Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001243.01.T01 | KEH40705.1 | 98.496 | 133 | 2 | 0 | 1 | 133 | 3 | 135 | 1.30E-91 | 271 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001243.01.T01 | Q9FNN6 | 61.481 | 135 | 47 | 5 | 1 | 135 | 3 | 132 | 6.32E-46 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001243.01.T01 | A0A072VRE4 | 98.496 | 133 | 2 | 0 | 1 | 133 | 3 | 135 | 6.22e-92 | 271 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180001243.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000546.01 | MsG0180001243.01 | 0.803395 | 3.558253e-49 | 1.786111e-46 |
| MsG0180001243.01 | MsG0280009168.01 | 0.806978 | 6.324811e-50 | 3.480958e-47 |
| MsG0180001243.01 | MsG0280011376.01 | 0.806861 | 6.696630e-50 | 3.674546e-47 |
| MsG0180001243.01 | MsG0380016823.01 | -0.810943 | 8.964433e-51 | 5.477393e-48 |
| MsG0180001243.01 | MsG0380017126.01 | 0.827534 | 1.483855e-54 | 1.436423e-51 |
| MsG0180001243.01 | MsG0480020521.01 | 0.809426 | 1.903514e-50 | 1.117278e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001243.01.T01 | MTR_1g033600 | 98.496 | 133 | 2 | 0 | 1 | 133 | 3 | 135 | 1.58e-95 | 271 |
| MsG0180001243.01.T01 | MTR_8g063600 | 58.519 | 135 | 41 | 2 | 1 | 135 | 1 | 120 | 9.33e-45 | 148 |
| MsG0180001243.01.T01 | MTR_8g063870 | 50.370 | 135 | 38 | 4 | 1 | 135 | 1 | 106 | 1.22e-30 | 111 |
| MsG0180001243.01.T01 | MTR_4g111975 | 45.890 | 146 | 58 | 6 | 10 | 135 | 8 | 152 | 2.11e-28 | 106 |
| MsG0180001243.01.T01 | MTR_3g462790 | 38.060 | 134 | 76 | 2 | 2 | 135 | 35 | 161 | 2.94e-28 | 106 |
| MsG0180001243.01.T01 | MTR_7g067080 | 41.007 | 139 | 60 | 3 | 5 | 135 | 30 | 154 | 1.05e-26 | 102 |
| MsG0180001243.01.T01 | MTR_1g022290 | 51.807 | 83 | 40 | 0 | 4 | 86 | 1 | 83 | 3.15e-25 | 92.4 |
| MsG0180001243.01.T01 | MTR_1g111830 | 43.939 | 132 | 55 | 3 | 8 | 135 | 22 | 138 | 6.58e-25 | 95.9 |
| MsG0180001243.01.T01 | MTR_5g081860 | 43.750 | 128 | 59 | 5 | 8 | 135 | 25 | 139 | 6.94e-25 | 96.3 |
| MsG0180001243.01.T01 | MTR_5g069710 | 38.060 | 134 | 65 | 3 | 7 | 135 | 26 | 146 | 3.18e-23 | 92.4 |
| MsG0180001243.01.T01 | MTR_3g111880 | 55.072 | 69 | 31 | 0 | 7 | 75 | 4 | 72 | 4.58e-21 | 81.6 |
| MsG0180001243.01.T01 | MTR_3g116720 | 50.000 | 68 | 34 | 0 | 8 | 75 | 14 | 81 | 4.68e-20 | 79.0 |
| MsG0180001243.01.T01 | MTR_5g020170 | 48.684 | 76 | 39 | 0 | 5 | 80 | 14 | 89 | 7.74e-20 | 79.0 |
| MsG0180001243.01.T01 | MTR_7g089210 | 56.250 | 64 | 28 | 0 | 13 | 76 | 15 | 78 | 3.06e-19 | 77.0 |
| MsG0180001243.01.T01 | MTR_6g004250 | 50.685 | 73 | 36 | 0 | 1 | 73 | 1 | 73 | 3.56e-19 | 76.6 |
| MsG0180001243.01.T01 | MTR_3g111920 | 41.791 | 67 | 39 | 0 | 10 | 76 | 3 | 69 | 2.28e-16 | 69.7 |
| MsG0180001243.01.T01 | MTR_8g077360 | 39.726 | 73 | 43 | 1 | 7 | 79 | 2 | 73 | 6.86e-16 | 68.2 |
| MsG0180001243.01.T01 | MTR_8g077380 | 40.000 | 70 | 41 | 1 | 7 | 76 | 2 | 70 | 2.15e-15 | 66.6 |
| MsG0180001243.01.T01 | MTR_8g077390 | 40.000 | 70 | 41 | 1 | 7 | 76 | 2 | 70 | 2.15e-15 | 66.6 |
| MsG0180001243.01.T01 | MTR_3g013440 | 45.205 | 73 | 39 | 1 | 1 | 72 | 1 | 73 | 3.52e-15 | 66.6 |
| MsG0180001243.01.T01 | MTR_8g077420 | 42.857 | 63 | 35 | 1 | 8 | 70 | 3 | 64 | 2.10e-13 | 61.6 |
| MsG0180001243.01.T01 | MTR_5g020540 | 46.032 | 63 | 33 | 1 | 8 | 70 | 2 | 63 | 5.06e-13 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001243.01.T01 | AT5G08520 | 61.481 | 135 | 47 | 5 | 1 | 135 | 3 | 132 | 6.45e-47 | 154 |
| MsG0180001243.01.T01 | AT5G05790 | 43.077 | 130 | 61 | 4 | 7 | 135 | 28 | 145 | 9.69e-26 | 98.6 |
| MsG0180001243.01.T01 | AT5G05790 | 43.077 | 130 | 61 | 4 | 7 | 135 | 28 | 145 | 9.69e-26 | 98.6 |
| MsG0180001243.01.T01 | AT2G38090 | 39.231 | 130 | 76 | 2 | 8 | 135 | 27 | 155 | 1.84e-25 | 98.6 |
| MsG0180001243.01.T01 | AT3G11280 | 40.945 | 127 | 56 | 3 | 10 | 135 | 33 | 141 | 6.70e-25 | 96.3 |
| MsG0180001243.01.T01 | AT3G11280 | 40.945 | 127 | 56 | 3 | 10 | 135 | 33 | 141 | 6.70e-25 | 96.3 |
| MsG0180001243.01.T01 | AT5G58900 | 40.000 | 130 | 70 | 4 | 8 | 135 | 32 | 155 | 3.50e-24 | 94.7 |
| MsG0180001243.01.T01 | AT5G23650 | 41.732 | 127 | 71 | 2 | 3 | 129 | 6 | 129 | 6.76e-24 | 94.7 |
| MsG0180001243.01.T01 | AT4G39250 | 51.948 | 77 | 37 | 0 | 10 | 86 | 14 | 90 | 7.00e-23 | 86.7 |
| MsG0180001243.01.T01 | AT1G49010 | 39.310 | 145 | 69 | 4 | 8 | 135 | 6 | 148 | 8.69e-23 | 91.7 |
| MsG0180001243.01.T01 | AT1G75250 | 50.000 | 74 | 37 | 0 | 8 | 81 | 10 | 83 | 9.94e-22 | 83.6 |
| MsG0180001243.01.T01 | AT1G75250 | 50.000 | 74 | 37 | 0 | 8 | 81 | 10 | 83 | 2.09e-21 | 83.6 |
| MsG0180001243.01.T01 | AT2G21650 | 51.429 | 70 | 34 | 0 | 10 | 79 | 14 | 83 | 1.44e-18 | 75.9 |
| MsG0180001243.01.T01 | AT1G19510 | 45.946 | 74 | 40 | 0 | 13 | 86 | 15 | 88 | 1.50e-18 | 75.5 |
| MsG0180001243.01.T01 | AT5G01200 | 34.286 | 140 | 73 | 5 | 8 | 135 | 28 | 160 | 1.06e-17 | 77.4 |
| MsG0180001243.01.T01 | AT2G18328 | 50.794 | 63 | 31 | 0 | 14 | 76 | 15 | 77 | 4.30e-17 | 71.2 |
| MsG0180001243.01.T01 | AT5G04760 | 34.109 | 129 | 67 | 4 | 7 | 135 | 3 | 113 | 5.20e-16 | 72.0 |
Find 46 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAATCAAAATTGGCATAA+AGG | 0.323067 | 1:-18096530 | None:intergenic |
| AAGTTAAAAGTAATCAAAAT+TGG | 0.330013 | 1:-18096538 | None:intergenic |
| GAGCTCTTGGTTGATGATAT+TGG | 0.334244 | 1:+18096285 | MsG0180001243.01.T01:CDS |
| CGATGTACCGGGGAAGACTA+TGG | 0.416693 | 1:+18096242 | MsG0180001243.01.T01:CDS |
| CGAGTCTAACCATGGAACTA+AGG | 0.427854 | 1:+18096431 | MsG0180001243.01.T01:CDS |
| GCCATCGTATAATTCTTCTT+CGG | 0.436648 | 1:+18096335 | MsG0180001243.01.T01:CDS |
| ATGCTAGCGATGAAGGAGTT+GGG | 0.443412 | 1:+18096373 | MsG0180001243.01.T01:CDS |
| TAAGCAGCATTATGAGCTCT+TGG | 0.453346 | 1:+18096272 | MsG0180001243.01.T01:CDS |
| ACAATAGCTCTGAGTGGAGC+TGG | 0.456097 | 1:+18096142 | MsG0180001243.01.T01:CDS |
| GAAGATTGCGGCCGATGTAC+CGG | 0.462750 | 1:+18096230 | MsG0180001243.01.T01:CDS |
| ACAAGTCATGCTAGCGATGA+AGG | 0.470619 | 1:+18096366 | MsG0180001243.01.T01:CDS |
| GCACACAACCAGCCTCAATC+TGG | 0.478933 | 1:-18096308 | None:intergenic |
| ATCGTATAATTCTTCTTCGG+AGG | 0.495952 | 1:+18096338 | MsG0180001243.01.T01:CDS |
| CTGAGGATGATTCGGATCGA+TGG | 0.497129 | 1:+18096205 | MsG0180001243.01.T01:CDS |
| ATCTTGAGCCCTTAGTTCCA+TGG | 0.501596 | 1:-18096440 | None:intergenic |
| GACTATGACTATGGATGAAG+TGG | 0.502959 | 1:+18096119 | None:intergenic |
| GACGTATCCTGAGGATGATT+CGG | 0.503412 | 1:+18096197 | MsG0180001243.01.T01:CDS |
| AAGATTGCGGCCGATGTACC+GGG | 0.505203 | 1:+18096231 | MsG0180001243.01.T01:CDS |
| CATGCTAGCGATGAAGGAGT+TGG | 0.506146 | 1:+18096372 | MsG0180001243.01.T01:CDS |
| ATTTCTTCCATAGTCTTCCC+CGG | 0.518652 | 1:-18096249 | None:intergenic |
| AACGACGAAAGGGTATTGCT+TGG | 0.523028 | 1:+18096472 | MsG0180001243.01.T01:CDS |
| TAAGGCATTTGAAAATGCCT+TGG | 0.529052 | 1:+18096173 | MsG0180001243.01.T01:CDS |
| TGATGATATTGGCCAGATTG+AGG | 0.549035 | 1:+18096296 | MsG0180001243.01.T01:CDS |
| TGAGGATGATTCGGATCGAT+GGG | 0.549268 | 1:+18096206 | MsG0180001243.01.T01:CDS |
| CATCGATCCGAATCATCCTC+AGG | 0.553968 | 1:-18096204 | None:intergenic |
| GATATTGGCCAGATTGAGGC+TGG | 0.554254 | 1:+18096300 | MsG0180001243.01.T01:CDS |
| TGCTAGCGATGAAGGAGTTG+GGG | 0.556566 | 1:+18096374 | MsG0180001243.01.T01:CDS |
| GGGAAGAAGGGTGGTCACAA+TGG | 0.559678 | 1:+18096396 | MsG0180001243.01.T01:CDS |
| ATCTGATCAAGAACGACGAA+AGG | 0.561791 | 1:+18096461 | MsG0180001243.01.T01:CDS |
| CAATAGCTCTGAGTGGAGCT+GGG | 0.561880 | 1:+18096143 | MsG0180001243.01.T01:CDS |
| TCGTATAATTCTTCTTCGGA+GGG | 0.565463 | 1:+18096339 | MsG0180001243.01.T01:CDS |
| TCTGATCAAGAACGACGAAA+GGG | 0.570329 | 1:+18096462 | MsG0180001243.01.T01:CDS |
| GTGGAGCTGGGAGCAAGATA+AGG | 0.570448 | 1:+18096155 | MsG0180001243.01.T01:CDS |
| CATAGTCTTCCCCGGTACAT+CGG | 0.575851 | 1:-18096241 | None:intergenic |
| GAGTCTAACCATGGAACTAA+GGG | 0.584526 | 1:+18096432 | MsG0180001243.01.T01:CDS |
| AAAGGGTATTGCTTGGACTG+AGG | 0.609474 | 1:+18096479 | MsG0180001243.01.T01:CDS |
| GAAGAATTATACGATGGCAA+AGG | 0.633833 | 1:-18096330 | None:intergenic |
| GGATCGATGGGAGAAGATTG+CGG | 0.637780 | 1:+18096218 | MsG0180001243.01.T01:CDS |
| TGCCTTGGCGACGTATCCTG+AGG | 0.642436 | 1:+18096188 | MsG0180001243.01.T01:CDS |
| GCTAGCGATGAAGGAGTTGG+GGG | 0.644660 | 1:+18096375 | MsG0180001243.01.T01:CDS |
| ATCCTCAGGATACGTCGCCA+AGG | 0.650046 | 1:-18096190 | None:intergenic |
| AGATTGCGGCCGATGTACCG+GGG | 0.676094 | 1:+18096232 | MsG0180001243.01.T01:CDS |
| TCCGAAGAAGAATTATACGA+TGG | 0.694503 | 1:-18096336 | None:intergenic |
| AAGTGGACAATAGCTCTGAG+TGG | 0.698553 | 1:+18096136 | MsG0180001243.01.T01:CDS |
| CTTGGACTGAGGAAGAACAC+AGG | 0.712971 | 1:+18096490 | MsG0180001243.01.T01:CDS |
| CTAGCGATGAAGGAGTTGGG+GGG | 0.726058 | 1:+18096376 | MsG0180001243.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTAATCAAAATTGGCATAA+AGG | - | Chr1:18096533-18096552 | None:intergenic | 25.0% |
| ATCGTATAATTCTTCTTCGG+AGG | + | Chr1:18096338-18096357 | MsG0180001243.01.T01:CDS | 35.0% | |
| GAAGAATTATACGATGGCAA+AGG | - | Chr1:18096333-18096352 | None:intergenic | 35.0% | |
| GCCATCGTATAATTCTTCTT+CGG | + | Chr1:18096335-18096354 | MsG0180001243.01.T01:CDS | 35.0% | |
| TCCGAAGAAGAATTATACGA+TGG | - | Chr1:18096339-18096358 | None:intergenic | 35.0% | |
| TCGTATAATTCTTCTTCGGA+GGG | + | Chr1:18096339-18096358 | MsG0180001243.01.T01:CDS | 35.0% | |
| ! | TAAGGCATTTGAAAATGCCT+TGG | + | Chr1:18096173-18096192 | MsG0180001243.01.T01:CDS | 35.0% |
| AAAAACGACGAGTCTAACCA+TGG | + | Chr1:18096423-18096442 | MsG0180001243.01.T01:CDS | 40.0% | |
| ATCTGATCAAGAACGACGAA+AGG | + | Chr1:18096461-18096480 | MsG0180001243.01.T01:CDS | 40.0% | |
| ATTTCTTCCATAGTCTTCCC+CGG | - | Chr1:18096252-18096271 | None:intergenic | 40.0% | |
| GAGTCTAACCATGGAACTAA+GGG | + | Chr1:18096432-18096451 | MsG0180001243.01.T01:CDS | 40.0% | |
| TAAGCAGCATTATGAGCTCT+TGG | + | Chr1:18096272-18096291 | MsG0180001243.01.T01:CDS | 40.0% | |
| TCTGATCAAGAACGACGAAA+GGG | + | Chr1:18096462-18096481 | MsG0180001243.01.T01:CDS | 40.0% | |
| TGATGATATTGGCCAGATTG+AGG | + | Chr1:18096296-18096315 | MsG0180001243.01.T01:CDS | 40.0% | |
| !! | GAGCTCTTGGTTGATGATAT+TGG | + | Chr1:18096285-18096304 | MsG0180001243.01.T01:CDS | 40.0% |
| ACAAGTCATGCTAGCGATGA+AGG | + | Chr1:18096366-18096385 | MsG0180001243.01.T01:CDS | 45.0% | |
| ATCTTGAGCCCTTAGTTCCA+TGG | - | Chr1:18096443-18096462 | None:intergenic | 45.0% | |
| ATGCTAGCGATGAAGGAGTT+GGG | + | Chr1:18096373-18096392 | MsG0180001243.01.T01:CDS | 45.0% | |
| CGAGTCTAACCATGGAACTA+AGG | + | Chr1:18096431-18096450 | MsG0180001243.01.T01:CDS | 45.0% | |
| GACGTATCCTGAGGATGATT+CGG | + | Chr1:18096197-18096216 | MsG0180001243.01.T01:CDS | 45.0% | |
| ! | TGAGGATGATTCGGATCGAT+GGG | + | Chr1:18096206-18096225 | MsG0180001243.01.T01:CDS | 45.0% |
| !! | AAAGGGTATTGCTTGGACTG+AGG | + | Chr1:18096479-18096498 | MsG0180001243.01.T01:CDS | 45.0% |
| !! | AACGACGAAAGGGTATTGCT+TGG | + | Chr1:18096472-18096491 | MsG0180001243.01.T01:CDS | 45.0% |
| !! | AAGTGGACAATAGCTCTGAG+TGG | + | Chr1:18096136-18096155 | MsG0180001243.01.T01:CDS | 45.0% |
| !!! | GGTTAGACTCGTCGTTTTTG+TGG | - | Chr1:18096422-18096441 | None:intergenic | 45.0% |
| CATAGTCTTCCCCGGTACAT+CGG | - | Chr1:18096244-18096263 | None:intergenic | 50.0% | |
| CATCGATCCGAATCATCCTC+AGG | - | Chr1:18096207-18096226 | None:intergenic | 50.0% | |
| CATGCTAGCGATGAAGGAGT+TGG | + | Chr1:18096372-18096391 | MsG0180001243.01.T01:CDS | 50.0% | |
| CTTGGACTGAGGAAGAACAC+AGG | + | Chr1:18096490-18096509 | MsG0180001243.01.T01:CDS | 50.0% | |
| GATATTGGCCAGATTGAGGC+TGG | + | Chr1:18096300-18096319 | MsG0180001243.01.T01:CDS | 50.0% | |
| GGATCGATGGGAGAAGATTG+CGG | + | Chr1:18096218-18096237 | MsG0180001243.01.T01:CDS | 50.0% | |
| TGCTAGCGATGAAGGAGTTG+GGG | + | Chr1:18096374-18096393 | MsG0180001243.01.T01:CDS | 50.0% | |
| ! | CTGAGGATGATTCGGATCGA+TGG | + | Chr1:18096205-18096224 | MsG0180001243.01.T01:CDS | 50.0% |
| !! | ACAATAGCTCTGAGTGGAGC+TGG | + | Chr1:18096142-18096161 | MsG0180001243.01.T01:CDS | 50.0% |
| !! | CAATAGCTCTGAGTGGAGCT+GGG | + | Chr1:18096143-18096162 | MsG0180001243.01.T01:CDS | 50.0% |
| AAGATTGCGGCCGATGTACC+GGG | + | Chr1:18096231-18096250 | MsG0180001243.01.T01:CDS | 55.0% | |
| ATCCTCAGGATACGTCGCCA+AGG | - | Chr1:18096193-18096212 | None:intergenic | 55.0% | |
| CGATGTACCGGGGAAGACTA+TGG | + | Chr1:18096242-18096261 | MsG0180001243.01.T01:CDS | 55.0% | |
| CTAGCGATGAAGGAGTTGGG+GGG | + | Chr1:18096376-18096395 | MsG0180001243.01.T01:CDS | 55.0% | |
| GAAGATTGCGGCCGATGTAC+CGG | + | Chr1:18096230-18096249 | MsG0180001243.01.T01:CDS | 55.0% | |
| GAAGGAGTTGGGGGGAAGAA+GGG | + | Chr1:18096384-18096403 | MsG0180001243.01.T01:CDS | 55.0% | |
| GCACACAACCAGCCTCAATC+TGG | - | Chr1:18096311-18096330 | None:intergenic | 55.0% | |
| GCTAGCGATGAAGGAGTTGG+GGG | + | Chr1:18096375-18096394 | MsG0180001243.01.T01:CDS | 55.0% | |
| GGGAAGAAGGGTGGTCACAA+TGG | + | Chr1:18096396-18096415 | MsG0180001243.01.T01:CDS | 55.0% | |
| GTGGAGCTGGGAGCAAGATA+AGG | + | Chr1:18096155-18096174 | MsG0180001243.01.T01:CDS | 55.0% | |
| TGAAGGAGTTGGGGGGAAGA+AGG | + | Chr1:18096383-18096402 | MsG0180001243.01.T01:CDS | 55.0% | |
| AGATTGCGGCCGATGTACCG+GGG | + | Chr1:18096232-18096251 | MsG0180001243.01.T01:CDS | 60.0% | |
| TGCCTTGGCGACGTATCCTG+AGG | + | Chr1:18096188-18096207 | MsG0180001243.01.T01:CDS | 60.0% | |
| GGAGTTGGGGGGAAGAAGGG+TGG | + | Chr1:18096387-18096406 | MsG0180001243.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 18096129 | 18096557 | 18096129 | ID=MsG0180001243.01;Name=MsG0180001243.01 |
| Chr1 | mRNA | 18096129 | 18096557 | 18096129 | ID=MsG0180001243.01.T01;Parent=MsG0180001243.01;Name=MsG0180001243.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|142 |
| Chr1 | exon | 18096129 | 18096557 | 18096129 | ID=MsG0180001243.01.T01:exon:32372;Parent=MsG0180001243.01.T01 |
| Chr1 | CDS | 18096129 | 18096557 | 18096129 | ID=MsG0180001243.01.T01:cds;Parent=MsG0180001243.01.T01 |
| Gene Sequence |
| Protein sequence |
