AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95074

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95074.t1 MTR_4g134290 96.264 455 15 2 6 460 8 460 0.0 907
MS.gene95074.t1 MTR_3g110395 55.556 441 183 5 26 459 21 455 4.05e-178 509
MS.gene95074.t1 MTR_8g102190 55.329 441 183 6 26 458 20 454 5.02e-176 502
MS.gene95074.t1 MTR_6g090130 55.556 441 183 5 26 459 17 451 7.13e-175 500
MS.gene95074.t1 MTR_1g090300 54.072 442 190 5 26 460 87 522 1.75e-173 498
MS.gene95074.t1 MTR_2g100710 53.362 461 196 7 6 458 4 453 1.45e-170 488
MS.gene95074.t1 MTR_8g102190 54.722 360 149 6 26 377 20 373 5.01e-138 401
MS.gene95074.t1 MTR_8g102190 56.268 343 137 5 26 360 20 357 1.76e-136 398
MS.gene95074.t1 MTR_2g100710 54.821 363 147 6 6 360 4 357 7.59e-133 389
MS.gene95074.t1 MTR_8g069040 48.549 379 187 4 80 458 159 529 2.68e-120 362
MS.gene95074.t1 MTR_5g032570 45.646 379 198 4 80 458 163 533 1.50e-114 347
MS.gene95074.t1 MTR_2g100670 47.253 91 33 2 272 361 32 108 7.70e-17 82.8
MS.gene95074.t1 MTR_2g066850 51.351 74 35 1 170 243 389 461 3.57e-13 72.4
MS.gene95074.t1 MTR_2g025020 26.699 206 123 6 93 282 94 287 1.19e-12 70.5
MS.gene95074.t1 MTR_4g132700 24.365 197 139 4 93 282 98 291 1.98e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95074.t1 AT5G47810 75.510 441 90 5 26 457 7 438 0.0 676
MS.gene95074.t1 AT4G32840 55.682 440 179 6 26 458 21 451 2.05e-174 497
MS.gene95074.t1 AT4G26270 55.227 440 183 5 26 458 20 452 5.20e-174 497
MS.gene95074.t1 AT5G61580 54.091 440 189 5 26 458 71 504 1.99e-172 495
MS.gene95074.t1 AT5G56630 55.227 440 182 6 26 458 20 451 1.97e-171 491
MS.gene95074.t1 AT5G61580 54.091 440 188 6 26 458 71 503 1.38e-170 490
MS.gene95074.t1 AT4G29220 55.180 444 179 7 26 459 21 454 6.25e-169 484
MS.gene95074.t1 AT2G22480 47.906 382 185 5 80 458 167 537 7.54e-121 363
MS.gene95074.t1 AT4G04040 27.365 296 169 14 93 361 98 374 6.20e-13 71.2
MS.gene95074.t1 AT1G12000 26.633 199 132 4 93 282 96 289 6.74e-13 71.2

Find 115 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATTGAAGCCTTGATTT+TGG 0.165329 8.2:-540735 None:intergenic
GACCAACGACTGAAGGAAAA+TGG 0.215756 8.2:+541702 MS.gene95074:CDS
GAGAGCTTGTTGTTGGTCTT+TGG 0.225491 8.2:+540530 MS.gene95074:CDS
GGAATAATCGACAGATCTTT+TGG 0.247415 8.2:+541462 MS.gene95074:CDS
AGAGAAAACGAGGTCGTTTA+AGG 0.268173 8.2:-540383 None:intergenic
AGAGCTTGTTGTTGGTCTTT+GGG 0.275052 8.2:+540531 MS.gene95074:CDS
GGGTTTAGATCAAGTGGATT+TGG 0.287362 8.2:-540322 None:intergenic
CGTCAACAGGTGTACATTAT+AGG 0.310047 8.2:+541333 MS.gene95074:intron
TATGCATAATTGCCATTAAT+AGG 0.315023 8.2:-542032 None:intergenic
ACTCCAACATCTAAGAAAAC+TGG 0.333926 8.2:-541816 None:intergenic
GGATATACTGGATTTGTTGC+TGG 0.335094 8.2:+542008 MS.gene95074:CDS
TTTGTTGCTGGTCCTATTAA+TGG 0.347699 8.2:+542020 MS.gene95074:CDS
GATGTAGAAGTGTTGGAGTT+GGG 0.350430 8.2:-540359 None:intergenic
TTAATACAAAAGATCATAAT+TGG 0.352372 8.2:+542093 MS.gene95074:CDS
TGTCTAGCTCTTCAGAAAAT+CGG 0.360046 8.2:-542144 None:intergenic
GGTTTAGATCAAGTGGATTT+GGG 0.366705 8.2:-540321 None:intergenic
CTCTGCAATTCATGGTGTTA+TGG 0.369770 8.2:+541983 MS.gene95074:CDS
TTGGTCTTTGGGATCTCTAC+GGG 0.382473 8.2:+540542 MS.gene95074:CDS
CTTCAGAAAATCGGGTTGAT+TGG 0.401398 8.2:-542135 None:intergenic
ATGTAGAAGTGTTGGAGTTG+GGG 0.402048 8.2:-540358 None:intergenic
AGCTGAAGAAATGGTGGGAC+AGG 0.403942 8.2:+541853 MS.gene95074:CDS
AAACTGAATGTTGCAGTTGT+TGG 0.405610 8.2:+541411 MS.gene95074:CDS
GTTATTAGAGAGCTTGTTGT+TGG 0.416848 8.2:+540523 MS.gene95074:CDS
TGCCAGTGGTGAACAATACT+AGG 0.423482 8.2:-540643 None:intergenic
CTGTTCTTGGTATTATATCC+TGG 0.424765 8.2:-541758 None:intergenic
TTGGCACACTCTGCAATTCA+TGG 0.425156 8.2:+541975 MS.gene95074:CDS
TAATTTGTATTGTACACTCT+TGG 0.431844 8.2:+541956 MS.gene95074:CDS
ATAATTGTGTTACATCTTCA+AGG 0.436653 8.2:-540234 None:intergenic
GGTGTTATGGCTGGATATAC+TGG 0.436710 8.2:+541996 MS.gene95074:CDS
ATTTCAAACAGCAGTTGAAA+TGG 0.436991 8.2:+541485 MS.gene95074:CDS
CTCTTCTTCTACTCAACCGT+TGG 0.438008 8.2:+540609 MS.gene95074:CDS
ATTTGGGAAGGTCTTGAGGT+TGG 0.441059 8.2:-540305 None:intergenic
CTTGTAGTTGCCGAGGGTGC+TGG 0.445579 8.2:+541735 MS.gene95074:CDS
CCAAAATCAAGGCTTCAATC+AGG 0.445999 8.2:+540735 MS.gene95074:CDS
GTTGGTCTTTGGGATCTCTA+CGG 0.457538 8.2:+540541 MS.gene95074:CDS
TATAGGCCTTGTGAAGTTGA+TGG 0.459156 8.2:+541560 MS.gene95074:CDS
AAGGAAGAGCGAGATGAATC+TGG 0.459729 8.2:+541789 MS.gene95074:CDS
GTCTAGCTCTTCAGAAAATC+GGG 0.461245 8.2:-542143 None:intergenic
GAGAGTGCAGTTAACGGTAT+AGG 0.469199 8.2:+541543 MS.gene95074:CDS
CTGAAGAACAGTACCACCTT+TGG 0.476929 8.2:-540668 None:intergenic
CAAAAGATCATAATTGGTCA+TGG 0.484945 8.2:+542099 MS.gene95074:CDS
GCAATTCATGGTGTTATGGC+TGG 0.485776 8.2:+541987 MS.gene95074:CDS
GAAGACGCTGTTGCGAGGAC+CGG 0.491221 8.2:-540434 None:intergenic
AGTTGCCGAGGGTGCTGGCC+AGG 0.491874 8.2:+541740 MS.gene95074:CDS
ACCAACGACTGAAGGAAAAT+GGG 0.493978 8.2:+541703 MS.gene95074:CDS
AAGTTGATGGGTCGAAGCAC+AGG 0.498644 8.2:+541573 MS.gene95074:CDS
TGTAGAAGTGTTGGAGTTGG+GGG 0.499814 8.2:-540357 None:intergenic
TAAAGTCAGAGCTGAAGAAA+TGG 0.504227 8.2:+541844 MS.gene95074:CDS
ACATGCGGTGGTCTCTGTCC+TGG 0.504962 8.2:+540490 MS.gene95074:CDS
CTCCAACATCTAAGAAAACT+GGG 0.507207 8.2:-541815 None:intergenic
CTAATAACGGTGTTGAGTCC+AGG 0.509861 8.2:-540508 None:intergenic
TTAAGGTGATGTAGAAGTGT+TGG 0.514426 8.2:-540366 None:intergenic
GTGTACATTATAGGTGGAGA+TGG 0.515210 8.2:+541342 MS.gene95074:CDS
ATAGCAGCTCTTACATCAGA+AGG 0.525537 8.2:-540463 None:intergenic
AATTATCCGTAGCATTTGCA+TGG 0.527760 8.2:-541938 None:intergenic
ACCAAGAACAGAGTCACAAA+AGG 0.530216 8.2:+541770 MS.gene95074:CDS
TAAAACAGTGGATAATGATG+TGG 0.531127 8.2:+541440 MS.gene95074:CDS
GTGGATTTGGGAAGGTCTTG+AGG 0.531662 8.2:-540309 None:intergenic
TATATCCTGGCCAGCACCCT+CGG 0.533467 8.2:-541745 None:intergenic
AACAACAAGCTCTCTAATAA+CGG 0.535759 8.2:-540521 None:intergenic
CGCTTCGCTTATCGCAGCGC+CGG 0.540203 8.2:+540415 MS.gene95074:CDS
AAGTAAGGGTTTAGATCAAG+TGG 0.541976 8.2:-540328 None:intergenic
ACTGTTCTTCAGACTTCTAG+AGG 0.542115 8.2:+540679 MS.gene95074:CDS
GAAATGGCTCAGCAAGCAAT+CGG 0.545264 8.2:+541501 MS.gene95074:CDS
GAGGCCGAGAGTGCAGTTAA+CGG 0.545631 8.2:+541537 MS.gene95074:CDS
AGCAATCGGTGCTGCTCATG+TGG 0.547519 8.2:+541515 MS.gene95074:CDS
TGATGTAGAAGTGTTGGAGT+TGG 0.549753 8.2:-540360 None:intergenic
CAATTATGCATACATTCCAC+TGG 0.555213 8.2:+542043 MS.gene95074:CDS
GTTGATGCCATCCAAAATCA+AGG 0.555592 8.2:+540724 MS.gene95074:CDS
TGCAGTCCATGCAAATGCTA+CGG 0.557769 8.2:+541932 MS.gene95074:CDS
TGTTGGAATTCCTAAAACAG+TGG 0.558287 8.2:+541428 MS.gene95074:CDS
TAGATCAAGTGGATTTGGGA+AGG 0.564879 8.2:-540317 None:intergenic
TGCAGTGCTTGTAGTTGCCG+AGG 0.566674 8.2:+541728 MS.gene95074:CDS
ATAGGCCTTGTGAAGTTGAT+GGG 0.567702 8.2:+541561 MS.gene95074:CDS
AAAAGATCATAATTGGTCAT+GGG 0.567773 8.2:+542100 MS.gene95074:CDS
ACTGCACGAATCATGTATGT+AGG 0.577072 8.2:-541915 None:intergenic
ACCCTAGTATTGTTCACCAC+TGG 0.578946 8.2:+540641 MS.gene95074:CDS
TGGTGGGACAGGGACCATCC+TGG 0.579487 8.2:+541864 MS.gene95074:CDS
GTAGAAGTGTTGGAGTTGGG+GGG 0.580821 8.2:-540356 None:intergenic
GTTTCTTGACCAACGACTGA+AGG 0.583428 8.2:+541695 MS.gene95074:CDS
AGTCAGAGCTGAAGAAATGG+TGG 0.587927 8.2:+541847 MS.gene95074:CDS
GGTATCACCGCTGGTTACAG+AGG 0.588696 8.2:+540580 MS.gene95074:CDS
GCAGTGCTTGTAGTTGCCGA+GGG 0.592925 8.2:+541729 MS.gene95074:CDS
GGTGGAGATGGGACTATGCG+AGG 0.594506 8.2:+541354 MS.gene95074:CDS
TCTTGAGGTTGGAGATGTAG+TGG 0.595437 8.2:-540294 None:intergenic
GTGGAGATGGGACTATGCGA+GGG 0.599425 8.2:+541355 MS.gene95074:CDS
GTTCTTCAGACTTCTAGAGG+TGG 0.602049 8.2:+540682 MS.gene95074:CDS
TTTACTTGGAAGGAAAAGGA+GGG 0.603614 8.2:+541664 MS.gene95074:CDS
AATCGGTGCTGCTCATGTGG+AGG 0.603746 8.2:+541518 MS.gene95074:CDS
GCTGAAGAAATGGTGGGACA+GGG 0.604170 8.2:+541854 MS.gene95074:CDS
AAAACAGTGGATAATGATGT+GGG 0.607749 8.2:+541441 MS.gene95074:CDS
TGTACATTATAGGTGGAGAT+GGG 0.608105 8.2:+541343 MS.gene95074:CDS
CACCACTGGCATACCAAAGG+TGG 0.610272 8.2:+540655 MS.gene95074:CDS
TTGACTGTAAATAGCTCACC+AGG 0.616449 8.2:-541882 None:intergenic
CAACAGGTGTACATTATAGG+TGG 0.618654 8.2:+541336 MS.gene95074:intron
GCCAGTGGTGAACAATACTA+GGG 0.620862 8.2:-540642 None:intergenic
TTGAAGAAGACGCTGTTGCG+AGG 0.623813 8.2:-540439 None:intergenic
TATACCGTTAACTGCACTCT+CGG 0.626708 8.2:-541541 None:intergenic
TTCGACCCATCAACTTCACA+AGG 0.629286 8.2:-541566 None:intergenic
CTAGGGTTGAGCTGAACCAA+CGG 0.632100 8.2:-540625 None:intergenic
GAGATGTAGTGGTTGAGATG+AGG 0.632753 8.2:-540283 None:intergenic
CTGTAAATAGCTCACCAGGA+TGG 0.636294 8.2:-541878 None:intergenic
GCACATGCTACATCTTCCAG+TGG 0.639068 8.2:-542059 None:intergenic
GCTGCTATTGTAACATGCGG+TGG 0.641272 8.2:+540478 MS.gene95074:CDS
GTTCACCACTGGCATACCAA+AGG 0.645345 8.2:+540652 MS.gene95074:CDS
GTCAGAGCTGAAGAAATGGT+GGG 0.650633 8.2:+541848 MS.gene95074:CDS
TAGCAGCTCTTACATCAGAA+GGG 0.652881 8.2:-540462 None:intergenic
TGGTCTTTGGGATCTCTACG+GGG 0.653652 8.2:+540543 MS.gene95074:CDS
TTAGGCAGCAATCAACGTCA+CGG 0.665680 8.2:-541626 None:intergenic
TACCACCTTTGGTATGCCAG+TGG 0.676671 8.2:-540657 None:intergenic
TGGAGATGGGACTATGCGAG+GGG 0.690556 8.2:+541356 MS.gene95074:CDS
GTAAAAGCCTCTGTAACCAG+CGG 0.697919 8.2:-540587 None:intergenic
GCTGCGATAAGCGAAGCGTG+TGG 0.702419 8.2:-540410 None:intergenic
GTGTGGAGTGAGAGAAAACG+AGG 0.714528 8.2:-540393 None:intergenic
AGAGCTGCTATTGTAACATG+CGG 0.761108 8.2:+540475 MS.gene95074:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATACAAAAGATCATAAT+TGG + chr8.2:542093-542112 MS.gene95074:CDS 15.0%
!! AAGATTCAAGTAAATTGTAA+TGG - chr8.2:541063-541082 None:intergenic 20.0%
!!! AAACCAAAATCTAAAATGAA+AGG + chr8.2:540825-540844 MS.gene95074:intron 20.0%
!!! TATGATCTTTTGTATTAACT+GGG - chr8.2:542091-542110 None:intergenic 20.0%
!!! TTATGATCTTTTGTATTAAC+TGG - chr8.2:542092-542111 None:intergenic 20.0%
! AAAAGATCATAATTGGTCAT+GGG + chr8.2:542100-542119 MS.gene95074:CDS 25.0%
! ATAATTGTGTTACATCTTCA+AGG - chr8.2:540237-540256 None:intergenic 25.0%
! ATAGTTAAGGTGAAAATACA+AGG - chr8.2:541114-541133 None:intergenic 25.0%
! TAATTTGGAAGAAACACATA+AGG - chr8.2:541208-541227 None:intergenic 25.0%
! TATGCATAATTGCCATTAAT+AGG - chr8.2:542035-542054 None:intergenic 25.0%
!! AAGCCTTTCATTTTAGATTT+TGG - chr8.2:540831-540850 None:intergenic 25.0%
!! ATTTAACTTTATTGCTGCAA+AGG - chr8.2:540934-540953 None:intergenic 25.0%
!! GAATTTGAGATGAATAGTTA+AGG - chr8.2:541127-541146 None:intergenic 25.0%
!! TAATTTGTATTGTACACTCT+TGG + chr8.2:541956-541975 MS.gene95074:CDS 25.0%
!! TTTAACTTTATTGCTGCAAA+GGG - chr8.2:540933-540952 None:intergenic 25.0%
AAAACAGTGGATAATGATGT+GGG + chr8.2:541441-541460 MS.gene95074:CDS 30.0%
AACAACAAGCTCTCTAATAA+CGG - chr8.2:540524-540543 None:intergenic 30.0%
AATAAATAAGAGCTACTCGA+TGG + chr8.2:540995-541014 MS.gene95074:intron 30.0%
AATTCAACAATACTTCTGCT+TGG + chr8.2:540951-540970 MS.gene95074:intron 30.0%
ATTTCAAACAGCAGTTGAAA+TGG + chr8.2:541485-541504 MS.gene95074:CDS 30.0%
CAAAAGATCATAATTGGTCA+TGG + chr8.2:542099-542118 MS.gene95074:CDS 30.0%
GTTATAAGATGAGCAATGAA+AGG - chr8.2:540888-540907 None:intergenic 30.0%
TAAAACAGTGGATAATGATG+TGG + chr8.2:541440-541459 MS.gene95074:CDS 30.0%
TTAACTTTATTGCTGCAAAG+GGG - chr8.2:540932-540951 None:intergenic 30.0%
! AAAAATCTAGTTCAGGGATT+AGG - chr8.2:541647-541666 None:intergenic 30.0%
! ACATCATTATCCACTGTTTT+AGG - chr8.2:541441-541460 None:intergenic 30.0%
! CCAAGTAAAAATCTAGTTCA+GGG - chr8.2:541653-541672 None:intergenic 30.0%
! GAACTAGATTTTTACTTGGA+AGG + chr8.2:541654-541673 MS.gene95074:CDS 30.0%
! GATTTTTACTTGGAAGGAAA+AGG + chr8.2:541660-541679 MS.gene95074:CDS 30.0%
! TCCAAGTAAAAATCTAGTTC+AGG - chr8.2:541654-541673 None:intergenic 30.0%
!!! AGGAAGATCTTTTAGCTTAA+TGG - chr8.2:540266-540285 None:intergenic 30.0%
!!! GGAAGATCTTTTAGCTTAAT+GGG - chr8.2:540265-540284 None:intergenic 30.0%
AAACTGAATGTTGCAGTTGT+TGG + chr8.2:541411-541430 MS.gene95074:CDS 35.0%
AAGTAAGGGTTTAGATCAAG+TGG - chr8.2:540331-540350 None:intergenic 35.0%
AATTATCCGTAGCATTTGCA+TGG - chr8.2:541941-541960 None:intergenic 35.0%
ACTCCAACATCTAAGAAAAC+TGG - chr8.2:541819-541838 None:intergenic 35.0%
CAATTATGCATACATTCCAC+TGG + chr8.2:542043-542062 MS.gene95074:CDS 35.0%
CCAGCTAAAGTAAACTTTAC+AGG - chr8.2:541158-541177 None:intergenic 35.0%
CCTGTAAAGTTTACTTTAGC+TGG + chr8.2:541155-541174 MS.gene95074:intron 35.0%
CTCCAACATCTAAGAAAACT+GGG - chr8.2:541818-541837 None:intergenic 35.0%
CTGTTCTTGGTATTATATCC+TGG - chr8.2:541761-541780 None:intergenic 35.0%
GTGTTTCTTCCAAATTAAGC+TGG + chr8.2:541211-541230 MS.gene95074:intron 35.0%
TAAAGTCAGAGCTGAAGAAA+TGG + chr8.2:541844-541863 MS.gene95074:CDS 35.0%
TGTACATTATAGGTGGAGAT+GGG + chr8.2:541343-541362 MS.gene95074:CDS 35.0%
TGTTGGAATTCCTAAAACAG+TGG + chr8.2:541428-541447 MS.gene95074:CDS 35.0%
TTGGAAGAAACACATAAGGA+AGG - chr8.2:541204-541223 None:intergenic 35.0%
TTTACTTGGAAGGAAAAGGA+GGG + chr8.2:541664-541683 MS.gene95074:CDS 35.0%
TTTGTTGCTGGTCCTATTAA+TGG + chr8.2:542020-542039 MS.gene95074:CDS 35.0%
! AACCCAGTTTTCTTAGATGT+TGG + chr8.2:541813-541832 MS.gene95074:CDS 35.0%
! ATTGAAGCCTTGATTTTGGA+TGG - chr8.2:540734-540753 None:intergenic 35.0%
! ATTTTCCAGCCAGCTTAATT+TGG - chr8.2:541223-541242 None:intergenic 35.0%
! CCCTGAACTAGATTTTTACT+TGG + chr8.2:541650-541669 MS.gene95074:CDS 35.0%
! GGAATAATCGACAGATCTTT+TGG + chr8.2:541462-541481 MS.gene95074:CDS 35.0%
! GGTTTAGATCAAGTGGATTT+GGG - chr8.2:540324-540343 None:intergenic 35.0%
! TTAAGGTGATGTAGAAGTGT+TGG - chr8.2:540369-540388 None:intergenic 35.0%
! TTTTACTTGGAAGGAAAAGG+AGG + chr8.2:541663-541682 MS.gene95074:CDS 35.0%
! TTTTCTTAGATGTTGGAGTG+TGG + chr8.2:541820-541839 MS.gene95074:CDS 35.0%
!! GTTATTAGAGAGCTTGTTGT+TGG + chr8.2:540523-540542 MS.gene95074:CDS 35.0%
AAGAGCTACTCGATGGAATA+TGG + chr8.2:541002-541021 MS.gene95074:intron 40.0%
ACCAACGACTGAAGGAAAAT+GGG + chr8.2:541703-541722 MS.gene95074:CDS 40.0%
ACCAAGAACAGAGTCACAAA+AGG + chr8.2:541770-541789 MS.gene95074:CDS 40.0%
ACTGCACGAATCATGTATGT+AGG - chr8.2:541918-541937 None:intergenic 40.0%
ACTGTTCTTCAGACTTCTAG+AGG + chr8.2:540679-540698 MS.gene95074:CDS 40.0%
AGAGAAAACGAGGTCGTTTA+AGG - chr8.2:540386-540405 None:intergenic 40.0%
AGAGCTGCTATTGTAACATG+CGG + chr8.2:540475-540494 MS.gene95074:CDS 40.0%
ATAGCAGCTCTTACATCAGA+AGG - chr8.2:540466-540485 None:intergenic 40.0%
CAACAGGTGTACATTATAGG+TGG + chr8.2:541336-541355 MS.gene95074:intron 40.0%
CCAAAATCAAGGCTTCAATC+AGG + chr8.2:540735-540754 MS.gene95074:CDS 40.0%
CGTCAACAGGTGTACATTAT+AGG + chr8.2:541333-541352 MS.gene95074:intron 40.0%
GGAGTGATATCTGAATTGCA+CGG - chr8.2:541183-541202 None:intergenic 40.0%
GTGTACATTATAGGTGGAGA+TGG + chr8.2:541342-541361 MS.gene95074:CDS 40.0%
GTTGATGCCATCCAAAATCA+AGG + chr8.2:540724-540743 MS.gene95074:CDS 40.0%
TAGCAGCTCTTACATCAGAA+GGG - chr8.2:540465-540484 None:intergenic 40.0%
TATACCGTTAACTGCACTCT+CGG - chr8.2:541544-541563 None:intergenic 40.0%
TTCTTCCAAATTAAGCTGGC+TGG + chr8.2:541215-541234 MS.gene95074:intron 40.0%
TTGACTGTAAATAGCTCACC+AGG - chr8.2:541885-541904 None:intergenic 40.0%
! ATAGGCCTTGTGAAGTTGAT+GGG + chr8.2:541561-541580 MS.gene95074:CDS 40.0%
! CCTGATTGAAGCCTTGATTT+TGG - chr8.2:540738-540757 None:intergenic 40.0%
! CTCTGCAATTCATGGTGTTA+TGG + chr8.2:541983-542002 MS.gene95074:CDS 40.0%
! GGGTTTAGATCAAGTGGATT+TGG - chr8.2:540325-540344 None:intergenic 40.0%
! TAGATCAAGTGGATTTGGGA+AGG - chr8.2:540320-540339 None:intergenic 40.0%
! TATAGGCCTTGTGAAGTTGA+TGG + chr8.2:541560-541579 MS.gene95074:CDS 40.0%
! TCCTTTTGTGACTCTGTTCT+TGG - chr8.2:541774-541793 None:intergenic 40.0%
!! AGAGCTTGTTGTTGGTCTTT+GGG + chr8.2:540531-540550 MS.gene95074:CDS 40.0%
!! ATGTAGAAGTGTTGGAGTTG+GGG - chr8.2:540361-540380 None:intergenic 40.0%
!! CTTCAGAAAATCGGGTTGAT+TGG - chr8.2:542138-542157 None:intergenic 40.0%
!! GATGTAGAAGTGTTGGAGTT+GGG - chr8.2:540362-540381 None:intergenic 40.0%
!! GGATATACTGGATTTGTTGC+TGG + chr8.2:542008-542027 MS.gene95074:CDS 40.0%
!! TGATGTAGAAGTGTTGGAGT+TGG - chr8.2:540363-540382 None:intergenic 40.0%
AAGGAAGAGCGAGATGAATC+TGG + chr8.2:541789-541808 MS.gene95074:CDS 45.0%
ACCCTAGTATTGTTCACCAC+TGG + chr8.2:540641-540660 MS.gene95074:CDS 45.0%
AGTCAGAGCTGAAGAAATGG+TGG + chr8.2:541847-541866 MS.gene95074:CDS 45.0%
ATCATCCTGTTCTCGTCAAC+AGG + chr8.2:541320-541339 MS.gene95074:intron 45.0%
ATTTGGGAAGGTCTTGAGGT+TGG - chr8.2:540308-540327 None:intergenic 45.0%
CTCTTCTTCTACTCAACCGT+TGG + chr8.2:540609-540628 MS.gene95074:CDS 45.0%
CTGAAGAACAGTACCACCTT+TGG - chr8.2:540671-540690 None:intergenic 45.0%
CTGTAAATAGCTCACCAGGA+TGG - chr8.2:541881-541900 None:intergenic 45.0%
GAAATGGCTCAGCAAGCAAT+CGG + chr8.2:541501-541520 MS.gene95074:CDS 45.0%
GACCAACGACTGAAGGAAAA+TGG + chr8.2:541702-541721 MS.gene95074:CDS 45.0%
GAGATGTAGTGGTTGAGATG+AGG - chr8.2:540286-540305 None:intergenic 45.0%
GCCAGTGGTGAACAATACTA+GGG - chr8.2:540645-540664 None:intergenic 45.0%
GCGAAACTCACAACACAAAC+TGG + chr8.2:540773-540792 MS.gene95074:intron 45.0%
GTAAAAGCCTCTGTAACCAG+CGG - chr8.2:540590-540609 None:intergenic 45.0%
GTCAGAGCTGAAGAAATGGT+GGG + chr8.2:541848-541867 MS.gene95074:CDS 45.0%
GTTTCTTGACCAACGACTGA+AGG + chr8.2:541695-541714 MS.gene95074:CDS 45.0%
TCTTGAGGTTGGAGATGTAG+TGG - chr8.2:540297-540316 None:intergenic 45.0%
TGCAGTCCATGCAAATGCTA+CGG + chr8.2:541932-541951 MS.gene95074:CDS 45.0%
TGCCAGTGGTGAACAATACT+AGG - chr8.2:540646-540665 None:intergenic 45.0%
TTAGGCAGCAATCAACGTCA+CGG - chr8.2:541629-541648 None:intergenic 45.0%
TTCGACCCATCAACTTCACA+AGG - chr8.2:541569-541588 None:intergenic 45.0%
TTGGCACACTCTGCAATTCA+TGG + chr8.2:541975-541994 MS.gene95074:CDS 45.0%
! CTAATAACGGTGTTGAGTCC+AGG - chr8.2:540511-540530 None:intergenic 45.0%
! GAGAGTGCAGTTAACGGTAT+AGG + chr8.2:541543-541562 MS.gene95074:CDS 45.0%
! GGTGTTATGGCTGGATATAC+TGG + chr8.2:541996-542015 MS.gene95074:CDS 45.0%
! GTTCTTCAGACTTCTAGAGG+TGG + chr8.2:540682-540701 MS.gene95074:CDS 45.0%
! TACGGGGTTCGACAGATTTT+TGG + chr8.2:540559-540578 MS.gene95074:CDS 45.0%
!! GAGAGCTTGTTGTTGGTCTT+TGG + chr8.2:540530-540549 MS.gene95074:CDS 45.0%
!! GCAATTCATGGTGTTATGGC+TGG + chr8.2:541987-542006 MS.gene95074:CDS 45.0%
!! GTTGGTCTTTGGGATCTCTA+CGG + chr8.2:540541-540560 MS.gene95074:CDS 45.0%
!! TGTAGAAGTGTTGGAGTTGG+GGG - chr8.2:540360-540379 None:intergenic 45.0%
!! TTGGTCTTTGGGATCTCTAC+GGG + chr8.2:540542-540561 MS.gene95074:CDS 45.0%
!!! CAGATTTTTGGTATCACCGC+TGG + chr8.2:540571-540590 MS.gene95074:CDS 45.0%
ATTGCTGCAAAGGGGAAGAG+AGG - chr8.2:540924-540943 None:intergenic 50.0%
CTAGGGTTGAGCTGAACCAA+CGG - chr8.2:540628-540647 None:intergenic 50.0%
GCACATGCTACATCTTCCAG+TGG - chr8.2:542062-542081 None:intergenic 50.0%
GCTGCTATTGTAACATGCGG+TGG + chr8.2:540478-540497 MS.gene95074:CDS 50.0%
GGCATCAGCAAAGACTCACT+CGG - chr8.2:540867-540886 None:intergenic 50.0%
GTACACCTGTTGACGAGAAC+AGG - chr8.2:541328-541347 None:intergenic 50.0%
GTGGATTTGGGAAGGTCTTG+AGG - chr8.2:540312-540331 None:intergenic 50.0%
GTGTGGAGTGAGAGAAAACG+AGG - chr8.2:540396-540415 None:intergenic 50.0%
GTTCACCACTGGCATACCAA+AGG + chr8.2:540652-540671 MS.gene95074:CDS 50.0%
TACCACCTTTGGTATGCCAG+TGG - chr8.2:540660-540679 None:intergenic 50.0%
TTGAAGAAGACGCTGTTGCG+AGG - chr8.2:540442-540461 None:intergenic 50.0%
! AGCTGAAGAAATGGTGGGAC+AGG + chr8.2:541853-541872 MS.gene95074:CDS 50.0%
! GCCCATTTTCCTTCAGTCGT+TGG - chr8.2:541707-541726 None:intergenic 50.0%
! GCTGAAGAAATGGTGGGACA+GGG + chr8.2:541854-541873 MS.gene95074:CDS 50.0%
!! AAGTTGATGGGTCGAAGCAC+AGG + chr8.2:541573-541592 MS.gene95074:CDS 50.0%
!! GTAGAAGTGTTGGAGTTGGG+GGG - chr8.2:540359-540378 None:intergenic 50.0%
!! TGGTCTTTGGGATCTCTACG+GGG + chr8.2:540543-540562 MS.gene95074:CDS 50.0%
CACCACTGGCATACCAAAGG+TGG + chr8.2:540655-540674 MS.gene95074:CDS 55.0%
GCAGTGCTTGTAGTTGCCGA+GGG + chr8.2:541729-541748 MS.gene95074:CDS 55.0%
GTGGAGATGGGACTATGCGA+GGG + chr8.2:541355-541374 MS.gene95074:CDS 55.0%
TATATCCTGGCCAGCACCCT+CGG - chr8.2:541748-541767 None:intergenic 55.0%
TGCAGTGCTTGTAGTTGCCG+AGG + chr8.2:541728-541747 MS.gene95074:CDS 55.0%
TGGAGATGGGACTATGCGAG+GGG + chr8.2:541356-541375 MS.gene95074:CDS 55.0%
! AGTTGGGGGGTGTGGAAGTA+AGG - chr8.2:540346-540365 None:intergenic 55.0%
! GAGGCCGAGAGTGCAGTTAA+CGG + chr8.2:541537-541556 MS.gene95074:CDS 55.0%
! GGTATCACCGCTGGTTACAG+AGG + chr8.2:540580-540599 MS.gene95074:CDS 55.0%
! GTTGGGGGGTGTGGAAGTAA+GGG - chr8.2:540345-540364 None:intergenic 55.0%
!! AATCGGTGCTGCTCATGTGG+AGG + chr8.2:541518-541537 MS.gene95074:CDS 55.0%
!! AGCAATCGGTGCTGCTCATG+TGG + chr8.2:541515-541534 MS.gene95074:CDS 55.0%
ACATGCGGTGGTCTCTGTCC+TGG + chr8.2:540490-540509 MS.gene95074:CDS 60.0%
GAAGACGCTGTTGCGAGGAC+CGG - chr8.2:540437-540456 None:intergenic 60.0%
GCTGCAAAGGGGAAGAGAGG+TGG - chr8.2:540921-540940 None:intergenic 60.0%
GCTGCGATAAGCGAAGCGTG+TGG - chr8.2:540413-540432 None:intergenic 60.0%
GGTGGAGATGGGACTATGCG+AGG + chr8.2:541354-541373 MS.gene95074:CDS 60.0%
! AGTGTTGGAGTTGGGGGGTG+TGG - chr8.2:540354-540373 None:intergenic 60.0%
! CTTGTAGTTGCCGAGGGTGC+TGG + chr8.2:541735-541754 MS.gene95074:CDS 60.0%
CGCTTCGCTTATCGCAGCGC+CGG + chr8.2:540415-540434 MS.gene95074:CDS 65.0%
TGGTGGGACAGGGACCATCC+TGG + chr8.2:541864-541883 MS.gene95074:CDS 65.0%
!! AGTTGCCGAGGGTGCTGGCC+AGG + chr8.2:541740-541759 MS.gene95074:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 540196 542163 540196 ID=MS.gene95074
chr8.2 mRNA 540196 542163 540196 ID=MS.gene95074.t1;Parent=MS.gene95074
chr8.2 exon 540196 540756 540196 ID=MS.gene95074.t1.exon1;Parent=MS.gene95074.t1
chr8.2 CDS 540196 540756 540196 ID=cds.MS.gene95074.t1;Parent=MS.gene95074.t1
chr8.2 exon 541342 542163 541342 ID=MS.gene95074.t1.exon2;Parent=MS.gene95074.t1
chr8.2 CDS 541342 542163 541342 ID=cds.MS.gene95074.t1;Parent=MS.gene95074.t1
Gene Sequence

>MS.gene95074

ATGGAGGATGATTTTTCTTCTTCTTCTTCTTTAGAACTCCTTGAAGATGTAACACAATTATTTCAACCCATTAAGCTAAAAGATCTTCCTCATCTCAACCACTACATCTCCAACCTCAAGACCTTCCCAAATCCACTTGATCTAAACCCTTACTTCCACACCCCCCAACTCCAACACTTCTACATCACCTTAAACGACCTCGTTTTCTCTCACTCCACACGCTTCGCTTATCGCAGCGCCGGTCCTCGCAACAGCGTCTTCTTCAACCCTTCTGATGTAAGAGCTGCTATTGTAACATGCGGTGGTCTCTGTCCTGGACTCAACACCGTTATTAGAGAGCTTGTTGTTGGTCTTTGGGATCTCTACGGGGTTCGACAGATTTTTGGTATCACCGCTGGTTACAGAGGCTTTTACTCTTCTTCTACTCAACCGTTGGTTCAGCTCAACCCTAGTATTGTTCACCACTGGCATACCAAAGGTGGTACTGTTCTTCAGACTTCTAGAGGTGGTTTTGATTTATGCAAGATCGTTGATGCCATCCAAAATCAAGGCTTCAATCAGGTTTTGTTTTGTTAATGCGAAACTCACAACACAAACTGGAATTAAGTTTTACTAGAATTAAATAAAAGAAACCAAAATCTAAAATGAAAGGCTTTTTAATCTATCAACCGAGTGAGTCTTTGCTGATGCCTTTCATTGCTCATCTTATAACTGACTGAGTGCCACCTCTCTTCCCCTTTGCAGCAATAAAGTTAAATTCAACAATACTTCTGCTTGGAAATGCATTGATATCACACATAATAAATAAGAGCTACTCGATGGAATATGGATCACTGTGTGTGATGTATAATAACTTTGACGTCACCATTACAATTTACTTGAATCTTATGCTTTTAGAATCAATTTGTTTATTATCCTTGTATTTTCACCTTAACTATTCATCTCAAATTCAGTCTTGTCCTGTAAAGTTTACTTTAGCTGGAACCGTGCAATTCAGATATCACTCCTTCCTTATGTGTTTCTTCCAAATTAAGCTGGCTGGAAAATATATACAAATAAATATTGAAAAAAAAAAAAAAAGAAAAGAGAAACTATTGCATTCATACATACTTATTAATGCACTTATCATCCTGTTCTCGTCAACAGGTGTACATTATAGGTGGAGATGGGACTATGCGAGGGGCTGTGAAGATATTCAATGAAATAAAGCAACGTAAACTGAATGTTGCAGTTGTTGGAATTCCTAAAACAGTGGATAATGATGTGGGAATAATCGACAGATCTTTTGGATTTCAAACAGCAGTTGAAATGGCTCAGCAAGCAATCGGTGCTGCTCATGTGGAGGCCGAGAGTGCAGTTAACGGTATAGGCCTTGTGAAGTTGATGGGTCGAAGCACAGGTCACATCGTTCTTCATGCAACACTCAGCAGCCGTGACGTTGATTGCTGCCTAATCCCTGAACTAGATTTTTACTTGGAAGGAAAAGGAGGGCTCTTTGAGTTTCTTGACCAACGACTGAAGGAAAATGGGCATGCAGTGCTTGTAGTTGCCGAGGGTGCTGGCCAGGATATAATACCAAGAACAGAGTCACAAAAGGAAGAGCGAGATGAATCTGGTAACCCAGTTTTCTTAGATGTTGGAGTGTGGTTAAAGTCAGAGCTGAAGAAATGGTGGGACAGGGACCATCCTGGTGAGCTATTTACAGTCAAGTATATAGATCCTACATACATGATTCGTGCAGTCCATGCAAATGCTACGGATAATTTGTATTGTACACTCTTGGCACACTCTGCAATTCATGGTGTTATGGCTGGATATACTGGATTTGTTGCTGGTCCTATTAATGGCAATTATGCATACATTCCACTGGAAGATGTAGCATGTGCAAAAAACCCAGTTAATACAAAAGATCATAATTGGTCATGGGTGAGATCTGTCACCAATCAACCCGATTTTCTGAAGAGCTAG
Protein sequence

>MS.gene95074.t1

MEDDFSSSSSLELLEDVTQLFQPIKLKDLPHLNHYISNLKTFPNPLDLNPYFHTPQLQHFYITLNDLVFSHSTRFAYRSAGPRNSVFFNPSDVRAAIVTCGGLCPGLNTVIRELVVGLWDLYGVRQIFGITAGYRGFYSSSTQPLVQLNPSIVHHWHTKGGTVLQTSRGGFDLCKIVDAIQNQGFNQVYIIGGDGTMRGAVKIFNEIKQRKLNVAVVGIPKTVDNDVGIIDRSFGFQTAVEMAQQAIGAAHVEAESAVNGIGLVKLMGRSTGHIVLHATLSSRDVDCCLIPELDFYLEGKGGLFEFLDQRLKENGHAVLVVAEGAGQDIIPRTESQKEERDESGNPVFLDVGVWLKSELKKWWDRDHPGELFTVKYIDPTYMIRAVHANATDNLYCTLLAHSAIHGVMAGYTGFVAGPINGNYAYIPLEDVACAKNPVNTKDHNWSWVRSVTNQPDFLKS