Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95690.t1 | AET03441.2 | 99 | 314 | 3 | 0 | 1 | 314 | 1 | 314 | 1.90E-174 | 621.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95690.t1 | Q8LGI3 | 70.0 | 313 | 88 | 2 | 1 | 313 | 1 | 307 | 1.1e-122 | 441.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95690.t1 | G7L724 | 99.0 | 314 | 3 | 0 | 1 | 314 | 1 | 314 | 1.4e-174 | 621.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene95690 | MS.gene005307 | PPI |
| MS.gene95690 | MS.gene44181 | PPI |
| MS.gene95690 | MS.gene40797 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95690.t1 | MTR_8g070640 | 99.045 | 314 | 3 | 0 | 1 | 314 | 1 | 314 | 0.0 | 644 |
| MS.gene95690.t1 | MTR_1g045065 | 38.111 | 307 | 185 | 4 | 12 | 318 | 11 | 312 | 6.10e-74 | 230 |
| MS.gene95690.t1 | MTR_1g045065 | 37.662 | 231 | 142 | 2 | 12 | 242 | 11 | 239 | 7.31e-51 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95690.t1 | AT1G30825 | 69.745 | 314 | 89 | 2 | 1 | 314 | 1 | 308 | 7.45e-164 | 459 |
| MS.gene95690.t1 | AT2G33385 | 35.974 | 303 | 191 | 3 | 1 | 302 | 1 | 301 | 8.72e-71 | 222 |
| MS.gene95690.t1 | AT2G33385 | 35.974 | 303 | 191 | 3 | 1 | 302 | 1 | 301 | 4.66e-70 | 223 |
| MS.gene95690.t1 | AT2G33385 | 36.644 | 292 | 182 | 3 | 1 | 291 | 1 | 290 | 1.35e-69 | 219 |
| MS.gene95690.t1 | AT2G33385 | 35.314 | 303 | 184 | 4 | 1 | 302 | 1 | 292 | 5.22e-66 | 212 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATACTGTCCGATCCAGTTT+TGG | 0.217961 | 8.4:-32758693 | None:intergenic |
| TTCTGTTTCAGTATTGAAAA+AGG | 0.272592 | 8.4:+32754109 | MS.gene95690:intron |
| TAGAAATGTTGCTCCGGATA+TGG | 0.274270 | 8.4:+32756553 | MS.gene95690:CDS |
| TCTTGCAACTTCATTCTTGC+AGG | 0.282801 | 8.4:+32758257 | MS.gene95690:CDS |
| TTTGTATGTGGACTTCCTTT+TGG | 0.298544 | 8.4:+32754983 | MS.gene95690:CDS |
| ACAGATAGCAAGAAAATATT+AGG | 0.300457 | 8.4:-32754929 | None:intergenic |
| TCGTGTGGAGTCTTTGATTC+AGG | 0.327828 | 8.4:+32759920 | MS.gene95690:CDS |
| ATAATACCCCTCCTTGTTCA+TGG | 0.331553 | 8.4:+32758481 | MS.gene95690:CDS |
| AATGCAGGATTTGTATCATT+TGG | 0.336445 | 8.4:+32758558 | MS.gene95690:CDS |
| TGCAGCTTTGATTGCTTCAA+TGG | 0.339557 | 8.4:-32755008 | None:intergenic |
| TTCTTTGAACTCTCAGGATC+TGG | 0.345649 | 8.4:-32767141 | None:intergenic |
| TCATGTTGAAGGCCCAAAAC+TGG | 0.365625 | 8.4:+32758680 | MS.gene95690:CDS |
| AGAGCAAAGACAAGCACTTT+TGG | 0.382841 | 8.4:+32756460 | MS.gene95690:intron |
| GTAGATACTCCTTTCAGTTC+CGG | 0.383827 | 8.4:-32758519 | None:intergenic |
| CTATGTTAGTTATCATGTTA+AGG | 0.387046 | 8.4:+32758737 | MS.gene95690:CDS |
| CCGAAGGATTTATGCATACT+AGG | 0.392538 | 8.4:+32759889 | MS.gene95690:CDS |
| ATCTTCCCTCGTCATGTTGA+AGG | 0.393552 | 8.4:+32758669 | MS.gene95690:CDS |
| CAAAACTGGATCGGACAGTA+TGG | 0.412013 | 8.4:+32758694 | MS.gene95690:CDS |
| CGATGTCAGATATCACATTC+AGG | 0.427268 | 8.4:+32754165 | MS.gene95690:CDS |
| TCAAAGGTTCCTGCAAATCA+AGG | 0.450216 | 8.4:+32755100 | MS.gene95690:CDS |
| TGGTAAAAGTAGCATCAGTA+AGG | 0.458645 | 8.4:+32756480 | MS.gene95690:CDS |
| ATCGTTGCCTACTCCATCTT+CGG | 0.458816 | 8.4:+32754952 | MS.gene95690:CDS |
| GAAGCAATCAAAGCTGCATA+TGG | 0.481422 | 8.4:+32755013 | MS.gene95690:CDS |
| GTCGAAGCCAGGCGTACAGC+TGG | 0.483399 | 8.4:+32758453 | MS.gene95690:CDS |
| ATTAAGTCCAGCTGTACGCC+TGG | 0.485840 | 8.4:-32758460 | None:intergenic |
| ACTCCTTTCAGTTCCGGTGG+TGG | 0.485924 | 8.4:-32758513 | None:intergenic |
| TCGTCCTTGGTGCACCACTA+AGG | 0.490974 | 8.4:+32756507 | MS.gene95690:CDS |
| CCTAGTATGCATAAATCCTT+CGG | 0.491877 | 8.4:-32759889 | None:intergenic |
| GCGTTGAACTCGATCACCAC+TGG | 0.492167 | 8.4:+32754131 | MS.gene95690:CDS |
| TTTAGGAATTTGTCGAAGCC+AGG | 0.492797 | 8.4:+32758442 | MS.gene95690:intron |
| TGCAAATTCTTGATCCTCCA+AGG | 0.496434 | 8.4:+32755044 | MS.gene95690:CDS |
| TGCTTCTAGAAATGTTGCTC+CGG | 0.496839 | 8.4:+32756547 | MS.gene95690:CDS |
| CGTCCTTGGTGCACCACTAA+GGG | 0.507682 | 8.4:+32756508 | MS.gene95690:CDS |
| GCAACAAGTGGATCCATATC+CGG | 0.514116 | 8.4:-32756566 | None:intergenic |
| TCAGTAAGGGAGGTCGTCCT+TGG | 0.514232 | 8.4:+32756494 | MS.gene95690:CDS |
| CTGAAGCAAGAATCTAGAAT+GGG | 0.518291 | 8.4:-32753978 | None:intergenic |
| GCAAGAATCTAGAATGGGAC+TGG | 0.526581 | 8.4:-32753973 | None:intergenic |
| TCTGAAGCAAGAATCTAGAA+TGG | 0.526800 | 8.4:-32753979 | None:intergenic |
| ATTGCTTCAATGGCTCCAAA+AGG | 0.535755 | 8.4:-32754998 | None:intergenic |
| ATCGAATCATTGAATCTCAT+AGG | 0.537796 | 8.4:-32758225 | None:intergenic |
| TACTAGGATGCGTCGTCGTG+TGG | 0.540037 | 8.4:+32759905 | MS.gene95690:CDS |
| GTGAGGTTAAAGCCATCCCT+TGG | 0.545057 | 8.4:-32755061 | None:intergenic |
| ATTGAAGATAAATTTCTCAA+AGG | 0.549287 | 8.4:+32755084 | MS.gene95690:CDS |
| CTTAACATGATAACTAACAT+AGG | 0.549462 | 8.4:-32758736 | None:intergenic |
| TGCTCTTGTTCATCGACCAA+AGG | 0.549781 | 8.4:+32756586 | MS.gene95690:CDS |
| TATTTGTAACAGTGCTCCGA+AGG | 0.554094 | 8.4:+32759873 | MS.gene95690:intron |
| GATGCATTATCTGCAAATGC+AGG | 0.555094 | 8.4:+32758543 | MS.gene95690:CDS |
| ATTCTTGATCCTCCAAGGGA+TGG | 0.567110 | 8.4:+32755049 | MS.gene95690:CDS |
| CAGCAGATCCTTCCAACGAC+TGG | 0.570937 | 8.4:+32767173 | MS.gene95690:CDS |
| AGAAATTTATCTTCAATGTG+AGG | 0.573141 | 8.4:-32755078 | None:intergenic |
| AGGTTAAAGCCATCCCTTGG+AGG | 0.592449 | 8.4:-32755058 | None:intergenic |
| ATGGTCTCATACTCCACCAC+CGG | 0.595235 | 8.4:+32758500 | MS.gene95690:CDS |
| CGTTGAACTCGATCACCACT+GGG | 0.595892 | 8.4:+32754132 | MS.gene95690:CDS |
| ATAACGAACCAGTCGTTGGA+AGG | 0.600616 | 8.4:-32767181 | None:intergenic |
| GCACATAACGAACCAGTCGT+TGG | 0.604353 | 8.4:-32767185 | None:intergenic |
| TTGAAGGCCCAAAACTGGAT+CGG | 0.604670 | 8.4:+32758685 | MS.gene95690:CDS |
| GGTAAAAGTAGCATCAGTAA+GGG | 0.605688 | 8.4:+32756481 | MS.gene95690:CDS |
| TATGAGACCATGAACAAGGA+GGG | 0.608289 | 8.4:-32758488 | None:intergenic |
| GCAAATTCTTGATCCTCCAA+GGG | 0.614206 | 8.4:+32755045 | MS.gene95690:CDS |
| GAGCAACATTTCTAGAAGCA+AGG | 0.614317 | 8.4:-32756544 | None:intergenic |
| ACTCCACCACCGGAACTGAA+AGG | 0.622847 | 8.4:+32758510 | MS.gene95690:CDS |
| GATACTCCTTTCAGTTCCGG+TGG | 0.623232 | 8.4:-32758516 | None:intergenic |
| ATAGTTTCCGAAGATGGAGT+AGG | 0.625652 | 8.4:-32754959 | None:intergenic |
| TTTGGGCCTTCAACATGACG+AGG | 0.628818 | 8.4:-32758675 | None:intergenic |
| CGATGAACAAGAGCAACAAG+TGG | 0.645702 | 8.4:-32756578 | None:intergenic |
| GGAGTATGAGACCATGAACA+AGG | 0.647126 | 8.4:-32758492 | None:intergenic |
| AGAATCTAGAATGGGACTGG+AGG | 0.653361 | 8.4:-32753970 | None:intergenic |
| AAAAGTAGCATCAGTAAGGG+AGG | 0.660193 | 8.4:+32756484 | MS.gene95690:CDS |
| TTGGGCCTTCAACATGACGA+GGG | 0.678323 | 8.4:-32758674 | None:intergenic |
| ATGAGACCATGAACAAGGAG+GGG | 0.678416 | 8.4:-32758487 | None:intergenic |
| CATCGTCAAATTCAACCCAG+TGG | 0.726847 | 8.4:-32754147 | None:intergenic |
| GTATGAGACCATGAACAAGG+AGG | 0.729871 | 8.4:-32758489 | None:intergenic |
| TTACCCTTAGTGGTGCACCA+AGG | 0.750127 | 8.4:-32756511 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 32753962 | 32767882 | 32753962 | ID=MS.gene95690 |
| chr8.4 | mRNA | 32753962 | 32767882 | 32753962 | ID=MS.gene95690.t1;Parent=MS.gene95690 |
| chr8.4 | exon | 32753962 | 32754023 | 32753962 | ID=MS.gene95690.t1.exon1;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32753962 | 32754023 | 32753962 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32754120 | 32754186 | 32754120 | ID=MS.gene95690.t1.exon2;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32754120 | 32754186 | 32754120 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32754914 | 32755121 | 32754914 | ID=MS.gene95690.t1.exon3;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32754914 | 32755121 | 32754914 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32756462 | 32756631 | 32756462 | ID=MS.gene95690.t1.exon4;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32756462 | 32756631 | 32756462 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32758201 | 32758278 | 32758201 | ID=MS.gene95690.t1.exon5;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32758201 | 32758278 | 32758201 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32758447 | 32758579 | 32758447 | ID=MS.gene95690.t1.exon6;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32758447 | 32758579 | 32758447 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32758667 | 32758758 | 32758667 | ID=MS.gene95690.t1.exon7;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32758667 | 32758758 | 32758667 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32759885 | 32759941 | 32759885 | ID=MS.gene95690.t1.exon8;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32759885 | 32759941 | 32759885 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32767123 | 32767194 | 32767123 | ID=MS.gene95690.t1.exon9;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32767123 | 32767194 | 32767123 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| chr8.4 | exon | 32767853 | 32767882 | 32767853 | ID=MS.gene95690.t1.exon10;Parent=MS.gene95690.t1 |
| chr8.4 | CDS | 32767853 | 32767882 | 32767853 | ID=cds.MS.gene95690.t1;Parent=MS.gene95690.t1 |
| Gene Sequence |
| Protein sequence |
