Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95982.t1 | RHN42281.1 | 82.9 | 111 | 12 | 1 | 1 | 104 | 2745 | 2855 | 2.70E-36 | 161.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95982.t1 | A0A396GQ35 | 82.9 | 111 | 12 | 1 | 1 | 104 | 2745 | 2855 | 2.0e-36 | 161.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049873 | MS.gene95982 | 0.802491 | 5.47E-49 | -1.69E-46 |
| MS.gene050799 | MS.gene95982 | 0.807546 | 4.79E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene95982 | 0.834284 | 3.28E-56 | -1.69E-46 |
| MS.gene053226 | MS.gene95982 | 0.805723 | 1.16E-49 | -1.69E-46 |
| MS.gene053900 | MS.gene95982 | 0.802211 | 6.25E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene95982 | 0.801147 | 1.03E-48 | -1.69E-46 |
| MS.gene056595 | MS.gene95982 | 0.825859 | 3.73E-54 | -1.69E-46 |
| MS.gene058625 | MS.gene95982 | 0.812616 | 3.88E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95982.t1 | MTR_0189s0040 | 61.667 | 120 | 36 | 3 | 1 | 110 | 137 | 256 | 9.43e-31 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTTGTATCAGCTGAATT+TGG | 0.212566 | 2.4:-23852053 | MS.gene95982:CDS |
| ATCAGCTGAATTTGGCTAAA+AGG | 0.217670 | 2.4:-23852045 | MS.gene95982:CDS |
| CTTTCAAAGAAAAGTCTTTC+GGG | 0.265010 | 2.4:-23852094 | MS.gene95982:CDS |
| TCTCAAGCTCCGGTTAATTC+AGG | 0.295800 | 2.4:-23851916 | MS.gene95982:CDS |
| ATTGTTGGACACGTTACCTT+TGG | 0.377222 | 2.4:+23851939 | None:intergenic |
| ACTTTCAAAGAAAAGTCTTT+CGG | 0.378101 | 2.4:-23852095 | MS.gene95982:CDS |
| ATCATTGCCTACTGCAGTGC+TGG | 0.390891 | 2.4:+23851990 | None:intergenic |
| AACCGGAGCTTGAGAATTGT+TGG | 0.439198 | 2.4:+23851924 | None:intergenic |
| TTGTCTATTATCCAGTGATT+CGG | 0.452965 | 2.4:-23852143 | MS.gene95982:intron |
| GTCCAACAATTCTCAAGCTC+CGG | 0.498892 | 2.4:-23851926 | MS.gene95982:CDS |
| GGTTAATTCAGGACACGAAA+AGG | 0.514764 | 2.4:-23851905 | MS.gene95982:CDS |
| CAGTGATTCGGTGATTGAGA+AGG | 0.591135 | 2.4:-23852131 | MS.gene95982:CDS |
| TTCAGGACACGAAAAGGCCA+AGG | 0.604606 | 2.4:-23851899 | MS.gene95982:CDS |
| TCACAAGCCAGCACTGCAGT+AGG | 0.606755 | 2.4:-23851997 | MS.gene95982:intron |
| AACTTACCTCATGTTCATAG+AGG | 0.624152 | 2.4:+23852287 | None:intergenic |
| TTCGAGCCTCTATGAACATG+AGG | 0.624705 | 2.4:-23852293 | MS.gene95982:intron |
| AGGCCAAGGAAAATATCAAG+CGG | 0.686950 | 2.4:-23851885 | MS.gene95982:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACAAATCCAAGAAAAACA+AGG | - | chr2.4:23852328-23852347 | MS.gene95982:CDS | 25.0% |
| ! | AACAAATCCAAGAAAAACAA+GGG | - | chr2.4:23852329-23852348 | MS.gene95982:CDS | 25.0% |
| ! | ACTTTCAAAGAAAAGTCTTT+CGG | - | chr2.4:23852090-23852109 | MS.gene95982:CDS | 25.0% |
| ! | ATCATAAGTAATTGTAGACA+AGG | - | chr2.4:23851934-23851953 | MS.gene95982:CDS | 25.0% |
| ! | TAAAAGGAATAACCAATGTT+GGG | - | chr2.4:23852156-23852175 | MS.gene95982:intron | 25.0% |
| AAAAACAAGGGAGGAAAAAA+GGG | - | chr2.4:23852341-23852360 | MS.gene95982:CDS | 30.0% | |
| CTAAAAGGAATAACCAATGT+TGG | - | chr2.4:23852155-23852174 | MS.gene95982:intron | 30.0% | |
| CTTTCAAAGAAAAGTCTTTC+GGG | - | chr2.4:23852091-23852110 | MS.gene95982:CDS | 30.0% | |
| GGAAAACAAAAATGAAATGG+AGG | - | chr2.4:23851836-23851855 | MS.gene95982:CDS | 30.0% | |
| TATCTTGTATCAGCTGAATT+TGG | - | chr2.4:23852132-23852151 | MS.gene95982:intron | 30.0% | |
| TTGTCTATTATCCAGTGATT+CGG | - | chr2.4:23852042-23852061 | MS.gene95982:CDS | 30.0% | |
| ! | GTAATTGTAGACAAGGTTTT+AGG | - | chr2.4:23851941-23851960 | MS.gene95982:CDS | 30.0% |
| !! | CACTTTAAGACATTGCAATA+TGG | - | chr2.4:23851985-23852004 | MS.gene95982:CDS | 30.0% |
| !! | CTTAAAGTGCTGATTAGTAT+TGG | + | chr2.4:23851974-23851993 | None:intergenic | 30.0% |
| !!! | AATGTTTTGATGGAATCCAA+AGG | - | chr2.4:23852230-23852249 | MS.gene95982:intron | 30.0% |
| AAATCCAAGAAAAACAAGGG+AGG | - | chr2.4:23852332-23852351 | MS.gene95982:CDS | 35.0% | |
| AACTTACCTCATGTTCATAG+AGG | + | chr2.4:23851901-23851920 | None:intergenic | 35.0% | |
| ATCAGCTGAATTTGGCTAAA+AGG | - | chr2.4:23852140-23852159 | MS.gene95982:intron | 35.0% | |
| GAAAAACAAGGGAGGAAAAA+AGG | - | chr2.4:23852340-23852359 | MS.gene95982:CDS | 35.0% | |
| ! | TTTTCGTGTCCTGAATTAAC+CGG | + | chr2.4:23852281-23852300 | None:intergenic | 35.0% |
| !!! | TTTTCCTCCCTTGTTTTTCT+TGG | + | chr2.4:23852339-23852358 | None:intergenic | 35.0% |
| AGGCCAAGGAAAATATCAAG+CGG | - | chr2.4:23852300-23852319 | MS.gene95982:CDS | 40.0% | |
| ATTGTTGGACACGTTACCTT+TGG | + | chr2.4:23852249-23852268 | None:intergenic | 40.0% | |
| GGTTAATTCAGGACACGAAA+AGG | - | chr2.4:23852280-23852299 | MS.gene95982:intron | 40.0% | |
| ! | CCATCAAAACATTTTGCTCG+TGG | + | chr2.4:23852223-23852242 | None:intergenic | 40.0% |
| ! | CTTCCGCTTGATATTTTCCT+TGG | + | chr2.4:23852306-23852325 | None:intergenic | 40.0% |
| !! | CCACGAGCAAAATGTTTTGA+TGG | - | chr2.4:23852220-23852239 | MS.gene95982:intron | 40.0% |
| AACCGGAGCTTGAGAATTGT+TGG | + | chr2.4:23852264-23852283 | None:intergenic | 45.0% | |
| CTTCTCAATCACCGAATCAC+TGG | + | chr2.4:23852056-23852075 | None:intergenic | 45.0% | |
| GTCCAACAATTCTCAAGCTC+CGG | - | chr2.4:23852259-23852278 | MS.gene95982:intron | 45.0% | |
| TCTCAAGCTCCGGTTAATTC+AGG | - | chr2.4:23852269-23852288 | MS.gene95982:intron | 45.0% | |
| TTCGAGCCTCTATGAACATG+AGG | - | chr2.4:23851892-23851911 | MS.gene95982:CDS | 45.0% | |
| ! | TGATGGATCAGACCCAACAT+TGG | + | chr2.4:23852171-23852190 | None:intergenic | 45.0% |
| !! | CAGTGATTCGGTGATTGAGA+AGG | - | chr2.4:23852054-23852073 | MS.gene95982:CDS | 45.0% |
| ATCATTGCCTACTGCAGTGC+TGG | + | chr2.4:23852198-23852217 | None:intergenic | 50.0% | |
| TTCAGGACACGAAAAGGCCA+AGG | - | chr2.4:23852286-23852305 | MS.gene95982:intron | 50.0% | |
| TCACAAGCCAGCACTGCAGT+AGG | - | chr2.4:23852188-23852207 | MS.gene95982:intron | 55.0% | |
| !! | ACTGCAGTGCTGGCTTGTGA+TGG | + | chr2.4:23852188-23852207 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 23851834 | 23852373 | 23851834 | ID=MS.gene95982 |
| chr2.4 | mRNA | 23851834 | 23852373 | 23851834 | ID=MS.gene95982.t1;Parent=MS.gene95982 |
| chr2.4 | exon | 23852294 | 23852373 | 23852294 | ID=MS.gene95982.t1.exon1;Parent=MS.gene95982.t1 |
| chr2.4 | CDS | 23852294 | 23852373 | 23852294 | ID=cds.MS.gene95982.t1;Parent=MS.gene95982.t1 |
| chr2.4 | exon | 23852035 | 23852150 | 23852035 | ID=MS.gene95982.t1.exon2;Parent=MS.gene95982.t1 |
| chr2.4 | CDS | 23852035 | 23852150 | 23852035 | ID=cds.MS.gene95982.t1;Parent=MS.gene95982.t1 |
| chr2.4 | exon | 23851834 | 23852012 | 23851834 | ID=MS.gene95982.t1.exon3;Parent=MS.gene95982.t1 |
| chr2.4 | CDS | 23851834 | 23852012 | 23851834 | ID=cds.MS.gene95982.t1;Parent=MS.gene95982.t1 |
| Gene Sequence |
| Protein sequence |
