AlfalfaGEDB | MsG0180000857.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000857.01

KEGG: K12586
GO: -
PFAM: PF01138

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000857.01.T01	KAG5016694.1	80.46	348	39	4	1	341	1	326	0	550

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000857.01.T01	Q96B26	31.056	322	169	5	20	339	4	274	2.33E-51	174

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000857.01.T01	G7I253	87.977	341	4	1	1	341	1	304	0.0	596

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000857.01	MsG0280011419.01	0.803415	3.524207e-49	1.769939e-46
MsG0180000857.01	MsG0380012279.01	0.801401	9.161298e-49	4.370926e-46
MsG0180000857.01	MsG0480023959.01	0.802061	6.706886e-49	3.253966e-46
MsG0180000857.01	MsG0880047371.01	0.803704	3.070778e-49	1.553650e-46

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PPI

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Gene1	Gene2	Type
MsG0780038909.01	MsG0180000857.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000857.01.T01	MTR_1g007060	87.977	341	4	1	1	341	1	304	0.0	596
MsG0180000857.01.T01	MTR_1g007060	86.943	314	4	1	1	314	1	277	0.0	542
MsG0180000857.01.T01	MTR_1g063090	26.168	321	180	9	28	341	17	287	1.26e-24	102
MsG0180000857.01.T01	MTR_8g077430	27.726	321	176	6	29	341	11	283	3.59e-23	97.8
MsG0180000857.01.T01	MTR_2g019210	25.714	315	178	9	28	339	17	278	7.98e-23	98.2
MsG0180000857.01.T01	MTR_1g031820	27.445	317	184	6	28	341	407	680	8.41e-23	99.8
MsG0180000857.01.T01	MTR_2g019210	25.714	315	178	9	28	339	17	278	4.26e-22	97.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000857.01.T01	AT1G60080	63.582	335	79	3	1	331	1	296	5.95e-140	399
MsG0180000857.01.T01	AT3G07750	27.187	320	177	6	28	339	9	280	1.53e-23	99.0
MsG0180000857.01.T01	AT3G07750	27.187	320	177	6	28	339	9	280	1.53e-23	99.0
MsG0180000857.01.T01	AT3G60500	24.606	317	186	8	28	341	17	283	4.66e-17	81.6
MsG0180000857.01.T01	AT3G60500	24.921	317	185	9	28	341	17	283	1.89e-16	80.5
MsG0180000857.01.T01	AT3G60500	24.921	317	185	9	28	341	17	283	1.89e-16	80.5
MsG0180000857.01.T01	AT3G60500	24.921	317	185	9	28	341	17	283	1.89e-16	80.5
MsG0180000857.01.T01	AT3G12990	25.000	300	170	9	28	323	17	265	5.42e-16	77.8
MsG0180000857.01.T01	AT3G12990	25.000	300	170	9	28	323	17	265	7.26e-16	77.4
MsG0180000857.01.T01	AT3G12990	25.000	300	170	9	28	323	17	265	7.26e-16	77.4

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Find 100 sgRNAs with CRISPR-Local

Find 454 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCCATGTGACAATCGTTC+TGG	0.387086	1:-12178239	MsG0180000857.01.T01:CDS
AACTTGCCTTGTAATGGTGT+CGG	0.554769	1:+12182119	None:intergenic
AAGGAATCAACGAGTGGGGA+GGG	0.529973	1:+12183888	None:intergenic
AATGCAGGATATCTATTGTT+TGG	0.323709	1:-12181409	MsG0180000857.01.T01:intron
ACAACCGGCTCTGCTTCAGC+AGG	0.520816	1:+12182156	None:intergenic
ACAATCGGAGAACAAATCGG+AGG	0.723892	1:+12182192	None:intergenic
ACAATCGTTCTGGATACATC+TGG	0.510136	1:-12178229	MsG0180000857.01.T01:CDS
ACAGTTTCGTATTTGCTTGA+CGG	0.489705	1:+12183847	None:intergenic
ACCCTCGTCTGGTGACTCCA+AGG	0.603409	1:+12182343	None:intergenic
ACGGAGAAGGAATCAACGAG+TGG	0.655448	1:+12183882	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGTAAGAATCTAGAGAG+AGG	-	Chr1:12180223-12180242	MsG0180000857.01.T01:intron	30.0%
AAACAATGACAGTTCACATT+TGG	-	Chr1:12181564-12181583	MsG0180000857.01.T01:intron	30.0%
AAACACAAGTACACAACAAA+AGG	+	Chr1:12182323-12182342	None:intergenic	30.0%
AAAGTAAGAATCTAGAGAGA+GGG	-	Chr1:12180224-12180243	MsG0180000857.01.T01:intron	30.0%
AAATCAATACGGTAATACGA+CGG	+	Chr1:12182641-12182660	None:intergenic	30.0%
AACAATGACAGTTCACATTT+GGG	-	Chr1:12181565-12181584	MsG0180000857.01.T01:CDS	30.0%
AACTAAAACGTGAGTGTTTA+GGG	+	Chr1:12182164-12182183	None:intergenic	30.0%
AAGAGAGTTGTGTTCTTTAA+CGG	+	Chr1:12181377-12181396	None:intergenic	30.0%
AATGAGATCATCAGTTAAAG+AGG	-	Chr1:12181503-12181522	MsG0180000857.01.T01:intron	30.0%
AATGCAGGATATCTATTGTT+TGG	-	Chr1:12179721-12179740	MsG0180000857.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	12177218	12183934	12177218	ID=MsG0180000857.01;Name=MsG0180000857.01
Chr1	mRNA	12177218	12183934	12177218	ID=MsG0180000857.01.T01;Parent=MsG0180000857.01;Name=MsG0180000857.01.T01;_AED=0.45;_eAED=0.49;_QI=123\|0.75\|0.77\|1\|0.87\|0.88\|9\|168\|341
Chr1	exon	12183646	12183934	12183646	ID=MsG0180000857.01.T01:exon:326;Parent=MsG0180000857.01.T01
Chr1	exon	12182520	12182569	12182520	ID=MsG0180000857.01.T01:exon:327;Parent=MsG0180000857.01.T01
Chr1	exon	12182336	12182405	12182336	ID=MsG0180000857.01.T01:exon:328;Parent=MsG0180000857.01.T01
Chr1	exon	12182067	12182231	12182067	ID=MsG0180000857.01.T01:exon:329;Parent=MsG0180000857.01.T01
Chr1	exon	12181732	12181783	12181732	ID=MsG0180000857.01.T01:exon:330;Parent=MsG0180000857.01.T01
Chr1	exon	12181565	12181631	12181565	ID=MsG0180000857.01.T01:exon:331;Parent=MsG0180000857.01.T01
Chr1	exon	12181349	12181424	12181349	ID=MsG0180000857.01.T01:exon:332;Parent=MsG0180000857.01.T01
Chr1	exon	12178168	12178463	12178168	ID=MsG0180000857.01.T01:exon:333;Parent=MsG0180000857.01.T01
Chr1	exon	12177218	12177469	12177218	ID=MsG0180000857.01.T01:exon:334;Parent=MsG0180000857.01.T01
Chr1	five_prime_UTR	12183812	12183934	12183812	ID=MsG0180000857.01.T01:five_prime_utr;Parent=MsG0180000857.01.T01
Chr1	CDS	12183646	12183811	12183646	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12182520	12182569	12182520	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12182336	12182405	12182336	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12182067	12182231	12182067	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12181732	12181783	12181732	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12181565	12181631	12181565	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12181349	12181424	12181349	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12178168	12178463	12178168	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	CDS	12177386	12177469	12177386	ID=MsG0180000857.01.T01:cds;Parent=MsG0180000857.01.T01
Chr1	three_prime_UTR	12177218	12177385	12177218	ID=MsG0180000857.01.T01:three_prime_utr;Parent=MsG0180000857.01.T01

Gene Sequence

>MsG0180000857.01.T01

ATGAGTGTTCCAAATGCTGCTGCAACGGAGGATTTGTCGTCTGAAATGGAAGTCGATGCGTTTCGACGCCTTTTCCCGCTTCGATATTTCGAACGTCATCTTGCTGAATCTATTCGTTCTGATGGTAGACCGCTTAGAGAAGCTAGAGATACCAGTATTTTTCTTGGTGCTGTTGCATCTGCTAATGGGTCAGCTCTTGTGAAGATTGGATCCACGACTATGTTGACTGCCATTAAAATGGAAGTTATGACTCCTTCCTTGGAGTCACCAGACGAGGGTTGTTTAGCCATAGACTTCCATATGCCTCCGATTTGTTCTCCGATTGTTAGGCCTGGCAGGCCTGCTGAAGCAGAGCCGGTTGTGTCAAAGCAGTTGTCCGACACCATTACAAGGCAAGTTTTAATCTTTCATGTTGCTTTGTTAGGTTCAATTATTTCAGATTATGCAAGAACTTTGAAGGACATTATATTTGTGTTGGTCTATGTTATAATTTTGGGTAATCGTTCTGGAATGATTGATTTGAGAGAGTTGTCCTTGGTTGGTGGAAAAGCTGCATGGATGGCTTACTTGGATATCTATTGTTTGGATGCTGACGGGTCTCTTTTTGATGCTGCTTTACTTTCTGCAGTTGCTGCTTTATCTCATTTGCAAATTCCTGCTGTAGCCATGAATGATGACGGCAAAATAGTACTCATGTCTGATGAAGATGGACAGAAGCAGACAAAGGAGCAAGTCAATAAGGAGAAGAGGAAGCTTACATTAAAAAGCATTCCATTATCATTAACATGCATACTTCACAAGAACTACATCTTGGCAGATCCTACTGCAGAAGAAGAATCCATAGTGGAAACCCATGTGACAATCGTTCTGGATACATCTGGTCAACTAATATCTCTTTACAAGCCTGGTGGGCCAGTTCTTGCCTATACTTCTGCTATCCAGGACTGTGTTGCCTTGACTAGGCAAAGAGTGAAGGAACTAAAGAGCTTCTTAGACAAAGAAAATTCTGCTATGGAGGTTGAGTAG

Protein sequence

>MsG0180000857.01.T01

MSVPNAAATEDLSSEMEVDAFRRLFPLRYFERHLAESIRSDGRPLREARDTSIFLGAVASANGSALVKIGSTTMLTAIKMEVMTPSLESPDEGCLAIDFHMPPICSPIVRPGRPAEAEPVVSKQLSDTITRQVLIFHVALLGSIISDYARTLKDIIFVLVYVIILGNRSGMIDLRELSLVGGKAAWMAYLDIYCLDADGSLFDAALLSAVAALSHLQIPAVAMNDDGKIVLMSDEDGQKQTKEQVNKEKRKLTLKSIPLSLTCILHKNYILADPTAEEESIVETHVTIVLDTSGQLISLYKPGGPVLAYTSAIQDCVALTRQRVKELKSFLDKENSAMEVE*