AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012279.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012279.01.T01 MTR_3g020660 100.000 159 0 0 41 199 1 159 1.32e-114 323
MsG0380012279.01.T01 MTR_4g071000 89.241 158 17 0 41 198 1 158 3.72e-101 289
MsG0380012279.01.T01 MTR_3g020520 95.833 120 5 0 80 199 407 526 3.61e-76 238
MsG0380012279.01.T01 MTR_3g020640 83.453 139 16 1 61 199 1000 1131 3.32e-68 225
MsG0380012279.01.T01 MTR_3g020650 82.353 85 15 0 78 162 1028 1112 1.07e-39 144
MsG0380012279.01.T02 MTR_3g020660 100.000 159 0 0 41 199 1 159 1.32e-114 323
MsG0380012279.01.T02 MTR_4g071000 89.241 158 17 0 41 198 1 158 3.72e-101 289
MsG0380012279.01.T02 MTR_3g020520 95.833 120 5 0 80 199 407 526 3.61e-76 238
MsG0380012279.01.T02 MTR_3g020640 83.453 139 16 1 61 199 1000 1131 3.32e-68 225
MsG0380012279.01.T02 MTR_3g020650 82.353 85 15 0 78 162 1028 1112 1.07e-39 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012279.01.T01 AT1G17880 85.430 151 20 2 41 190 1 150 7.75e-87 253
MsG0380012279.01.T01 AT1G73230 85.987 157 21 1 41 196 1 157 5.60e-84 246
MsG0380012279.01.T02 AT1G17880 85.430 151 20 2 41 190 1 150 7.75e-87 253
MsG0380012279.01.T02 AT1G73230 85.987 157 21 1 41 196 1 157 5.60e-84 246

Find 0 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTCATTTATATTTT+TGG - Chr3:14020547-14020566 MsG0380012279.01.T02:intron 10.0%
!! ATGATTGTGTAAAAAATATT+TGG - Chr3:14020468-14020487 MsG0380012279.01.T02:intron 15.0%
!! ATTATAATACAGTAATAAGA+AGG - Chr3:14020429-14020448 MsG0380012279.01.T02:intron 15.0%
!! TTTCCAAAATTAGTATAAAA+AGG + Chr3:14020654-14020673 None:intergenic 15.0%
!!! AATCTTATATTCTTATTTCT+TGG - Chr3:14021931-14021950 MsG0380012279.01.T02:intron 15.0%
!!! AATTGTTTTTTATTTGATTG+TGG - Chr3:14021069-14021088 MsG0380012279.01.T02:intron 15.0%
!!! TTATTTCTTGGAAAAATTTA+GGG - Chr3:14021943-14021962 MsG0380012279.01.T02:intron 15.0%
!! AAATTACTGACAAAAATTAC+AGG + Chr3:14020694-14020713 None:intergenic 20.0%
!!! CTTATTTCTTGGAAAAATTT+AGG - Chr3:14021942-14021961 MsG0380012279.01.T02:intron 20.0%
!!! GAACCTTTTTATACTAATTT+TGG - Chr3:14020648-14020667 MsG0380012279.01.T02:intron 20.0%
!!! GATTTCATTTATATTTTTGG+TGG - Chr3:14020550-14020569 MsG0380012279.01.T02:intron 20.0%
!!! TAGTTTTACGAAACAAAAAT+TGG + Chr3:14021029-14021048 None:intergenic 20.0%
!!! TGTTTGATAATGTATCATTA+AGG + Chr3:14021498-14021517 None:intergenic 20.0%
! ACTAACTGTTAAACTTGAAT+TGG - Chr3:14021381-14021400 MsG0380012279.01.T02:intron 25.0%
! ATGTAAATCACATACCTAAT+TGG + Chr3:14021875-14021894 None:intergenic 25.0%
! CGTGAATTAATATTACCTTT+GGG + Chr3:14021318-14021337 None:intergenic 25.0%
! GTGAATTAATATTACCTTTG+GGG + Chr3:14021317-14021336 None:intergenic 25.0%
! TCATTTATTCATCTGTACAA+AGG - Chr3:14020750-14020769 MsG0380012279.01.T02:intron 25.0%
! TCGTGAATTAATATTACCTT+TGG + Chr3:14021319-14021338 None:intergenic 25.0%
! TGAAATCAGATCAAAATCAA+AGG + Chr3:14021779-14021798 None:intergenic 25.0%
!! AAATTTTGAACATTCCAACA+TGG + Chr3:14021685-14021704 None:intergenic 25.0%
!! TTCAGATTTTCCAAATTATC+TGG + Chr3:14021969-14021988 None:intergenic 25.0%
AAAATGAGGAAAACAATGCA+TGG + Chr3:14021731-14021750 None:intergenic 30.0%
ATTGGAGTTTAAGAGATACA+TGG + Chr3:14021011-14021030 None:intergenic 30.0%
CATCAATGTTCAGAAATTGT+AGG - Chr3:14021631-14021650 MsG0380012279.01.T02:intron 30.0%
GAAGTCTCACAATTAAAATG+AGG + Chr3:14021745-14021764 None:intergenic 30.0%
GATTGCTAGTAGTACATTAA+TGG + Chr3:14020942-14020961 None:intergenic 30.0%
TATGTTTACACTGAGATATC+TGG - Chr3:14020369-14020388 MsG0380012279.01.T02:CDS 30.0%
TGAGGAAGTCAACATATTTA+AGG - Chr3:14021266-14021285 MsG0380012279.01.T02:intron 30.0%
TTGGATTCAGTCAATATGAT+AGG - Chr3:14020487-14020506 MsG0380012279.01.T02:intron 30.0%
! AAATTTAGGGCCAGATAATT+TGG - Chr3:14021956-14021975 MsG0380012279.01.T02:intron 30.0%
!! AAATATGTTGACTTCCTCAA+TGG + Chr3:14021265-14021284 None:intergenic 30.0%
!! AGCCTTCTTCTTTCTAAAAT+GGG + Chr3:14021166-14021185 None:intergenic 30.0%
!! ATATCTGGTTCTTTCTTTGA+TGG - Chr3:14020384-14020403 MsG0380012279.01.T02:CDS 30.0%
!! ATTTTGATCTGATTTCATGC+TGG - Chr3:14021782-14021801 MsG0380012279.01.T02:intron 30.0%
!! TATCTGGTTCTTTCTTTGAT+GGG - Chr3:14020385-14020404 MsG0380012279.01.T02:CDS 30.0%
!! TGTAGAAAAGTTGATGAAGA+TGG - Chr3:14020837-14020856 MsG0380012279.01.T02:intron 30.0%
ACCTAATTGGTGAATGATGT+TGG + Chr3:14021862-14021881 None:intergenic 35.0%
ATATGCTTACTCTTTGTCTG+AGG + Chr3:14021457-14021476 None:intergenic 35.0%
ATCTATTGCTGCCAATACTT+GGG - Chr3:14021417-14021436 MsG0380012279.01.T02:intron 35.0%
ATTCAGTTTCTAAACCCCAA+AGG - Chr3:14021300-14021319 MsG0380012279.01.T02:intron 35.0%
CCTAATTGGTGAATGATGTT+GGG + Chr3:14021861-14021880 None:intergenic 35.0%
CTAATTGGTGAATGATGTTG+GGG + Chr3:14021860-14021879 None:intergenic 35.0%
CTGCTTATCAGAGATAATAC+AGG + Chr3:14021097-14021116 None:intergenic 35.0%
TCAGTGTAAACATACACTGT+AGG + Chr3:14020363-14020382 None:intergenic 35.0%
! ATATCCTGCAACTCTGAAAA+AGG + Chr3:14021834-14021853 None:intergenic 35.0%
! CAGCCTTCTTCTTTCTAAAA+TGG + Chr3:14021167-14021186 None:intergenic 35.0%
! TATCCTGCAACTCTGAAAAA+GGG + Chr3:14021833-14021852 None:intergenic 35.0%
!! TGCCCATTTTAGAAAGAAGA+AGG - Chr3:14021161-14021180 MsG0380012279.01.T02:CDS 35.0%
ACATGGCACACATCATACAA+TGG + Chr3:14021668-14021687 None:intergenic 40.0%
CAACTGAAACCTTACCACTT+TGG - Chr3:14020977-14020996 MsG0380012279.01.T02:intron 40.0%
CATCTATTGCTGCCAATACT+TGG - Chr3:14021416-14021435 MsG0380012279.01.T02:intron 40.0%
CCCAACATCATTCACCAATT+AGG - Chr3:14021858-14021877 MsG0380012279.01.T02:intron 40.0%
CTGAAGAGAATTGGAGTGAA+TGG - Chr3:14021231-14021250 MsG0380012279.01.T02:intron 40.0%
GAGGAAAACAATGCATGGTA+TGG + Chr3:14021726-14021745 None:intergenic 40.0%
TTGTATGATGTGTGCCATGT+TGG - Chr3:14021668-14021687 MsG0380012279.01.T02:intron 40.0%
! CAAAGCACTCTGAAGAGAAT+TGG - Chr3:14021222-14021241 MsG0380012279.01.T02:intron 40.0%
!! GAAAAGTTGATGAAGATGGC+CGG - Chr3:14020841-14020860 MsG0380012279.01.T02:intron 40.0%
!!! TTTCCCTTTTTCAGAGTTGC+AGG - Chr3:14021827-14021846 MsG0380012279.01.T02:intron 40.0%
ACCGCTAACAACCCAAGTAT+TGG + Chr3:14021431-14021450 None:intergenic 45.0%
AGCTGAGCAATTTCAGAAGC+AGG - Chr3:14021995-14022014 MsG0380012279.01.T02:intron 45.0%
CTGTAGGAACCTATGAAGCA+CGG + Chr3:14020347-14020366 None:intergenic 45.0%
TCTGTTAGAACCGGTGGAAA+GGG - Chr3:14020865-14020884 MsG0380012279.01.T02:intron 45.0%
TGAAACCTTACCACTTTGGC+TGG - Chr3:14020981-14021000 MsG0380012279.01.T02:intron 45.0%
TTCTGTTAGAACCGGTGGAA+AGG - Chr3:14020864-14020883 MsG0380012279.01.T02:intron 45.0%
! GCCAATACTTGGGTTGTTAG+CGG - Chr3:14021427-14021446 MsG0380012279.01.T02:intron 45.0%
!! ATGTTGACTTCCTCAATGGC+AGG + Chr3:14021261-14021280 None:intergenic 45.0%
!! TGTTGACTTCCTCAATGGCA+GGG + Chr3:14021260-14021279 None:intergenic 45.0%
ACATGGATAGCCAGCCAAAG+TGG + Chr3:14020994-14021013 None:intergenic 50.0%
AGCAGTTTCAAAGGTCTCCC+CGG + Chr3:14022090-14022109 None:intergenic 50.0%
CGTCTGTAGTAGCTGTCTTG+TGG + Chr3:14021191-14021210 None:intergenic 50.0%
CTCCTCAGCAGCAGTTTCAA+AGG + Chr3:14022099-14022118 None:intergenic 50.0%
GAATGGTATCCCTGCCATTG+AGG - Chr3:14021248-14021267 MsG0380012279.01.T02:intron 50.0%
GATAGCCAGCCAAAGTGGTA+AGG + Chr3:14020989-14021008 None:intergenic 50.0%
GATTAGTAGTGTCCCGGTGT+TGG + Chr3:14020314-14020333 None:intergenic 50.0%
TATGAAGCACGGACACTCCT+CGG + Chr3:14020336-14020355 None:intergenic 50.0%
TCTGACTGTACCCTTTCCAC+CGG + Chr3:14020878-14020897 None:intergenic 50.0%
TGATGCAACTGCAGCACAAG+AGG - Chr3:14022034-14022053 MsG0380012279.01.T02:CDS 50.0%
! ATGGCCGGTTCTGTTAGAAC+CGG - Chr3:14020856-14020875 MsG0380012279.01.T02:intron 50.0%
! GTGGAAAGGGTACAGTCAGA+AGG - Chr3:14020878-14020897 MsG0380012279.01.T02:intron 50.0%
!! GACCTTTGAAACTGCTGCTG+AGG - Chr3:14022094-14022113 MsG0380012279.01.T02:CDS 50.0%
!! TCCACCGGTTCTAACAGAAC+CGG + Chr3:14020863-14020882 None:intergenic 50.0%
!! TCCTCGGATTAGTAGTGTCC+CGG + Chr3:14020320-14020339 None:intergenic 50.0%
AACTGCTGCTGAGGAGACCA+AGG - Chr3:14022103-14022122 MsG0380012279.01.T02:CDS 55.0%
ACCGGGACACTACTAATCCG+AGG - Chr3:14020316-14020335 MsG0380012279.01.T02:CDS 55.0%
CAGTTTCAAAGGTCTCCCCG+GGG + Chr3:14022088-14022107 None:intergenic 55.0%
CTGTACCAGATCTTGTCCCC+GGG - Chr3:14022069-14022088 MsG0380012279.01.T02:CDS 55.0%
GCAGTTTCAAAGGTCTCCCC+GGG + Chr3:14022089-14022108 None:intergenic 55.0%
GCTGTACCAGATCTTGTCCC+CGG - Chr3:14022068-14022087 MsG0380012279.01.T02:CDS 55.0%
GTTGCATCAGCTCCTGCTTC+AGG + Chr3:14022023-14022042 None:intergenic 55.0%
TGTACCAGATCTTGTCCCCG+GGG - Chr3:14022070-14022089 MsG0380012279.01.T02:CDS 55.0%
!! GAGGAGTGTCCGTGCTTCAT+AGG - Chr3:14020335-14020354 MsG0380012279.01.T02:CDS 55.0%
CAGAAGCAGGCTCCTGAAGC+AGG - Chr3:14022008-14022027 MsG0380012279.01.T02:intron 60.0%
! GCCGGTTCTGTTAGAACCGG+TGG - Chr3:14020859-14020878 MsG0380012279.01.T02:intron 60.0%
!! CGTCGGTGTCGTGTTGGTGT+CGG - Chr3:14020274-14020293 MsG0380012279.01.T02:CDS 60.0%
!! GTCGTGTTGGTGTCGGACAC+CGG - Chr3:14020281-14020300 MsG0380012279.01.T02:CDS 60.0%
GTCTCCCCGGGGACAAGATC+TGG + Chr3:14022077-14022096 None:intergenic 65.0%
ACCGGCGCGTGTCCAACACC+GGG - Chr3:14020299-14020318 MsG0380012279.01.T02:CDS 70.0%
CACCGGCGCGTGTCCAACAC+CGG - Chr3:14020298-14020317 MsG0380012279.01.T02:CDS 70.0%
! TCCCGGTGTTGGACACGCGC+CGG + Chr3:14020303-14020322 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 14020271 14022130 14020271 ID=MsG0380012279.01;Name=MsG0380012279.01
Chr3 mRNA 14020271 14022130 14020271 ID=MsG0380012279.01.T01;Parent=MsG0380012279.01;Name=MsG0380012279.01.T01;_AED=0.47;_eAED=0.54;_QI=0|0.83|0.85|1|0.83|0.71|7|0|199
Chr3 CDS 14022011 14022130 14022011 ID=MsG0380012279.01.T01:cds;Parent=MsG0380012279.01.T01
Chr3 CDS 14021502 14021569 14021502 ID=MsG0380012279.01.T01:cds;Parent=MsG0380012279.01.T01
Chr3 CDS 14021080 14021228 14021080 ID=MsG0380012279.01.T01:cds;Parent=MsG0380012279.01.T01
Chr3 CDS 14020935 14020991 14020935 ID=MsG0380012279.01.T01:cds;Parent=MsG0380012279.01.T01
Chr3 CDS 14020522 14020560 14020522 ID=MsG0380012279.01.T01:cds;Parent=MsG0380012279.01.T01
Chr3 CDS 14020271 14020437 14020271 ID=MsG0380012279.01.T01:cds;Parent=MsG0380012279.01.T01
Chr3 mRNA 14020271 14022130 14020271 ID=MsG0380012279.01.T02;Parent=MsG0380012279.01;Name=MsG0380012279.01.T02;_AED=0.47;_eAED=0.55;_QI=0|0.8|0.83|1|1|1|6|0|199
Chr3 CDS 14022011 14022130 14022011 ID=MsG0380012279.01.T02:cds;Parent=MsG0380012279.01.T02
Chr3 CDS 14021502 14021569 14021502 ID=MsG0380012279.01.T02:cds;Parent=MsG0380012279.01.T02
Chr3 CDS 14021080 14021228 14021080 ID=MsG0380012279.01.T02:cds;Parent=MsG0380012279.01.T02
Chr3 CDS 14020935 14020991 14020935 ID=MsG0380012279.01.T02:cds;Parent=MsG0380012279.01.T02
Chr3 CDS 14020522 14020560 14020522 ID=MsG0380012279.01.T02:cds;Parent=MsG0380012279.01.T02
Chr3 CDS 14020271 14020437 14020271 ID=MsG0380012279.01.T02:cds;Parent=MsG0380012279.01.T02
Gene Sequence

>MsG0380012279.01.T01

ATGCGTCGGTGTCGTGTTGGTGTCGGACACCGGCGCGTGTCCAACACCGGGACACTACTAATCCGAGGAGTGTCCGTGCTTCATAGGTTCCTACAGTGTATGTTTACACTGAGATATCTGATGAATGTAGAAAAGTTGATGAAGATGGCCGGTTCTGTTAGAACCGGTGGAAAGGGTACAGTCAGAAGAAAGAAGAAGGCTGTCCACAAGACAGCTACTACAGACGACAAAAGACTTCAAAGCACTCTGAAGAGAATTGGAGTGAATGGTATCCCTGCCATTGAGGAAGTCAACATATTTAAGGATGATGTAGTTATTCAGTTTCTAAACCCCAAAGTACAAGCATCTATTGCTGCCAATACTTGGGTTGTTAGCGGTTCTCCTCAGACAAAGAAGTTGCAGGATATTCTCCCCAACATCATTCACCAATTAGGGCCAGATAATTTGGAAAATCTGAAGAAGCTAGCTGAGCAATTTCAGAAGCAGGCTCCTGAAGCAGGAGCTGATGCAACTGCAGCACAAGAGGATGATGATGATGCTGTACCAGATCTTGTCCCCGGGGAGACCTTTGAAACTGCTGCTGAGGAGACCAAGGCTTGA

>MsG0380012279.01.T02

ATGCGTCGGTGTCGTGTTGGTGTCGGACACCGGCGCGTGTCCAACACCGGGACACTACTAATCCGAGGAGTGTCCGTGCTTCATAGGTTCCTACAGTGTATGTTTACACTGAGATATCTGATGAATGTAGAAAAGTTGATGAAGATGGCCGGTTCTGTTAGAACCGGTGGAAAGGGTACAGTCAGAAGAAAGAAGAAGGCTGTCCACAAGACAGCTACTACAGACGACAAAAGACTTCAAAGCACTCTGAAGAGAATTGGAGTGAATGGTATCCCTGCCATTGAGGAAGTCAACATATTTAAGGATGATGTAGTTATTCAGTTTCTAAACCCCAAAGTACAAGCATCTATTGCTGCCAATACTTGGGTTGTTAGCGGTTCTCCTCAGACAAAGAAGTTGCAGGATATTCTCCCCAACATCATTCACCAATTAGGGCCAGATAATTTGGAAAATCTGAAGAAGCTAGCTGAGCAATTTCAGAAGCAGGCTCCTGAAGCAGGAGCTGATGCAACTGCAGCACAAGAGGATGATGATGATGCTGTACCAGATCTTGTCCCCGGGGAGACCTTTGAAACTGCTGCTGAGGAGACCAAGGCTTGA

Protein sequence

>MsG0380012279.01.T01

MRRCRVGVGHRRVSNTGTLLIRGVSVLHRFLQCMFTLRYLMNVEKLMKMAGSVRTGGKGTVRRKKKAVHKTATTDDKRLQSTLKRIGVNGIPAIEEVNIFKDDVVIQFLNPKVQASIAANTWVVSGSPQTKKLQDILPNIIHQLGPDNLENLKKLAEQFQKQAPEAGADATAAQEDDDDAVPDLVPGETFETAAEETKA*

>MsG0380012279.01.T02

MRRCRVGVGHRRVSNTGTLLIRGVSVLHRFLQCMFTLRYLMNVEKLMKMAGSVRTGGKGTVRRKKKAVHKTATTDDKRLQSTLKRIGVNGIPAIEEVNIFKDDVVIQFLNPKVQASIAANTWVVSGSPQTKKLQDILPNIIHQLGPDNLENLKKLAEQFQKQAPEAGADATAAQEDDDDAVPDLVPGETFETAAEETKA*