AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050273


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTTCTTCACATTCTTC+TGG 0.263869 7.3:-9307340 None:intergenic
TAATCTGGTGGAGAATCTTT+TGG 0.319672 7.3:+9307239 MS.gene050273:CDS
AAAAGCCATACTTGTTATAT+CGG 0.374988 7.3:+9307280 MS.gene050273:CDS
GCATTCCGATATAACAAGTA+TGG 0.382162 7.3:-9307285 None:intergenic
AATGCGTCACAAGAAGTTAA+TGG 0.399188 7.3:+9307303 MS.gene050273:CDS
GTTTATTATGACAGATAATC+TGG 0.406670 7.3:+9307224 MS.gene050273:intron
TTCAGTTTATTGTGATGAAT+CGG 0.436878 7.3:+9306462 MS.gene050273:CDS
GTGATGAATCGGCGAAATAC+AGG 0.457248 7.3:+9306473 MS.gene050273:CDS
GTCACAAGAAGTTAATGGTA+TGG 0.533977 7.3:+9307308 MS.gene050273:CDS
TTCTTCAGGAATGCAAACCT+CGG 0.576586 7.3:+9306439 None:intergenic
TATTATGACAGATAATCTGG+TGG 0.659213 7.3:+9307227 MS.gene050273:intron
CATCACAATAAACTGAACCG+AGG 0.694555 7.3:-9306456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACACTATAATTTCAACAA+CGG - chr7.3:9306521-9306540 None:intergenic 20.0%
!!! TGTTTTTCAGATTTGTTATA+AGG + chr7.3:9306536-9306555 MS.gene050273:intron 20.0%
! ACATAGAAAGTAATCAAGTA+GGG - chr7.3:9307201-9307220 None:intergenic 25.0%
! CAACAATATACAAGTTCTTT+AGG - chr7.3:9306614-9306633 None:intergenic 25.0%
! GTTTATTATGACAGATAATC+TGG + chr7.3:9307224-9307243 MS.gene050273:intron 25.0%
! TTCAGTTTATTGTGATGAAT+CGG + chr7.3:9306462-9306481 MS.gene050273:CDS 25.0%
!! GATTGATAATTGAATGAAAG+TGG + chr7.3:9306694-9306713 MS.gene050273:intron 25.0%
!!! AAAAGCCATACTTGTTATAT+CGG + chr7.3:9307280-9307299 MS.gene050273:CDS 25.0%
!!! ACAATGATTATTTTACTGTG+TGG + chr7.3:9306825-9306844 MS.gene050273:intron 25.0%
!!! TTCAGATTTGTTATAAGGAT+CGG + chr7.3:9306541-9306560 MS.gene050273:intron 25.0%
CACATAGAAAGTAATCAAGT+AGG - chr7.3:9307202-9307221 None:intergenic 30.0%
CATAGAAAGTAATCAAGTAG+GGG - chr7.3:9307200-9307219 None:intergenic 30.0%
GTTATTTAATACAGCTGTAG+TGG + chr7.3:9307076-9307095 MS.gene050273:intron 30.0%
TATTATGACAGATAATCTGG+TGG + chr7.3:9307227-9307246 MS.gene050273:intron 30.0%
! AGGTTTGTTCTAATTCAGTA+AGG - chr7.3:9307119-9307138 None:intergenic 30.0%
! TGATCCACAATTTAAAACAC+TGG + chr7.3:9307147-9307166 MS.gene050273:intron 30.0%
! TGTTTTATAGCTAGCTATAG+TGG + chr7.3:9306932-9306951 MS.gene050273:intron 30.0%
!! GTGTTTTAAATTGTGGATCA+TGG - chr7.3:9307147-9307166 None:intergenic 30.0%
!! TACACCAGTGTTTTAAATTG+TGG - chr7.3:9307154-9307173 None:intergenic 30.0%
AAATAACCGCTATAGTGTAG+CGG + chr7.3:9306860-9306879 MS.gene050273:intron 35.0%
AATGCGTCACAAGAAGTTAA+TGG + chr7.3:9307303-9307322 MS.gene050273:CDS 35.0%
AATTGTGGATCATGGAAAAG+AGG - chr7.3:9307139-9307158 None:intergenic 35.0%
ACATCACACAACAACACAAT+TGG - chr7.3:9306764-9306783 None:intergenic 35.0%
ATAGTGCATTGCAGAACAAA+AGG - chr7.3:9306898-9306917 None:intergenic 35.0%
CAACCAAATTGCAGAATAGA+TGG - chr7.3:9307025-9307044 None:intergenic 35.0%
GCATTCCGATATAACAAGTA+TGG - chr7.3:9307288-9307307 None:intergenic 35.0%
GTCACAAGAAGTTAATGGTA+TGG + chr7.3:9307308-9307327 MS.gene050273:CDS 35.0%
GTTCTAATTCAGTAAGGCTA+CGG - chr7.3:9307113-9307132 None:intergenic 35.0%
TAAATTCCGCTACACTATAG+CGG - chr7.3:9306869-9306888 None:intergenic 35.0%
TAATCAAGTAGGGGAATAGA+TGG - chr7.3:9307191-9307210 None:intergenic 35.0%
TTGTTCTGCAATGCACTATT+TGG + chr7.3:9306899-9306918 MS.gene050273:intron 35.0%
! TAATCTGGTGGAGAATCTTT+TGG + chr7.3:9307239-9307258 MS.gene050273:CDS 35.0%
CAGAATAGATGGCACAGATT+TGG - chr7.3:9307014-9307033 None:intergenic 40.0%
CATCACAATAAACTGAACCG+AGG - chr7.3:9306459-9306478 None:intergenic 40.0%
GTGCCATCTATTCTGCAATT+TGG + chr7.3:9307019-9307038 MS.gene050273:intron 40.0%
TTCACCAATGTTGTCAATCG+CGG - chr7.3:9306988-9307007 None:intergenic 40.0%
AAGTAGGGGAATAGATGGCA+CGG - chr7.3:9307186-9307205 None:intergenic 45.0%
CGGATCATTCCACCAAGATA+CGG - chr7.3:9306968-9306987 None:intergenic 45.0%
GTGATGAATCGGCGAAATAC+AGG + chr7.3:9306473-9306492 MS.gene050273:CDS 45.0%
TCGCGCTAAATAGTGCATTG+AGG - chr7.3:9307053-9307072 None:intergenic 45.0%
TGATCCGCGATTGACAACAT+TGG + chr7.3:9306981-9307000 MS.gene050273:intron 45.0%
TTAATACAGCTGTAGTGGCG+CGG + chr7.3:9307081-9307100 MS.gene050273:intron 45.0%
!! GCGAAAGCACCGTATCTTGG+TGG + chr7.3:9306956-9306975 MS.gene050273:intron 55.0%
!! GGCGCGAAAGCACCGTATCT+TGG + chr7.3:9306953-9306972 MS.gene050273:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 9306447 9307349 9306447 ID=MS.gene050273
chr7.3 mRNA 9306447 9307349 9306447 ID=MS.gene050273.t1;Parent=MS.gene050273
chr7.3 exon 9306447 9306494 9306447 ID=MS.gene050273.t1.exon1;Parent=MS.gene050273.t1
chr7.3 CDS 9306447 9306494 9306447 ID=cds.MS.gene050273.t1;Parent=MS.gene050273.t1
chr7.3 exon 9307238 9307349 9307238 ID=MS.gene050273.t1.exon2;Parent=MS.gene050273.t1
chr7.3 CDS 9307238 9307349 9307238 ID=cds.MS.gene050273.t1;Parent=MS.gene050273.t1
Gene Sequence

>MS.gene050273

GAATGCAAACCTCGGTTCAGTTTATTGTGATGAATCGGCGAAATACAGGTTCAGATTTGTGTTTGTTAATTCCGTTGTTGAAATTATAGTGTTTTTCAGATTTGTTATAAGGATCGGTTATAGTTTGTATTAGATCATTCGTTTCTGATTGCATTCAATTGAAACCTAAAGAACTTGTATATTGTTGATTACTGAATGCTACTTAATTGTTGAATTAATTCTGAATGCTAATTACTATTCATTCTATGATTGATAATTGAATGAAAGTGGATCGAGTTTTTTTTTTTTTTTTTTTGAAAAAGTATTACATCAAACCAATTGTGTTGTTGTGTGATGTTATTATCTGCAGATGTAGATTTATTCTAAATAATTGTTGTTACAATGATTATTTTACTGTGTGGAGCAGTGTTGTCAAATAACCGCTATAGTGTAGCGGAATTTAAAATCGCCTTTTGTTCTGCAATGCACTATTTGGCGCGAAATGTTGTTTTATAGCTAGCTATAGTGGCGCGAAAGCACCGTATCTTGGTGGAATGATCCGCGATTGACAACATTGGTGAATGTCCAAATCTGTGCCATCTATTCTGCAATTTGGTTGTAGTTCCTCAATGCACTATTTAGCGCGAAGTGTTATTTAATACAGCTGTAGTGGCGCGGAAAGCACCGTAGCCTTACTGAATTAGAACAAACCTCTTTTCCATGATCCACAATTTAAAACACTGGTGTATGTATGAATCCGTGCCATCTATTCCCCTACTTGATTACTTTCTATGTGATGTTTATTATGACAGATAATCTGGTGGAGAATCTTTTGGATTTTGAGTATGAACTGAAAAAGCCATACTTGTTATATCGGAATGCGTCACAAGAAGTTAATGGTATGGTTTTACAATCCAGAAGAAT

Protein sequence

>MS.gene050273.t1

ECKPRFSLLUUIGEIQIIWWRIFWILSMNUKSHTCYIGMRHKKLMVWFYNPEE