AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048382.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048382.01.T01 MTR_1g101400 89.773 176 14 1 96 271 28 199 3.09e-108 315
MsG0080048382.01.T01 MTR_4g129790 90.909 44 4 0 72 115 143 186 3.16e-18 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048382.01.T01 AT5G52100 75.926 162 39 0 109 270 32 193 1.38e-85 258

Find 65 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGACCTTACTATGGTTT+TGG 0.191058 contig294end:-16320 MsG0080048382.01.T01:CDS
GTGGAACAGGTTGTAATAAA+TGG 0.207909 contig294end:-16620 MsG0080048382.01.T01:intron
CACGATTTGGATTTGAGTTT+GGG 0.272951 contig294end:-17831 MsG0080048382.01.T01:CDS
AGAGATAAAGTATGTTATAT+TGG 0.274675 contig294end:-17323 MsG0080048382.01.T01:CDS
TCACGATTTGGATTTGAGTT+TGG 0.276796 contig294end:-17832 MsG0080048382.01.T01:CDS
GTTTGGGAAATTCAAGTTCT+AGG 0.285239 contig294end:-17815 MsG0080048382.01.T01:CDS
GCCAGAGGAATGGAAGTTTC+TGG 0.319689 contig294end:-16551 MsG0080048382.01.T01:CDS
CAATTTCAACGTTGCGGTAA+TGG 0.334497 contig294end:+15423 MsG0080048382.01.T01:intergenic
GACTACAACCGTTGTGGCCT+TGG 0.349381 contig294end:+16178 MsG0080048382.01.T01:intergenic
TATACTAAATGACCTTACTA+TGG 0.353599 contig294end:-16326 MsG0080048382.01.T01:CDS
CAACATGTTGGGGAAGATAT+TGG 0.357960 contig294end:-16515 MsG0080048382.01.T01:CDS
ACTATATAGGCAACATCATT+TGG 0.368467 contig294end:-15796 MsG0080048382.01.T01:intron
ATTTCACATTACCTGAGATA+TGG 0.385396 contig294end:+16294 MsG0080048382.01.T01:intergenic
GAGATAAAGTATGTTATATT+GGG 0.394913 contig294end:-17322 MsG0080048382.01.T01:CDS
GTACATGCCGCGGATCAAAT+TGG 0.402326 contig294end:-15758 MsG0080048382.01.T01:CDS
CTATTGATACTCAACATGTT+GGG 0.408524 contig294end:-16526 MsG0080048382.01.T01:CDS
GAGATGAGAAGAAGAGGAAA+CGG 0.410173 contig294end:-18884 MsG0080048382.01.T01:CDS
CTGTAATAATATTGATCCTA+TGG 0.411160 contig294end:+15469 MsG0080048382.01.T01:intergenic
TAATGCTGCAGATCACGATT+TGG 0.415240 contig294end:-17844 MsG0080048382.01.T01:CDS
TCACCCCTTGCTCACCATGC+TGG 0.433731 contig294end:+15699 MsG0080048382.01.T01:intergenic
AGTATATGACAATGTAAAAC+AGG 0.435138 contig294end:-16135 MsG0080048382.01.T01:intron
TCTTACATTTGCATTAGCCT+TGG 0.452314 contig294end:+15394 MsG0080048382.01.T01:intergenic
TTTGGGAAATTCAAGTTCTA+GGG 0.471258 contig294end:-17814 MsG0080048382.01.T01:CDS
TCCAGAAACTTCCATTCCTC+TGG 0.475788 contig294end:+16550 MsG0080048382.01.T01:intergenic
GATGGAAAGAAGAAGGAAGA+AGG 0.490833 contig294end:-18439 MsG0080048382.01.T01:CDS
TTTCCTGTAGCTTTGTGGCA+TGG 0.496064 contig294end:-16368 MsG0080048382.01.T01:intron
GCTATTGATACTCAACATGT+TGG 0.498878 contig294end:-16527 MsG0080048382.01.T01:CDS
ATGGAAAGAAGAAGGAAGAA+GGG 0.501486 contig294end:-18438 MsG0080048382.01.T01:CDS
TCGAGGGAGATGAGAAGAAG+AGG 0.505017 contig294end:-18890 None:intergenic
GTTGCTCCAACGCTTTCCAT+AGG 0.508224 contig294end:-15485 MsG0080048382.01.T01:CDS
TGATGCAGATGGAAAGAAGA+AGG 0.510322 contig294end:-18446 MsG0080048382.01.T01:CDS
AGGATCAATATTATTACAGC+AGG 0.513741 contig294end:-15465 MsG0080048382.01.T01:CDS
TTACCGCAACGTTGAAATTG+TGG 0.514733 contig294end:-15420 MsG0080048382.01.T01:CDS
TGGAGCCCAAAACCATAGTA+AGG 0.517913 contig294end:+16314 MsG0080048382.01.T01:intergenic
ACTATAACGTCAGGGTGTCT+TGG 0.518817 contig294end:-15507 MsG0080048382.01.T01:intron
GGGAATGCATTGTTCAAAGC+TGG 0.521453 contig294end:-18418 MsG0080048382.01.T01:intron
ATAGTAAGGTCATTTAGTAT+AGG 0.525371 contig294end:+16328 MsG0080048382.01.T01:intergenic
AGATGAGAAGAAGAGGAAAC+GGG 0.525399 contig294end:-18883 MsG0080048382.01.T01:intron
ACATTGTCATATACTGCAGA+AGG 0.525891 contig294end:+16143 MsG0080048382.01.T01:intergenic
AAAGAAGATTGATGCAGATG+AGG 0.536126 contig294end:-18476 MsG0080048382.01.T01:CDS
TATTAAATGACAGAATCATC+AGG 0.536179 contig294end:-17867 MsG0080048382.01.T01:intron
CGTTGAAATTGTGGAATCCA+AGG 0.538239 contig294end:-15411 MsG0080048382.01.T01:CDS
GAGGAAAGAATTGATGCAGA+TGG 0.541097 contig294end:-18457 MsG0080048382.01.T01:CDS
TGTAATAAATGGAGCAGCAA+AGG 0.543811 contig294end:-16609 MsG0080048382.01.T01:CDS
AGCAGTATTAGCTGTGACAA+AGG 0.549949 contig294end:-16573 MsG0080048382.01.T01:CDS
TGTTGGGGAAGATATTGGAC+AGG 0.558324 contig294end:-16510 MsG0080048382.01.T01:intron
ATTGATCCTATGGAAAGCGT+TGG 0.571844 contig294end:+15479 MsG0080048382.01.T01:intergenic
GCCGCGGATCAAATTGGATA+CGG 0.582539 contig294end:-15752 MsG0080048382.01.T01:CDS
TGTGACAAAGGCCAGAGGAA+TGG 0.593244 contig294end:-16561 MsG0080048382.01.T01:CDS
AATGGAGCAGCAAAGGAGGT+AGG 0.602441 contig294end:-16602 MsG0080048382.01.T01:CDS
TATTGATACTCAACATGTTG+GGG 0.603145 contig294end:-16525 MsG0080048382.01.T01:CDS
TTTAGTATAGGTATTTCAAG+AGG 0.603621 contig294end:+16340 MsG0080048382.01.T01:intergenic
ATTCCACAATTTCAACGTTG+CGG 0.605771 contig294end:+15417 MsG0080048382.01.T01:intergenic
GAATCCTCTGAATTATACTC+AGG 0.606536 contig294end:-17227 MsG0080048382.01.T01:intron
AATAAATGGAGCAGCAAAGG+AGG 0.611168 contig294end:-16606 MsG0080048382.01.T01:CDS
ATTGTCTGCATTCTGCGAGA+AGG 0.616513 contig294end:-15722 MsG0080048382.01.T01:CDS
ACCGTATCCAATTTGATCCG+CGG 0.631428 contig294end:+15751 MsG0080048382.01.T01:intergenic
CATTGTCATATACTGCAGAA+GGG 0.632959 contig294end:+16144 MsG0080048382.01.T01:intergenic
CTAACCTGAGTATAATTCAG+AGG 0.633689 contig294end:+17223 MsG0080048382.01.T01:intergenic
ATTCTGCGAGAAGGCCAGCA+TGG 0.649764 contig294end:-15713 MsG0080048382.01.T01:intron
AAAGCGTTGTGTACATGCCG+CGG 0.666394 contig294end:-15768 MsG0080048382.01.T01:CDS
TATATTGGGAGAGAAAGCTA+AGG 0.687081 contig294end:-17308 MsG0080048382.01.T01:CDS
TTTGCAGTCCAAGGCCACAA+CGG 0.687276 contig294end:-16186 MsG0080048382.01.T01:intron
GAAATCGACTACAACCGTTG+TGG 0.693445 contig294end:+16172 MsG0080048382.01.T01:intergenic
TTAGCTGTGACAAAGGCCAG+AGG 0.735270 contig294end:-16566 MsG0080048382.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGCAATTAATTATTAATT+AGG + contig294end:16300-16319 MsG0080048382.01.T01:intergenic 10.0%
!! AATTGATGTAAAAAATAAAT+GGG - contig294end:15896-15915 MsG0080048382.01.T01:intron 10.0%
!!! AAATTGTTTTATTGATTTTA+AGG - contig294end:16053-16072 MsG0080048382.01.T01:intron 10.0%
!!! AATTGTTTTATTGATTTTAA+GGG - contig294end:16054-16073 MsG0080048382.01.T01:intron 10.0%
!! AAATTGAAGTGTAAATTAAA+TGG - contig294end:18126-18145 MsG0080048382.01.T01:intron 15.0%
!! GAATTGATGTAAAAAATAAA+TGG - contig294end:15895-15914 MsG0080048382.01.T01:intron 15.0%
!!! AAATCAATAAAACAATTTTC+TGG + contig294end:16051-16070 MsG0080048382.01.T01:intergenic 15.0%
!!! AAATTCTGAAAAAACATTTT+TGG + contig294end:15992-16011 MsG0080048382.01.T01:intergenic 15.0%
!!! GTTTTTTCAGAATTTTTTTT+TGG - contig294end:15997-16016 MsG0080048382.01.T01:intron 15.0%
!! AAGATTGAGAATTTATTTAG+TGG - contig294end:18158-18177 MsG0080048382.01.T01:intron 20.0%
!! AGAGATAAAGTATGTTATAT+TGG - contig294end:16863-16882 MsG0080048382.01.T01:intron 20.0%
!! AGATTGAGAATTTATTTAGT+GGG - contig294end:18159-18178 MsG0080048382.01.T01:intron 20.0%
!! ATATAAGCACTTAATTAACA+TGG - contig294end:17782-17801 MsG0080048382.01.T01:intron 20.0%
!! CAATAACAATACTAATTACT+TGG - contig294end:15957-15976 MsG0080048382.01.T01:intron 20.0%
!! CTACAGGAAAATAAAAAATT+AGG + contig294end:17808-17827 MsG0080048382.01.T01:intergenic 20.0%
!! CTTCAATTTAAATGCATATT+TGG + contig294end:18115-18134 MsG0080048382.01.T01:intergenic 20.0%
!! GAGATAAAGTATGTTATATT+GGG - contig294end:16864-16883 MsG0080048382.01.T01:intron 20.0%
!! TCCTAAATTAAATTGTTTCA+AGG - contig294end:17038-17057 MsG0080048382.01.T01:intron 20.0%
!! TGAATTAGCAATAACAAAAT+TGG - contig294end:17917-17936 MsG0080048382.01.T01:intron 20.0%
!! TTAAAATATGAATGTTTGTC+CGG - contig294end:18854-18873 MsG0080048382.01.T01:intron 20.0%
!!! AAGGGTTTGATTTTTTTATT+AGG - contig294end:16072-16091 MsG0080048382.01.T01:intron 20.0%
!!! ACAAACATTCATATTTTAAG+TGG + contig294end:18853-18872 MsG0080048382.01.T01:intergenic 20.0%
!!! AGGGTTTGATTTTTTTATTA+GGG - contig294end:16073-16092 MsG0080048382.01.T01:intron 20.0%
!!! ATGTAAAAAATAAATGGGTT+GGG - contig294end:15901-15920 MsG0080048382.01.T01:intron 20.0%
!!! GTTTTTAAAGCAATTTTTCA+TGG - contig294end:18233-18252 MsG0080048382.01.T01:intron 20.0%
!!! TATGATAATGAACTTAATTC+TGG - contig294end:16217-16236 MsG0080048382.01.T01:intron 20.0%
!!! TCCTTGAAACAATTTAATTT+AGG + contig294end:17042-17061 MsG0080048382.01.T01:intergenic 20.0%
!!! TTATTTCTTCTTCACTATAT+AGG - contig294end:18377-18396 MsG0080048382.01.T01:intron 20.0%
!!! TTCAATTTTCGTTTTTGATT+TGG - contig294end:15519-15538 MsG0080048382.01.T01:intron 20.0%
!!! TTGTTTTGATTTTAAGAGAA+AGG - contig294end:16098-16117 MsG0080048382.01.T01:intron 20.0%
!!! TTTAATTGATGTTTTTTTCC+TGG - contig294end:15634-15653 MsG0080048382.01.T01:intron 20.0%
!!! TTTTATAGTTTAGAGTTGAT+TGG - contig294end:16403-16422 MsG0080048382.01.T01:intron 20.0%
! AAACCCAATCAAACAAAAAA+AGG + contig294end:15560-15579 MsG0080048382.01.T01:intergenic 25.0%
! AAACCTTATGACATTATACA+TGG + contig294end:16782-16801 MsG0080048382.01.T01:intergenic 25.0%
! AAATGCCAAAAAAGAACAAA+TGG + contig294end:18654-18673 MsG0080048382.01.T01:intergenic 25.0%
! AACCCAATCAAACAAAAAAA+GGG + contig294end:15559-15578 MsG0080048382.01.T01:intergenic 25.0%
! AACCTCTAATTTCTTTAATC+AGG - contig294end:16183-16202 MsG0080048382.01.T01:intron 25.0%
! AGTATATGACAATGTAAAAC+AGG - contig294end:18051-18070 MsG0080048382.01.T01:intron 25.0%
! AGTGAAGAAGAAATAATCAA+TGG + contig294end:18373-18392 MsG0080048382.01.T01:intergenic 25.0%
! AGTGTAAATTAAATGGTTCT+TGG - contig294end:18133-18152 MsG0080048382.01.T01:intron 25.0%
! ATAAAAATGAACAAAAGCAG+AGG + contig294end:17265-17284 MsG0080048382.01.T01:intergenic 25.0%
! ATAGTAAGGTCATTTAGTAT+AGG + contig294end:17861-17880 MsG0080048382.01.T01:intergenic 25.0%
! CATAATATAGTCTTCAATCT+TGG + contig294end:18294-18313 MsG0080048382.01.T01:intergenic 25.0%
! CTAGATATAAGTAAAACACA+AGG + contig294end:17123-17142 MsG0080048382.01.T01:intergenic 25.0%
! CTAGATATATGTAAAACACA+AGG + contig294end:17005-17024 MsG0080048382.01.T01:intergenic 25.0%
! CTGTAATAATATTGATCCTA+TGG + contig294end:18720-18739 MsG0080048382.01.T01:intergenic 25.0%
! GATGTAAAAAATAAATGGGT+TGG - contig294end:15900-15919 MsG0080048382.01.T01:intron 25.0%
! TAACCATGTATAATGTCATA+AGG - contig294end:16776-16795 MsG0080048382.01.T01:intron 25.0%
! TACCTGATTAAAGAAATTAG+AGG + contig294end:16188-16207 MsG0080048382.01.T01:intergenic 25.0%
! TAGAAAACAAAGTTTACAAG+TGG - contig294end:17140-17159 MsG0080048382.01.T01:intron 25.0%
! TATACTAAATGACCTTACTA+TGG - contig294end:17860-17879 MsG0080048382.01.T01:intron 25.0%
! TATTAAATGACAGAATCATC+AGG - contig294end:16319-16338 MsG0080048382.01.T01:CDS 25.0%
! TCAAGGAATATTGCAAAATA+TGG - contig294end:17055-17074 MsG0080048382.01.T01:intron 25.0%
! TCTTATACTGCAATACTAAA+TGG - contig294end:16730-16749 MsG0080048382.01.T01:intron 25.0%
! TTCTGTACTAAAATTCAACA+TGG + contig294end:17435-17454 MsG0080048382.01.T01:intergenic 25.0%
! TTTAGTATAGGTATTTCAAG+AGG + contig294end:17849-17868 MsG0080048382.01.T01:intergenic 25.0%
!!! AAACAAAAAAAGGGTATTTG+TGG + contig294end:15550-15569 MsG0080048382.01.T01:intergenic 25.0%
!!! AATCGATGGTTTTTTTTTTG+TGG - contig294end:15666-15685 MsG0080048382.01.T01:intron 25.0%
!!! ATACCCTTTTTTTGTTTGAT+TGG - contig294end:15553-15572 MsG0080048382.01.T01:intron 25.0%
!!! ATCGATGGTTTTTTTTTTGT+GGG - contig294end:15667-15686 MsG0080048382.01.T01:intron 25.0%
!!! ATTTGTTCTTTTTTGGCATT+TGG - contig294end:18653-18672 MsG0080048382.01.T01:intron 25.0%
!!! CTAAACCATTTGTTCTTTTT+TGG - contig294end:18646-18665 MsG0080048382.01.T01:intron 25.0%
!!! GAATTTTTTTTTGGTTTCGA+TGG - contig294end:16006-16025 MsG0080048382.01.T01:intron 25.0%
!!! TACCCTTTTTTTGTTTGATT+GGG - contig294end:15554-15573 MsG0080048382.01.T01:intron 25.0%
!!! TGTTGTGTCTTTATTTTAGA+AGG - contig294end:16141-16160 MsG0080048382.01.T01:CDS 25.0%
AACCATGTATAGATGTCATA+AGG - contig294end:16816-16835 MsG0080048382.01.T01:intron 30.0%
AAGCTTCAATTACTCAACAA+TGG + contig294end:15432-15451 MsG0080048382.01.T01:intergenic 30.0%
ACTATATAGGCAACATCATT+TGG - contig294end:18390-18409 MsG0080048382.01.T01:intron 30.0%
AGACACCAAAGTTTAATACA+TGG + contig294end:16647-16666 MsG0080048382.01.T01:intergenic 30.0%
AGGATCAATATTATTACAGC+AGG - contig294end:18721-18740 MsG0080048382.01.T01:intron 30.0%
ATGCTCCATGTATTAAACTT+TGG - contig294end:16639-16658 MsG0080048382.01.T01:intron 30.0%
ATTTCACATTACCTGAGATA+TGG + contig294end:17895-17914 MsG0080048382.01.T01:intergenic 30.0%
ATTTGGTTAACTATAACGTC+AGG - contig294end:18670-18689 MsG0080048382.01.T01:intron 30.0%
CAAGTCTACATTCTGTTTAT+AGG - contig294end:16599-16618 MsG0080048382.01.T01:CDS 30.0%
CTATTGATACTCAACATGTT+GGG - contig294end:17660-17679 MsG0080048382.01.T01:intron 30.0%
GATTCTTAATTAGCTAGCAA+TGG - contig294end:18181-18200 MsG0080048382.01.T01:intron 30.0%
GTATAGATGTCATAAGGAAA+GGG - contig294end:16822-16841 MsG0080048382.01.T01:intron 30.0%
TAGAGTATGAAATAACTGCA+CGG + contig294end:18593-18612 MsG0080048382.01.T01:intergenic 30.0%
TATTGATACTCAACATGTTG+GGG - contig294end:17661-17680 MsG0080048382.01.T01:intron 30.0%
TGTAAAACAGGTTTGAATTC+TGG - contig294end:18063-18082 MsG0080048382.01.T01:intron 30.0%
TGTATAGATGTCATAAGGAA+AGG - contig294end:16821-16840 MsG0080048382.01.T01:intron 30.0%
TTCCTTATGACATCTATACA+TGG + contig294end:16821-16840 MsG0080048382.01.T01:intergenic 30.0%
TTTGGTTAACTATAACGTCA+GGG - contig294end:18671-18690 MsG0080048382.01.T01:intron 30.0%
! AAATGACCTTACTATGGTTT+TGG - contig294end:17866-17885 MsG0080048382.01.T01:intron 30.0%
! AAGCAATTTTTCATGGCTAA+AGG - contig294end:18240-18259 MsG0080048382.01.T01:intron 30.0%
! AATGACCTTACTATGGTTTT+GGG - contig294end:17867-17886 MsG0080048382.01.T01:intron 30.0%
! ATATTGGACAGGTTTTTCTT+TGG - contig294end:17687-17706 MsG0080048382.01.T01:intron 30.0%
! TAGTTTAGAGTTGATTGGTT+TGG - contig294end:16408-16427 MsG0080048382.01.T01:intron 30.0%
! TATTGGACAGGTTTTTCTTT+GGG - contig294end:17688-17707 MsG0080048382.01.T01:intron 30.0%
! TGTAGAATTATGCTGTGTTT+TGG - contig294end:18337-18356 MsG0080048382.01.T01:intron 30.0%
! TGTTGTTTGTAATGTGAAGA+TGG - contig294end:17474-17493 MsG0080048382.01.T01:intron 30.0%
! TTTGGGAAATTCAAGTTCTA+GGG - contig294end:16372-16391 MsG0080048382.01.T01:intron 30.0%
!! AGTTTAGAGTTGATTGGTTT+GGG - contig294end:16409-16428 MsG0080048382.01.T01:intron 30.0%
!! TTTATTTTCCTGTAGCTTTG+TGG - contig294end:17813-17832 MsG0080048382.01.T01:CDS 30.0%
!! TTTTTCTTTGGGAGAGATTA+TGG - contig294end:17699-17718 MsG0080048382.01.T01:intron 30.0%
!!! GTTTTTTTTTTGTGGGTTTC+TGG - contig294end:15674-15693 MsG0080048382.01.T01:intron 30.0%
AAAGAAGATTGATGCAGATG+AGG - contig294end:15710-15729 MsG0080048382.01.T01:intron 35.0%
AATATGGACATTGCTCTTGA+TGG - contig294end:17071-17090 MsG0080048382.01.T01:intron 35.0%
ACAGAAGTTACAGAGAATCA+AGG - contig294end:15483-15502 MsG0080048382.01.T01:CDS 35.0%
ACATTGTCATATACTGCAGA+AGG + contig294end:18046-18065 MsG0080048382.01.T01:intergenic 35.0%
ACTTCAAGTGATAGGACAAT+AGG - contig294end:18268-18287 MsG0080048382.01.T01:intron 35.0%
AGGAAACAACTTCAAGTGAT+AGG - contig294end:18260-18279 MsG0080048382.01.T01:intron 35.0%
ATGGAAAGAAGAAGGAAGAA+GGG - contig294end:15748-15767 MsG0080048382.01.T01:CDS 35.0%
ATTACAACCTGTTCCACAAA+GGG + contig294end:17563-17582 MsG0080048382.01.T01:intergenic 35.0%
ATTCCACAATTTCAACGTTG+CGG + contig294end:18772-18791 MsG0080048382.01.T01:intergenic 35.0%
CATTGTCATATACTGCAGAA+GGG + contig294end:18045-18064 MsG0080048382.01.T01:intergenic 35.0%
CTAACCTGAGTATAATTCAG+AGG + contig294end:16966-16985 MsG0080048382.01.T01:intergenic 35.0%
CTTCAATTACTCAACAATGG+AGG + contig294end:15429-15448 MsG0080048382.01.T01:intergenic 35.0%
GAATCCTCTGAATTATACTC+AGG - contig294end:16959-16978 MsG0080048382.01.T01:intron 35.0%
GATTCTACTGTTTATGAGCT+TGG - contig294end:17406-17425 MsG0080048382.01.T01:intron 35.0%
GCTATTGATACTCAACATGT+TGG - contig294end:17659-17678 MsG0080048382.01.T01:intron 35.0%
GTGGAACAGGTTGTAATAAA+TGG - contig294end:17566-17585 MsG0080048382.01.T01:intron 35.0%
TATATTGGGAGAGAAAGCTA+AGG - contig294end:16878-16897 MsG0080048382.01.T01:intron 35.0%
TATTACAACCTGTTCCACAA+AGG + contig294end:17564-17583 MsG0080048382.01.T01:intergenic 35.0%
TCAACATACTTATATGCAGC+TGG - contig294end:18089-18108 MsG0080048382.01.T01:intron 35.0%
TGTAATAAATGGAGCAGCAA+AGG - contig294end:17577-17596 MsG0080048382.01.T01:intron 35.0%
TTATGATACCTGGATTCCAT+AGG + contig294end:17239-17258 MsG0080048382.01.T01:intergenic 35.0%
TTCTGGTAAGCACTTCTAAT+TGG - contig294end:16234-16253 MsG0080048382.01.T01:intron 35.0%
TTGTAATGTGAAGATGGAAC+AGG - contig294end:17480-17499 MsG0080048382.01.T01:intron 35.0%
! CACGATTTGGATTTGAGTTT+GGG - contig294end:16355-16374 MsG0080048382.01.T01:CDS 35.0%
! CCATCGATTAAGAAAAATCC+AGG + contig294end:15655-15674 MsG0080048382.01.T01:intergenic 35.0%
! CCTGGATTTTTCTTAATCGA+TGG - contig294end:15652-15671 MsG0080048382.01.T01:intron 35.0%
! GTTTGGGAAATTCAAGTTCT+AGG - contig294end:16371-16390 MsG0080048382.01.T01:intron 35.0%
! TCAATTTTCAACACCCTTTG+TGG - contig294end:17547-17566 MsG0080048382.01.T01:intron 35.0%
! TCACGATTTGGATTTGAGTT+TGG - contig294end:16354-16373 MsG0080048382.01.T01:CDS 35.0%
! TCATTTTCATGCAGTAGCTT+TGG - contig294end:17314-17333 MsG0080048382.01.T01:CDS 35.0%
! TCTTACATTTGCATTAGCCT+TGG + contig294end:18795-18814 MsG0080048382.01.T01:intergenic 35.0%
! TTTGGTTTCGATGGTGATTA+GGG - contig294end:16015-16034 MsG0080048382.01.T01:intron 35.0%
!! AAAAATAAATGGGTTGGGAG+TGG - contig294end:15906-15925 MsG0080048382.01.T01:intron 35.0%
!! AATGGAGGTTTTGTGAGTTA+TGG + contig294end:15414-15433 MsG0080048382.01.T01:intergenic 35.0%
!! ATGGAGGTTTTGTGAGTTAT+GGG + contig294end:15413-15432 MsG0080048382.01.T01:intergenic 35.0%
!! GAGAAAGGTGTTTGATTTGT+TGG - contig294end:16113-16132 MsG0080048382.01.T01:intron 35.0%
!! GAGTTGTGTCACTTTGTTTA+GGG - contig294end:16674-16693 MsG0080048382.01.T01:intron 35.0%
!! GTTTAGAGTTGATTGGTTTG+GGG - contig294end:16410-16429 MsG0080048382.01.T01:intron 35.0%
!! TGAGTTGTGTCACTTTGTTT+AGG - contig294end:16673-16692 MsG0080048382.01.T01:intron 35.0%
!!! TTTTGGTTTCGATGGTGATT+AGG - contig294end:16014-16033 MsG0080048382.01.T01:intron 35.0%
AATAAATGGAGCAGCAAAGG+AGG - contig294end:17580-17599 MsG0080048382.01.T01:intron 40.0%
ACACCTGATAAGATCACATC+CGG + contig294end:18876-18895 MsG0080048382.01.T01:intergenic 40.0%
ACAGAGAATCAAGGTGAGTT+GGG - contig294end:15492-15511 MsG0080048382.01.T01:CDS 40.0%
AGCAGTATTAGCTGTGACAA+AGG - contig294end:17613-17632 MsG0080048382.01.T01:intron 40.0%
CAACATGTTGGGGAAGATAT+TGG - contig294end:17671-17690 MsG0080048382.01.T01:intron 40.0%
CAATTTCAACGTTGCGGTAA+TGG + contig294end:18766-18785 MsG0080048382.01.T01:intergenic 40.0%
CGTTGAAATTGTGGAATCCA+AGG - contig294end:18775-18794 MsG0080048382.01.T01:intron 40.0%
GAGACATTACCTCAAACACT+TGG + contig294end:15938-15957 MsG0080048382.01.T01:intergenic 40.0%
GAGGAAAGAATTGATGCAGA+TGG - contig294end:15729-15748 MsG0080048382.01.T01:CDS 40.0%
GATGGAAAGAAGAAGGAAGA+AGG - contig294end:15747-15766 MsG0080048382.01.T01:CDS 40.0%
TAAACTCACGACAGGTAATG+AGG - contig294end:17201-17220 MsG0080048382.01.T01:intron 40.0%
TAATGCTGCAGATCACGATT+TGG - contig294end:16342-16361 MsG0080048382.01.T01:CDS 40.0%
TACAGAGAATCAAGGTGAGT+TGG - contig294end:15491-15510 MsG0080048382.01.T01:CDS 40.0%
TGATGCAGATGGAAAGAAGA+AGG - contig294end:15740-15759 MsG0080048382.01.T01:CDS 40.0%
TTACCGCAACGTTGAAATTG+TGG - contig294end:18766-18785 MsG0080048382.01.T01:intron 40.0%
TTTGATTCCCATAAAGCGTG+TGG - contig294end:17378-17397 MsG0080048382.01.T01:intron 40.0%
TTTGTGAGTTATGGGTGAGA+GGG + contig294end:15405-15424 MsG0080048382.01.T01:intergenic 40.0%
! ATGCAAGTTGAGGAAGAAGA+AGG + contig294end:15379-15398 MsG0080048382.01.T01:intergenic 40.0%
! ATTGATCCTATGGAAAGCGT+TGG + contig294end:18710-18729 MsG0080048382.01.T01:intergenic 40.0%
! TTTTGTGAGTTATGGGTGAG+AGG + contig294end:15406-15425 MsG0080048382.01.T01:intergenic 40.0%
!!! GATTGGTTTGGGGTTTGTTT+TGG - contig294end:16420-16439 MsG0080048382.01.T01:intron 40.0%
ACCGTATCCAATTTGATCCG+CGG + contig294end:18438-18457 MsG0080048382.01.T01:intergenic 45.0%
AGAAGAAGAGGAAACGGGTA+CGG - contig294end:15308-15327 MsG0080048382.01.T01:intron 45.0%
ATCCTGATCCACACGCTTTA+TGG + contig294end:17389-17408 MsG0080048382.01.T01:intergenic 45.0%
ATTGTCTGCATTCTGCGAGA+AGG - contig294end:18464-18483 MsG0080048382.01.T01:CDS 45.0%
GAAATCGACTACAACCGTTG+TGG + contig294end:18017-18036 MsG0080048382.01.T01:intergenic 45.0%
GAATTGCGATTTGCAGTCCA+AGG - contig294end:17991-18010 MsG0080048382.01.T01:intron 45.0%
GCAGAGGAGTTTATGATACC+TGG + contig294end:17249-17268 MsG0080048382.01.T01:intergenic 45.0%
GGGAATGCATTGTTCAAAGC+TGG - contig294end:15768-15787 MsG0080048382.01.T01:CDS 45.0%
GTCATCGATAAACTCACGAC+AGG - contig294end:17193-17212 MsG0080048382.01.T01:intron 45.0%
GTTTCGATGGTGATTAGGGT+TGG - contig294end:16019-16038 MsG0080048382.01.T01:intron 45.0%
TCCAGAAACTTCCATTCCTC+TGG + contig294end:17639-17658 MsG0080048382.01.T01:intergenic 45.0%
TCCTGATCCACACGCTTTAT+GGG + contig294end:17388-17407 MsG0080048382.01.T01:intergenic 45.0%
TGGAGCCCAAAACCATAGTA+AGG + contig294end:17875-17894 MsG0080048382.01.T01:intergenic 45.0%
TGTCCGGATGTGATCTTATC+AGG - contig294end:18870-18889 MsG0080048382.01.T01:intron 45.0%
TGTTGGGGAAGATATTGGAC+AGG - contig294end:17676-17695 MsG0080048382.01.T01:intron 45.0%
TTCAACACCCTTTGTGGAAC+AGG - contig294end:17553-17572 MsG0080048382.01.T01:intron 45.0%
! ACTATAACGTCAGGGTGTCT+TGG - contig294end:18679-18698 MsG0080048382.01.T01:intron 45.0%
! CAAGTTGAGGAAGAAGAAGG+AGG + contig294end:15376-15395 MsG0080048382.01.T01:intergenic 45.0%
!! TTTCCTGTAGCTTTGTGGCA+TGG - contig294end:17818-17837 MsG0080048382.01.T01:CDS 45.0%
AAACGGGTACGGGAGAAAGA+AGG - contig294end:15319-15338 MsG0080048382.01.T01:intron 50.0%
AACGGGTACGGGAGAAAGAA+GGG - contig294end:15320-15339 MsG0080048382.01.T01:intron 50.0%
AATGGAGCAGCAAAGGAGGT+AGG - contig294end:17584-17603 MsG0080048382.01.T01:intron 50.0%
CTTCCATGCCACAAAGCTAC+AGG + contig294end:17824-17843 MsG0080048382.01.T01:intergenic 50.0%
GAAGAAGAGGAAACGGGTAC+GGG - contig294end:15309-15328 MsG0080048382.01.T01:intron 50.0%
GCCAGAGGAATGGAAGTTTC+TGG - contig294end:17635-17654 MsG0080048382.01.T01:intron 50.0%
GCCGCGGATCAAATTGGATA+CGG - contig294end:18434-18453 MsG0080048382.01.T01:CDS 50.0%
GTACATGCCGCGGATCAAAT+TGG - contig294end:18428-18447 MsG0080048382.01.T01:CDS 50.0%
GTTGCTCCAACGCTTTCCAT+AGG - contig294end:18701-18720 MsG0080048382.01.T01:intron 50.0%
TCCCATAAAGCGTGTGGATC+AGG - contig294end:17384-17403 MsG0080048382.01.T01:intron 50.0%
TGGCACTCACCAAGTGTTTG+AGG - contig294end:15926-15945 MsG0080048382.01.T01:intron 50.0%
TGTGACAAAGGCCAGAGGAA+TGG - contig294end:17625-17644 MsG0080048382.01.T01:intron 50.0%
TTAGCTGTGACAAAGGCCAG+AGG - contig294end:17620-17639 MsG0080048382.01.T01:intron 50.0%
TTTGCAGTCCAAGGCCACAA+CGG - contig294end:18000-18019 MsG0080048382.01.T01:intron 50.0%
! GAGAGGGAGTATGCAAGTTG+AGG + contig294end:15389-15408 MsG0080048382.01.T01:intergenic 50.0%
!! AAAGCGTTGTGTACATGCCG+CGG - contig294end:18418-18437 MsG0080048382.01.T01:intron 50.0%
!!! GGTTTTGGGCTCCATATCTC+AGG - contig294end:17881-17900 MsG0080048382.01.T01:intron 50.0%
AAGGCCAGCATGGTGAGCAA+GGG - contig294end:18483-18502 MsG0080048382.01.T01:intron 55.0%
ACGGGTACGGGAGAAAGAAG+GGG - contig294end:15321-15340 MsG0080048382.01.T01:intron 55.0%
ATTCTGCGAGAAGGCCAGCA+TGG - contig294end:18473-18492 MsG0080048382.01.T01:CDS 55.0%
CTGAGTCGCCTATGGAATCC+AGG - contig294end:17228-17247 MsG0080048382.01.T01:CDS 55.0%
GACTACAACCGTTGTGGCCT+TGG + contig294end:18011-18030 MsG0080048382.01.T01:intergenic 55.0%
GAGGACTTCTGAGTCGCCTA+TGG - contig294end:17220-17239 MsG0080048382.01.T01:intron 55.0%
AGGCCAGCATGGTGAGCAAG+GGG - contig294end:18484-18503 MsG0080048382.01.T01:intron 60.0%
CGGGTACGGGAGAAAGAAGG+GGG - contig294end:15322-15341 MsG0080048382.01.T01:intron 60.0%
GAAGGCCAGCATGGTGAGCA+AGG - contig294end:18482-18501 MsG0080048382.01.T01:intron 60.0%
GGGTACGGGAGAAAGAAGGG+GGG - contig294end:15323-15342 MsG0080048382.01.T01:intron 60.0%
TCACCCCTTGCTCACCATGC+TGG + contig294end:18490-18509 MsG0080048382.01.T01:intergenic 60.0%
Chromosome Type Strat End Strand Name
contig294end gene 15305 18903 15305 ID=MsG0080048382.01;Name=MsG0080048382.01
contig294end mRNA 15305 18903 15305 ID=MsG0080048382.01.T01;Parent=MsG0080048382.01;Name=MsG0080048382.01.T01;_AED=0.48;_eAED=0.70;_QI=0|0|0|0.9|0.88|0.9|10|0|274
contig294end exon 18884 18903 18884 ID=MsG0080048382.01.T01:exon:37362;Parent=MsG0080048382.01.T01
contig294end exon 18419 18498 18419 ID=MsG0080048382.01.T01:exon:37361;Parent=MsG0080048382.01.T01
contig294end exon 17784 17876 17784 ID=MsG0080048382.01.T01:exon:37360;Parent=MsG0080048382.01.T01
contig294end exon 17228 17370 17228 ID=MsG0080048382.01.T01:exon:37359;Parent=MsG0080048382.01.T01
contig294end exon 16511 16633 16511 ID=MsG0080048382.01.T01:exon:37358;Parent=MsG0080048382.01.T01
contig294end exon 16306 16380 16306 ID=MsG0080048382.01.T01:exon:37357;Parent=MsG0080048382.01.T01
contig294end exon 16136 16201 16136 ID=MsG0080048382.01.T01:exon:37356;Parent=MsG0080048382.01.T01
contig294end exon 15714 15809 15714 ID=MsG0080048382.01.T01:exon:37355;Parent=MsG0080048382.01.T01
contig294end exon 15400 15516 15400 ID=MsG0080048382.01.T01:exon:37354;Parent=MsG0080048382.01.T01
contig294end exon 15305 15316 15305 ID=MsG0080048382.01.T01:exon:37353;Parent=MsG0080048382.01.T01
contig294end CDS 18884 18903 18884 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 18419 18498 18419 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 17784 17876 17784 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 17228 17370 17228 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 16511 16633 16511 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 16306 16380 16306 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 16136 16201 16136 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 15714 15809 15714 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 15400 15516 15400 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
contig294end CDS 15305 15316 15305 ID=MsG0080048382.01.T01:cds;Parent=MsG0080048382.01.T01
Gene Sequence

>MsG0080048382.01.T01

ATGAGAAGAAGAGGAAACGGAAAGAAGATTGATGCAGATGAGGAAAGAATTGATGCAGATGGAAAGAAGAAGGAAGAAGGGAATGCATTGTTCAAAGCTGAATCATCAGGTAATGCTGCAGATCACGATTTGGATTTGAGTTTGGGAAATTCAAGTTCTAGGGTTGATGCTTTTTATAGTTTAGAGTTGATTGGAAAGGGTGACTTCATATCTGATAAAGAGATAAAGTATGTTATATTGGGAGAGAAAGCTAAGGCTCAGTTGATTTGTGACTCAAAGAAAGACATTGCACTCAGTTTAACTGAAATACAAAAGAATCCTCTGAATTATACTCAGGTTGTAATAAATGGAGCAGCAAAGGAGGTAGGAAGAGCAGCAGTATTAGCTGTGACAAAGGCCAGAGGAATGGAAGTTTCTGGAGCTATTGATACTCAACATGTTGGGGAAGATATTGGACAGCTTTGTGGCATGGAAGAGCCTCTTGAAATACCTATACTAAATGACCTTACTATGGTTTTGGGCTCCATATCTCAGTCCAAGGCCACAACGGTTGTAGTCGATTTCACTGACCCTTCTGCAGTATATGACAATGTAAAACAGGCAACATCATTTGGCATGAAAAGCGTTGTGTACATGCCGCGGATCAAATTGGATACGGTTGCAGCATTGTCTGCATTCTGCGAGAAGGCCAGCATGGGTGTCTTGGTTGCTCCAACGCTTTCCATAGGATCAATATTATTACAGCAGGCTGCAATTTCAGCTTCTTTCCATTACCGCAACGTTGAAATTGTGGAATCCAAGGCTAATGCAAATGTGTTACGTTAA
Protein sequence

>MsG0080048382.01.T01

MRRRGNGKKIDADEERIDADGKKKEEGNALFKAESSGNAADHDLDLSLGNSSSRVDAFYSLELIGKGDFISDKEIKYVILGEKAKAQLICDSKKDIALSLTEIQKNPLNYTQVVINGAAKEVGRAAVLAVTKARGMEVSGAIDTQHVGEDIGQLCGMEEPLEIPILNDLTMVLGSISQSKATTVVVDFTDPSAVYDNVKQATSFGMKSVVYMPRIKLDTVAALSAFCEKASMGVLVAPTLSIGSILLQQAAISASFHYRNVEIVESKANANVLR*