Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99342.t1 | KEH16787.1 | 96 | 174 | 7 | 0 | 31 | 204 | 153 | 326 | 8.80E-88 | 333.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99342.t1 | A0A072THA4 | 96.0 | 174 | 7 | 0 | 31 | 204 | 153 | 326 | 6.4e-88 | 333.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050273 | MS.gene99342 | -0.80294 | 4.42E-49 | -1.69E-46 |
| MS.gene052239 | MS.gene99342 | 0.800995 | 1.11E-48 | -1.69E-46 |
| MS.gene053931 | MS.gene99342 | -0.818677 | 1.73E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene99342 | -0.840032 | 1.11E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99342.t1 | MTR_0094s0120 | 94.886 | 176 | 9 | 0 | 31 | 206 | 153 | 328 | 3.44e-119 | 343 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTTTTTTCTTCCTTATTT+TGG | + | 24089:4556-4575 | MS.gene99342:CDS | 20.0% |
| ! | TCAATAGAAATGCCAAAATA+AGG | - | 24089:4571-4590 | MS.gene99342:intergenic | 25.0% |
| !! | AAAAATGGATTTGTATTGTC+GGG | - | 24089:5113-5132 | MS.gene99342:intergenic | 25.0% |
| !! | CAAAAATGGATTTGTATTGT+CGG | - | 24089:5114-5133 | MS.gene99342:intergenic | 25.0% |
| AGACTTCGTTGCAAATAAAT+TGG | + | 24089:4958-4977 | MS.gene99342:CDS | 30.0% | |
| CCACTTTACTTTCAAAAGAT+CGG | - | 24089:4606-4625 | MS.gene99342:intergenic | 30.0% | |
| ! | AAAATGGATTTGTATTGTCG+GGG | - | 24089:5112-5131 | MS.gene99342:intergenic | 30.0% |
| ! | CGACAATACAAATCCATTTT+TGG | + | 24089:5112-5131 | MS.gene99342:CDS | 30.0% |
| CAAAAGAAATACGATTGCCA+AGG | - | 24089:4690-4709 | MS.gene99342:intergenic | 35.0% | |
| TGCAAATAAATTGGAAGACC+CGG | + | 24089:4967-4986 | MS.gene99342:CDS | 35.0% | |
| ! | AAATGGATTTGTATTGTCGG+GGG | - | 24089:5111-5130 | MS.gene99342:intergenic | 35.0% |
| !! | CCGATCTTTTGAAAGTAAAG+TGG | + | 24089:4603-4622 | MS.gene99342:CDS | 35.0% |
| AAGAAATACGATTGCCAAGG+CGG | - | 24089:4687-4706 | MS.gene99342:intergenic | 40.0% | |
| CAAATAAATTGGAAGACCCG+GGG | + | 24089:4969-4988 | MS.gene99342:CDS | 40.0% | |
| CAGAAGGATACATCCAAAGA+GGG | - | 24089:4739-4758 | MS.gene99342:intergenic | 40.0% | |
| CTCTAAACCTCCTCTTTACT+CGG | + | 24089:4642-4661 | MS.gene99342:CDS | 40.0% | |
| CTCTTTGGATGTATCCTTCT+GGG | + | 24089:4738-4757 | MS.gene99342:CDS | 40.0% | |
| GCAAATAAATTGGAAGACCC+GGG | + | 24089:4968-4987 | MS.gene99342:CDS | 40.0% | |
| GTTTAGAGACCTGCTGAAAA+AGG | - | 24089:4630-4649 | MS.gene99342:intergenic | 40.0% | |
| TCTTTGGATGTATCCTTCTG+GGG | + | 24089:4739-4758 | MS.gene99342:CDS | 40.0% | |
| !!! | AATCCATTTTTGGCTTCTCC+CGG | + | 24089:5122-5141 | MS.gene99342:CDS | 40.0% |
| !!! | TAAAGTGGACCTTTTTCAGC+AGG | + | 24089:4618-4637 | MS.gene99342:CDS | 40.0% |
| CATCCAAAGAGGGATTTCCT+CGG | - | 24089:4729-4748 | MS.gene99342:intergenic | 45.0% | |
| CCAGAAGGATACATCCAAAG+AGG | - | 24089:4740-4759 | MS.gene99342:intergenic | 45.0% | |
| CCTCTTTGGATGTATCCTTC+TGG | + | 24089:4737-4756 | MS.gene99342:CDS | 45.0% | |
| CGTAGCGCTTGCTCATAAAT+TGG | - | 24089:5007-5026 | MS.gene99342:intergenic | 45.0% | |
| TCTCGAGTTCATACCATACC+AGG | - | 24089:5030-5049 | MS.gene99342:intergenic | 45.0% | |
| TGCTCATAAATTGGACCCTG+CGG | - | 24089:4998-5017 | MS.gene99342:intergenic | 45.0% | |
| TGGTATGAACTCGAGATCGA+TGG | + | 24089:5034-5053 | MS.gene99342:CDS | 45.0% | |
| !!! | ATCCATTTTTGGCTTCTCCC+GGG | + | 24089:5123-5142 | MS.gene99342:CDS | 45.0% |
| AACCTCCTCTTTACTCGGCT+CGG | + | 24089:4647-4666 | MS.gene99342:CDS | 50.0% | |
| AGCTGAAGGAGGGCTATGTA+TGG | - | 24089:4902-4921 | MS.gene99342:intergenic | 50.0% | |
| ATAGCCCTCCTTCAGCTAGA+GGG | + | 24089:4905-4924 | MS.gene99342:CDS | 50.0% | |
| CGCTCCTATCCAAAATGGGT+CGG | + | 24089:4829-4848 | MS.gene99342:CDS | 50.0% | |
| GAGGGCTATGTATGGTTTCG+CGG | - | 24089:4894-4913 | MS.gene99342:intergenic | 50.0% | |
| GATCGATGGCAGTACTAACC+AGG | + | 24089:5048-5067 | MS.gene99342:CDS | 50.0% | |
| TATCCAAAATGGGTCGGCGT+CGG | + | 24089:4835-4854 | MS.gene99342:CDS | 50.0% | |
| ! | CGACCCATTTTGGATAGGAG+CGG | - | 24089:4831-4850 | MS.gene99342:intergenic | 50.0% |
| !!! | TCCATTTTTGGCTTCTCCCG+GGG | + | 24089:5124-5143 | MS.gene99342:CDS | 50.0% |
| AATACGATTGCCAAGGCGGC+CGG | - | 24089:4683-4702 | MS.gene99342:intergenic | 55.0% | |
| ACCTCCTCTTTACTCGGCTC+GGG | + | 24089:4648-4667 | MS.gene99342:CDS | 55.0% | |
| CATAGCCCTCCTTCAGCTAG+AGG | + | 24089:4904-4923 | MS.gene99342:CDS | 55.0% | |
| CCGCCGCTCCTATCCAAAAT+GGG | + | 24089:4825-4844 | MS.gene99342:CDS | 55.0% | |
| GCTATGTATGGTTTCGCGGC+CGG | - | 24089:4890-4909 | MS.gene99342:intergenic | 55.0% | |
| GTGCCGAGGAAATCCCTCTT+TGG | + | 24089:4723-4742 | MS.gene99342:CDS | 55.0% | |
| TAGCCCTCCTTCAGCTAGAG+GGG | + | 24089:4906-4925 | MS.gene99342:CDS | 55.0% | |
| TTATGAGCAAGCGCTACGCC+TGG | + | 24089:5009-5028 | MS.gene99342:CDS | 55.0% | |
| ! | CCATTTTGGATAGGAGCGGC+GGG | - | 24089:4827-4846 | MS.gene99342:intergenic | 55.0% |
| ! | CCCATTTTGGATAGGAGCGG+CGG | - | 24089:4828-4847 | MS.gene99342:intergenic | 55.0% |
| ! | GACGCCGACCCATTTTGGAT+AGG | - | 24089:4836-4855 | MS.gene99342:intergenic | 55.0% |
| !! | AGCGCTGGCTGGTTTCACTA+AGG | + | 24089:5080-5099 | MS.gene99342:CDS | 55.0% |
| !! | CTCTTTACTCGGCTCGGGTT+CGG | + | 24089:4653-4672 | MS.gene99342:CDS | 55.0% |
| !! | GCGCTGGCTGGTTTCACTAA+GGG | + | 24089:5081-5100 | MS.gene99342:CDS | 55.0% |
| !! | TATCCTTCTGGGGCTGATGC+TGG | + | 24089:4749-4768 | MS.gene99342:CDS | 55.0% |
| !! | TCTTTACTCGGCTCGGGTTC+GGG | + | 24089:4654-4673 | MS.gene99342:CDS | 55.0% |
| !!! | CATTTTTGGCTTCTCCCGGG+GGG | + | 24089:5126-5145 | MS.gene99342:CDS | 55.0% |
| !!! | CCATTTTTGGCTTCTCCCGG+GGG | + | 24089:5125-5144 | MS.gene99342:CDS | 55.0% |
| ACCAGGACGAACTTCAGCGC+TGG | + | 24089:5065-5084 | MS.gene99342:CDS | 60.0% | |
| ACCCGAGCCGAGTAAAGAGG+AGG | - | 24089:4652-4671 | MS.gene99342:intergenic | 60.0% | |
| AGCAAGCGCTACGCCTGGTA+TGG | + | 24089:5014-5033 | MS.gene99342:CDS | 60.0% | |
| AGCCCTCCTTCAGCTAGAGG+GGG | + | 24089:4907-4926 | MS.gene99342:CDS | 60.0% | |
| CCCCCGGGAGAAGCCAAAAA+TGG | - | 24089:5128-5147 | MS.gene99342:intergenic | 60.0% | |
| CCCGCCGCTCCTATCCAAAA+TGG | + | 24089:4824-4843 | MS.gene99342:CDS | 60.0% | |
| CGAACCCGAGCCGAGTAAAG+AGG | - | 24089:4655-4674 | MS.gene99342:intergenic | 60.0% | |
| CTCCCCCTCTAGCTGAAGGA+GGG | - | 24089:4912-4931 | MS.gene99342:intergenic | 60.0% | |
| GCGAGCACTATTCAGTGCCG+AGG | + | 24089:4709-4728 | MS.gene99342:CDS | 60.0% | |
| GGGAAGGCTGATGCTGCTCT+TGG | - | 24089:4793-4812 | MS.gene99342:intergenic | 60.0% | |
| TCCTTCAGCTAGAGGGGGAG+CGG | + | 24089:4912-4931 | MS.gene99342:CDS | 60.0% | |
| TGGCTGCTCCACGGAACTAG+TGG | - | 24089:4864-4883 | MS.gene99342:intergenic | 60.0% | |
| ! | AAGCCGACGCCGACCCATTT+TGG | - | 24089:4841-4860 | MS.gene99342:intergenic | 60.0% |
| ! | GTCGGCTTCCACTAGTTCCG+TGG | + | 24089:4853-4872 | MS.gene99342:CDS | 60.0% |
| !! | CTTTACTCGGCTCGGGTTCG+GGG | + | 24089:4655-4674 | MS.gene99342:CDS | 60.0% |
| !! | TTTACTCGGCTCGGGTTCGG+GGG | + | 24089:4656-4675 | MS.gene99342:CDS | 60.0% |
| GAGCCAGCATCAGCCCCAGA+AGG | - | 24089:4755-4774 | MS.gene99342:intergenic | 65.0% | |
| GCTCCCCCTCTAGCTGAAGG+AGG | - | 24089:4913-4932 | MS.gene99342:intergenic | 65.0% | |
| TATGGTTTCGCGGCCGGAGC+TGG | - | 24089:4884-4903 | MS.gene99342:intergenic | 65.0% | |
| TCCGCTCCCCCTCTAGCTGA+AGG | - | 24089:4916-4935 | MS.gene99342:intergenic | 65.0% | |
| TTCCGTGGAGCAGCCAGCTC+CGG | + | 24089:4868-4887 | MS.gene99342:CDS | 65.0% | |
| ! | GCCAGCGCTGAAGTTCGTCC+TGG | - | 24089:5069-5088 | MS.gene99342:intergenic | 65.0% |
| !! | GGACGAACTTCAGCGCTGGC+TGG | + | 24089:5069-5088 | MS.gene99342:CDS | 65.0% |
| ATTGGACCCTGCGGCTGCCC+CGG | - | 24089:4989-5008 | MS.gene99342:intergenic | 70.0% | |
| CAGCATCAGCCTTCCCGCCC+CGG | + | 24089:4797-4816 | MS.gene99342:CDS | 70.0% | |
| ATAGGAGCGGCGGGTCCGCC+GGG | - | 24089:4818-4837 | MS.gene99342:intergenic | 75.0% | |
| CATCAGCCTTCCCGCCCCGG+CGG | + | 24089:4800-4819 | MS.gene99342:CDS | 75.0% | |
| GAAGACCCGGGGCAGCCGCA+GGG | + | 24089:4980-4999 | MS.gene99342:CDS | 75.0% | |
| GATAGGAGCGGCGGGTCCGC+CGG | - | 24089:4819-4838 | MS.gene99342:intergenic | 75.0% | |
| GGCCGGAGCTGGCTGCTCCA+CGG | - | 24089:4873-4892 | MS.gene99342:intergenic | 75.0% | |
| TTGGACCCTGCGGCTGCCCC+GGG | - | 24089:4988-5007 | MS.gene99342:intergenic | 75.0% | |
| !! | TCGGCTCGGGTTCGGGGGTC+CGG | + | 24089:4661-4680 | MS.gene99342:CDS | 75.0% |
| ! | GGAAGACCCGGGGCAGCCGC+AGG | + | 24089:4979-4998 | MS.gene99342:CDS | 80.0% |
| ! | GTTCGGGGGTCCGGCCGCCT+TGG | + | 24089:4670-4689 | MS.gene99342:CDS | 80.0% |
| ! | TAGGAGCGGCGGGTCCGCCG+GGG | - | 24089:4817-4836 | MS.gene99342:intergenic | 80.0% |
| ! | AGCGGCGGGTCCGCCGGGGC+GGG | - | 24089:4813-4832 | MS.gene99342:intergenic | 90.0% |
| ! | GAGCGGCGGGTCCGCCGGGG+CGG | - | 24089:4814-4833 | MS.gene99342:intergenic | 90.0% |
| ! | GCGGGTCCGCCGGGGCGGGA+AGG | - | 24089:4809-4828 | MS.gene99342:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24089 | gene | 4527 | 5159 | 4527 | ID=MS.gene99342 |
| 24089 | mRNA | 4527 | 5159 | 4527 | ID=MS.gene99342.t1;Parent=MS.gene99342 |
| 24089 | exon | 4527 | 5159 | 4527 | ID=MS.gene99342.t1.exon1;Parent=MS.gene99342.t1 |
| 24089 | CDS | 4527 | 5159 | 4527 | ID=cds.MS.gene99342.t1;Parent=MS.gene99342.t1 |
| Gene Sequence |
| Protein sequence |
