AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000510.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000510.01.T01 MTR_1g013660 100.000 178 0 0 1 178 1 178 7.57e-130 361
MsG0180000510.01.T01 MTR_8g027900 100.000 51 0 0 61 111 156 206 1.63e-27 108
MsG0180000510.01.T01 MTR_8g027900 96.875 32 1 0 1 32 1 32 6.84e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000510.01.T01 AT5G10780 81.768 181 29 2 1 178 1 180 3.51e-88 256
MsG0180000510.01.T01 AT5G10780 82.840 169 25 2 1 166 1 168 8.17e-82 240

Find 93 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTCTTGGAGGAATTTA+AGG 0.091841 1:+7172166 MsG0180000510.01.T01:three_prime_UTR
GGTATTCATCGCCCTTAATT+TGG 0.174653 1:+7170940 MsG0180000510.01.T01:CDS
TCTTCAGCGATTCAATTAAA+TGG 0.185762 1:+7169251 MsG0180000510.01.T01:exon
AACACTCTGTAAGGCACTTA+TGG 0.194375 1:-7170000 None:intergenic
TACTGCAAATCTGATGTAAA+AGG 0.202542 1:+7172409 MsG0180000510.01.T01:three_prime_UTR
ATCATTAACAAGTTCTTAAA+TGG 0.219625 1:-7169900 None:intergenic
GTAACATGCCTCCCAAATTA+AGG 0.244098 1:-7170952 None:intergenic
CTTGGAGGAATTTAAGGATT+TGG 0.280619 1:+7172172 MsG0180000510.01.T01:three_prime_UTR
CTTCACAATGGGAAGGTTAC+AGG 0.295260 1:-7172544 None:intergenic
TGAAGCAGGTATACAAAATA+TGG 0.299528 1:+7172562 MsG0180000510.01.T01:three_prime_UTR
TGGATTCAGCATCTTTCTTT+TGG 0.301710 1:-7169413 None:intergenic
CTGAAAGAACAATAAGAATT+CGG 0.312632 1:-7172489 None:intergenic
TCTTCTGCAGCTCAATTCAT+TGG 0.332546 1:+7171350 MsG0180000510.01.T01:intron
CTTTGTATCTCTTTGTATGT+AGG 0.333936 1:+7172347 MsG0180000510.01.T01:three_prime_UTR
GTATCACCTTCTCAGCTCTT+TGG 0.334333 1:+7169973 MsG0180000510.01.T01:CDS
AAGGGTGTGATGGGATCTTC+TGG 0.346236 1:+7169282 MsG0180000510.01.T01:CDS
GTATTCATCGCCCTTAATTT+GGG 0.348550 1:+7170941 MsG0180000510.01.T01:CDS
TACAAACCATGTCTAATCTT+TGG 0.367592 1:-7172285 None:intergenic
CTCTTTCATTCATTCATAGT+TGG 0.368002 1:+7169167 None:intergenic
GATGCTAAAGTGGAGCTTCT+TGG 0.369452 1:+7170908 MsG0180000510.01.T01:CDS
AATAGCATACTAGAAATGTT+AGG 0.385259 1:+7172433 MsG0180000510.01.T01:three_prime_UTR
ATTTAAGAACTTGTTAATGA+TGG 0.394212 1:+7169902 MsG0180000510.01.T01:CDS
ATCGCGAGAAGTTGAATTAT+CGG 0.394794 1:-7169328 None:intergenic
GAGGGAAGCGCGTGAGAAAC+CGG 0.398618 1:-7169364 None:intergenic
AATTTGGGAGGCATGTTACT+AGG 0.402445 1:+7170956 MsG0180000510.01.T01:CDS
GAGGCATGTTACTAGGTGTT+TGG 0.405795 1:+7170963 MsG0180000510.01.T01:CDS
CGATTCAATTAAATGGATAA+AGG 0.406344 1:+7169258 MsG0180000510.01.T01:exon
ATGCTAAAGTGGAGCTTCTT+GGG 0.409851 1:+7170909 MsG0180000510.01.T01:CDS
TCCACTTTAGCATCTTTGTA+AGG 0.413449 1:-7170899 None:intergenic
TGAAAGAACAATAAGAATTC+GGG 0.413709 1:-7172488 None:intergenic
CTTCTGCAGCTCAATTCATT+GGG 0.415950 1:+7171351 MsG0180000510.01.T01:intron
TGGTCACAAATGATCTCATT+AGG 0.422294 1:+7172214 MsG0180000510.01.T01:three_prime_UTR
AAGAATGAGTGAAGAGAAGA+AGG 0.424461 1:-7169232 None:intergenic
ATTGTTGGAAACAGAAAGCT+TGG 0.427332 1:+7169862 MsG0180000510.01.T01:intron
ACACTCTGTAAGGCACTTAT+GGG 0.435288 1:-7169999 None:intergenic
TTTAAGAACTTGTTAATGAT+GGG 0.445845 1:+7169903 MsG0180000510.01.T01:CDS
AGAAAGATGCTGAATCCAAT+TGG 0.446637 1:+7169418 MsG0180000510.01.T01:CDS
TAACCTTCCCATTGTGAAGC+AGG 0.447117 1:+7172548 MsG0180000510.01.T01:three_prime_UTR
ATAGCATACTAGAAATGTTA+GGG 0.456027 1:+7172434 MsG0180000510.01.T01:three_prime_UTR
TAACATGCCTCCCAAATTAA+GGG 0.464691 1:-7170951 None:intergenic
GGGTTTATGATGTGGATGGC+TGG 0.475763 1:+7169924 MsG0180000510.01.T01:CDS
AACATAGACATTTAAAAGAA+AGG 0.477427 1:-7172515 None:intergenic
TTGTATACCTGCTTCACAAT+GGG 0.483638 1:-7172555 None:intergenic
TTCCTACCCACACATCAGAC+TGG 0.494006 1:+7171379 MsG0180000510.01.T01:CDS
TGGGATCTTCTGGACGAAGA+TGG 0.499581 1:+7169292 MsG0180000510.01.T01:CDS
TGAATCCAATTGGAAATCTC+AGG 0.499594 1:+7169428 MsG0180000510.01.T01:CDS
AAAGAAAATCCAACCTGAGC+AGG 0.500189 1:-7171417 None:intergenic
ACTGTTCACTTGTTTAGTAT+CGG 0.507745 1:+7169951 MsG0180000510.01.T01:CDS
CAGGAGGTGGAGTACTCAGG+TGG 0.509179 1:+7172101 MsG0180000510.01.T01:intron
GTCATCCTTAGCACCTGCTC+AGG 0.516236 1:+7171404 MsG0180000510.01.T01:CDS
GTACTGTTAGCATGTTGTAA+TGG 0.518335 1:+7172194 MsG0180000510.01.T01:three_prime_UTR
GATAAAGGAAAGGGTGTGAT+GGG 0.521901 1:+7169273 MsG0180000510.01.T01:CDS
CTTCAGGAGGTGGAGTACTC+AGG 0.524189 1:+7172098 MsG0180000510.01.T01:intron
GAGGTGGAGTACTCAGGTGG+GGG 0.524742 1:+7172104 MsG0180000510.01.T01:CDS
GGATAAAGGAAAGGGTGTGA+TGG 0.525644 1:+7169272 MsG0180000510.01.T01:CDS
GGAGCTTCTTGGGCCTAAGT+TGG 0.528352 1:+7170919 MsG0180000510.01.T01:CDS
CGCGATATTATCGATCCTCC+CGG 0.529746 1:+7169345 MsG0180000510.01.T01:CDS
ACCTTACAAAGATGCTAAAG+TGG 0.529869 1:+7170898 MsG0180000510.01.T01:CDS
GGGATCTTCTGGACGAAGAT+GGG 0.531311 1:+7169293 MsG0180000510.01.T01:CDS
GCATACCTGAGATTTCCAAT+TGG 0.533753 1:-7169433 None:intergenic
AGGAGGTGGAGTACTCAGGT+GGG 0.535718 1:+7172102 MsG0180000510.01.T01:intron
AATTAAATGGATAAAGGAAA+GGG 0.536006 1:+7169264 MsG0180000510.01.T01:exon
CATGTTACTAGGTGTTTGGA+AGG 0.537062 1:+7170967 MsG0180000510.01.T01:CDS
GAAAGCTTGGGAAGTTGCAC+AGG 0.537408 1:+7169875 MsG0180000510.01.T01:intron
TCCAACCTGAGCAGGTGCTA+AGG 0.537452 1:-7171409 None:intergenic
CAGTCACCAAAGATTAGACA+TGG 0.538585 1:+7172279 MsG0180000510.01.T01:three_prime_UTR
TCTTTGGTGACTGCAAGTAC+TGG 0.538929 1:-7172269 None:intergenic
CTTAAGAACTGGAGCATCAA+CGG 0.547901 1:-7172136 None:intergenic
GGAGGTGGAGTACTCAGGTG+GGG 0.550312 1:+7172103 MsG0180000510.01.T01:intron
TCCTACCCACACATCAGACT+GGG 0.552562 1:+7171380 MsG0180000510.01.T01:CDS
CAATTAAATGGATAAAGGAA+AGG 0.554170 1:+7169263 MsG0180000510.01.T01:exon
AGGGCGATGAATACCAACTT+AGG 0.558217 1:-7170932 None:intergenic
AGGGAAGCGCGTGAGAAACC+GGG 0.558868 1:-7169363 None:intergenic
TTGTTGGAAACAGAAAGCTT+GGG 0.565750 1:+7169863 MsG0180000510.01.T01:intron
TTAAGAACTTGTTAATGATG+GGG 0.568371 1:+7169904 MsG0180000510.01.T01:CDS
TTTGTATACCTGCTTCACAA+TGG 0.573399 1:-7172556 None:intergenic
TTCATCGCCCTTAATTTGGG+AGG 0.578157 1:+7170944 MsG0180000510.01.T01:CDS
GGGTTGCCAAAGAGCTGAGA+AGG 0.582231 1:-7169979 None:intergenic
GGTAGAATCGTCTTGATCGA+GGG 0.585755 1:-7169382 None:intergenic
AGAATGAGTGAAGAGAAGAA+GGG 0.591696 1:-7169231 None:intergenic
TAATGATGGGGTTTATGATG+TGG 0.592983 1:+7169916 MsG0180000510.01.T01:CDS
ATAAGTGCCTTACAGAGTGT+TGG 0.594768 1:+7170002 MsG0180000510.01.T01:CDS
GGGTAGAATCGTCTTGATCG+AGG 0.613210 1:-7169383 None:intergenic
TGAATTATCGGAGAAATCAA+CGG 0.616548 1:-7169316 None:intergenic
ATACCTGCTTCACAATGGGA+AGG 0.628040 1:-7172551 None:intergenic
ACATACAAAGAGATACAAAG+AGG 0.639704 1:-7172344 None:intergenic
GATGGGGTTTATGATGTGGA+TGG 0.644891 1:+7169920 MsG0180000510.01.T01:CDS
CATACTAGAAATGTTAGGGG+AGG 0.666754 1:+7172438 MsG0180000510.01.T01:three_prime_UTR
TGACACCCAGTCTGATGTGT+GGG 0.677476 1:-7171385 None:intergenic
TAGCATACTAGAAATGTTAG+GGG 0.703626 1:+7172435 MsG0180000510.01.T01:three_prime_UTR
ATGACACCCAGTCTGATGTG+TGG 0.709294 1:-7171386 None:intergenic
GAAGCGCGTGAGAAACCGGG+AGG 0.725314 1:-7169360 None:intergenic
ACCCAGTCTGATGTGTGGGT+AGG 0.740029 1:-7171381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGAACTAAATAAAAA+GGG - Chr1:7172591-7172610 None:intergenic 10.0%
!! GAAAAATAATAATAAGTATA+TGG - Chr1:7171309-7171328 None:intergenic 10.0%
!!! TTTTTGATTAATTATATTGT+TGG + Chr1:7169847-7169866 MsG0180000510.01.T01:intron 10.0%
!! AAAATACAGAAATTATACAT+AGG - Chr1:7169509-7169528 None:intergenic 15.0%
!! CAAAAATGAACTAAATAAAA+AGG - Chr1:7172592-7172611 None:intergenic 15.0%
!! TATGTTCTATAATATATTTG+AGG + Chr1:7171219-7171238 MsG0180000510.01.T01:intron 15.0%
!! TATTTAACAAAACAACAAAA+AGG - Chr1:7170062-7170081 None:intergenic 15.0%
!! TGTAATTGTATTATATGAAT+TGG + Chr1:7170205-7170224 MsG0180000510.01.T01:intron 15.0%
!! TGTATGTTATTAAAATGTTT+GGG + Chr1:7171611-7171630 MsG0180000510.01.T01:intron 15.0%
!! TTGTATGTTATTAAAATGTT+TGG + Chr1:7171610-7171629 MsG0180000510.01.T01:intron 15.0%
!! TTTATATTATGGATGAAATT+AGG + Chr1:7170282-7170301 MsG0180000510.01.T01:intron 15.0%
!! TTTGAAAATAAATCTATAAC+TGG + Chr1:7171950-7171969 MsG0180000510.01.T01:intron 15.0%
!!! AAAAAAAATCTATAACTTGT+TGG + Chr1:7171983-7172002 MsG0180000510.01.T01:intron 15.0%
!! AACATAGACATTTAAAAGAA+AGG - Chr1:7172518-7172537 None:intergenic 20.0%
!! AATTAAATGGATAAAGGAAA+GGG + Chr1:7169264-7169283 MsG0180000510.01.T01:exon 20.0%
!! AGTTACAATAATTAGTTACA+TGG - Chr1:7169773-7169792 None:intergenic 20.0%
!! ATACAATTACAATCATTGAA+TGG - Chr1:7170196-7170215 None:intergenic 20.0%
!! ATCATTAACAAGTTCTTAAA+TGG - Chr1:7169903-7169922 None:intergenic 20.0%
!! CAATGTTGATATTATTAGAA+TGG - Chr1:7170144-7170163 None:intergenic 20.0%
!! GAAAAAAAAAAAAAAACCAG+AGG + Chr1:7171655-7171674 MsG0180000510.01.T01:intron 20.0%
!! TTAATAACATACAATGCTAT+GGG - Chr1:7171604-7171623 None:intergenic 20.0%
!! TTTAATAACATACAATGCTA+TGG - Chr1:7171605-7171624 None:intergenic 20.0%
!!! AGTTATAGATTTTTTTTCAG+TGG - Chr1:7171980-7171999 None:intergenic 20.0%
!!! ATTTAAGAACTTGTTAATGA+TGG + Chr1:7169902-7169921 MsG0180000510.01.T01:CDS 20.0%
!!! CATTATTTCTGATATTAAAG+TGG - Chr1:7171924-7171943 None:intergenic 20.0%
!!! CTATAATATATTTGAGGTTT+AGG + Chr1:7171225-7171244 MsG0180000510.01.T01:intron 20.0%
!!! CTTAATATGTATTTGTTGAA+TGG + Chr1:7170558-7170577 MsG0180000510.01.T01:intron 20.0%
!!! GAACACTTTATTTTAAATTG+TGG + Chr1:7170714-7170733 MsG0180000510.01.T01:intron 20.0%
!!! GAGGTTGATTATTTATATTA+TGG + Chr1:7170271-7170290 MsG0180000510.01.T01:intron 20.0%
!!! GGTTTTTTTTTTTTTTTCCT+GGG - Chr1:7171653-7171672 None:intergenic 20.0%
!!! TGGTTTTTTTTTTTTTTTCC+TGG - Chr1:7171654-7171673 None:intergenic 20.0%
!!! TTACCTCAAATTTTAAGTTT+TGG + Chr1:7171164-7171183 MsG0180000510.01.T01:intron 20.0%
!!! TTTAACTCATTTTTGTTTCA+AGG - Chr1:7171003-7171022 None:intergenic 20.0%
!!! TTTAAGAACTTGTTAATGAT+GGG + Chr1:7169903-7169922 MsG0180000510.01.T01:CDS 20.0%
! AAAATATGTAATTGCGTGTT+TGG + Chr1:7170653-7170672 MsG0180000510.01.T01:intron 25.0%
! AAAGAAAAATAAAGTCGAAC+TGG + Chr1:7170380-7170399 MsG0180000510.01.T01:intron 25.0%
! AAGACAAAAAACTTAAGAAC+TGG - Chr1:7172150-7172169 None:intergenic 25.0%
! AATAGCATACTAGAAATGTT+AGG + Chr1:7172433-7172452 MsG0180000510.01.T01:three_prime_UTR 25.0%
! ACAAAACCATGTATATACTT+AGG - Chr1:7171144-7171163 None:intergenic 25.0%
! ACTTAAAGAAAAGAAACCAA+GGG - Chr1:7172054-7172073 None:intergenic 25.0%
! ATAGCATACTAGAAATGTTA+GGG + Chr1:7172434-7172453 MsG0180000510.01.T01:three_prime_UTR 25.0%
! ATCTTATGAAAACAACGTAT+AGG - Chr1:7170416-7170435 None:intergenic 25.0%
! ATTCTCAAGTTTGAAAATCT+TGG + Chr1:7171281-7171300 MsG0180000510.01.T01:intron 25.0%
! ATTGATAAACTCTTGATTCT+AGG + Chr1:7171097-7171116 MsG0180000510.01.T01:intron 25.0%
! ATTTATACACCAAATGATTG+TGG - Chr1:7171810-7171829 None:intergenic 25.0%
! CAATTAAATGGATAAAGGAA+AGG + Chr1:7169263-7169282 MsG0180000510.01.T01:exon 25.0%
! CATCTGATTGTAATTGTTTA+AGG + Chr1:7169801-7169820 MsG0180000510.01.T01:intron 25.0%
! CGATTCAATTAAATGGATAA+AGG + Chr1:7169258-7169277 MsG0180000510.01.T01:exon 25.0%
! CTGAAAGAACAATAAGAATT+CGG - Chr1:7172492-7172511 None:intergenic 25.0%
! GAACCAAAACTTAAAATTTG+AGG - Chr1:7171170-7171189 None:intergenic 25.0%
! TATAAGCTGAAAACAACTTA+TGG + Chr1:7170455-7170474 MsG0180000510.01.T01:intron 25.0%
! TCAACTGATAATAAACGAAA+GGG - Chr1:7169643-7169662 None:intergenic 25.0%
! TGAAAGAACAATAAGAATTC+GGG - Chr1:7172491-7172510 None:intergenic 25.0%
! TTAACAAAACAACAAAAAGG+AGG - Chr1:7170059-7170078 None:intergenic 25.0%
! TTGATAAACTCTTGATTCTA+GGG + Chr1:7171098-7171117 MsG0180000510.01.T01:intron 25.0%
! TTTATTATCAGTTGAGATGT+GGG + Chr1:7169648-7169667 MsG0180000510.01.T01:intron 25.0%
! TTTCAAGAATTGTGAAAAGA+TGG + Chr1:7170807-7170826 MsG0180000510.01.T01:intron 25.0%
!! AACAATCGTAAATAGTTTGT+TGG - Chr1:7169678-7169697 None:intergenic 25.0%
!! ACATAATTTTACTGTCTTCA+AGG + Chr1:7171521-7171540 MsG0180000510.01.T01:intron 25.0%
!! TATTTGTTGAATGGAATCAT+TGG + Chr1:7170567-7170586 MsG0180000510.01.T01:intron 25.0%
!! TTCAGAGGACTAAAATATTT+TGG - Chr1:7171481-7171500 None:intergenic 25.0%
!! TTGCTTTATTGAGATGAAAT+TGG + Chr1:7171712-7171731 MsG0180000510.01.T01:intron 25.0%
!!! CGTTTTAATTAATGTTTCAG+AGG - Chr1:7171496-7171515 None:intergenic 25.0%
!!! TAAGTTTTGGTTCATTTTGT+TGG + Chr1:7171177-7171196 MsG0180000510.01.T01:intron 25.0%
!!! TTAAGAACTTGTTAATGATG+GGG + Chr1:7169904-7169923 MsG0180000510.01.T01:CDS 25.0%
!!! TTAAGTTTTTTGTCTTCTCT+TGG + Chr1:7172154-7172173 MsG0180000510.01.T01:three_prime_UTR 25.0%
AAATCACAAAAGGATGAAGT+AGG - Chr1:7172016-7172035 None:intergenic 30.0%
AATCTACCAATTCTTCACTA+TGG - Chr1:7171846-7171865 None:intergenic 30.0%
ACATACAAAGAGATACAAAG+AGG - Chr1:7172347-7172366 None:intergenic 30.0%
ACTGTTCACTTGTTTAGTAT+CGG + Chr1:7169951-7169970 MsG0180000510.01.T01:CDS 30.0%
ATACATATTAAGACCCTGTT+TGG - Chr1:7170550-7170569 None:intergenic 30.0%
ATAGATGAACCACAATCATT+TGG + Chr1:7171798-7171817 MsG0180000510.01.T01:intron 30.0%
CACTTAAAGAAAAGAAACCA+AGG - Chr1:7172055-7172074 None:intergenic 30.0%
CTCAACTGATAATAAACGAA+AGG - Chr1:7169644-7169663 None:intergenic 30.0%
CTTACTACAAGTAATTTCTG+TGG + Chr1:7171757-7171776 MsG0180000510.01.T01:intron 30.0%
CTTTGTATCTCTTTGTATGT+AGG + Chr1:7172347-7172366 MsG0180000510.01.T01:three_prime_UTR 30.0%
GTGGTTCATCTATATTATTC+TGG - Chr1:7171791-7171810 None:intergenic 30.0%
GTTTATTATCAGTTGAGATG+TGG + Chr1:7169647-7169666 MsG0180000510.01.T01:intron 30.0%
TACAAACCATGTCTAATCTT+TGG - Chr1:7172288-7172307 None:intergenic 30.0%
TACTGCAAATCTGATGTAAA+AGG + Chr1:7172409-7172428 MsG0180000510.01.T01:three_prime_UTR 30.0%
TAGCATACTAGAAATGTTAG+GGG + Chr1:7172435-7172454 MsG0180000510.01.T01:three_prime_UTR 30.0%
TATCAGCCTAAGTATATACA+TGG + Chr1:7171135-7171154 MsG0180000510.01.T01:intron 30.0%
TCTTCAGCGATTCAATTAAA+TGG + Chr1:7169251-7169270 MsG0180000510.01.T01:exon 30.0%
TGAAGCAGGTATACAAAATA+TGG + Chr1:7172562-7172581 MsG0180000510.01.T01:three_prime_UTR 30.0%
TGAATTATCGGAGAAATCAA+CGG - Chr1:7169319-7169338 None:intergenic 30.0%
! CTTGCGATGTTAAATCTTTT+AGG + Chr1:7170737-7170756 MsG0180000510.01.T01:intron 30.0%
! GTTCATTTTGTTGGATAGTT+GGG + Chr1:7171186-7171205 MsG0180000510.01.T01:intron 30.0%
! TGGATGAAATTAGGTTTGTT+TGG + Chr1:7170291-7170310 MsG0180000510.01.T01:intron 30.0%
!! TTGGTTTGTTGTAACTCATT+AGG + Chr1:7170618-7170637 MsG0180000510.01.T01:intron 30.0%
!!! AGGAGAATTTTTTTAGCAGT+GGG + Chr1:7172458-7172477 MsG0180000510.01.T01:three_prime_UTR 30.0%
AAACCAAAAGAGAAGAGAAC+TGG - Chr1:7170605-7170624 None:intergenic 35.0%
AACCAAAAGAGAAGAGAACT+GGG - Chr1:7170604-7170623 None:intergenic 35.0%
AAGAATGAGTGAAGAGAAGA+AGG - Chr1:7169235-7169254 None:intergenic 35.0%
AAGATATCCTAGTGAGCTTA+TGG + Chr1:7170431-7170450 MsG0180000510.01.T01:intron 35.0%
ACAAAACAACAAAAAGGAGG+CGG - Chr1:7170056-7170075 None:intergenic 35.0%
ACCTTACAAAGATGCTAAAG+TGG + Chr1:7170898-7170917 MsG0180000510.01.T01:CDS 35.0%
ACTGATAATAAACGAAAGGG+AGG - Chr1:7169640-7169659 None:intergenic 35.0%
AGAAAGATGCTGAATCCAAT+TGG + Chr1:7169418-7169437 MsG0180000510.01.T01:CDS 35.0%
AGAATGAGTGAAGAGAAGAA+GGG - Chr1:7169234-7169253 None:intergenic 35.0%
AGGCAGTAACATGAATAACA+GGG - Chr1:7171331-7171350 None:intergenic 35.0%
ATCGCGAGAAGTTGAATTAT+CGG - Chr1:7169331-7169350 None:intergenic 35.0%
ATTGTTGGAAACAGAAAGCT+TGG + Chr1:7169862-7169881 MsG0180000510.01.T01:intron 35.0%
CAAATAAGTCGATCCAAACA+GGG + Chr1:7170534-7170553 MsG0180000510.01.T01:intron 35.0%
CATATGCCATAGTGAAGAAT+TGG + Chr1:7171837-7171856 MsG0180000510.01.T01:intron 35.0%
CCTGAAGATACGAAAAAGTA+AGG - Chr1:7172085-7172104 None:intergenic 35.0%
CTTATATCCATAAGCTCACT+AGG - Chr1:7170441-7170460 None:intergenic 35.0%
CTTGATGTCTATAGATAACG+AGG + Chr1:7170252-7170271 MsG0180000510.01.T01:intron 35.0%
CTTGGAGGAATTTAAGGATT+TGG + Chr1:7172172-7172191 MsG0180000510.01.T01:three_prime_UTR 35.0%
GTACTGTTAGCATGTTGTAA+TGG + Chr1:7172194-7172213 MsG0180000510.01.T01:three_prime_UTR 35.0%
GTATTCATCGCCCTTAATTT+GGG + Chr1:7170941-7170960 MsG0180000510.01.T01:CDS 35.0%
TAACATACAATGCTATGGGT+AGG - Chr1:7171600-7171619 None:intergenic 35.0%
TAACATGCCTCCCAAATTAA+GGG - Chr1:7170954-7170973 None:intergenic 35.0%
TCAAATAAGTCGATCCAAAC+AGG + Chr1:7170533-7170552 MsG0180000510.01.T01:intron 35.0%
TCCACTTTAGCATCTTTGTA+AGG - Chr1:7170902-7170921 None:intergenic 35.0%
TCTTCTCTTGGAGGAATTTA+AGG + Chr1:7172166-7172185 MsG0180000510.01.T01:three_prime_UTR 35.0%
TGAATCCAATTGGAAATCTC+AGG + Chr1:7169428-7169447 MsG0180000510.01.T01:CDS 35.0%
TGGTCACAAATGATCTCATT+AGG + Chr1:7172214-7172233 MsG0180000510.01.T01:three_prime_UTR 35.0%
TTGAATGGAGAGATTCAGTA+AGG - Chr1:7170181-7170200 None:intergenic 35.0%
TTGTATACCTGCTTCACAAT+GGG - Chr1:7172558-7172577 None:intergenic 35.0%
TTGTTGGAAACAGAAAGCTT+GGG + Chr1:7169863-7169882 MsG0180000510.01.T01:intron 35.0%
TTTGTATACCTGCTTCACAA+TGG - Chr1:7172559-7172578 None:intergenic 35.0%
! ATCTTTCTTTTGGCGACTAA+GGG - Chr1:7169406-7169425 None:intergenic 35.0%
! CTTCAAGGTATTATGACAAG+AGG + Chr1:7171536-7171555 MsG0180000510.01.T01:intron 35.0%
! GGTTCATTTTGTTGGATAGT+TGG + Chr1:7171185-7171204 MsG0180000510.01.T01:intron 35.0%
! GTCAGTCTCATAATTGCTTT+TGG + Chr1:7170498-7170517 MsG0180000510.01.T01:intron 35.0%
! TAATGATGGGGTTTATGATG+TGG + Chr1:7169916-7169935 MsG0180000510.01.T01:CDS 35.0%
! TGGATTCAGCATCTTTCTTT+TGG - Chr1:7169416-7169435 None:intergenic 35.0%
!! ACTTTTTCCAACACTCTGTA+AGG - Chr1:7170012-7170031 None:intergenic 35.0%
!!! AGTTTTTTGTCTTCTCTTGG+AGG + Chr1:7172157-7172176 MsG0180000510.01.T01:three_prime_UTR 35.0%
!!! CCTTACTTTTTCGTATCTTC+AGG + Chr1:7172082-7172101 MsG0180000510.01.T01:intron 35.0%
!!! GAGGAGAATTTTTTTAGCAG+TGG + Chr1:7172457-7172476 MsG0180000510.01.T01:three_prime_UTR 35.0%
!!! GGAGAATTTTTTTAGCAGTG+GGG + Chr1:7172459-7172478 MsG0180000510.01.T01:three_prime_UTR 35.0%
!!! TACTTTTTCGTATCTTCAGG+AGG + Chr1:7172085-7172104 MsG0180000510.01.T01:intron 35.0%
!!! TCTTTTAGGACCTTTGCAAT+TGG + Chr1:7170751-7170770 MsG0180000510.01.T01:intron 35.0%
AAAGAAAATCCAACCTGAGC+AGG - Chr1:7171420-7171439 None:intergenic 40.0%
AAGCATGTAATCATTGCCTC+TGG - Chr1:7171674-7171693 None:intergenic 40.0%
AATGAATTGAGCTGCAGAAG+AGG - Chr1:7171351-7171370 None:intergenic 40.0%
AGGCTGATAAATTCACTGCT+AGG - Chr1:7171124-7171143 None:intergenic 40.0%
ATAAGTGCCTTACAGAGTGT+TGG + Chr1:7170002-7170021 MsG0180000510.01.T01:CDS 40.0%
ATCCACAGTTCCAATTGCAA+AGG - Chr1:7170764-7170783 None:intergenic 40.0%
ATGCTAAAGTGGAGCTTCTT+GGG + Chr1:7170909-7170928 MsG0180000510.01.T01:CDS 40.0%
CAGCTCATGGAAATCACAAA+AGG - Chr1:7172026-7172045 None:intergenic 40.0%
CAGTCACCAAAGATTAGACA+TGG + Chr1:7172279-7172298 MsG0180000510.01.T01:three_prime_UTR 40.0%
CATACTAGAAATGTTAGGGG+AGG + Chr1:7172438-7172457 MsG0180000510.01.T01:three_prime_UTR 40.0%
CATGTTACTAGGTGTTTGGA+AGG + Chr1:7170967-7170986 MsG0180000510.01.T01:CDS 40.0%
CTTCTGCAGCTCAATTCATT+GGG + Chr1:7171351-7171370 MsG0180000510.01.T01:intron 40.0%
GAGGCAGTAACATGAATAAC+AGG - Chr1:7171332-7171351 None:intergenic 40.0%
GCATACCTGAGATTTCCAAT+TGG - Chr1:7169436-7169455 None:intergenic 40.0%
GGTATTCATCGCCCTTAATT+TGG + Chr1:7170940-7170959 MsG0180000510.01.T01:CDS 40.0%
GTAACATGCCTCCCAAATTA+AGG - Chr1:7170955-7170974 None:intergenic 40.0%
TCTTCTGCAGCTCAATTCAT+TGG + Chr1:7171350-7171369 MsG0180000510.01.T01:intron 40.0%
! AACACTCTGTAAGGCACTTA+TGG - Chr1:7170003-7170022 None:intergenic 40.0%
! AATTTGGGAGGCATGTTACT+AGG + Chr1:7170956-7170975 MsG0180000510.01.T01:CDS 40.0%
! ACACTCTGTAAGGCACTTAT+GGG - Chr1:7170002-7170021 None:intergenic 40.0%
! ATCCCAGTTCTCTTCTCTTT+TGG + Chr1:7170599-7170618 MsG0180000510.01.T01:intron 40.0%
! CATCTTTCTTTTGGCGACTA+AGG - Chr1:7169407-7169426 None:intergenic 40.0%
! CTTAAGAACTGGAGCATCAA+CGG - Chr1:7172139-7172158 None:intergenic 40.0%
! GATAAAGGAAAGGGTGTGAT+GGG + Chr1:7169273-7169292 MsG0180000510.01.T01:CDS 40.0%
! TTTTTCGTATCTTCAGGAGG+TGG + Chr1:7172088-7172107 MsG0180000510.01.T01:intron 40.0%
AGGGCGATGAATACCAACTT+AGG - Chr1:7170935-7170954 None:intergenic 45.0%
ATACCTGCTTCACAATGGGA+AGG - Chr1:7172554-7172573 None:intergenic 45.0%
ATCCGTCAATCATTCCCGTT+CGG - Chr1:7169735-7169754 None:intergenic 45.0%
ATCTTCACCTGAGATCTCGT+AGG - Chr1:7170119-7170138 None:intergenic 45.0%
CTCAACTCATAGATGCCGAA+CGG + Chr1:7169717-7169736 MsG0180000510.01.T01:intron 45.0%
CTTCACAATGGGAAGGTTAC+AGG - Chr1:7172547-7172566 None:intergenic 45.0%
GACCTTTGCAATTGGAACTG+TGG + Chr1:7170759-7170778 MsG0180000510.01.T01:intron 45.0%
GAGGCATGTTACTAGGTGTT+TGG + Chr1:7170963-7170982 MsG0180000510.01.T01:CDS 45.0%
GATGCTAAAGTGGAGCTTCT+TGG + Chr1:7170908-7170927 MsG0180000510.01.T01:CDS 45.0%
GATGGGGTTTATGATGTGGA+TGG + Chr1:7169920-7169939 MsG0180000510.01.T01:CDS 45.0%
GCAACAATATCTGTAGACCC+AGG + Chr1:7171633-7171652 MsG0180000510.01.T01:intron 45.0%
GGTAGAATCGTCTTGATCGA+GGG - Chr1:7169385-7169404 None:intergenic 45.0%
GTAGGTGTGTGAGTTTCAAG+TGG - Chr1:7170101-7170120 None:intergenic 45.0%
GTATCACCTTCTCAGCTCTT+TGG + Chr1:7169973-7169992 MsG0180000510.01.T01:CDS 45.0%
TAACCTTCCCATTGTGAAGC+AGG + Chr1:7172548-7172567 MsG0180000510.01.T01:three_prime_UTR 45.0%
TCAACTCATAGATGCCGAAC+GGG + Chr1:7169718-7169737 MsG0180000510.01.T01:intron 45.0%
! GGATAAAGGAAAGGGTGTGA+TGG + Chr1:7169272-7169291 MsG0180000510.01.T01:CDS 45.0%
! TCTTTGGTGACTGCAAGTAC+TGG - Chr1:7172272-7172291 None:intergenic 45.0%
! TTCATCGCCCTTAATTTGGG+AGG + Chr1:7170944-7170963 MsG0180000510.01.T01:CDS 45.0%
ATGACACCCAGTCTGATGTG+TGG - Chr1:7171389-7171408 None:intergenic 50.0%
CGCGATATTATCGATCCTCC+CGG + Chr1:7169345-7169364 MsG0180000510.01.T01:CDS 50.0%
GAAAGCTTGGGAAGTTGCAC+AGG + Chr1:7169875-7169894 MsG0180000510.01.T01:intron 50.0%
GGGATCTTCTGGACGAAGAT+GGG + Chr1:7169293-7169312 MsG0180000510.01.T01:CDS 50.0%
GGGTAGAATCGTCTTGATCG+AGG - Chr1:7169386-7169405 None:intergenic 50.0%
GGGTTTATGATGTGGATGGC+TGG + Chr1:7169924-7169943 MsG0180000510.01.T01:CDS 50.0%
TCACACACCTACGAGATCTC+AGG + Chr1:7170109-7170128 MsG0180000510.01.T01:intron 50.0%
TCCTACCCACACATCAGACT+GGG + Chr1:7171380-7171399 MsG0180000510.01.T01:CDS 50.0%
TGACACCCAGTCTGATGTGT+GGG - Chr1:7171388-7171407 None:intergenic 50.0%
TGCCGAACGGGAATGATTGA+CGG + Chr1:7169730-7169749 MsG0180000510.01.T01:intron 50.0%
TGGGATCTTCTGGACGAAGA+TGG + Chr1:7169292-7169311 MsG0180000510.01.T01:CDS 50.0%
TTCCTACCCACACATCAGAC+TGG + Chr1:7171379-7171398 MsG0180000510.01.T01:CDS 50.0%
! AAGGGTGTGATGGGATCTTC+TGG + Chr1:7169282-7169301 MsG0180000510.01.T01:CDS 50.0%
ACCCAGTCTGATGTGTGGGT+AGG - Chr1:7171384-7171403 None:intergenic 55.0%
GGAGCTTCTTGGGCCTAAGT+TGG + Chr1:7170919-7170938 MsG0180000510.01.T01:CDS 55.0%
GGGTTGCCAAAGAGCTGAGA+AGG - Chr1:7169982-7170001 None:intergenic 55.0%
GTCATCCTTAGCACCTGCTC+AGG + Chr1:7171404-7171423 MsG0180000510.01.T01:CDS 55.0%
TCCAACCTGAGCAGGTGCTA+AGG - Chr1:7171412-7171431 None:intergenic 55.0%
TCCATGAGCTGCAACACCCT+TGG + Chr1:7172035-7172054 MsG0180000510.01.T01:intron 55.0%
TCCTTAGCACCTGCTCAGGT+TGG + Chr1:7171408-7171427 MsG0180000510.01.T01:intron 55.0%
! AGGAGGTGGAGTACTCAGGT+GGG + Chr1:7172102-7172121 MsG0180000510.01.T01:intron 55.0%
! CTTCAGGAGGTGGAGTACTC+AGG + Chr1:7172098-7172117 MsG0180000510.01.T01:intron 55.0%
!! ACCAAGGGTGTTGCAGCTCA+TGG - Chr1:7172039-7172058 None:intergenic 55.0%
! AGGGAAGCGCGTGAGAAACC+GGG - Chr1:7169366-7169385 None:intergenic 60.0%
! CAGGAGGTGGAGTACTCAGG+TGG + Chr1:7172101-7172120 MsG0180000510.01.T01:intron 60.0%
! GAGGGAAGCGCGTGAGAAAC+CGG - Chr1:7169367-7169386 None:intergenic 60.0%
! GAGGTGGAGTACTCAGGTGG+GGG + Chr1:7172104-7172123 MsG0180000510.01.T01:CDS 60.0%
! GGAGGTGGAGTACTCAGGTG+GGG + Chr1:7172103-7172122 MsG0180000510.01.T01:intron 60.0%
GAAGCGCGTGAGAAACCGGG+AGG - Chr1:7169363-7169382 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 7169174 7172612 7169174 ID=MsG0180000510.01;Name=MsG0180000510.01
Chr1 mRNA 7169174 7172612 7169174 ID=MsG0180000510.01.T01;Parent=MsG0180000510.01;Name=MsG0180000510.01.T01;_AED=0.34;_eAED=0.34;_QI=96|1|1|1|1|1|5|470|178
Chr1 exon 7172104 7172612 7172104 ID=MsG0180000510.01.T01:exon:27787;Parent=MsG0180000510.01.T01
Chr1 exon 7171360 7171425 7171360 ID=MsG0180000510.01.T01:exon:27786;Parent=MsG0180000510.01.T01
Chr1 exon 7170891 7170988 7170891 ID=MsG0180000510.01.T01:exon:27785;Parent=MsG0180000510.01.T01
Chr1 exon 7169876 7170029 7169876 ID=MsG0180000510.01.T01:exon:27784;Parent=MsG0180000510.01.T01
Chr1 exon 7169174 7169449 7169174 ID=MsG0180000510.01.T01:exon:27783;Parent=MsG0180000510.01.T01
Chr1 five_prime_UTR 7169174 7169269 7169174 ID=MsG0180000510.01.T01:five_prime_utr;Parent=MsG0180000510.01.T01
Chr1 CDS 7169270 7169449 7169270 ID=MsG0180000510.01.T01:cds;Parent=MsG0180000510.01.T01
Chr1 CDS 7169876 7170029 7169876 ID=MsG0180000510.01.T01:cds;Parent=MsG0180000510.01.T01
Chr1 CDS 7170891 7170988 7170891 ID=MsG0180000510.01.T01:cds;Parent=MsG0180000510.01.T01
Chr1 CDS 7171360 7171425 7171360 ID=MsG0180000510.01.T01:cds;Parent=MsG0180000510.01.T01
Chr1 CDS 7172104 7172142 7172104 ID=MsG0180000510.01.T01:cds;Parent=MsG0180000510.01.T01
Chr1 three_prime_UTR 7172143 7172612 7172143 ID=MsG0180000510.01.T01:three_prime_utr;Parent=MsG0180000510.01.T01
Gene Sequence

>MsG0180000510.01.T01

ATGGATAAAGGAAAGGGTGTGATGGGATCTTCTGGACGAAGATGGGCCGTTGATTTCTCCGATAATTCAACTTCTCGCGATATTATCGATCCTCCCGGTTTCTCACGCGCTTCCCTCGATCAAGACGATTCTACCCTTAGTCGCCAAAAGAAAGATGCTGAATCCAATTGGAAATCTCAGAAAGCTTGGGAAGTTGCACAGGCACCATTTAAGAACTTGTTAATGATGGGGTTTATGATGTGGATGGCTGGTAATACTGTTCACTTGTTTAGTATCGGTATCACCTTCTCAGCTCTTTGGCAACCCATAAGTGCCTTACAGAGTGTTGGAAAAATGTTTGAACCTTACAAAGATGCTAAAGTGGAGCTTCTTGGGCCTAAGTTGGTATTCATCGCCCTTAATTTGGGAGGCATGTTACTAGGTGTTTGGAAGCTCAATTCATTGGGACTGCTTCCTACCCACACATCAGACTGGGTGTCATCCTTAGCACCTGCTCAGGAGGTGGAGTACTCAGGTGGGGGTCTAAATTTCCGTTGA
Protein sequence

>MsG0180000510.01.T01

MDKGKGVMGSSGRRWAVDFSDNSTSRDIIDPPGFSRASLDQDDSTLSRQKKDAESNWKSQKAWEVAQAPFKNLLMMGFMMWMAGNTVHLFSIGITFSALWQPISALQSVGKMFEPYKDAKVELLGPKLVFIALNLGGMLLGVWKLNSLGLLPTHTSDWVSSLAPAQEVEYSGGGLNFR*