AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025093.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025093.01.T01 MTR_5g022010 92.833 293 17 2 1 293 1 289 1.03e-180 499
MsG0580025093.01.T01 MTR_8g075950 50.571 350 100 14 1 288 1 339 9.58e-73 227
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025093.01.T01 AT5G66230 40.346 347 128 14 1 286 1 329 9.57e-45 154
MsG0580025093.01.T01 AT5G66230 39.769 347 130 14 1 286 217 545 1.22e-41 151
MsG0580025093.01.T01 AT3G51230 44.954 109 39 3 1 91 1 106 6.00e-18 80.5

Find 59 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAAATTCAGAGTCTTTC+TGG 0.117151 5:-13781229 MsG0580025093.01.T01:CDS
AAACGAAGATGTTTCCCTTT+TGG 0.292026 5:+13780184 None:intergenic
TCACCAATTGTGGGTCTTGC+TGG 0.314090 5:-13781379 MsG0580025093.01.T01:CDS
GTGAGAGTTGTTGTGGATGA+TGG 0.320301 5:+13781660 None:intergenic
ATGATGGCAGGTGGTGAAAA+TGG 0.338531 5:-13780662 MsG0580025093.01.T01:intron
TCAAGATCGATGTGCATTGA+TGG 0.354068 5:-13781416 MsG0580025093.01.T01:CDS
CTCTGAGAAGTCAAGGGACT+TGG 0.360313 5:+13780603 None:intergenic
CCAGAAAGACTCTGAATTTG+AGG 0.370802 5:+13781229 None:intergenic
CCATTCAAATGCAACACATT+AGG 0.397139 5:+13780217 None:intergenic
TAGATGAAGATGCTTCTATC+TGG 0.397277 5:-13780513 MsG0580025093.01.T01:CDS
CCTAATGTGTTGCATTTGAA+TGG 0.401214 5:-13780217 MsG0580025093.01.T01:CDS
CTTCTTCAACCATCTGCATA+AGG 0.410420 5:+13781260 None:intergenic
AAGGTGGTTCCAAAATTTGC+TGG 0.416494 5:-13780319 MsG0580025093.01.T01:CDS
AATGGATTACCAACACCAAA+AGG 0.428502 5:-13780199 MsG0580025093.01.T01:CDS
TCAACAACAATCAATTTCTC+AGG 0.429312 5:-13781179 MsG0580025093.01.T01:intron
TGAGAGTTGTTGTGGATGAT+GGG 0.430806 5:+13781661 None:intergenic
GACAAATGATTCACCAATTG+TGG 0.435186 5:-13781389 MsG0580025093.01.T01:CDS
TTCTTCAACCATCTGCATAA+GGG 0.467939 5:+13781261 None:intergenic
AGCAAAGCTTCACCAGATCC+TGG 0.479015 5:+13781301 None:intergenic
AGATGATGTGGAGGAAGAAA+TGG 0.496240 5:-13780458 MsG0580025093.01.T01:CDS
CCACCAGCAAGACCCACAAT+TGG 0.497063 5:+13781376 None:intergenic
GTGAAGAACACACCAGGATC+TGG 0.501240 5:-13781313 MsG0580025093.01.T01:CDS
GAGATTTCTCTGAGAAGTCA+AGG 0.515939 5:+13780596 None:intergenic
TATGCAGATGGTTGAAGAAG+AGG 0.527703 5:-13781257 MsG0580025093.01.T01:CDS
AGATTCTGTGTCAAAATCAA+AGG 0.534978 5:-13781449 MsG0580025093.01.T01:CDS
GATTCTGTGTCAAAATCAAA+GGG 0.549781 5:-13781448 MsG0580025093.01.T01:CDS
CTACCTCTTTGTTTCGCAGA+CGG 0.551296 5:+13781340 None:intergenic
GTGACAGAAGGAAGTGTGGT+TGG 0.552956 5:-13780547 MsG0580025093.01.T01:CDS
ATCTGGTGAAGCTTTGCTGA+GGG 0.555103 5:-13781296 MsG0580025093.01.T01:CDS
GAGAGTTGTTGTGGATGATG+GGG 0.555778 5:+13781662 None:intergenic
CAACATTAGAGTGAATGAAA+AGG 0.556085 5:-13780338 MsG0580025093.01.T01:CDS
AGATTTCTCTGAGAAGTCAA+GGG 0.560895 5:+13780597 None:intergenic
GGTGTTGGTGAGAGTTGTTG+TGG 0.563322 5:+13781653 None:intergenic
ATGGATTACCAACACCAAAA+GGG 0.569636 5:-13780198 MsG0580025093.01.T01:CDS
CCAATTGTGGGTCTTGCTGG+TGG 0.574159 5:-13781376 MsG0580025093.01.T01:CDS
TTTCATATTCACAGCTACCT+TGG 0.574412 5:+13780638 None:intergenic
GGCTTGATGAACTGTGTGAA+GGG 0.581098 5:-13780366 MsG0580025093.01.T01:CDS
TGATGAACTTGTGAATGAAG+TGG 0.585794 5:-13780266 MsG0580025093.01.T01:CDS
ACACCGTCTGCGAAACAAAG+AGG 0.595726 5:-13781343 MsG0580025093.01.T01:CDS
ACAAATGATTCACCAATTGT+GGG 0.597784 5:-13781388 MsG0580025093.01.T01:CDS
CAACAATGATGAAGATGATG+TGG 0.602585 5:-13780470 MsG0580025093.01.T01:CDS
AGTGAAGACCCTTATGCAGA+TGG 0.608040 5:-13781269 MsG0580025093.01.T01:CDS
GGGCTTGATGAACTGTGTGA+AGG 0.612048 5:-13780367 MsG0580025093.01.T01:CDS
CATTAGAGTGAATGAAAAGG+TGG 0.614379 5:-13780335 MsG0580025093.01.T01:CDS
TGCACCTCTGAGGTGACAGA+AGG 0.618247 5:-13780559 MsG0580025093.01.T01:CDS
TTACTGACTTGAAAGTGGAA+TGG 0.620132 5:+13781632 None:intergenic
ATCCTGGTGTGTTCTTCACA+CGG 0.624024 5:+13781317 None:intergenic
CAATGATGAAGATGATGTGG+AGG 0.630227 5:-13780467 MsG0580025093.01.T01:CDS
ACTTCCTTCTGTCACCTCAG+AGG 0.635704 5:+13780555 None:intergenic
TGTGGAGGAAGAAATGGCTG+AGG 0.640763 5:-13780452 MsG0580025093.01.T01:CDS
ACTTGAAAGTGGAATGGTGT+TGG 0.644715 5:+13781638 None:intergenic
GATCTGGTGAAGCTTTGCTG+AGG 0.645257 5:-13781297 MsG0580025093.01.T01:CDS
AGCCGTGTGAAGAACACACC+AGG 0.662563 5:-13781319 MsG0580025093.01.T01:CDS
ATCAGAAGAATGCACCTCTG+AGG 0.671388 5:-13780569 MsG0580025093.01.T01:CDS
TCTGGTGAAGCTTTGCTGAG+GGG 0.673835 5:-13781295 MsG0580025093.01.T01:CDS
AATTTGCTGGAAAACACACA+AGG 0.675885 5:-13780306 MsG0580025093.01.T01:CDS
TGAACTTGTGAATGAAGTGG+AGG 0.693057 5:-13780263 MsG0580025093.01.T01:CDS
TGAGGTGACAGAAGGAAGTG+TGG 0.713802 5:-13780551 MsG0580025093.01.T01:CDS
CAGGTGGTGAAAATGGACCA+AGG 0.731969 5:-13780655 MsG0580025093.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTTTCTGAAATTTT+AGG - Chr5:13780832-13780851 MsG0580025093.01.T01:intron 10.0%
!! ACTTTATAGATTATATATCT+AGG + Chr5:13780984-13781003 None:intergenic 15.0%
!!! ATTTTTTTCAAGATGTTTAT+TGG + Chr5:13780269-13780288 None:intergenic 15.0%
!!! CATGCAAATTTATATTTTTA+TGG - Chr5:13780225-13780244 MsG0580025093.01.T01:CDS 15.0%
!!! TGTTTTTTTTTTTTTTTTGC+AGG - Chr5:13780335-13780354 MsG0580025093.01.T01:CDS 15.0%
!!! TTATTTCATGAATTTTTCTT+TGG + Chr5:13780682-13780701 None:intergenic 15.0%
!! AAAAAGAAGAAGATTATGAA+GGG - Chr5:13781429-13781448 MsG0580025093.01.T01:CDS 20.0%
!! AAACATCTTGAAAAAAATTC+TGG - Chr5:13780271-13780290 MsG0580025093.01.T01:CDS 20.0%
!! AACATCTTGAAAAAAATTCT+GGG - Chr5:13780272-13780291 MsG0580025093.01.T01:CDS 20.0%
!! ATAAGATCTCAATTTAATGA+TGG - Chr5:13781138-13781157 MsG0580025093.01.T01:intron 20.0%
!!! TCAATTGCTTTTGAAAAAAA+TGG - Chr5:13780950-13780969 MsG0580025093.01.T01:intron 20.0%
!!! TTTTGAAAAAAATGGAGATT+TGG - Chr5:13780958-13780977 MsG0580025093.01.T01:intron 20.0%
! AACATAAAGATTTCAACTAC+TGG + Chr5:13780810-13780829 None:intergenic 25.0%
! ACATGTGAGTTTCAATAAAA+TGG + Chr5:13780778-13780797 None:intergenic 25.0%
! CACTTAATGAACTATTTATG+TGG - Chr5:13781016-13781035 MsG0580025093.01.T01:intron 25.0%
! CATAAATAGTTCATTAAGTG+AGG + Chr5:13781016-13781035 None:intergenic 25.0%
! CATGTGAGTTTCAATAAAAT+GGG + Chr5:13780777-13780796 None:intergenic 25.0%
! GAAAAAGAAGAAGATTATGA+AGG - Chr5:13781428-13781447 MsG0580025093.01.T01:CDS 25.0%
!! CATGAATTTTTCTTTGGTTA+GGG + Chr5:13780676-13780695 None:intergenic 25.0%
!! TCATGAATTTTTCTTTGGTT+AGG + Chr5:13780677-13780696 None:intergenic 25.0%
!!! GCAAGTCTTTTTAATTAGTT+TGG - Chr5:13780750-13780769 MsG0580025093.01.T01:intron 25.0%
!!! TTTTTTTACTGACTTGAAAG+TGG + Chr5:13780192-13780211 None:intergenic 25.0%
ACAAATGATTCACCAATTGT+GGG - Chr5:13780428-13780447 MsG0580025093.01.T01:CDS 30.0%
AGATTCTGTGTCAAAATCAA+AGG - Chr5:13780367-13780386 MsG0580025093.01.T01:CDS 30.0%
CAACATTAGAGTGAATGAAA+AGG - Chr5:13781478-13781497 MsG0580025093.01.T01:intron 30.0%
GATTCTGTGTCAAAATCAAA+GGG - Chr5:13780368-13780387 MsG0580025093.01.T01:CDS 30.0%
TCAACAACAATCAATTTCTC+AGG - Chr5:13780637-13780656 MsG0580025093.01.T01:CDS 30.0%
! AGTGATTGTATAAAGTGTCT+TGG - Chr5:13781078-13781097 MsG0580025093.01.T01:intron 30.0%
CAACAATGATGAAGATGATG+TGG - Chr5:13781346-13781365 MsG0580025093.01.T01:CDS 35.0%
CATTAGAGTGAATGAAAAGG+TGG - Chr5:13781481-13781500 MsG0580025093.01.T01:intron 35.0%
CCATTCAAATGCAACACATT+AGG + Chr5:13781602-13781621 None:intergenic 35.0%
CCTAATGTGTTGCATTTGAA+TGG - Chr5:13781599-13781618 MsG0580025093.01.T01:intron 35.0%
GACAAATGATTCACCAATTG+TGG - Chr5:13780427-13780446 MsG0580025093.01.T01:CDS 35.0%
GATCTCAATTTAATGATGGC+AGG - Chr5:13781142-13781161 MsG0580025093.01.T01:intron 35.0%
TAGATGAAGATGCTTCTATC+TGG - Chr5:13781303-13781322 MsG0580025093.01.T01:CDS 35.0%
TGATGAACTTGTGAATGAAG+TGG - Chr5:13781550-13781569 MsG0580025093.01.T01:intron 35.0%
TTCTTCAACCATCTGCATAA+GGG + Chr5:13780558-13780577 None:intergenic 35.0%
TTTCATATTCACAGCTACCT+TGG + Chr5:13781181-13781200 None:intergenic 35.0%
! AAACGAAGATGTTTCCCTTT+TGG + Chr5:13781635-13781654 None:intergenic 35.0%
! AGATTTCTCTGAGAAGTCAA+GGG + Chr5:13781222-13781241 None:intergenic 35.0%
! ATGGATTACCAACACCAAAA+GGG - Chr5:13781618-13781637 MsG0580025093.01.T01:intron 35.0%
! GTGTGTTTTCCAGCAAATTT+TGG + Chr5:13781509-13781528 None:intergenic 35.0%
! TGTCTTGGTAGCATAATTTG+TGG - Chr5:13781093-13781112 MsG0580025093.01.T01:intron 35.0%
!! AATGGATTACCAACACCAAA+AGG - Chr5:13781617-13781636 MsG0580025093.01.T01:intron 35.0%
!! AATTTGCTGGAAAACACACA+AGG - Chr5:13781510-13781529 MsG0580025093.01.T01:intron 35.0%
!! GAAACATCTTCGTTTTTCTG+AGG - Chr5:13781640-13781659 MsG0580025093.01.T01:CDS 35.0%
!! TTACTGACTTGAAAGTGGAA+TGG + Chr5:13780187-13780206 None:intergenic 35.0%
AAGGTGGTTCCAAAATTTGC+TGG - Chr5:13781497-13781516 MsG0580025093.01.T01:intron 40.0%
ACTTGAAAGTGGAATGGTGT+TGG + Chr5:13780181-13780200 None:intergenic 40.0%
AGATGATGTGGAGGAAGAAA+TGG - Chr5:13781358-13781377 MsG0580025093.01.T01:CDS 40.0%
CAATGATGAAGATGATGTGG+AGG - Chr5:13781349-13781368 MsG0580025093.01.T01:CDS 40.0%
CCAGAAAGACTCTGAATTTG+AGG + Chr5:13780590-13780609 None:intergenic 40.0%
CCTCAAATTCAGAGTCTTTC+TGG - Chr5:13780587-13780606 MsG0580025093.01.T01:CDS 40.0%
CTCAATTTAATGATGGCAGG+TGG - Chr5:13781145-13781164 MsG0580025093.01.T01:intron 40.0%
CTTCTTCAACCATCTGCATA+AGG + Chr5:13780559-13780578 None:intergenic 40.0%
TATGCAGATGGTTGAAGAAG+AGG - Chr5:13780559-13780578 MsG0580025093.01.T01:CDS 40.0%
TCAAGATCGATGTGCATTGA+TGG - Chr5:13780400-13780419 MsG0580025093.01.T01:CDS 40.0%
TGAACTTGTGAATGAAGTGG+AGG - Chr5:13781553-13781572 MsG0580025093.01.T01:intron 40.0%
TGAGAGTTGTTGTGGATGAT+GGG + Chr5:13780158-13780177 None:intergenic 40.0%
! GAGATTTCTCTGAGAAGTCA+AGG + Chr5:13781223-13781242 None:intergenic 40.0%
!! ACATCTTCGTTTTTCTGAGG+AGG - Chr5:13781643-13781662 MsG0580025093.01.T01:CDS 40.0%
!! AGATGTTTCCCTTTTGGTGT+TGG + Chr5:13781629-13781648 None:intergenic 40.0%
AGTGAAGACCCTTATGCAGA+TGG - Chr5:13780547-13780566 MsG0580025093.01.T01:CDS 45.0%
ATCAGAAGAATGCACCTCTG+AGG - Chr5:13781247-13781266 MsG0580025093.01.T01:CDS 45.0%
ATCCTGGTGTGTTCTTCACA+CGG + Chr5:13780502-13780521 None:intergenic 45.0%
ATCTGGTGAAGCTTTGCTGA+GGG - Chr5:13780520-13780539 MsG0580025093.01.T01:CDS 45.0%
ATGATGGCAGGTGGTGAAAA+TGG - Chr5:13781154-13781173 MsG0580025093.01.T01:intron 45.0%
GAGAGTTGTTGTGGATGATG+GGG + Chr5:13780157-13780176 None:intergenic 45.0%
GGCTTGATGAACTGTGTGAA+GGG - Chr5:13781450-13781469 MsG0580025093.01.T01:CDS 45.0%
GTGAGAGTTGTTGTGGATGA+TGG + Chr5:13780159-13780178 None:intergenic 45.0%
! CTACCTCTTTGTTTCGCAGA+CGG + Chr5:13780479-13780498 None:intergenic 45.0%
!! TCGTTTTTCTGAGGAGGAAG+AGG - Chr5:13781649-13781668 MsG0580025093.01.T01:CDS 45.0%
!! TTTTCTGAGGAGGAAGAGGA+AGG - Chr5:13781653-13781672 MsG0580025093.01.T01:CDS 45.0%
ACACCGTCTGCGAAACAAAG+AGG - Chr5:13780473-13780492 MsG0580025093.01.T01:CDS 50.0%
ACTTCCTTCTGTCACCTCAG+AGG + Chr5:13781264-13781283 None:intergenic 50.0%
AGCAAAGCTTCACCAGATCC+TGG + Chr5:13780518-13780537 None:intergenic 50.0%
CAGGTGGTGAAAATGGACCA+AGG - Chr5:13781161-13781180 MsG0580025093.01.T01:intron 50.0%
GATCTGGTGAAGCTTTGCTG+AGG - Chr5:13780519-13780538 MsG0580025093.01.T01:CDS 50.0%
GGGCTTGATGAACTGTGTGA+AGG - Chr5:13781449-13781468 MsG0580025093.01.T01:CDS 50.0%
GTGAAGAACACACCAGGATC+TGG - Chr5:13780503-13780522 MsG0580025093.01.T01:CDS 50.0%
TCACCAATTGTGGGTCTTGC+TGG - Chr5:13780437-13780456 MsG0580025093.01.T01:CDS 50.0%
TCTGGTGAAGCTTTGCTGAG+GGG - Chr5:13780521-13780540 MsG0580025093.01.T01:CDS 50.0%
TGAGGTGACAGAAGGAAGTG+TGG - Chr5:13781265-13781284 MsG0580025093.01.T01:CDS 50.0%
TGTGGAGGAAGAAATGGCTG+AGG - Chr5:13781364-13781383 MsG0580025093.01.T01:CDS 50.0%
! CTCTGAGAAGTCAAGGGACT+TGG + Chr5:13781216-13781235 None:intergenic 50.0%
! GTGACAGAAGGAAGTGTGGT+TGG - Chr5:13781269-13781288 MsG0580025093.01.T01:CDS 50.0%
!! GGTGTTGGTGAGAGTTGTTG+TGG + Chr5:13780166-13780185 None:intergenic 50.0%
AGCCGTGTGAAGAACACACC+AGG - Chr5:13780497-13780516 MsG0580025093.01.T01:CDS 55.0%
CCAATTGTGGGTCTTGCTGG+TGG - Chr5:13780440-13780459 MsG0580025093.01.T01:CDS 55.0%
CCACCAGCAAGACCCACAAT+TGG + Chr5:13780443-13780462 None:intergenic 55.0%
!! TGCACCTCTGAGGTGACAGA+AGG - Chr5:13781257-13781276 MsG0580025093.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 13780147 13781691 13780147 ID=MsG0580025093.01;Name=MsG0580025093.01
Chr5 mRNA 13780147 13781691 13780147 ID=MsG0580025093.01.T01;Parent=MsG0580025093.01;Name=MsG0580025093.01.T01;_AED=0.28;_eAED=0.29;_QI=0|0|0|1|0.5|0.66|3|0|293
Chr5 exon 13781640 13781691 13781640 ID=MsG0580025093.01.T01:exon:20452;Parent=MsG0580025093.01.T01
Chr5 exon 13781180 13781481 13781180 ID=MsG0580025093.01.T01:exon:20451;Parent=MsG0580025093.01.T01
Chr5 exon 13780147 13780674 13780147 ID=MsG0580025093.01.T01:exon:20450;Parent=MsG0580025093.01.T01
Chr5 CDS 13781640 13781691 13781640 ID=MsG0580025093.01.T01:cds;Parent=MsG0580025093.01.T01
Chr5 CDS 13781180 13781481 13781180 ID=MsG0580025093.01.T01:cds;Parent=MsG0580025093.01.T01
Chr5 CDS 13780147 13780674 13780147 ID=MsG0580025093.01.T01:cds;Parent=MsG0580025093.01.T01
Gene Sequence

>MsG0580025093.01.T01

ATGGAAACCCCATCATCCACAACAACTCTCACCAACACCATTCCACTTTCAAGAAAATTTGAAGATTCTGTGTCAAAATCAAAGGGTCAACAAAATCAAGATCGATGTGCATTGATGGACATGACAAATGATTCACCAATTGTGGGTCTTGCTGGTGGAAATCTCGAGACACCGTCTGCGAAACAAAGAGGTAGCCGTGTGAAGAACACACCAGGATCTGGTGAAGCTTTGCTGAGGGGACAAGTGAAGACCCTTATGCAGATGGTTGAAGAAGAGGCTTCTCCTCAAATTCAGAGTCTTTCTGGTAGTGTCAATAACAATGCTTCTGTTGTTCAACAACAATCAATTTCTCAGGTGGTGAAAATGGACCAAGGTAGCTGTGAATATGAAAAGTGTGAGATAACCAAGTCCCTTGACTTCTCAGAGAAATCTCAAGTATCAGAAGAATGCACCTCTGAGGTGACAGAAGGAAGTGTGGTTGGTAGCTGCAGCATAGATGAAGATGCTTCTATCTGGTCAATGCAAGTCAACTCATGCAACAATGATGAAGATGATGTGGAGGAAGAAATGGCTGAGGAAGATGAAGAAGAAGAAGAAGAAGAAGACTACTATGATGTTGAAAAAGAAGAAGATTATGAAGGGCTTGATGAACTGTGTGAAGGGCTAAACAACATTAGAGTGAATGAAAAGGTGGTTCCAAAATTTGCTGGAAAACACACAAGGTTTGTTTACAACAGTGATGATGAACTTGTGAATGAAGTGGAGGATGAAAGTGTGAATGCTGATTCACCTAATGTGTTGCATTTGAATGGATTACCAACACCAAAAGGGAAACATCTTCGTTTTTCTGAGGAGGAAGAGGAAGGAAAATCTGCTAAGTGA

Protein sequence

>MsG0580025093.01.T01

METPSSTTTLTNTIPLSRKFEDSVSKSKGQQNQDRCALMDMTNDSPIVGLAGGNLETPSAKQRGSRVKNTPGSGEALLRGQVKTLMQMVEEEASPQIQSLSGSVNNNASVVQQQSISQVVKMDQGSCEYEKCEITKSLDFSEKSQVSEECTSEVTEGSVVGSCSIDEDASIWSMQVNSCNNDEDDVEEEMAEEDEEEEEEEDYYDVEKEEDYEGLDELCEGLNNIRVNEKVVPKFAGKHTRFVYNSDDELVNEVEDESVNADSPNVLHLNGLPTPKGKHLRFSEEEEEGKSAK*