Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048712.01.T01 | XP_013454626.1 | 98.204 | 167 | 3 | 0 | 13 | 179 | 29 | 195 | 2.21E-119 | 345 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048712.01.T01 | Q94AW5 | 57.927 | 164 | 52 | 3 | 13 | 176 | 29 | 175 | 1.03E-51 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048712.01.T01 | B0LL84 | 98.204 | 167 | 3 | 0 | 13 | 179 | 29 | 195 | 1.06e-119 | 345 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0080048712.01.T01 | TF | AP2/ERF-ERF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048712.01 | MsG0280008280.01 | 0.818072 | 2.372279e-52 | 1.757840e-49 |
| MsG0080048712.01 | MsG0280009514.01 | 0.834456 | 2.966041e-56 | 3.522021e-53 |
| MsG0080048712.01 | MsG0280011282.01 | 0.804428 | 2.170873e-49 | 1.118961e-46 |
| MsG0080048712.01 | MsG0480023196.01 | 0.808416 | 3.130802e-50 | 1.789108e-47 |
| MsG0080048712.01 | MsG0480023541.01 | 0.810820 | 9.531833e-51 | 5.804669e-48 |
| MsG0080048712.01 | MsG0580027208.01 | 0.808551 | 2.929226e-50 | 1.679852e-47 |
| MsG0080048712.01 | MsG0880044510.01 | 0.814073 | 1.854801e-51 | 1.232330e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048712.01.T01 | MTR_4g008860 | 98.204 | 167 | 3 | 0 | 13 | 179 | 29 | 195 | 2.68e-123 | 345 |
| MsG0080048712.01.T01 | MTR_3g106290 | 58.084 | 167 | 56 | 5 | 13 | 176 | 29 | 184 | 3.83e-43 | 142 |
| MsG0080048712.01.T01 | MTR_4g010640 | 51.316 | 76 | 22 | 1 | 13 | 73 | 28 | 103 | 1.04e-15 | 72.0 |
| MsG0080048712.01.T01 | MTR_4g114570 | 54.054 | 74 | 19 | 2 | 13 | 73 | 28 | 99 | 3.24e-15 | 70.9 |
| MsG0080048712.01.T01 | MTR_2g103700 | 59.701 | 67 | 21 | 1 | 13 | 73 | 28 | 94 | 3.28e-14 | 67.4 |
| MsG0080048712.01.T01 | MTR_2g014340 | 71.053 | 38 | 11 | 0 | 12 | 49 | 39 | 76 | 3.46e-13 | 65.1 |
| MsG0080048712.01.T01 | MTR_4g078710 | 70.000 | 40 | 12 | 0 | 12 | 51 | 48 | 87 | 3.71e-13 | 65.5 |
| MsG0080048712.01.T01 | MTR_3g053690 | 72.973 | 37 | 10 | 0 | 12 | 48 | 47 | 83 | 5.37e-12 | 62.4 |
| MsG0080048712.01.T01 | MTR_2g078680 | 59.322 | 59 | 18 | 2 | 12 | 65 | 51 | 108 | 6.94e-12 | 61.6 |
| MsG0080048712.01.T01 | MTR_7g084370 | 40.000 | 105 | 61 | 2 | 13 | 115 | 63 | 167 | 7.50e-12 | 62.4 |
| MsG0080048712.01.T01 | MTR_6g012970 | 56.667 | 60 | 21 | 2 | 12 | 70 | 58 | 113 | 3.80e-11 | 61.2 |
| MsG0080048712.01.T01 | MTR_2g014300 | 63.415 | 41 | 15 | 0 | 11 | 51 | 34 | 74 | 3.88e-11 | 59.3 |
| MsG0080048712.01.T01 | MTR_7g011630 | 52.542 | 59 | 26 | 1 | 12 | 70 | 62 | 118 | 4.55e-11 | 60.8 |
| MsG0080048712.01.T01 | MTR_2g105380 | 51.562 | 64 | 28 | 2 | 10 | 72 | 134 | 195 | 6.85e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048712.01.T01 | AT5G25190 | 57.927 | 164 | 52 | 3 | 13 | 176 | 29 | 175 | 1.05e-52 | 166 |
| MsG0080048712.01.T01 | AT1G15360 | 77.500 | 40 | 9 | 0 | 13 | 52 | 28 | 67 | 2.67e-14 | 68.2 |
| MsG0080048712.01.T01 | AT1G15360 | 77.500 | 40 | 9 | 0 | 13 | 52 | 28 | 67 | 2.67e-14 | 68.2 |
| MsG0080048712.01.T01 | AT5G25390 | 35.882 | 170 | 76 | 9 | 13 | 168 | 28 | 178 | 5.26e-13 | 64.7 |
| MsG0080048712.01.T01 | AT1G50640 | 65.000 | 40 | 14 | 0 | 11 | 50 | 47 | 86 | 3.22e-12 | 63.2 |
| MsG0080048712.01.T01 | AT3G20310 | 47.887 | 71 | 33 | 1 | 12 | 82 | 47 | 113 | 4.15e-12 | 62.8 |
| MsG0080048712.01.T01 | AT5G65130 | 35.971 | 139 | 62 | 7 | 9 | 136 | 128 | 250 | 4.67e-12 | 63.2 |
| MsG0080048712.01.T01 | AT5G65130 | 35.971 | 139 | 62 | 7 | 9 | 136 | 130 | 252 | 5.44e-12 | 63.2 |
| MsG0080048712.01.T01 | AT5G44210 | 44.156 | 77 | 38 | 2 | 12 | 88 | 53 | 124 | 6.62e-12 | 61.6 |
| MsG0080048712.01.T01 | AT5G13910 | 35.398 | 113 | 63 | 2 | 13 | 115 | 41 | 153 | 7.85e-12 | 61.6 |
| MsG0080048712.01.T01 | AT5G25390 | 55.385 | 65 | 21 | 2 | 16 | 72 | 28 | 92 | 1.11e-11 | 60.8 |
| MsG0080048712.01.T01 | AT3G15210 | 72.973 | 37 | 10 | 0 | 12 | 48 | 45 | 81 | 1.30e-11 | 61.2 |
| MsG0080048712.01.T01 | AT1G28360 | 65.789 | 38 | 13 | 0 | 11 | 48 | 31 | 68 | 4.82e-11 | 59.3 |
Find 26 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCAAGAGTTGCTTGATTA+TGG | 0.233710 | contig407end:+24677 | MsG0080048712.01.T01:CDS |
| ATTTGGGTTGTATGGGAAAT+TGG | 0.249663 | contig407end:-24327 | MsG0080048712.01.T01:intergenic |
| GGGAAATTGGTACGTGCTTT+TGG | 0.268347 | contig407end:-24314 | MsG0080048712.01.T01:intergenic |
| GTGAAACTAGTTTAATTAGA+TGG | 0.328749 | contig407end:+24555 | MsG0080048712.01.T01:CDS |
| GAAGAAGATTGTGGTCCATT+TGG | 0.328768 | contig407end:-24344 | MsG0080048712.01.T01:intergenic |
| TGTGGTCCATTTGGGTTGTA+TGG | 0.370819 | contig407end:-24335 | MsG0080048712.01.T01:intergenic |
| AATAATTGTGTTGGGTTTGA+AGG | 0.386713 | contig407end:+24590 | MsG0080048712.01.T01:CDS |
| TTGTGGTGGTTGTTGTTGTT+TGG | 0.389253 | contig407end:-24441 | MsG0080048712.01.T01:intergenic |
| TCAATTGAGCTTTGTTCTGT+TGG | 0.406867 | contig407end:+24701 | MsG0080048712.01.T01:CDS |
| ATGTGATCATCTTCAAGAAC+AGG | 0.413352 | contig407end:-24644 | MsG0080048712.01.T01:intergenic |
| AAGAAGATTGTGGTCCATTT+GGG | 0.419292 | contig407end:-24343 | MsG0080048712.01.T01:intergenic |
| ACTATAGGAAGACAAGAATA+TGG | 0.426325 | contig407end:+24222 | MsG0080048712.01.T01:CDS |
| TTAATTAGATGGCTTGATGA+AGG | 0.455274 | contig407end:+24566 | MsG0080048712.01.T01:CDS |
| ATGAAGGGAATAATTGTGTT+GGG | 0.501184 | contig407end:+24582 | MsG0080048712.01.T01:CDS |
| AATTTCCCATACAACCCAAA+TGG | 0.503068 | contig407end:+24329 | MsG0080048712.01.T01:CDS |
| AGGAAGACAAGAATATGGTT+AGG | 0.513155 | contig407end:+24227 | MsG0080048712.01.T01:CDS |
| GTGGTCCATTTGGGTTGTAT+GGG | 0.519874 | contig407end:-24334 | MsG0080048712.01.T01:intergenic |
| TAATTAGATGGCTTGATGAA+GGG | 0.525314 | contig407end:+24567 | MsG0080048712.01.T01:CDS |
| GATGAAGGGAATAATTGTGT+TGG | 0.532564 | contig407end:+24581 | MsG0080048712.01.T01:CDS |
| TTTGTCTATGGCAATGACAT+GGG | 0.635554 | contig407end:+24521 | MsG0080048712.01.T01:CDS |
| GTCTATGGCAATGACATGGG+TGG | 0.637989 | contig407end:+24524 | MsG0080048712.01.T01:CDS |
| AGCTTAGATGAAGAAGATTG+TGG | 0.644613 | contig407end:-24353 | MsG0080048712.01.T01:intergenic |
| AATGACATGGGTGGAACAAG+TGG | 0.664936 | contig407end:+24533 | MsG0080048712.01.T01:CDS |
| GAGCATATGATGAAGCAGCA+AGG | 0.668846 | contig407end:+24279 | MsG0080048712.01.T01:CDS |
| AAAGTTAAATAAGTGTCACA+TGG | 0.713555 | contig407end:+24397 | MsG0080048712.01.T01:CDS |
| GAAGCAGCAAGGTTAATGTG+TGG | 0.732232 | contig407end:+24290 | MsG0080048712.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGATGAATATTTTTTTT+TGG | + | contig407end:24199-24218 | MsG0080048712.01.T01:CDS | 10.0% |
| !!! | AATATTTTTTTTTGGACTAT+AGG | + | contig407end:24207-24226 | MsG0080048712.01.T01:CDS | 15.0% |
| ! | AAAGTTAAATAAGTGTCACA+TGG | + | contig407end:24397-24416 | MsG0080048712.01.T01:CDS | 25.0% |
| !! | AATAGTAACACTTTTGTCTA+TGG | + | contig407end:24509-24528 | MsG0080048712.01.T01:CDS | 25.0% |
| !! | GTGAAACTAGTTTAATTAGA+TGG | + | contig407end:24555-24574 | MsG0080048712.01.T01:CDS | 25.0% |
| AATAATTGTGTTGGGTTTGA+AGG | + | contig407end:24590-24609 | MsG0080048712.01.T01:CDS | 30.0% | |
| ACTATAGGAAGACAAGAATA+TGG | + | contig407end:24222-24241 | MsG0080048712.01.T01:CDS | 30.0% | |
| ATGAAGGGAATAATTGTGTT+GGG | + | contig407end:24582-24601 | MsG0080048712.01.T01:CDS | 30.0% | |
| ATTCAAGAGTTGCTTGATTA+TGG | + | contig407end:24677-24696 | MsG0080048712.01.T01:CDS | 30.0% | |
| TAATTAGATGGCTTGATGAA+GGG | + | contig407end:24567-24586 | MsG0080048712.01.T01:CDS | 30.0% | |
| TTAATTAGATGGCTTGATGA+AGG | + | contig407end:24566-24585 | MsG0080048712.01.T01:CDS | 30.0% | |
| ! | CTTTGTGTTGAAACTTTTTG+TGG | - | contig407end:24473-24492 | MsG0080048712.01.T01:intergenic | 30.0% |
| AAGAAGATTGTGGTCCATTT+GGG | - | contig407end:24346-24365 | MsG0080048711.01.T02:intergenic | 35.0% | |
| AATTTCCCATACAACCCAAA+TGG | + | contig407end:24329-24348 | MsG0080048712.01.T01:CDS | 35.0% | |
| AGCTTAGATGAAGAAGATTG+TGG | - | contig407end:24356-24375 | MsG0080048712.01.T01:intergenic | 35.0% | |
| AGGAAGACAAGAATATGGTT+AGG | + | contig407end:24227-24246 | MsG0080048712.01.T01:CDS | 35.0% | |
| ATGTGATCATCTTCAAGAAC+AGG | - | contig407end:24647-24666 | MsG0080048712.01.T01:intergenic | 35.0% | |
| GATGAAGGGAATAATTGTGT+TGG | + | contig407end:24581-24600 | MsG0080048712.01.T01:CDS | 35.0% | |
| TTTGTCTATGGCAATGACAT+GGG | + | contig407end:24521-24540 | MsG0080048712.01.T01:CDS | 35.0% | |
| ! | ACTTTTTGTGGCTCTTTTTG+TGG | - | contig407end:24461-24480 | MsG0080048712.01.T01:intergenic | 35.0% |
| ! | TTTTGTCTATGGCAATGACA+TGG | + | contig407end:24520-24539 | MsG0080048712.01.T01:CDS | 35.0% |
| !! | ATTTGGGTTGTATGGGAAAT+TGG | - | contig407end:24330-24349 | MsG0080048712.01.T01:intergenic | 35.0% |
| !! | TCAATTGAGCTTTGTTCTGT+TGG | + | contig407end:24701-24720 | MsG0080048712.01.T01:CDS | 35.0% |
| GAAGAAGATTGTGGTCCATT+TGG | - | contig407end:24347-24366 | MsG0080048712.01.T01:intergenic | 40.0% | |
| ! | TTGTGGTGGTTGTTGTTGTT+TGG | - | contig407end:24444-24463 | MsG0080048712.01.T01:intergenic | 40.0% |
| ! | TTTTGTGGCTCTTTTTGTGG+TGG | - | contig407end:24458-24477 | MsG0080048712.01.T01:intergenic | 40.0% |
| AATGACATGGGTGGAACAAG+TGG | + | contig407end:24533-24552 | MsG0080048712.01.T01:CDS | 45.0% | |
| GAAGCAGCAAGGTTAATGTG+TGG | + | contig407end:24290-24309 | MsG0080048712.01.T01:CDS | 45.0% | |
| GAGCATATGATGAAGCAGCA+AGG | + | contig407end:24279-24298 | MsG0080048712.01.T01:CDS | 45.0% | |
| ! | GGGAAATTGGTACGTGCTTT+TGG | - | contig407end:24317-24336 | MsG0080048712.01.T01:intergenic | 45.0% |
| !! | GTGGTCCATTTGGGTTGTAT+GGG | - | contig407end:24337-24356 | MsG0080048712.01.T01:intergenic | 45.0% |
| !! | TGTGGTCCATTTGGGTTGTA+TGG | - | contig407end:24338-24357 | MsG0080048712.01.T01:intergenic | 45.0% |
| GTCTATGGCAATGACATGGG+TGG | + | contig407end:24524-24543 | MsG0080048712.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig407end | gene | 24194 | 24733 | 24194 | ID=MsG0080048712.01;Name=MsG0080048712.01 |
| contig407end | mRNA | 24194 | 24733 | 24194 | ID=MsG0080048712.01.T01;Parent=MsG0080048712.01;Name=MsG0080048712.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|179 |
| contig407end | exon | 24194 | 24733 | 24194 | ID=MsG0080048712.01.T01:exon:1265;Parent=MsG0080048712.01.T01 |
| contig407end | CDS | 24194 | 24733 | 24194 | ID=MsG0080048712.01.T01:cds;Parent=MsG0080048712.01.T01 |
| Gene Sequence |
| Protein sequence |
