AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000077.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000077.01.T01 MTR_1g024025 89.655 58 6 0 47 104 202 259 8.16e-28 107
MsG0180000077.01.T01 MTR_1g024025 96.296 27 1 0 113 139 330 356 4.36e-12 62.8
MsG0180000077.01.T01 MTR_3g064050 70.690 58 17 0 47 104 186 243 4.91e-20 85.5
MsG0180000077.01.T01 MTR_2g018690 68.966 58 18 0 47 104 183 240 2.74e-19 83.2
MsG0180000077.01.T01 MTR_5g076270 68.966 58 18 0 47 104 184 241 3.09e-19 83.2
MsG0180000077.01.T01 MTR_8g100050 60.656 61 24 0 47 107 218 278 5.62e-19 82.4
MsG0180000077.01.T01 MTR_2g005240 56.923 65 28 0 47 111 181 245 9.14e-19 81.6
MsG0180000077.01.T01 MTR_4g124900 71.698 53 15 0 47 99 182 234 1.07e-18 81.6
MsG0180000077.01.T01 MTR_4g124900 71.698 53 15 0 47 99 182 234 1.09e-18 81.6
MsG0180000077.01.T01 MTR_8g101360 74.510 51 13 0 47 97 191 241 1.15e-18 81.6
MsG0180000077.01.T01 MTR_8g100050 56.923 65 28 0 47 111 218 282 1.47e-18 81.3
MsG0180000077.01.T01 MTR_8g079492 67.241 58 19 0 47 104 100 157 1.88e-18 80.9
MsG0180000077.01.T01 MTR_8g079492 67.241 58 19 0 47 104 182 239 2.08e-18 80.9
MsG0180000077.01.T01 MTR_8g079492 67.241 58 19 0 47 104 182 239 2.08e-18 80.9
MsG0180000077.01.T01 MTR_2g043250 67.925 53 17 0 47 99 182 234 1.08e-17 78.6
MsG0180000077.01.T01 MTR_8g027440 60.345 58 23 0 47 104 187 244 4.27e-17 77.0
MsG0180000077.01.T01 MTR_5g074840 58.621 58 24 0 48 105 53 110 1.82e-15 69.3
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 94 151 3.00e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 224 281 3.02e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 224 281 3.02e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 237 294 3.06e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 94 151 3.13e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 107 164 3.23e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 237 294 3.24e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 237 294 3.24e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 224 281 3.26e-15 71.6
MsG0180000077.01.T01 MTR_2g093740 53.448 58 27 0 47 104 107 164 3.28e-15 71.6
MsG0180000077.01.T01 MTR_4g060460 53.448 58 27 0 47 104 222 279 1.03e-14 70.1
MsG0180000077.01.T01 MTR_4g060460 53.448 58 27 0 47 104 222 279 1.04e-14 70.1
MsG0180000077.01.T01 MTR_7g062540 56.897 58 25 0 47 104 172 229 1.15e-14 70.1
MsG0180000077.01.T01 MTR_4g021580 65.306 49 17 0 47 95 177 225 1.81e-14 69.3
MsG0180000077.01.T01 MTR_4g021580 65.306 49 17 0 47 95 177 225 1.81e-14 69.3
MsG0180000077.01.T01 MTR_7g101280 61.364 44 17 0 52 95 192 235 1.81e-12 63.5
MsG0180000077.01.T01 MTR_0041s0110 61.364 44 17 0 52 95 192 235 1.81e-12 63.5
MsG0180000077.01.T01 MTR_2g014770 58.696 46 19 0 47 92 214 259 2.69e-12 63.2
MsG0180000077.01.T01 MTR_2g014770 58.696 46 19 0 47 92 214 259 2.83e-12 63.2
MsG0180000077.01.T01 MTR_4g088210 58.696 46 19 0 47 92 204 249 3.00e-12 63.2
MsG0180000077.01.T01 MTR_4g088210 58.696 46 19 0 47 92 204 249 3.01e-12 63.2
MsG0180000077.01.T01 MTR_7g101275 61.364 44 17 0 52 95 153 196 1.19e-11 61.2
MsG0180000077.01.T01 MTR_2g094570 62.791 43 16 0 52 94 169 211 1.83e-11 60.8
MsG0180000077.01.T01 MTR_2g094570 62.791 43 16 0 52 94 225 267 1.88e-11 60.8
MsG0180000077.01.T01 MTR_2g094570 62.791 43 16 0 52 94 169 211 1.89e-11 60.8
MsG0180000077.01.T01 MTR_4g058930 62.791 43 16 0 52 94 170 212 3.53e-11 60.1
MsG0180000077.01.T01 MTR_4g058930 62.791 43 16 0 52 94 170 212 3.65e-11 60.1
MsG0180000077.01.T01 MTR_1g064430 60.465 43 17 0 52 94 190 232 5.47e-11 59.3
MsG0180000077.01.T01 MTR_1g064430 60.465 43 17 0 52 94 203 245 5.53e-11 59.3
MsG0180000077.01.T01 MTR_1g094960 60.465 43 17 0 52 94 179 221 8.00e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000077.01.T01 AT1G19850 47.097 155 20 3 47 139 214 368 9.21e-32 119
MsG0180000077.01.T01 AT1G19850 47.097 155 20 3 47 139 203 357 9.75e-32 119
MsG0180000077.01.T01 AT1G59750 62.295 61 23 0 47 107 180 240 5.71e-20 85.1
MsG0180000077.01.T01 AT1G59750 62.295 61 23 0 47 107 180 240 5.72e-20 85.1
MsG0180000077.01.T01 AT1G59750 62.295 61 23 0 47 107 180 240 5.72e-20 85.1
MsG0180000077.01.T01 AT1G59750 62.295 61 23 0 47 107 180 240 6.11e-20 85.1
MsG0180000077.01.T01 AT1G19220 73.585 53 14 0 47 99 182 234 1.30e-19 84.3
MsG0180000077.01.T01 AT1G30330 68.966 58 18 0 47 104 185 242 1.54e-19 84.0
MsG0180000077.01.T01 AT1G30330 68.966 58 18 0 47 104 185 242 1.54e-19 84.0
MsG0180000077.01.T01 AT1G30330 68.966 58 18 0 47 104 183 240 1.60e-19 84.0
MsG0180000077.01.T01 AT5G37020 67.241 58 19 0 47 104 182 239 4.24e-19 82.8
MsG0180000077.01.T01 AT5G37020 67.241 58 19 0 47 104 182 239 4.27e-19 82.8
MsG0180000077.01.T01 AT5G37020 67.241 58 19 0 47 104 87 144 4.54e-19 82.8
MsG0180000077.01.T01 AT5G62000 60.656 61 24 0 47 107 220 280 5.85e-19 82.4
MsG0180000077.01.T01 AT5G62000 60.656 61 24 0 47 107 220 280 5.85e-19 82.4
MsG0180000077.01.T01 AT5G62000 60.656 61 24 0 47 107 220 280 5.85e-19 82.4
MsG0180000077.01.T01 AT5G62000 60.656 61 24 0 47 107 220 280 5.85e-19 82.4
MsG0180000077.01.T01 AT5G62000 60.656 61 24 0 47 107 220 280 6.96e-19 82.0
MsG0180000077.01.T01 AT5G20730 71.698 53 15 0 47 99 183 235 2.09e-18 80.9
MsG0180000077.01.T01 AT5G20730 71.698 53 15 0 47 99 183 235 2.29e-18 80.9
MsG0180000077.01.T01 AT5G20730 71.698 53 15 0 47 99 183 235 2.29e-18 80.9
MsG0180000077.01.T01 AT2G33860 33.117 154 42 3 47 139 215 368 2.30e-18 80.5
MsG0180000077.01.T01 AT3G61830 62.069 58 22 0 47 104 133 190 3.73e-18 80.1
MsG0180000077.01.T01 AT3G61830 62.069 58 22 0 47 104 184 241 3.80e-18 80.1
MsG0180000077.01.T01 AT2G46530 62.069 58 22 0 47 104 180 237 4.35e-18 79.7
MsG0180000077.01.T01 AT2G46530 62.069 58 22 0 47 104 93 150 1.64e-17 78.2
MsG0180000077.01.T01 AT2G46530 62.069 58 22 0 47 104 201 258 1.68e-17 78.2
MsG0180000077.01.T01 AT5G60450 31.373 153 45 2 47 139 209 361 1.27e-16 75.9
MsG0180000077.01.T01 AT5G60450 31.373 153 45 2 47 139 233 385 1.36e-16 75.5
MsG0180000077.01.T01 AT1G34310 58.182 55 23 0 52 106 187 241 8.46e-16 73.2
MsG0180000077.01.T01 AT4G23980 68.000 50 16 0 47 96 172 221 2.36e-15 72.0
MsG0180000077.01.T01 AT4G23980 68.000 50 16 0 47 96 172 221 2.43e-15 72.0
MsG0180000077.01.T01 AT1G35520 49.275 69 31 1 52 120 187 251 5.42e-15 70.9
MsG0180000077.01.T01 AT1G35540 47.619 63 33 0 47 109 182 244 1.27e-14 70.1
MsG0180000077.01.T01 AT1G34410 47.826 69 32 1 52 120 187 251 1.62e-14 69.7
MsG0180000077.01.T01 AT1G35240 48.529 68 31 1 52 119 187 250 3.71e-14 68.6
MsG0180000077.01.T01 AT2G33860 53.846 52 24 0 47 98 58 109 1.25e-12 64.3
MsG0180000077.01.T01 AT1G34390 60.000 45 18 0 52 96 185 229 1.99e-12 63.5
MsG0180000077.01.T01 AT2G28350 62.791 43 16 0 52 94 176 218 1.92e-11 60.8
MsG0180000077.01.T01 AT2G28350 62.791 43 16 0 52 94 176 218 2.04e-11 60.8
MsG0180000077.01.T01 AT4G30080 62.791 43 16 0 52 94 181 223 5.82e-11 59.3

Find 27 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCATTGAAACAGGTAAAA+AGG 0.280677 1:-1178038 None:intergenic
TTCTAAGTGGCGAAATATTC+AGG 0.333372 1:+1178682 MsG0180000077.01.T01:CDS
GTTTGTTTGTCGGCTCAAAA+AGG 0.375508 1:+1178194 MsG0180000077.01.T01:CDS
CACAGGTTGTCAACAACAAT+CGG 0.399626 1:+1178014 MsG0180000077.01.T01:CDS
AACAATAATAGCATTGAAAC+AGG 0.406401 1:-1178046 None:intergenic
CTTAGAACCAGGCCACCTTA+AGG 0.409021 1:-1178666 None:intergenic
TTAGAACCAGGCCACCTTAA+GGG 0.411042 1:-1178665 None:intergenic
CGCAATTGCGAGTTGGTGTA+AGG 0.454613 1:+1178537 MsG0180000077.01.T01:CDS
GTCGATCCCTTAAGGTGGCC+TGG 0.457511 1:+1178659 MsG0180000077.01.T01:CDS
AAGAGACACCTTCTCACAAC+GGG 0.474222 1:+1178166 MsG0180000077.01.T01:CDS
AGATATATGGGTACAATTGT+TGG 0.476234 1:+1178626 MsG0180000077.01.T01:CDS
ACGGGTTGGAGTTTGTTTGT+CGG 0.492443 1:+1178184 MsG0180000077.01.T01:CDS
TGTATATCAACTACTGATGA+TGG 0.494800 1:-1178587 None:intergenic
TGGTAAAGATTCTGGTAGGA+AGG 0.523481 1:-1177962 None:intergenic
TTAGTGATGTCGATCCCTTA+AGG 0.537210 1:+1178651 MsG0180000077.01.T01:CDS
CGATTGTTGTTGACAACCTG+TGG 0.556740 1:-1178013 None:intergenic
AACTCCAACCCGTTGTGAGA+AGG 0.573060 1:-1178174 None:intergenic
ATATTTCGCCACTTAGAACC+AGG 0.586883 1:-1178677 None:intergenic
GACACCTTCTCACAACGGGT+TGG 0.588416 1:+1178170 MsG0180000077.01.T01:CDS
ACAGGTTGTCAACAACAATC+GGG 0.588604 1:+1178015 MsG0180000077.01.T01:CDS
TCTATCTAAGAATTCTGGGA+TGG 0.590743 1:+1177921 None:intergenic
GAGAAGTCGCAATTGCGAGT+TGG 0.615554 1:+1178530 MsG0180000077.01.T01:CDS
ATGATGGCAGTGTTGTCTGT+TGG 0.631904 1:-1178571 None:intergenic
TAAGGTGGCCTGGTTCTAAG+TGG 0.634814 1:+1178669 MsG0180000077.01.T01:CDS
TTCTTGGTAAAGATTCTGGT+AGG 0.647899 1:-1177966 None:intergenic
GAAGAGACACCTTCTCACAA+CGG 0.684005 1:+1178165 MsG0180000077.01.T01:CDS
GTGATGTCGATCCCTTAAGG+TGG 0.694797 1:+1178654 MsG0180000077.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAGACTAATTATTTTGA+AGG - Chr1:1178099-1178118 None:intergenic 15.0%
! CTCTTATGTTATCTGAATAT+AGG + Chr1:1178300-1178319 MsG0180000077.01.T01:intron 25.0%
! TGTTATGTAGAGATGATTAT+TGG - Chr1:1178473-1178492 None:intergenic 25.0%
!! AACAATAATAGCATTGAAAC+AGG - Chr1:1178049-1178068 None:intergenic 25.0%
!! AATGTAATGGTTTTCGATAT+TGG + Chr1:1178401-1178420 MsG0180000077.01.T01:intron 25.0%
!! ATGTAATGGTTTTCGATATT+GGG + Chr1:1178402-1178421 MsG0180000077.01.T01:intron 25.0%
AGATATATGGGTACAATTGT+TGG + Chr1:1178626-1178645 MsG0180000077.01.T01:CDS 30.0%
TGACCTACATGAAAAATGTA+AGG - Chr1:1178142-1178161 None:intergenic 30.0%
TGATGTGTCAATTGTTTCAA+GGG + Chr1:1178505-1178524 MsG0180000077.01.T01:intron 30.0%
TGTATATCAACTACTGATGA+TGG - Chr1:1178590-1178609 None:intergenic 30.0%
TTTCGATATTGGGAAAATTG+TGG + Chr1:1178412-1178431 MsG0180000077.01.T01:intron 30.0%
! ACATCTTCATCATGTTTTCT+TGG - Chr1:1177985-1178004 None:intergenic 30.0%
! CTCTTTTTCTGCAGATATAT+GGG + Chr1:1178614-1178633 MsG0180000077.01.T01:CDS 30.0%
! GAAGATGTTTTATAAACCAC+AGG + Chr1:1177997-1178016 MsG0180000077.01.T01:CDS 30.0%
! GATCCTTACATTTTTCATGT+AGG + Chr1:1178136-1178155 MsG0180000077.01.T01:intron 30.0%
! TAGCATTGAAACAGGTAAAA+AGG - Chr1:1178041-1178060 None:intergenic 30.0%
! TGTTTTCTTGGTAAAGATTC+TGG - Chr1:1177973-1177992 None:intergenic 30.0%
!!! AGACTTGTTTTTTTTCTCCA+TGG + Chr1:1178369-1178388 MsG0180000077.01.T01:intron 30.0%
ATGGTGACATTGCAATGTAA+TGG + Chr1:1178388-1178407 MsG0180000077.01.T01:intron 35.0%
ATTACATTGCAATGTCACCA+TGG - Chr1:1178389-1178408 None:intergenic 35.0%
CCATATATCTGCAGAAAAAG+AGG - Chr1:1178616-1178635 None:intergenic 35.0%
GTGATGTGTCAATTGTTTCA+AGG + Chr1:1178504-1178523 MsG0180000077.01.T01:intron 35.0%
TGTAAGGATCTAACTCAATC+AGG - Chr1:1178126-1178145 None:intergenic 35.0%
TTCTAAGTGGCGAAATATTC+AGG + Chr1:1178682-1178701 MsG0180000077.01.T01:CDS 35.0%
TTCTTGGTAAAGATTCTGGT+AGG - Chr1:1177969-1177988 None:intergenic 35.0%
! CCTCTTTTTCTGCAGATATA+TGG + Chr1:1178613-1178632 MsG0180000077.01.T01:CDS 35.0%
! GCGATTCTGTTCTTTTTATC+AGG + Chr1:1178227-1178246 MsG0180000077.01.T01:CDS 35.0%
ACAGGTTGTCAACAACAATC+GGG + Chr1:1178015-1178034 MsG0180000077.01.T01:CDS 40.0%
ATATTTCGCCACTTAGAACC+AGG - Chr1:1178680-1178699 None:intergenic 40.0%
CACAGGTTGTCAACAACAAT+CGG + Chr1:1178014-1178033 MsG0180000077.01.T01:CDS 40.0%
TGGTAAAGATTCTGGTAGGA+AGG - Chr1:1177965-1177984 None:intergenic 40.0%
TTAGTGATGTCGATCCCTTA+AGG + Chr1:1178651-1178670 MsG0180000077.01.T01:CDS 40.0%
!! GTTTGTTTGTCGGCTCAAAA+AGG + Chr1:1178194-1178213 MsG0180000077.01.T01:CDS 40.0%
AAGAGACACCTTCTCACAAC+GGG + Chr1:1178166-1178185 MsG0180000077.01.T01:CDS 45.0%
ATGATGGCAGTGTTGTCTGT+TGG - Chr1:1178574-1178593 None:intergenic 45.0%
GAAGAGACACCTTCTCACAA+CGG + Chr1:1178165-1178184 MsG0180000077.01.T01:CDS 45.0%
GGCTCAAAAAGGCTTAAAGC+AGG + Chr1:1178205-1178224 MsG0180000077.01.T01:CDS 45.0%
TTAGAACCAGGCCACCTTAA+GGG - Chr1:1178668-1178687 None:intergenic 45.0%
! CGATTGTTGTTGACAACCTG+TGG - Chr1:1178016-1178035 None:intergenic 45.0%
!! ACGGGTTGGAGTTTGTTTGT+CGG + Chr1:1178184-1178203 MsG0180000077.01.T01:CDS 45.0%
AACTCCAACCCGTTGTGAGA+AGG - Chr1:1178177-1178196 None:intergenic 50.0%
CTTAGAACCAGGCCACCTTA+AGG - Chr1:1178669-1178688 None:intergenic 50.0%
GAGAAGTCGCAATTGCGAGT+TGG + Chr1:1178530-1178549 MsG0180000077.01.T01:CDS 50.0%
GTGATGTCGATCCCTTAAGG+TGG + Chr1:1178654-1178673 MsG0180000077.01.T01:CDS 50.0%
!! CGCAATTGCGAGTTGGTGTA+AGG + Chr1:1178537-1178556 MsG0180000077.01.T01:CDS 50.0%
!! TAAGGTGGCCTGGTTCTAAG+TGG + Chr1:1178669-1178688 MsG0180000077.01.T01:CDS 50.0%
GACACCTTCTCACAACGGGT+TGG + Chr1:1178170-1178189 MsG0180000077.01.T01:CDS 55.0%
GTCGATCCCTTAAGGTGGCC+TGG + Chr1:1178659-1178678 MsG0180000077.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 1177935 1178712 1177935 ID=MsG0180000077.01;Name=MsG0180000077.01
Chr1 mRNA 1177935 1178712 1177935 ID=MsG0180000077.01.T01;Parent=MsG0180000077.01;Name=MsG0180000077.01.T01;_AED=0.58;_eAED=0.58;_QI=0|0|0|1|0|0.33|3|0|140
Chr1 exon 1177935 1178079 1177935 ID=MsG0180000077.01.T01:exon:7426;Parent=MsG0180000077.01.T01
Chr1 exon 1178158 1178248 1178158 ID=MsG0180000077.01.T01:exon:7425;Parent=MsG0180000077.01.T01
Chr1 exon 1178526 1178712 1178526 ID=MsG0180000077.01.T01:exon:7424;Parent=MsG0180000077.01.T01
Chr1 CDS 1177935 1178079 1177935 ID=MsG0180000077.01.T01:cds;Parent=MsG0180000077.01.T01
Chr1 CDS 1178158 1178248 1178158 ID=MsG0180000077.01.T01:cds;Parent=MsG0180000077.01.T01
Chr1 CDS 1178526 1178712 1178526 ID=MsG0180000077.01.T01:cds;Parent=MsG0180000077.01.T01
Gene Sequence

>MsG0180000077.01.T01

CTGGGATGGCGAAATATTTGTTCACAACCTTCCTACCAGAATCTTTACCAAGAAAACATGATGAAGATGTTTTATAAACCACAGGTTGTCAACAACAATCGGGCCTTTTTACCTGTTTCAATGCTATTATTGTTTGAGTTTCAATGTCAGCTGAAGAGACACCTTCTCACAACGGGTTGGAGTTTGTTTGTCGGCTCAAAAAGGCTTAAAGCAGGCGATTCTGTTCTTTTTATCAGGGACGAGAAGTCGCAATTGCGAGTTGGTGTAAGGCGTGCTAATCGCCAACAGACAACACTGCCATCATCAGTAGTTGATATACATTTCCTCTTTTTCTGCAGATATATGGGTACAATTGTTGGTATTAGTGATGTCGATCCCTTAAGGTGGCCTGGTTCTAAGTGGCGAAATATTCAGGTAGCTTAA

Protein sequence

>MsG0180000077.01.T01

LGWRNICSQPSYQNLYQENMMKMFYKPQVVNNNRAFLPVSMLLLFEFQCQLKRHLLTTGWSLFVGSKRLKAGDSVLFIRDEKSQLRVGVRRANRQQTTLPSSVVDIHFLFFCRYMGTIVGISDVDPLRWPGSKWRNIQVA*