AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021265.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021265.01.T01 MTR_4g070400 89.401 217 3 1 1 217 1 197 2.19e-136 380
MsG0480021265.01.T01 MTR_4g094930 70.115 174 52 0 6 179 2 175 3.41e-89 261
MsG0480021265.01.T01 MTR_5g016560 64.607 178 63 0 3 180 14 191 4.47e-79 235
MsG0480021265.01.T01 MTR_5g016560 64.407 177 63 0 3 179 14 190 1.53e-78 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021265.01.T01 AT2G21600 67.742 217 48 3 1 217 1 195 4.24e-99 286
MsG0480021265.01.T01 AT4G39220 67.281 217 45 3 1 217 1 191 1.52e-98 285
MsG0480021265.01.T01 AT4G39220 67.281 217 45 3 1 217 1 191 1.52e-98 285
MsG0480021265.01.T01 AT2G18240 54.091 220 95 4 1 217 1 217 7.02e-75 226
MsG0480021265.01.T01 AT2G18240 53.182 220 96 4 1 217 1 216 3.94e-74 224
MsG0480021265.01.T01 AT2G23310 60.479 167 65 1 13 179 34 199 7.95e-72 218
MsG0480021265.01.T01 AT2G23310 60.479 167 65 1 13 179 34 199 8.12e-72 218

Find 78 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCGTCCATATATGGTTTA+AGG 0.143178 4:-56080216 MsG0480021265.01.T01:CDS
GAAGCTATTTCAGCATTATT+TGG 0.176959 4:-56080287 MsG0480021265.01.T01:CDS
TTTAATTTATTGCAGAAATA+TGG 0.237994 4:-56078419 MsG0480021265.01.T01:intron
GATTGTACTATCATGATTTC+AGG 0.242533 4:-56078097 MsG0480021265.01.T01:three_prime_UTR
CCACACCACATTCTACTTAT+AGG 0.263858 4:-56080258 MsG0480021265.01.T01:CDS
TATTTGATGTTCCTGTCTTC+TGG 0.273588 4:-56078647 MsG0480021265.01.T01:CDS
ATACTCTGACTACAAATAAA+AGG 0.292583 4:+56078270 None:intergenic
GGCCAATATTACTCTGCTAC+TGG 0.299673 4:-56078626 MsG0480021265.01.T01:CDS
TATTTGTAGTCAGAGTATTA+AGG 0.325613 4:-56078265 MsG0480021265.01.T01:three_prime_UTR
TTGTGGGGATTCAGGGTACT+TGG 0.335490 4:-56078299 MsG0480021265.01.T01:three_prime_UTR
TTTGTTATTGCGTCCATATA+TGG 0.340251 4:-56080223 MsG0480021265.01.T01:CDS
TACATTGTGTCTTATGGATT+GGG 0.344860 4:-56080172 MsG0480021265.01.T01:CDS
ACCCAGTAGCAGAGTAATAT+TGG 0.355701 4:+56078624 None:intergenic
TGCGTCCATATATGGTTTAA+GGG 0.360948 4:-56080215 MsG0480021265.01.T01:CDS
GATTGGGTTTCTGTCTCCTT+TGG 0.380625 4:-56080128 MsG0480021265.01.T01:CDS
GTCGTAGGGTAAATAATTAA+AGG 0.384141 4:+56078501 None:intergenic
TCAGGAAGCCGACGAATAAA+CGG 0.388525 4:+56080037 None:intergenic
TCCTTTGGTTGATCCTGAGC+TGG 0.392280 4:-56080113 MsG0480021265.01.T01:CDS
CATTCTACTTATAGGTGGAT+TGG 0.394933 4:-56080250 MsG0480021265.01.T01:CDS
GGAGGGATCGGAGGTGGAGA+AGG 0.397065 4:+56080807 None:intergenic
AGAACACAGATCTCAAAGTT+AGG 0.400484 4:-56078324 MsG0480021265.01.T01:three_prime_UTR
ACCAGCATTAGCATGTCTTC+TGG 0.405110 4:+56078194 None:intergenic
CGATTTGGCGTCTCATTGTA+AGG 0.414685 4:+56078589 None:intergenic
TTACATTGTGTCTTATGGAT+TGG 0.423570 4:-56080173 MsG0480021265.01.T01:CDS
GGACCTTTGTTGCCTACTAA+AGG 0.425663 4:-56080076 MsG0480021265.01.T01:CDS
TACATGCTGAATCTGTTGAT+TGG 0.431412 4:-56080145 MsG0480021265.01.T01:CDS
TGGAGAAGGAAGAGGATTGG+AGG 0.435036 4:+56080821 None:intergenic
TCCGTTCAGCTTGGGAAAGC+AGG 0.436653 4:-56078541 MsG0480021265.01.T01:CDS
GCAATAGTCATATCCTGAAC+AGG 0.441049 4:+56078046 None:intergenic
AGATATATTCCGTTCAGCTT+GGG 0.449734 4:-56078549 MsG0480021265.01.T01:CDS
ACATGCTGAATCTGTTGATT+GGG 0.451041 4:-56080144 MsG0480021265.01.T01:CDS
CTGCTGCTGCACCGCCACAA+TGG 0.454675 4:-56080324 MsG0480021265.01.T01:CDS
AGTAATATTGGCCAGAAGAC+AGG 0.455434 4:+56078636 None:intergenic
ATTCTACTTATAGGTGGATT+GGG 0.457018 4:-56080249 MsG0480021265.01.T01:CDS
ATCGGAGGTGGAGAAGGAAG+AGG 0.469982 4:+56080813 None:intergenic
ATTTGATCATGTGTGCGATT+TGG 0.470026 4:+56078574 None:intergenic
GGCTTCCTGAGTTTAAGTTC+TGG 0.475777 4:-56080024 MsG0480021265.01.T01:intron
AGGTGGAGAAGGAAGAGGAT+TGG 0.476212 4:+56080818 None:intergenic
CAGATATATTCCGTTCAGCT+TGG 0.477828 4:-56078550 MsG0480021265.01.T01:CDS
AACTCATCGGAGCCTTTAGT+AGG 0.483879 4:+56080064 None:intergenic
CCATCAGAAGGCTCCAGCTC+AGG 0.488403 4:+56080100 None:intergenic
GTGAATTGAATGGAAGGAAC+TGG 0.488605 4:-56080367 MsG0480021265.01.T01:exon
CTGTGAGGAGGGATCGGAGG+TGG 0.493984 4:+56080801 None:intergenic
GGAGAAGGAAGAGGATTGGA+GGG 0.502875 4:+56080822 None:intergenic
TTTACAGATTGTGAATTGAA+TGG 0.504642 4:-56080377 MsG0480021265.01.T01:intron
GAGCTGGAGCCTTCTGATGG+TGG 0.517012 4:-56080097 MsG0480021265.01.T01:CDS
CAGATTGTGAATTGAATGGA+AGG 0.524489 4:-56080373 MsG0480021265.01.T01:exon
CCTGAGCTGGAGCCTTCTGA+TGG 0.525859 4:-56080100 MsG0480021265.01.T01:CDS
GCCAATATTACTCTGCTACT+GGG 0.526550 4:-56078625 MsG0480021265.01.T01:CDS
CACCTCCGATCCCTCCTCAC+AGG 0.527013 4:-56080800 MsG0480021265.01.T01:intron
GAAGAGCTGACCTGTGAGGA+GGG 0.530975 4:+56080790 None:intergenic
GCTGACCTGTGAGGAGGGAT+CGG 0.532307 4:+56080795 None:intergenic
GTGAACAAACATATAGTCGT+AGG 0.542290 4:+56078486 None:intergenic
ACCAGAAGACATGCTAATGC+TGG 0.542714 4:-56078195 MsG0480021265.01.T01:three_prime_UTR
AGTTCAAGCCGTTTATTCGT+CGG 0.554537 4:-56080045 MsG0480021265.01.T01:CDS
AATTACCAGAACTTAAACTC+AGG 0.568313 4:+56080019 None:intergenic
ACCTGCTTTCCCAAGCTGAA+CGG 0.570448 4:+56078540 None:intergenic
AGAAGAGCTGACCTGTGAGG+AGG 0.571340 4:+56080789 None:intergenic
TAAAGATGCAGAAACTTCAA+AGG 0.571727 4:+56078024 None:intergenic
AATTTATTGCAGAAATATGG+TGG 0.578677 4:-56078416 MsG0480021265.01.T01:intron
CACCACATTCTACTTATAGG+TGG 0.584496 4:-56080255 MsG0480021265.01.T01:CDS
AACAAAGGTCCACCATCAGA+AGG 0.599128 4:+56080088 None:intergenic
CGGTGCAGCAGCAGTCGCAG+AGG 0.604794 4:+56080333 None:intergenic
TGAACAAACATATAGTCGTA+GGG 0.605778 4:+56078487 None:intergenic
TCTAAAACTGAATCAAACAC+TGG 0.614819 4:+56078147 None:intergenic
GACCTGTGAGGAGGGATCGG+AGG 0.618808 4:+56080798 None:intergenic
AATAAACGGCTTGAACTCAT+CGG 0.622551 4:+56080051 None:intergenic
TATTAAGAAAGCCTTTGTGA+AGG 0.625237 4:+56078695 None:intergenic
CTGCAGGTATTCCTTCACAA+AGG 0.628832 4:-56078706 MsG0480021265.01.T01:intron
GAGCCTTTAGTAGGCAACAA+AGG 0.632300 4:+56080073 None:intergenic
TGACACAGCAAAGCTTTAGT+CGG 0.633378 4:+56078355 None:intergenic
TCTTATGCAAGTAGTAGCAG+TGG 0.636045 4:-56078386 MsG0480021265.01.T01:CDS
TCCAGCTCAGGATCAACCAA+AGG 0.638136 4:+56080112 None:intergenic
CCTATAAGTAGAATGTGGTG+TGG 0.649825 4:+56080258 None:intergenic
AAATCTTGCAACCATTGTGG+CGG 0.660698 4:+56080313 None:intergenic
ATCCACCTATAAGTAGAATG+TGG 0.669545 4:+56080253 None:intergenic
AGAAGAAGAGCTGACCTGTG+AGG 0.683234 4:+56080786 None:intergenic
AATTGAATGGAAGGAACTGG+AGG 0.715840 4:-56080364 MsG0480021265.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTAATTTGAGTAATTTA+GGG - Chr4:56078504-56078523 MsG0480021265.01.T01:CDS 10.0%
!!! TAATTTAATTTGAGTAATTT+AGG - Chr4:56078503-56078522 MsG0480021265.01.T01:CDS 10.0%
!!! TTTTTTTATATTTGAAGTTT+TGG - Chr4:56078554-56078573 MsG0480021265.01.T01:CDS 10.0%
!! ATTTGTTTATATGTTTGAAA+CGG - Chr4:56079847-56079866 MsG0480021265.01.T01:intron 15.0%
!! TAAACAAATAAACAATGATA+AGG + Chr4:56079836-56079855 None:intergenic 15.0%
!!! AAAATAATTGATTGATAACT+TGG - Chr4:56079356-56079375 MsG0480021265.01.T01:intron 15.0%
!!! CATTTTTTTATATATGGAAA+AGG + Chr4:56079757-56079776 None:intergenic 15.0%
!!! TTTAATTTATTGCAGAAATA+TGG - Chr4:56080565-56080584 MsG0480021265.01.T01:intron 15.0%
!! CTATATATGTAGATAATTTC+GGG - Chr4:56079122-56079141 MsG0480021265.01.T01:intron 20.0%
!! GAATATCTCATTATAGTTAT+TGG - Chr4:56079928-56079947 MsG0480021265.01.T01:intron 20.0%
!! GCTATATATGTAGATAATTT+CGG - Chr4:56079121-56079140 MsG0480021265.01.T01:intron 20.0%
!! TCCAAATCATGTTTATTTAA+TGG - Chr4:56079054-56079073 MsG0480021265.01.T01:intron 20.0%
!! TCCATTAAATAAACATGATT+TGG + Chr4:56079058-56079077 None:intergenic 20.0%
!! TTATATATGGAAAAGGAATT+AGG + Chr4:56079750-56079769 None:intergenic 20.0%
!! TTATTTATTTAGTGACATTG+TGG - Chr4:56080062-56080081 MsG0480021265.01.T01:CDS 20.0%
!!! ATCAATTATTTTCAGTTGAT+CGG + Chr4:56079348-56079367 None:intergenic 20.0%
!!! TATAATGTTTCCAATTTTGT+CGG - Chr4:56079506-56079525 MsG0480021265.01.T01:intron 20.0%
!!! TTATATTTGAAGTTTTGGTT+TGG - Chr4:56078559-56078578 MsG0480021265.01.T01:CDS 20.0%
! AAAAACCCTTAAACCATATA+TGG + Chr4:56078777-56078796 None:intergenic 25.0%
! AAGAGTGAAAATTGAAGAAT+CGG + Chr4:56078291-56078310 None:intergenic 25.0%
! AATGTCGCTAAATAAATAGA+AGG + Chr4:56080031-56080050 None:intergenic 25.0%
! AATTTATTGCAGAAATATGG+TGG - Chr4:56080568-56080587 MsG0480021265.01.T01:intron 25.0%
! ACAAAAGACTTAAATCTACA+AGG - Chr4:56079887-56079906 MsG0480021265.01.T01:intron 25.0%
! ATACTCTGACTACAAATAAA+AGG + Chr4:56080717-56080736 None:intergenic 25.0%
! TAACATTTGGGAAATGAAAA+TGG + Chr4:56078261-56078280 None:intergenic 25.0%
! TCGTTATTCTATTACACAAA+CGG + Chr4:56080234-56080253 None:intergenic 25.0%
! TTTACAGATTGTGAATTGAA+TGG - Chr4:56078607-56078626 MsG0480021265.01.T01:CDS 25.0%
! TTTGTCACAATGAAAAACTA+AGG - Chr4:56079673-56079692 MsG0480021265.01.T01:intron 25.0%
!! ATGGAAGATTTGTTTGAATT+TGG - Chr4:56079073-56079092 MsG0480021265.01.T01:intron 25.0%
!! ATTTTCATTTCCCAAATGTT+AGG - Chr4:56078260-56078279 MsG0480021265.01.T01:three_prime_UTR 25.0%
!! GTTTGAATAAAGATTAGTTG+TGG - Chr4:56079975-56079994 MsG0480021265.01.T01:intron 25.0%
!! TATTTGTAGTCAGAGTATTA+AGG - Chr4:56080719-56080738 MsG0480021265.01.T01:intron 25.0%
!! TATTTTCTGTAACTGCATAT+TGG + Chr4:56080748-56080767 None:intergenic 25.0%
!! TGGAAGATTTGTTTGAATTT+GGG - Chr4:56079074-56079093 MsG0480021265.01.T01:intron 25.0%
!! TTGATCGGTAAAATATTTTC+TGG + Chr4:56079333-56079352 None:intergenic 25.0%
!! TTTTCATTTCCCAAATGTTA+GGG - Chr4:56078261-56078280 MsG0480021265.01.T01:three_prime_UTR 25.0%
!!! CAGGCACATTTTTTTATATA+TGG + Chr4:56079763-56079782 None:intergenic 25.0%
!!! TTAAGGGTTTTTTATCTTCA+AGG - Chr4:56078785-56078804 MsG0480021265.01.T01:intron 25.0%
!!! TTGTTCACTAAAGTTTTGTA+TGG - Chr4:56080513-56080532 MsG0480021265.01.T01:intron 25.0%
AAATATGAGAGTGAATTGGA+AGG + Chr4:56078237-56078256 None:intergenic 30.0%
AAGAAATACCAGAAGTAAAC+GGG - Chr4:56079542-56079561 MsG0480021265.01.T01:intron 30.0%
AAGCCAAATTCACAGATAAA+TGG + Chr4:56079185-56079204 None:intergenic 30.0%
AATTACCAGAACTTAAACTC+AGG + Chr4:56078968-56078987 None:intergenic 30.0%
ACAAGGTTTAGTTGTTACAT+AGG - Chr4:56079904-56079923 MsG0480021265.01.T01:intron 30.0%
AGACCATTTATCTGTGAATT+TGG - Chr4:56079179-56079198 MsG0480021265.01.T01:intron 30.0%
AGCTTGCAACAAAATTCAAA+TGG + Chr4:56079431-56079450 None:intergenic 30.0%
ATGACGAAACAAAGTGAAAA+CGG + Chr4:56078363-56078382 None:intergenic 30.0%
ATTCTTATTATCATTGCTGC+AGG - Chr4:56080262-56080281 MsG0480021265.01.T01:CDS 30.0%
CAAGAAATACCAGAAGTAAA+CGG - Chr4:56079541-56079560 MsG0480021265.01.T01:intron 30.0%
CTATTTATTTAGCGACATTG+TGG - Chr4:56080032-56080051 MsG0480021265.01.T01:CDS 30.0%
GAATACGAAATCAAGATCTA+GGG + Chr4:56078318-56078337 None:intergenic 30.0%
GATTGTACTATCATGATTTC+AGG - Chr4:56080887-56080906 MsG0480021265.01.T01:five_prime_UTR 30.0%
GCAAAGCTTATACTATCAAA+TGG + Chr4:56079252-56079271 None:intergenic 30.0%
GTCGTAGGGTAAATAATTAA+AGG + Chr4:56080486-56080505 None:intergenic 30.0%
TACATTGTGTCTTATGGATT+GGG - Chr4:56078812-56078831 MsG0480021265.01.T01:intron 30.0%
TATTAAGAAAGCCTTTGTGA+AGG + Chr4:56080292-56080311 None:intergenic 30.0%
TGAACAAACATATAGTCGTA+GGG + Chr4:56080500-56080519 None:intergenic 30.0%
TGAATACGAAATCAAGATCT+AGG + Chr4:56078319-56078338 None:intergenic 30.0%
TGACATTTACACTCTTGATT+TGG - Chr4:56078530-56078549 MsG0480021265.01.T01:CDS 30.0%
TGGAAAATATGAGAGTGAAT+TGG + Chr4:56078241-56078260 None:intergenic 30.0%
TGTAACTATGCAATAATCAG+TGG - Chr4:56079401-56079420 MsG0480021265.01.T01:intron 30.0%
TTACATTGTGTCTTATGGAT+TGG - Chr4:56078811-56078830 MsG0480021265.01.T01:intron 30.0%
TTGCAACAAAATTCAAATGG+TGG + Chr4:56079428-56079447 None:intergenic 30.0%
TTGGAATAGATTCATCGATA+AGG - Chr4:56080003-56080022 MsG0480021265.01.T01:intron 30.0%
! AAGATTAGTTGTGGCATTAT+TGG - Chr4:56079984-56080003 MsG0480021265.01.T01:intron 30.0%
! ATTCTACTTATAGGTGGATT+GGG - Chr4:56078735-56078754 MsG0480021265.01.T01:intron 30.0%
! GAAGCTATTTCAGCATTATT+TGG - Chr4:56078697-56078716 MsG0480021265.01.T01:CDS 30.0%
! GGATTTTACATTGTGTCTTA+TGG - Chr4:56078806-56078825 MsG0480021265.01.T01:intron 30.0%
! TCTAAAACTGAATCAAACAC+TGG + Chr4:56080840-56080859 None:intergenic 30.0%
!! ATATTTTCTGGAAGAGCAAA+TGG + Chr4:56079321-56079340 None:intergenic 30.0%
!! ATCAATCAATTTTGCTGTGT+TGG - Chr4:56079722-56079741 MsG0480021265.01.T01:intron 30.0%
!! ATCTCAAAGTTAGGTTTTGT+GGG - Chr4:56080669-56080688 MsG0480021265.01.T01:intron 30.0%
!! GTTATTGGAAACTGATTTTG+TGG - Chr4:56079943-56079962 MsG0480021265.01.T01:intron 30.0%
!! TAACTTTGGGATCATTTGAA+AGG - Chr4:56080203-56080222 MsG0480021265.01.T01:CDS 30.0%
!! TTTGTTATTGCGTCCATATA+TGG - Chr4:56078761-56078780 MsG0480021265.01.T01:intron 30.0%
AAAATGCGATTAGGAAAGCA+AGG + Chr4:56079569-56079588 None:intergenic 35.0%
AAGAGCAAATGGAAATACTG+AGG + Chr4:56079310-56079329 None:intergenic 35.0%
AATAAACGGCTTGAACTCAT+CGG + Chr4:56078936-56078955 None:intergenic 35.0%
AATCGGAAAACCCTAACATT+TGG + Chr4:56078274-56078293 None:intergenic 35.0%
ACAGTTGAACAGAATAACGA+AGG + Chr4:56079793-56079812 None:intergenic 35.0%
ACTGGTACTTCGATATTCTT+CGG - Chr4:56079600-56079619 MsG0480021265.01.T01:intron 35.0%
AGAACACAGATCTCAAAGTT+AGG - Chr4:56080660-56080679 MsG0480021265.01.T01:intron 35.0%
AGATATATTCCGTTCAGCTT+GGG - Chr4:56080435-56080454 MsG0480021265.01.T01:intron 35.0%
ATCCACCTATAAGTAGAATG+TGG + Chr4:56078734-56078753 None:intergenic 35.0%
ATCGGAAAACCCTAACATTT+GGG + Chr4:56078273-56078292 None:intergenic 35.0%
ATTTGATCATGTGTGCGATT+TGG + Chr4:56080413-56080432 None:intergenic 35.0%
CAGATTGTGAATTGAATGGA+AGG - Chr4:56078611-56078630 MsG0480021265.01.T01:CDS 35.0%
CAGTTGAACAGAATAACGAA+GGG + Chr4:56079792-56079811 None:intergenic 35.0%
CATAGTTACATTGCATGCAA+TGG + Chr4:56079391-56079410 None:intergenic 35.0%
CATTCTACTTATAGGTGGAT+TGG - Chr4:56078734-56078753 MsG0480021265.01.T01:intron 35.0%
GAAAAATCTTGCAACCATTG+TGG + Chr4:56078677-56078696 None:intergenic 35.0%
GAATAACGAAGGGAAATTTC+AGG + Chr4:56079782-56079801 None:intergenic 35.0%
GATCAATGGAAAATGCGATT+AGG + Chr4:56079578-56079597 None:intergenic 35.0%
GATTCACGAGAATATGAAAG+AGG + Chr4:56079705-56079724 None:intergenic 35.0%
GTGAACAAACATATAGTCGT+AGG + Chr4:56080501-56080520 None:intergenic 35.0%
TAATTCAACTTCCCTGAAAG+AGG - Chr4:56080101-56080120 MsG0480021265.01.T01:CDS 35.0%
TACATGCTGAATCTGTTGAT+TGG - Chr4:56078839-56078858 MsG0480021265.01.T01:intron 35.0%
TATTTGATGTTCCTGTCTTC+TGG - Chr4:56080337-56080356 MsG0480021265.01.T01:CDS 35.0%
TGCGTCCATATATGGTTTAA+GGG - Chr4:56078769-56078788 MsG0480021265.01.T01:intron 35.0%
TTGCGTCCATATATGGTTTA+AGG - Chr4:56078768-56078787 MsG0480021265.01.T01:intron 35.0%
TTGGGATTCATTGCTAGTAT+CGG - Chr4:56079092-56079111 MsG0480021265.01.T01:intron 35.0%
! AAGCCAACGTGTTTAACTTT+GGG - Chr4:56080190-56080209 MsG0480021265.01.T01:CDS 35.0%
! AATGTTTCCAATTTTGTCGG+TGG - Chr4:56079509-56079528 MsG0480021265.01.T01:intron 35.0%
! ACATGCTGAATCTGTTGATT+GGG - Chr4:56078840-56078859 MsG0480021265.01.T01:intron 35.0%
! GATCTCAAAGTTAGGTTTTG+TGG - Chr4:56080668-56080687 MsG0480021265.01.T01:intron 35.0%
! GCATTTTCCATTGATCAAAC+TGG - Chr4:56079582-56079601 MsG0480021265.01.T01:intron 35.0%
! TAAGCCAACGTGTTTAACTT+TGG - Chr4:56080189-56080208 MsG0480021265.01.T01:CDS 35.0%
! TGTGTTTTCAATGCATCTCT+TGG - Chr4:56079147-56079166 MsG0480021265.01.T01:intron 35.0%
! TTTTCAATGCATCTCTTGGA+CGG - Chr4:56079151-56079170 MsG0480021265.01.T01:intron 35.0%
!! ATGTACACTTATGACTTGCT+TGG - Chr4:56080165-56080184 MsG0480021265.01.T01:CDS 35.0%
!! TCTCAAAGTTAGGTTTTGTG+GGG - Chr4:56080670-56080689 MsG0480021265.01.T01:intron 35.0%
!! TGTACACTTATGACTTGCTT+GGG - Chr4:56080166-56080185 MsG0480021265.01.T01:CDS 35.0%
AAATCTTGCAACCATTGTGG+CGG + Chr4:56078674-56078693 None:intergenic 40.0%
AATTGAATGGAAGGAACTGG+AGG - Chr4:56078620-56078639 MsG0480021265.01.T01:CDS 40.0%
ACCCAGTAGCAGAGTAATAT+TGG + Chr4:56080363-56080382 None:intergenic 40.0%
AGTAATATTGGCCAGAAGAC+AGG + Chr4:56080351-56080370 None:intergenic 40.0%
AGTTCAAGCCGTTTATTCGT+CGG - Chr4:56078939-56078958 MsG0480021265.01.T01:intron 40.0%
CACCACATTCTACTTATAGG+TGG - Chr4:56078729-56078748 MsG0480021265.01.T01:intron 40.0%
CAGATATATTCCGTTCAGCT+TGG - Chr4:56080434-56080453 MsG0480021265.01.T01:intron 40.0%
CCACACCACATTCTACTTAT+AGG - Chr4:56078726-56078745 MsG0480021265.01.T01:intron 40.0%
CCTATAAGTAGAATGTGGTG+TGG + Chr4:56078729-56078748 None:intergenic 40.0%
CCTGAACAGGAGAAAAATCA+AGG + Chr4:56080928-56080947 None:intergenic 40.0%
GATCCCAAAGTTAAACACGT+TGG + Chr4:56080196-56080215 None:intergenic 40.0%
GCAATAGTCATATCCTGAAC+AGG + Chr4:56080941-56080960 None:intergenic 40.0%
GCCAATATTACTCTGCTACT+GGG - Chr4:56080359-56080378 MsG0480021265.01.T01:CDS 40.0%
GTGAATTGAATGGAAGGAAC+TGG - Chr4:56078617-56078636 MsG0480021265.01.T01:CDS 40.0%
TCCATTTCCACCGACAAAAT+TGG + Chr4:56079519-56079538 None:intergenic 40.0%
TCTTATGCAAGTAGTAGCAG+TGG - Chr4:56080598-56080617 MsG0480021265.01.T01:intron 40.0%
TGGGATTCATTGCTAGTATC+GGG - Chr4:56079093-56079112 MsG0480021265.01.T01:intron 40.0%
! CCTTGATTTTTCTCCTGTTC+AGG - Chr4:56080925-56080944 MsG0480021265.01.T01:five_prime_UTR 40.0%
! CGAAGTACCAGTTTGATCAA+TGG + Chr4:56079592-56079611 None:intergenic 40.0%
! TCCAATTTTGTCGGTGGAAA+TGG - Chr4:56079515-56079534 MsG0480021265.01.T01:intron 40.0%
! TGAATTTTGTTGCAAGCTGC+TGG - Chr4:56079433-56079452 MsG0480021265.01.T01:intron 40.0%
! TGACACAGCAAAGCTTTAGT+CGG + Chr4:56080632-56080651 None:intergenic 40.0%
!! TTAGGTTTTGTGGGGATTCA+GGG - Chr4:56080678-56080697 MsG0480021265.01.T01:intron 40.0%
AACAAAGGTCCACCATCAGA+AGG + Chr4:56078899-56078918 None:intergenic 45.0%
AACTCATCGGAGCCTTTAGT+AGG + Chr4:56078923-56078942 None:intergenic 45.0%
ACCAGAAGACATGCTAATGC+TGG - Chr4:56080789-56080808 MsG0480021265.01.T01:intron 45.0%
ACCAGCATTAGCATGTCTTC+TGG + Chr4:56080793-56080812 None:intergenic 45.0%
ATAAGCTTTGCGTGCTTCAG+AGG - Chr4:56079261-56079280 MsG0480021265.01.T01:intron 45.0%
CTGCAGGTATTCCTTCACAA+AGG - Chr4:56080278-56080297 MsG0480021265.01.T01:CDS 45.0%
GAGCCTTTAGTAGGCAACAA+AGG + Chr4:56078914-56078933 None:intergenic 45.0%
GATTGGGTTTCTGTCTCCTT+TGG - Chr4:56078856-56078875 MsG0480021265.01.T01:intron 45.0%
GGACCTTTGTTGCCTACTAA+AGG - Chr4:56078908-56078927 MsG0480021265.01.T01:intron 45.0%
GGCCAATATTACTCTGCTAC+TGG - Chr4:56080358-56080377 MsG0480021265.01.T01:CDS 45.0%
GTTACATTGCATGCAATGGC+AGG + Chr4:56079387-56079406 None:intergenic 45.0%
TATAGCACCAGCCTCTTTCA+GGG + Chr4:56080115-56080134 None:intergenic 45.0%
TCAGGAAGCCGACGAATAAA+CGG + Chr4:56078950-56078969 None:intergenic 45.0%
! CGATTTGGCGTCTCATTGTA+AGG + Chr4:56080398-56080417 None:intergenic 45.0%
! GGCTTCCTGAGTTTAAGTTC+TGG - Chr4:56078960-56078979 MsG0480021265.01.T01:intron 45.0%
!! GTTAGGTTTTGTGGGGATTC+AGG - Chr4:56080677-56080696 MsG0480021265.01.T01:intron 45.0%
ACATTGCATGCAATGGCAGG+TGG + Chr4:56079384-56079403 None:intergenic 50.0%
ACCTGCTTTCCCAAGCTGAA+CGG + Chr4:56080447-56080466 None:intergenic 50.0%
AGAAGAAGAGCTGACCTGTG+AGG + Chr4:56078201-56078220 None:intergenic 50.0%
AGCAAGGACCCGTTTACTTC+TGG + Chr4:56079553-56079572 None:intergenic 50.0%
AGGTGGAGAAGGAAGAGGAT+TGG + Chr4:56078169-56078188 None:intergenic 50.0%
CATTGCATGCAATGGCAGGT+GGG + Chr4:56079383-56079402 None:intergenic 50.0%
GGAGAAGGAAGAGGATTGGA+GGG + Chr4:56078165-56078184 None:intergenic 50.0%
GTATAGCACCAGCCTCTTTC+AGG + Chr4:56080116-56080135 None:intergenic 50.0%
TCAACTTCCCTGAAAGAGGC+TGG - Chr4:56080105-56080124 MsG0480021265.01.T01:CDS 50.0%
TCCAGCTCAGGATCAACCAA+AGG + Chr4:56078875-56078894 None:intergenic 50.0%
TCCTTTGGTTGATCCTGAGC+TGG - Chr4:56078871-56078890 MsG0480021265.01.T01:intron 50.0%
TGGAGAAGGAAGAGGATTGG+AGG + Chr4:56078166-56078185 None:intergenic 50.0%
! TTGTGGGGATTCAGGGTACT+TGG - Chr4:56080685-56080704 MsG0480021265.01.T01:intron 50.0%
AGAAGAGCTGACCTGTGAGG+AGG + Chr4:56078198-56078217 None:intergenic 55.0%
ATCGGAGGTGGAGAAGGAAG+AGG + Chr4:56078174-56078193 None:intergenic 55.0%
CGTAGCTGCGGACAAGTTCA+TGG - Chr4:56079464-56079483 MsG0480021265.01.T01:intron 55.0%
CTGGTCATTGCACGTAGCTG+CGG - Chr4:56079452-56079471 MsG0480021265.01.T01:intron 55.0%
GAAGAGCTGACCTGTGAGGA+GGG + Chr4:56078197-56078216 None:intergenic 55.0%
TCCGTTCAGCTTGGGAAAGC+AGG - Chr4:56080443-56080462 MsG0480021265.01.T01:intron 55.0%
CCATCAGAAGGCTCCAGCTC+AGG + Chr4:56078887-56078906 None:intergenic 60.0%
CCTGAGCTGGAGCCTTCTGA+TGG - Chr4:56078884-56078903 MsG0480021265.01.T01:intron 60.0%
GAGCTGGAGCCTTCTGATGG+TGG - Chr4:56078887-56078906 MsG0480021265.01.T01:intron 60.0%
GCTGACCTGTGAGGAGGGAT+CGG + Chr4:56078192-56078211 None:intergenic 60.0%
CACCTCCGATCCCTCCTCAC+AGG - Chr4:56078184-56078203 MsG0480021265.01.T01:three_prime_UTR 65.0%
CTGCTGCTGCACCGCCACAA+TGG - Chr4:56078660-56078679 MsG0480021265.01.T01:CDS 65.0%
CTGTGAGGAGGGATCGGAGG+TGG + Chr4:56078186-56078205 None:intergenic 65.0%
GACCTGTGAGGAGGGATCGG+AGG + Chr4:56078189-56078208 None:intergenic 65.0%
GGAGGGATCGGAGGTGGAGA+AGG + Chr4:56078180-56078199 None:intergenic 65.0%
CGGTGCAGCAGCAGTCGCAG+AGG + Chr4:56078654-56078673 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56078034 56080972 56078034 ID=MsG0480021265.01;Name=MsG0480021265.01
Chr4 mRNA 56078034 56080972 56078034 ID=MsG0480021265.01.T01;Parent=MsG0480021265.01;Name=MsG0480021265.01.T01;_AED=0.50;_eAED=0.51;_QI=184|0.66|0.75|1|0|0.25|4|336|217
Chr4 exon 56078034 56078426 56078034 ID=MsG0480021265.01.T01:exon:6371;Parent=MsG0480021265.01.T01
Chr4 exon 56078482 56078722 56078482 ID=MsG0480021265.01.T01:exon:6370;Parent=MsG0480021265.01.T01
Chr4 exon 56080025 56080392 56080025 ID=MsG0480021265.01.T01:exon:6369;Parent=MsG0480021265.01.T01
Chr4 exon 56080801 56080972 56080801 ID=MsG0480021265.01.T01:exon:6368;Parent=MsG0480021265.01.T01
Chr4 five_prime_UTR 56080801 56080972 56080801 ID=MsG0480021265.01.T01:five_prime_utr;Parent=MsG0480021265.01.T01
Chr4 five_prime_UTR 56080381 56080392 56080381 ID=MsG0480021265.01.T01:five_prime_utr;Parent=MsG0480021265.01.T01
Chr4 CDS 56080025 56080380 56080025 ID=MsG0480021265.01.T01:cds;Parent=MsG0480021265.01.T01
Chr4 CDS 56078482 56078722 56078482 ID=MsG0480021265.01.T01:cds;Parent=MsG0480021265.01.T01
Chr4 CDS 56078370 56078426 56078370 ID=MsG0480021265.01.T01:cds;Parent=MsG0480021265.01.T01
Chr4 three_prime_UTR 56078034 56078369 56078034 ID=MsG0480021265.01.T01:three_prime_utr;Parent=MsG0480021265.01.T01
Gene Sequence

>MsG0480021265.01.T01

ATGGAAGGAACTGGAGGCAGCAGTGCCTCTGCGACTGCTGCTGCACCGCCACAATGGTTGCAAGATTTTTCGAAGCTATTTCAGCATTATTTGGATAAATCCACACCACATTCTACTTATAGGTGGATTGGGACTTTTGTTATTGCGTCCATATATGGTTTAAGGGTTTTTTATCTTCAAGGATTTTACATTGTGTCTTATGGATTGGGAATTTACATGCTGAATCTGTTGATTGGGTTTCTGTCTCCTTTGGTTGATCCTGAGCTGGAGCCTTCTGATGGTGGACCTTTGTTGCCTACTAAAGGCTCCGATGAGTTCAAGCCGTTTATTCGTCGGCTTCCTGAGTTTAAGTTCTGGTATTCCTTCACAAAGGCTTTCTTAATAGCATTCTTGATGACTTTCTTTTCTGTATTTGATGTTCCTGTCTTCTGGCCAATATTACTCTGCTACTGGGTTGTTCTGTTTGTCCTTACAATGAGACGCCAAATCGCACACATGATCAAATACAGATATATTCCGTTCAGCTTGGGAAAGCAGGTTTTTATGCTTAGATTGCCTTTAATTATTTACCCTACGACTATATGTTTGTTCACTAAAAAATATGGTGGCAAGAAATCTTATGCAAGTAGTAGCAGTGGCTCTCGTGCCGACTAA
Protein sequence

>MsG0480021265.01.T01

MEGTGGSSASATAAAPPQWLQDFSKLFQHYLDKSTPHSTYRWIGTFVIASIYGLRVFYLQGFYIVSYGLGIYMLNLLIGFLSPLVDPELEPSDGGPLLPTKGSDEFKPFIRRLPEFKFWYSFTKAFLIAFLMTFFSVFDVPVFWPILLCYWVVLFVLTMRRQIAHMIKYRYIPFSLGKQVFMLRLPLIIYPTTICLFTKKYGGKKSYASSSSGSRAD*