Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048812.01.T01 | XP_013466486.1 | 63 | 100 | 8 | 1 | 1 | 71 | 1 | 100 | 4.94E-22 | 92.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048812.01.T01 | A0A072VFF1 | 63.000 | 100 | 8 | 1 | 1 | 71 | 1 | 100 | 2.36e-22 | 92.8 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0080048812.01.T01 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048812.01 | MsG0180000332.01 | 0.813713 | 2.227188e-51 | 1.465386e-48 |
| MsG0080048812.01 | MsG0180001173.01 | 0.805822 | 1.108846e-49 | 5.924684e-47 |
| MsG0080048812.01 | MsG0180005443.01 | 0.807662 | 4.530131e-50 | 2.537885e-47 |
| MsG0080048812.01 | MsG0180005445.01 | 0.815162 | 1.064832e-51 | 7.285663e-49 |
| MsG0080048812.01 | MsG0280010447.01 | 0.801248 | 9.848614e-49 | 4.680920e-46 |
| MsG0080048812.01 | MsG0380012020.01 | 0.803025 | 4.244195e-49 | 2.110243e-46 |
| MsG0080048812.01 | MsG0480018462.01 | 0.802575 | 5.256547e-49 | 2.583884e-46 |
| MsG0080048812.01 | MsG0680031301.01 | 0.821886 | 3.180989e-53 | 2.620907e-50 |
| MsG0080048812.01 | MsG0780040845.01 | 0.805578 | 1.247542e-49 | 6.623910e-47 |
| MsG0080048812.01 | MsG0880047391.01 | 0.802210 | 6.249385e-49 | 3.043507e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048812.01.T01 | MTR_1g029990 | 63.000 | 100 | 8 | 1 | 1 | 71 | 1 | 100 | 5.99e-26 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGTTCTTCTTCTTTGC+AGG | 0.269891 | contig45end:+34868 | None:intergenic |
| CAGCGGTGGTTCAACGTTGC+CGG | 0.378236 | contig45end:+35100 | MsG0080048812.01.T01:CDS |
| GTTCGCCAGGTGGTGAAATC+AGG | 0.405267 | contig45end:+34907 | MsG0080048812.01.T01:CDS |
| CCACCGCTGGACGGCGGCGG+AGG | 0.407829 | contig45end:-35086 | None:intergenic |
| CGCTGGACGGCGGCGGAGGA+AGG | 0.429392 | contig45end:-35082 | None:intergenic |
| ATTTCACCACCTGGCGAACC+TGG | 0.448778 | contig45end:-34903 | None:intergenic |
| TGCGATGTCGGTACTGTGAT+CGG | 0.460955 | contig45end:+35129 | MsG0080048812.01.T01:CDS |
| TTACTGAGGGGTCATGGAGC+TGG | 0.465621 | contig45end:+35215 | MsG0080048812.01.T01:CDS |
| TACCGACATCGCAAGGCATC+CGG | 0.470871 | contig45end:-35119 | None:intergenic |
| TACAGTTGTTCACCACAGTA+AGG | 0.478529 | contig45end:-35178 | None:intergenic |
| TGCAGGAGGGTTAGAAAACC+AGG | 0.493973 | contig45end:+34885 | MsG0080048812.01.T01:CDS |
| GTTAGAAAACCAGGTTCGCC+AGG | 0.505813 | contig45end:+34894 | MsG0080048812.01.T01:CDS |
| GGAGGAAGGGAATGCGATTG+AGG | 0.508153 | contig45end:-35068 | None:intergenic |
| GAACCACCGCTGGACGGCGG+CGG | 0.514939 | contig45end:-35089 | None:intergenic |
| GCTGGACGGCGGCGGAGGAA+GGG | 0.522685 | contig45end:-35081 | None:intergenic |
| TGCCGGATGCCTTGCGATGT+CGG | 0.525213 | contig45end:+35117 | MsG0080048812.01.T01:CDS |
| CGGCAACGTTGAACCACCGC+TGG | 0.539612 | contig45end:-35099 | None:intergenic |
| ATGTTCTTCTTCTTTGCAGG+AGG | 0.539886 | contig45end:+34871 | MsG0080048812.01.T01:CDS |
| CCTCCGCCGCCGTCCAGCGG+TGG | 0.554775 | contig45end:+35086 | MsG0080048812.01.T01:CDS |
| TGTTCTTCTTCTTTGCAGGA+GGG | 0.566427 | contig45end:+34872 | MsG0080048812.01.T01:CDS |
| CCACACCTGATTTCACCACC+TGG | 0.568190 | contig45end:-34912 | None:intergenic |
| CTTCCTCCGCCGCCGTCCAG+CGG | 0.580854 | contig45end:+35083 | MsG0080048812.01.T01:CDS |
| AACGTTGAACCACCGCTGGA+CGG | 0.580920 | contig45end:-35095 | None:intergenic |
| CCAGGTGGTGAAATCAGGTG+TGG | 0.601448 | contig45end:+34912 | MsG0080048812.01.T01:CDS |
| ATCACAGTACCGACATCGCA+AGG | 0.607291 | contig45end:-35126 | None:intergenic |
| CAGGTGGTGAAATCAGGTGT+GGG | 0.626180 | contig45end:+34913 | MsG0080048812.01.T01:CDS |
| TGGTGAAATCAGGTGTGGGA+AGG | 0.629446 | contig45end:+34917 | MsG0080048812.01.T01:CDS |
| TCGGTACTGTGATCGGAATG+TGG | 0.638906 | contig45end:+35136 | MsG0080048812.01.T01:CDS |
| GTTGAACCACCGCTGGACGG+CGG | 0.641852 | contig45end:-35092 | None:intergenic |
| TTTACGTTCTGTCCTTACTG+TGG | 0.643662 | contig45end:+35166 | MsG0080048812.01.T01:CDS |
| AGAAAACCAGGTTCGCCAGG+TGG | 0.662890 | contig45end:+34897 | MsG0080048812.01.T01:CDS |
| GTACTGTGATCGGAATGTGG+CGG | 0.761724 | contig45end:+35139 | MsG0080048812.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACAAGAGATTTCCTTCCC+TGG | - | contig45end:35024-35043 | None:intergenic | 40.0% | |
| ATGTTCTTCTTCTTTGCAGG+AGG | + | contig45end:34871-34890 | MsG0080048812.01.T01:CDS | 40.0% | |
| TACAGTTGTTCACCACAGTA+AGG | - | contig45end:35181-35200 | None:intergenic | 40.0% | |
| TGTTCTTCTTCTTTGCAGGA+GGG | + | contig45end:34872-34891 | MsG0080048812.01.T01:CDS | 40.0% | |
| TTTACGTTCTGTCCTTACTG+TGG | + | contig45end:35166-35185 | MsG0080048812.01.T01:CDS | 40.0% | |
| !! | AACGCACTGTAGTTTTACTG+AGG | + | contig45end:35201-35220 | MsG0080048812.01.T01:CDS | 40.0% |
| !! | ACGCACTGTAGTTTTACTGA+GGG | + | contig45end:35202-35221 | MsG0080048812.01.T01:CDS | 40.0% |
| ! | AGCTTTTCTTCGTTCCCGTT+TGG | + | contig45end:34992-35011 | MsG0080048812.01.T01:intron | 45.0% |
| ! | TTTTCTTCGTTCCCGTTTGG+CGG | + | contig45end:34995-35014 | MsG0080048812.01.T01:intron | 45.0% |
| !! | CGCACTGTAGTTTTACTGAG+GGG | + | contig45end:35203-35222 | MsG0080048812.01.T01:CDS | 45.0% |
| !! | GTAGTTTTACTGAGGGGTCA+TGG | + | contig45end:35209-35228 | MsG0080048812.01.T01:CDS | 45.0% |
| CAGGTGGTGAAATCAGGTGT+GGG | + | contig45end:34913-34932 | MsG0080048812.01.T01:CDS | 50.0% | |
| GTACTGTGATCGGAATGTGG+CGG | + | contig45end:35139-35158 | MsG0080048812.01.T01:CDS | 50.0% | |
| GTTAGAAAACCAGGTTCGCC+AGG | + | contig45end:34894-34913 | MsG0080048812.01.T01:CDS | 50.0% | |
| TCGGTACTGTGATCGGAATG+TGG | + | contig45end:35136-35155 | MsG0080048812.01.T01:CDS | 50.0% | |
| TGCAGGAGGGTTAGAAAACC+AGG | + | contig45end:34885-34904 | MsG0080048812.01.T01:CDS | 50.0% | |
| TGCGATGTCGGTACTGTGAT+CGG | + | contig45end:35129-35148 | MsG0080048812.01.T01:CDS | 50.0% | |
| TGGTGAAATCAGGTGTGGGA+AGG | + | contig45end:34917-34936 | MsG0080048812.01.T01:CDS | 50.0% | |
| !! | ATCACAGTACCGACATCGCA+AGG | - | contig45end:35129-35148 | None:intergenic | 50.0% |
| AACGTTGAACCACCGCTGGA+CGG | - | contig45end:35098-35117 | None:intergenic | 55.0% | |
| AGAAAACCAGGTTCGCCAGG+TGG | + | contig45end:34897-34916 | MsG0080048812.01.T01:CDS | 55.0% | |
| ATTTCACCACCTGGCGAACC+TGG | - | contig45end:34906-34925 | None:intergenic | 55.0% | |
| CCACACCTGATTTCACCACC+TGG | - | contig45end:34915-34934 | None:intergenic | 55.0% | |
| CCAGGTGGTGAAATCAGGTG+TGG | + | contig45end:34912-34931 | MsG0080048812.01.T01:CDS | 55.0% | |
| GGAGGAAGGGAATGCGATTG+AGG | - | contig45end:35071-35090 | None:intergenic | 55.0% | |
| GTTCGCCAGGTGGTGAAATC+AGG | + | contig45end:34907-34926 | MsG0080048812.01.T01:CDS | 55.0% | |
| TTACTGAGGGGTCATGGAGC+TGG | + | contig45end:35215-35234 | MsG0080048812.01.T01:CDS | 55.0% | |
| ! | TACCGACATCGCAAGGCATC+CGG | - | contig45end:35122-35141 | None:intergenic | 55.0% |
| CAGCGGTGGTTCAACGTTGC+CGG | + | contig45end:35100-35119 | MsG0080048812.01.T01:CDS | 60.0% | |
| TCTTCGTTCCCGTTTGGCGG+TGG | + | contig45end:34998-35017 | MsG0080048812.01.T01:intron | 60.0% | |
| TGCCGGATGCCTTGCGATGT+CGG | + | contig45end:35117-35136 | MsG0080048812.01.T01:CDS | 60.0% | |
| CGGCAACGTTGAACCACCGC+TGG | - | contig45end:35102-35121 | None:intergenic | 65.0% | |
| CTTCCCTGGCCACCGCCAAA+CGG | - | contig45end:35010-35029 | None:intergenic | 65.0% | |
| GTTGAACCACCGCTGGACGG+CGG | - | contig45end:35095-35114 | None:intergenic | 65.0% | |
| TTCCCGTTTGGCGGTGGCCA+GGG | + | contig45end:35004-35023 | MsG0080048812.01.T01:intron | 65.0% | |
| TTCCCTGGCCACCGCCAAAC+GGG | - | contig45end:35009-35028 | None:intergenic | 65.0% | |
| CGTTTGGCGGTGGCCAGGGA+AGG | + | contig45end:35008-35027 | MsG0080048812.01.T01:intron | 70.0% | |
| GTTCCCGTTTGGCGGTGGCC+AGG | + | contig45end:35003-35022 | MsG0080048812.01.T01:intron | 70.0% | |
| CTTCCTCCGCCGCCGTCCAG+CGG | + | contig45end:35083-35102 | MsG0080048812.01.T01:CDS | 75.0% | |
| GAACCACCGCTGGACGGCGG+CGG | - | contig45end:35092-35111 | None:intergenic | 75.0% | |
| GCTGGACGGCGGCGGAGGAA+GGG | - | contig45end:35084-35103 | None:intergenic | 75.0% | |
| ! | CGCTGGACGGCGGCGGAGGA+AGG | - | contig45end:35085-35104 | None:intergenic | 80.0% |
| ! | CCACCGCTGGACGGCGGCGG+AGG | - | contig45end:35089-35108 | None:intergenic | 85.0% |
| ! | CCTCCGCCGCCGTCCAGCGG+TGG | + | contig45end:35086-35105 | MsG0080048812.01.T01:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig45end | gene | 34871 | 35241 | 34871 | ID=MsG0080048812.01;Name=MsG0080048812.01 |
| contig45end | mRNA | 34871 | 35241 | 34871 | ID=MsG0080048812.01.T01;Parent=MsG0080048812.01;Name=MsG0080048812.01.T01;_AED=0.50;_eAED=0.59;_QI=0|0|0|1|1|1|2|0|94 |
| contig45end | exon | 34871 | 34938 | 34871 | ID=MsG0080048812.01.T01:exon:345;Parent=MsG0080048812.01.T01 |
| contig45end | exon | 35025 | 35241 | 35025 | ID=MsG0080048812.01.T01:exon:346;Parent=MsG0080048812.01.T01 |
| contig45end | CDS | 34871 | 34938 | 34871 | ID=MsG0080048812.01.T01:cds;Parent=MsG0080048812.01.T01 |
| contig45end | CDS | 35025 | 35241 | 35025 | ID=MsG0080048812.01.T01:cds;Parent=MsG0080048812.01.T01 |
| Gene Sequence |
| Protein sequence |
