Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95747.t1 | XP_013455508.1 | 97.4 | 114 | 3 | 0 | 1 | 114 | 1 | 114 | 3.00E-56 | 228 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95747.t1 | Q9C6T5 | 55.6 | 63 | 20 | 2 | 60 | 114 | 50 | 112 | 5.0e-12 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95747.t1 | A0A072UIF2 | 97.4 | 114 | 3 | 0 | 1 | 114 | 1 | 114 | 2.1e-56 | 228.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04925 | MS.gene95747 | 0.807037 | 6.15E-50 | -1.69E-46 |
| MS.gene04926 | MS.gene95747 | 0.803161 | 3.98E-49 | -1.69E-46 |
| MS.gene054277 | MS.gene95747 | 0.80881 | 2.58E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95747.t1 | MTR_4g044463 | 95.513 | 156 | 7 | 0 | 1 | 156 | 1 | 156 | 2.16e-104 | 295 |
| MS.gene95747.t1 | MTR_3g053240 | 85.897 | 156 | 17 | 1 | 1 | 156 | 1 | 151 | 8.33e-90 | 258 |
| MS.gene95747.t1 | MTR_2g024010 | 44.048 | 84 | 47 | 0 | 73 | 156 | 52 | 135 | 2.98e-20 | 81.3 |
| MS.gene95747.t1 | MTR_2g024050 | 44.048 | 84 | 47 | 0 | 73 | 156 | 51 | 134 | 1.06e-19 | 80.1 |
| MS.gene95747.t1 | MTR_2g024030 | 31.410 | 156 | 85 | 3 | 1 | 156 | 1 | 134 | 3.20e-19 | 78.6 |
| MS.gene95747.t1 | MTR_2g100600 | 32.484 | 157 | 82 | 5 | 1 | 156 | 1 | 134 | 6.04e-18 | 75.5 |
| MS.gene95747.t1 | MTR_5g093370 | 32.941 | 85 | 54 | 2 | 74 | 155 | 56 | 140 | 8.98e-13 | 62.8 |
| MS.gene95747.t1 | MTR_5g093340 | 32.941 | 85 | 54 | 2 | 74 | 155 | 56 | 140 | 1.07e-12 | 62.4 |
| MS.gene95747.t1 | MTR_2g085360 | 37.383 | 107 | 62 | 2 | 55 | 156 | 37 | 143 | 3.69e-12 | 60.5 |
| MS.gene95747.t1 | MTR_7g025060 | 35.211 | 71 | 44 | 1 | 87 | 155 | 71 | 141 | 8.97e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95747.t1 | AT1G50720 | 42.424 | 165 | 75 | 4 | 1 | 156 | 1 | 154 | 1.10e-41 | 136 |
| MS.gene95747.t1 | AT4G26880 | 56.364 | 110 | 38 | 2 | 57 | 156 | 43 | 152 | 4.59e-37 | 125 |
| MS.gene95747.t1 | AT5G55110 | 43.827 | 162 | 81 | 5 | 3 | 156 | 4 | 163 | 4.28e-35 | 120 |
| MS.gene95747.t1 | AT1G11925 | 33.766 | 154 | 85 | 2 | 3 | 156 | 4 | 140 | 1.49e-25 | 95.1 |
| MS.gene95747.t1 | AT1G50650 | 43.590 | 78 | 42 | 1 | 81 | 156 | 61 | 138 | 6.40e-16 | 71.2 |
| MS.gene95747.t1 | AT1G53130 | 29.341 | 167 | 98 | 6 | 3 | 156 | 9 | 168 | 3.27e-14 | 66.6 |
Find 30 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATATTCGTTGTGAAAT+TGG | 0.186109 | 4.1:+54292048 | MS.gene95747:CDS |
| CAACAACAAGTGCATTGATT+TGG | 0.256529 | 4.1:+54291915 | MS.gene95747:CDS |
| TGACAATGAATAGGTGCATT+AGG | 0.291524 | 4.1:-54291841 | None:intergenic |
| GTTGCAACATGTAGCGTTCT+TGG | 0.292752 | 4.1:-54291896 | None:intergenic |
| GATGAGATTTGCGACTTATA+TGG | 0.298274 | 4.1:+54291871 | MS.gene95747:CDS |
| AACAACAAGTGCATTGATTT+GGG | 0.354325 | 4.1:+54291916 | MS.gene95747:CDS |
| GACAATGAATAGGTGCATTA+GGG | 0.365229 | 4.1:-54291840 | None:intergenic |
| ATTGGTCAGTATTGTGTCTA+TGG | 0.372629 | 4.1:+54292066 | MS.gene95747:CDS |
| GTGATGAACCGATGCCGGTT+TGG | 0.398173 | 4.1:-54291725 | None:intergenic |
| GGATTAGTGGTTGTTGCCCT+TGG | 0.406238 | 4.1:-54291759 | None:intergenic |
| GATTAGTGGTTGTTGCCCTT+GGG | 0.408760 | 4.1:-54291758 | None:intergenic |
| AGGCGACTTACCCTCTTTGA+AGG | 0.442528 | 4.1:-54291805 | None:intergenic |
| TTGAGACAAACTATGATAAG+AGG | 0.460251 | 4.1:+54292013 | MS.gene95747:CDS |
| TCTTGCTTGTGACAATGAAT+AGG | 0.483443 | 4.1:-54291850 | None:intergenic |
| CACACAAAACGAAACCAAAC+CGG | 0.492299 | 4.1:+54291711 | MS.gene95747:CDS |
| GAATCGTGATGAACCGATGC+CGG | 0.512807 | 4.1:-54291730 | None:intergenic |
| AAACGAAACCAAACCGGCAT+CGG | 0.516449 | 4.1:+54291717 | MS.gene95747:CDS |
| CAAGTTTCTCCCTTCAAAGA+GGG | 0.553528 | 4.1:+54291795 | MS.gene95747:CDS |
| GGCGACTTACCCTCTTTGAA+GGG | 0.579648 | 4.1:-54291804 | None:intergenic |
| CATCACGATTCTCTCTCCCA+AGG | 0.581392 | 4.1:+54291742 | MS.gene95747:CDS |
| AACTATGATAAGAGGCATTG+TGG | 0.588533 | 4.1:+54292021 | MS.gene95747:CDS |
| ACAAGTTTCTCCCTTCAAAG+AGG | 0.591246 | 4.1:+54291794 | MS.gene95747:CDS |
| TACACTCAAACATGTTGTAG+AGG | 0.609568 | 4.1:+54291982 | MS.gene95747:CDS |
| ATCACGATTCTCTCTCCCAA+GGG | 0.616942 | 4.1:+54291743 | MS.gene95747:CDS |
| ATGATAAGAGGCATTGTGGA+GGG | 0.620594 | 4.1:+54292025 | MS.gene95747:CDS |
| GGATATGATAAGCATAATTG+TGG | 0.628965 | 4.1:+54291937 | MS.gene95747:CDS |
| GATAGTGAGGGACAAAGCCA+TGG | 0.638314 | 4.1:-54291674 | None:intergenic |
| AATAATCATAGCAATAACCA+TGG | 0.640365 | 4.1:+54291657 | MS.gene95747:CDS |
| TATGATAAGAGGCATTGTGG+AGG | 0.644648 | 4.1:+54292024 | MS.gene95747:CDS |
| TATTGTGTCTATGGAATGTG+CGG | 0.684868 | 4.1:+54292075 | MS.gene95747:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTATGATTATTATTGCTTTT+AGG | - | chr4.1:54291648-54291667 | None:intergenic | 20.0% |
| ! | AATAATCATAGCAATAACCA+TGG | + | chr4.1:54291657-54291676 | MS.gene95747:CDS | 25.0% |
| GGATATGATAAGCATAATTG+TGG | + | chr4.1:54291937-54291956 | MS.gene95747:CDS | 30.0% | |
| TGCAATATTCGTTGTGAAAT+TGG | + | chr4.1:54292048-54292067 | MS.gene95747:CDS | 30.0% | |
| TTGAGACAAACTATGATAAG+AGG | + | chr4.1:54292013-54292032 | MS.gene95747:CDS | 30.0% | |
| !! | AACAACAAGTGCATTGATTT+GGG | + | chr4.1:54291916-54291935 | MS.gene95747:CDS | 30.0% |
| ATTGGTCAGTATTGTGTCTA+TGG | + | chr4.1:54292066-54292085 | MS.gene95747:CDS | 35.0% | |
| GACAATGAATAGGTGCATTA+GGG | - | chr4.1:54291843-54291862 | None:intergenic | 35.0% | |
| GATGAGATTTGCGACTTATA+TGG | + | chr4.1:54291871-54291890 | MS.gene95747:CDS | 35.0% | |
| TACACTCAAACATGTTGTAG+AGG | + | chr4.1:54291982-54292001 | MS.gene95747:CDS | 35.0% | |
| TATTGTGTCTATGGAATGTG+CGG | + | chr4.1:54292075-54292094 | MS.gene95747:CDS | 35.0% | |
| TCTTGCTTGTGACAATGAAT+AGG | - | chr4.1:54291853-54291872 | None:intergenic | 35.0% | |
| TGACAATGAATAGGTGCATT+AGG | - | chr4.1:54291844-54291863 | None:intergenic | 35.0% | |
| ! | TTTTTCTCATGGAGGATTAG+TGG | - | chr4.1:54291775-54291794 | None:intergenic | 35.0% |
| !! | AACTATGATAAGAGGCATTG+TGG | + | chr4.1:54292021-54292040 | MS.gene95747:CDS | 35.0% |
| !! | CAACAACAAGTGCATTGATT+TGG | + | chr4.1:54291915-54291934 | MS.gene95747:CDS | 35.0% |
| !! | GAAACTTGTTTTTCTCATGG+AGG | - | chr4.1:54291783-54291802 | None:intergenic | 35.0% |
| !! | GGAGAAACTTGTTTTTCTCA+TGG | - | chr4.1:54291786-54291805 | None:intergenic | 35.0% |
| !! | GGTTTTCATTGTGATAGTGA+GGG | - | chr4.1:54291689-54291708 | None:intergenic | 35.0% |
| !! | TGGTTTTCATTGTGATAGTG+AGG | - | chr4.1:54291690-54291709 | None:intergenic | 35.0% |
| !! | TTTGGTTTCGTTTTGTGTGA+TGG | - | chr4.1:54291710-54291729 | None:intergenic | 35.0% |
| ACAAGTTTCTCCCTTCAAAG+AGG | + | chr4.1:54291794-54291813 | MS.gene95747:CDS | 40.0% | |
| CAAGTTTCTCCCTTCAAAGA+GGG | + | chr4.1:54291795-54291814 | MS.gene95747:CDS | 40.0% | |
| CACACAAAACGAAACCAAAC+CGG | + | chr4.1:54291711-54291730 | MS.gene95747:CDS | 40.0% | |
| !! | ATGATAAGAGGCATTGTGGA+GGG | + | chr4.1:54292025-54292044 | MS.gene95747:CDS | 40.0% |
| !! | TATGATAAGAGGCATTGTGG+AGG | + | chr4.1:54292024-54292043 | MS.gene95747:CDS | 40.0% |
| AAACGAAACCAAACCGGCAT+CGG | + | chr4.1:54291717-54291736 | MS.gene95747:CDS | 45.0% | |
| ATCACGATTCTCTCTCCCAA+GGG | + | chr4.1:54291743-54291762 | MS.gene95747:CDS | 45.0% | |
| GATTAGTGGTTGTTGCCCTT+GGG | - | chr4.1:54291761-54291780 | None:intergenic | 45.0% | |
| GTTGCAACATGTAGCGTTCT+TGG | - | chr4.1:54291899-54291918 | None:intergenic | 45.0% | |
| !!! | CATTAGGGTTTTGAGCTAGG+AGG | - | chr4.1:54291828-54291847 | None:intergenic | 45.0% |
| !!! | GTGCATTAGGGTTTTGAGCT+AGG | - | chr4.1:54291831-54291850 | None:intergenic | 45.0% |
| AGGCGACTTACCCTCTTTGA+AGG | - | chr4.1:54291808-54291827 | None:intergenic | 50.0% | |
| CATCACGATTCTCTCTCCCA+AGG | + | chr4.1:54291742-54291761 | MS.gene95747:CDS | 50.0% | |
| GAATCGTGATGAACCGATGC+CGG | - | chr4.1:54291733-54291752 | None:intergenic | 50.0% | |
| GATAGTGAGGGACAAAGCCA+TGG | - | chr4.1:54291677-54291696 | None:intergenic | 50.0% | |
| GGCGACTTACCCTCTTTGAA+GGG | - | chr4.1:54291807-54291826 | None:intergenic | 50.0% | |
| ! | GGATTAGTGGTTGTTGCCCT+TGG | - | chr4.1:54291762-54291781 | None:intergenic | 50.0% |
| ! | GTGATGAACCGATGCCGGTT+TGG | - | chr4.1:54291728-54291747 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 54291637 | 54292107 | 54291637 | ID=MS.gene95747 |
| chr4.1 | mRNA | 54291637 | 54292107 | 54291637 | ID=MS.gene95747.t1;Parent=MS.gene95747 |
| chr4.1 | exon | 54291637 | 54292107 | 54291637 | ID=MS.gene95747.t1.exon1;Parent=MS.gene95747.t1 |
| chr4.1 | CDS | 54291637 | 54292107 | 54291637 | ID=cds.MS.gene95747.t1;Parent=MS.gene95747.t1 |
| Gene Sequence |
| Protein sequence |
