AlfalfaGEDB | MS.gene058128

M. sativa cultivar XinJiangDaYe / MS.gene058128

KEGG: K03363
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00400.35 PF12894.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058128.t1	XP_003610876.1	99.1	454	4	0	1	454	1	454	1.50E-265	924.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058128.t1	Q9SZA4	79.8	450	89	2	7	454	8	457	2.4e-216	752.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058128.t1	G7K6J6	99.1	454	4	0	1	454	1	454	1.1e-265	924.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene058128	0.863479	2.42E-64	-1.69E-46
MS.gene050707	MS.gene058128	0.831606	1.52E-55	-1.69E-46
MS.gene050875	MS.gene058128	0.804717	1.89E-49	-1.69E-46
MS.gene051896	MS.gene058128	0.818671	1.74E-52	-1.69E-46
MS.gene051914	MS.gene058128	0.867474	1.33E-65	-1.69E-46
MS.gene051955	MS.gene058128	0.805517	1.28E-49	-1.69E-46
MS.gene052308	MS.gene058128	0.899383	2.09E-77	-1.69E-46
MS.gene052367	MS.gene058128	0.803516	3.36E-49	-1.69E-46
MS.gene053173	MS.gene058128	0.846608	1.95E-59	-1.69E-46
MS.gene053348	MS.gene058128	0.858815	6.36E-63	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene010910	MS.gene058128	PPI
MS.gene010912	MS.gene058128	PPI
MS.gene011448	MS.gene058128	PPI
MS.gene012203	MS.gene058128	PPI
MS.gene027071	MS.gene058128	PPI
MS.gene027074	MS.gene058128	PPI
MS.gene030294	MS.gene058128	PPI
MS.gene037950	MS.gene058128	PPI
MS.gene039682	MS.gene058128	PPI
MS.gene049022	MS.gene058128	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058128.t1	MTR_5g008010	99.119	454	4	0	1	454	1	454	0.0	937
MS.gene058128.t1	MTR_3g051690	91.503	459	34	4	1	454	1	459	0.0	872
MS.gene058128.t1	MTR_7g106870	75.223	448	105	5	12	454	11	457	0.0	707
MS.gene058128.t1	MTR_7g451830	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene058128.t1	MTR_7g047640	66.966	445	126	7	15	454	3	431	0.0	591
MS.gene058128.t1	MTR_7g057540	65.011	443	135	7	14	454	2	426	0.0	563
MS.gene058128.t1	MTR_7g055590	64.932	442	135	7	14	453	2	425	0.0	561
MS.gene058128.t1	MTR_7g053100	61.607	448	147	8	17	454	4	436	0.0	537
MS.gene058128.t1	MTR_7g034625	71.160	319	90	1	124	440	13	331	1.14e-172	488
MS.gene058128.t1	MTR_2g100540	51.945	437	160	13	25	454	4	397	2.69e-135	395

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058128.t1	AT4G33270	79.778	450	89	2	7	454	8	457	0.0	759
MS.gene058128.t1	AT4G33260	80.317	442	85	2	15	454	6	447	0.0	737
MS.gene058128.t1	AT4G33260	80.535	411	78	2	15	423	6	416	0.0	701
MS.gene058128.t1	AT5G27570	69.623	451	130	5	7	454	4	450	0.0	642
MS.gene058128.t1	AT5G27080	69.585	434	126	4	23	454	13	442	0.0	623
MS.gene058128.t1	AT5G26900	68.578	436	132	3	20	454	13	444	0.0	612
MS.gene058128.t1	AT5G27945	62.634	372	123	5	95	454	61	428	1.40e-162	466
MS.gene058128.t1	AT4G22910	42.822	411	217	4	33	433	62	464	3.27e-115	347
MS.gene058128.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335
MS.gene058128.t1	AT5G13840	51.974	304	138	3	126	429	163	458	1.13e-110	335

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Find 128 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCATCGCCGATCTATT+CGG	0.388532	5.4:-3187966	None:intergenic
AACAACCACATACTCACAAC+CGG	0.499451	5.4:+3188984	MS.gene058128:CDS
AACCACATACTCACAACCGG+AGG	0.695066	5.4:+3188987	MS.gene058128:CDS
AACCGAGTTCAAGCATGCAC+CGG	0.591621	5.4:-3189327	None:intergenic
AACGGTCAACAATTAGCCAG+TGG	0.669539	5.4:+3189113	MS.gene058128:CDS
AAGAAGTTTGCGGATTGAAA+TGG	0.432752	5.4:+3189084	MS.gene058128:CDS
AAGATAAAACCATAAAGCCC+AGG	0.612129	5.4:+3188189	MS.gene058128:CDS
AAGTGCTATTGCAAGAACAT+TGG	0.617527	5.4:-3188643	None:intergenic
AATAATTCGCCTGGGCTTTA+TGG	0.172694	5.4:-3188198	None:intergenic
AATGGCTCAGCTCTTGCTTT+CGG	0.359726	5.4:-3189611	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATTGTTGAAACATACAGA+GGG	+	chr5.4:3189054-3189073	MS.gene058128:CDS	30.0%
CACTTCAAGAGTTCTTTATA+TGG	+	chr5.4:3189487-3189506	MS.gene058128:CDS	30.0%
CAGATTTATACCGAATAGAT+CGG	+	chr5.4:3187956-3187975	MS.gene058128:CDS	30.0%
CTTAGTTAAAAGGGTTTGTA+TGG	+	chr5.4:3188360-3188379	MS.gene058128:intron	30.0%
TACCTCAATTTATTAGACTG+GGG	+	chr5.4:3188615-3188634	MS.gene058128:CDS	30.0%
TTGCCAACATTATTCTTGTT+TGG	+	chr5.4:3188901-3188920	MS.gene058128:intron	30.0%
AAGATAAAACCATAAAGCCC+AGG	+	chr5.4:3188189-3188208	MS.gene058128:CDS	35.0%
AAGGTGTATTAAGGTTAACC+AGG	-	chr5.4:3188856-3188875	None:intergenic	35.0%
AATAATGTTGGCAAACTAGC+AGG	-	chr5.4:3188895-3188914	None:intergenic	35.0%
AATGTTCTTGCAATAGCACT+TGG	+	chr5.4:3188645-3188664	MS.gene058128:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	3187418	3189658	3187418	ID=MS.gene058128
chr5.4	mRNA	3187418	3189658	3187418	ID=MS.gene058128.t1;Parent=MS.gene058128
chr5.4	exon	3187418	3187510	3187418	ID=MS.gene058128.t1.exon1;Parent=MS.gene058128.t1
chr5.4	CDS	3187418	3187510	3187418	ID=cds.MS.gene058128.t1;Parent=MS.gene058128.t1
chr5.4	exon	3187951	3188226	3187951	ID=MS.gene058128.t1.exon2;Parent=MS.gene058128.t1
chr5.4	CDS	3187951	3188226	3187951	ID=cds.MS.gene058128.t1;Parent=MS.gene058128.t1
chr5.4	exon	3188570	3188839	3188570	ID=MS.gene058128.t1.exon3;Parent=MS.gene058128.t1
chr5.4	CDS	3188570	3188839	3188570	ID=cds.MS.gene058128.t1;Parent=MS.gene058128.t1
chr5.4	exon	3188933	3189658	3188933	ID=MS.gene058128.t1.exon4;Parent=MS.gene058128.t1
chr5.4	CDS	3188933	3189658	3188933	ID=cds.MS.gene058128.t1;Parent=MS.gene058128.t1

Gene Sequence

>MS.gene058128

ATGGCTTCAGGTTCTTTGAGTTCTCCCGCCATCAAAACCCAATCACGTTTTCCTCTTCAGGAACACTTACAGAGAAAGAGTTCCAAGGAAAATGTAAGAACATTTTTCAATTATTCAGATCTTTCAAAATTAGGGTTTTAATGTAAATTGGGATTTTAATTATCAAATTTTGGTGTTTTGGCCATAATTGGATTAGTAGTCTAAAAACTGTGATAAAGAATAAATTTTGAAATTTTGGGGGTTTTCTTAGATTGTGAATAAATTTTAAAATTGGGATTTTTAATTTTCGAATTGGGGGTTTTCTTAGATTGTGGATTAATTTTGAAAATATGTTAAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGACATTATGTCATGAAAAAGAATGAATTTTGAAATTATGCCAAACTTTTTATTGACTGAGTTGAAATTATGATATGATAAGTTGATAATATTCTGAATTTGTTTATGAAAATCAAGTATTGATTCTTTATTGTTTGATTTGTTTGTTGCCAGTTGGACAGATTTATACCGAATAGATCGGCGATGGATTTCGATTATGCACACTACATGGTGACAGAAGGGGCTAAAGGTAAGGAAAATCCAGAAGTGTGCTCCCCTTCCAGAGAAGCGTACAGGAAGCTACTTGCAGAGTCTTTAAACATGAACAGGACCAGAATTCTAGCTTTCAAGAACAAGCCACCTACTCCAGTTGATTTGATTCCTCATGAGCTTACTTCATCATCTCTTCAACAAGATAAAACCATAAAGCCCAGGCGAATTATTCCTCAGGTGTCTTACTGAAATCTGATTGATATTCCTTATGTTTTATCATTGTTATTTTGGTTCTTTGGTTGCATGAACTCCAAATTTAAATTTTGAATGAGTTTTGAAAATCAAGTGGGTCTGAGTTGTTTTATTAATTCTTAGTTAAAAGGGTTTGTATGGTGAATGAAATTCAATATTTTGCAATTTTGTGCTAGTTGATGATTAGTTGGCTATCTGATTAGGTTTTTGAAATTCAAGTGGATCTGAGTTGTTTTCTTAATTCTTAGTTGAAAGTGTTGTATGGTATAAACTGTAAACCATAATATTTTGCAATTTTGTGCTAATTGATTGATATGTTGTTTTTCAGACTTCTGAGAGGACTTTGGACGCTCCAGACCTTGTGGATGACTATTACCTCAATTTATTAGACTGGGGAAGCGCCAATGTTCTTGCAATAGCACTTGGAAACACAGTGTATTTGTGGGATGCATCAAATGGTTCTACTTCAGAACTTGTTACCGTCGAAGAAGAAGACGGCCCTATCACATCTGTGAGTTGGGCTCCTGATGGGCGTCATATTGGCATTGGTTTGAACAACTCTGAAGTGCAGCTATGGGATACAGCTTCAGATAAACAGGTTTTTACACTTACCTGGTTAACCTTAATACACCTTTATGAACTATCAAATTCCTGCTAGTTTGCCAACATTATTCTTGTTTGGTTTCTGCAGTTGAGAACTTTGAAAGGTGGTCATAGGCAGCGAGTAGGGTCTTTGGCATGGAACAACCACATACTCACAACCGGAGGGATGGACGGTAAAATTATCAATAATGATGTAAGAATTAGAGCACACATTGTTGAAACATACAGAGGGCATGAACAAGAAGTTTGCGGATTGAAATGGTCGGCTAACGGTCAACAATTAGCCAGTGGAGGGAATGATAATCTACTTTACATCTGGGACAGAGGTACATCAACTTCTAGTTCTCCTACACAGTGGCTTCACAGGCTTGAAGATCATACATCTGCAGTAAAGGCTCTTGCTTGGTGTCCATTCCAAGGGAATTTGCTAGCTACTGGTGGAGGCAGTGGCGATCGAACAATCAAGTTTTGGAATACCCACACCGGTGCATGCTTGAACTCGGTTGACACTGGGTCCCAAGTATGTTCCCTGCTATGGAACAAGAATGAAAGGGAGTTACTCAGCTCTCATGGTTTTACACAGAATCAGCTAACACTTTGGAAATATCCTTCAATGGTGAAGATGGCAGAGCTCAATGGTCACACTTCAAGAGTTCTTTATATGGCGCAGAGCCCGGATGGTTGCACAGTGGCAACAGCAGCAGCAGACGAAACATTGAGGTTTTGGAATGCTTTTGGCACTCCAGAAGTAGTAGCAAAAGCTGCACCGAAAGCAAGAGCTGAGCCATTTTCACATGTGAGCCGCATTCGCTAA

Protein sequence

>MS.gene058128.t1

MASGSLSSPAIKTQSRFPLQEHLQRKSSKENLDRFIPNRSAMDFDYAHYMVTEGAKGKENPEVCSPSREAYRKLLAESLNMNRTRILAFKNKPPTPVDLIPHELTSSSLQQDKTIKPRRIIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWDASNGSTSELVTVEEEDGPITSVSWAPDGRHIGIGLNNSEVQLWDTASDKQLRTLKGGHRQRVGSLAWNNHILTTGGMDGKIINNDVRIRAHIVETYRGHEQEVCGLKWSANGQQLASGGNDNLLYIWDRGTSTSSSPTQWLHRLEDHTSAVKALAWCPFQGNLLATGGGSGDRTIKFWNTHTGACLNSVDTGSQVCSLLWNKNERELLSSHGFTQNQLTLWKYPSMVKMAELNGHTSRVLYMAQSPDGCTVATAAADETLRFWNAFGTPEVVAKAAPKARAEPFSHVSRIR