AlfalfaGEDB | MS.gene96023

M. sativa cultivar XinJiangDaYe / MS.gene96023

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96023.t1	XP_013464493.1	85.5	200	28	1	1	200	1	199	2.10E-91	345.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96023.t1	P29421	29.1	175	117	2	11	181	6	177	5.8e-13	75.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96023.t1	G8A259	85.5	200	28	1	1	200	1	199	1.5e-91	345.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050584	MS.gene96023	0.815627	8.39E-52	-1.69E-46
MS.gene050585	MS.gene96023	0.817237	3.66E-52	-1.69E-46
MS.gene051789	MS.gene96023	0.815904	7.28E-52	-1.69E-46
MS.gene053074	MS.gene96023	0.836002	1.21E-56	-1.69E-46
MS.gene059006	MS.gene96023	0.836335	9.94E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96023.t1	MTR_2g072980	85.500	200	28	1	1	200	1	199	2.12e-120	339
MS.gene96023.t1	MTR_2g073010	66.829	205	56	4	1	200	1	198	5.67e-87	254
MS.gene96023.t1	MTR_8g059790	46.305	203	85	6	1	188	1	194	7.76e-50	160
MS.gene96023.t1	MTR_0100s0150	44.811	212	92	7	1	199	1	200	1.69e-49	159
MS.gene96023.t1	MTR_0211s0080	44.976	209	93	7	1	199	1	197	2.64e-47	154
MS.gene96023.t1	MTR_0302s0030	42.254	213	97	7	1	199	1	201	8.92e-45	147
MS.gene96023.t1	MTR_8g461050	43.069	202	91	6	1	188	1	192	1.41e-44	147
MS.gene96023.t1	MTR_3g014820	41.589	214	98	7	1	199	1	202	2.63e-44	146
MS.gene96023.t1	MTR_7g108815	43.192	213	94	9	1	199	1	200	5.94e-43	143
MS.gene96023.t1	MTR_3g014870	41.589	214	98	7	1	199	1	202	6.78e-43	143
MS.gene96023.t1	MTR_0084s0030	42.254	213	97	7	1	199	1	201	4.25e-42	140
MS.gene96023.t1	MTR_8g060550	41.709	199	89	8	15	199	14	199	1.72e-40	137
MS.gene96023.t1	MTR_8g461690	41.709	199	89	8	15	199	14	199	1.72e-40	137
MS.gene96023.t1	MTR_6g078070	31.472	197	122	6	14	200	10	203	9.52e-19	80.5
MS.gene96023.t1	MTR_6g078100	31.500	200	120	7	14	200	10	205	6.49e-17	75.9
MS.gene96023.t1	MTR_6g078290	31.707	205	106	8	8	186	4	200	2.21e-13	66.6
MS.gene96023.t1	MTR_6g065460	26.154	195	122	6	10	189	7	194	5.14e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96023.t1	AT1G17860	29.412	204	121	8	11	200	1	195	1.48e-13	66.6

Find 48 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAAATGGTAAGAATTTAT+TGG	0.260927	2.4:+23120680	MS.gene96023:CDS
CTGATCTTATGTAATACTTT+TGG	0.277700	2.4:-23120278	None:intergenic
ACAATCTACAGAATGGAAAT+TGG	0.284846	2.4:+23120491	MS.gene96023:CDS
GTTTCTTGACCCACATATAA+AGG	0.297981	2.4:-23120354	None:intergenic
TGGTAAGAATTTATTGGCTT+TGG	0.310262	2.4:+23120686	MS.gene96023:CDS
GTTGGTAATATGCGAGAAAA+TGG	0.313171	2.4:+23120666	MS.gene96023:CDS
ATACAGTTGTTAAGGTGAAT+AGG	0.332362	2.4:+23120430	MS.gene96023:CDS
CAGAATGGAAATTGGGTGAT+AGG	0.341031	2.4:+23120499	MS.gene96023:CDS
AGATCAGATGCATTCGATTT+CGG	0.343314	2.4:+23120294	MS.gene96023:CDS
CAAGCCTTAGCCTTATGTTA+TGG	0.345426	2.4:+23120175	MS.gene96023:CDS
GGTCAAGAAACTACAGATAT+TGG	0.348677	2.4:+23120366	MS.gene96023:CDS
TACAGTTGTTAAGGTGAATA+GGG	0.357658	2.4:+23120431	MS.gene96023:CDS
AAGACTCCACTTGGAATTGA+AGG	0.410273	2.4:+23120585	MS.gene96023:CDS
AACAGAAGATACAGTTGTTA+AGG	0.418077	2.4:+23120422	MS.gene96023:CDS
CGGTTCATGCCCTTTATATG+TGG	0.431349	2.4:+23120344	MS.gene96023:CDS
CAATCTACAGAATGGAAATT+GGG	0.431621	2.4:+23120492	MS.gene96023:CDS
TTTCTTGACCCACATATAAA+GGG	0.438692	2.4:-23120353	None:intergenic
AATTGGGTGATAGGGACAAT+AGG	0.464287	2.4:+23120508	MS.gene96023:CDS
TTTGATCAATCTTGTTGTCA+TGG	0.481185	2.4:-23120148	None:intergenic
AGGTTGTGCTAATGTTGGTA+TGG	0.501409	2.4:-23120208	None:intergenic
AAGAATTTATTGGCTTTGGA+TGG	0.504929	2.4:+23120690	MS.gene96023:CDS
TCATGAAAAGCCATAACATA+AGG	0.507153	2.4:-23120185	None:intergenic
TTATTGGCTTTGGATGGTAA+TGG	0.511038	2.4:+23120696	MS.gene96023:CDS
ACGGTTAATTGCGGTACGGT+TGG	0.512688	2.4:+23120648	MS.gene96023:CDS
GGAAGGAGGCTTATTACAGC+GGG	0.514020	2.4:+23120534	MS.gene96023:CDS
AGAATGGAAATTGGGTGATA+GGG	0.514805	2.4:+23120500	MS.gene96023:CDS
ATAGGGACAATAGGAGTGGA+AGG	0.516567	2.4:+23120517	MS.gene96023:CDS
GTCACTTTGATAAATAGAAA+CGG	0.522150	2.4:+23120324	MS.gene96023:CDS
CTTATTACAGCGGGGAGTGA+TGG	0.543645	2.4:+23120543	MS.gene96023:CDS
GTTGTGGACAATCTACAGAA+TGG	0.556391	2.4:+23120484	MS.gene96023:CDS
GGTTCATGCCCTTTATATGT+GGG	0.576927	2.4:+23120345	MS.gene96023:CDS
AAAGCCATAACATAAGGCTA+AGG	0.601835	2.4:-23120179	None:intergenic
CCCTCGGATACGGTTAATTG+CGG	0.603924	2.4:+23120639	MS.gene96023:CDS
ACTGTATCTTCTGTTGCCAC+AGG	0.605826	2.4:-23120414	None:intergenic
GGTGATAGGGACAATAGGAG+TGG	0.613024	2.4:+23120513	MS.gene96023:CDS
TTCTATTTATCAAAGTGACA+CGG	0.619288	2.4:-23120320	None:intergenic
TGGAAGGAGGCTTATTACAG+CGG	0.620632	2.4:+23120533	MS.gene96023:CDS
TCAGATGCATTCGATTTCGG+AGG	0.637538	2.4:+23120297	MS.gene96023:CDS
AAAACACCTTCAATTCCAAG+TGG	0.642111	2.4:-23120591	None:intergenic
ACACGCCGTCCTCTTGAGAG+TGG	0.654084	2.4:+23120255	MS.gene96023:CDS
GGATACGGTTAATTGCGGTA+CGG	0.659047	2.4:+23120644	MS.gene96023:CDS
ACCGCAATTAACCGTATCCG+AGG	0.663583	2.4:-23120640	None:intergenic
GAAACTACAGATATTGGACA+AGG	0.666365	2.4:+23120372	MS.gene96023:CDS
TTTGGCCACTCTCAAGAGGA+CGG	0.673647	2.4:-23120260	None:intergenic
GGGACAATAGGAGTGGAAGG+AGG	0.693684	2.4:+23120520	MS.gene96023:CDS
AAACTACAGATATTGGACAA+GGG	0.717751	2.4:+23120373	MS.gene96023:CDS
CCGCAATTAACCGTATCCGA+GGG	0.724427	2.4:-23120639	None:intergenic
GAAGGAGGCTTATTACAGCG+GGG	0.754937	2.4:+23120535	MS.gene96023:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAGGTAATTATAATATT+AGG	+	chr2.4:23120610-23120629	MS.gene96023:CDS	10.0%
!!	AGAAAATGGTAAGAATTTAT+TGG	+	chr2.4:23120680-23120699	MS.gene96023:CDS	20.0%
!	TTCTATTTATCAAAGTGACA+CGG	-	chr2.4:23120323-23120342	None:intergenic	25.0%
!!	CTGATCTTATGTAATACTTT+TGG	-	chr2.4:23120281-23120300	None:intergenic	25.0%
!!	GTCACTTTGATAAATAGAAA+CGG	+	chr2.4:23120324-23120343	MS.gene96023:CDS	25.0%
	AAACTACAGATATTGGACAA+GGG	+	chr2.4:23120373-23120392	MS.gene96023:CDS	30.0%
	AACAGAAGATACAGTTGTTA+AGG	+	chr2.4:23120422-23120441	MS.gene96023:CDS	30.0%
	ACAATCTACAGAATGGAAAT+TGG	+	chr2.4:23120491-23120510	MS.gene96023:CDS	30.0%
	ATACAGTTGTTAAGGTGAAT+AGG	+	chr2.4:23120430-23120449	MS.gene96023:CDS	30.0%
	CAATCTACAGAATGGAAATT+GGG	+	chr2.4:23120492-23120511	MS.gene96023:CDS	30.0%
	TACAGTTGTTAAGGTGAATA+GGG	+	chr2.4:23120431-23120450	MS.gene96023:CDS	30.0%
	TCATGAAAAGCCATAACATA+AGG	-	chr2.4:23120188-23120207	None:intergenic	30.0%
	TTTCTTGACCCACATATAAA+GGG	-	chr2.4:23120356-23120375	None:intergenic	30.0%
!	TAAGGTGAATAGGGATTTTA+AGG	+	chr2.4:23120440-23120459	MS.gene96023:CDS	30.0%
!	TCAAGAACTTCTTCATTTTC+AGG	-	chr2.4:23120231-23120250	None:intergenic	30.0%
!!	AAGAATTTATTGGCTTTGGA+TGG	+	chr2.4:23120690-23120709	MS.gene96023:CDS	30.0%
!!	TAATATTAGGTTTTGTCCCT+CGG	+	chr2.4:23120623-23120642	MS.gene96023:CDS	30.0%
!!	TGGTAAGAATTTATTGGCTT+TGG	+	chr2.4:23120686-23120705	MS.gene96023:CDS	30.0%
!!!	ATCTGTTATTTTTACTCCTG+TGG	+	chr2.4:23120398-23120417	MS.gene96023:CDS	30.0%
!!!	ATGGTAATGGTAATTTTCTC+AGG	+	chr2.4:23120709-23120728	MS.gene96023:CDS	30.0%
!!!	TGGTAATGGTAATTTTCTCA+GGG	+	chr2.4:23120710-23120729	MS.gene96023:CDS	30.0%
	AAAACACCTTCAATTCCAAG+TGG	-	chr2.4:23120594-23120613	None:intergenic	35.0%
	AAAGCCATAACATAAGGCTA+AGG	-	chr2.4:23120182-23120201	None:intergenic	35.0%
	AGAATGGAAATTGGGTGATA+GGG	+	chr2.4:23120500-23120519	MS.gene96023:CDS	35.0%
	AGATCAGATGCATTCGATTT+CGG	+	chr2.4:23120294-23120313	MS.gene96023:CDS	35.0%
	GAAACTACAGATATTGGACA+AGG	+	chr2.4:23120372-23120391	MS.gene96023:CDS	35.0%
	GGTCAAGAAACTACAGATAT+TGG	+	chr2.4:23120366-23120385	MS.gene96023:CDS	35.0%
	GTTTCTTGACCCACATATAA+AGG	-	chr2.4:23120357-23120376	None:intergenic	35.0%
!	AATGGTAATTTTCTCAGGGT+TGG	+	chr2.4:23120714-23120733	MS.gene96023:CDS	35.0%
!	ATGGTAATTTTCTCAGGGTT+GGG	+	chr2.4:23120715-23120734	MS.gene96023:CDS	35.0%
!!	AGAATTTTGAAGACTCCACT+TGG	+	chr2.4:23120576-23120595	MS.gene96023:CDS	35.0%
!!	GTTGGTAATATGCGAGAAAA+TGG	+	chr2.4:23120666-23120685	MS.gene96023:CDS	35.0%
!!	TTATTGGCTTTGGATGGTAA+TGG	+	chr2.4:23120696-23120715	MS.gene96023:CDS	35.0%
!!!	CTTGGAATTGAAGGTGTTTT+AGG	+	chr2.4:23120594-23120613	MS.gene96023:CDS	35.0%
!!!	TTTTCAGCTTCTTCAAGTTG+TGG	+	chr2.4:23120468-23120487	MS.gene96023:CDS	35.0%
!!!	TTTTCAGGTTGTGCTAATGT+TGG	-	chr2.4:23120216-23120235	None:intergenic	35.0%
	AAGACTCCACTTGGAATTGA+AGG	+	chr2.4:23120585-23120604	MS.gene96023:CDS	40.0%
	AATTGGGTGATAGGGACAAT+AGG	+	chr2.4:23120508-23120527	MS.gene96023:CDS	40.0%
	AGGTTGTGCTAATGTTGGTA+TGG	-	chr2.4:23120211-23120230	None:intergenic	40.0%
	CAGAATGGAAATTGGGTGAT+AGG	+	chr2.4:23120499-23120518	MS.gene96023:CDS	40.0%
	GGTTCATGCCCTTTATATGT+GGG	+	chr2.4:23120345-23120364	MS.gene96023:CDS	40.0%
	GTTGTGGACAATCTACAGAA+TGG	+	chr2.4:23120484-23120503	MS.gene96023:CDS	40.0%
!!	CAAGCCTTAGCCTTATGTTA+TGG	+	chr2.4:23120175-23120194	MS.gene96023:CDS	40.0%
	ACTGTATCTTCTGTTGCCAC+AGG	-	chr2.4:23120417-23120436	None:intergenic	45.0%
	ATAGGGACAATAGGAGTGGA+AGG	+	chr2.4:23120517-23120536	MS.gene96023:CDS	45.0%
	CGGTTCATGCCCTTTATATG+TGG	+	chr2.4:23120344-23120363	MS.gene96023:CDS	45.0%
	GGATACGGTTAATTGCGGTA+CGG	+	chr2.4:23120644-23120663	MS.gene96023:CDS	45.0%
	TCAGATGCATTCGATTTCGG+AGG	+	chr2.4:23120297-23120316	MS.gene96023:CDS	45.0%
	TGGAAGGAGGCTTATTACAG+CGG	+	chr2.4:23120533-23120552	MS.gene96023:CDS	45.0%
!	TACTTTTGGCCACTCTCAAG+AGG	-	chr2.4:23120267-23120286	None:intergenic	45.0%
!!	TAGGTTTTGTCCCTCGGATA+CGG	+	chr2.4:23120629-23120648	MS.gene96023:CDS	45.0%
	ACCGCAATTAACCGTATCCG+AGG	-	chr2.4:23120643-23120662	None:intergenic	50.0%
	ACGGTTAATTGCGGTACGGT+TGG	+	chr2.4:23120648-23120667	MS.gene96023:CDS	50.0%
	CCCTCGGATACGGTTAATTG+CGG	+	chr2.4:23120639-23120658	MS.gene96023:CDS	50.0%
	CCGCAATTAACCGTATCCGA+GGG	-	chr2.4:23120642-23120661	None:intergenic	50.0%
	CTTATTACAGCGGGGAGTGA+TGG	+	chr2.4:23120543-23120562	MS.gene96023:CDS	50.0%
	GAAGGAGGCTTATTACAGCG+GGG	+	chr2.4:23120535-23120554	MS.gene96023:CDS	50.0%
	GGAAGGAGGCTTATTACAGC+GGG	+	chr2.4:23120534-23120553	MS.gene96023:CDS	50.0%
	GGTGATAGGGACAATAGGAG+TGG	+	chr2.4:23120513-23120532	MS.gene96023:CDS	50.0%
	TTTGGCCACTCTCAAGAGGA+CGG	-	chr2.4:23120263-23120282	None:intergenic	50.0%
	GGGACAATAGGAGTGGAAGG+AGG	+	chr2.4:23120520-23120539	MS.gene96023:CDS	55.0%
	ACACGCCGTCCTCTTGAGAG+TGG	+	chr2.4:23120255-23120274	MS.gene96023:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	23120150	23120752	23120150	ID=MS.gene96023
chr2.4	mRNA	23120150	23120752	23120150	ID=MS.gene96023.t1;Parent=MS.gene96023
chr2.4	exon	23120150	23120752	23120150	ID=MS.gene96023.t1.exon1;Parent=MS.gene96023.t1
chr2.4	CDS	23120150	23120752	23120150	ID=cds.MS.gene96023.t1;Parent=MS.gene96023.t1

Gene Sequence

>MS.gene96023

ATGACAACAAGATTGATCAAAATCACAAGCCTTAGCCTTATGTTATGGCTTTTCATGACCATACCAACATTAGCACAACCTGAAAATGAAGAAGTTCTTGACTTAACACGCCGTCCTCTTGAGAGTGGCCAAAAGTATTACATAAGATCAGATGCATTCGATTTCGGAGGCCGTGTCACTTTGATAAATAGAAACGGTTCATGCCCTTTATATGTGGGTCAAGAAACTACAGATATTGGACAAGGGTTATCTGTTATTTTTACTCCTGTGGCAACAGAAGATACAGTTGTTAAGGTGAATAGGGATTTTAAGGTGAAATTTTCAGCTTCTTCAAGTTGTGGACAATCTACAGAATGGAAATTGGGTGATAGGGACAATAGGAGTGGAAGGAGGCTTATTACAGCGGGGAGTGATGGTAGTTTATTTAGAATTTTGAAGACTCCACTTGGAATTGAAGGTGTTTTAGGTAATTATAATATTAGGTTTTGTCCCTCGGATACGGTTAATTGCGGTACGGTTGGTAATATGCGAGAAAATGGTAAGAATTTATTGGCTTTGGATGGTAATGGTAATTTTCTCAGGGTTGGGTTTGAGAGAGCATAA

Protein sequence

>MS.gene96023.t1

MTTRLIKITSLSLMLWLFMTIPTLAQPENEEVLDLTRRPLESGQKYYIRSDAFDFGGRVTLINRNGSCPLYVGQETTDIGQGLSVIFTPVATEDTVVKVNRDFKVKFSASSSCGQSTEWKLGDRDNRSGRRLITAGSDGSLFRILKTPLGIEGVLGNYNIRFCPSDTVNCGTVGNMRENGKNLLALDGNGNFLRVGFERA