Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97300.t1 | XP_003621633.1 | 99.1 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 7.30E-57 | 229.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97300.t1 | O49884 | 99.1 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 3.0e-59 | 228.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97300.t1 | G7KSW7 | 99.1 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 5.3e-57 | 229.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050252 | MS.gene97300 | 0.824008 | 1.02E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene97300 | MS.gene23883 | PPI |
| MS.gene97300 | MS.gene074262 | PPI |
| MS.gene97300 | MS.gene43173 | PPI |
| MS.gene97300 | MS.gene022106 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97300.t1 | MTR_7g021030 | 99.107 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 2.99e-79 | 228 |
| MS.gene97300.t1 | MTR_0024s0220 | 97.321 | 112 | 3 | 0 | 1 | 112 | 1 | 112 | 6.06e-78 | 225 |
| MS.gene97300.t1 | MTR_5g088740 | 91.964 | 112 | 9 | 0 | 1 | 112 | 1 | 112 | 2.18e-74 | 216 |
| MS.gene97300.t1 | MTR_0024s0220 | 97.030 | 101 | 3 | 0 | 1 | 101 | 1 | 101 | 6.57e-69 | 202 |
| MS.gene97300.t1 | MTR_7g021030 | 96.203 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 3.46e-50 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97300.t1 | AT1G36240 | 88.393 | 112 | 13 | 0 | 1 | 112 | 1 | 112 | 1.23e-71 | 209 |
| MS.gene97300.t1 | AT3G18740 | 87.500 | 112 | 14 | 0 | 1 | 112 | 1 | 112 | 9.70e-71 | 207 |
| MS.gene97300.t1 | AT1G77940 | 86.607 | 112 | 15 | 0 | 1 | 112 | 1 | 112 | 9.70e-71 | 207 |
Find 26 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAACAACTGTCCTCCTTTG+AGG | 0.365472 | 7.3:-78633609 | MS.gene97300:CDS |
| GACATCATTAAGACAGTTCC+TGG | 0.370725 | 7.3:-78632489 | MS.gene97300:CDS |
| ACAACTTACTTCCATTGTAA+TGG | 0.429111 | 7.3:+78633539 | None:intergenic |
| GAAGCTTACAGTCTTCTTTG+CGG | 0.454853 | 7.3:+78634134 | None:intergenic |
| CTCATGAGAGCATCAACAAC+AGG | 0.462326 | 7.3:-78634013 | MS.gene97300:CDS |
| TGCCTCAGCATTGTTGATCC+TGG | 0.466033 | 7.3:-78632603 | MS.gene97300:intron |
| TACTACGTTTACTGATCTCC+AGG | 0.471605 | 7.3:+78632471 | None:intergenic |
| AATTACCTTTAGAACTCCTA+AGG | 0.474325 | 7.3:+78633931 | None:intergenic |
| AAGAGTGGAAAATATACCCT+TGG | 0.505811 | 7.3:-78633975 | MS.gene97300:CDS |
| AAACCGTTCTCAAATCCCTT+AGG | 0.509696 | 7.3:-78633947 | MS.gene97300:CDS |
| ATTACCTTTAGAACTCCTAA+GGG | 0.521637 | 7.3:+78633932 | None:intergenic |
| TATGCTATGTTGGCTAAGGT+TGG | 0.529938 | 7.3:-78633568 | MS.gene97300:CDS |
| AAATCCCTTAGGAGTTCTAA+AGG | 0.532626 | 7.3:-78633936 | MS.gene97300:intron |
| CTCGCTCTTGTCATGAAGAG+TGG | 0.545906 | 7.3:-78633990 | MS.gene97300:CDS |
| AATTGAGTACTATGCTATGT+TGG | 0.552970 | 7.3:-78633578 | MS.gene97300:CDS |
| AGAGTGGAAAATATACCCTT+GGG | 0.553555 | 7.3:-78633974 | MS.gene97300:CDS |
| GTACTATGCTATGTTGGCTA+AGG | 0.556485 | 7.3:-78633572 | MS.gene97300:CDS |
| GTTGACTTGGGCACTGCTTG+CGG | 0.576654 | 7.3:-78632645 | MS.gene97300:CDS |
| ACTCCTAAGGGATTTGAGAA+CGG | 0.577693 | 7.3:+78633944 | None:intergenic |
| ATTTCAGACTTCCTCAAAGG+AGG | 0.587118 | 7.3:+78633598 | None:intergenic |
| AGGAACTGTCTTAATGATGT+CGG | 0.588072 | 7.3:+78632491 | None:intergenic |
| GTTATCAGACAATGTTGACT+TGG | 0.596186 | 7.3:-78632658 | MS.gene97300:intron |
| TCAATTTCAGACTTCCTCAA+AGG | 0.597003 | 7.3:+78633595 | None:intergenic |
| GTTGGAGTTCACCATTACAA+TGG | 0.600433 | 7.3:-78633550 | MS.gene97300:CDS |
| TACCAGGATCAACAATGCTG+AGG | 0.659030 | 7.3:+78632601 | None:intergenic |
| TTATCAGACAATGTTGACTT+GGG | 0.668297 | 7.3:-78632657 | MS.gene97300:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGACAAATAAAATTAGA+GGG | + | chr7.3:78634092-78634111 | None:intergenic | 15.0% |
| !!! | TTTGATTTGATTATTTTGTA+GGG | - | chr7.3:78632972-78632991 | MS.gene97300:intron | 15.0% |
| !!! | TTTTGATTTGATTATTTTGT+AGG | - | chr7.3:78632971-78632990 | MS.gene97300:intron | 15.0% |
| !! | CAAATGACAAATAAAATTAG+AGG | + | chr7.3:78634093-78634112 | None:intergenic | 20.0% |
| !!! | CTCTAATTTTATTTGTCATT+TGG | - | chr7.3:78634091-78634110 | MS.gene97300:intron | 20.0% |
| !!! | TTGATTGAATTAAGTGATTT+TGG | - | chr7.3:78632912-78632931 | MS.gene97300:intron | 20.0% |
| !!! | TTTTATCAATTGTCTCTTTA+AGG | - | chr7.3:78633889-78633908 | MS.gene97300:intron | 20.0% |
| ! | AAATAAGTGATTAGTGAAAG+TGG | - | chr7.3:78632866-78632885 | MS.gene97300:intron | 25.0% |
| ! | ACATTGAAAAATGAACAACA+CGG | + | chr7.3:78633133-78633152 | None:intergenic | 25.0% |
| ! | AGTTGACAATTAACATCAAT+AGG | + | chr7.3:78633857-78633876 | None:intergenic | 25.0% |
| ! | ATGATTTCGTTTCAGATTTA+GGG | - | chr7.3:78632538-78632557 | MS.gene97300:intron | 25.0% |
| ! | TACACATTTCAACAACAAAA+TGG | + | chr7.3:78632723-78632742 | None:intergenic | 25.0% |
| ! | TATGATTTCGTTTCAGATTT+AGG | - | chr7.3:78632537-78632556 | MS.gene97300:intron | 25.0% |
| ! | TTAAATACTGACACAGTTAA+AGG | + | chr7.3:78634055-78634074 | None:intergenic | 25.0% |
| ! | TTAGATTAGATTAGATTGCA+AGG | - | chr7.3:78632781-78632800 | MS.gene97300:intron | 25.0% |
| !! | TACATTAACAGAAGCATATT+TGG | + | chr7.3:78633689-78633708 | None:intergenic | 25.0% |
| !! | TCAGTTAGTTTAATCAATCA+GGG | + | chr7.3:78633226-78633245 | None:intergenic | 25.0% |
| !! | TTCAGTTAGTTTAATCAATC+AGG | + | chr7.3:78633227-78633246 | None:intergenic | 25.0% |
| !! | TTGTTGTTGAAATGTGTATT+GGG | - | chr7.3:78632725-78632744 | MS.gene97300:intron | 25.0% |
| !!! | TGACTTAACTTTTAAGATTC+TGG | - | chr7.3:78633524-78633543 | MS.gene97300:intron | 25.0% |
| !!! | TTTGTTGTTGAAATGTGTAT+TGG | - | chr7.3:78632724-78632743 | MS.gene97300:intron | 25.0% |
| AACTATGCATATGTAACAAG+TGG | + | chr7.3:78633717-78633736 | None:intergenic | 30.0% | |
| AATTATTACATGCGACCATA+AGG | + | chr7.3:78633415-78633434 | None:intergenic | 30.0% | |
| AATTGAGTACTATGCTATGT+TGG | - | chr7.3:78633042-78633061 | MS.gene97300:intron | 30.0% | |
| ACAACTTACTTCCATTGTAA+TGG | + | chr7.3:78633084-78633103 | None:intergenic | 30.0% | |
| ATTATTACATGCGACCATAA+GGG | + | chr7.3:78633414-78633433 | None:intergenic | 30.0% | |
| GTGTTTGGATAGATGTTATA+AGG | + | chr7.3:78633359-78633378 | None:intergenic | 30.0% | |
| TAAAGGAAGCAATATATACC+AGG | + | chr7.3:78634038-78634057 | None:intergenic | 30.0% | |
| TAGGAAGAATTCCAACAATT+TGG | + | chr7.3:78633838-78633857 | None:intergenic | 30.0% | |
| TATTATGCATATGCTATTGC+TGG | - | chr7.3:78633765-78633784 | MS.gene97300:intron | 30.0% | |
| TCTATCCAAACACTACATTA+CGG | - | chr7.3:78633366-78633385 | MS.gene97300:intron | 30.0% | |
| TTTAGTGAATGCTTGTATCT+GGG | - | chr7.3:78633248-78633267 | MS.gene97300:intron | 30.0% | |
| TTTGGATAGATGTTATAAGG+TGG | + | chr7.3:78633356-78633375 | None:intergenic | 30.0% | |
| !! | AATTACCTTTAGAACTCCTA+AGG | + | chr7.3:78632692-78632711 | None:intergenic | 30.0% |
| !! | ATTACCTTTAGAACTCCTAA+GGG | + | chr7.3:78632691-78632710 | None:intergenic | 30.0% |
| !! | GTTGTAGATTGATTTTGTAG+TGG | - | chr7.3:78632843-78632862 | MS.gene97300:intron | 30.0% |
| !! | TTATCAGACAATGTTGACTT+GGG | - | chr7.3:78633963-78633982 | MS.gene97300:CDS | 30.0% |
| !! | TTGTTGTTTCTCCAAATTGT+TGG | - | chr7.3:78633824-78633843 | MS.gene97300:intron | 30.0% |
| !! | TTTGAATGTTGATTTGGTAG+TGG | - | chr7.3:78632938-78632957 | MS.gene97300:intron | 30.0% |
| !!! | ATTTGTCATTTGGTGTTTTC+AGG | - | chr7.3:78634101-78634120 | MS.gene97300:intron | 30.0% |
| !!! | TGGAGTTTTGAATGTTGATT+TGG | - | chr7.3:78632932-78632951 | MS.gene97300:intron | 30.0% |
| AAGAGTGGAAAATATACCCT+TGG | - | chr7.3:78632645-78632664 | MS.gene97300:CDS | 35.0% | |
| AATCTTTCAGCACTTAGATG+TGG | - | chr7.3:78633634-78633653 | MS.gene97300:intron | 35.0% | |
| AGAGTGGAAAATATACCCTT+GGG | - | chr7.3:78632646-78632665 | MS.gene97300:CDS | 35.0% | |
| AGGAACTGTCTTAATGATGT+CGG | + | chr7.3:78634132-78634151 | None:intergenic | 35.0% | |
| ATTGACCGTAATGTAGTGTT+TGG | + | chr7.3:78633374-78633393 | None:intergenic | 35.0% | |
| ATTTGATCTTATGCTGCTGA+TGG | - | chr7.3:78633562-78633581 | MS.gene97300:CDS | 35.0% | |
| CTTTAGTGAATGCTTGTATC+TGG | - | chr7.3:78633247-78633266 | MS.gene97300:intron | 35.0% | |
| GTTGTGTATGTATACCCTTA+TGG | - | chr7.3:78633397-78633416 | MS.gene97300:intron | 35.0% | |
| TCAATTTCAGACTTCCTCAA+AGG | + | chr7.3:78633028-78633047 | None:intergenic | 35.0% | |
| TGTTATAAGGTGGAATAGAG+TGG | + | chr7.3:78633346-78633365 | None:intergenic | 35.0% | |
| TTTGATCTTATGCTGCTGAT+GGG | - | chr7.3:78633563-78633582 | MS.gene97300:CDS | 35.0% | |
| ! | AAATCCCTTAGGAGTTCTAA+AGG | - | chr7.3:78632684-78632703 | MS.gene97300:intron | 35.0% |
| ! | GTTATCAGACAATGTTGACT+TGG | - | chr7.3:78633962-78633981 | MS.gene97300:CDS | 35.0% |
| !! | AAACAAGTGCTGATCAAGAT+CGG | + | chr7.3:78633791-78633810 | None:intergenic | 35.0% |
| AAACCGTTCTCAAATCCCTT+AGG | - | chr7.3:78632673-78632692 | MS.gene97300:intron | 40.0% | |
| ACTCCTAAGGGATTTGAGAA+CGG | + | chr7.3:78632679-78632698 | None:intergenic | 40.0% | |
| ATTTCAGACTTCCTCAAAGG+AGG | + | chr7.3:78633025-78633044 | None:intergenic | 40.0% | |
| GAAGCTTACAGTCTTCTTTG+CGG | + | chr7.3:78632489-78632508 | None:intergenic | 40.0% | |
| GACATCATTAAGACAGTTCC+TGG | - | chr7.3:78634131-78634150 | MS.gene97300:intron | 40.0% | |
| GTTGGAGTTCACCATTACAA+TGG | - | chr7.3:78633070-78633089 | MS.gene97300:intron | 40.0% | |
| TGAACAACACGGAGAAGAAT+AGG | + | chr7.3:78633122-78633141 | None:intergenic | 40.0% | |
| ! | GTACTATGCTATGTTGGCTA+AGG | - | chr7.3:78633048-78633067 | MS.gene97300:intron | 40.0% |
| ! | TATGCTATGTTGGCTAAGGT+TGG | - | chr7.3:78633052-78633071 | MS.gene97300:intron | 40.0% |
| !! | GAGAACGGTTTTATACCCAA+GGG | + | chr7.3:78632664-78632683 | None:intergenic | 40.0% |
| !! | TGAGAACGGTTTTATACCCA+AGG | + | chr7.3:78632665-78632684 | None:intergenic | 40.0% |
| ATGTGATACATCGTACCCTG+TGG | - | chr7.3:78633486-78633505 | MS.gene97300:intron | 45.0% | |
| CTAACAACTGTCCTCCTTTG+AGG | - | chr7.3:78633011-78633030 | MS.gene97300:intron | 45.0% | |
| GAGAAGAATAGGAGCTAGAC+TGG | + | chr7.3:78633111-78633130 | None:intergenic | 45.0% | |
| TACCAGGATCAACAATGCTG+AGG | + | chr7.3:78634022-78634041 | None:intergenic | 45.0% | |
| !! | CTCATGAGAGCATCAACAAC+AGG | - | chr7.3:78632607-78632626 | MS.gene97300:CDS | 45.0% |
| !!! | GCTGATGGGCATGTTTTGAT+TGG | - | chr7.3:78633577-78633596 | MS.gene97300:CDS | 45.0% |
| AACTGACACTACTGCACCAC+AGG | + | chr7.3:78633505-78633524 | None:intergenic | 50.0% | |
| ACTGACACTACTGCACCACA+GGG | + | chr7.3:78633504-78633523 | None:intergenic | 50.0% | |
| CTCGCTCTTGTCATGAAGAG+TGG | - | chr7.3:78632630-78632649 | MS.gene97300:CDS | 50.0% | |
| TGCCTCAGCATTGTTGATCC+TGG | - | chr7.3:78634017-78634036 | MS.gene97300:CDS | 50.0% | |
| !! | GTTGACTTGGGCACTGCTTG+CGG | - | chr7.3:78633975-78633994 | MS.gene97300:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 78632479 | 78634163 | 78632479 | ID=MS.gene97300 |
| chr7.3 | mRNA | 78632479 | 78634163 | 78632479 | ID=MS.gene97300.t1;Parent=MS.gene97300 |
| chr7.3 | exon | 78634143 | 78634163 | 78634143 | ID=MS.gene97300.t1.exon1;Parent=MS.gene97300.t1 |
| chr7.3 | CDS | 78634143 | 78634163 | 78634143 | ID=cds.MS.gene97300.t1;Parent=MS.gene97300.t1 |
| chr7.3 | exon | 78633937 | 78634042 | 78633937 | ID=MS.gene97300.t1.exon2;Parent=MS.gene97300.t1 |
| chr7.3 | CDS | 78633937 | 78634042 | 78633937 | ID=cds.MS.gene97300.t1;Parent=MS.gene97300.t1 |
| chr7.3 | exon | 78633548 | 78633649 | 78633548 | ID=MS.gene97300.t1.exon3;Parent=MS.gene97300.t1 |
| chr7.3 | CDS | 78633548 | 78633649 | 78633548 | ID=cds.MS.gene97300.t1;Parent=MS.gene97300.t1 |
| chr7.3 | exon | 78632604 | 78632672 | 78632604 | ID=MS.gene97300.t1.exon4;Parent=MS.gene97300.t1 |
| chr7.3 | CDS | 78632604 | 78632672 | 78632604 | ID=cds.MS.gene97300.t1;Parent=MS.gene97300.t1 |
| chr7.3 | exon | 78632479 | 78632519 | 78632479 | ID=MS.gene97300.t1.exon5;Parent=MS.gene97300.t1 |
| chr7.3 | CDS | 78632479 | 78632519 | 78632479 | ID=cds.MS.gene97300.t1;Parent=MS.gene97300.t1 |
| Gene Sequence |
| Protein sequence |
