AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95866

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95866.t1 MTR_8g106240 92.916 367 9 6 1 363 1 354 0.0 688
MS.gene95866.t1 MTR_8g106240 91.606 274 6 6 1 270 1 261 1.27e-176 492
MS.gene95866.t1 MTR_8g106240 90.456 241 6 6 1 237 1 228 5.22e-148 417
MS.gene95866.t1 MTR_6g082620 55.172 377 119 12 15 362 4 359 1.17e-120 353
MS.gene95866.t1 MTR_1g019200 44.262 122 66 2 235 356 583 702 1.76e-30 123
MS.gene95866.t1 MTR_3g108808 44.186 129 65 3 237 359 405 532 7.77e-29 117
MS.gene95866.t1 MTR_1g066430 45.217 115 58 3 243 356 583 693 1.12e-27 114
MS.gene95866.t1 MTR_2g078450 43.590 117 59 1 252 361 401 517 1.19e-26 111
MS.gene95866.t1 MTR_1g009680 38.514 148 68 3 233 357 323 470 5.81e-26 108
MS.gene95866.t1 MTR_7g100950 43.182 132 69 4 226 356 419 545 8.53e-26 108
MS.gene95866.t1 MTR_7g100950 43.939 132 66 4 226 356 419 543 1.24e-25 108
MS.gene95866.t1 MTR_2g078440 47.664 107 52 3 252 358 194 296 2.42e-24 102
MS.gene95866.t1 MTR_1g066400 45.872 109 54 3 249 356 421 525 6.12e-24 103
MS.gene95866.t1 MTR_4g085630 43.590 117 65 1 245 361 441 556 1.15e-23 102
MS.gene95866.t1 MTR_2g076290 37.931 116 61 3 252 358 214 327 2.30e-19 88.2
MS.gene95866.t1 MTR_3g025940 39.175 97 58 1 262 358 348 443 1.72e-18 86.7
MS.gene95866.t1 MTR_8g037150 34.694 98 63 1 262 359 144 240 1.15e-16 79.0
MS.gene95866.t1 MTR_3g088100 39.000 100 50 3 265 360 111 203 1.37e-15 75.1
MS.gene95866.t1 MTR_4g065670 35.714 112 69 2 252 361 132 242 1.57e-15 75.9
MS.gene95866.t1 MTR_5g016960 31.579 133 87 4 226 357 464 593 2.47e-15 77.8
MS.gene95866.t1 MTR_1g114240 29.375 160 97 5 209 361 178 328 2.54e-15 76.6
MS.gene95866.t1 MTR_4g065670 35.780 109 67 2 255 361 142 249 3.07e-15 75.1
MS.gene95866.t1 MTR_1g114240 35.455 110 66 2 252 361 230 334 3.56e-15 76.3
MS.gene95866.t1 MTR_5g079050 40.278 72 42 1 285 356 284 354 7.67e-15 75.5
MS.gene95866.t1 MTR_5g078900 38.356 73 44 1 285 357 284 355 2.77e-14 73.9
MS.gene95866.t1 MTR_8g036640 32.990 97 63 2 253 349 134 228 3.25e-13 69.7
MS.gene95866.t1 MTR_2g076240 33.566 143 51 6 225 358 71 178 1.17e-12 66.2
MS.gene95866.t1 MTR_5g016920 29.012 162 85 7 200 357 7 142 1.66e-12 65.1
MS.gene95866.t1 MTR_5g016980 37.349 83 48 3 282 362 254 334 5.79e-12 66.6
MS.gene95866.t1 MTR_5g016940 38.356 73 39 1 285 357 275 341 1.80e-11 65.1
MS.gene95866.t1 MTR_3g088080 46.667 45 24 0 313 357 36 80 8.51e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95866.t1 AT1G73760 44.262 366 160 9 13 356 17 360 1.12e-85 264
MS.gene95866.t1 AT1G17970 36.883 385 193 12 8 362 3 367 3.06e-56 188
MS.gene95866.t1 AT1G73760 41.786 280 122 8 13 270 17 277 9.71e-49 166
MS.gene95866.t1 AT5G10650 40.476 126 71 1 235 356 391 516 1.46e-31 125
MS.gene95866.t1 AT5G10650 40.476 126 71 1 235 356 391 516 1.46e-31 125
MS.gene95866.t1 AT4G31450 40.458 131 69 2 234 356 360 489 4.06e-30 120
MS.gene95866.t1 AT4G31450 40.458 131 69 2 234 356 360 489 4.06e-30 120
MS.gene95866.t1 AT4G34040 46.429 112 59 1 245 356 550 660 2.50e-28 116
MS.gene95866.t1 AT5G24870 33.696 184 109 3 186 356 326 509 1.36e-27 114
MS.gene95866.t1 AT5G24870 33.696 184 109 3 186 356 326 509 1.36e-27 114
MS.gene95866.t1 AT5G24870 33.696 184 109 3 186 356 326 509 1.36e-27 114
MS.gene95866.t1 AT2G15530 46.154 117 61 1 245 359 584 700 1.49e-27 114
MS.gene95866.t1 AT2G15530 46.154 117 61 1 245 359 584 700 1.49e-27 114
MS.gene95866.t1 AT2G15530 46.154 117 61 1 245 359 584 700 1.49e-27 114
MS.gene95866.t1 AT2G15530 46.154 117 61 1 245 359 584 700 1.49e-27 114
MS.gene95866.t1 AT2G15530 46.154 117 61 1 245 359 584 700 1.49e-27 114
MS.gene95866.t1 AT2G15530 46.154 117 61 1 245 359 562 678 1.49e-27 114
MS.gene95866.t1 AT1G45180 42.748 131 72 2 226 356 458 585 1.05e-26 111
MS.gene95866.t1 AT5G42940 39.695 131 74 3 228 356 551 678 1.70e-26 111
MS.gene95866.t1 AT5G42940 39.695 131 74 3 228 356 551 678 1.70e-26 111
MS.gene95866.t1 AT1G45180 42.748 131 72 2 226 356 506 633 1.79e-26 111
MS.gene95866.t1 AT1G45180 42.748 131 72 2 226 356 506 633 1.79e-26 111
MS.gene95866.t1 AT1G45180 42.748 131 72 2 226 356 506 633 1.79e-26 111
MS.gene95866.t1 AT1G53190 38.406 138 75 4 230 359 351 486 3.39e-26 109
MS.gene95866.t1 AT1G53190 38.406 138 75 4 230 359 351 486 3.39e-26 109
MS.gene95866.t1 AT2G15530 45.614 114 60 1 245 356 584 697 2.33e-25 108
MS.gene95866.t1 AT3G15070 36.937 111 67 1 252 359 362 472 2.03e-22 98.6
MS.gene95866.t1 AT3G15070 36.937 111 67 1 252 359 362 472 2.03e-22 98.6
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 429 534 2.53e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 445 550 2.71e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 445 550 2.71e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 445 550 2.71e-20 92.8
MS.gene95866.t1 AT2G37150 45.872 109 55 3 249 356 482 587 3.44e-20 92.4
MS.gene95866.t1 AT3G63530 35.714 98 62 1 262 359 145 241 2.50e-16 78.2
MS.gene95866.t1 AT3G63530 35.714 98 62 1 262 359 145 241 2.50e-16 78.2
MS.gene95866.t1 AT1G36950 37.143 105 58 2 255 357 24 122 3.80e-16 77.0
MS.gene95866.t1 AT4G00070 38.144 97 52 2 255 349 88 178 8.01e-16 75.1
MS.gene95866.t1 AT3G47180 40.217 92 51 3 265 355 115 203 5.14e-15 73.6
MS.gene95866.t1 AT3G19910 33.000 100 64 1 261 360 237 333 1.80e-14 73.9
MS.gene95866.t1 AT5G52140 37.500 104 58 2 263 359 175 278 3.36e-14 72.4
MS.gene95866.t1 AT5G67120 36.082 97 56 2 265 360 181 272 3.92e-14 72.4
MS.gene95866.t1 AT5G10650 35.955 89 53 1 235 319 391 479 2.91e-13 71.2

Find 58 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTTGTTGATTTGGTTTC+TGG 0.069335 4.3:+924312 None:intergenic
TCCGGAAAAGAGAAGGTTTC+TGG 0.229241 4.3:+923499 None:intergenic
TTCTGTTTCTGTTTCCGAAA+TGG 0.286799 4.3:+924279 None:intergenic
CACGTGATCTTTACAGAGAT+TGG 0.310639 4.3:-922707 MS.gene95866:CDS
GCAGCTACCTTCAGAGGTTT+AGG 0.311213 4.3:-924124 MS.gene95866:CDS
TGAGGTTGTTGTTGTTTCAT+TGG 0.320662 4.3:+924186 None:intergenic
TCTGTTTCTGTTTCCGAAAT+GGG 0.323731 4.3:+924280 None:intergenic
CACTGCTGGTGCTTCTCAAC+AGG 0.353298 4.3:-924098 MS.gene95866:CDS
CAGAGATGAAATTGTGGACT+TGG 0.368102 4.3:+924216 None:intergenic
GTTTGGTGTGGTCCTGGAAT+TGG 0.370161 4.3:-923938 MS.gene95866:CDS
AGCTTAGTCTTCCTTATGTA+AGG 0.382634 4.3:+922451 None:intergenic
CTTGAGCTCGGTGAGAGAAT+TGG 0.385669 4.3:-922511 MS.gene95866:CDS
CAGAGCAACAACATATGAAA+TGG 0.397557 4.3:-924345 MS.gene95866:CDS
CAAGATGTTTGGTGTGGTCC+TGG 0.397639 4.3:-923944 MS.gene95866:CDS
CTGTTTCCGAAATGGGTTTG+TGG 0.416794 4.3:+924287 None:intergenic
CTTTCAATGTATACAACAAT+GGG 0.432717 4.3:-921783 MS.gene95866:CDS
GGTAGACAGCAGTGCGCCTT+CGG 0.433538 4.3:+923521 None:intergenic
AGAATTGGTTATGTGAATAC+TGG 0.435476 4.3:-922496 MS.gene95866:CDS
TCTGCTGAAAATCCAATTCC+AGG 0.438438 4.3:+923926 None:intergenic
GCTTCAAGGAAGAATACTAA+TGG 0.441507 4.3:-922745 MS.gene95866:CDS
TTCTCAACAGGTGTCGGTGC+CGG 0.464786 4.3:-924086 MS.gene95866:CDS
CCGGAAAAGAGAAGGTTTCT+GGG 0.472828 4.3:+923500 None:intergenic
CTGCCCTGTGTGTAAGCAGC+AGG 0.483447 4.3:-921744 MS.gene95866:CDS
TGATTCGTGCTTCAGCAGAT+TGG 0.488872 4.3:-924060 MS.gene95866:CDS
AGCCTTCATCTTCCTTCCGA+AGG 0.489286 4.3:-923537 MS.gene95866:CDS
AGAGGTTTAGGATGCACTGC+TGG 0.490523 4.3:-924112 MS.gene95866:CDS
TTGAAGATAATGGCGCTTCA+AGG 0.491059 4.3:-922759 MS.gene95866:intron
TGGACTTGGAGCGTGTGGAT+TGG 0.504134 4.3:+924230 None:intergenic
AAAATGTTGGGATCGGTGTC+CGG 0.509723 4.3:+923481 None:intergenic
TTGTTGATTTGGTTTCTGGA+TGG 0.520614 4.3:+924316 None:intergenic
CTTCAAGGAAGAATACTAAT+GGG 0.521708 4.3:-922744 MS.gene95866:CDS
ATGAGATGGAACCTTACATA+AGG 0.523941 4.3:-922462 MS.gene95866:CDS
AAGCAGATGATGAGCTTGCA+AGG 0.525631 4.3:-921829 MS.gene95866:CDS
ACTTTCAATGTATACAACAA+TGG 0.529407 4.3:-921784 MS.gene95866:CDS
GAGCATGCTGTGAAAATGTT+GGG 0.537021 4.3:+923469 None:intergenic
TCTGCAGCAGCTACCTTCAG+AGG 0.546370 4.3:-924130 MS.gene95866:CDS
GACAGCAGTGCGCCTTCGGA+AGG 0.548094 4.3:+923525 None:intergenic
TGGACTTAAAGAAGATGAGA+TGG 0.549079 4.3:-922476 MS.gene95866:CDS
TGGTGCTTCTCAACAGGTGT+CGG 0.554363 4.3:-924092 MS.gene95866:CDS
TCAGCAGATTGGCCTCATCA+AGG 0.557093 4.3:-924049 MS.gene95866:CDS
AGAGCATGCTGTGAAAATGT+TGG 0.565371 4.3:+923468 None:intergenic
CAGCAGATTGGCCTCATCAA+GGG 0.570341 4.3:-924048 MS.gene95866:CDS
GCAACCTGCTGCTTACACAC+AGG 0.572960 4.3:+921740 None:intergenic
CGCCTTCGGAAGGAAGATGA+AGG 0.573947 4.3:+923535 None:intergenic
CAACCTGCTGCTTACACACA+GGG 0.578724 4.3:+921741 None:intergenic
ATCGGTGTCCGGAAAAGAGA+AGG 0.579710 4.3:+923492 None:intergenic
TGACAGCAATTGCTTGAGCT+CGG 0.580670 4.3:-922523 MS.gene95866:intron
TTTGTTAATGGGTTGTTGTG+AGG 0.581700 4.3:+924168 None:intergenic
AGTGCATCCTAAACCTCTGA+AGG 0.584439 4.3:+924117 None:intergenic
GAGAAGCAGAGATGAAATTG+TGG 0.590057 4.3:+924210 None:intergenic
TGTTGATAACATGTCATATG+AGG 0.603689 4.3:-922676 MS.gene95866:intron
TGCTGTGAAAATGTTGGGAT+CGG 0.608642 4.3:+923474 None:intergenic
AATTGTGGACTTGGAGCGTG+TGG 0.637667 4.3:+924225 None:intergenic
GCTGAAGCACGAATCACTGC+CGG 0.639164 4.3:+924067 None:intergenic
CAAGGAAGAATACTAATGGG+AGG 0.689238 4.3:-922741 MS.gene95866:CDS
GAAATATCTCTGATATGACG+AGG 0.695301 4.3:+923427 None:intergenic
AAATATCTCTGATATGACGA+GGG 0.711372 4.3:+923428 None:intergenic
CGACAAGATAACTCACAGAG+AGG 0.725536 4.3:-923834 MS.gene95866:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAGAAATGATTAT+TGG + chr4.3:923462-923481 None:intergenic 10.0%
!! ATGTATATCATAAATTTAAT+TGG + chr4.3:924216-924235 None:intergenic 10.0%
!! AAAAAATAACCTAAATACAT+AGG - chr4.3:924087-924106 MS.gene95866:CDS 15.0%
!! CAAAATCATGAATTTAAAAA+TGG - chr4.3:924149-924168 MS.gene95866:CDS 15.0%
!!! CATTTTTAAATTCATGATTT+TGG + chr4.3:924151-924170 None:intergenic 15.0%
!!! TAATTTTAATTTATGAGTAC+AGG - chr4.3:924239-924258 MS.gene95866:CDS 15.0%
!! AAAAAGAAAGTAAATTACCA+TGG + chr4.3:922819-922838 None:intergenic 20.0%
!! AAACTTGATCATAACTATAT+TGG - chr4.3:922539-922558 MS.gene95866:intron 20.0%
!! AATCATGAATTTAAAAATGG+AGG - chr4.3:924152-924171 MS.gene95866:CDS 20.0%
!! ATCATGAATTTAAAAATGGA+GGG - chr4.3:924153-924172 MS.gene95866:CDS 20.0%
!!! AAAGTTGATCTTTTTGTTAA+TGG + chr4.3:921947-921966 None:intergenic 20.0%
!!! AAGTTGATCTTTTTGTTAAT+GGG + chr4.3:921946-921965 None:intergenic 20.0%
!!! ACTAAATTCTAAAATTGGAT+TGG - chr4.3:923000-923019 MS.gene95866:intron 20.0%
!!! ATAAAAATGATCAAAGTACT+TGG + chr4.3:922891-922910 None:intergenic 20.0%
!!! TCATCATATAATTTTGTCAA+TGG - chr4.3:924063-924082 MS.gene95866:CDS 20.0%
! AAAATCGTAGATTGAGAAAT+AGG + chr4.3:923804-923823 None:intergenic 25.0%
! AAATAACCTAAATACATAGG+TGG - chr4.3:924090-924109 MS.gene95866:CDS 25.0%
! AAATCGTAGATTGAGAAATA+GGG + chr4.3:923803-923822 None:intergenic 25.0%
! AATTAATGCCTACTTAAAGT+AGG - chr4.3:923092-923111 MS.gene95866:intron 25.0%
! ACTTTCAATGTATACAACAA+TGG - chr4.3:924316-924335 MS.gene95866:CDS 25.0%
! ATGTTGTAAATTTCTTTGCA+TGG - chr4.3:923211-923230 MS.gene95866:intron 25.0%
! CATAAAAACTAAGCAAAATC+TGG - chr4.3:922778-922797 MS.gene95866:intron 25.0%
! CTTTCAATGTATACAACAAT+GGG - chr4.3:924317-924336 MS.gene95866:CDS 25.0%
! TCTTGTGTATTCTTTCATTA+AGG - chr4.3:922727-922746 MS.gene95866:CDS 25.0%
! TGTATAGCATTTGAATGTAT+TGG - chr4.3:922975-922994 MS.gene95866:intron 25.0%
! TTCTTCTATGTACATTAGTT+AGG - chr4.3:922348-922367 MS.gene95866:intron 25.0%
! TTGCATATTCTCATTATTGT+TGG - chr4.3:923314-923333 MS.gene95866:intron 25.0%
!! GAACATCACAATTTTCAAAA+TGG + chr4.3:922331-922350 None:intergenic 25.0%
!! GATTTGGTATATTCTATTCA+AGG + chr4.3:923030-923049 None:intergenic 25.0%
!! TTGATTGCGATTTTTATGTT+AGG - chr4.3:923157-923176 MS.gene95866:intron 25.0%
!!! ACATGATTTTTTCAAGAAGT+AGG - chr4.3:923182-923201 MS.gene95866:intron 25.0%
!!! ATCAGACATTTTGAAAATTG+TGG - chr4.3:924123-924142 MS.gene95866:CDS 25.0%
!!! TCAGACATTTTGAAAATTGT+GGG - chr4.3:924124-924143 MS.gene95866:CDS 25.0%
!!! TGGCTACTAAATTCTAAAAT+TGG - chr4.3:922995-923014 MS.gene95866:intron 25.0%
!!! TGTTGGAATTTTGAAGATAA+TGG - chr4.3:923331-923350 MS.gene95866:intron 25.0%
!!! TTTTAATTTATGAGTACAGG+AGG - chr4.3:924242-924261 MS.gene95866:CDS 25.0%
AAATATCTCTGATATGACGA+GGG + chr4.3:922675-922694 None:intergenic 30.0%
AATCGTAGATTGAGAAATAG+GGG + chr4.3:923802-923821 None:intergenic 30.0%
ACTGTTATCAGAAACCAAAA+TGG + chr4.3:922491-922510 None:intergenic 30.0%
AGAATTGGTTATGTGAATAC+TGG - chr4.3:923604-923623 MS.gene95866:intron 30.0%
CTTCAAGGAAGAATACTAAT+GGG - chr4.3:923356-923375 MS.gene95866:intron 30.0%
GCGATGTTAAAAACATGATT+TGG + chr4.3:923046-923065 None:intergenic 30.0%
TAACTTAGCAAAGACTTGAT+TGG + chr4.3:923273-923292 None:intergenic 30.0%
TGTTGAGTAAGTCAATATGA+TGG - chr4.3:923241-923260 MS.gene95866:intron 30.0%
! ACAGACATCTATGATTAGTT+TGG - chr4.3:922420-922439 MS.gene95866:CDS 30.0%
! AGTAACTTGCATGATTAGTA+AGG + chr4.3:922947-922966 None:intergenic 30.0%
! AGTTTTACAAACTAACTACC+TGG + chr4.3:923729-923748 None:intergenic 30.0%
! GTCGGTCAATTTTAAATTTG+AGG + chr4.3:923831-923850 None:intergenic 30.0%
! GTTAAACTTCATCACCATTT+TGG - chr4.3:922474-922493 MS.gene95866:CDS 30.0%
! TGGTGATGAAGTTTAACATA+AGG + chr4.3:922471-922490 None:intergenic 30.0%
!! TGTTGATAACATGTCATATG+AGG - chr4.3:923424-923443 MS.gene95866:CDS 30.0%
!! TTTGTTGTTGATTTGGTTTC+TGG + chr4.3:921791-921810 None:intergenic 30.0%
!!! ATTCTTGTGCTTCTTTTTTC+TGG + chr4.3:922085-922104 None:intergenic 30.0%
!!! GATTGGATTTGTTTTGTGTT+AGG + chr4.3:921856-921875 None:intergenic 30.0%
!!! GTGTTGATTTTCAAGATGTT+TGG - chr4.3:922145-922164 MS.gene95866:intron 30.0%
!!! TGTGGTTTTTGTTGTTGATT+TGG + chr4.3:921798-921817 None:intergenic 30.0%
AACACCTTCCTACTTTAAGT+AGG + chr4.3:923103-923122 None:intergenic 35.0%
AATGCATAGGTAGAAGGAAA+AGG + chr4.3:923853-923872 None:intergenic 35.0%
AATGCCTACTTAAAGTAGGA+AGG - chr4.3:923096-923115 MS.gene95866:intron 35.0%
ATCGTAGATTGAGAAATAGG+GGG + chr4.3:923801-923820 None:intergenic 35.0%
ATGAGATGGAACCTTACATA+AGG - chr4.3:923638-923657 MS.gene95866:intron 35.0%
CAAAAACCACAAACCCATTT+CGG - chr4.3:921807-921826 MS.gene95866:CDS 35.0%
CAGAGCAACAACATATGAAA+TGG - chr4.3:921755-921774 MS.gene95866:CDS 35.0%
CTTAGTTGGTTATATGACAC+AGG - chr4.3:923910-923929 MS.gene95866:CDS 35.0%
GAAATATCTCTGATATGACG+AGG + chr4.3:922676-922695 None:intergenic 35.0%
GCTTCAAGGAAGAATACTAA+TGG - chr4.3:923355-923374 MS.gene95866:intron 35.0%
TGGACTTAAAGAAGATGAGA+TGG - chr4.3:923624-923643 MS.gene95866:intron 35.0%
TGTTGAAGATGTTGTAAGTG+AGG - chr4.3:923884-923903 MS.gene95866:CDS 35.0%
! AGCTTAGTCTTCCTTATGTA+AGG + chr4.3:923652-923671 None:intergenic 35.0%
! ATTTTCACAGCATGCTCTTT+TGG - chr4.3:922637-922656 MS.gene95866:intron 35.0%
! TTCGTCTGATGATTTTTCTG+AGG - chr4.3:922693-922712 MS.gene95866:CDS 35.0%
!! CTTAAAGTAGGAAGGTGTTA+TGG - chr4.3:923104-923123 MS.gene95866:intron 35.0%
!! GATTTTCAAGATGTTTGGTG+TGG - chr4.3:922150-922169 MS.gene95866:intron 35.0%
!! TCTGTTTCTGTTTCCGAAAT+GGG + chr4.3:921823-921842 None:intergenic 35.0%
!! TGAGGTTGTTGTTGTTTCAT+TGG + chr4.3:921917-921936 None:intergenic 35.0%
!! TTCTGTTTCTGTTTCCGAAA+TGG + chr4.3:921824-921843 None:intergenic 35.0%
!! TTGTTGATTTGGTTTCTGGA+TGG + chr4.3:921787-921806 None:intergenic 35.0%
!! TTTGTTAATGGGTTGTTGTG+AGG + chr4.3:921935-921954 None:intergenic 35.0%
!!! TGGATTTGTTTTGTGTTAGG+AGG + chr4.3:921853-921872 None:intergenic 35.0%
ACAAAGATGCATGCAAGTAC+TGG + chr4.3:923526-923545 None:intergenic 40.0%
AGAGCATGCTGTGAAAATGT+TGG + chr4.3:922635-922654 None:intergenic 40.0%
CAAGGAAGAATACTAATGGG+AGG - chr4.3:923359-923378 MS.gene95866:intron 40.0%
CACGTGATCTTTACAGAGAT+TGG - chr4.3:923393-923412 MS.gene95866:intron 40.0%
CAGAGATGAAATTGTGGACT+TGG + chr4.3:921887-921906 None:intergenic 40.0%
CATAGGTAGAAGGAAAAGGT+CGG + chr4.3:923849-923868 None:intergenic 40.0%
CATTCAGCCTCAAAATGCAT+AGG + chr4.3:923866-923885 None:intergenic 40.0%
CCTCAAAATGCATAGGTAGA+AGG + chr4.3:923859-923878 None:intergenic 40.0%
CTTCTGCCACCTATGTATTT+AGG + chr4.3:924099-924118 None:intergenic 40.0%
GACAACATATCAACAGCTTG+AGG + chr4.3:923504-923523 None:intergenic 40.0%
GAGAAGCAGAGATGAAATTG+TGG + chr4.3:921893-921912 None:intergenic 40.0%
GAGCATGCTGTGAAAATGTT+GGG + chr4.3:922634-922653 None:intergenic 40.0%
GGTCTGTCTATATCAAACCA+TGG - chr4.3:922799-922818 MS.gene95866:intron 40.0%
TCTGCTGAAAATCCAATTCC+AGG + chr4.3:922177-922196 None:intergenic 40.0%
TGCTGTGAAAATGTTGGGAT+CGG + chr4.3:922629-922648 None:intergenic 40.0%
TGTAAGTGAGGCAACTTAGT+TGG - chr4.3:923896-923915 MS.gene95866:CDS 40.0%
! CCTTCTACCTATGCATTTTG+AGG - chr4.3:923856-923875 MS.gene95866:CDS 40.0%
! TTGAAGATAATGGCGCTTCA+AGG - chr4.3:923341-923360 MS.gene95866:intron 40.0%
AAGCAGATGATGAGCTTGCA+AGG - chr4.3:924271-924290 MS.gene95866:CDS 45.0%
AGTGCATCCTAAACCTCTGA+AGG + chr4.3:921986-922005 None:intergenic 45.0%
CCGGAAAAGAGAAGGTTTCT+GGG + chr4.3:922603-922622 None:intergenic 45.0%
CGACAAGATAACTCACAGAG+AGG - chr4.3:922266-922285 MS.gene95866:intron 45.0%
TCCGGAAAAGAGAAGGTTTC+TGG + chr4.3:922604-922623 None:intergenic 45.0%
TGACAGCAATTGCTTGAGCT+CGG - chr4.3:923577-923596 MS.gene95866:intron 45.0%
TGATTCGTGCTTCAGCAGAT+TGG - chr4.3:922040-922059 MS.gene95866:intron 45.0%
! AAAATGTTGGGATCGGTGTC+CGG + chr4.3:922622-922641 None:intergenic 45.0%
! CAAAAAGTGCAGCATTTGCC+AGG - chr4.3:923708-923727 MS.gene95866:intron 45.0%
! CCCAGAAACCTTCTCTTTTC+CGG - chr4.3:922600-922619 MS.gene95866:intron 45.0%
! CTGTTTCCGAAATGGGTTTG+TGG + chr4.3:921816-921835 None:intergenic 45.0%
!! CTGGTTTTCTTCCCTTGATG+AGG + chr4.3:922066-922085 None:intergenic 45.0%
AATTGTGGACTTGGAGCGTG+TGG + chr4.3:921878-921897 None:intergenic 50.0%
AGCCTTCATCTTCCTTCCGA+AGG - chr4.3:922563-922582 MS.gene95866:intron 50.0%
GCAGCTACCTTCAGAGGTTT+AGG - chr4.3:921976-921995 MS.gene95866:intron 50.0%
TGGTGCTTCTCAACAGGTGT+CGG - chr4.3:922008-922027 MS.gene95866:intron 50.0%
! AGAGGTTTAGGATGCACTGC+TGG - chr4.3:921988-922007 MS.gene95866:intron 50.0%
! ATCGGTGTCCGGAAAAGAGA+AGG + chr4.3:922611-922630 None:intergenic 50.0%
! CAACCTGCTGCTTACACACA+GGG + chr4.3:924362-924381 None:intergenic 50.0%
! CTTGAGCTCGGTGAGAGAAT+TGG - chr4.3:923589-923608 MS.gene95866:intron 50.0%
!! CAAGATGTTTGGTGTGGTCC+TGG - chr4.3:922156-922175 MS.gene95866:intron 50.0%
!! CAGCAGATTGGCCTCATCAA+GGG - chr4.3:922052-922071 MS.gene95866:intron 50.0%
!! GTTTGGTGTGGTCCTGGAAT+TGG - chr4.3:922162-922181 MS.gene95866:intron 50.0%
!! TCAGCAGATTGGCCTCATCA+AGG - chr4.3:922051-922070 MS.gene95866:intron 50.0%
CACTGCTGGTGCTTCTCAAC+AGG - chr4.3:922002-922021 MS.gene95866:intron 55.0%
CGCCTTCGGAAGGAAGATGA+AGG + chr4.3:922568-922587 None:intergenic 55.0%
GCTGAAGCACGAATCACTGC+CGG + chr4.3:922036-922055 None:intergenic 55.0%
TCTGCAGCAGCTACCTTCAG+AGG - chr4.3:921970-921989 MS.gene95866:intron 55.0%
TGGACTTGGAGCGTGTGGAT+TGG + chr4.3:921873-921892 None:intergenic 55.0%
! GCAACCTGCTGCTTACACAC+AGG + chr4.3:924363-924382 None:intergenic 55.0%
!! TTCTCAACAGGTGTCGGTGC+CGG - chr4.3:922014-922033 MS.gene95866:intron 55.0%
CTGCCCTGTGTGTAAGCAGC+AGG - chr4.3:924356-924375 MS.gene95866:CDS 60.0%
GGTAGACAGCAGTGCGCCTT+CGG + chr4.3:922582-922601 None:intergenic 60.0%
GACAGCAGTGCGCCTTCGGA+AGG + chr4.3:922578-922597 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 921727 924395 921727 ID=MS.gene95866
chr4.3 mRNA 921727 924395 921727 ID=MS.gene95866.t1;Parent=MS.gene95866
chr4.3 exon 923835 924395 923835 ID=MS.gene95866.t1.exon1;Parent=MS.gene95866.t1
chr4.3 CDS 923835 924395 923835 ID=cds.MS.gene95866.t1;Parent=MS.gene95866.t1
chr4.3 exon 923408 923557 923408 ID=MS.gene95866.t1.exon2;Parent=MS.gene95866.t1
chr4.3 CDS 923408 923557 923408 ID=cds.MS.gene95866.t1;Parent=MS.gene95866.t1
chr4.3 exon 922677 922775 922677 ID=MS.gene95866.t1.exon3;Parent=MS.gene95866.t1
chr4.3 CDS 922677 922775 922677 ID=cds.MS.gene95866.t1;Parent=MS.gene95866.t1
chr4.3 exon 922393 922539 922393 ID=MS.gene95866.t1.exon4;Parent=MS.gene95866.t1
chr4.3 CDS 922393 922539 922393 ID=cds.MS.gene95866.t1;Parent=MS.gene95866.t1
chr4.3 exon 921727 921861 921727 ID=MS.gene95866.t1.exon5;Parent=MS.gene95866.t1
chr4.3 CDS 921727 921861 921727 ID=cds.MS.gene95866.t1;Parent=MS.gene95866.t1
Gene Sequence

>MS.gene95866

ATGTCTTCTGTGACACAAGATACAAAAACAGAGCAACAACATATGAAATGGAGAAAACCATCCAGAAACCAAATCAACAACAAAAACCACAAACCCATTTCGGAAACAGAAACAGAAACAGATCCTCCTAACACAAAACAAATCCAATCCACACGCTCCAAGTCCACAATTTCATCTCTGCTTCTCTCCAATGAAACAACAACAACCTCACAACAACCCATTAACAAAAAGATCAACTTTTCATCTGCAGCAGCTACCTTCAGAGGTTTAGGATGCACTGCTGGTGCTTCTCAACAGGTGTCGGTGCCGGCAGTGATTCGTGCTTCAGCAGATTGGCCTCATCAAGGGAAGAAAACCAGAAAAAAGAAGCACAAGAATAGTAATGATGCTTCTTCTTCTTCATCTTCAGCAGCTACTTGTGTTGATTTTCAAGATGTTTGGTGTGGTCCTGGAATTGGATTTTCAGCAGATGCTGCTGCTGCATCTGTTGATTGTGTTGTGTCTAAAAAGAATGTGTCTTCAAGAGCTAAAATTGATGTCGACAAGATAACTCACAGAGAGGTAGTAGTATTGCTATTTTTTTTTTATTTTTTTTTTTTTACCATTTTGAAAATTGTGATGTTCTTCTATGTACATTAGTTAGGCTGCATTAATAATGTGTCACTATATATAAACTTGTTTGTTGTGATTTAGACAGACATCTATGATTAGTTTGGATTTTTGTTTGATTTGTGTTTGCTTCCTTATGTTAAACTTCATCACCATTTTGGTTTCTGATAACAGTTTCAAATTTCATAACATGATTATGTACTAAACTTGATCATAACTATATTGGCAGCCTTCATCTTCCTTCCGAAGGCGCACTGCTGTCTACCCAGAAACCTTCTCTTTTCCGGACACCGATCCCAACATTTTCACAGCATGCTCTTTTGGAACTGCGACATACCCTCGTCATATCAGAGATATTTCGTCTGATGATTTTTCTGAGGTTTGCTCATGCTCTTGTGTATTCTTTCATTAAGGTATTCTGATCATTTTTATGATTCTGATTCATAAAAACTAAGCAAAATCTGGTCTGTCTATATCAAACCATGGTAATTTACTTTCTTTTTTTTTTTTTTTTTTTTAAAATCTATAACTTGTTAAATACTGCAAGTTAATCCAAGTACTTTGATCATTTTTATGAATCTGATTCATAAAAACTAAGCAAAACGATACCTTACTAATCATGCAAGTTACTCTATGTTCTGTATAGCATTTGAATGTATTGGCTACTAAATTCTAAAATTGGATTGGCATACCTTGAATAGAATATACCAAATCATGTTTTTAACATCGCTCTTGTTCAATGACAAAATTTGATAAAATTAATGCCTACTTAAAGTAGGAAGGTGTTATGGTTTTGATATATAATTTAGAGAGTTATAATCTTGATTGCGATTTTTATGTTAGGCTACATGATTTTTTCAAGAAGTAGGAAAAAAATGTTGTAAATTTCTTTGCATGGTAGATGATGTTGAGTAAGTCAATATGATGGTTATAACCAATCAAGTCTTTGCTAAGTTATAATTGTAGTTGAAATCTAATTTGCATATTCTCATTATTGTTGGAATTTTGAAGATAATGGCGCTTCAAGGAAGAATACTAATGGGAGGACGATTCAATTCACGTGATCTTTACAGAGATTGGAGACTAGATGTTGATAACATGTCATATGAGGTGACTTCTTTCTCCAATAATCATTTCTATTATTTTTCTATTTTAATAGTTACATCCTCAAGCTGTTGATATGTTGTCCAGTACTTGCATGCATCTTTGTTTTATGTTTGTGTAATTTTCTATTTTGTGTTTGACAGCAATTGCTTGAGCTCGGTGAGAGAATTGGTTATGTGAATACTGGACTTAAAGAAGATGAGATGGAACCTTACATAAGGAAGACTAAGCTTCAGTTTTCAGATGATGCATCAAAGCATCAAGTAGACAAAAAGTGCAGCATTTGCCAGGTAGTTAGTTTGTAAAACTTAATTAGCATAAGTACAATCACTCTGTTTTGCTTTATTCGCGATTTTGTCCCCCTATTTCTCAATCTACGATTTTGATTCCTCAAATTTAAAATTGACCGACCTTTTCCTTCTACCTATGCATTTTGAGGCTGAATGTTGAAGATGTTGTAAGTGAGGCAACTTAGTTGGTTATATGACACAGGCTACGTAGATTCTCACTTAACATTTAATCTTTTTTAATCAAAATATACACTATTGATCAATATCACAAAAACAACTAAATTTAATACAAACAATTCTAAGTGAATTTTCACTTAATAACTCATTTTCATGTCATCATATAATTTTGTCAATGGAAAAAAATAACCTAAATACATAGGTGGCAGAAGAAATATCAGACATTTTGAAAATTGTGGGACCAAAATCATGAATTTAAAAATGGAGGGATCAAAATCAAAAATAAAGCATAAAATTGCACTGTAGCCAATTAAATTTATGATATACATGTTTAATTTTAATTTATGAGTACAGGAGGAGTTTGAAGCAGATGATGAGCTTGCAAGGCTTAACTGTGATCACTTGTATCACTTTCAATGTATACAACAATGGGTTGCACACAAAAATTTCTGCCCTGTGTGTAAGCAGCAGGTTGCAGCTCGACACTGA
Protein sequence

>MS.gene95866.t1

MSSVTQDTKTEQQHMKWRKPSRNQINNKNHKPISETETETDPPNTKQIQSTRSKSTISSLLLSNETTTTSQQPINKKINFSSAAATFRGLGCTAGASQQVSVPAVIRASADWPHQGKKTRKKKHKNSNDASSSSSSAATCVDFQDVWCGPGIGFSADAAAASVDCVVSKKNVSSRAKIDVDKITHREPSSSFRRRTAVYPETFSFPDTDPNIFTACSFGTATYPRHIRDISSDDFSEIMALQGRILMGGRFNSRDLYRDWRLDVDNMSYEQLLELGERIGYVNTGLKEDEMEPYIRKTKLQFSDDASKHQVDKKCSICQEEFEADDELARLNCDHLYHFQCIQQWVAHKNFCPVCKQQVAARH