Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000154.01.T01 | XP_013466098.1 | 97.194 | 499 | 14 | 0 | 1 | 499 | 1 | 499 | 0 | 1009 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000154.01.T01 | A0A072VE89 | 97.194 | 499 | 14 | 0 | 1 | 499 | 1 | 499 | 0.0 | 1009 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047797.01 | MsG0180000154.01 | 0.808085 | 3.682421e-50 | 2.086066e-47 |
| MsG0080047869.01 | MsG0180000154.01 | 0.802245 | 6.147921e-49 | 2.996834e-46 |
| MsG0080048461.01 | MsG0180000154.01 | 0.824803 | 6.622847e-54 | 5.928782e-51 |
| MsG0180000065.01 | MsG0180000154.01 | 0.820815 | 5.618438e-53 | 4.492452e-50 |
| MsG0180000091.01 | MsG0180000154.01 | 0.802824 | 4.670140e-49 | 2.310457e-46 |
| MsG0180000123.01 | MsG0180000154.01 | 0.806255 | 8.991143e-50 | 4.857547e-47 |
| MsG0180000154.01 | MsG0180000470.01 | 0.819525 | 1.109440e-52 | 8.558565e-50 |
| MsG0180000154.01 | MsG0180000555.01 | 0.840904 | 6.565749e-58 | 9.488553e-55 |
| MsG0180000154.01 | MsG0180000585.01 | 0.808470 | 3.049194e-50 | 1.744974e-47 |
| MsG0180000154.01 | MsG0180000702.01 | 0.809563 | 1.778586e-50 | 1.047761e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000154.01.T01 | MTR_1g022245 | 97.194 | 499 | 14 | 0 | 1 | 499 | 1 | 499 | 0.0 | 1009 |
| MsG0180000154.01.T01 | MTR_4g107370 | 53.484 | 488 | 219 | 4 | 1 | 483 | 6 | 490 | 0.0 | 539 |
| MsG0180000154.01.T01 | MTR_2g030420 | 54.082 | 490 | 178 | 5 | 3 | 477 | 8 | 465 | 0.0 | 539 |
| MsG0180000154.01.T01 | MTR_1g022250 | 55.556 | 477 | 194 | 5 | 3 | 476 | 5 | 466 | 0.0 | 536 |
| MsG0180000154.01.T01 | MTR_6g009540 | 51.810 | 525 | 217 | 9 | 3 | 511 | 5 | 509 | 2.74e-180 | 516 |
| MsG0180000154.01.T01 | MTR_7g008040 | 53.017 | 464 | 209 | 4 | 1 | 460 | 1 | 459 | 1.06e-175 | 506 |
| MsG0180000154.01.T01 | MTR_6g009520 | 48.462 | 520 | 247 | 6 | 3 | 511 | 7 | 516 | 5.77e-169 | 488 |
| MsG0180000154.01.T01 | MTR_6g009553 | 53.319 | 452 | 194 | 7 | 21 | 460 | 1 | 447 | 3.23e-165 | 476 |
| MsG0180000154.01.T01 | MTR_6g009720 | 51.183 | 465 | 217 | 4 | 3 | 460 | 7 | 468 | 2.07e-164 | 476 |
| MsG0180000154.01.T01 | MTR_6g009770 | 50.644 | 466 | 196 | 8 | 3 | 460 | 7 | 446 | 1.63e-154 | 450 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000154.01.T01 | AT3G28510 | 50.855 | 468 | 216 | 4 | 4 | 467 | 7 | 464 | 9.08e-167 | 483 |
| MsG0180000154.01.T01 | AT3G28580 | 50.433 | 462 | 214 | 6 | 1 | 457 | 4 | 455 | 6.61e-158 | 459 |
| MsG0180000154.01.T01 | AT3G28540 | 49.679 | 467 | 215 | 7 | 1 | 459 | 1 | 455 | 4.76e-156 | 454 |
| MsG0180000154.01.T01 | AT3G28540 | 49.679 | 467 | 215 | 7 | 1 | 459 | 1 | 455 | 5.42e-156 | 454 |
| MsG0180000154.01.T01 | AT5G40010 | 48.077 | 520 | 246 | 7 | 1 | 511 | 4 | 508 | 1.27e-152 | 446 |
| MsG0180000154.01.T01 | AT3G28600 | 49.895 | 475 | 190 | 10 | 8 | 465 | 7 | 450 | 5.62e-150 | 437 |
| MsG0180000154.01.T01 | AT3G28610 | 48.434 | 479 | 194 | 10 | 7 | 465 | 5 | 450 | 2.54e-148 | 433 |
| MsG0180000154.01.T01 | AT5G40000 | 46.720 | 503 | 205 | 10 | 1 | 481 | 4 | 465 | 1.54e-145 | 426 |
| MsG0180000154.01.T01 | AT3G28580 | 47.186 | 462 | 207 | 7 | 1 | 457 | 4 | 433 | 1.34e-140 | 414 |
| MsG0180000154.01.T01 | AT3G28570 | 46.680 | 482 | 202 | 9 | 8 | 476 | 9 | 448 | 4.98e-138 | 406 |
Find 82 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGTATTATGCGAAAAT+TGG | 0.315513 | 1:-2145269 | MsG0180000154.01.T01:CDS |
| AAAGTCTTTAGTTCTAAAGA+TGG | 0.468242 | 1:-2145669 | MsG0180000154.01.T01:CDS |
| AACAAAAGGGTAACAAAAGT+TGG | 0.459264 | 1:+2145817 | None:intergenic |
| AACCAAACATGTTTGAACTT+TGG | 0.318708 | 1:-2145949 | None:intergenic |
| AACCAAGCAAAGAAACTTCA+AGG | 0.474512 | 1:-2145704 | MsG0180000154.01.T01:CDS |
| AAGAATCTAACCTCAACAAA+AGG | 0.394204 | 1:+2145803 | None:intergenic |
| AAGAGAGGCTATTTGCTTTA+TGG | 0.209267 | 1:-2145236 | MsG0180000154.01.T01:CDS |
| AAGCTTTACACAAATTGCAC+AGG | 0.572705 | 1:-2145425 | MsG0180000154.01.T01:CDS |
| AATATGAAAGGGGAGGTAAG+TGG | 0.596088 | 1:-2145394 | MsG0180000154.01.T01:CDS |
| AATCACGAGTAAATCTGTTG+TGG | 0.629066 | 1:-2145090 | MsG0180000154.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAAAGGGTAACAAAAGT+TGG | + | Chr1:2144552-2144571 | None:intergenic | 30.0% | |
| AAGAATCTAACCTCAACAAA+AGG | + | Chr1:2144566-2144585 | None:intergenic | 30.0% | |
| AGAAAAAGAGGACAAAAATG+AGG | - | Chr1:2145366-2145385 | MsG0180000154.01.T01:CDS | 30.0% | |
| AGAATCTAACCTCAACAAAA+GGG | + | Chr1:2144565-2144584 | None:intergenic | 30.0% | |
| GGGTTATTGAATTTCATTGA+TGG | - | Chr1:2145475-2145494 | MsG0180000154.01.T01:CDS | 30.0% | |
| TGAATTTCATTGATGGGATT+TGG | - | Chr1:2145482-2145501 | MsG0180000154.01.T01:CDS | 30.0% | |
| TGATCTTGTTACATTCAGTA+AGG | - | Chr1:2145072-2145091 | MsG0180000154.01.T01:CDS | 30.0% | |
| AAAAGAGGACAAAAATGAGG+AGG | - | Chr1:2145369-2145388 | MsG0180000154.01.T01:CDS | 35.0% | |
| AACCAAGCAAAGAAACTTCA+AGG | - | Chr1:2144662-2144681 | MsG0180000154.01.T01:CDS | 35.0% | |
| AAGAGAGGCTATTTGCTTTA+TGG | - | Chr1:2145130-2145149 | MsG0180000154.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 2144425 | 2145963 | 2144425 | ID=MsG0180000154.01;Name=MsG0180000154.01 |
| Chr1 | mRNA | 2144425 | 2145963 | 2144425 | ID=MsG0180000154.01.T01;Parent=MsG0180000154.01;Name=MsG0180000154.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|512 |
| Chr1 | exon | 2144425 | 2145963 | 2144425 | ID=MsG0180000154.01.T01:exon:6760;Parent=MsG0180000154.01.T01 |
| Chr1 | CDS | 2144425 | 2145963 | 2144425 | ID=MsG0180000154.01.T01:cds;Parent=MsG0180000154.01.T01 |
| Gene Sequence |
>MsG0180000154.01.T01 ATGTTTGAACTTTGGGGTCTAATAGGTTCAAAGCTTGCAAGCTTAATGTTTATATGGGCTATGATTCAAAGTTATTGTCCTCATCAAGTTCATGCACTCATTGAGAAATTCTCAGAGAAATTAGCCAACTTTTGTTACCCTTTTGTTGAGGTTAGATTCTTTGAGAACATTGGTGATTACATGAAAACCAATGAAGCTTTCTTATACATTGAAAACTACTTAACATCTAAATCAACCAACCAAGCAAAGAAACTTCAAGGTGAATATGCGAGAAAGTCTTTAGTTCTAAAGATGGATGAAAGACAAAAGTTTTATGATGAGTTTGAAGGGGTTAAGTTTGTTTGGAGTCTAATCAAGATTGTCCCTAACACAAATTCTGTTTCATTCTATCCAGCTTCAGACAAAAGATTCTACCTTCTCACATTTCATCGCAGTCATCGCGATTTTGTTGAGAAATCTTACCTTAACCATGTTTTGGAACAAGGTAAGGAGATTGGTTTGAGTAAGAGGCAGAGAAAGCTTTACACAAATTGCACAGGTAATGGTGAATATGAAAGGGGAGGTAAGTGGAGTCATGTGATATTTGAACATCCATCTTCTTTTGAGACAATTGCAATGAATCCTAAAAAGAAGAAAGAGATTGTTGATGATCTTGTTACATTCAGTAAGGCTAAAGAGTATTATGCGAAAATTGGTAAGCCTTGGAAGAGAGGCTATTTGCTTTATGGTCCTCCTGGAACAGGAAAATCATCACTAGTTGCTGCTATTGCTAATTTTTTGAAGTATGATATTTATGACATTGAGTTAACCAATGTGAAAAATAATGCTGAGTTAAGGAAACTTTTGATTGGAATCACGAGTAAATCTGTTGTGGTTATTGAAGATATTGATTGTTCGCTTGATCTTACCGGTCAGAGGAAGACAGATTCGGAGAATGACAGAGAAAAAGAGGACAAAAATGAGGAGGTGAATCAAGTTGCTGCTGCTTCTCTGCAGGGATTACAAGCAGCAGATAAAGCAAAGAACAAAGCAAGTCAGGTAACACTTTCTGGGTTATTGAATTTCATTGATGGGATTTGGTCTGCTAGTACTGGTGAGAGATTGATTATATTCACTACTAACTATGTGGAGAAACTTGATCAAGCTTTGATTCGTCGTGGACGAATGGACATGCATATTGAGTTGTCTTATTGTGGTTTTGATGGATTCAAAATGCTGGCAATGAATTATCTGAGTATTGAATCTCATCCTTTGTTCGAGATGGTTCGGTCCTTGTTGGAAGAGACTAACATGACTCCAGCTGATGTTGCTGAAAACTTGATGCCTAAGGTAGCTGATGAAGATGTTGAGGCATCATTAGAGAGGTTGATCCAAGCACTTAGAACCTCTAAGGAAGAGGCTGAGATGAAAGCTAAGAAAGAAGCAGAGATGAAGGCTGAGAAAGAAGAAGGGACAGGTGAAGAGGATTCATCAGAGAAGGAGGCAGAAGATGCAGAAGACAAGAAGCCTAAGGAAGAGAATGCCAATGAAAATTCCTAG |
| Protein sequence |
>MsG0180000154.01.T01 MFELWGLIGSKLASLMFIWAMIQSYCPHQVHALIEKFSEKLANFCYPFVEVRFFENIGDYMKTNEAFLYIENYLTSKSTNQAKKLQGEYARKSLVLKMDERQKFYDEFEGVKFVWSLIKIVPNTNSVSFYPASDKRFYLLTFHRSHRDFVEKSYLNHVLEQGKEIGLSKRQRKLYTNCTGNGEYERGGKWSHVIFEHPSSFETIAMNPKKKKEIVDDLVTFSKAKEYYAKIGKPWKRGYLLYGPPGTGKSSLVAAIANFLKYDIYDIELTNVKNNAELRKLLIGITSKSVVVIEDIDCSLDLTGQRKTDSENDREKEDKNEEVNQVAAASLQGLQAADKAKNKASQVTLSGLLNFIDGIWSASTGERLIIFTTNYVEKLDQALIRRGRMDMHIELSYCGFDGFKMLAMNYLSIESHPLFEMVRSLLEETNMTPADVAENLMPKVADEDVEASLERLIQALRTSKEEAEMKAKKEAEMKAEKEEGTGEEDSSEKEAEDAEDKKPKEENANENS* |
