Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23883.t1 | XP_003621633.1 | 98.2 | 112 | 2 | 0 | 1 | 112 | 1 | 112 | 1.30E-56 | 228.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23883.t1 | O49884 | 98.2 | 112 | 2 | 0 | 1 | 112 | 1 | 112 | 5.1e-59 | 228.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23883.t1 | G7KSW7 | 98.2 | 112 | 2 | 0 | 1 | 112 | 1 | 112 | 9.0e-57 | 228.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene23883 | 0.843492 | 1.36E-58 | -1.69E-46 |
| MS.gene060856 | MS.gene23883 | 0.812932 | 3.31E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene97300 | MS.gene23883 | PPI |
| MS.gene022106 | MS.gene23883 | PPI |
| MS.gene23883 | MS.gene43173 | PPI |
| MS.gene074262 | MS.gene23883 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23883.t1 | MTR_7g021030 | 98.214 | 112 | 2 | 0 | 1 | 112 | 1 | 112 | 9.37e-79 | 227 |
| MS.gene23883.t1 | MTR_0024s0220 | 96.429 | 112 | 4 | 0 | 1 | 112 | 1 | 112 | 1.82e-77 | 224 |
| MS.gene23883.t1 | MTR_5g088740 | 91.964 | 112 | 9 | 0 | 1 | 112 | 1 | 112 | 3.35e-74 | 215 |
| MS.gene23883.t1 | MTR_0024s0220 | 96.040 | 101 | 4 | 0 | 1 | 101 | 1 | 101 | 2.35e-68 | 201 |
| MS.gene23883.t1 | MTR_7g021030 | 96.203 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 3.58e-50 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23883.t1 | AT1G36240 | 87.500 | 112 | 14 | 0 | 1 | 112 | 1 | 112 | 4.45e-71 | 207 |
| MS.gene23883.t1 | AT3G18740 | 86.607 | 112 | 15 | 0 | 1 | 112 | 1 | 112 | 3.39e-70 | 205 |
| MS.gene23883.t1 | AT1G77940 | 85.714 | 112 | 16 | 0 | 1 | 112 | 1 | 112 | 3.78e-70 | 205 |
Find 26 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAACAACTGTCCTCCTTTG+AGG | 0.365472 | 7.1:-77352271 | MS.gene23883:CDS |
| GACATCATCAAGACAGTTCC+TGG | 0.394800 | 7.1:-77350995 | MS.gene23883:CDS |
| ACAACTTACTTCCATTGTAA+TGG | 0.429111 | 7.1:+77352201 | None:intergenic |
| CTTTGAGTTTACTGATCTCC+AGG | 0.439455 | 7.1:+77350977 | None:intergenic |
| TGCCTCAGCATTCTTGATCC+TGG | 0.454752 | 7.1:-77351112 | MS.gene23883:intron |
| CTCATGAGAGCATCAACAAC+AGG | 0.462326 | 7.1:-77352666 | MS.gene23883:CDS |
| AATTACCTTTGGAACTCCTA+AGG | 0.463721 | 7.1:+77352584 | None:intergenic |
| TAAGCTTACGGTCTTCTTTG+CGG | 0.468667 | 7.1:+77352787 | None:intergenic |
| ATTACCTTTGGAACTCCTAA+GGG | 0.478678 | 7.1:+77352585 | None:intergenic |
| AAGAGTGGAAAATATACCCT+TGG | 0.505811 | 7.1:-77352628 | MS.gene23883:CDS |
| GTTGACCTTGGCACTGCTTG+TGG | 0.506943 | 7.1:-77351154 | MS.gene23883:CDS |
| AAACCGTTCTCAAATCCCTT+AGG | 0.509696 | 7.1:-77352600 | MS.gene23883:CDS |
| TATGCTATGTTGGCTAAGGT+TGG | 0.529938 | 7.1:-77352230 | MS.gene23883:CDS |
| ATCATAGACAATGTTGACCT+TGG | 0.543023 | 7.1:-77351166 | MS.gene23883:intron |
| CTCGCTCTTGTCATGAAGAG+TGG | 0.545906 | 7.1:-77352643 | MS.gene23883:CDS |
| AATTGAGTACTATGCTATGT+TGG | 0.552970 | 7.1:-77352240 | MS.gene23883:CDS |
| AGAGTGGAAAATATACCCTT+GGG | 0.553555 | 7.1:-77352627 | MS.gene23883:CDS |
| GTACTATGCTATGTTGGCTA+AGG | 0.556485 | 7.1:-77352234 | MS.gene23883:CDS |
| ATTTCAGACTTCCTCAAAGG+AGG | 0.587118 | 7.1:+77352260 | None:intergenic |
| ACTCCTAAGGGATTTGAGAA+CGG | 0.591174 | 7.1:+77352597 | None:intergenic |
| TCAATTTCAGACTTCCTCAA+AGG | 0.597003 | 7.1:+77352257 | None:intergenic |
| AGGAACTGTCTTGATGATGT+CGG | 0.599851 | 7.1:+77350997 | None:intergenic |
| GTTGGAGTTCACCATTACAA+TGG | 0.600433 | 7.1:-77352212 | MS.gene23883:CDS |
| AAATCCCTTAGGAGTTCCAA+AGG | 0.605938 | 7.1:-77352589 | MS.gene23883:intron |
| ACTTTCCACAAGCAGTGCCA+AGG | 0.620377 | 7.1:+77351149 | None:intergenic |
| TACCAGGATCAAGAATGCTG+AGG | 0.680906 | 7.1:+77351110 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACTGAAAAAATATTATACA+TGG | - | chr7.1:77352016-77352035 | MS.gene23883:intron | 15.0% |
| !!! | AACTTTATTTTATAAGATTC+TGG | - | chr7.1:77352091-77352110 | MS.gene23883:intron | 15.0% |
| !!! | CTCTAATTTTATTTGTTATT+TGG | - | chr7.1:77352744-77352763 | MS.gene23883:intron | 15.0% |
| !! | ATACTTAATACACAACAAAA+TGG | + | chr7.1:77351230-77351249 | None:intergenic | 20.0% |
| !! | ATGTTTGGATTAATGATATA+GGG | + | chr7.1:77351858-77351877 | None:intergenic | 20.0% |
| !! | ATTGAAAAATTAATGACACA+GGG | + | chr7.1:77351623-77351642 | None:intergenic | 20.0% |
| !! | GGATAAAAAAAAATTACCTT+TGG | + | chr7.1:77351209-77351228 | None:intergenic | 20.0% |
| !! | TATAATTATCTATCAGTAGT+TGG | - | chr7.1:77352196-77352215 | MS.gene23883:intron | 20.0% |
| !! | TATGTTTGGATTAATGATAT+AGG | + | chr7.1:77351859-77351878 | None:intergenic | 20.0% |
| !! | TGTTGAAAGAATATTATACA+TGG | - | chr7.1:77351962-77351981 | MS.gene23883:intron | 20.0% |
| !! | TTAATCCAAACATAATGTTA+TGG | - | chr7.1:77351865-77351884 | MS.gene23883:intron | 20.0% |
| !!! | AATATTTTTTCAGTATGACA+GGG | + | chr7.1:77352011-77352030 | None:intergenic | 20.0% |
| !!! | AGCTAGTTATACTTTAAAAA+TGG | - | chr7.1:77351801-77351820 | MS.gene23883:intron | 20.0% |
| !!! | TAATATTTTTTCAGTATGAC+AGG | + | chr7.1:77352012-77352031 | None:intergenic | 20.0% |
| !!! | TTATTTGATGATTATGCATA+TGG | - | chr7.1:77352323-77352342 | MS.gene23883:intron | 20.0% |
| !!! | TTGATTGAATTAAGTGATTT+TGG | - | chr7.1:77351409-77351428 | MS.gene23883:intron | 20.0% |
| !!! | TTTGATTTGATTATTCTGTA+GGG | - | chr7.1:77351469-77351488 | MS.gene23883:intron | 20.0% |
| !!! | TTTGTTGTGTATTAAGTATT+GGG | - | chr7.1:77351231-77351250 | MS.gene23883:intron | 20.0% |
| !!! | TTTTGATTTGATTATTCTGT+AGG | - | chr7.1:77351468-77351487 | MS.gene23883:intron | 20.0% |
| !!! | TTTTGTTGTGTATTAAGTAT+TGG | - | chr7.1:77351230-77351249 | MS.gene23883:intron | 20.0% |
| ! | AAATAAGTGATTAGTGAAAG+TGG | - | chr7.1:77351363-77351382 | MS.gene23883:intron | 25.0% |
| ! | ATGATTTCGTTTCAGATTTA+GGG | - | chr7.1:77351044-77351063 | MS.gene23883:intron | 25.0% |
| ! | CATTGAAAAATTAATGACAC+AGG | + | chr7.1:77351624-77351643 | None:intergenic | 25.0% |
| ! | TAGAATGAAATGAAATGTAG+TGG | - | chr7.1:77351761-77351780 | MS.gene23883:intron | 25.0% |
| ! | TATGATTTCGTTTCAGATTT+AGG | - | chr7.1:77351043-77351062 | MS.gene23883:intron | 25.0% |
| !! | TACATTAACAGAAGCATATT+TGG | + | chr7.1:77352254-77352273 | None:intergenic | 25.0% |
| !! | TAGTTTGATGATTATGCATA+TGG | - | chr7.1:77352348-77352367 | MS.gene23883:intron | 25.0% |
| !!! | ATTTGTTATTTGGTGTTTTC+AGG | - | chr7.1:77352754-77352773 | MS.gene23883:intron | 25.0% |
| !!! | TGAAATGTAGTGGATTAAAA+TGG | - | chr7.1:77351771-77351790 | MS.gene23883:intron | 25.0% |
| AATTATTGCATGTGACCATA+AGG | + | chr7.1:77351918-77351937 | None:intergenic | 30.0% | |
| AATTGAGTACTATGCTATGT+TGG | - | chr7.1:77351539-77351558 | MS.gene23883:intron | 30.0% | |
| ACAACTTACTTCCATTGTAA+TGG | + | chr7.1:77351581-77351600 | None:intergenic | 30.0% | |
| ATTATTGCATGTGACCATAA+GGG | + | chr7.1:77351917-77351936 | None:intergenic | 30.0% | |
| ATTGTGTATGTATACCCTTA+TGG | - | chr7.1:77351900-77351919 | MS.gene23883:intron | 30.0% | |
| TAAAGAAAGCAACATATACC+AGG | + | chr7.1:77352688-77352707 | None:intergenic | 30.0% | |
| TCAATGTTGATATGCATTCA+GGG | - | chr7.1:77351638-77351657 | MS.gene23883:intron | 30.0% | |
| TGTGACCATAACATTATGTT+TGG | + | chr7.1:77351873-77351892 | None:intergenic | 30.0% | |
| TTCAATGTTGATATGCATTC+AGG | - | chr7.1:77351637-77351656 | MS.gene23883:intron | 30.0% | |
| TTTGGATTAATGATATAGGG+TGG | + | chr7.1:77351855-77351874 | None:intergenic | 30.0% | |
| !! | GTTGTAGATTGATTTTGTAG+TGG | - | chr7.1:77351340-77351359 | MS.gene23883:intron | 30.0% |
| !! | TATGAGTTGATTAGATTGCA+AGG | - | chr7.1:77351276-77351295 | MS.gene23883:intron | 30.0% |
| !! | TTTGAATGTTGATTTGGTAG+TGG | - | chr7.1:77351435-77351454 | MS.gene23883:intron | 30.0% |
| !!! | TGGAGTTTTGAATGTTGATT+TGG | - | chr7.1:77351429-77351448 | MS.gene23883:intron | 30.0% |
| AAGAGTGGAAAATATACCCT+TGG | - | chr7.1:77351151-77351170 | MS.gene23883:CDS | 35.0% | |
| AGAGTGGAAAATATACCCTT+GGG | - | chr7.1:77351152-77351171 | MS.gene23883:CDS | 35.0% | |
| ATCATAGACAATGTTGACCT+TGG | - | chr7.1:77352613-77352632 | MS.gene23883:CDS | 35.0% | |
| TAAGAAGAATCCCAACGATT+TGG | + | chr7.1:77352448-77352467 | None:intergenic | 35.0% | |
| TATCTATCAGTAGTTGGATG+TGG | - | chr7.1:77352202-77352221 | MS.gene23883:intron | 35.0% | |
| TATGCATTCAGGGATTGTTT+AGG | - | chr7.1:77351648-77351667 | MS.gene23883:intron | 35.0% | |
| TCAATTTCAGACTTCCTCAA+AGG | + | chr7.1:77351525-77351544 | None:intergenic | 35.0% | |
| TGCTGAATGAGATAAGCTTA+CGG | + | chr7.1:77351007-77351026 | None:intergenic | 35.0% | |
| TTAATGACACAGGGAAGTAA+AGG | + | chr7.1:77351614-77351633 | None:intergenic | 35.0% | |
| ! | CAGAAGCATATTTGGAAACA+AGG | + | chr7.1:77352246-77352265 | None:intergenic | 35.0% |
| ! | TCTGTTATCAGTTGACTCTA+AGG | - | chr7.1:77352534-77352553 | MS.gene23883:intron | 35.0% |
| !! | AATTACCTTTGGAACTCCTA+AGG | + | chr7.1:77351198-77351217 | None:intergenic | 35.0% |
| !! | ATAGTAACAAGTGCTGATCA+AGG | + | chr7.1:77352404-77352423 | None:intergenic | 35.0% |
| !! | ATTACCTTTGGAACTCCTAA+GGG | + | chr7.1:77351197-77351216 | None:intergenic | 35.0% |
| !! | TGTTGTTTCTCCAAATCGTT+GGG | - | chr7.1:77352435-77352454 | MS.gene23883:intron | 35.0% |
| !! | TTGTTGTTTCTCCAAATCGT+TGG | - | chr7.1:77352434-77352453 | MS.gene23883:intron | 35.0% |
| AAACCGTTCTCAAATCCCTT+AGG | - | chr7.1:77351179-77351198 | MS.gene23883:intron | 40.0% | |
| AAATCCCTTAGGAGTTCCAA+AGG | - | chr7.1:77351190-77351209 | MS.gene23883:intron | 40.0% | |
| AAATGACACTACTGTACGAC+AGG | + | chr7.1:77352066-77352085 | None:intergenic | 40.0% | |
| AATGACACTACTGTACGACA+GGG | + | chr7.1:77352065-77352084 | None:intergenic | 40.0% | |
| ACTCCTAAGGGATTTGAGAA+CGG | + | chr7.1:77351185-77351204 | None:intergenic | 40.0% | |
| ATTTCAGACTTCCTCAAAGG+AGG | + | chr7.1:77351522-77351541 | None:intergenic | 40.0% | |
| ATTTGATCTTACGCTGCTGT+TGG | - | chr7.1:77352130-77352149 | MS.gene23883:intron | 40.0% | |
| GTTGGAGTTCACCATTACAA+TGG | - | chr7.1:77351567-77351586 | MS.gene23883:intron | 40.0% | |
| TAAGCTTACGGTCTTCTTTG+CGG | + | chr7.1:77350995-77351014 | None:intergenic | 40.0% | |
| TCAGCTAGCTAGATTCAATC+AGG | + | chr7.1:77351719-77351738 | None:intergenic | 40.0% | |
| TTTGATCTTACGCTGCTGTT+GGG | - | chr7.1:77352131-77352150 | MS.gene23883:intron | 40.0% | |
| ! | GTACTATGCTATGTTGGCTA+AGG | - | chr7.1:77351545-77351564 | MS.gene23883:intron | 40.0% |
| ! | TATGCTATGTTGGCTAAGGT+TGG | - | chr7.1:77351549-77351568 | MS.gene23883:intron | 40.0% |
| ! | TCTGTTATCAGTTGACTCTG+AGG | - | chr7.1:77352495-77352514 | MS.gene23883:intron | 40.0% |
| ! | TGAGCTTTAGTGTGTATCAG+TGG | - | chr7.1:77351736-77351755 | MS.gene23883:intron | 40.0% |
| !! | AGGAACTGTCTTGATGATGT+CGG | + | chr7.1:77352785-77352804 | None:intergenic | 40.0% |
| !! | GAGAACGGTTTTATACCCAA+GGG | + | chr7.1:77351170-77351189 | None:intergenic | 40.0% |
| !! | TGAGAACGGTTTTATACCCA+AGG | + | chr7.1:77351171-77351190 | None:intergenic | 40.0% |
| CTAACAACTGTCCTCCTTTG+AGG | - | chr7.1:77351508-77351527 | MS.gene23883:intron | 45.0% | |
| GACATCATCAAGACAGTTCC+TGG | - | chr7.1:77352784-77352803 | MS.gene23883:intron | 45.0% | |
| TACCAGGATCAAGAATGCTG+AGG | + | chr7.1:77352672-77352691 | None:intergenic | 45.0% | |
| !! | CTCATGAGAGCATCAACAAC+AGG | - | chr7.1:77351113-77351132 | MS.gene23883:CDS | 45.0% |
| ACTTTCCACAAGCAGTGCCA+AGG | + | chr7.1:77352633-77352652 | None:intergenic | 50.0% | |
| CTCGCTCTTGTCATGAAGAG+TGG | - | chr7.1:77351136-77351155 | MS.gene23883:CDS | 50.0% | |
| TGCCTCAGCATTCTTGATCC+TGG | - | chr7.1:77352667-77352686 | MS.gene23883:CDS | 50.0% | |
| GTTGACCTTGGCACTGCTTG+TGG | - | chr7.1:77352625-77352644 | MS.gene23883:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 77350985 | 77352816 | 77350985 | ID=MS.gene23883 |
| chr7.1 | mRNA | 77350985 | 77352816 | 77350985 | ID=MS.gene23883.t1;Parent=MS.gene23883 |
| chr7.1 | exon | 77352796 | 77352816 | 77352796 | ID=MS.gene23883.t1.exon1;Parent=MS.gene23883.t1 |
| chr7.1 | CDS | 77352796 | 77352816 | 77352796 | ID=cds.MS.gene23883.t1;Parent=MS.gene23883.t1 |
| chr7.1 | exon | 77352590 | 77352695 | 77352590 | ID=MS.gene23883.t1.exon2;Parent=MS.gene23883.t1 |
| chr7.1 | CDS | 77352590 | 77352695 | 77352590 | ID=cds.MS.gene23883.t1;Parent=MS.gene23883.t1 |
| chr7.1 | exon | 77352210 | 77352311 | 77352210 | ID=MS.gene23883.t1.exon3;Parent=MS.gene23883.t1 |
| chr7.1 | CDS | 77352210 | 77352311 | 77352210 | ID=cds.MS.gene23883.t1;Parent=MS.gene23883.t1 |
| chr7.1 | exon | 77351113 | 77351181 | 77351113 | ID=MS.gene23883.t1.exon4;Parent=MS.gene23883.t1 |
| chr7.1 | CDS | 77351113 | 77351181 | 77351113 | ID=cds.MS.gene23883.t1;Parent=MS.gene23883.t1 |
| chr7.1 | exon | 77350985 | 77351025 | 77350985 | ID=MS.gene23883.t1.exon5;Parent=MS.gene23883.t1 |
| chr7.1 | CDS | 77350985 | 77351025 | 77350985 | ID=cds.MS.gene23883.t1;Parent=MS.gene23883.t1 |
| Gene Sequence |
| Protein sequence |
