AlfalfaGEDB | MS.gene98524

M. sativa cultivar XinJiangDaYe / MS.gene98524

KEGG: -
GO: -
PFAM: PF07172.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98524.t1	XP_003616815.1	90.1	142	5	2	4	139	38	176	7.80E-24	120.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98524.t1	G7K7E3	90.1	142	5	2	4	139	38	176	5.6e-24	120.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058201	MS.gene98524	0.80109	1.06E-48	-1.69E-46
MS.gene059894	MS.gene98524	0.810404	1.17E-50	-1.69E-46
MS.gene059899	MS.gene98524	0.801127	1.04E-48	-1.69E-46
MS.gene061112	MS.gene98524	0.806588	7.65E-50	-1.69E-46
MS.gene061265	MS.gene98524	0.804925	1.71E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98524.t1	MTR_5g084570	64.085	142	42	2	4	139	38	176	2.64e-20	82.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 58 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTAATTACCACCACCATATT+TGG	0.192353	5.2:+72219498	None:intergenic
ATTGTGGTAACCGCCATATT+TGG	0.317393	5.2:+72219696	None:intergenic
GAAGTAAACGATGCCAAATA+TGG	0.333960	5.2:-72219709	MS.gene98524:CDS
GAAGTAAACGATGCCAAATA+TGG	0.342429	5.2:-72219511	MS.gene98524:CDS
AATGGTGGTGGACATTACTA+TGG	0.429163	5.2:-72219472	MS.gene98524:CDS
AATGGTGGTGGACATTACTA+TGG	0.429163	5.2:-72219655	MS.gene98524:CDS
GGTGGTTCCCACCACGGTGG+AGG	0.469523	5.2:-72219613	MS.gene98524:CDS
GGTGGTTCTCACCATGGTGG+AGG	0.471933	5.2:-72219427	MS.gene98524:CDS
GGTGGTTCTCACGGTGGAGG+TGG	0.475576	5.2:-72219631	MS.gene98524:CDS
GGTTCTCACCATGGTGGAGG+TGG	0.480643	5.2:-72219424	MS.gene98524:CDS
GGTTCCCACCACGGTGGAGG+TGG	0.484027	5.2:-72219610	MS.gene98524:CDS
GGTGGTTCTCACCACGGTGG+AGG	0.484853	5.2:-72219448	MS.gene98524:CDS
GGAGGTGGTTCTCACGGTGG+AGG	0.512830	5.2:-72219634	MS.gene98524:CDS
GGTTCTCACCACGGTGGAGG+TGG	0.520241	5.2:-72219445	MS.gene98524:CDS
GCAACCACCTCCACCGTGGT+GGG	0.523248	5.2:+72219606	None:intergenic
TACTATGGAGGTGGTTCTCA+CGG	0.525230	5.2:-72219640	MS.gene98524:CDS
ATTCAGCATGTGAGCAGCAA+TGG	0.536032	5.2:+72219380	None:intergenic
GGTAATTACCACAATGGTGG+TGG	0.547595	5.2:-72219484	MS.gene98524:CDS
GGTAATTACCACAATGGTGG+TGG	0.547595	5.2:-72219667	MS.gene98524:CDS
CATGTGAGCAGCAATGGCCG+TGG	0.551382	5.2:+72219386	None:intergenic
GGCGGTTACCACAATGGTGG+TGG	0.554658	5.2:-72219688	MS.gene98524:CDS
GGTGGACATTACTATGGAGG+TGG	0.556814	5.2:-72219466	MS.gene98524:CDS
GGTGGACATTACTATGGAGG+TGG	0.556814	5.2:-72219649	MS.gene98524:CDS
CATATGAGCAGCAATGGCCG+TGG	0.561023	5.2:+72219572	None:intergenic
CATATATATTATGTTTGCAG+AGG	0.566274	5.2:-72219749	None:intergenic
AACGATGCCAAATATGGTGG+TGG	0.579616	5.2:-72219505	MS.gene98524:CDS
GGCAACCACCTCCACCGTGG+TGG	0.590734	5.2:+72219605	None:intergenic
GTTTGTTGCCGTGCAAGCTG+AGG	0.597485	5.2:-72219545	MS.gene98524:CDS
GGTTGCCATTACTATTGCCA+CGG	0.599690	5.2:-72219403	MS.gene98524:CDS
AATGGCCGTGGCAATAGTAA+TGG	0.601894	5.2:+72219398	None:intergenic
AAATATGGCGGTTACCACAA+TGG	0.601953	5.2:-72219694	MS.gene98524:CDS
ACTCGGCATATGAGCAGCAA+TGG	0.602148	5.2:+72219566	None:intergenic
AGCTTGCACGGCAACAAACT+CGG	0.602417	5.2:+72219549	None:intergenic
GGTAATTACCACCACCATTG+TGG	0.604720	5.2:+72219680	None:intergenic
GGTGGAGGTGGTTCCCACCA+CGG	0.608637	5.2:-72219619	MS.gene98524:CDS
GGTGGAGGTGGTTCTCACCA+TGG	0.610718	5.2:-72219433	MS.gene98524:CDS
GGTTGCCATCACTATTGCCA+CGG	0.613013	5.2:-72219589	MS.gene98524:CDS
AATGGCCGTGGCAATAGTGA+TGG	0.615442	5.2:+72219584	None:intergenic
AGTAATGTCCACCACCATTG+TGG	0.641701	5.2:+72219476	None:intergenic
AGTAATGTCCACCACCATTG+TGG	0.641701	5.2:+72219659	None:intergenic
TATGGAGGTGGTTCTCACCA+CGG	0.648357	5.2:-72219454	MS.gene98524:CDS
GGAGGTGGTTCTCACCATGG+TGG	0.655820	5.2:-72219430	MS.gene98524:CDS
GGAGGTGGTTCCCACCACGG+TGG	0.673714	5.2:-72219616	MS.gene98524:CDS
ATTTGTTGCCGTGCAAGCTG+AGG	0.673816	5.2:-72219359	MS.gene98524:CDS
AATGGCAACCACCTCCACCA+TGG	0.681347	5.2:+72219416	None:intergenic
GGTGGTAATTACCACAATGG+TGG	0.685453	5.2:-72219487	MS.gene98524:CDS
GGTGGTAATTACCACAATGG+TGG	0.685453	5.2:-72219670	MS.gene98524:CDS
GGTGGTGGACATTACTATGG+AGG	0.685934	5.2:-72219469	MS.gene98524:CDS
GGTGGTGGACATTACTATGG+AGG	0.685934	5.2:-72219652	MS.gene98524:CDS
GTAAACGATGCCAAATATGG+CGG	0.688367	5.2:-72219706	MS.gene98524:CDS
TATGGAGGTGGTTCTCACGG+TGG	0.695015	5.2:-72219637	MS.gene98524:CDS
TATGGCGGTTACCACAATGG+TGG	0.720585	5.2:-72219691	MS.gene98524:CDS
GGTGGTGGTAATTACCACAA+TGG	0.736293	5.2:-72219490	MS.gene98524:CDS
GGTGGTGGTAATTACCACAA+TGG	0.736293	5.2:-72219673	MS.gene98524:CDS
GTAAACGATGCCAAATATGG+TGG	0.739461	5.2:-72219508	MS.gene98524:CDS
GGAGGTGGTTCTCACCACGG+TGG	0.747901	5.2:-72219451	MS.gene98524:CDS
GATGGCAACCACCTCCACCG+TGG	0.754549	5.2:+72219602	None:intergenic
GGTGAGAACCACCTCCACCG+TGG	0.755651	5.2:+72219437	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAGTAAACGATGCCAAATA+TGG	-	chr5.2:72219372-72219391	MS.gene98524:CDS	35.0%
	GTAATTACCACCACCATATT+TGG	+	chr5.2:72219586-72219605	None:intergenic	35.0%
	AAATATGGCGGTTACCACAA+TGG	-	chr5.2:72219387-72219406	MS.gene98524:CDS	40.0%
	ATTGTGGTAACCGCCATATT+TGG	+	chr5.2:72219388-72219407	None:intergenic	40.0%
	GTAAACGATGCCAAATATGG+CGG	-	chr5.2:72219375-72219394	MS.gene98524:CDS	40.0%
	GTAAACGATGCCAAATATGG+TGG	-	chr5.2:72219375-72219394	MS.gene98524:CDS	40.0%
!!	AATGGTGGTGGACATTACTA+TGG	-	chr5.2:72219426-72219445	MS.gene98524:CDS	40.0%
	AACGATGCCAAATATGGTGG+TGG	-	chr5.2:72219576-72219595	MS.gene98524:CDS	45.0%
	AATGGCCGTGGCAATAGTAA+TGG	+	chr5.2:72219686-72219705	None:intergenic	45.0%
	AGTAATGTCCACCACCATTG+TGG	+	chr5.2:72219425-72219444	None:intergenic	45.0%
	ATTCAGCATGTGAGCAGCAA+TGG	+	chr5.2:72219704-72219723	None:intergenic	45.0%
	GGTAATTACCACAATGGTGG+TGG	-	chr5.2:72219414-72219433	MS.gene98524:CDS	45.0%
	GGTAATTACCACCACCATTG+TGG	+	chr5.2:72219404-72219423	None:intergenic	45.0%
	GGTGGTAATTACCACAATGG+TGG	-	chr5.2:72219411-72219430	MS.gene98524:CDS	45.0%
	GGTTGCCATTACTATTGCCA+CGG	-	chr5.2:72219678-72219697	MS.gene98524:CDS	45.0%
	TACTATGGAGGTGGTTCTCA+CGG	-	chr5.2:72219441-72219460	MS.gene98524:CDS	45.0%
!	AGTCTTTTCCTCAGCTTGCA+CGG	+	chr5.2:72219547-72219566	None:intergenic	45.0%
!	GGTGGTGGTAATTACCACAA+TGG	-	chr5.2:72219408-72219427	MS.gene98524:CDS	45.0%
!!	AGTTTTGTCCTCAGCTTGCA+CGG	+	chr5.2:72219733-72219752	None:intergenic	45.0%
	AATGGCCGTGGCAATAGTGA+TGG	+	chr5.2:72219500-72219519	None:intergenic	50.0%
	ACTCGGCATATGAGCAGCAA+TGG	+	chr5.2:72219518-72219537	None:intergenic	50.0%
	AGCTTGCACGGCAACAAACT+CGG	+	chr5.2:72219535-72219554	None:intergenic	50.0%
	GGTGGACATTACTATGGAGG+TGG	-	chr5.2:72219432-72219451	MS.gene98524:CDS	50.0%
	GGTTGCCATCACTATTGCCA+CGG	-	chr5.2:72219492-72219511	MS.gene98524:CDS	50.0%
	TATGGAGGTGGTTCTCACCA+CGG	-	chr5.2:72219627-72219646	MS.gene98524:CDS	50.0%
	TATGGCGGTTACCACAATGG+TGG	-	chr5.2:72219390-72219409	MS.gene98524:CDS	50.0%
!	ATTTGTTGCCGTGCAAGCTG+AGG	-	chr5.2:72219722-72219741	MS.gene98524:CDS	50.0%
!!	GGTGGTGGACATTACTATGG+AGG	-	chr5.2:72219429-72219448	MS.gene98524:CDS	50.0%
	AATGGCAACCACCTCCACCA+TGG	+	chr5.2:72219668-72219687	None:intergenic	55.0%
	CATATGAGCAGCAATGGCCG+TGG	+	chr5.2:72219512-72219531	None:intergenic	55.0%
	TATGGAGGTGGTTCTCACGG+TGG	-	chr5.2:72219444-72219463	MS.gene98524:CDS	55.0%
!	GTTTGTTGCCGTGCAAGCTG+AGG	-	chr5.2:72219536-72219555	MS.gene98524:CDS	55.0%
	CATGTGAGCAGCAATGGCCG+TGG	+	chr5.2:72219698-72219717	None:intergenic	60.0%
	GGAGGTGGTTCTCACCATGG+TGG	-	chr5.2:72219651-72219670	MS.gene98524:CDS	60.0%
	GGCGGTTACCACAATGGTGG+TGG	-	chr5.2:72219393-72219412	MS.gene98524:CDS	60.0%
	GGTGGAGGTGGTTCTCACCA+TGG	-	chr5.2:72219648-72219667	MS.gene98524:CDS	60.0%
	GGTGGTTCTCACCATGGTGG+AGG	-	chr5.2:72219654-72219673	MS.gene98524:CDS	60.0%
!	GGTTCTCACCATGGTGGAGG+TGG	-	chr5.2:72219657-72219676	MS.gene98524:CDS	60.0%
	GATGGCAACCACCTCCACCG+TGG	+	chr5.2:72219482-72219501	None:intergenic	65.0%
	GCAACCACCTCCACCGTGGT+GGG	+	chr5.2:72219478-72219497	None:intergenic	65.0%
	GGAGGTGGTTCTCACCACGG+TGG	-	chr5.2:72219630-72219649	MS.gene98524:CDS	65.0%
	GGAGGTGGTTCTCACGGTGG+AGG	-	chr5.2:72219447-72219466	MS.gene98524:CDS	65.0%
	GGTGAGAACCACCTCCACCG+TGG	+	chr5.2:72219647-72219666	None:intergenic	65.0%
	GGTGGAGGTGGTTCCCACCA+CGG	-	chr5.2:72219462-72219481	MS.gene98524:CDS	65.0%
	GGTGGTTCTCACCACGGTGG+AGG	-	chr5.2:72219633-72219652	MS.gene98524:CDS	65.0%
	GGTGGTTCTCACGGTGGAGG+TGG	-	chr5.2:72219450-72219469	MS.gene98524:CDS	65.0%
	GGTTCTCACCACGGTGGAGG+TGG	-	chr5.2:72219636-72219655	MS.gene98524:CDS	65.0%
	GGAGGTGGTTCCCACCACGG+TGG	-	chr5.2:72219465-72219484	MS.gene98524:CDS	70.0%
	GGCAACCACCTCCACCGTGG+TGG	+	chr5.2:72219479-72219498	None:intergenic	70.0%
	GGTGGTTCCCACCACGGTGG+AGG	-	chr5.2:72219468-72219487	MS.gene98524:CDS	70.0%
	GGTTCCCACCACGGTGGAGG+TGG	-	chr5.2:72219471-72219490	MS.gene98524:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	72219342	72219761	72219342	ID=MS.gene98524
chr5.2	mRNA	72219342	72219761	72219342	ID=MS.gene98524.t1;Parent=MS.gene98524
chr5.2	exon	72219342	72219761	72219342	ID=MS.gene98524.t1.exon1;Parent=MS.gene98524.t1
chr5.2	CDS	72219342	72219761	72219342	ID=cds.MS.gene98524.t1;Parent=MS.gene98524.t1

Gene Sequence

>MS.gene98524

ATGTTTGCAGAGGTTGTTGAAAAGACTAATGAAGTAAACGATGCCAAATATGGCGGTTACCACAATGGTGGTGGTAATTACCACAATGGTGGTGGACATTACTATGGAGGTGGTTCTCACGGTGGAGGTGGTTCCCACCACGGTGGAGGTGGTTGCCATCACTATTGCCACGGCCATTGCTGCTCATATGCCGAGTTTGTTGCCGTGCAAGCTGAGGAAAAGACTAATGAAGTAAACGATGCCAAATATGGTGGTGGTAATTACCACAATGGTGGTGGACATTACTATGGAGGTGGTTCTCACCACGGTGGAGGTGGTTCTCACCATGGTGGAGGTGGTTGCCATTACTATTGCCACGGCCATTGCTGCTCACATGCTGAATTTGTTGCCGTGCAAGCTGAGGACAAAACTCAGAACTGA

Protein sequence

>MS.gene98524.t1

MFAEVVEKTNEVNDAKYGGYHNGGGNYHNGGGHYYGGGSHGGGGSHHGGGGCHHYCHGHCCSYAEFVAVQAEEKTNEVNDAKYGGGNYHNGGGHYYGGGSHHGGGGSHHGGGGCHYYCHGHCCSHAEFVAVQAEDKTQN