AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000702.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000702.01.T01 MTR_1g010090 95.238 63 3 0 26 88 137 199 3.63e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCTCTTACTAGGCTTTAC+TGG 0.278322 1:-9943661 None:intergenic
TCTGAATCATGCCTCTTACT+AGG 0.419991 1:-9943670 None:intergenic
CTAAGCACCGCAAGAAAGGC+AGG 0.463539 1:+9943563 MsG0180000702.01.T01:CDS
TCCTTCACTAGTCCTACGCC+TGG 0.470161 1:-9943722 None:intergenic
AACCAGTAAAGCCTAGTAAG+AGG 0.471344 1:+9943659 MsG0180000702.01.T01:CDS
ATCATCATGAAGGCGTGCCC+AGG 0.502347 1:+9943704 MsG0180000702.01.T01:CDS
CTTCCACCCTGCCTTTCTTG+CGG 0.518312 1:-9943570 None:intergenic
ACATCTAAGCACCGCAAGAA+AGG 0.532339 1:+9943559 MsG0180000702.01.T01:CDS
CGTAGGACTAGTGAAGGAAG+AGG 0.555786 1:+9943727 MsG0180000702.01.T01:CDS
ATGAAGGCGTGCCCAGGCGT+AGG 0.565815 1:+9943710 MsG0180000702.01.T01:CDS
ATTCTGATAGTGATGCTAAG+AGG 0.583073 1:+9943509 MsG0180000702.01.T01:CDS
CCCAGGCGTAGGACTAGTGA+AGG 0.592935 1:+9943721 MsG0180000702.01.T01:CDS
ATCAGATTCATCACTACTGT+CGG 0.593798 1:-9943488 None:intergenic
AGTAAGAGGCATGATTCAGA+TGG 0.597582 1:+9943673 MsG0180000702.01.T01:CDS
TGATTTCTCTTCTGATAGTG+AGG 0.602201 1:+9943606 MsG0180000702.01.T01:CDS
GGAGATTCTGATCATCATGA+AGG 0.612338 1:+9943694 MsG0180000702.01.T01:CDS
CCTTCACTAGTCCTACGCCT+GGG 0.620271 1:-9943721 None:intergenic
TGATAGTGATGCTAAGAGGA+AGG 0.626197 1:+9943513 MsG0180000702.01.T01:CDS
TGATGCTAAGAGGAAGGTCA+AGG 0.645161 1:+9943519 MsG0180000702.01.T01:CDS
GCAAACATCATGATTCGAAG+AGG 0.655029 1:+9943763 MsG0180000702.01.T01:CDS
TAAGCACCGCAAGAAAGGCA+GGG 0.680248 1:+9943564 MsG0180000702.01.T01:CDS
GCACCGCAAGAAAGGCAGGG+TGG 0.713680 1:+9943567 MsG0180000702.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCAGATTCATCACTACTGT+CGG - Chr1:9943491-9943510 None:intergenic 35.0%
! ATTCTGATAGTGATGCTAAG+AGG + Chr1:9943509-9943528 MsG0180000702.01.T01:CDS 35.0%
! TGATTTCTCTTCTGATAGTG+AGG + Chr1:9943606-9943625 MsG0180000702.01.T01:CDS 35.0%
AACCAGTAAAGCCTAGTAAG+AGG + Chr1:9943659-9943678 MsG0180000702.01.T01:CDS 40.0%
GCAAACATCATGATTCGAAG+AGG + Chr1:9943763-9943782 MsG0180000702.01.T01:CDS 40.0%
GGAGATTCTGATCATCATGA+AGG + Chr1:9943694-9943713 MsG0180000702.01.T01:CDS 40.0%
TCTGAATCATGCCTCTTACT+AGG - Chr1:9943673-9943692 None:intergenic 40.0%
! AGTAAGAGGCATGATTCAGA+TGG + Chr1:9943673-9943692 MsG0180000702.01.T01:CDS 40.0%
! TGATAGTGATGCTAAGAGGA+AGG + Chr1:9943513-9943532 MsG0180000702.01.T01:CDS 40.0%
TGATGCTAAGAGGAAGGTCA+AGG + Chr1:9943519-9943538 MsG0180000702.01.T01:CDS 45.0%
TGCCTCTTACTAGGCTTTAC+TGG - Chr1:9943664-9943683 None:intergenic 45.0%
! ACATCTAAGCACCGCAAGAA+AGG + Chr1:9943559-9943578 MsG0180000702.01.T01:CDS 45.0%
! CGTAGGACTAGTGAAGGAAG+AGG + Chr1:9943727-9943746 MsG0180000702.01.T01:CDS 50.0%
! TAAGCACCGCAAGAAAGGCA+GGG + Chr1:9943564-9943583 MsG0180000702.01.T01:CDS 50.0%
CCTTCACTAGTCCTACGCCT+GGG - Chr1:9943724-9943743 None:intergenic 55.0%
CTTCCACCCTGCCTTTCTTG+CGG - Chr1:9943573-9943592 None:intergenic 55.0%
TCCTTCACTAGTCCTACGCC+TGG - Chr1:9943725-9943744 None:intergenic 55.0%
! ATCATCATGAAGGCGTGCCC+AGG + Chr1:9943704-9943723 MsG0180000702.01.T01:CDS 55.0%
! CTAAGCACCGCAAGAAAGGC+AGG + Chr1:9943563-9943582 MsG0180000702.01.T01:CDS 55.0%
! CCCAGGCGTAGGACTAGTGA+AGG + Chr1:9943721-9943740 MsG0180000702.01.T01:CDS 60.0%
GCACCGCAAGAAAGGCAGGG+TGG + Chr1:9943567-9943586 MsG0180000702.01.T01:CDS 65.0%
! ATGAAGGCGTGCCCAGGCGT+AGG + Chr1:9943710-9943729 MsG0180000702.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 9943478 9943792 9943478 ID=MsG0180000702.01;Name=MsG0180000702.01
Chr1 mRNA 9943478 9943792 9943478 ID=MsG0180000702.01.T01;Parent=MsG0180000702.01;Name=MsG0180000702.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|104
Chr1 exon 9943478 9943792 9943478 ID=MsG0180000702.01.T01:exon:1728;Parent=MsG0180000702.01.T01
Chr1 CDS 9943478 9943792 9943478 ID=MsG0180000702.01.T01:cds;Parent=MsG0180000702.01.T01
Gene Sequence

>MsG0180000702.01.T01

ATGAGGAGTACCGACAGTAGTGATGAATCTGATTCTGATAGTGATGCTAAGAGGAAGGTCAAGGCAAAAAAAGAGCAGAGCACATCTAAGCACCGCAAGAAAGGCAGGGTGGAAGACAGTGATGATACTGATTTCTCTTCTGATAGTGAGGATAGCAAGAGTGCAAGAAAAAGTAATAAAAAACCAGTAAAGCCTAGTAAGAGGCATGATTCAGATGGAGATTCTGATCATCATGAAGGCGTGCCCAGGCGTAGGACTAGTGAAGGAAGAGGCATGCAAAGACGAGCAAACATCATGATTCGAAGAGGAATCTGA

Protein sequence

>MsG0180000702.01.T01

MRSTDSSDESDSDSDAKRKVKAKKEQSTSKHRKKGRVEDSDDTDFSSDSEDSKSARKSNKKPVKPSKRHDSDGDSDHHEGVPRRRTSEGRGMQRRANIMIRRGI*