.61e-11	65.5
MS.gene94559.t1	AT1G62740	32.710	107	72	0	457	563	386	492	2.55e-11	66.6
MS.gene94559.t1	AT3G16760	36.066	122	72	2	452	567	352	473	5.57e-11	65.5

Find 146 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAATCTTCGAACAATTTA+TGG	0.057750	4.1:-291754	MS.gene94559:CDS
ATCTCCTTTCCACCTAATTT+TGG	0.162409	4.1:+285373	None:intergenic
TCCATTTGCAGACATGAATT+TGG	0.175139	4.1:-288796	MS.gene94559:intron
CTGCCACTGCTTATCTTTGT+AGG	0.184219	4.1:+284026	None:intergenic
AGTGGCGAAAATAAGCATTT+TGG	0.234600	4.1:-291198	MS.gene94559:CDS
TCTTAAGGACGAAGGAATTT+TGG	0.240223	4.1:-285422	MS.gene94559:intron
AGCAATAAGTCTTGACAAAA+AGG	0.253299	4.1:-283805	MS.gene94559:intron
CTAAGTATTGCTAGCATATC+AGG	0.267474	4.1:-285313	MS.gene94559:CDS
GAACAATTTATGGGTATTAC+TGG	0.271346	4.1:-291744	MS.gene94559:CDS
TTGTAGAAATTGCATAATAT+TGG	0.276970	4.1:+289091	None:intergenic
ACGCAACATACTACAGTAAC+AGG	0.292638	4.1:-283957	MS.gene94559:CDS
TTATGGGTATTACTGGGTTT+GGG	0.305409	4.1:-291737	MS.gene94559:CDS
CAAGGAGAAATCAACTAAAT+TGG	0.305680	4.1:+291101	None:intergenic
GTTTCTACTCTTGTTCAATC+TGG	0.307595	4.1:-291376	MS.gene94559:CDS
GCTGATCCTCCTCCAAAATT+AGG	0.309333	4.1:-285385	MS.gene94559:CDS
TTTATGGGTATTACTGGGTT+TGG	0.318931	4.1:-291738	MS.gene94559:CDS
TTGCAACAATACCTTCTCTT+TGG	0.324011	4.1:+284807	None:intergenic
CACCACCATAACGAGCTATC+AGG	0.326660	4.1:+284950	None:intergenic
AATTTAGTTGATTTCTCCTT+GGG	0.328288	4.1:-291099	MS.gene94559:intron
GCTGGATTCTGTGGGATACT+TGG	0.331180	4.1:-290027	MS.gene94559:CDS
CCCAGCTTGAAGGCGTGTTC+TGG	0.331605	4.1:-289161	MS.gene94559:CDS
TTCTCGACCACCTCTCTTAC+GGG	0.335821	4.1:-291330	MS.gene94559:intron
CAATTTAGTTGATTTCTCCT+TGG	0.341978	4.1:-291100	MS.gene94559:CDS
AGAGGACGCCATTGATTTCA+TGG	0.348851	4.1:+291775	None:intergenic
TCAATCTTCGAACAATTTAT+GGG	0.360486	4.1:-291753	MS.gene94559:CDS
CCAACTCTACGCAGTATATC+AGG	0.360852	4.1:+289544	None:intergenic
ATTACAGGCGTTGATACTTC+TGG	0.364126	4.1:-290066	MS.gene94559:intron
TCGTGCGTCCTTGCCCATTC+CGG	0.364934	4.1:+291424	None:intergenic
CGTGTCTACGTTTGGTCATC+CGG	0.366026	4.1:-291443	MS.gene94559:CDS
TCACATGGTGCTGTTTCTCA+TGG	0.367567	4.1:-289994	MS.gene94559:CDS
CATCGGCAATAATTATTTGC+CGG	0.373526	4.1:+289479	None:intergenic
GTTCTCGACCACCTCTCTTA+CGG	0.377000	4.1:-291331	MS.gene94559:CDS
ACAAACAGATTTGACATAGA+AGG	0.383943	4.1:-291469	MS.gene94559:intron
TCCTGTCAAGAGGAACATTT+AGG	0.392168	4.1:+289443	None:intergenic
GAAACAGCTGGGTGAAGATC+AGG	0.407748	4.1:+288739	None:intergenic
CAAACTGTGAAAGAGGATTT+CGG	0.423798	4.1:-291677	MS.gene94559:CDS
CACATGGTGCTGTTTCTCAT+GGG	0.425188	4.1:-289993	MS.gene94559:CDS
AGGTACAGCTAGAGAGATGC+TGG	0.426080	4.1:-283334	MS.gene94559:CDS
ATCTAAAGTTCCCAGCTTGA+AGG	0.426528	4.1:-289171	MS.gene94559:CDS
ATTTGATTCCATGAAATCAA+TGG	0.431972	4.1:-291783	None:intergenic
GTTTCCCTGATAGCTCGTTA+TGG	0.438795	4.1:-284955	MS.gene94559:CDS
TACTTGAGCCAACCAACAAA+AGG	0.440758	4.1:-282848	MS.gene94559:CDS
CGGGGATAGCGGGATTGGTA+GGG	0.444809	4.1:+291172	None:intergenic
TGTTGGTTGGCTCAAGTACA+AGG	0.448590	4.1:+282853	None:intergenic
ATCGGCAATAATTATTTGCC+GGG	0.453217	4.1:+289480	None:intergenic
CATACCCGTTTCAACAAGTC+TGG	0.453788	4.1:-289965	MS.gene94559:CDS
AGGGTGCTTGTAAAAGTATA+TGG	0.454570	4.1:+289521	None:intergenic
GCCAGAACACGCCTTCAAGC+TGG	0.458558	4.1:+289160	None:intergenic
TACGTTTGGTCATCCGGAAT+GGG	0.460111	4.1:-291437	MS.gene94559:CDS
GGGGATAGCGGGATTGGTAG+GGG	0.463096	4.1:+291173	None:intergenic
GGAGGGGTGAGAGTGCCTGC+TGG	0.464288	4.1:-290045	MS.gene94559:CDS
GGTAGACGAGCGGGGATAGC+GGG	0.466037	4.1:+291162	None:intergenic
GCGGGGATAGCGGGATTGGT+AGG	0.471210	4.1:+291171	None:intergenic
GCTATCCCCGCTCGTCTACC+TGG	0.473289	4.1:-291159	MS.gene94559:CDS
TTACTGGGTTTGGGTTTAGC+AGG	0.473413	4.1:-291728	MS.gene94559:CDS
CTACGTTTGGTCATCCGGAA+TGG	0.475998	4.1:-291438	MS.gene94559:CDS
ACCTAAATGTTCCTCTTGAC+AGG	0.479448	4.1:-289444	MS.gene94559:CDS
GCTTAAACAAACTGTGAAAG+AGG	0.483235	4.1:-291684	MS.gene94559:CDS
AGAGTGCCTGCTGGATTCTG+TGG	0.483495	4.1:-290036	MS.gene94559:CDS
GAGCCGCCAGGTAGACGAGC+GGG	0.484166	4.1:+291153	None:intergenic
TTTCAGGTCACAGTACCATT+GGG	0.485220	4.1:-285003	MS.gene94559:intron
GAAGGACACGTGTCTACGTT+TGG	0.487354	4.1:-291451	MS.gene94559:CDS
GCTATCAGGGAAACTGAAAC+AGG	0.492446	4.1:+284964	None:intergenic
GGCTCTGATAATCCTGTGAT+AGG	0.495225	4.1:+285350	None:intergenic
TACTACAAAGAAGCAATTGA+TGG	0.495869	4.1:-283309	MS.gene94559:intron
CTCTTGTAGACTTGTATACA+TGG	0.501692	4.1:+284900	None:intergenic
TGCAATTGTGCTGAAACAGC+TGG	0.503072	4.1:+288727	None:intergenic
GCACCACTAGAGGAGCCGCC+AGG	0.504143	4.1:+291141	None:intergenic
ACATGGTGCTGTTTCTCATG+GGG	0.504209	4.1:-289992	MS.gene94559:CDS
GCTCAAGCGTATCTTGAACT+TGG	0.504250	4.1:-283934	MS.gene94559:CDS
ATATGCTAGCAATACTTAGC+AGG	0.504439	4.1:+285317	None:intergenic
GTGTCCAGACTTGTTGAAAC+GGG	0.506010	4.1:+289961	None:intergenic
CTTCAGCTGCTGAAAGATTG+AGG	0.506401	4.1:-282821	MS.gene94559:CDS
GAGAACGGTAGTAGCAATAC+AGG	0.511457	4.1:+291348	None:intergenic
ACGAGCGGGGATAGCGGGAT+TGG	0.514474	4.1:+291167	None:intergenic
AAGCAGTGGCAGAAAGCCAT+TGG	0.520099	4.1:-284015	MS.gene94559:CDS
CTACCTGGCGGCTCCTCTAG+TGG	0.521414	4.1:-291144	MS.gene94559:CDS
TCCTCCAAAATTAGGTGGAA+AGG	0.522866	4.1:-285377	MS.gene94559:CDS
GTAAGAGAGGTGGTCGAGAA+CGG	0.527108	4.1:+291333	None:intergenic
ATCAAGAAATGTTGTTAGCA+AGG	0.529714	4.1:-284848	MS.gene94559:CDS
GGAGGAGGATCAGCTACAGT+AGG	0.529844	4.1:+285394	None:intergenic
AGTGTCCAGACTTGTTGAAA+CGG	0.533742	4.1:+289960	None:intergenic
TTGTAATGCAACGCAGTCCC+CGG	0.534546	4.1:-289498	MS.gene94559:CDS
CCAGAACACGCCTTCAAGCT+GGG	0.534601	4.1:+289161	None:intergenic
TAGCATATCAGGTTGCTGTC+AGG	0.535420	4.1:-285302	MS.gene94559:intron
TGATGTATCCGAAGTGGAGA+TGG	0.539165	4.1:-288695	MS.gene94559:CDS
CTTGGCTTTCGACCTTCACA+TGG	0.544613	4.1:-290009	MS.gene94559:CDS
CGCTGAGATTGCCAAAGAGA+AGG	0.549950	4.1:-284818	MS.gene94559:intron
TGAACAAGAGTAGAAACTGC+AGG	0.556714	4.1:+291382	None:intergenic
ATATTATGCAATTTCTACAA+AGG	0.559204	4.1:-289088	MS.gene94559:intron
CTTTATTAATTCAGCATCAG+TGG	0.560639	4.1:-291216	MS.gene94559:intron
GTATACAAGTCTACAAGAGC+AGG	0.561649	4.1:-284896	MS.gene94559:CDS
GCAGGGGAAGTAGAAGAAGC+AGG	0.570121	4.1:+291400	None:intergenic
TGGAAAGGAGATCCTATCAC+AGG	0.570732	4.1:-285362	MS.gene94559:CDS
TTGGTCATCCGGAATGGGCA+AGG	0.571161	4.1:-291432	MS.gene94559:CDS
AACAATTTATGGGTATTACT+GGG	0.571256	4.1:-291743	MS.gene94559:CDS
CCTGATATACTGCGTAGAGT+TGG	0.571803	4.1:-289544	MS.gene94559:CDS
TCTCTCTAGCTGTACCTCTA+CGG	0.572236	4.1:+283340	None:intergenic
ACTTGAGCCAACCAACAAAA+GGG	0.575182	4.1:-282847	MS.gene94559:CDS
TACTTAGCAGGCTAAATGCA+CGG	0.575999	4.1:+285329	None:intergenic
AAGCGTATCTTGAACTTGGA+AGG	0.576073	4.1:-283930	MS.gene94559:intron
AAAATTTGATGTATCCGAAG+TGG	0.576094	4.1:-288701	MS.gene94559:CDS
TTGTACAATCTGCCTCAGCT+TGG	0.578994	4.1:+283829	None:intergenic
AGTATCCCACAGAATCCAGC+AGG	0.584224	4.1:+290030	None:intergenic
GGGACTGCGTTGCATTACAA+AGG	0.587842	4.1:+289501	None:intergenic
TAAATTGTTCGAAGATTGAG+AGG	0.588658	4.1:+291757	None:intergenic
GATCCTCCTCCAAAATTAGG+TGG	0.588943	4.1:-285382	MS.gene94559:CDS
ACTTGAGAACTCCTGTCAAG+AGG	0.590616	4.1:+289433	None:intergenic
GGACTGCGTTGCATTACAAA+GGG	0.592007	4.1:+289502	None:intergenic
AGCTGTACCTCTACGGAAGT+AGG	0.593410	4.1:+283347	None:intergenic
TCAACAAGTCTGGACACTGT+TGG	0.595162	4.1:-289955	MS.gene94559:intron
ACCACCATAACGAGCTATCA+GGG	0.599413	4.1:+284951	None:intergenic
TAGGTGTTGAAAACAGTCAT+CGG	0.600416	4.1:+289462	None:intergenic
GAGTGCCTGCTGGATTCTGT+GGG	0.604435	4.1:-290035	MS.gene94559:CDS
GGTACAGCTAGAGAGATGCT+GGG	0.607880	4.1:-283333	MS.gene94559:CDS
CATGGTGCTGTTTCTCATGG+GGG	0.611160	4.1:-289991	MS.gene94559:CDS
GAGGAGGATCAGCTACAGTA+GGG	0.611389	4.1:+285395	None:intergenic
GAACAAGAGTAGAAACTGCA+GGG	0.615180	4.1:+291383	None:intergenic
TCCCTGATAGCTCGTTATGG+TGG	0.617870	4.1:-284952	MS.gene94559:CDS
TCTTGACAGGAGTTCTCAAG+TGG	0.620973	4.1:-289431	MS.gene94559:CDS
AGCTTCAGTATAAAATCCAA+TGG	0.622577	4.1:+283999	None:intergenic
CTTAATGCTCTTAAGGACGA+AGG	0.626087	4.1:-285430	MS.gene94559:intron
TTGGACATGTGCATGATGAG+TGG	0.628329	4.1:-288777	MS.gene94559:CDS
ACAGGCGTTGATACTTCTGG+AGG	0.628596	4.1:-290063	MS.gene94559:intron
CAGGCGTTGATACTTCTGGA+GGG	0.630046	4.1:-290062	MS.gene94559:CDS
TGAGAAACAGCACCATGTGA+AGG	0.630301	4.1:+289997	None:intergenic
AACGAACGAACCCGTAAGAG+AGG	0.632150	4.1:+291320	None:intergenic
GTTGGTTGGCTCAAGTACAA+GGG	0.632408	4.1:+282854	None:intergenic
CTTCTCTTTGGCAATCTCAG+CGG	0.638681	4.1:+284819	None:intergenic
CGCAACATACTACAGTAACA+GGG	0.640100	4.1:-283956	MS.gene94559:CDS
AGCCGCCAGGTAGACGAGCG+GGG	0.644394	4.1:+291154	None:intergenic
AGGTAGACGAGCGGGGATAG+CGG	0.644820	4.1:+291161	None:intergenic
TAAATTGGCAGCAACAGCAA+CGG	0.645173	4.1:+291116	None:intergenic
GAACGAACCCGTAAGAGAGG+TGG	0.650427	4.1:+291323	None:intergenic
TACTTGTCATAAAATCCCAA+TGG	0.651161	4.1:+284988	None:intergenic
AAGCCTACAAAGATAAGCAG+TGG	0.651878	4.1:-284029	MS.gene94559:CDS
GCAATTGTGCTGAAACAGCT+GGG	0.652367	4.1:+288728	None:intergenic
AGGCGTTGATACTTCTGGAG+GGG	0.657859	4.1:-290061	MS.gene94559:CDS
ATCCAGTTATCTCCAAGCTG+AGG	0.661684	4.1:-283841	MS.gene94559:intron
AGCAACGGCAGCACCACTAG+AGG	0.662776	4.1:+291131	None:intergenic
TCGGCAATAATTATTTGCCG+GGG	0.663537	4.1:+289481	None:intergenic
GTGAAGGCCTACTTCCGTAG+AGG	0.686734	4.1:-283354	MS.gene94559:CDS
GGAGCCGCCAGGTAGACGAG+CGG	0.687249	4.1:+291152	None:intergenic
AACAAGAGTAGAAACTGCAG+GGG	0.699488	4.1:+291384	None:intergenic
ATCCCCGCTCGTCTACCTGG+CGG	0.715764	4.1:-291156	MS.gene94559:CDS
ACTGAAGCTATTAAACTCTG+CGG	0.727513	4.1:-283985	MS.gene94559:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	282806	291795	282806	ID=MS.gene94559
chr4.1	mRNA	282806	291795	282806	ID=MS.gene94559.t1;Parent=MS.gene94559
chr4.1	exon	291656	291795	291656	ID=MS.gene94559.t1.exon1;Parent=MS.gene94559.t1
chr4.1	CDS	291656	291795	291656	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	291331	291483	291331	ID=MS.gene94559.t1.exon2;Parent=MS.gene94559.t1
chr4.1	CDS	291331	291483	291331	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	291100	291224	291100	ID=MS.gene94559.t1.exon3;Parent=MS.gene94559.t1
chr4.1	CDS	291100	291224	291100	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	289956	290081	289956	ID=MS.gene94559.t1.exon4;Parent=MS.gene94559.t1
chr4.1	CDS	289956	290081	289956	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	289401	289583	289401	ID=MS.gene94559.t1.exon5;Parent=MS.gene94559.t1
chr4.1	CDS	289401	289583	289401	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	289089	289233	289089	ID=MS.gene94559.t1.exon6;Parent=MS.gene94559.t1
chr4.1	CDS	289089	289233	289089	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	288639	288807	288639	ID=MS.gene94559.t1.exon7;Parent=MS.gene94559.t1
chr4.1	CDS	288639	288807	288639	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	285303	285437	285303	ID=MS.gene94559.t1.exon8;Parent=MS.gene94559.t1
chr4.1	CDS	285303	285437	285303	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	284819	285019	284819	ID=MS.gene94559.t1.exon9;Parent=MS.gene94559.t1
chr4.1	CDS	284819	285019	284819	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	283931	284058	283931	ID=MS.gene94559.t1.exon10;Parent=MS.gene94559.t1
chr4.1	CDS	283931	284058	283931	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	283806	283857	283806	ID=MS.gene94559.t1.exon11;Parent=MS.gene94559.t1
chr4.1	CDS	283806	283857	283806	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	283310	283379	283310	ID=MS.gene94559.t1.exon12;Parent=MS.gene94559.t1
chr4.1	CDS	283310	283379	283310	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1
chr4.1	exon	282806	282888	282806	ID=MS.gene94559.t1.exon13;Parent=MS.gene94559.t1
chr4.1	CDS	282806	282888	282806	ID=cds.MS.gene94559.t1;Parent=MS.gene94559.t1

Gene Sequence

>MS.gene94559

ATGAAATCAATGGCGTCCTCTCAATCTTCGAACAATTTATGGGTATTACTGGGTTTGGGTTTAGCAGGAATTTATGTTTTAACGAGAAAGCTTAAACAAACTGTGAAAGAGGATTTCGGTGCTTTCATTCAAAAGCTTCAGTTGCTTCCTCCTCCGCCTCCTGCTCCTCCCAAAGCTCCTCATCCACTCACTTCTCTCACTTTCGCCATCTCCGACTTGTATGTCTTTTCTTTTCATTTCGTTTCGTTTCGTTTCTAATAACCTAATAACAGGGTATGATTGAATTGATTTGAATTGAATTTGAACAAACAGATTTGACATAGAAGGACACGTGTCTACGTTTGGTCATCCGGAATGGGCAAGGACGCACGAACCTGCTTCTTCTACTTCCCCTGCAGTTTCTACTCTTGTTCAATCTGGTGCTACCTGTATTGCTACTACCGTTCTCGACCACCTCTCTTACGGGTTCGTTCGTTCGTTCCTCCAATAAACCCTACCTACCCTTTTTTACTTCATCATATAATTATCGTTCGTAATCGTAATAATAATTGTTACTTCTTTATTAATTCAGCATCAGTGGCGAAAATAAGCATTTTGGAACCCCTACCAATCCCGCTATCCCCGCTCGTCTACCTGGCGGCTCCTCTAGTGGTGCTGCCGTTGCTGTTGCTGCCAATTTAGTTGATTTCTCCTTGGGTCAGCTTCTTCTTATATCTTTTTACTACTCATTTATTAACATGCTTCAAACATATTTGTTTAGTAGCCAGTCAATTATGGTTTCTAGTACGCATGTTCTAAACTAACCTATTACTTGTTCAATGGATGCCAACTAAACTTCCGATGATAGCTATCAAAATTTATTATTAGTAATTTGAAGCTACCATACACTCAATTTGACCAGTGAAAGATCTTCTTTTCATAACCAGACATCAAGTATCTTGACTCCATACTACAAAATGATAGGGAGATGGGATGTGTAAAACTATGGGGTGATTCAACCGGTTCAGATTGGGAATGAAAAGGATGTATTATTATTATTATTATTGAATGAACAACAACACACAAACTCATGGATTTTCAAAACAAAATCCAGACTTCCCAATTGCCTAAAAGGCTCCAACTCTAATCAATCGACAAGATATATTATTCCCTAAGGCTTTGTTTGGGAGTTTGGAGGGGAGGGGAGGGGAAGGGAAGAGGGGTGAGAAATATAGGGGAAAATTGAGAAATCTTTCACATTTTTTTTAAGAGTATTTTTTTAGAGTGTGATAAATTAATACTTATGATTAATATATTTTTATTTTTAAGTATATTATAACAACATAGATCAAATTTGAATAATTCATATAAACCCTCTCAAACCCCCCAAAACCTTCCTCTGATACAATGTCTTAGTTCCCCCAAATGAGGAGGGTTTTATAATTTGAAGAAAAGTTAACCCCCCAATGTCCTCTATTTCTTTCACTTACTCCTTCCTTTTTTCCCAAGCCCTCCCCTCCCTTCCCCTCCAAACTCGCAAAACAAAGCCTAAGGCTCACCTCTTAATAAATTTTAAGGAAAGATGCTTCTGTAACTTTGAATGAATTCATTCTTGTTGCCACATAATGCGTACTTATATATGTCTGCGTAATATATTTATAGTTTTCTGGTTCAATATCACTAACATGTTGACATTTGAATGTATCTCATACGATTGCACCATTGTGTATTACAGGCGTTGATACTTCTGGAGGGGTGAGAGTGCCTGCTGGATTCTGTGGGATACTTGGCTTTCGACCTTCACATGGTGCTGTTTCTCATGGGGGAATCATACCCGTTTCAACAAGTCTGGACACTGTTGGTATGATTGATTCGTTTATTTATTCTGATTTAAAATATGGGCTTCTGTGCTTAAGTGGCTGCTTGATTTATTTATTTTGAACGATTAGTATATTTGCATCGTATAAAGATCAAGTGCAAATTTGGATTGATTACTTTTTAGCAGCTTTTTTAGATGCTTTCTTCGACAATATTTTGATTATAAACAGTTGAACACAATGGGTAAGCTAATACACACCGAGAACAGGATAAAAAGATGTTCTATTACAGCCGTTTTATTTAAACCTTCTTCTTATAGTTGCTGGCACTTTCTTAGCTTTTATATGCAGATGCTATGCTTCTGCCATCTGCTTCAGTTTTGAAACTCATTTTACCTCTGAAATTCTCTTTTCAGGTTGGTTTGCAAAGGATCCTGATATACTGCGTAGAGTTGGCCATATACTTTTACAAGCACCCTTTGTAATGCAACGCAGTCCCCGGCAAATAATTATTGCCGATGACTGTTTTCAACACCTAAATGTTCCTCTTGACAGGAGTTCTCAAGTGGTGATCAAAGCCACCGAGAAGCTTTTTGGAAGTATGTTCTTTTGTTGTGTCCTTTGCAGTTTAATATAATCCCTATCGTTTTGGTTATCATCGAAAGCTCCTGCCTCAGTTTGTGTGCTGGCTTGTGATTTTTGAATAACCTGTGCTTGTTCTTACTATCGCATTCTGTTATTTTTTTTAATTATCAATTATCTTCAGAGCAAGTATTGAAGCATATAAATCTTGAAGACTATATAAGATCTAAAGTTCCCAGCTTGAAGGCGTGTTCTGGCCAAAAAGCAAATGGTGAACTGAAATCTTCTTCGTTGATATTGCTTGCCAATATTATGCAATTTCTACAAAGGTTAGTTGGAAATATCATTATAATTTGCAGTTTTACGGGAAAACTTGAATGGAAGTTTTTTATTTTATTTTTAAACTTGTGGTTTGGTAGAGTTGTTTTACATTTAGCAGTTTTGTTGTGTTTTGATCTATTCACTTTGTATCATGTCATAACTCATAACTTCTCCTTTTCAATTAGTATTCCTCATTGCGGGTTTACTTAATTTATACTTGTTGTGTATTATGACCGTTTGCGGAAGCCTTGAATAATAATGTGATATTTTTTTGCCACTCCATTTGCAGACATGAATTTGGACATGTGCATGATGAGTGGATGAGCATAGTAAAGCCTGATCTTCACCCAGCTGTTTCAGCACAATTGCATGAAAAATTTGATGTATCCGAAGTGGAGATGGAAAACTCTAAATCTGTTAGAAGTGAGATGCGTGTTGCTGTAAATTCACTTTTGAAGGTAATAATTTTTTTTTACTCTTCCCCTATATATCTTTGGGCTGTGTTTGGTAACACGAATAAGCTAGCTTATAAGCTTGTTTTGATATACTAAGAGCATGTTTGGCCCAGCTTTTTGGGGACTTATTATCTCCTTGTAAGGAGAAGTAGGGCCAAACATAATTTAGCCTAAGCTCCTTAACTAAAAAGTTGCTTTTTTATAAAGAAGAAATATAAGAAAAAAGTACTTTTAAAAGAAGGTGATGAGAGTTACTTTTACAAAACACGCTCAACTTATTTTTAGAGCTCTCCCCTTCGAATCCCATTGAGGACACAACCTATGTTTTATTTTAATTTTTTTGACGTTGTTTCTCCAATTGCTAATGTCATATGCTCTCCAATTGCTTATGTATTTTTTATTTTATTTTTAGGGGGCCGTTATCATTTTTTGGTTAATTGCTATTGGTACTTTTTTTTATTGACAAATGATATACTGGTTCGTAAATAGTATTTCTCTAACTATATCACACTTACTTATGTGCTCTTTCAAAGAAAATATATGAATTAAATTTAAAGGTTCATATTTATATATTTAATCAAGAATTATTTATTCAGGCCTCTAAATTTTATTAAAAATATAGTTTTAAAAGAATTTTTCTCTCATTATTAAATATTTATCTTTTAAATCATAACAAGGTATTTTTATCCAAACAAAACAGAAAAAAAATCATCAAAAAAGTTATCATTTATTGAATATCATAAATATTATTTAATCTGATAATGGATGAATTTTTTTTATAAATCTTATTCATTTTTTAAATATTTATAATGATTTATAATAAAATATGAGCTTGTTAAAATAAATTGATTTTATTTGAAAATATTATCAAGAACTTTAACATAAAAAATTTGAAATAAAAAACAATGTTTACCAAACAACTTTAACTTTATCTTTTAAGCTGTTATTTTTAACTTTAAGTTTAAAGTAGCTTATATGCCTAAAAAAAGCTGGGCCAAACGCAACCTAAGATGTGTTTGGTAAAAAGCTTTTTCCACTAGCTTATAGCGTTTTTTGAGAAGCTATTTCAAGTAGCTTTTGAGCTTATAGCTGGTAGCTTTTTATATTTTCTTCCAATTTTAACCCTATTAATTTAATTATTTTTTAATAAAACATGTTCATGGCTTAAAAAATAACTGCCACGTTCATAGCTTATTTTTAATAAAACAACTGCCACATTTTTTCCTTTGAAAAAAAACTTCCATGCTTTATTTTATTTTTTAAGGAACGCTTTATATATATATATATATATATATATATATATATATATATATATATATATATATCTTAAAAGAAATGCTATATATTCTTGCTTACATTTTTTTCCACCTTCCTCTTATTTTTGGGATTCATAAAAGTAGAGATCAATGAGCTTTTGGCCTCTTTTGTATCAAAAAAAAAAAAAAAAGCAGAGATCAAAATTGTCGGTGAAATTTTTTTGGAGACTAAAACCAAAAGTTTGAATATTTATAGGGATCCAAAACATAACTAACCATAAATCAAAATATTAAAAAATAAATACATCAGATACATTAGAAAAAATGTGTGTGTGTGTCTATATATATATATATATATATATATATATATATATATATATATATATATATATATAGAGAACATATACTATGAGAATGCCATTTCTATATGAGAATGATGAGAATGAATAAGAGCCATCAAATTAAAAAGGAATGGTTGAGATTAAAACATTCCTAAAGTGAGTCATTTATCATTGACACATTATATAACTGTATTGCGTCATTAATTAGTATTCCTTTTTTCAACATTACTAAAATAGAATTTTGAATTCCTCATGGATCAAGGCATCAAGCTTCTGTAATACATTTGCTCAAAGAATAAAATAAAAAAAAATGCTTCTTAGAACATCTTTTTTTTTTTTAAAGATAAACTTAGAACAATGAAATATGAGTATCCAGCTACTTCATGGATCAGACTGGGATCACGACAAAATACAAAATATGGGAACAAAAGACAACAATTTAGAAGAAAAAATTGAAATCAAAGGGATAAATATGTATTTTTATGGTGTGATAAGATCCAAATTGTGAAGGAAACCAGTGAAATATTAATCTGTTGAAATGGAAATAAGGAATTTTTCGATGGAGAGAGATGAAGACCATGGAGAGAGACACTGATTAGGAATAAATGAATAATAATGACACCTTCTTAAAAAAGGAATAATCTTGATACAATAAAAATAATAATTATATAATGATGTCAATGACACATGACTCACTTTAAGAATATTTTAATCTCAACCATTCCTTTTTAATTTGATGGCTCTTATTCATTCTCATCATTCTCATATAGAAATGGCATTCTCATAGTATATGTTCTCTATATATATATATATATATATATTATAACTTAAAAAAAATGTCCTTTTATGTCATTTTATACTTATCAGCCACTTCAAGAACTAATTTTAATAAACCAGCTTTTCAGCTATAAGCTATCAGTTATAAGACATCAGCTATATGCTAGCTTTTCAGCTATCAGCTAGCTTATAGCTTATTTTTACCAAACACACAGATCCTTTTGATTTGAAATCAATATTCATGCTGCCTCATTCCAATGTTAGCTACATTTTTTAAAGCGTTTATTTTACCAGTGGTTTATCCTTCTTGCTTTCTTAAACAAACAGAAGAGTACATTCACAGATCATAGTGGGTAGATTTTAATGTGATTGTTAAAGAGTTAAAAGCTGTACTGCATTTTAACAAACTTATGGTTTTGGTGAATGGAACATGGTTAATTGCTTTTGTATGGAGTACCTTTAATTTGTATTTCCAACTCTCTTTATAAAAATTAACCAATATATTCACAGACAAGCAAAGTGTGTTGTGGGTGGATTATAATATCTGGTTGTTAAGGGTTAAAATTTATGCTGAATTTTACAAATTTATGGTGAACGTAACATTGATATTGAATGGGCAAAACATTTCTAAATAAGTTACTGAGAATATACTGCAGAACACATGCAGAAAATTCTTTCAAAGATGAAAGTGGATATTTTCAATCGATTTATGGTTTTGAATATATTAATTTTTATAAGTTTTCAATTTAGTATTTTAGTAATTTATATTCTGATAAGGGAAACATAGTTGTTCAGAATTTAATACATTTTAATGGCTTAATGCTCTTAAGGACGAAGGAATTTTGGTGATCCCTACTGTAGCTGATCCTCCTCCAAAATTAGGTGGAAAGGAGATCCTATCACAGGATTATCAGAGCCGTGCATTTAGCCTGCTAAGTATTGCTAGCATATCAGGTTGCTGTCAGGTAAAGATTTCACCCTCATGAAACTTGCTTAATGTTATCCGTTTTTTTTTGTTTTGTTTATATTTGTGACTGTTTTGTCTGTATTTTCAGAATTTGTTTATATTTCATGGAAACTTGTTTCCTCAATCAATATCATTGCACTCTTTGTTTGATTTTCATATGTGATAGAATTCAAATTACCAATATGCACCTGATGGGTATTTTATGAAATATATGATAGGCACTCTTTCATAATTGTGATTGTAATGACTTTTGTTCATATTCTCCTAATTTATCTTTTCAGGTCACAGTACCATTGGGATTTTATGACAAGTATCCTGTTTCAGTTTCCCTGATAGCTCGTTATGGTGGTGATCGCTTTTTGCTTGACACGCTAAAGACCATGTATACAAGTCTACAAGAGCAGGCTGATATTGCTGCCACAAGTAAAACATCAAGAAATGTTGTTAGCAAGGAACAATCCGCTGAGATTGCCAAAGAGAAGGTATTGTTGCAAATGAATCAGATTCAGCAGTCACCTATTTTTATTTTGTAATTGTTCTGTGTAACTGGCATGGTTATAGTTCTAAATCACCAGTGACGTTTAAAATTATAATATTATAAAACCATCTACGACAACAACAACCAAGCCTTTAGATCTTTCTTAATAAGTTCCCTGATAGTTTTTCTAGGTCTTCCTCTGCCTAGAGTGATTTGATGACCCTCCATCTGATCTACTCTCCTTACTACATAATCCATAGGTCTTCGTTGTAGATGCCCAAACCACCACCATCTTTTCTACGATAGGTGCTGCCCCAACCCTTTCTCTGATGTTGTCATTTCTAATCCTATTTTCTCATCTGTGCTGCGCTTATTTTATTCTCATGTTGGTTCTTTACCGCCCAACATTCTGTCCCATACAACATCGCAGGTCTTACATCTGTTTGATGGTTTACATCTCCTATTTCTCCACCGTTTTGTACTATGGACCCAGGGTATTTAAATCGTGTAACTTGTGGTATGACATGATCTCCAACTTTACATCAGGGATAACATTACATTTGGTGTCTTTCATAACTTTCTCTATGCAAATGTAGGGATGATGCTTTTTTTTAGGTGTTGTAATTTATCCTTGAACTTCCCCTATAATTTAAGCTGCCCTTTTTATTAAGATGCATCTGATAGTTTTACTCGTTAATTCTCAGTTCCTTTTGTGTTTGTTCTCCTCACTCATTATTTTTGGGGTTCATCGTTCAATGATTCAGGGAAATCAAGCCTACAAAGATAAGCAGTGGCAGAAAGCCATTGGATTTTATACTGAAGCTATTAAACTCTGCGGCGATAACGCAACATACTACAGTAACAGGGCTCAAGCGTATCTTGAACTTGGAAGGTAGCGTTTCATTCTTTTGCTATTTCTCTCTGAGTTTCCTGCAAACTAATGGATTTATTCTTTGTTAATCCAGTTATCTCCAAGCTGAGGCAGATTGTACAAAAGCAATAAGTCTTGACAAAAAGGTAACTTTCATCTTCGAAGAACTTTATCCTTTGAAGTTAATGTCATTGGTTTGAATGATGGCTGTCATTTTAGACTATGACAAAATCTTGTCATCATTACAAATATACATGTTTGTTGAAAATGAAGACTTAATAATTGAATTGCTATTCAGTTTGTTAAGAACACATACATTTTGTGTTGTATTGGATCCAACAATTTTTGCCCCTAGATTAATATAATTTCTATACGACACTCAGAATAAAACAAAGAGAAAAGGAGAAAGAAATAGATTTATGGAAAAAATATTTCCTGCTATTGTTCATTCTTTACTCTTCATACAAAATCCAATTAAATAGTTCATATACGTGATTATTGTATGTATTTTTGAATGAGATAGCCATGGGCATTTTTCAGTAACGTCCTCTCTTTATTCTTGTTTTAAACAGAGTGTGAAGGCCTACTTCCGTAGAGGTACAGCTAGAGAGATGCTGGGCTACTACAAAGAAGCAATTGATGGTAAGATCTTATATTCTCAAATTTGTTTACATTTTCTCATTTCCTCTAGAAAATTGAAGAGGTTCTCAAACTCGAGAGCCAATCCGAAGCTATTAATAGCTAACATGATTTGCCGCACATTTCCTTTTTGTTGAAGTTCATTTGGTTCTAAAATTATCTAAAATATATAAGATTTCACTTGGCTCTTGCTTATTGGGCTTAAGTTGATCCATTGACCATATTTAGCTGATTTCCATTTGTGGGCTTCTAGTATTTTTTCTTTTAGTTGGTTATGGGAACTCAAAAATCATGTTTTGATGATTATTAGAGGACTGTTCAAAATTCAAATGTTTGAATAAGACAACTGCATATTGACAGGGTTTACATTCTGTTATCCTCATCAGTTTCAAAAGCATACACTAATGAAAGATTTGGCATACAGATTTTAAATATGCCCTTGTACTTGAGCCAACCAACAAAAGGGCTGCTTCAGCTGCTGAAAGATTGAGGAAACTATTTCAGTAG

Protein sequence

>MS.gene94559.t1

MKSMASSQSSNNLWVLLGLGLAGIYVLTRKLKQTVKEDFGAFIQKLQFDIEGHVSTFGHPEWARTHEPASSTSPAVSTLVQSGATCIATTVLDHLSYGISGENKHFGTPTNPAIPARLPGGSSSGAAVAVAANLVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHGGIIPVSTSLDTVGWFAKDPDILRRVGHILLQAPFVMQRSPRQIIIADDCFQHLNVPLDRSSQVVIKATEKLFGKQVLKHINLEDYIRSKVPSLKACSGQKANGELKSSSLILLANIMQFLQRHEFGHVHDEWMSIVKPDLHPAVSAQLHEKFDVSEVEMENSKSVRSEMRVAVNSLLKDEGILVIPTVADPPPKLGGKEILSQDYQSRAFSLLSIASISGCCQVTVPLGFYDKYPVSVSLIARYGGDRFLLDTLKTMYTSLQEQADIAATSKTSRNVVSKEQSAEIAKEKGNQAYKDKQWQKAIGFYTEAIKLCGDNATYYSNRAQAYLELGSYLQAEADCTKAISLDKKSVKAYFRRGTAREMLGYYKEAIDDFKYALVLEPTNKRAASAAERLRKLFQ