Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000109.01.T01 | XP_003589348.2 | 98.182 | 55 | 1 | 0 | 1 | 55 | 282 | 336 | 2.81E-33 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000109.01.T01 | P25804 | 92.727 | 55 | 4 | 0 | 1 | 55 | 279 | 333 | 1.27E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000109.01.T01 | A0A396JH07 | 98.182 | 55 | 1 | 0 | 1 | 55 | 282 | 336 | 1.34e-33 | 129 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000109.01 | MsG0180000110.01 | 0.906175 | 1.963216e-80 | 3.285935e-76 |
| MsG0180000109.01 | MsG0380017793.01 | 0.801037 | 1.087516e-48 | 5.141621e-46 |
| MsG0180000109.01 | MsG0680031795.01 | -0.817095 | 3.938058e-52 | 2.841049e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000109.01.T01 | MTR_1g023210 | 96.364 | 55 | 2 | 0 | 1 | 55 | 282 | 336 | 1.06e-36 | 127 |
| MsG0180000109.01.T01 | MTR_4g071060 | 83.636 | 55 | 8 | 1 | 1 | 55 | 279 | 332 | 6.03e-30 | 110 |
| MsG0180000109.01.T01 | MTR_1g094135 | 67.273 | 55 | 18 | 0 | 1 | 55 | 288 | 342 | 1.16e-21 | 88.2 |
| MsG0180000109.01.T01 | MTR_4g072340 | 66.667 | 57 | 18 | 1 | 1 | 57 | 247 | 302 | 1.92e-21 | 87.0 |
| MsG0180000109.01.T01 | MTR_4g073660 | 66.667 | 57 | 18 | 1 | 1 | 57 | 246 | 301 | 1.98e-21 | 87.0 |
| MsG0180000109.01.T01 | MTR_4g072790 | 66.667 | 57 | 14 | 2 | 1 | 57 | 247 | 298 | 2.27e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000109.01.T01 | AT4G16190 | 69.565 | 69 | 18 | 2 | 1 | 67 | 288 | 355 | 1.48e-28 | 106 |
| MsG0180000109.01.T01 | AT4G39090 | 76.364 | 55 | 12 | 1 | 1 | 55 | 283 | 336 | 1.16e-27 | 103 |
| MsG0180000109.01.T01 | AT2G21430 | 74.545 | 55 | 13 | 1 | 1 | 55 | 280 | 333 | 3.08e-26 | 100 |
| MsG0180000109.01.T01 | AT3G54940 | 62.903 | 62 | 23 | 0 | 1 | 62 | 284 | 345 | 4.71e-24 | 94.4 |
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCACGATCATGTGTATTTA+TGG | 0.248013 | 1:-1589518 | MsG0180000109.01.T01:CDS |
| AATTGGGGTCCTTCTGATTC+TGG | 0.263837 | 1:-1589571 | MsG0180000109.01.T01:CDS |
| AATCAGAAGGACCCCAATTC+TGG | 0.285746 | 1:+1589575 | None:intergenic |
| GATTGAAGGAAAAGCCTTAC+TGG | 0.291151 | 1:-1589618 | MsG0180000109.01.T01:CDS |
| AGAATTCATGGGGCCAGAAT+TGG | 0.303276 | 1:-1589588 | MsG0180000109.01.T01:CDS |
| AACTATTTCCGCATAGTATC+AGG | 0.339374 | 1:-1589546 | MsG0180000109.01.T01:CDS |
| GATCATGGGGTTCTTCTAGT+TGG | 0.348875 | 1:-1589670 | MsG0180000109.01.T01:CDS |
| GGGGTTCTTCTAGTTGGTTT+TGG | 0.352074 | 1:-1589664 | MsG0180000109.01.T01:CDS |
| GAATTCATGGGGCCAGAATT+GGG | 0.380855 | 1:-1589587 | MsG0180000109.01.T01:CDS |
| CATCTGTGCCAAAGCACGTT+TGG | 0.391375 | 1:-1589692 | MsG0180000109.01.T01:CDS |
| ACTGGATCATAAAGAATTCA+TGG | 0.401274 | 1:-1589600 | MsG0180000109.01.T01:CDS |
| CGTGCTTTGGCACAGATGTA+TGG | 0.406234 | 1:+1589697 | None:intergenic |
| CCCATGATCCAAACGTGCTT+TGG | 0.406480 | 1:+1589684 | None:intergenic |
| CCAAAGCACGTTTGGATCAT+GGG | 0.425598 | 1:-1589684 | MsG0180000109.01.T01:CDS |
| CTATGCTCCCATTCGATTGA+AGG | 0.426934 | 1:-1589632 | MsG0180000109.01.T01:CDS |
| GCCAAAGCACGTTTGGATCA+TGG | 0.451166 | 1:-1589685 | MsG0180000109.01.T01:CDS |
| AACGTGGCAGTTACATGAAT+GGG | 0.469525 | 1:-1589437 | MsG0180000109.01.T01:CDS |
| GAATTCTTTATGATCCAGTA+AGG | 0.473251 | 1:+1589604 | None:intergenic |
| CAACGTGGCAGTTACATGAA+TGG | 0.506698 | 1:-1589438 | MsG0180000109.01.T01:CDS |
| CTGGATCATAAAGAATTCAT+GGG | 0.512632 | 1:-1589599 | MsG0180000109.01.T01:CDS |
| ATGTAACTGCCACGTTGGAA+TGG | 0.542764 | 1:+1589444 | None:intergenic |
| GTGCTTTGGCACAGATGTAT+GGG | 0.545478 | 1:+1589698 | None:intergenic |
| AATTCATGGGGCCAGAATTG+GGG | 0.572044 | 1:-1589586 | MsG0180000109.01.T01:CDS |
| CATTCATGTAACTGCCACGT+TGG | 0.586640 | 1:+1589439 | None:intergenic |
| TCATGTGTATTTATGGAGGT+CGG | 0.600155 | 1:-1589511 | MsG0180000109.01.T01:CDS |
| AATGGCTAAATAAACATCAC+AGG | 0.607102 | 1:+1589462 | None:intergenic |
| CAAAGCACGTTTGGATCATG+GGG | 0.618631 | 1:-1589683 | MsG0180000109.01.T01:CDS |
| AATAGTTTGCCAGAATCAGA+AGG | 0.625402 | 1:+1589562 | None:intergenic |
| ACGATCATGTGTATTTATGG+AGG | 0.657314 | 1:-1589515 | MsG0180000109.01.T01:CDS |
| TCCATAAATACACATGATCG+TGG | 0.673361 | 1:+1589517 | None:intergenic |
| GGCAGTTTCCTGATACTATG+CGG | 0.687341 | 1:+1589538 | None:intergenic |
| TGGATCATAAAGAATTCATG+GGG | 0.698117 | 1:-1589598 | MsG0180000109.01.T01:CDS |
| TTTATTTAGCCATTCCAACG+TGG | 0.730224 | 1:-1589453 | MsG0180000109.01.T01:CDS |
| TGGATGCAGACATATATGAG+TGG | 0.766052 | 1:-1589730 | MsG0180000109.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGGCTAAATAAACATCAC+AGG | + | Chr1:1589679-1589698 | None:intergenic | 30.0% | |
| ACTGGATCATAAAGAATTCA+TGG | - | Chr1:1589538-1589557 | MsG0180000109.01.T01:CDS | 30.0% | |
| CTGGATCATAAAGAATTCAT+GGG | - | Chr1:1589539-1589558 | MsG0180000109.01.T01:CDS | 30.0% | |
| GAATTCTTTATGATCCAGTA+AGG | + | Chr1:1589537-1589556 | None:intergenic | 30.0% | |
| TGGATCATAAAGAATTCATG+GGG | - | Chr1:1589540-1589559 | MsG0180000109.01.T01:CDS | 30.0% | |
| AACTATTTCCGCATAGTATC+AGG | - | Chr1:1589592-1589611 | MsG0180000109.01.T01:CDS | 35.0% | |
| ACGATCATGTGTATTTATGG+AGG | - | Chr1:1589623-1589642 | MsG0180000109.01.T01:CDS | 35.0% | |
| TCATGTGTATTTATGGAGGT+CGG | - | Chr1:1589627-1589646 | MsG0180000109.01.T01:CDS | 35.0% | |
| TCCATAAATACACATGATCG+TGG | + | Chr1:1589624-1589643 | None:intergenic | 35.0% | |
| TTTATTTAGCCATTCCAACG+TGG | - | Chr1:1589685-1589704 | MsG0180000109.01.T01:CDS | 35.0% | |
| ! | AATAGTTTGCCAGAATCAGA+AGG | + | Chr1:1589579-1589598 | None:intergenic | 35.0% |
| AACGTGGCAGTTACATGAAT+GGG | - | Chr1:1589701-1589720 | MsG0180000109.01.T01:CDS | 40.0% | |
| GATTGAAGGAAAAGCCTTAC+TGG | - | Chr1:1589520-1589539 | MsG0180000109.01.T01:CDS | 40.0% | |
| GCCACGATCATGTGTATTTA+TGG | - | Chr1:1589620-1589639 | MsG0180000109.01.T01:CDS | 40.0% | |
| ! | AGGCTTTTCCTTCAATCGAA+TGG | + | Chr1:1589517-1589536 | None:intergenic | 40.0% |
| ! | GGCTTTTCCTTCAATCGAAT+GGG | + | Chr1:1589516-1589535 | None:intergenic | 40.0% |
| AATCAGAAGGACCCCAATTC+TGG | + | Chr1:1589566-1589585 | None:intergenic | 45.0% | |
| AATTCATGGGGCCAGAATTG+GGG | - | Chr1:1589552-1589571 | MsG0180000109.01.T01:CDS | 45.0% | |
| AATTGGGGTCCTTCTGATTC+TGG | - | Chr1:1589567-1589586 | MsG0180000109.01.T01:CDS | 45.0% | |
| AGAATTCATGGGGCCAGAAT+TGG | - | Chr1:1589550-1589569 | MsG0180000109.01.T01:CDS | 45.0% | |
| ATGTAACTGCCACGTTGGAA+TGG | + | Chr1:1589697-1589716 | None:intergenic | 45.0% | |
| CAAAGCACGTTTGGATCATG+GGG | - | Chr1:1589455-1589474 | MsG0180000109.01.T01:CDS | 45.0% | |
| CAACGTGGCAGTTACATGAA+TGG | - | Chr1:1589700-1589719 | MsG0180000109.01.T01:CDS | 45.0% | |
| CATTCATGTAACTGCCACGT+TGG | + | Chr1:1589702-1589721 | None:intergenic | 45.0% | |
| CCAAAGCACGTTTGGATCAT+GGG | - | Chr1:1589454-1589473 | MsG0180000109.01.T01:CDS | 45.0% | |
| CTATGCTCCCATTCGATTGA+AGG | - | Chr1:1589506-1589525 | MsG0180000109.01.T01:CDS | 45.0% | |
| GAATTCATGGGGCCAGAATT+GGG | - | Chr1:1589551-1589570 | MsG0180000109.01.T01:CDS | 45.0% | |
| GATCATGGGGTTCTTCTAGT+TGG | - | Chr1:1589468-1589487 | MsG0180000109.01.T01:CDS | 45.0% | |
| GGCAGTTTCCTGATACTATG+CGG | + | Chr1:1589603-1589622 | None:intergenic | 45.0% | |
| GTGCTTTGGCACAGATGTAT+GGG | + | Chr1:1589443-1589462 | None:intergenic | 45.0% | |
| !! | GGGGTTCTTCTAGTTGGTTT+TGG | - | Chr1:1589474-1589493 | MsG0180000109.01.T01:CDS | 45.0% |
| !!! | CTTCTAGTTGGTTTTGGCAG+TGG | - | Chr1:1589480-1589499 | MsG0180000109.01.T01:CDS | 45.0% |
| CATCTGTGCCAAAGCACGTT+TGG | - | Chr1:1589446-1589465 | MsG0180000109.01.T01:CDS | 50.0% | |
| CCCATGATCCAAACGTGCTT+TGG | + | Chr1:1589457-1589476 | None:intergenic | 50.0% | |
| CGTGCTTTGGCACAGATGTA+TGG | + | Chr1:1589444-1589463 | None:intergenic | 50.0% | |
| GCCAAAGCACGTTTGGATCA+TGG | - | Chr1:1589453-1589472 | MsG0180000109.01.T01:CDS | 50.0% | |
| !!! | CTAGTTGGTTTTGGCAGTGG+TGG | - | Chr1:1589483-1589502 | MsG0180000109.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 1589411 | 1589749 | 1589411 | ID=MsG0180000109.01;Name=MsG0180000109.01 |
| Chr1 | mRNA | 1589411 | 1589749 | 1589411 | ID=MsG0180000109.01.T01;Parent=MsG0180000109.01;Name=MsG0180000109.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|112 |
| Chr1 | exon | 1589411 | 1589749 | 1589411 | ID=MsG0180000109.01.T01:exon:7220;Parent=MsG0180000109.01.T01 |
| Chr1 | CDS | 1589411 | 1589749 | 1589411 | ID=MsG0180000109.01.T01:cds;Parent=MsG0180000109.01.T01 |
| Gene Sequence |
| Protein sequence |
