AlfalfaGEDB | MS.gene96024

M. sativa cultivar XinJiangDaYe / MS.gene96024

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96024.t1	XP_013464493.1	83.4	205	28	1	1	205	1	199	3.70E-91	344.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96024.t1	P29421	30.3	165	111	1	11	171	6	170	2.1e-13	77.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96024.t1	G8A259	83.4	205	28	1	1	205	1	199	2.7e-91	344.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052698	MS.gene96024	0.81397	1.95E-51	-1.69E-46
MS.gene053074	MS.gene96024	0.823449	1.38E-53	-1.69E-46
MS.gene054596	MS.gene96024	0.800332	1.51E-48	-1.69E-46
MS.gene055359	MS.gene96024	0.821554	3.80E-53	-1.69E-46
MS.gene058168	MS.gene96024	0.808473	3.04E-50	-1.69E-46
MS.gene059006	MS.gene96024	0.814319	1.64E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96024.t1	MTR_2g072980	83.415	205	28	1	1	205	1	199	7.04e-121	340
MS.gene96024.t1	MTR_2g073010	66.190	210	54	4	1	205	1	198	1.42e-89	261
MS.gene96024.t1	MTR_8g059790	47.291	203	83	6	1	188	1	194	2.79e-52	167
MS.gene96024.t1	MTR_0211s0080	45.960	198	87	6	1	188	1	188	1.23e-48	157
MS.gene96024.t1	MTR_0100s0150	44.776	201	88	6	1	188	1	191	3.94e-48	156
MS.gene96024.t1	MTR_0302s0030	43.069	202	91	6	1	188	1	192	3.40e-46	151
MS.gene96024.t1	MTR_8g461050	43.564	202	90	6	1	188	1	192	5.19e-46	151
MS.gene96024.t1	MTR_3g014820	42.857	203	91	6	1	188	1	193	5.23e-46	151
MS.gene96024.t1	MTR_3g014870	42.857	203	91	6	1	188	1	193	1.41e-44	147
MS.gene96024.t1	MTR_0084s0030	43.069	202	91	6	1	188	1	192	1.13e-43	145
MS.gene96024.t1	MTR_7g108815	44.059	202	87	8	1	188	1	190	3.12e-43	144
MS.gene96024.t1	MTR_8g060550	40.686	204	89	8	15	204	14	199	1.87e-41	139
MS.gene96024.t1	MTR_8g461690	40.686	204	89	8	15	204	14	199	1.87e-41	139
MS.gene96024.t1	MTR_6g078070	32.727	165	103	5	14	173	10	171	6.49e-18	78.6
MS.gene96024.t1	MTR_6g078100	30.732	205	120	8	14	205	10	205	8.32e-16	72.8
MS.gene96024.t1	MTR_6g078290	30.622	209	109	9	8	189	4	203	1.13e-12	64.7
MS.gene96024.t1	MTR_6g066210	29.050	179	109	7	8	174	4	176	1.06e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96024.t1	AT1G17860	29.187	209	120	8	11	205	1	195	4.65e-14	68.2

Find 50 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGGTAATTTGCATGAAAA+TGG	0.278573	2.4:+23097685	MS.gene96024:CDS
ACAATCTACAGAATGGAAAT+TGG	0.284846	2.4:+23097510	MS.gene96024:CDS
AGTGACACGGCCTCCGATAT+CGG	0.290496	2.4:-23097326	None:intergenic
GTTTCTTGACCCACATATAA+AGG	0.297981	2.4:-23097373	None:intergenic
ATACAGTTGTTAAGGTGAAT+AGG	0.332362	2.4:+23097449	MS.gene96024:CDS
CAGAATGGAAATTGGGTGAT+AGG	0.341031	2.4:+23097518	MS.gene96024:CDS
CAAGCCTTAGCCTTATGTTA+TGG	0.345426	2.4:+23097194	MS.gene96024:CDS
GGTCAAGAAACTACAGATAT+TGG	0.348677	2.4:+23097385	MS.gene96024:CDS
TACAGTTGTTAAGGTGAATA+GGG	0.357658	2.4:+23097450	MS.gene96024:CDS
AAGACTCCACTTGGAATTGA+AGG	0.410273	2.4:+23097604	MS.gene96024:CDS
AAGGTTAATGTTCTCAAGGT+TGG	0.412184	2.4:+23097748	MS.gene96024:CDS
AGGTTAATGTTCTCAAGGTT+GGG	0.412594	2.4:+23097749	MS.gene96024:CDS
AACAGAAGATACAGTTGTTA+AGG	0.418077	2.4:+23097441	MS.gene96024:CDS
CGGTTCATGCCCTTTATATG+TGG	0.431349	2.4:+23097363	MS.gene96024:CDS
CAATCTACAGAATGGAAATT+GGG	0.431621	2.4:+23097511	MS.gene96024:CDS
CTGATCTTATGTAATACTTC+CGG	0.434280	2.4:-23097297	None:intergenic
TTTCTTGACCCACATATAAA+GGG	0.438692	2.4:-23097372	None:intergenic
GTGACACGGCCTCCGATATC+GGG	0.449326	2.4:-23097325	None:intergenic
AGATCAGATGTACCCGATAT+CGG	0.472186	2.4:+23097313	MS.gene96024:CDS
AATTGGGTGATAGGGACAAT+AGG	0.474158	2.4:+23097527	MS.gene96024:CDS
TTTGATCAATCTTGTTGTCA+TGG	0.481185	2.4:-23097167	None:intergenic
AGGTTGTGCTAATGTTGGTA+TGG	0.501409	2.4:-23097227	None:intergenic
TCATGAAAAGCCATAACATA+AGG	0.507153	2.4:-23097204	None:intergenic
AGAATGGAAATTGGGTGATA+GGG	0.514805	2.4:+23097519	MS.gene96024:CDS
GGAAGGAGGCTTATTACAGC+GGG	0.521625	2.4:+23097553	MS.gene96024:CDS
GTCACTTTGATAAATAGAAA+CGG	0.522150	2.4:+23097343	MS.gene96024:CDS
GTCGTCCTCTTGAGAGTGGC+CGG	0.536921	2.4:+23097278	MS.gene96024:CDS
CTTATTACAGCGGGGAGTGA+TGG	0.543645	2.4:+23097562	MS.gene96024:CDS
TGGTATTAATAATGTTCTCA+AGG	0.548464	2.4:+23097729	MS.gene96024:CDS
ATTGTGGACAATCTACAGAA+TGG	0.552740	2.4:+23097503	MS.gene96024:CDS
TCTCAAGGTTAATGTTCTCA+AGG	0.553729	2.4:+23097744	MS.gene96024:CDS
ACGGTTAATTGCGGTACTGT+TGG	0.557530	2.4:+23097667	MS.gene96024:CDS
ACTGTATCTTCTGTTGCCAT+AGG	0.571927	2.4:-23097433	None:intergenic
GGTTCATGCCCTTTATATGT+GGG	0.576927	2.4:+23097364	MS.gene96024:CDS
ACCGCAATTAACCGTTTCCG+AGG	0.585521	2.4:-23097659	None:intergenic
CGGAAGGAGGCTTATTACAG+CGG	0.595232	2.4:+23097552	MS.gene96024:CDS
ATAGGGACAATAGGAGCGGA+AGG	0.600182	2.4:+23097536	MS.gene96024:CDS
AAAGCCATAACATAAGGCTA+AGG	0.601835	2.4:-23097198	None:intergenic
TACTTCCGGCCACTCTCAAG+AGG	0.616975	2.4:-23097283	None:intergenic
TTCTATTTATCAAAGTGACA+CGG	0.619288	2.4:-23097339	None:intergenic
ACACGTCGTCCTCTTGAGAG+TGG	0.635863	2.4:+23097274	MS.gene96024:CDS
AAAACACCTTCAATTCCAAG+TGG	0.642111	2.4:-23097610	None:intergenic
CCGCAATTAACCGTTTCCGA+GGG	0.650030	2.4:-23097658	None:intergenic
CCCTCGGAAACGGTTAATTG+CGG	0.663767	2.4:+23097658	MS.gene96024:CDS
GAAACTACAGATATTGGACA+AGG	0.666365	2.4:+23097391	MS.gene96024:CDS
GGGACAATAGGAGCGGAAGG+AGG	0.666908	2.4:+23097539	MS.gene96024:CDS
GGTGATAGGGACAATAGGAG+CGG	0.692836	2.4:+23097532	MS.gene96024:CDS
TCAGATGTACCCGATATCGG+AGG	0.696816	2.4:+23097316	MS.gene96024:CDS
AAACTACAGATATTGGACAA+GGG	0.717751	2.4:+23097392	MS.gene96024:CDS
GAAGGAGGCTTATTACAGCG+GGG	0.754937	2.4:+23097554	MS.gene96024:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAGGTAATTATAATATT+AGG	+	chr2.4:23097629-23097648	MS.gene96024:CDS	10.0%
!!!	TGAAAATGGTAAGATTTTAT+TGG	+	chr2.4:23097699-23097718	MS.gene96024:CDS	20.0%
!	TGGTATTAATAATGTTCTCA+AGG	+	chr2.4:23097729-23097748	MS.gene96024:CDS	25.0%
!	TTCTATTTATCAAAGTGACA+CGG	-	chr2.4:23097342-23097361	None:intergenic	25.0%
!!	GTCACTTTGATAAATAGAAA+CGG	+	chr2.4:23097343-23097362	MS.gene96024:CDS	25.0%
!!!	ATCAGTTATTTTTACTCCTA+TGG	+	chr2.4:23097417-23097436	MS.gene96024:CDS	25.0%
	AAACTACAGATATTGGACAA+GGG	+	chr2.4:23097392-23097411	MS.gene96024:CDS	30.0%
	AACAGAAGATACAGTTGTTA+AGG	+	chr2.4:23097441-23097460	MS.gene96024:CDS	30.0%
	ACAATCTACAGAATGGAAAT+TGG	+	chr2.4:23097510-23097529	MS.gene96024:CDS	30.0%
	ATACAGTTGTTAAGGTGAAT+AGG	+	chr2.4:23097449-23097468	MS.gene96024:CDS	30.0%
	CAATCTACAGAATGGAAATT+GGG	+	chr2.4:23097511-23097530	MS.gene96024:CDS	30.0%
	CTGATCTTATGTAATACTTC+CGG	-	chr2.4:23097300-23097319	None:intergenic	30.0%
	TACAGTTGTTAAGGTGAATA+GGG	+	chr2.4:23097450-23097469	MS.gene96024:CDS	30.0%
	TCATGAAAAGCCATAACATA+AGG	-	chr2.4:23097207-23097226	None:intergenic	30.0%
	TTTCTTGACCCACATATAAA+GGG	-	chr2.4:23097375-23097394	None:intergenic	30.0%
!	TAAGGTGAATAGGGATTTTA+AGG	+	chr2.4:23097459-23097478	MS.gene96024:CDS	30.0%
!	TCAAGAACTTCTTCATTTTC+AGG	-	chr2.4:23097250-23097269	None:intergenic	30.0%
!	TTTTCAGCTTCTTCAAATTG+TGG	+	chr2.4:23097487-23097506	MS.gene96024:CDS	30.0%
!!	GTTGGTAATTTGCATGAAAA+TGG	+	chr2.4:23097685-23097704	MS.gene96024:CDS	30.0%
!!	TAATATTAGGTTTTGTCCCT+CGG	+	chr2.4:23097642-23097661	MS.gene96024:CDS	30.0%
!!!	AAGATTTTATTGGCTTTGGA+TGG	+	chr2.4:23097709-23097728	MS.gene96024:CDS	30.0%
!!!	TGGTAAGATTTTATTGGCTT+TGG	+	chr2.4:23097705-23097724	MS.gene96024:CDS	30.0%
	AAAACACCTTCAATTCCAAG+TGG	-	chr2.4:23097613-23097632	None:intergenic	35.0%
	AAAGCCATAACATAAGGCTA+AGG	-	chr2.4:23097201-23097220	None:intergenic	35.0%
	AAGGTTAATGTTCTCAAGGT+TGG	+	chr2.4:23097748-23097767	MS.gene96024:CDS	35.0%
	AGAATGGAAATTGGGTGATA+GGG	+	chr2.4:23097519-23097538	MS.gene96024:CDS	35.0%
	AGGTTAATGTTCTCAAGGTT+GGG	+	chr2.4:23097749-23097768	MS.gene96024:CDS	35.0%
	ATTGTGGACAATCTACAGAA+TGG	+	chr2.4:23097503-23097522	MS.gene96024:CDS	35.0%
	GAAACTACAGATATTGGACA+AGG	+	chr2.4:23097391-23097410	MS.gene96024:CDS	35.0%
	GGTCAAGAAACTACAGATAT+TGG	+	chr2.4:23097385-23097404	MS.gene96024:CDS	35.0%
	GTTTCTTGACCCACATATAA+AGG	-	chr2.4:23097376-23097395	None:intergenic	35.0%
	TCTCAAGGTTAATGTTCTCA+AGG	+	chr2.4:23097744-23097763	MS.gene96024:CDS	35.0%
!!	AGAATTTTGAAGACTCCACT+TGG	+	chr2.4:23097595-23097614	MS.gene96024:CDS	35.0%
!!!	CTTGGAATTGAAGGTGTTTT+AGG	+	chr2.4:23097613-23097632	MS.gene96024:CDS	35.0%
!!!	TTTTCAGGTTGTGCTAATGT+TGG	-	chr2.4:23097235-23097254	None:intergenic	35.0%
	AAGACTCCACTTGGAATTGA+AGG	+	chr2.4:23097604-23097623	MS.gene96024:CDS	40.0%
	AATTGGGTGATAGGGACAAT+AGG	+	chr2.4:23097527-23097546	MS.gene96024:CDS	40.0%
	ACTGTATCTTCTGTTGCCAT+AGG	-	chr2.4:23097436-23097455	None:intergenic	40.0%
	AGATCAGATGTACCCGATAT+CGG	+	chr2.4:23097313-23097332	MS.gene96024:CDS	40.0%
	AGGTTGTGCTAATGTTGGTA+TGG	-	chr2.4:23097230-23097249	None:intergenic	40.0%
	CAGAATGGAAATTGGGTGAT+AGG	+	chr2.4:23097518-23097537	MS.gene96024:CDS	40.0%
	GGTTCATGCCCTTTATATGT+GGG	+	chr2.4:23097364-23097383	MS.gene96024:CDS	40.0%
!!	CAAGCCTTAGCCTTATGTTA+TGG	+	chr2.4:23097194-23097213	MS.gene96024:CDS	40.0%
	ACGGTTAATTGCGGTACTGT+TGG	+	chr2.4:23097667-23097686	MS.gene96024:CDS	45.0%
	CGGTTCATGCCCTTTATATG+TGG	+	chr2.4:23097363-23097382	MS.gene96024:CDS	45.0%
!!	TAGGTTTTGTCCCTCGGAAA+CGG	+	chr2.4:23097648-23097667	MS.gene96024:CDS	45.0%
	ACCGCAATTAACCGTTTCCG+AGG	-	chr2.4:23097662-23097681	None:intergenic	50.0%
	ATAGGGACAATAGGAGCGGA+AGG	+	chr2.4:23097536-23097555	MS.gene96024:CDS	50.0%
	CCCTCGGAAACGGTTAATTG+CGG	+	chr2.4:23097658-23097677	MS.gene96024:CDS	50.0%
	CCGCAATTAACCGTTTCCGA+GGG	-	chr2.4:23097661-23097680	None:intergenic	50.0%
	CGGAAGGAGGCTTATTACAG+CGG	+	chr2.4:23097552-23097571	MS.gene96024:CDS	50.0%
	CTTATTACAGCGGGGAGTGA+TGG	+	chr2.4:23097562-23097581	MS.gene96024:CDS	50.0%
	GAAGGAGGCTTATTACAGCG+GGG	+	chr2.4:23097554-23097573	MS.gene96024:CDS	50.0%
	GGAAGGAGGCTTATTACAGC+GGG	+	chr2.4:23097553-23097572	MS.gene96024:CDS	50.0%
	GGTGATAGGGACAATAGGAG+CGG	+	chr2.4:23097532-23097551	MS.gene96024:CDS	50.0%
	TCAGATGTACCCGATATCGG+AGG	+	chr2.4:23097316-23097335	MS.gene96024:CDS	50.0%
	ACACGTCGTCCTCTTGAGAG+TGG	+	chr2.4:23097274-23097293	MS.gene96024:CDS	55.0%
	AGTGACACGGCCTCCGATAT+CGG	-	chr2.4:23097329-23097348	None:intergenic	55.0%
	TACTTCCGGCCACTCTCAAG+AGG	-	chr2.4:23097286-23097305	None:intergenic	55.0%
	GGGACAATAGGAGCGGAAGG+AGG	+	chr2.4:23097539-23097558	MS.gene96024:CDS	60.0%
	GTCGTCCTCTTGAGAGTGGC+CGG	+	chr2.4:23097278-23097297	MS.gene96024:CDS	60.0%
	GTGACACGGCCTCCGATATC+GGG	-	chr2.4:23097328-23097347	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	23097169	23097786	23097169	ID=MS.gene96024
chr2.4	mRNA	23097169	23097786	23097169	ID=MS.gene96024.t1;Parent=MS.gene96024
chr2.4	exon	23097169	23097786	23097169	ID=MS.gene96024.t1.exon1;Parent=MS.gene96024.t1
chr2.4	CDS	23097169	23097786	23097169	ID=cds.MS.gene96024.t1;Parent=MS.gene96024.t1

Gene Sequence

>MS.gene96024

ATGACAACAAGATTGATCAAAATCACAAGCCTTAGCCTTATGTTATGGCTTTTCATGACCATACCAACATTAGCACAACCTGAAAATGAAGAAGTTCTTGACTTAACACGTCGTCCTCTTGAGAGTGGCCGGAAGTATTACATAAGATCAGATGTACCCGATATCGGAGGCCGTGTCACTTTGATAAATAGAAACGGTTCATGCCCTTTATATGTGGGTCAAGAAACTACAGATATTGGACAAGGGTTATCAGTTATTTTTACTCCTATGGCAACAGAAGATACAGTTGTTAAGGTGAATAGGGATTTTAAGGTGAAATTTTCAGCTTCTTCAAATTGTGGACAATCTACAGAATGGAAATTGGGTGATAGGGACAATAGGAGCGGAAGGAGGCTTATTACAGCGGGGAGTGATGGTAGTTATTTTAGAATTTTGAAGACTCCACTTGGAATTGAAGGTGTTTTAGGTAATTATAATATTAGGTTTTGTCCCTCGGAAACGGTTAATTGCGGTACTGTTGGTAATTTGCATGAAAATGGTAAGATTTTATTGGCTTTGGATGGTATTAATAATGTTCTCAAGGTTAATGTTCTCAAGGTTGGGTTCGAGAGAGCGTAA

Protein sequence

>MS.gene96024.t1

MTTRLIKITSLSLMLWLFMTIPTLAQPENEEVLDLTRRPLESGRKYYIRSDVPDIGGRVTLINRNGSCPLYVGQETTDIGQGLSVIFTPMATEDTVVKVNRDFKVKFSASSNCGQSTEWKLGDRDNRSGRRLITAGSDGSYFRILKTPLGIEGVLGNYNIRFCPSETVNCGTVGNLHENGKILLALDGINNVLKVNVLKVGFERA