AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96101

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96101.t1 MTR_7g074935 100.000 117 0 0 41 157 120 236 6.98e-78 234
MS.gene96101.t1 MTR_7g074935 95.238 42 2 0 1 42 1 42 6.75e-21 87.4
MS.gene96101.t1 MTR_8g036160 89.076 119 12 1 40 157 134 252 7.49e-60 189
MS.gene96101.t1 MTR_4g101990 87.069 116 14 1 43 157 139 254 5.68e-48 158
MS.gene96101.t1 MTR_1g076710 77.391 115 25 1 43 157 129 242 8.43e-48 157
MS.gene96101.t1 MTR_1g061530 64.286 112 39 1 47 157 140 251 3.25e-45 151
MS.gene96101.t1 MTR_6g061670 65.789 114 39 0 44 157 121 234 1.17e-43 146
MS.gene96101.t1 MTR_6g061670 65.789 114 39 0 44 157 126 239 1.52e-43 146
MS.gene96101.t1 MTR_5g011680 60.833 120 43 1 41 156 135 254 2.36e-40 137
MS.gene96101.t1 MTR_8g031100 61.739 115 44 0 43 157 150 264 3.91e-38 133
MS.gene96101.t1 MTR_5g010330 56.522 115 49 1 43 157 129 242 1.09e-37 131
MS.gene96101.t1 MTR_8g098390 55.963 109 48 0 43 151 161 269 2.42e-36 128
MS.gene96101.t1 MTR_4g098460 57.522 113 47 1 45 157 78 189 1.15e-35 125
MS.gene96101.t1 MTR_7g077120 55.652 115 51 0 43 157 152 266 2.41e-35 125
MS.gene96101.t1 MTR_5g011620 58.594 128 48 1 29 156 96 218 7.00e-35 128
MS.gene96101.t1 MTR_4g098460 55.085 118 47 2 45 157 78 194 3.31e-33 119
MS.gene96101.t1 MTR_5g011610 54.198 131 53 2 29 157 37 162 3.92e-33 119
MS.gene96101.t1 MTR_5g011610 55.000 100 42 1 43 142 207 303 3.37e-27 103
MS.gene96101.t1 MTR_4g055803 53.333 120 53 2 41 157 118 237 4.41e-33 121
MS.gene96101.t1 MTR_4g055813 55.963 109 45 2 50 157 21 127 5.35e-33 118
MS.gene96101.t1 MTR_4g055833 51.240 121 56 2 40 157 114 234 6.58e-32 116
MS.gene96101.t1 MTR_1g079780 54.054 111 47 2 48 157 109 216 2.08e-30 112
MS.gene96101.t1 MTR_7g116320 54.783 115 49 1 43 157 138 249 5.48e-28 105
MS.gene96101.t1 MTR_7g116320 54.783 115 49 1 43 157 138 249 2.47e-27 105
MS.gene96101.t1 MTR_5g041430 44.538 119 60 3 42 157 105 220 1.87e-24 96.7
MS.gene96101.t1 MTR_4g055705 44.828 116 61 2 45 157 134 249 4.24e-24 95.5
MS.gene96101.t1 MTR_5g014360 44.444 126 66 2 33 157 175 297 8.77e-24 95.5
MS.gene96101.t1 MTR_1g038960 49.123 114 54 2 45 157 147 257 2.57e-23 94.0
MS.gene96101.t1 MTR_3g023560 39.683 126 70 2 35 157 49 171 3.12e-21 87.0
MS.gene96101.t1 MTR_3g100470 40.000 105 62 1 50 153 103 207 9.52e-21 86.3
MS.gene96101.t1 MTR_1g038990 44.545 110 57 2 45 153 128 234 1.68e-19 83.2
MS.gene96101.t1 MTR_3g068035 44.340 106 56 1 51 156 109 211 1.91e-19 83.2
MS.gene96101.t1 MTR_5g066020 40.566 106 59 3 50 153 82 185 2.67e-19 81.6
MS.gene96101.t1 MTR_5g013390 40.351 114 64 2 45 157 145 255 7.69e-19 81.6
MS.gene96101.t1 MTR_6g060670 45.690 116 58 3 42 157 171 281 1.77e-18 80.5
MS.gene96101.t1 MTR_7g080980 52.239 67 32 0 51 117 87 153 5.74e-18 78.6
MS.gene96101.t1 MTR_4g098450 50.746 67 33 0 51 117 106 172 2.05e-17 77.4
MS.gene96101.t1 MTR_8g036060 54.545 66 30 0 51 116 116 181 8.72e-17 75.9
MS.gene96101.t1 MTR_3g116100 42.727 110 57 3 48 157 145 248 1.09e-16 75.9
MS.gene96101.t1 MTR_5g011700 45.000 100 51 2 59 157 10 106 1.68e-16 73.2
MS.gene96101.t1 MTR_1g073860 47.761 67 35 0 51 117 124 190 1.72e-16 75.1
MS.gene96101.t1 MTR_5g091630 47.761 67 35 0 51 117 77 143 1.80e-16 74.3
MS.gene96101.t1 MTR_1g044155 44.776 67 37 0 51 117 156 222 8.15e-16 73.6
MS.gene96101.t1 MTR_1g079810 47.761 67 35 0 51 117 104 170 1.24e-15 72.4
MS.gene96101.t1 MTR_5g011520 44.340 106 56 1 51 156 130 232 1.80e-15 72.4
MS.gene96101.t1 MTR_5g080580 40.000 105 60 1 52 156 120 221 9.93e-13 64.7
MS.gene96101.t1 MTR_5g011590 50.769 65 30 1 41 105 77 139 3.64e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96101.t1 AT4G12080 86.087 115 16 0 43 157 165 279 6.52e-66 204
MS.gene96101.t1 AT4G25320 72.881 118 32 0 40 157 158 275 1.73e-55 179
MS.gene96101.t1 AT4G00200 84.821 112 17 0 46 157 121 232 4.19e-53 171
MS.gene96101.t1 AT4G00200 84.821 112 17 0 46 157 121 232 4.19e-53 171
MS.gene96101.t1 AT5G62260 71.053 114 33 0 44 157 156 269 1.02e-52 169
MS.gene96101.t1 AT5G62260 71.053 114 33 0 44 157 156 269 1.03e-51 169
MS.gene96101.t1 AT5G62260 71.053 114 33 0 44 157 156 269 1.03e-51 169
MS.gene96101.t1 AT4G22770 79.661 118 24 0 40 157 142 259 1.09e-51 167
MS.gene96101.t1 AT4G22770 79.661 118 24 0 40 157 142 259 1.09e-51 167
MS.gene96101.t1 AT5G51590 71.930 114 32 0 44 157 174 287 1.79e-51 169
MS.gene96101.t1 AT2G33620 66.957 115 38 0 43 157 158 272 1.01e-48 160
MS.gene96101.t1 AT2G33620 66.957 115 38 0 43 157 158 272 1.01e-48 160
MS.gene96101.t1 AT2G33620 66.957 115 38 0 43 157 158 272 1.01e-48 160
MS.gene96101.t1 AT2G33620 66.957 115 38 0 43 157 158 272 1.01e-48 160
MS.gene96101.t1 AT2G45850 60.000 115 46 0 43 157 157 271 2.73e-42 142
MS.gene96101.t1 AT2G45850 60.000 115 46 0 43 157 157 271 6.37e-42 143
MS.gene96101.t1 AT2G45850 60.000 115 46 0 43 157 157 271 6.37e-42 143
MS.gene96101.t1 AT1G63470 57.391 115 48 1 43 157 172 285 1.97e-39 137
MS.gene96101.t1 AT4G17950 54.622 119 52 1 41 157 214 332 1.61e-37 133
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 149 262 5.07e-37 130
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 149 262 5.07e-37 130
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 149 262 5.07e-37 130
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 149 262 5.07e-37 130
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 149 262 5.07e-37 130
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 155 268 5.11e-37 130
MS.gene96101.t1 AT1G63480 56.522 115 49 1 43 157 155 268 5.11e-37 130
MS.gene96101.t1 AT3G61310 58.261 115 48 0 43 157 159 273 8.31e-35 124
MS.gene96101.t1 AT3G04590 52.212 113 51 1 45 157 168 277 1.25e-34 123
MS.gene96101.t1 AT3G04590 51.754 114 52 1 44 157 167 277 1.14e-33 122
MS.gene96101.t1 AT5G46640 58.120 117 49 0 41 157 173 289 6.35e-32 117
MS.gene96101.t1 AT2G36560 40.000 115 67 2 45 157 102 216 1.79e-20 87.4
MS.gene96101.t1 AT5G49700 38.889 108 62 2 50 153 85 192 2.34e-19 82.4
MS.gene96101.t1 AT3G60870 39.450 109 61 2 48 156 86 189 1.19e-18 80.1
MS.gene96101.t1 AT1G14490 40.741 108 60 2 50 153 32 139 7.77e-17 74.3
MS.gene96101.t1 AT1G14490 40.741 108 60 2 50 153 51 158 9.84e-17 74.3
MS.gene96101.t1 AT3G55560 42.453 106 59 1 51 156 118 221 4.77e-16 73.9
MS.gene96101.t1 AT4G14465 43.284 67 38 0 51 117 97 163 8.75e-16 72.8
MS.gene96101.t1 AT4G17800 47.761 67 35 0 51 117 112 178 1.00e-15 72.8
MS.gene96101.t1 AT5G28590 37.857 140 61 3 18 157 2 115 1.26e-15 71.2
MS.gene96101.t1 AT2G42940 49.231 65 33 0 53 117 85 149 1.98e-15 71.6
MS.gene96101.t1 AT2G35270 44.776 67 37 0 51 117 108 174 3.58e-15 71.2
MS.gene96101.t1 AT1G76500 36.522 115 61 2 51 156 102 213 1.95e-14 69.3
MS.gene96101.t1 AT3G04570 41.333 75 36 1 51 117 110 184 1.12e-12 64.3
MS.gene96101.t1 AT4G22810 42.029 69 37 1 51 116 135 203 1.52e-12 64.3
MS.gene96101.t1 AT2G45430 45.714 70 34 1 51 116 119 188 1.76e-12 63.9
MS.gene96101.t1 AT4G12050 43.284 67 37 1 51 116 148 214 2.89e-12 63.5
MS.gene96101.t1 AT4G35390 39.815 108 60 2 51 156 93 197 6.81e-11 59.3

Find 50 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTCAGCCTGATTCTTC+TGG 0.325349 7.4:+30219904 MS.gene96101:CDS
GTCGTAGGCGGTGGAGTTGC+CGG 0.328102 7.4:+30220347 MS.gene96101:CDS
CATGTAACTGTTCCTGAATC+TGG 0.348264 7.4:+30219158 MS.gene96101:CDS
CAGACCGGCTTCTTGTTCCT+TGG 0.373154 7.4:-30220286 None:intergenic
TGGGTTGGAGCTTCACTCCT+TGG 0.378803 7.4:-30219134 None:intergenic
TTTGAGATACTTTCACTGTC+TGG 0.395298 7.4:+30220242 MS.gene96101:CDS
AGAGCCACATTACCAGATTC+AGG 0.396775 7.4:-30219170 None:intergenic
GCATACCTCGCCTGCATTGA+CGG 0.421805 7.4:-30219683 None:intergenic
GCTTCTTGTTCCTTGGTTGT+CGG 0.436064 7.4:-30220279 None:intergenic
TTCAGGAACAGTTACATGTT+GGG 0.439234 7.4:-30219153 None:intergenic
AAAGTTCCGCCAGAAGAATC+AGG 0.448010 7.4:-30219913 None:intergenic
AGCTCCATCAACTTATCATA+TGG 0.455470 7.4:+30219109 MS.gene96101:CDS
CAAGGAACAAGAAGCCGGTC+TGG 0.455999 7.4:+30220287 MS.gene96101:CDS
TGTAGCTGCCACTCCTGTGC+AGG 0.467741 7.4:+30220376 MS.gene96101:CDS
TAGTTTGATATTTGCATGGA+GGG 0.470634 7.4:+30219044 None:intergenic
TCGCGGTCCTTGTTGCGAAA+AGG 0.476324 7.4:-30219836 None:intergenic
GTTTCAGGACATTACAATGA+AGG 0.478893 7.4:+30219807 MS.gene96101:intron
GATGGTCGTGTCGTAGGCGG+TGG 0.481762 7.4:+30220338 MS.gene96101:CDS
AAATCACGCCACTAGCAGAT+AGG 0.488464 7.4:-30219870 None:intergenic
CTTGTTCCTTGGTTGTCGGT+AGG 0.489709 7.4:-30220275 None:intergenic
AACACTGGAGTAACAGTGAT+AGG 0.495781 7.4:+30219080 MS.gene96101:CDS
TGGCGGAACTTTGACCTATG+AGG 0.508265 7.4:+30219924 MS.gene96101:CDS
CATATCATTACCGTCAATGC+AGG 0.510031 7.4:+30219673 MS.gene96101:CDS
TGAGAAATATTTACCTGCAC+AGG 0.521667 7.4:-30220389 None:intergenic
AATGATATGCGGCATAAAGT+TGG 0.521723 7.4:-30219659 None:intergenic
AAGAACCAGAATCACCTCAT+AGG 0.526234 7.4:-30219938 None:intergenic
GGAACAGTTACATGTTGGGT+TGG 0.529114 7.4:-30219149 None:intergenic
TGTTACTTCTGAAGGTGACT+CGG 0.530259 7.4:+30219630 MS.gene96101:intron
AGTCCTGATGGTCGTGTCGT+AGG 0.531638 7.4:+30220332 MS.gene96101:CDS
CCGCCTACGACACGACCATC+AGG 0.536498 7.4:-30220335 None:intergenic
ATTTGCATGGAGGGGAGAGA+AGG 0.539913 7.4:+30219053 None:intergenic
ATTCAGGAACAGTTACATGT+TGG 0.551299 7.4:-30219154 None:intergenic
GTTTCTTTGTCAAGTCCTGA+TGG 0.553718 7.4:+30220320 MS.gene96101:CDS
CGTCAGCCTGATTCTTCTGG+CGG 0.554405 7.4:+30219907 MS.gene96101:CDS
GGTGCCATATGATAAGTTGA+TGG 0.559304 7.4:-30219113 None:intergenic
GGAACAAGAAGCCGGTCTGG+TGG 0.561728 7.4:+30220290 MS.gene96101:CDS
AGTTTGATATTTGCATGGAG+GGG 0.566173 7.4:+30219045 None:intergenic
AGTGGCAGCTACAAGAAGAC+CGG 0.591724 7.4:-30220366 None:intergenic
AATATTTACCTGCACAGGAG+TGG 0.592423 7.4:-30220384 None:intergenic
CCTGATGGTCGTGTCGTAGG+CGG 0.605547 7.4:+30220335 MS.gene96101:CDS
AAACACTCATTCCACCAGAC+CGG 0.616190 7.4:-30220301 None:intergenic
GGGAGAGAAGGTAACAACAC+TGG 0.626647 7.4:+30219065 MS.gene96101:CDS
ACAACCAAGGAACAAGAAGC+CGG 0.627528 7.4:+30220282 MS.gene96101:CDS
ATATGCATCCTATCTGCTAG+TGG 0.629472 7.4:+30219862 MS.gene96101:CDS
ACTTCTGAAGGTGACTCGGT+CGG 0.637545 7.4:+30219634 MS.gene96101:intron
CATTACCGTCAATGCAGGCG+AGG 0.657493 7.4:+30219678 MS.gene96101:CDS
GCATTGACGGTAATGATATG+CGG 0.675288 7.4:-30219670 None:intergenic
CAACTTATCATATGGCACCA+AGG 0.682724 7.4:+30219117 MS.gene96101:CDS
GATAGGATGCATATAGCTCG+CGG 0.699011 7.4:-30219853 None:intergenic
TTCATGCCTACCGACAACCA+AGG 0.742195 7.4:+30220269 MS.gene96101:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGTACATCTTTAATAT+CGG + chr7.4:30219984-30220003 MS.gene96101:intron 15.0%
!!! TAATATTGGTCTATAAAAAA+AGG - chr7.4:30219458-30219477 None:intergenic 15.0%
!!! TTTTCATTTTCATTGATTAT+TGG + chr7.4:30220160-30220179 MS.gene96101:intron 15.0%
!! AACTACTAACTTACTAATAT+TGG - chr7.4:30219472-30219491 None:intergenic 20.0%
!! ATCTAATGTTAATTCTTCTT+TGG + chr7.4:30219741-30219760 MS.gene96101:intron 20.0%
!! GATGAAAATTCATTAGAAAA+AGG - chr7.4:30219329-30219348 None:intergenic 20.0%
!! TCAATGAAAATGAAAAACTA+TGG - chr7.4:30220156-30220175 None:intergenic 20.0%
!! TCTAATGTTAATTCTTCTTT+GGG + chr7.4:30219742-30219761 MS.gene96101:intron 20.0%
!!! ACATAATTTTGTTGTATACT+AGG - chr7.4:30219595-30219614 None:intergenic 20.0%
!!! TTTTCTAATGAATTTTCATC+CGG + chr7.4:30219329-30219348 MS.gene96101:intron 20.0%
! AGGAACAATTAAGAAGAAAA+GGG + chr7.4:30219226-30219245 MS.gene96101:intron 25.0%
! ATCAAATCAAAAATACCTAG+AGG - chr7.4:30219413-30219432 None:intergenic 25.0%
!! TTTCTAATGAATTTTCATCC+GGG + chr7.4:30219330-30219349 MS.gene96101:intron 25.0%
!!! AGTAGTTAAGTTAGTTTTCA+GGG + chr7.4:30219485-30219504 MS.gene96101:intron 25.0%
!!! GGTATGAATTTTGTTTTGAT+TGG + chr7.4:30220181-30220200 MS.gene96101:intron 25.0%
!!! TAGTAGTTAAGTTAGTTTTC+AGG + chr7.4:30219484-30219503 MS.gene96101:intron 25.0%
ATGAATGTTGAACTTATACG+AGG - chr7.4:30220126-30220145 None:intergenic 30.0%
GAAGTAACAACAATAGTTAG+TGG - chr7.4:30219619-30219638 None:intergenic 30.0%
GAGGAACAATTAAGAAGAAA+AGG + chr7.4:30219225-30219244 MS.gene96101:intron 30.0%
GGAACAATTAAGAAGAAAAG+GGG + chr7.4:30219227-30219246 MS.gene96101:intron 30.0%
GTATAAGTTCAACATTCATG+TGG + chr7.4:30220127-30220146 MS.gene96101:intron 30.0%
! ACTATTGTTGTTACTTCTGA+AGG + chr7.4:30219622-30219641 MS.gene96101:intron 30.0%
! ATGTTTCATTTTATCCCCTA+GGG + chr7.4:30220215-30220234 MS.gene96101:intron 30.0%
!!! CTTTACTCTTTTGTTGTTTC+AGG + chr7.4:30219792-30219811 MS.gene96101:intron 30.0%
AAACCTCTGTCACACAAAAT+AGG + chr7.4:30220021-30220040 MS.gene96101:intron 35.0%
AACTTCAAGCAACATTCATG+TGG + chr7.4:30220075-30220094 MS.gene96101:intron 35.0%
AATGATATGCGGCATAAAGT+TGG - chr7.4:30219662-30219681 None:intergenic 35.0%
ACACAAAATAGGACTTCGTT+CGG + chr7.4:30220032-30220051 MS.gene96101:intron 35.0%
AGCTCCATCAACTTATCATA+TGG + chr7.4:30219109-30219128 MS.gene96101:CDS 35.0%
ATTCAGGAACAGTTACATGT+TGG - chr7.4:30219157-30219176 None:intergenic 35.0%
GTTTCAGGACATTACAATGA+AGG + chr7.4:30219807-30219826 MS.gene96101:intron 35.0%
TTCAAGCAGTCAAAAAAAGC+TGG + chr7.4:30219380-30219399 MS.gene96101:intron 35.0%
TTCAGGAACAGTTACATGTT+GGG - chr7.4:30219156-30219175 None:intergenic 35.0%
! CATGTTTCATTTTATCCCCT+AGG + chr7.4:30220214-30220233 MS.gene96101:intron 35.0%
! GTTATATCCTTTTCGCAACA+AGG + chr7.4:30219829-30219848 MS.gene96101:CDS 35.0%
! TTTGAGATACTTTCACTGTC+TGG + chr7.4:30220242-30220261 MS.gene96101:CDS 35.0%
!! ACACTAAAAGTGTTTCAAGG+AGG - chr7.4:30219524-30219543 None:intergenic 35.0%
!! CACTAAAAGTGTTTCAAGGA+GGG - chr7.4:30219523-30219542 None:intergenic 35.0%
AACACTGGAGTAACAGTGAT+AGG + chr7.4:30219080-30219099 MS.gene96101:CDS 40.0%
AAGAACCAGAATCACCTCAT+AGG - chr7.4:30219941-30219960 None:intergenic 40.0%
AAGTATCTCAAATCGACCCT+AGG - chr7.4:30220234-30220253 None:intergenic 40.0%
AGTATCTCAAATCGACCCTA+GGG - chr7.4:30220233-30220252 None:intergenic 40.0%
ATATGCATCCTATCTGCTAG+TGG + chr7.4:30219862-30219881 MS.gene96101:CDS 40.0%
CAACTTATCATATGGCACCA+AGG + chr7.4:30219117-30219136 MS.gene96101:CDS 40.0%
CATATCATTACCGTCAATGC+AGG + chr7.4:30219673-30219692 MS.gene96101:CDS 40.0%
CATGTAACTGTTCCTGAATC+TGG + chr7.4:30219158-30219177 MS.gene96101:CDS 40.0%
GTTTCTTTGTCAAGTCCTGA+TGG + chr7.4:30220320-30220339 MS.gene96101:CDS 40.0%
TTTGACCTATGAGGTGATTC+TGG + chr7.4:30219933-30219952 MS.gene96101:intron 40.0%
! AGTCCTATTTTGTGTGACAG+AGG - chr7.4:30220027-30220046 None:intergenic 40.0%
! GCATTGACGGTAATGATATG+CGG - chr7.4:30219673-30219692 None:intergenic 40.0%
! GGTGCCATATGATAAGTTGA+TGG - chr7.4:30219116-30219135 None:intergenic 40.0%
! TGTTACTTCTGAAGGTGACT+CGG + chr7.4:30219630-30219649 MS.gene96101:intron 40.0%
! TGTTCCTGAATCTGGTAATG+TGG + chr7.4:30219166-30219185 MS.gene96101:intron 40.0%
!! AGTTTTCAGGGTTTGAACAC+CGG + chr7.4:30219497-30219516 MS.gene96101:intron 40.0%
!! GGGACACTAAAAGTGTTTCA+AGG - chr7.4:30219527-30219546 None:intergenic 40.0%
AAACACTCATTCCACCAGAC+CGG - chr7.4:30220304-30220323 None:intergenic 45.0%
AAAGGAGCTGAAGACGAGAT+TGG - chr7.4:30219311-30219330 None:intergenic 45.0%
AAAGTTCCGCCAGAAGAATC+AGG - chr7.4:30219916-30219935 None:intergenic 45.0%
AAATCACGCCACTAGCAGAT+AGG - chr7.4:30219873-30219892 None:intergenic 45.0%
ACAACCAAGGAACAAGAAGC+CGG + chr7.4:30220282-30220301 MS.gene96101:CDS 45.0%
AGAAGAAAAGGGGTAGACCA+AGG + chr7.4:30219237-30219256 MS.gene96101:intron 45.0%
AGAGCCACATTACCAGATTC+AGG - chr7.4:30219173-30219192 None:intergenic 45.0%
AGGGGTAGACCAAGGAAATA+TGG + chr7.4:30219245-30219264 MS.gene96101:intron 45.0%
GATAGGATGCATATAGCTCG+CGG - chr7.4:30219856-30219875 None:intergenic 45.0%
GCTTCTTGTTCCTTGGTTGT+CGG - chr7.4:30220282-30220301 None:intergenic 45.0%
GGAACAGTTACATGTTGGGT+TGG - chr7.4:30219152-30219171 None:intergenic 45.0%
GGGGTAGACCAAGGAAATAT+GGG + chr7.4:30219246-30219265 MS.gene96101:intron 45.0%
GTATCTCAAATCGACCCTAG+GGG - chr7.4:30220232-30220251 None:intergenic 45.0%
! AATTTTCATCCGGGAAGCAG+GGG + chr7.4:30219339-30219358 MS.gene96101:intron 45.0%
! GAATCTGGTAATGTGGCTCT+TGG + chr7.4:30219173-30219192 MS.gene96101:intron 45.0%
! GAATTTTCATCCGGGAAGCA+GGG + chr7.4:30219338-30219357 MS.gene96101:intron 45.0%
! TGAATTTTCATCCGGGAAGC+AGG + chr7.4:30219337-30219356 MS.gene96101:intron 45.0%
!! AAAAGTGTTTCAAGGAGGGC+CGG - chr7.4:30219519-30219538 None:intergenic 45.0%
AAAGCTGGAGCTGATCCTCT+AGG + chr7.4:30219395-30219414 MS.gene96101:intron 50.0%
ACCATCCCTCGCAAATAGCT+CGG - chr7.4:30219562-30219581 None:intergenic 50.0%
AGTGGCAGCTACAAGAAGAC+CGG - chr7.4:30220369-30220388 None:intergenic 50.0%
ATAGCTCGGTTGGCAAGCTA+AGG - chr7.4:30219548-30219567 None:intergenic 50.0%
CAAGGAAATATGGGCCAGAC+GGG + chr7.4:30219255-30219274 MS.gene96101:intron 50.0%
CCAAGGAAATATGGGCCAGA+CGG + chr7.4:30219254-30219273 MS.gene96101:intron 50.0%
CTTCGTCAGCCTGATTCTTC+TGG + chr7.4:30219904-30219923 MS.gene96101:CDS 50.0%
CTTGTTCCTTGGTTGTCGGT+AGG - chr7.4:30220278-30220297 None:intergenic 50.0%
GCTGAAGACGAGATTGGCAA+TGG - chr7.4:30219305-30219324 None:intergenic 50.0%
GGGAGAGAAGGTAACAACAC+TGG + chr7.4:30219065-30219084 MS.gene96101:CDS 50.0%
TAGCTCGGTTGGCAAGCTAA+GGG - chr7.4:30219547-30219566 None:intergenic 50.0%
TGCTTGAACTCCATCCCCTT+CGG - chr7.4:30219368-30219387 None:intergenic 50.0%
TGGCGGAACTTTGACCTATG+AGG + chr7.4:30219924-30219943 MS.gene96101:CDS 50.0%
TTCATGCCTACCGACAACCA+AGG + chr7.4:30220269-30220288 MS.gene96101:CDS 50.0%
!! ACTTCTGAAGGTGACTCGGT+CGG + chr7.4:30219634-30219653 MS.gene96101:intron 50.0%
ACCGAGCTATTTGCGAGGGA+TGG + chr7.4:30219558-30219577 MS.gene96101:intron 55.0%
AGTCCTGATGGTCGTGTCGT+AGG + chr7.4:30220332-30220351 MS.gene96101:CDS 55.0%
ATCACCGGTGAGTCTGGGAA+TGG + chr7.4:30219199-30219218 MS.gene96101:intron 55.0%
CAAGGAACAAGAAGCCGGTC+TGG + chr7.4:30220287-30220306 MS.gene96101:CDS 55.0%
CATTACCGTCAATGCAGGCG+AGG + chr7.4:30219678-30219697 MS.gene96101:CDS 55.0%
CCATCCATTCCCAGACTCAC+CGG - chr7.4:30219206-30219225 None:intergenic 55.0%
CCGTCTGGCCCATATTTCCT+TGG - chr7.4:30219257-30219276 None:intergenic 55.0%
CGTCAGCCTGATTCTTCTGG+CGG + chr7.4:30219907-30219926 MS.gene96101:CDS 55.0%
GAAATATGGGCCAGACGGGT+CGG + chr7.4:30219259-30219278 MS.gene96101:intron 55.0%
GCATACCTCGCCTGCATTGA+CGG - chr7.4:30219686-30219705 None:intergenic 55.0%
GCCAACCGAGCTATTTGCGA+GGG + chr7.4:30219554-30219573 MS.gene96101:intron 55.0%
GTGAGTCTGGGAATGGATGG+AGG + chr7.4:30219206-30219225 MS.gene96101:intron 55.0%
TCCCTCGCAAATAGCTCGGT+TGG - chr7.4:30219558-30219577 None:intergenic 55.0%
TCGCGGTCCTTGTTGCGAAA+AGG - chr7.4:30219839-30219858 None:intergenic 55.0%
TGCCAACCGAGCTATTTGCG+AGG + chr7.4:30219553-30219572 MS.gene96101:intron 55.0%
TGTGGCTCTTGGCGTATCAC+CGG + chr7.4:30219184-30219203 MS.gene96101:intron 55.0%
! CAGACCGGCTTCTTGTTCCT+TGG - chr7.4:30220289-30220308 None:intergenic 55.0%
! TGGGTTGGAGCTTCACTCCT+TGG - chr7.4:30219137-30219156 None:intergenic 55.0%
ACAGCAGTAACCGACCCGTC+TGG - chr7.4:30219272-30219291 None:intergenic 60.0%
AGACGGGTCGGTTACTGCTG+TGG + chr7.4:30219271-30219290 MS.gene96101:intron 60.0%
CCGGTGAGTCTGGGAATGGA+TGG + chr7.4:30219203-30219222 MS.gene96101:intron 60.0%
CCTGATGGTCGTGTCGTAGG+CGG + chr7.4:30220335-30220354 MS.gene96101:CDS 60.0%
CTTCGGTTTCCCCTGCTTCC+CGG - chr7.4:30219351-30219370 None:intergenic 60.0%
GGAACAAGAAGCCGGTCTGG+TGG + chr7.4:30220290-30220309 MS.gene96101:CDS 60.0%
GGAAGCAGGGGAAACCGAAG+GGG + chr7.4:30219351-30219370 MS.gene96101:intron 60.0%
GGCGTATCACCGGTGAGTCT+GGG + chr7.4:30219194-30219213 MS.gene96101:intron 60.0%
GGGAAGCAGGGGAAACCGAA+GGG + chr7.4:30219350-30219369 MS.gene96101:intron 60.0%
TGGCGTATCACCGGTGAGTC+TGG + chr7.4:30219193-30219212 MS.gene96101:intron 60.0%
CCGCCTACGACACGACCATC+AGG - chr7.4:30220338-30220357 None:intergenic 65.0%
CGGGAAGCAGGGGAAACCGA+AGG + chr7.4:30219349-30219368 MS.gene96101:intron 65.0%
GATGGTCGTGTCGTAGGCGG+TGG + chr7.4:30220338-30220357 MS.gene96101:CDS 65.0%
GCAGGGGAAACCGAAGGGGA+TGG + chr7.4:30219355-30219374 MS.gene96101:intron 65.0%
GTCGTAGGCGGTGGAGTTGC+CGG + chr7.4:30220347-30220366 MS.gene96101:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 30219059 30220397 30219059 ID=MS.gene96101
chr7.4 mRNA 30219059 30220397 30219059 ID=MS.gene96101.t1;Parent=MS.gene96101
chr7.4 exon 30219059 30219179 30219059 ID=MS.gene96101.t1.exon1;Parent=MS.gene96101.t1
chr7.4 CDS 30219059 30219179 30219059 ID=cds.MS.gene96101.t1;Parent=MS.gene96101.t1
chr7.4 exon 30219644 30219699 30219644 ID=MS.gene96101.t1.exon2;Parent=MS.gene96101.t1
chr7.4 CDS 30219644 30219699 30219644 ID=cds.MS.gene96101.t1;Parent=MS.gene96101.t1
chr7.4 exon 30219814 30219945 30219814 ID=MS.gene96101.t1.exon3;Parent=MS.gene96101.t1
chr7.4 CDS 30219814 30219945 30219814 ID=cds.MS.gene96101.t1;Parent=MS.gene96101.t1
chr7.4 exon 30220236 30220397 30220236 ID=MS.gene96101.t1.exon4;Parent=MS.gene96101.t1
chr7.4 CDS 30220236 30220397 30220236 ID=cds.MS.gene96101.t1;Parent=MS.gene96101.t1
Gene Sequence

>MS.gene96101

ATGGAGGGGAGAGAAGGTAACAACACTGGAGTAACAGTGATAGGAGCAGAAGCTCCATCAACTTATCATATGGCACCAAGGAGTGAAGCTCCAACCCAACATGTAACTGTTCCTGAATCTGGTAATGTGGCTCTTGGCGTATCACCGGTGAGTCTGGGAATGGATGGAGGAACAATTAAGAAGAAAAGGGGTAGACCAAGGAAATATGGGCCAGACGGGTCGGTTACTGCTGTGGCGTTGTCACCATTGCCAATCTCGTCTTCAGCTCCTTTTTCTAATGAATTTTCATCCGGGAAGCAGGGGAAACCGAAGGGGATGGAGTTCAAGCAGTCAAAAAAAGCTGGAGCTGATCCTCTAGGTATTTTTGATTTGATAGTTTTAGTGTTACTAATCATTCCTTTTTTTATAGACCAATATTAGTAAGTTAGTAGTTAAGTTAGTTTTCAGGGTTTGAACACCGGCCCTCCTTGAAACACTTTTAGTGTCCCTTAGCTTGCCAACCGAGCTATTTGCGAGGGATGGTACTGATGATTCCTAGTATACAACAAAATTATGTGCCACTAACTATTGTTGTTACTTCTGAAGGTGACTCGGTCGGTACCAACTTTATGCCGCATATCATTACCGTCAATGCAGGCGAGGTATGCTTGAGAGATAACATATCTTTAAATTAGAAATATGCATCTAATGTTAATTCTTCTTTGGGAAGAAACAAGTATGTCATGTTAATTTACTTTACTCTTTTGTTGTTTCAGGACATTACAATGAAGGTTATATCCTTTTCGCAACAAGGACCGCGAGCTATATGCATCCTATCTGCTAGTGGCGTGATTTCAAATGTTACGCTTCGTCAGCCTGATTCTTCTGGCGGAACTTTGACCTATGAGGTGATTCTGGTTCTTTTAAGTTTCAAACTTCAGTTTTTTATATGTACATCTTTAATATCGGTTGAAATTAATATCAAACCTCTGTCACACAAAATAGGACTTCGTTCGGTTTTAGTTTCTCTAATTACAAACTTCAAGCAACATTCATGTGGAAATTACAGTAACTTTGTAGTATAACCTCGTATAAGTTCAACATTCATGTGGAAACCATAGTTTTTCATTTTCATTGATTATTGGTATGAATTTTGTTTTGATTGGTACAAAATATCATGTTTCATTTTATCCCCTAGGGTCGATTTGAGATACTTTCACTGTCTGGATCGTTCATGCCTACCGACAACCAAGGAACAAGAAGCCGGTCTGGTGGAATGAGTGTTTCTTTGTCAAGTCCTGATGGTCGTGTCGTAGGCGGTGGAGTTGCCGGTCTTCTTGTAGCTGCCACTCCTGTGCAG
Protein sequence

>MS.gene96101.t1

MEGREGNNTGVTVIGAEAPSTYHMAPRSEAPTQHVTVPESGDSVGTNFMPHIITVNAGEDITMKVISFSQQGPRAICILSASGVISNVTLRQPDSSGGTLTYEGRFEILSLSGSFMPTDNQGTRSRSGGMSVSLSSPDGRVVGGGVAGLLVAATPVQ