Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040845.01.T01 | XP_003625484.1 | 87.416 | 445 | 4 | 2 | 1 | 393 | 1 | 445 | 0 | 761 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0780040845.01 | 0.876553 | 1.274209e-68 | 6.203663e-65 |
| MsG0080048058.01 | MsG0780040845.01 | 0.861710 | 8.470855e-64 | 2.418249e-60 |
| MsG0080048383.01 | MsG0780040845.01 | 0.895974 | 5.752534e-76 | 6.097084e-72 |
| MsG0080048687.01 | MsG0780040845.01 | 0.877106 | 8.197944e-69 | 4.072926e-65 |
| MsG0080048765.01 | MsG0780040845.01 | 0.804244 | 2.370985e-49 | 1.216325e-46 |
| MsG0080048812.01 | MsG0780040845.01 | 0.805578 | 1.247542e-49 | 6.623910e-47 |
| MsG0080048815.01 | MsG0780040845.01 | 0.806749 | 7.071286e-50 | 3.868781e-47 |
| MsG0080048843.01 | MsG0780040845.01 | 0.811629 | 6.361774e-51 | 3.957680e-48 |
| MsG0080048855.01 | MsG0780040845.01 | 0.867883 | 9.853116e-66 | 3.494457e-62 |
| MsG0080048968.01 | MsG0780040845.01 | 0.817903 | 2.590561e-52 | 1.910724e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040845.01.T01 | MTR_7g099640 | 87.416 | 445 | 4 | 2 | 1 | 393 | 1 | 445 | 0.0 | 761 |
| MsG0780040845.01.T01 | MTR_1g069105 | 84.340 | 447 | 15 | 4 | 1 | 393 | 1 | 446 | 0.0 | 728 |
| MsG0780040845.01.T01 | MTR_7g099640 | 85.412 | 425 | 10 | 2 | 1 | 373 | 1 | 425 | 0.0 | 706 |
| MsG0780040845.01.T01 | MTR_1g069105 | 81.067 | 375 | 16 | 4 | 1 | 321 | 1 | 374 | 0.0 | 582 |
| MsG0780040845.01.T01 | MTR_3g062510 | 41.274 | 361 | 156 | 4 | 67 | 374 | 41 | 398 | 2.73e-90 | 278 |
| MsG0780040845.01.T01 | MTR_4g094270 | 40.157 | 381 | 165 | 5 | 65 | 385 | 30 | 407 | 6.11e-78 | 246 |
| MsG0780040845.01.T01 | MTR_1g083330 | 37.533 | 381 | 164 | 4 | 77 | 384 | 37 | 416 | 4.84e-76 | 242 |
| MsG0780040845.01.T01 | MTR_4g033235 | 36.579 | 380 | 168 | 3 | 78 | 384 | 38 | 417 | 9.16e-76 | 241 |
| MsG0780040845.01.T01 | MTR_3g117230 | 39.623 | 371 | 165 | 4 | 73 | 384 | 18 | 388 | 8.84e-75 | 238 |
| MsG0780040845.01.T01 | MTR_3g112260 | 38.547 | 358 | 168 | 2 | 79 | 384 | 28 | 385 | 9.20e-73 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040845.01.T01 | AT4G29040 | 83.146 | 445 | 21 | 4 | 1 | 393 | 1 | 443 | 0.0 | 717 |
| MsG0780040845.01.T01 | AT2G20140 | 82.921 | 445 | 22 | 4 | 1 | 393 | 1 | 443 | 0.0 | 716 |
| MsG0780040845.01.T01 | AT5G58290 | 42.265 | 362 | 153 | 4 | 66 | 374 | 33 | 391 | 2.01e-92 | 284 |
| MsG0780040845.01.T01 | AT1G09100 | 37.368 | 380 | 165 | 4 | 78 | 384 | 37 | 416 | 3.42e-75 | 240 |
| MsG0780040845.01.T01 | AT5G20000 | 42.154 | 325 | 135 | 2 | 114 | 385 | 85 | 409 | 3.75e-75 | 239 |
| MsG0780040845.01.T01 | AT3G05530 | 37.741 | 363 | 153 | 3 | 95 | 384 | 55 | 417 | 5.87e-75 | 239 |
| MsG0780040845.01.T01 | AT5G19990 | 41.846 | 325 | 136 | 2 | 114 | 385 | 27 | 351 | 6.65e-75 | 237 |
| MsG0780040845.01.T01 | AT5G19990 | 41.846 | 325 | 136 | 2 | 114 | 385 | 85 | 409 | 6.84e-75 | 239 |
| MsG0780040845.01.T01 | AT5G19990 | 41.846 | 325 | 136 | 2 | 114 | 385 | 85 | 409 | 6.84e-75 | 239 |
| MsG0780040845.01.T01 | AT1G45000 | 40.162 | 371 | 163 | 5 | 73 | 384 | 15 | 385 | 7.27e-74 | 236 |
Find 120 sgRNAs with CRISPR-Local
Find 369 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACACCGAAATGAAACAA+AGG | 0.594716 | 7:+83262332 | None:intergenic |
| AAAATCCCAACATAATACTC+CGG | 0.653134 | 7:+83267002 | None:intergenic |
| AAAGCAATATGTACCGAAGC+TGG | 0.494002 | 7:-83264113 | MsG0780040845.01.T01:CDS |
| AAAGGACAAGAAATTCGAAC+CGG | 0.554284 | 7:-83267330 | MsG0780040845.01.T01:CDS |
| AAAGGAGGCAGGGGTAAATG+AGG | 0.586817 | 7:+83262350 | None:intergenic |
| AAATGAAACAAAGGAGGCAG+GGG | 0.606531 | 7:+83262341 | None:intergenic |
| AAATTTAGATACATACATCT+AGG | 0.429419 | 7:-83264208 | MsG0780040845.01.T01:intron |
| AAGAAAGAAGGGGTGCCTGA+AGG | 0.613773 | 7:-83262394 | MsG0780040845.01.T01:CDS |
| AAGAGTGGAGTGAGAGAGAG+AGG | 0.442775 | 7:+83267461 | None:intergenic |
| AATCAAGGACTATTTGTTAA+TGG | 0.271847 | 7:-83267186 | MsG0780040845.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACACCGAAATGAAACAA+AGG | + | Chr7:83267260-83267279 | None:intergenic | 30.0% | |
| AAAATCCCAACATAATACTC+CGG | + | Chr7:83262590-83262609 | None:intergenic | 30.0% | |
| AAACATACAGTGTTCTAACA+AGG | + | Chr7:83266204-83266223 | None:intergenic | 30.0% | |
| AAATGAAGAAAAACGGTAAC+GGG | - | Chr7:83267475-83267494 | MsG0780040845.01.T01:five_prime_UTR | 30.0% | |
| AACATACAGTGTTCTAACAA+GGG | + | Chr7:83266203-83266222 | None:intergenic | 30.0% | |
| ACAGATCATAAGTAGATGAA+AGG | + | Chr7:83266426-83266445 | None:intergenic | 30.0% | |
| ACGGAATCATACTAAATATG+AGG | + | Chr7:83263593-83263612 | None:intergenic | 30.0% | |
| AGAAGAATTTGTCATGACTA+AGG | - | Chr7:83265430-83265449 | MsG0780040845.01.T01:intron | 30.0% | |
| AGGTGTAAATTGAAAAAGGT+TGG | + | Chr7:83265777-83265796 | None:intergenic | 30.0% | |
| AGTAACTTCTAACCTTCATT+CGG | + | Chr7:83265523-83265542 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 83262108 | 83267503 | 83262108 | ID=MsG0780040845.01;Name=MsG0780040845.01 |
| Chr7 | mRNA | 83262108 | 83267503 | 83262108 | ID=MsG0780040845.01.T01;Parent=MsG0780040845.01;Name=MsG0780040845.01.T01;_AED=0.49;_eAED=0.49;_QI=80|0.75|0.8|1|1|1|5|273|393 |
| Chr7 | exon | 83262108 | 83262464 | 83262108 | ID=MsG0780040845.01.T01:exon:19748;Parent=MsG0780040845.01.T01 |
| Chr7 | exon | 83264082 | 83264222 | 83264082 | ID=MsG0780040845.01.T01:exon:19747;Parent=MsG0780040845.01.T01 |
| Chr7 | exon | 83264323 | 83264539 | 83264323 | ID=MsG0780040845.01.T01:exon:19746;Parent=MsG0780040845.01.T01 |
| Chr7 | exon | 83265658 | 83265917 | 83265658 | ID=MsG0780040845.01.T01:exon:19745;Parent=MsG0780040845.01.T01 |
| Chr7 | exon | 83266944 | 83267503 | 83266944 | ID=MsG0780040845.01.T01:exon:19744;Parent=MsG0780040845.01.T01 |
| Chr7 | five_prime_UTR | 83267424 | 83267503 | 83267424 | ID=MsG0780040845.01.T01:five_prime_utr;Parent=MsG0780040845.01.T01 |
| Chr7 | CDS | 83266944 | 83267423 | 83266944 | ID=MsG0780040845.01.T01:cds;Parent=MsG0780040845.01.T01 |
| Chr7 | CDS | 83265658 | 83265917 | 83265658 | ID=MsG0780040845.01.T01:cds;Parent=MsG0780040845.01.T01 |
| Chr7 | CDS | 83264323 | 83264539 | 83264323 | ID=MsG0780040845.01.T01:cds;Parent=MsG0780040845.01.T01 |
| Chr7 | CDS | 83264082 | 83264222 | 83264082 | ID=MsG0780040845.01.T01:cds;Parent=MsG0780040845.01.T01 |
| Chr7 | CDS | 83262381 | 83262464 | 83262381 | ID=MsG0780040845.01.T01:cds;Parent=MsG0780040845.01.T01 |
| Chr7 | three_prime_UTR | 83262108 | 83262380 | 83262108 | ID=MsG0780040845.01.T01:three_prime_utr;Parent=MsG0780040845.01.T01 |
| Gene Sequence |
>MsG0780040845.01.T01 ATGGGTCAAGGAACACCAGGAGGCGGTTTGAACCGGCAACCGGGTGACCGGAAACCAGACGGCAACGACAAAAAGGACAAGAAATTCGAACCGGCGGCACCACCGGCTCGCGTCGGTCGCAAACAGCGAAAACAAAAGGGTTCCGAGGCTGCGTCGCGGTTGCCGACGGTAACTCCGTTATCGAAGTGCAAACTTCGGTTACTGAAACTGGAACGAATCAAGGACTATTTGTTAATGGAAGAAGAGTTTGTAGCGAATCAGGAAAGGTTGAAGCCTCAGGAGGAGAAAGCTGAAGAAGATAGATCTAAGGTTGATGATCTTCGTGGTTCACCTATGAGTGTTGGTAATCTTGAAGAGCTTATTGATGAGAATCATGCTATTGTTTCAAGTTCGGTTGGACCGGAGTATTATGTTGGGATTTTGTCCTTTGTTGATAAGGATCAATTGGAACCTGGTTGCGCTATTTTGATGCATAACAAGGTTCTTTCTGTTGTTGGACTTCTTCAAGATGAAGTTGATCCAATGGTCTCTGTAATGAAGGTTGAGAAGGCTCCTTTGGAATCATATGCTGACATTGGTGGTTTAGATGCCCAGATACAGGAAATTAAAGAAGCTGTAGAGCTTCCCCTTACACATCCTGAACTGTATGAAGATATTGGTATCAAGCCTCCAAAGGGAGTCATTTTATATGGAGAACCTGGAACCGGAAAGACACTTCTTGCAAAGGTGAGCATTTTTGTGTATGATGCTCACTCAGGTGGAGAGCGTGAAATTCAAAGGACCATGTTGGAGTTGCTGAACCAGTTAGATGGTTTTGATTCTAGAGGAGATGTTAAAGTTATTCTCGCAACCAACAGAATTGAAAGCCTTGATCCAGCTCTGCTAAGACCAGGTCGAATAGACAGGAAAATTGAATTTCCTCTCCCTGATATCAAAACTAGGAGACGCATTTTCACGATACATACATCTAGGATGACATTAGCTGACGATGTCAATTTAGAAGAATTTGTCATGACTAAGGATGAGTTCTCTGGAGCTGATATAAAAGCAATATGTACCGAAGCTGGCTTACTTGCTTTACGAGAACGCCGAATGAAGGTGACCCATGCTGATTTTAAGAAGGCAAAAGATAAAGTTATGTTTAAGAAGAAAGAAGGGGTGCCTGAAGGATTGTATATGTGA |
| Protein sequence |
>MsG0780040845.01.T01 MGQGTPGGGLNRQPGDRKPDGNDKKDKKFEPAAPPARVGRKQRKQKGSEAASRLPTVTPLSKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKVSIFVYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFTIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADFKKAKDKVMFKKKEGVPEGLYM* |
