AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001034.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001034.01.T01 MTR_1g029150 96.765 340 9 1 1 338 1 340 0.0 685
MsG0180001034.01.T01 MTR_3g090100 77.551 294 66 0 45 338 26 319 1.02e-174 489
MsG0180001034.01.T01 MTR_3g090020 74.026 308 78 1 33 338 12 319 1.58e-171 481
MsG0180001034.01.T01 MTR_3g089025 73.000 300 81 0 39 338 23 322 1.95e-168 473
MsG0180001034.01.T01 MTR_3g090010 72.204 313 80 1 26 338 18 323 1.03e-167 471
MsG0180001034.01.T01 MTR_4g087440 72.575 299 80 1 40 338 49 345 2.66e-166 469
MsG0180001034.01.T01 MTR_5g024130 72.575 299 80 1 40 338 49 345 2.84e-166 469
MsG0180001034.01.T01 MTR_3g090110 45.872 109 36 1 230 338 2 87 3.71e-24 95.5
MsG0180001034.01.T01 MTR_3g089980 57.303 89 25 2 250 338 7 82 3.81e-22 89.4
MsG0180001034.01.T01 MTR_3g089973 69.767 43 13 0 230 272 2 44 3.98e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001034.01.T01 AT1G43800 78.641 309 63 2 33 338 32 340 0.0 516
MsG0180001034.01.T01 AT3G02630 73.220 295 77 1 42 336 53 345 5.29e-166 468
MsG0180001034.01.T01 AT2G43710 70.569 299 86 1 40 338 57 353 1.64e-161 457
MsG0180001034.01.T01 AT2G43710 70.569 299 86 1 40 338 57 353 2.28e-161 457
MsG0180001034.01.T01 AT5G16240 70.169 295 86 1 42 336 51 343 1.23e-158 449
MsG0180001034.01.T01 AT5G16230 71.080 287 82 1 50 336 63 348 4.10e-155 441
MsG0180001034.01.T01 AT3G02610 70.383 287 84 1 50 336 73 358 2.52e-153 437
MsG0180001034.01.T01 AT3G02610 70.383 287 84 1 50 336 71 356 2.83e-153 437
MsG0180001034.01.T01 AT3G02620 64.780 318 101 4 26 336 38 351 8.89e-150 427
MsG0180001034.01.T01 AT3G02620 64.780 318 101 4 26 336 35 348 1.37e-149 427
MsG0180001034.01.T01 AT5G16230 70.696 273 79 1 64 336 1 272 9.48e-147 417
MsG0180001034.01.T01 AT3G02620 68.498 273 85 1 64 336 1 272 1.40e-141 404

Find 94 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACTTATTTGTATCTTTC+TGG 0.182339 1:-14994995 MsG0180001034.01.T01:CDS
CAATGATTACGCTGATATTT+TGG 0.196546 1:-14993615 MsG0180001034.01.T01:CDS
AAAACTGTTCAGTATCTTAT+TGG 0.203529 1:-14994947 MsG0180001034.01.T01:CDS
GTGTATACAAATCCCAAGTA+TGG 0.240289 1:+14993944 None:intergenic
TGTTGTGTTGGTTGGTGATA+TGG 0.248245 1:-14995161 MsG0180001034.01.T01:CDS
GTGGTGGTGATGGTGGATTT+TGG 0.262583 1:+14995415 None:intergenic
TTTGGAATGTTCCATGTTAA+TGG 0.266021 1:+14995478 None:intergenic
TGGTGGCATTGAGTGGGTTT+TGG 0.304471 1:+14995372 None:intergenic
GTCCAAGCCCGTGGGCCGTT+TGG 0.332806 1:-14995063 MsG0180001034.01.T01:CDS
TCTTTCTGGAAGGGTTGATA+TGG 0.335225 1:-14994981 MsG0180001034.01.T01:CDS
GTCCAAACGGCCCACGGGCT+TGG 0.340343 1:+14995061 None:intergenic
GCGGTGTGGTCGGTGGTTGA+TGG 0.380740 1:+14995453 None:intergenic
GGCTGCCAGCATTGTTCCAC+TGG 0.386228 1:+14995283 None:intergenic
GGGCCGTTTGGACACGGGCT+TGG 0.396447 1:-14995051 MsG0180001034.01.T01:CDS
AACGGCCCACGGGCTTGGAC+TGG 0.398332 1:+14995066 None:intergenic
TTTAATCGAACGGTGGAGAT+TGG 0.401193 1:-14993588 MsG0180001034.01.T01:CDS
ATACAAATGTTGCTCGCTCT+TGG 0.405320 1:+14993915 None:intergenic
GACAGAAAACAACCCATACT+TGG 0.410965 1:-14993957 MsG0180001034.01.T01:CDS
ATTTGATGAATTTACTGATC+AGG 0.419139 1:-14995230 MsG0180001034.01.T01:CDS
TCAAATGGCAATGAAGGATC+AGG 0.420107 1:+14995247 None:intergenic
GCGAAGTGCTCGAATAGCTT+AGG 0.427800 1:+14993671 None:intergenic
CAAAATATCAGCGTAATCAT+TGG 0.433099 1:+14993616 None:intergenic
GCCCGTGGGCCGTTTGGACA+CGG 0.438549 1:-14995057 MsG0180001034.01.T01:CDS
GTAATAGGTGGCGGTGTGGT+CGG 0.450987 1:+14995443 None:intergenic
TTTGCATTGGACAGGACCCC+GGG 0.454311 1:-14993979 MsG0180001034.01.T01:intron
CGACGGTGGTGTGGTGGTGA+TGG 0.457737 1:+14995405 None:intergenic
TGATCAGTAAATTCATCAAA+TGG 0.459663 1:+14995232 None:intergenic
ATAGACATGGGGATTTGCTT+AGG 0.460553 1:-14995015 MsG0180001034.01.T01:CDS
CCACATATGCGCGCAAGCAC+TGG 0.472837 1:+14993851 None:intergenic
ATTCGAGCACTTCGCGGCCG+TGG 0.474737 1:-14993663 MsG0180001034.01.T01:CDS
GTTCAGTATCTTATTGGAGC+TGG 0.477043 1:-14994941 MsG0180001034.01.T01:CDS
AATTCATCAAATGGCAATGA+AGG 0.478762 1:+14995241 None:intergenic
CGAAGTGCTCGAATAGCTTA+GGG 0.481594 1:+14993672 None:intergenic
AATGGCTAGCATTGCTCCTG+TGG 0.488560 1:+14993754 None:intergenic
AACACAACAAAATACTCATC+AGG 0.489365 1:+14995175 None:intergenic
CCGGCACACCTTATGTATGA+TGG 0.493314 1:-14993701 MsG0180001034.01.T01:CDS
TGGAAAAGCTTGAAGGATAA+CGG 0.493538 1:-14993568 None:intergenic
GGTCTTGTCCATCATACATA+AGG 0.494290 1:+14993693 None:intergenic
TTATTTGTATCTTTCTGGAA+GGG 0.495330 1:-14994990 MsG0180001034.01.T01:CDS
GCCCGTGTCCAAACGGCCCA+CGG 0.499533 1:+14995055 None:intergenic
CGGTGGTGTGGTGGTGATGG+TGG 0.503987 1:+14995408 None:intergenic
GATGGTGTTGGTGATGAGGT+TGG 0.504177 1:-14995091 MsG0180001034.01.T01:CDS
GCGGCCGTGGCACAGCGCAC+CGG 0.504431 1:-14993650 MsG0180001034.01.T01:CDS
ATGGCTAGCATTGCTCCTGT+GGG 0.506752 1:+14993755 None:intergenic
CCCGTGGGCCGTTTGGACAC+GGG 0.513039 1:-14995056 MsG0180001034.01.T01:CDS
CCAGTGCTTGCGCGCATATG+TGG 0.513295 1:-14993851 MsG0180001034.01.T01:CDS
AACTCCGGTGCGCTGTGCCA+CGG 0.520106 1:+14993646 None:intergenic
TCTTGATGGTGTTGGTGATG+AGG 0.523044 1:-14995095 MsG0180001034.01.T01:CDS
AGTCCAAGCCCGTGTCCAAA+CGG 0.523809 1:+14995048 None:intergenic
TGGAGATTGGAAAAGCTTGA+AGG 0.530283 1:-14993575 MsG0180001034.01.T01:CDS
TAAAGCCAGTGGAACAATGC+TGG 0.530542 1:-14995288 MsG0180001034.01.T01:CDS
TTTCTCCATCATATCACCAA+TGG 0.532359 1:+14993736 None:intergenic
GTATCTTATTGGAGCTGGCA+TGG 0.533582 1:-14994936 MsG0180001034.01.T01:intron
TTGGTTCCAGTCCAAGCCCG+TGG 0.534499 1:-14995072 MsG0180001034.01.T01:CDS
TTGGTGGTGGTAATAGGTGG+CGG 0.534962 1:+14995434 None:intergenic
TAAGCTATTCGAGCACTTCG+CGG 0.535770 1:-14993669 MsG0180001034.01.T01:CDS
GTTTGCATTGGACAGGACCC+CGG 0.538725 1:-14993980 MsG0180001034.01.T01:intron
AGTATGATCAATGGTCTTGA+TGG 0.539669 1:-14995109 MsG0180001034.01.T01:CDS
GCTAGCCATTGGTGATATGA+TGG 0.539965 1:-14993741 MsG0180001034.01.T01:CDS
CTTATTTGTATCTTTCTGGA+AGG 0.544191 1:-14994991 MsG0180001034.01.T01:CDS
CCATCATACATAAGGTGTGC+CGG 0.547215 1:+14993701 None:intergenic
ATAGGTGGCGGTGTGGTCGG+TGG 0.548279 1:+14995446 None:intergenic
TTTCAAATCACTAGAAAACT+GGG 0.554024 1:-14995335 MsG0180001034.01.T01:CDS
CGTCGAGAAGCTTCTAGAAG+TGG 0.554197 1:-14993780 MsG0180001034.01.T01:CDS
CCCGTGTCCAAACGGCCCAC+GGG 0.555548 1:+14995056 None:intergenic
TGTTCCACTGGCTTTAGTAG+TGG 0.556461 1:+14995295 None:intergenic
ACGGCTCGCTTAGCGAAAGA+AGG 0.562132 1:-14993881 MsG0180001034.01.T01:CDS
ACAGGAGCAATGCTAGCCAT+TGG 0.563098 1:-14993752 MsG0180001034.01.T01:CDS
TGTATACAAATCCCAAGTAT+GGG 0.587703 1:+14993945 None:intergenic
ACGTATCAGAGTATGATCAA+TGG 0.591704 1:-14995118 MsG0180001034.01.T01:CDS
ACAGCGCACCGGAGTTTACA+CGG 0.592150 1:-14993639 MsG0180001034.01.T01:CDS
ATCATTGGCCGTGTAAACTC+CGG 0.595715 1:+14993631 None:intergenic
TTAAGACACTTCCATTAACA+TGG 0.596362 1:-14995489 MsG0180001034.01.T01:CDS
ACAGAAAACAACCCATACTT+GGG 0.596760 1:-14993956 MsG0180001034.01.T01:CDS
CTTCTAGAAGTGGACCCCAC+AGG 0.596989 1:-14993770 MsG0180001034.01.T01:CDS
ATCAATGGTCTTGATGGTGT+TGG 0.597547 1:-14995103 MsG0180001034.01.T01:CDS
TGAATTTACTGATCAGGTGA+AGG 0.600944 1:-14995224 MsG0180001034.01.T01:CDS
GTTGGTTGGTGATATGGTGA+CGG 0.602166 1:-14995155 MsG0180001034.01.T01:CDS
TGGTTCCAGTCCAAGCCCGT+GGG 0.608947 1:-14995071 MsG0180001034.01.T01:CDS
CGAGCAACATTTGTATCACA+CGG 0.621285 1:-14993908 MsG0180001034.01.T01:CDS
ATTTGTATCACACGGAAACA+CGG 0.623224 1:-14993900 MsG0180001034.01.T01:CDS
TTGATCATACTCTGATACGT+CGG 0.624523 1:+14995121 None:intergenic
CAAATGGCAATGAAGGATCA+GGG 0.626451 1:+14995248 None:intergenic
CAACAAAATACTCATCAGGA+AGG 0.632422 1:+14995179 None:intergenic
GGTGGTAATAGGTGGCGGTG+TGG 0.634289 1:+14995439 None:intergenic
TGGCTAGCATTGCTCCTGTG+GGG 0.636911 1:+14993756 None:intergenic
CTGCTGAAGAAAATAGACAT+GGG 0.645814 1:-14995027 MsG0180001034.01.T01:CDS
TGCTGAAGAAAATAGACATG+GGG 0.668352 1:-14995026 MsG0180001034.01.T01:CDS
ACTGCTGAAGAAAATAGACA+TGG 0.671679 1:-14995028 MsG0180001034.01.T01:CDS
TTTACCACTACTAAAGCCAG+TGG 0.671826 1:-14995299 MsG0180001034.01.T01:CDS
GCTCGCTTAGCGAAAGAAGG+CGG 0.694123 1:-14993878 MsG0180001034.01.T01:CDS
GGTCCTTCGTGCGATCACGA+AGG 0.703598 1:+14995200 None:intergenic
GGATCAGGGAGAAAATTCTG+TGG 0.710660 1:+14995262 None:intergenic
GGTCCTTCGTGATCGCACGA+AGG 0.710721 1:-14995203 MsG0180001034.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAATCTATGAT+AGG - Chr1:14994585-14994604 MsG0180001034.01.T01:intron 10.0%
!! AAAAAAAAAAATCTATGATA+GGG - Chr1:14994586-14994605 MsG0180001034.01.T01:intron 10.0%
!! AAGAAAATAATTAGATAAAT+TGG - Chr1:14994255-14994274 MsG0180001034.01.T01:intron 10.0%
!!! AGATTTTTTTTTTTTTTTGA+GGG + Chr1:14994580-14994599 None:intergenic 10.0%
!!! TAGATTTTTTTTTTTTTTTG+AGG + Chr1:14994581-14994600 None:intergenic 10.0%
!! AAAAAAAAAAAAAAAAACCG+AGG + Chr1:14994644-14994663 None:intergenic 15.0%
!! CATATTATTAATTAAAGTGA+TGG - Chr1:14994292-14994311 MsG0180001034.01.T01:intron 15.0%
!!! TTTTTTACTTTCTAACTAAA+TGG - Chr1:14995043-14995062 MsG0180001034.01.T01:CDS 15.0%
!! ATATGATCTATACTAGTTAA+TGG + Chr1:14994533-14994552 None:intergenic 20.0%
!! TTGAAAAAATATAGAATCCT+CGG - Chr1:14994662-14994681 MsG0180001034.01.T01:intron 20.0%
!!! AATTGATTTTTCATGTGTTA+CGG - Chr1:14995005-14995024 MsG0180001034.01.T01:CDS 20.0%
!!! TGCTCTGTTTTTTTTTTTTT+TGG - Chr1:14994718-14994737 MsG0180001034.01.T01:intron 20.0%
! AAAACTGTTCAGTATCTTAT+TGG - Chr1:14994138-14994157 MsG0180001034.01.T01:intron 25.0%
! ACTACTTAGAAGAAAAGTTT+CGG + Chr1:14994925-14994944 None:intergenic 25.0%
! ATCTATCTAAATAGAATCCT+CGG - Chr1:14994624-14994643 MsG0180001034.01.T01:intron 25.0%
! ATTTGATGAATTTACTGATC+AGG - Chr1:14993855-14993874 MsG0180001034.01.T01:CDS 25.0%
! CAAAACAGAACATTATGTTT+CGG + Chr1:14994745-14994764 None:intergenic 25.0%
! TCATGTAACATAAAAGTGAT+GGG + Chr1:14994814-14994833 None:intergenic 25.0%
! TGATCAGTAAATTCATCAAA+TGG + Chr1:14993856-14993875 None:intergenic 25.0%
! TTCATGTAACATAAAAGTGA+TGG + Chr1:14994815-14994834 None:intergenic 25.0%
! TTTCAAATCACTAGAAAACT+GGG - Chr1:14993750-14993769 MsG0180001034.01.T01:CDS 25.0%
!! ACTTTTATGTTACATGAATG+AGG - Chr1:14994817-14994836 MsG0180001034.01.T01:intron 25.0%
!! GTAGACATTATTTTCAACTT+AGG + Chr1:14995075-14995094 None:intergenic 25.0%
!! TGTACATTTTATTGCAATCT+AGG - Chr1:14994226-14994245 MsG0180001034.01.T01:intron 25.0%
!! TTATTTGTATCTTTCTGGAA+GGG - Chr1:14994095-14994114 MsG0180001034.01.T01:intron 25.0%
!! TTTTCAAATCACTAGAAAAC+TGG - Chr1:14993749-14993768 MsG0180001034.01.T01:CDS 25.0%
!!! AATTGATTTTTCACATGTGT+GGG - Chr1:14994969-14994988 MsG0180001034.01.T01:CDS 25.0%
!!! TAATTGATTTTTCACATGTG+TGG - Chr1:14994968-14994987 MsG0180001034.01.T01:CDS 25.0%
!!! TTGAAAATTTCTAGCTTTTC+TGG + Chr1:14993736-14993755 None:intergenic 25.0%
!!! TTTTGGAGTTTTTAATCGAA+CGG - Chr1:14995487-14995506 MsG0180001034.01.T01:CDS 25.0%
AACACAACAAAATACTCATC+AGG + Chr1:14993913-14993932 None:intergenic 30.0%
AATTCATCAAATGGCAATGA+AGG + Chr1:14993847-14993866 None:intergenic 30.0%
AGGACTTATTTGTATCTTTC+TGG - Chr1:14994090-14994109 MsG0180001034.01.T01:intron 30.0%
ATGTAACATAAAAGTGATGG+GGG + Chr1:14994812-14994831 None:intergenic 30.0%
CAAAATATCAGCGTAATCAT+TGG + Chr1:14995472-14995491 None:intergenic 30.0%
CATGTAACATAAAAGTGATG+GGG + Chr1:14994813-14994832 None:intergenic 30.0%
CTTATTTGTATCTTTCTGGA+AGG - Chr1:14994094-14994113 MsG0180001034.01.T01:intron 30.0%
TGTATACAAATCCCAAGTAT+GGG + Chr1:14995143-14995162 None:intergenic 30.0%
TTAAGACACTTCCATTAACA+TGG - Chr1:14993596-14993615 MsG0180001034.01.T01:CDS 30.0%
! CAATGATTACGCTGATATTT+TGG - Chr1:14995470-14995489 MsG0180001034.01.T01:CDS 30.0%
! GCAACATTGTAATTTGTAAG+AGG + Chr1:14994507-14994526 None:intergenic 30.0%
! TTTGGAATGTTCCATGTTAA+TGG + Chr1:14993610-14993629 None:intergenic 30.0%
!! ACATTGCATTTTGTTTGCAT+TGG - Chr1:14995093-14995112 MsG0180001034.01.T01:CDS 30.0%
!! TGATGAGTATTTTGTTGTGT+TGG - Chr1:14993912-14993931 MsG0180001034.01.T01:CDS 30.0%
!! TTAAAGTGATGGATAGTAGT+AGG - Chr1:14994303-14994322 MsG0180001034.01.T01:intron 30.0%
!!! AAAATTTCTAGCTTTTCTGG+TGG + Chr1:14993733-14993752 None:intergenic 30.0%
ACAGAAAACAACCCATACTT+GGG - Chr1:14995129-14995148 MsG0180001034.01.T01:CDS 35.0%
ACGTATCAGAGTATGATCAA+TGG - Chr1:14993967-14993986 MsG0180001034.01.T01:CDS 35.0%
ACTGCTGAAGAAAATAGACA+TGG - Chr1:14994057-14994076 MsG0180001034.01.T01:intron 35.0%
ATTTGTATCACACGGAAACA+CGG - Chr1:14995185-14995204 MsG0180001034.01.T01:CDS 35.0%
CAACAAAATACTCATCAGGA+AGG + Chr1:14993909-14993928 None:intergenic 35.0%
CTGCTGAAGAAAATAGACAT+GGG - Chr1:14994058-14994077 MsG0180001034.01.T01:intron 35.0%
GGAGAAAAAAATCACAATGC+CGG - Chr1:14995365-14995384 MsG0180001034.01.T01:CDS 35.0%
GTGTATACAAATCCCAAGTA+TGG + Chr1:14995144-14995163 None:intergenic 35.0%
TGAATTTACTGATCAGGTGA+AGG - Chr1:14993861-14993880 MsG0180001034.01.T01:CDS 35.0%
TGCTGAAGAAAATAGACATG+GGG - Chr1:14994059-14994078 MsG0180001034.01.T01:intron 35.0%
TTGATCATACTCTGATACGT+CGG + Chr1:14993967-14993986 None:intergenic 35.0%
TTTCTCCATCATATCACCAA+TGG + Chr1:14995352-14995371 None:intergenic 35.0%
! AGTATGATCAATGGTCTTGA+TGG - Chr1:14993976-14993995 MsG0180001034.01.T01:intron 35.0%
!! GAGTATTTTGTTGTGTTGGT+TGG - Chr1:14993916-14993935 MsG0180001034.01.T01:CDS 35.0%
!!! GTTTTGGAGATTTTTAGCGA+CGG + Chr1:14993700-14993719 None:intergenic 35.0%
ATACAAATGTTGCTCGCTCT+TGG + Chr1:14995173-14995192 None:intergenic 40.0%
CAAATGGCAATGAAGGATCA+GGG + Chr1:14993840-14993859 None:intergenic 40.0%
CGAGCAACATTTGTATCACA+CGG - Chr1:14995177-14995196 MsG0180001034.01.T01:CDS 40.0%
GACAGAAAACAACCCATACT+TGG - Chr1:14995128-14995147 MsG0180001034.01.T01:CDS 40.0%
GTTCAGTATCTTATTGGAGC+TGG - Chr1:14994144-14994163 MsG0180001034.01.T01:intron 40.0%
TCAAATGGCAATGAAGGATC+AGG + Chr1:14993841-14993860 None:intergenic 40.0%
TGGAGATTGGAAAAGCTTGA+AGG - Chr1:14995510-14995529 MsG0180001034.01.T01:CDS 40.0%
TTTAATCGAACGGTGGAGAT+TGG - Chr1:14995497-14995516 MsG0180001034.01.T01:CDS 40.0%
TTTACCACTACTAAAGCCAG+TGG - Chr1:14993786-14993805 MsG0180001034.01.T01:CDS 40.0%
! ATAGACATGGGGATTTGCTT+AGG - Chr1:14994070-14994089 MsG0180001034.01.T01:intron 40.0%
! GGTCTTGTCCATCATACATA+AGG + Chr1:14995395-14995414 None:intergenic 40.0%
! GTTCTCATGACGCTTTTCAT+CGG + Chr1:14995271-14995290 None:intergenic 40.0%
! TGGAGTTTTTAATCGAACGG+TGG - Chr1:14995490-14995509 MsG0180001034.01.T01:CDS 40.0%
!! ATCAATGGTCTTGATGGTGT+TGG - Chr1:14993982-14994001 MsG0180001034.01.T01:intron 40.0%
!! GCATTTTGTTTGCATTGGAC+AGG - Chr1:14995098-14995117 MsG0180001034.01.T01:CDS 40.0%
!! GGATAGTAGTAGGAGTGTTT+AGG - Chr1:14994313-14994332 MsG0180001034.01.T01:intron 40.0%
!! TAGTAGTAGGAGTGTTTAGG+TGG - Chr1:14994316-14994335 MsG0180001034.01.T01:intron 40.0%
!! TCTTTCTGGAAGGGTTGATA+TGG - Chr1:14994104-14994123 MsG0180001034.01.T01:intron 40.0%
!! TGTTGTGTTGGTTGGTGATA+TGG - Chr1:14993924-14993943 MsG0180001034.01.T01:CDS 40.0%
!!! ATTTTGGTGGTGGTAATAGG+TGG + Chr1:14993657-14993676 None:intergenic 40.0%
!!! TGGATTTTGGTGGTGGTAAT+AGG + Chr1:14993660-14993679 None:intergenic 40.0%
ATCATTGGCCGTGTAAACTC+CGG + Chr1:14995457-14995476 None:intergenic 45.0%
CAACATATGCGATCAAACCG+AGG + Chr1:14994682-14994701 None:intergenic 45.0%
CCATCATACATAAGGTGTGC+CGG + Chr1:14995387-14995406 None:intergenic 45.0%
GGATCAGGGAGAAAATTCTG+TGG + Chr1:14993826-14993845 None:intergenic 45.0%
GTATCTTATTGGAGCTGGCA+TGG - Chr1:14994149-14994168 MsG0180001034.01.T01:intron 45.0%
TAAAGCCAGTGGAACAATGC+TGG - Chr1:14993797-14993816 MsG0180001034.01.T01:CDS 45.0%
TAAGCTATTCGAGCACTTCG+CGG - Chr1:14995416-14995435 MsG0180001034.01.T01:CDS 45.0%
! CGAAGTGCTCGAATAGCTTA+GGG + Chr1:14995416-14995435 None:intergenic 45.0%
! GCTAGCCATTGGTGATATGA+TGG - Chr1:14995344-14995363 MsG0180001034.01.T01:CDS 45.0%
!! CTTTTCTGGTGGCATTGAGT+GGG + Chr1:14993722-14993741 None:intergenic 45.0%
!! GTATGGGTTGTTTTCTGTCC+CGG + Chr1:14995127-14995146 None:intergenic 45.0%
!! GTTGGTTGGTGATATGGTGA+CGG - Chr1:14993930-14993949 MsG0180001034.01.T01:CDS 45.0%
!! TATGGGTTGTTTTCTGTCCC+GGG + Chr1:14995126-14995145 None:intergenic 45.0%
!! TCTTGATGGTGTTGGTGATG+AGG - Chr1:14993990-14994009 MsG0180001034.01.T01:intron 45.0%
!! TGTTCCACTGGCTTTAGTAG+TGG + Chr1:14993793-14993812 None:intergenic 45.0%
!!! TTGGAGATTTTTAGCGACGG+TGG + Chr1:14993697-14993716 None:intergenic 45.0%
!!! TTTAATTTCATTTAATATTT+TGG - Chr1:14994192-14994211 MsG0180001034.01.T01:intron 5.0%
AATGGCTAGCATTGCTCCTG+TGG + Chr1:14995334-14995353 None:intergenic 50.0%
ACAGGAGCAATGCTAGCCAT+TGG - Chr1:14995333-14995352 MsG0180001034.01.T01:CDS 50.0%
ATGGCTAGCATTGCTCCTGT+GGG + Chr1:14995333-14995352 None:intergenic 50.0%
CCGGCACACCTTATGTATGA+TGG - Chr1:14995384-14995403 MsG0180001034.01.T01:CDS 50.0%
! CGTCGAGAAGCTTCTAGAAG+TGG - Chr1:14995305-14995324 MsG0180001034.01.T01:CDS 50.0%
! GCGAAGTGCTCGAATAGCTT+AGG + Chr1:14995417-14995436 None:intergenic 50.0%
!! ATGGGTTGTTTTCTGTCCCG+GGG + Chr1:14995125-14995144 None:intergenic 50.0%
!! GATGGTGTTGGTGATGAGGT+TGG - Chr1:14993994-14994013 MsG0180001034.01.T01:intron 50.0%
!! GCTTTTCTGGTGGCATTGAG+TGG + Chr1:14993723-14993742 None:intergenic 50.0%
!! TGGTGGCATTGAGTGGGTTT+TGG + Chr1:14993716-14993735 None:intergenic 50.0%
!! TTGGTGGTGGTAATAGGTGG+CGG + Chr1:14993654-14993673 None:intergenic 50.0%
!!! GATTTTTAGCGACGGTGGTG+TGG + Chr1:14993692-14993711 None:intergenic 50.0%
!!! GGTCGGTGGTTGATGGTTTT+TGG + Chr1:14993628-14993647 None:intergenic 50.0%
!!! GTGATGGTGGATTTTGGTGG+TGG + Chr1:14993667-14993686 None:intergenic 50.0%
!!! GTGGTGATGGTGGATTTTGG+TGG + Chr1:14993670-14993689 None:intergenic 50.0%
!!! GTGGTGGTGATGGTGGATTT+TGG + Chr1:14993673-14993692 None:intergenic 50.0%
ACAGCGCACCGGAGTTTACA+CGG - Chr1:14995446-14995465 MsG0180001034.01.T01:CDS 55.0%
ACGGCTCGCTTAGCGAAAGA+AGG - Chr1:14995204-14995223 MsG0180001034.01.T01:CDS 55.0%
AGTCCAAGCCCGTGTCCAAA+CGG + Chr1:14994040-14994059 None:intergenic 55.0%
GCTCGCTTAGCGAAAGAAGG+CGG - Chr1:14995207-14995226 MsG0180001034.01.T01:CDS 55.0%
GTTTGCATTGGACAGGACCC+CGG - Chr1:14995105-14995124 MsG0180001034.01.T01:CDS 55.0%
TGGCTAGCATTGCTCCTGTG+GGG + Chr1:14995332-14995351 None:intergenic 55.0%
TTTGCATTGGACAGGACCCC+GGG - Chr1:14995106-14995125 MsG0180001034.01.T01:CDS 55.0%
! CTTCTAGAAGTGGACCCCAC+AGG - Chr1:14995315-14995334 MsG0180001034.01.T01:CDS 55.0%
! GTAATAGGTGGCGGTGTGGT+CGG + Chr1:14993645-14993664 None:intergenic 55.0%
! TTTTAGCGACGGTGGTGTGG+TGG + Chr1:14993689-14993708 None:intergenic 55.0%
!! ACGCTTTTCATCGGCTGCGA+TGG + Chr1:14995262-14995281 None:intergenic 55.0%
CCACATATGCGCGCAAGCAC+TGG + Chr1:14995237-14995256 None:intergenic 60.0%
CCAGTGCTTGCGCGCATATG+TGG - Chr1:14995234-14995253 MsG0180001034.01.T01:CDS 60.0%
GGCTGCCAGCATTGTTCCAC+TGG + Chr1:14993805-14993824 None:intergenic 60.0%
GGTCCTTCGTGATCGCACGA+AGG - Chr1:14993882-14993901 MsG0180001034.01.T01:CDS 60.0%
GGTCCTTCGTGCGATCACGA+AGG + Chr1:14993888-14993907 None:intergenic 60.0%
TGGTTCCAGTCCAAGCCCGT+GGG - Chr1:14994014-14994033 MsG0180001034.01.T01:intron 60.0%
TTGGTTCCAGTCCAAGCCCG+TGG - Chr1:14994013-14994032 MsG0180001034.01.T01:intron 60.0%
! GGTGGTAATAGGTGGCGGTG+TGG + Chr1:14993649-14993668 None:intergenic 60.0%
AACTCCGGTGCGCTGTGCCA+CGG + Chr1:14995442-14995461 None:intergenic 65.0%
ATTCGAGCACTTCGCGGCCG+TGG - Chr1:14995422-14995441 MsG0180001034.01.T01:CDS 65.0%
GCGGTGTGGTCGGTGGTTGA+TGG + Chr1:14993635-14993654 None:intergenic 65.0%
! ATAGGTGGCGGTGTGGTCGG+TGG + Chr1:14993642-14993661 None:intergenic 65.0%
! CGACGGTGGTGTGGTGGTGA+TGG + Chr1:14993683-14993702 None:intergenic 65.0%
!! CGGTGGTGTGGTGGTGATGG+TGG + Chr1:14993680-14993699 None:intergenic 65.0%
AACGGCCCACGGGCTTGGAC+TGG + Chr1:14994022-14994041 None:intergenic 70.0%
CCCGTGGGCCGTTTGGACAC+GGG - Chr1:14994029-14994048 MsG0180001034.01.T01:intron 70.0%
CCCGTGTCCAAACGGCCCAC+GGG + Chr1:14994032-14994051 None:intergenic 70.0%
GCCCGTGGGCCGTTTGGACA+CGG - Chr1:14994028-14994047 MsG0180001034.01.T01:intron 70.0%
GCCCGTGTCCAAACGGCCCA+CGG + Chr1:14994033-14994052 None:intergenic 70.0%
GGGCCGTTTGGACACGGGCT+TGG - Chr1:14994034-14994053 MsG0180001034.01.T01:intron 70.0%
GTCCAAACGGCCCACGGGCT+TGG + Chr1:14994027-14994046 None:intergenic 70.0%
GTCCAAGCCCGTGGGCCGTT+TGG - Chr1:14994022-14994041 MsG0180001034.01.T01:intron 70.0%
! GCGGCCGTGGCACAGCGCAC+CGG - Chr1:14995435-14995454 MsG0180001034.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 14993571 14995536 14993571 ID=MsG0180001034.01;Name=MsG0180001034.01
Chr1 mRNA 14993571 14995536 14993571 ID=MsG0180001034.01.T01;Parent=MsG0180001034.01;Name=MsG0180001034.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|2|0|338
Chr1 exon 14994937 14995536 14994937 ID=MsG0180001034.01.T01:exon:4713;Parent=MsG0180001034.01.T01
Chr1 exon 14993571 14993987 14993571 ID=MsG0180001034.01.T01:exon:4714;Parent=MsG0180001034.01.T01
Chr1 CDS 14994937 14995536 14994937 ID=MsG0180001034.01.T01:cds;Parent=MsG0180001034.01.T01
Chr1 CDS 14993571 14993987 14993571 ID=MsG0180001034.01.T01:cds;Parent=MsG0180001034.01.T01
Gene Sequence

>MsG0180001034.01.T01

ATGCATATTCAAACTTGTCACTCAATTAAGACACTTCCATTAACATGGAACATTCCAAAAACCATCAACCACCGACCACACCGCCACCTATTACCACCACCAAAATCCACCATCACCACCACACCACCGTCGCTAAAAATCTCCAAAACCCACTCAATGCCACCAGAAAAGCTAGAAATTTTCAAATCACTAGAAAACTGGGCTTCTGAGTCTGTTTTACCACTACTAAAGCCAGTGGAACAATGCTGGCAGCCACAGAATTTTCTCCCTGATCCTTCATTGCCATTTGATGAATTTACTGATCAGGTGAAGGTCCTTCGTGATCGCACGAAGGACCTTCCTGATGAGTATTTTGTTGTGTTGGTTGGTGATATGGTGACGGAAGACGCACTTCCGACGTATCAGAGTATGATCAATGGTCTTGATGGTGTTGGTGATGAGGTTGGTTCCAGTCCAAGCCCGTGGGCCGTTTGGACACGGGCTTGGACTGCTGAAGAAAATAGACATGGGGATTTGCTTAGGACTTATTTGTATCTTTCTGGAAGGGTTGATATGGAGATGATTGAGAAAACTGTTCAGTATCTTATTGGAGCTGGCATGGACCCCGGGACAGAAAACAACCCATACTTGGGATTTGTATACACATCATTCCAAGAGCGAGCAACATTTGTATCACACGGAAACACGGCTCGCTTAGCGAAAGAAGGCGGTGATCCAGTGCTTGCGCGCATATGTGGTACCATCGCAGCCGATGAAAAGCGTCATGAGAACGCATACTCAAAAATCGTCGAGAAGCTTCTAGAAGTGGACCCCACAGGAGCAATGCTAGCCATTGGTGATATGATGGAGAAAAAAATCACAATGCCGGCACACCTTATGTATGATGGACAAGACCCTAAGCTATTCGAGCACTTCGCGGCCGTGGCACAGCGCACCGGAGTTTACACGGCCAATGATTACGCTGATATTTTGGAGTTTTTAATCGAACGGTGGAGATTGGAAAAGCTTGAAGGATAA
Protein sequence

>MsG0180001034.01.T01

MHIQTCHSIKTLPLTWNIPKTINHRPHRHLLPPPKSTITTTPPSLKISKTHSMPPEKLEIFKSLENWASESVLPLLKPVEQCWQPQNFLPDPSLPFDEFTDQVKVLRDRTKDLPDEYFVVLVGDMVTEDALPTYQSMINGLDGVGDEVGSSPSPWAVWTRAWTAEENRHGDLLRTYLYLSGRVDMEMIEKTVQYLIGAGMDPGTENNPYLGFVYTSFQERATFVSHGNTARLAKEGGDPVLARICGTIAADEKRHENAYSKIVEKLLEVDPTGAMLAIGDMMEKKITMPAHLMYDGQDPKLFEHFAAVAQRTGVYTANDYADILEFLIERWRLEKLEG*