Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97547.t1 | XP_003621906.2 | 93.4 | 137 | 9 | 0 | 1 | 137 | 34 | 170 | 3.40E-64 | 254.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97547.t1 | G7KXV9 | 93.4 | 137 | 9 | 0 | 1 | 137 | 1 | 137 | 2.5e-64 | 254.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052606 | MS.gene97547 | 0.806315 | 8.73E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene97547 | 0.805031 | 1.62E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene97547 | 0.812766 | 3.59E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene97547 | 0.803839 | 2.88E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97547.t1 | MTR_7g024880 | 93.431 | 137 | 9 | 0 | 1 | 137 | 1 | 137 | 1.34e-90 | 259 |
| MS.gene97547.t1 | MTR_7g024890 | 93.431 | 137 | 9 | 0 | 1 | 137 | 1 | 137 | 1.58e-90 | 258 |
| MS.gene97547.t1 | MTR_7g024873 | 91.971 | 137 | 11 | 0 | 1 | 137 | 1 | 137 | 2.80e-90 | 258 |
| MS.gene97547.t1 | MTR_8g446810 | 48.571 | 140 | 69 | 1 | 1 | 137 | 1 | 140 | 6.53e-41 | 133 |
| MS.gene97547.t1 | MTR_7g024900 | 88.235 | 51 | 6 | 0 | 1 | 51 | 1 | 51 | 1.15e-25 | 92.0 |
| MS.gene97547.t1 | MTR_1g081270 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 2.96e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97547.t1 | AT2G44310 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 5.37e-39 | 129 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGATATTT+TGG | 0.168880 | 7.2:+74771483 | None:intergenic |
| CTTCTTCATCTTCAAGGAAA+TGG | 0.295262 | 7.2:-74771522 | None:intergenic |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7.2:-74771685 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.342383 | 7.2:-74771528 | None:intergenic |
| CTTGCAATGGCAGATGGGTT+GGG | 0.381320 | 7.2:+74771793 | MS.gene97547:CDS |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7.2:+74771819 | MS.gene97547:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7.2:-74771823 | None:intergenic |
| TTAATAGCTTGGTAGGTAAT+CGG | 0.402120 | 7.2:+74771548 | MS.gene97547:CDS |
| CTTGAATGGCACAATTGATC+TGG | 0.437675 | 7.2:+74771738 | MS.gene97547:CDS |
| GGGTTTCATGCCAATTCAAA+TGG | 0.441784 | 7.2:+74771813 | MS.gene97547:CDS |
| TGAAGAAGCATTTAATAGCT+TGG | 0.442582 | 7.2:+74771537 | MS.gene97547:CDS |
| CTCCTTCAACATCTCTTCAT+AGG | 0.458078 | 7.2:-74771608 | None:intergenic |
| GCTTGCAATGGCAGATGGGT+TGG | 0.461788 | 7.2:+74771792 | MS.gene97547:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7.2:-74771684 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7.2:+74771624 | MS.gene97547:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7.2:+74771724 | MS.gene97547:CDS |
| CTCCTATGAAGAGATGTTGA+AGG | 0.486473 | 7.2:+74771606 | MS.gene97547:CDS |
| GAAGCATTTAATAGCTTGGT+AGG | 0.494927 | 7.2:+74771541 | MS.gene97547:CDS |
| TTTCGAAAACCCTCAAGCTC+CGG | 0.509695 | 7.2:-74771633 | None:intergenic |
| AACACACTTTGGTATTGATG+TGG | 0.520192 | 7.2:+74771654 | MS.gene97547:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7.2:+74771623 | MS.gene97547:CDS |
| AAGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7.2:+74771614 | MS.gene97547:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7.2:+74771870 | MS.gene97547:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558625 | 7.2:+74771787 | MS.gene97547:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.574792 | 7.2:-74771579 | None:intergenic |
| TTCAAGGAAATGGAAAATTG+TGG | 0.612346 | 7.2:-74771512 | None:intergenic |
| TAAGCAGATGATGCTTGCAA+TGG | 0.622742 | 7.2:+74771780 | MS.gene97547:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.629974 | 7.2:+74771788 | MS.gene97547:CDS |
| CGCCTCGACACAAACAACGA+CGG | 0.676658 | 7.2:+74771577 | MS.gene97547:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7.2:74771531-74771550 | None:intergenic | 30.0% | |
| TTAATAGCTTGGTAGGTAAT+CGG | + | chr7.2:74771548-74771567 | MS.gene97547:CDS | 30.0% | |
| TTCAAGGAAATGGAAAATTG+TGG | - | chr7.2:74771515-74771534 | None:intergenic | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7.2:74771870-74771889 | MS.gene97547:CDS | 30.0% |
| !! | ATGAGTGTAGATATTTTGGA+TGG | + | chr7.2:74771487-74771506 | MS.gene97547:CDS | 30.0% |
| !! | TGAAGAAGCATTTAATAGCT+TGG | + | chr7.2:74771537-74771556 | MS.gene97547:CDS | 30.0% |
| !! | TGAGTGTAGATATTTTGGAT+GGG | + | chr7.2:74771488-74771507 | MS.gene97547:CDS | 30.0% |
| CTTCTTCATCTTCAAGGAAA+TGG | - | chr7.2:74771525-74771544 | None:intergenic | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7.2:74771826-74771845 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTTGGT+AGG | + | chr7.2:74771541-74771560 | MS.gene97547:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7.2:74771654-74771673 | MS.gene97547:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7.2:74771858-74771877 | None:intergenic | 35.0% |
| !! | GAGTGTAGATATTTTGGATG+GGG | + | chr7.2:74771489-74771508 | MS.gene97547:CDS | 35.0% |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7.2:74771724-74771743 | MS.gene97547:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7.2:74771819-74771838 | MS.gene97547:CDS | 40.0% | |
| CTCCTATGAAGAGATGTTGA+AGG | + | chr7.2:74771606-74771625 | MS.gene97547:CDS | 40.0% | |
| CTCCTTCAACATCTCTTCAT+AGG | - | chr7.2:74771611-74771630 | None:intergenic | 40.0% | |
| CTTGAATGGCACAATTGATC+TGG | + | chr7.2:74771738-74771757 | MS.gene97547:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7.2:74771813-74771832 | MS.gene97547:CDS | 40.0% | |
| TAAGCAGATGATGCTTGCAA+TGG | + | chr7.2:74771780-74771799 | MS.gene97547:CDS | 40.0% | |
| ! | AGGGTTTTCGAAACACACTT+TGG | + | chr7.2:74771643-74771662 | MS.gene97547:CDS | 40.0% |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7.2:74771687-74771706 | None:intergenic | 45.0% | |
| AAGAGATGTTGAAGGAGCTC+CGG | + | chr7.2:74771614-74771633 | MS.gene97547:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7.2:74771787-74771806 | MS.gene97547:CDS | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7.2:74771688-74771707 | None:intergenic | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7.2:74771788-74771807 | MS.gene97547:CDS | 45.0% | |
| TTTCGAAAACCCTCAAGCTC+CGG | - | chr7.2:74771636-74771655 | None:intergenic | 45.0% | |
| ! | CTTGCAATGGCAGATGGGTT+GGG | + | chr7.2:74771793-74771812 | MS.gene97547:CDS | 50.0% |
| CGCCTCGACACAAACAACGA+CGG | + | chr7.2:74771577-74771596 | MS.gene97547:CDS | 55.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | - | chr7.2:74771582-74771601 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGT+TGG | + | chr7.2:74771792-74771811 | MS.gene97547:CDS | 55.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7.2:74771624-74771643 | MS.gene97547:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7.2:74771623-74771642 | MS.gene97547:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 74771487 | 74771900 | 74771487 | ID=MS.gene97547 |
| chr7.2 | mRNA | 74771487 | 74771900 | 74771487 | ID=MS.gene97547.t1;Parent=MS.gene97547 |
| chr7.2 | exon | 74771487 | 74771900 | 74771487 | ID=MS.gene97547.t1.exon1;Parent=MS.gene97547.t1 |
| chr7.2 | CDS | 74771487 | 74771900 | 74771487 | ID=cds.MS.gene97547.t1;Parent=MS.gene97547.t1 |
| Gene Sequence |
| Protein sequence |
