AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99550.t1 MTR_8g045735 94.624 93 5 0 1 93 62 154 3.74e-61 183
MS.gene99550.t1 MTR_8g045695 88.421 95 11 0 1 95 62 156 3.74e-59 178
MS.gene99550.t1 MTR_8g045665 86.316 95 13 0 1 95 62 156 1.33e-56 172
MS.gene99550.t1 MTR_8g045560 84.211 95 15 0 1 95 62 156 8.62e-55 167
MS.gene99550.t1 MTR_8g045640 84.211 95 15 0 1 95 62 156 1.68e-54 166
MS.gene99550.t1 MTR_8g045520 68.750 96 29 1 1 95 62 157 3.54e-44 140
MS.gene99550.t1 MTR_8g045570 70.330 91 26 1 1 90 62 152 5.94e-44 139
MS.gene99550.t1 MTR_8g045555 67.033 91 29 1 1 90 62 152 2.32e-42 135
MS.gene99550.t1 MTR_8g045490 65.625 96 31 2 1 95 62 156 3.60e-41 132
MS.gene99550.t1 MTR_8g045300 63.830 94 33 1 2 94 63 156 1.55e-40 131
MS.gene99550.t1 MTR_8g045400 63.542 96 33 2 1 95 62 156 1.70e-40 131
MS.gene99550.t1 MTR_1g030810 40.217 92 52 2 1 89 61 152 3.16e-19 77.0
MS.gene99550.t1 MTR_1g030820 38.043 92 54 2 1 89 61 152 1.44e-17 72.8
MS.gene99550.t1 MTR_8g012550 39.560 91 53 2 3 92 66 155 2.40e-17 72.4
MS.gene99550.t1 MTR_1g031640 41.758 91 46 4 1 90 63 147 4.21e-12 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99550.t1 AT1G70870 45.556 90 47 2 1 89 50 138 4.61e-19 76.3
MS.gene99550.t1 AT5G28010 35.870 92 57 2 2 91 75 166 2.84e-14 64.7
MS.gene99550.t1 AT5G28010 35.870 92 57 2 2 91 75 166 2.84e-14 64.7
MS.gene99550.t1 AT1G70850 38.043 92 55 2 2 91 225 316 2.05e-13 64.3
MS.gene99550.t1 AT1G70850 38.043 92 55 2 2 91 225 316 2.05e-13 64.3
MS.gene99550.t1 AT5G28000 36.264 91 56 2 3 91 74 164 6.83e-13 60.8
MS.gene99550.t1 AT1G70840 34.783 92 58 2 2 91 80 171 1.18e-12 60.5
MS.gene99550.t1 AT1G23120 33.708 89 57 2 1 88 58 145 1.23e-12 60.1
MS.gene99550.t1 AT1G23120 33.708 89 57 2 1 88 108 195 2.48e-12 60.1
MS.gene99550.t1 AT1G24020 36.047 86 53 2 5 89 67 151 3.23e-12 58.9
MS.gene99550.t1 AT1G24020 36.047 86 53 2 5 89 67 151 3.23e-12 58.9
MS.gene99550.t1 AT1G70890 35.870 92 57 2 2 91 67 158 4.21e-12 58.9

Find 22 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATAACTTTCAAGTTGTT+TGG 0.327904 8.1:+59637187 MS.gene99550:CDS
AAGATGACCATCAATATCTT+TGG 0.335693 8.1:-59637365 None:intergenic
ATGGAAGTTCTGCTGTTAGA+TGG 0.411879 8.1:+59637269 MS.gene99550:CDS
TGGAGATATTGAAAACTATA+AGG 0.439418 8.1:+59637210 MS.gene99550:CDS
GAAGTGCTTAACAAGGCTAA+TGG 0.442453 8.1:+59637250 MS.gene99550:CDS
AGGTAGTCCATCCAGCCATT+TGG 0.456227 8.1:-59637331 None:intergenic
AAAGGAACCAAAGATATTGA+TGG 0.467555 8.1:+59637358 MS.gene99550:CDS
ATAACTTTCAAGTTGTTTGG+TGG 0.479625 8.1:+59637190 MS.gene99550:CDS
TATCTTTGGTTCCTTTGCTA+AGG 0.518303 8.1:-59637351 None:intergenic
TAACATAACAACAGATGGTA+AGG 0.521132 8.1:+59637117 MS.gene99550:intron
ATGTTGATACTCCAAATGGC+TGG 0.578150 8.1:+59637320 MS.gene99550:CDS
ATTGATGGTCATCTTGTCAA+GGG 0.586933 8.1:+59637373 MS.gene99550:CDS
TGATACTCCAAATGGCTGGA+TGG 0.587093 8.1:+59637324 MS.gene99550:CDS
TATTGATGGTCATCTTGTCA+AGG 0.589389 8.1:+59637372 MS.gene99550:CDS
GAGGATGTTGATACTCCAAA+TGG 0.593330 8.1:+59637316 MS.gene99550:CDS
AGGTACACACATGCAATGAG+AGG 0.626711 8.1:+59637137 MS.gene99550:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.631478 8.1:+59637243 MS.gene99550:CDS
TTGATGGTCATCTTGTCAAG+GGG 0.646397 8.1:+59637374 MS.gene99550:CDS
TGGATGGACTACCTTAGCAA+AGG 0.650698 8.1:+59637340 MS.gene99550:CDS
GGTACACACATGCAATGAGA+GGG 0.691358 8.1:+59637138 MS.gene99550:CDS
TGATTATGAGAAGAACAATG+AGG 0.694185 8.1:+59637297 MS.gene99550:CDS
TGATGGTCATCTTGTCAAGG+GGG 0.696400 8.1:+59637375 MS.gene99550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CGTAAAATTAAATAAAAAAA+GGG + chr8.1:59635987-59636006 MS.gene99550:intron 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG + chr8.1:59637081-59637100 MS.gene99550:intron 10.0%
!! GCGTAAAATTAAATAAAAAA+AGG + chr8.1:59635986-59636005 MS.gene99550:intron 15.0%
!! TTAGAACTAAAATAAAATTG+CGG + chr8.1:59636350-59636369 MS.gene99550:intron 15.0%
!!! AAGAAAAATTATACTTTTCA+AGG + chr8.1:59635740-59635759 MS.gene99550:intron 15.0%
!!! TAAATTTTCACTTTTTGTTT+TGG - chr8.1:59636609-59636628 None:intergenic 15.0%
!!! TAAGATAAAGTTTTTACTTT+TGG + chr8.1:59636996-59637015 MS.gene99550:intron 15.0%
!!! TATTAAAGTCAAACTTTTAT+CGG + chr8.1:59636734-59636753 MS.gene99550:intron 15.0%
!! ATCAAAAATCAAATATCACA+AGG - chr8.1:59636705-59636724 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG - chr8.1:59636832-59636851 None:intergenic 20.0%
!! ATTTATCTTTCTAGATCTAA+TGG + chr8.1:59636123-59636142 MS.gene99550:intron 20.0%
!! TATTACATACAACTCATATA+GGG - chr8.1:59636067-59636086 None:intergenic 20.0%
!!! ATTATTGTATTTTCTCTCTT+TGG - chr8.1:59636498-59636517 None:intergenic 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG + chr8.1:59636857-59636876 MS.gene99550:intron 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG + chr8.1:59636837-59636856 MS.gene99550:intron 20.0%
!!! TTATTGTATTTTCTCTCTTT+GGG - chr8.1:59636497-59636516 None:intergenic 20.0%
! AATGCTAACAAATGTTCTTA+AGG - chr8.1:59637036-59637055 None:intergenic 25.0%
! ATAGAGGGAAATTATTGTTA+AGG + chr8.1:59636377-59636396 MS.gene99550:intron 25.0%
! ATTACATACAACTCATATAG+GGG - chr8.1:59636066-59636085 None:intergenic 25.0%
! ATTGCCTTTATAAGTTCTTT+AGG - chr8.1:59635778-59635797 None:intergenic 25.0%
! CAAATCAAATTGTGTTCAAA+TGG - chr8.1:59636550-59636569 None:intergenic 25.0%
! CTATTACATACAACTCATAT+AGG - chr8.1:59636068-59636087 None:intergenic 25.0%
! GTGCTAAATTATTTATGCTT+AGG + chr8.1:59636031-59636050 MS.gene99550:intron 25.0%
! TGGAGATATTGAAAACTATA+AGG + chr8.1:59637210-59637229 MS.gene99550:CDS 25.0%
! TTCTTAGACAAATTGTAGTA+CGG - chr8.1:59635725-59635744 None:intergenic 25.0%
!!! AAGATAACTTTCAAGTTGTT+TGG + chr8.1:59637187-59637206 MS.gene99550:CDS 25.0%
!!! AATTTTTCGATCTAGTGTAA+TGG + chr8.1:59636231-59636250 MS.gene99550:intron 25.0%
!!! ATTTTACTAATGAAGAGTAC+AGG + chr8.1:59636458-59636477 MS.gene99550:intron 25.0%
!!! ATTTTCACTTTTTGTTTTGG+TGG - chr8.1:59636606-59636625 None:intergenic 25.0%
!!! CTGTTTAGGTTATTTTGATT+AGG - chr8.1:59636580-59636599 None:intergenic 25.0%
!!! GTAATGGATAAGTAATTACA+TGG + chr8.1:59636247-59636266 MS.gene99550:intron 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG + chr8.1:59636710-59636729 MS.gene99550:intron 25.0%
AAAGGAACCAAAGATATTGA+TGG + chr8.1:59637358-59637377 MS.gene99550:CDS 30.0%
AAGATGACCATCAATATCTT+TGG - chr8.1:59637368-59637387 None:intergenic 30.0%
AATCAAACAAGATGATGACT+TGG - chr8.1:59635636-59635655 None:intergenic 30.0%
ACAATATCTCTTGGAAGTTA+CGG - chr8.1:59636657-59636676 None:intergenic 30.0%
ACCATCTGTTGTTATGTTAA+AGG - chr8.1:59637116-59637135 None:intergenic 30.0%
ATAAAATTGCGGAATGATAG+AGG + chr8.1:59636361-59636380 MS.gene99550:intron 30.0%
CTTAGGGTTAATAAAACGTA+TGG + chr8.1:59636415-59636434 MS.gene99550:intron 30.0%
GCTACAAAGTTAATCTCTTA+GGG + chr8.1:59636399-59636418 MS.gene99550:intron 30.0%
TAAAATTGCGGAATGATAGA+GGG + chr8.1:59636362-59636381 MS.gene99550:intron 30.0%
TAACATAACAACAGATGGTA+AGG + chr8.1:59637117-59637136 MS.gene99550:intron 30.0%
TAATTACATGGCACATATCT+AGG + chr8.1:59636259-59636278 MS.gene99550:intron 30.0%
TGATTATGAGAAGAACAATG+AGG + chr8.1:59637297-59637316 MS.gene99550:CDS 30.0%
TGGACCTAAAGAACTTATAA+AGG + chr8.1:59635771-59635790 MS.gene99550:intron 30.0%
! AGTATGTCTGAAGTGAATAT+AGG - chr8.1:59635854-59635873 None:intergenic 30.0%
! GTTTGACTTTAATAACCACA+AGG - chr8.1:59636728-59636747 None:intergenic 30.0%
! TGATTTTAAACTATCCTGTG+AGG + chr8.1:59636311-59636330 MS.gene99550:intron 30.0%
!! ATAACTTTCAAGTTGTTTGG+TGG + chr8.1:59637190-59637209 MS.gene99550:CDS 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG + chr8.1:59636074-59636093 MS.gene99550:intron 30.0%
!! TACTTTTCAAGGCAATAATC+TGG + chr8.1:59635751-59635770 MS.gene99550:intron 30.0%
AATCCTTAACTAGTGCTCTA+AGG - chr8.1:59636899-59636918 None:intergenic 35.0%
ATCCTTAACTAGTGCTCTAA+GGG - chr8.1:59636898-59636917 None:intergenic 35.0%
CCAAGAGATATTGTGCTTTA+TGG + chr8.1:59636663-59636682 MS.gene99550:intron 35.0%
CCATAAAGCACAATATCTCT+TGG - chr8.1:59636666-59636685 None:intergenic 35.0%
GATTAACTTTGTAACCTCAC+AGG - chr8.1:59636328-59636347 None:intergenic 35.0%
GCCTTTAACATAACAACAGA+TGG + chr8.1:59637112-59637131 MS.gene99550:intron 35.0%
GGCTACAAAGTTAATCTCTT+AGG + chr8.1:59636398-59636417 MS.gene99550:intron 35.0%
TGGCACATATCTAGGTAAAA+CGG + chr8.1:59636267-59636286 MS.gene99550:intron 35.0%
TGTGTTCAAATGGAACTTTG+TGG - chr8.1:59636540-59636559 None:intergenic 35.0%
! AATTCAAGTTCGAGTTCAAC+TGG + chr8.1:59636182-59636201 MS.gene99550:intron 35.0%
! ATTGATGGTCATCTTGTCAA+GGG + chr8.1:59637373-59637392 MS.gene99550:CDS 35.0%
! GCATCGATATCCTTATCATT+AGG + chr8.1:59636819-59636838 MS.gene99550:intron 35.0%
! GTTTGCTACGATAGCAAAAA+GGG - chr8.1:59636635-59636654 None:intergenic 35.0%
! TATCTTTGGTTCCTTTGCTA+AGG - chr8.1:59637354-59637373 None:intergenic 35.0%
! TATTGATGGTCATCTTGTCA+AGG + chr8.1:59637372-59637391 MS.gene99550:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG + chr8.1:59637243-59637262 MS.gene99550:CDS 35.0%
ATGGAACTTTGTGGTCGTAA+TGG - chr8.1:59636531-59636550 None:intergenic 40.0%
ATGGAAGTTCTGCTGTTAGA+TGG + chr8.1:59637269-59637288 MS.gene99550:CDS 40.0%
ATGTTGATACTCCAAATGGC+TGG + chr8.1:59637320-59637339 MS.gene99550:CDS 40.0%
CTATACCAAGCAACTCATGT+GGG + chr8.1:59636158-59636177 MS.gene99550:intron 40.0%
GAGGATGTTGATACTCCAAA+TGG + chr8.1:59637316-59637335 MS.gene99550:CDS 40.0%
TATACCAAGCAACTCATGTG+GGG + chr8.1:59636159-59636178 MS.gene99550:intron 40.0%
! GAAGTGCTTAACAAGGCTAA+TGG + chr8.1:59637250-59637269 MS.gene99550:CDS 40.0%
! TTCCCTTAGAGCACTAGTTA+AGG + chr8.1:59636893-59636912 MS.gene99550:intron 40.0%
! TTGATGGTCATCTTGTCAAG+GGG + chr8.1:59637374-59637393 MS.gene99550:CDS 40.0%
!! GGTTTGCTACGATAGCAAAA+AGG - chr8.1:59636636-59636655 None:intergenic 40.0%
!!! TGTTTTGGTGGCAACTGTTT+AGG - chr8.1:59636594-59636613 None:intergenic 40.0%
AATTCCCCACATGAGTTGCT+TGG - chr8.1:59636166-59636185 None:intergenic 45.0%
AGGTACACACATGCAATGAG+AGG + chr8.1:59637137-59637156 MS.gene99550:CDS 45.0%
AGTTCAACTGGATGGTTGAG+GGG + chr8.1:59636194-59636213 MS.gene99550:intron 45.0%
CATGAGTTGCTTGGTATAGC+AGG - chr8.1:59636157-59636176 None:intergenic 45.0%
GAGTTCAACTGGATGGTTGA+GGG + chr8.1:59636193-59636212 MS.gene99550:intron 45.0%
GCTATACCAAGCAACTCATG+TGG + chr8.1:59636157-59636176 MS.gene99550:intron 45.0%
GGTACACACATGCAATGAGA+GGG + chr8.1:59637138-59637157 MS.gene99550:CDS 45.0%
TCATCTTGTCAAGGGGGAAA+AGG + chr8.1:59637381-59637400 MS.gene99550:CDS 45.0%
TGATACTCCAAATGGCTGGA+TGG + chr8.1:59637324-59637343 MS.gene99550:CDS 45.0%
TGATGGTCATCTTGTCAAGG+GGG + chr8.1:59637375-59637394 MS.gene99550:CDS 45.0%
TTGCTTGGTATAGCAGGTGT+GGG - chr8.1:59636151-59636170 None:intergenic 45.0%
! CAAGTTCGAGTTCAACTGGA+TGG + chr8.1:59636186-59636205 MS.gene99550:intron 45.0%
!! TGGATGGACTACCTTAGCAA+AGG + chr8.1:59637340-59637359 MS.gene99550:CDS 45.0%
!! TTAAAGTAATATTAAAAATA+AGG - chr8.1:59637060-59637079 None:intergenic 5.0%
!!! TTTTATTTATTAATGTATTA+TGG - chr8.1:59635923-59635942 None:intergenic 5.0%
AGGTAGTCCATCCAGCCATT+TGG - chr8.1:59637334-59637353 None:intergenic 50.0%
CGAGTTCAACTGGATGGTTG+AGG + chr8.1:59636192-59636211 MS.gene99550:intron 50.0%
GTTGCTTGGTATAGCAGGTG+TGG - chr8.1:59636152-59636171 None:intergenic 50.0%
! GTTGAGGGGTAGTCTTGACT+TGG + chr8.1:59636208-59636227 MS.gene99550:intron 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 59635597 59637414 59635597 ID=MS.gene99550
chr8.1 mRNA 59635597 59637414 59635597 ID=MS.gene99550.t1;Parent=MS.gene99550
chr8.1 exon 59635597 59635600 59635597 ID=MS.gene99550.t1.exon1;Parent=MS.gene99550.t1
chr8.1 CDS 59635597 59635600 59635597 ID=cds.MS.gene99550.t1;Parent=MS.gene99550.t1
chr8.1 exon 59637131 59637414 59637131 ID=MS.gene99550.t1.exon2;Parent=MS.gene99550.t1
chr8.1 CDS 59637131 59637414 59637131 ID=cds.MS.gene99550.t1;Parent=MS.gene99550.t1
Gene Sequence

>MS.gene99550

ATGGGTAATTATGTTTCTAATTCAATTGATTCATAACCAAGTCATCATCTTGTTTGATTTGAAAAATATTTTTTATTTCTAGAATTTTTTTTTTTAAGATTTGATACTATCATAATACATAACTACCGTACTACAATTTGTCTAAGAAAAATTATACTTTTCAAGGCAATAATCTGGACCTAAAGAACTTATAAAGGCAATAATTTACGTAATCAAAGAAATTTATATCTAAAAAATTAAAATACAAATGTGCACCTATATTCACTTCAGACATACTACTAACTAGATCGATGCACTAAATTTATCTTTATTCATTTACTACACCATAATACATTAATAAATAAAATAACAAATTACAAAAGATATAAGAATGTTGAATATTTGAAAATGCGTAAAATTAAATAAAAAAAGGGAAGAAATTATTATTAATAACAGTGCTAAATTATTTATGCTTAGGAGTATTGATCCCCTATATGAGTTGTATGTAATAGTTCTAGTGGAAATTATTTAGATTTTGAGAGATAAAATTTATCTTTCTAGATCTAATGGTTCCCACACCTGCTATACCAAGCAACTCATGTGGGGAATTCAAGTTCGAGTTCAACTGGATGGTTGAGGGGTAGTCTTGACTTGGAATTTTTCGATCTAGTGTAATGGATAAGTAATTACATGGCACATATCTAGGTAAAACGGTTATTTATAACTTTCAGATGTTGATTTTAAACTATCCTGTGAGGTTACAAAGTTAATCTCTTAGAACTAAAATAAAATTGCGGAATGATAGAGGGAAATTATTGTTAAGGCTACAAAGTTAATCTCTTAGGGTTAATAAAACGTATGGTCATTTTCTTATCAGCAACAATTTTACTAATGAAGAGTACAGGACACATACTGAATCCCAAAGAGAGAAAATACAATAATTATATGCAATCCATTACGACCACAAAGTTCCATTTGAACACAATTTGATTTGATTTGTTCCTAATCAAAATAACCTAAACAGTTGCCACCAAAACAAAAAGTGAAAATTTATTTCCCTTTTTGCTATCGTAGCAAACCGTAACTTCCAAGAGATATTGTGCTTTATGGTTATTTTATAATTGTGCCTTGTGATATTTGATTTTTGATCCTTGTGGTTATTAAAGTCAAACTTTTATCGGTAAGCTCAGAACAAAAACATGCGTTAAATGAAAACAATAAAATATTTTATTTTAGAAATAATGCATCGATATCCTTATCATTAGGTTTTTATGATTTCTTTGGTGAAACTAATATTTTTAAGGTTAGACTAACAAGTTCCCTTAGAGCACTAGTTAAGGATTAAAAAAAGAAATAATTTTTAAATTTTATATAGAAAAAATTACTTTTTGATATTTCAATAAGTCAAACACACAATTTATAAGATAAAGTTTTTACTTTTGGTTTCTTAACAAATGCCTTAAGAACATTTGTTAGCATTTCCTTATTTTTAATATTACTTTAACAAAATGTTTTTTATTGTTTTTGGTATTAATTGCCTTTAACATAACAACAGATGGTAAGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAAAACAAAAAGATAACTTTCAAGTTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTAATGGAAGTTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATGTTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAAGGAACCAAAGATATTGATGGTCATCTTGTCAAGGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MS.gene99550.t1

MDGKVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKANGSSAVRWTIDYEKNNEDVDTPNGWMDYLSKGTKDIDGHLVKGEKVAL