AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020521.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020521.01.T01 MTR_4g052810 87.975 158 7 2 38 183 32 189 5.15e-99 284
MsG0480020521.01.T01 MTR_4g052800 77.358 159 24 2 38 184 53 211 2.04e-87 256
MsG0480020521.01.T01 MTR_8g021060 50.311 161 45 4 38 184 20 159 3.06e-45 147
MsG0480020521.01.T01 MTR_8g021030 48.182 110 35 1 38 147 8 95 3.49e-30 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020521.01.T01 AT3G03420 60.377 159 51 2 38 184 36 194 7.11e-67 203
MsG0480020521.01.T01 AT3G03420 55.172 87 32 1 39 118 37 123 1.56e-29 107

Find 54 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCCTTTGTCGCTCCAAC+TGG 0.294022 4:-42821474 MsG0480020521.01.T01:CDS
GAGATTCTTAAGAGAACATT+TGG 0.369159 4:-42820430 MsG0480020521.01.T01:CDS
CATGCTGGCGGTTATGTTCC+CGG 0.371233 4:-42820348 MsG0480020521.01.T01:CDS
GGCGGTTATGTTCCCGGTGA+AGG 0.373640 4:-42820342 MsG0480020521.01.T01:CDS
TTGAAGAAATTATCTTGAAC+AGG 0.376229 4:+42820393 None:intergenic
AGGATTGTTCTGTGTGGTGG+TGG 0.391610 4:-42821527 MsG0480020521.01.T01:CDS
AGGAAGGCGATTGAAGGAAG+GGG 0.409881 4:-42821552 MsG0480020521.01.T01:CDS
AGAGAACATTTGGAGAAAGC+TGG 0.412333 4:-42820420 MsG0480020521.01.T01:CDS
GAGAGGAGGAAGGCGATTGA+AGG 0.424080 4:-42821558 MsG0480020521.01.T01:CDS
GAGGAAGGCGATTGAAGGAA+GGG 0.424735 4:-42821553 MsG0480020521.01.T01:CDS
TGACTGCCGAGCTGCAAACT+TGG 0.431388 4:-42819342 MsG0480020521.01.T01:CDS
GGAGGAAGGCGATTGAAGGA+AGG 0.431697 4:-42821554 MsG0480020521.01.T01:CDS
AGAATGCTATGGAAGCTGTA+TGG 0.436447 4:-42817847 MsG0480020521.01.T01:CDS
GCGGTTATGTTCCCGGTGAA+GGG 0.449770 4:-42820341 MsG0480020521.01.T01:CDS
GCCGAGCTGCAAACTTGGAT+TGG 0.462029 4:-42819337 MsG0480020521.01.T01:CDS
ATCCAAACGTACCCCTTCAC+CGG 0.464531 4:+42820329 None:intergenic
GTGGTGATTGTCATTACAAA+CGG 0.465275 4:-42819165 MsG0480020521.01.T01:CDS
TGTGTGGTGGTGGATGATGC+AGG 0.468609 4:-42821517 MsG0480020521.01.T01:CDS
AAATGCAAGACCAGGTTGAC+CGG 0.481498 4:-42819397 MsG0480020521.01.T01:CDS
GGAAGGATTGTTCTGTGTGG+TGG 0.482197 4:-42821530 MsG0480020521.01.T01:CDS
CAGGGTATCCGTGGTGGTGT+TGG 0.501821 4:-42821498 MsG0480020521.01.T01:CDS
GAATGCTATGGAAGCTGTAT+GGG 0.505706 4:-42817846 MsG0480020521.01.T01:CDS
GAGAACATTTGGAGAAAGCT+GGG 0.518773 4:-42820419 MsG0480020521.01.T01:CDS
TGGTGATTGTCATTACAAAC+GGG 0.520121 4:-42819164 MsG0480020521.01.T01:CDS
GCAGTTGAACCAGAACAAAA+CGG 0.526675 4:+42817872 None:intergenic
TTCAACTGCAAAGAATGCTA+TGG 0.543866 4:-42817858 MsG0480020521.01.T01:CDS
TCCAATCCAAGTTTGCAGCT+CGG 0.557734 4:+42819336 None:intergenic
TATCCTTTGTCGCTCCAACT+GGG 0.559657 4:-42821473 MsG0480020521.01.T01:CDS
GGAAGGCGATTGAAGGAAGG+GGG 0.561259 4:-42821551 MsG0480020521.01.T01:CDS
TATATGAAACAGATAAGAGC+TGG 0.568061 4:-42819196 MsG0480020521.01.T01:intron
GCACTATCACCCGGTCAACC+TGG 0.568691 4:+42819387 None:intergenic
ACTCCCAGTTGGAGCGACAA+AGG 0.581523 4:+42821470 None:intergenic
AATGCAAGACCAGGTTGACC+GGG 0.583326 4:-42819396 MsG0480020521.01.T01:CDS
AACAAACCTCACTACAAGCA+TGG 0.583377 4:+42819300 None:intergenic
TCTCTTAAGAATCTCGCCGT+TGG 0.587003 4:+42820438 None:intergenic
CGGTTATGTTCCCGGTGAAG+GGG 0.591105 4:-42820340 MsG0480020521.01.T01:intron
TGCTCACCATGCTTGTAGTG+AGG 0.595210 4:-42819306 MsG0480020521.01.T01:intron
GCTCGGTATGGAGAGGAGGA+AGG 0.597165 4:-42821568 None:intergenic
TCCAAACGTACCCCTTCACC+GGG 0.603119 4:+42820330 None:intergenic
CGAGGACATTTGAAAATACG+AGG 0.614137 4:-42819133 MsG0480020521.01.T01:CDS
AATTGCGATCAAAATCATGC+TGG 0.615386 4:-42820363 MsG0480020521.01.T01:CDS
ATAAGAGCTGGTCATCTAAG+TGG 0.623759 4:-42819184 MsG0480020521.01.T01:CDS
GAAAGCTCGGTATGGAGAGG+AGG 0.624719 4:-42821572 None:intergenic
AAGTGATTCCTACTCCCAGT+TGG 0.627630 4:+42821459 None:intergenic
TCAGATAGAAATGCAAGACC+AGG 0.632881 4:-42819405 MsG0480020521.01.T01:intron
GTGTGGTGGTGGATGATGCA+GGG 0.640734 4:-42821516 MsG0480020521.01.T01:CDS
TGCGATCAAAATCATGCTGG+CGG 0.640797 4:-42820360 MsG0480020521.01.T01:CDS
TGGATGATGCAGGGTATCCG+TGG 0.643058 4:-42821507 MsG0480020521.01.T01:CDS
TTAGCTCGTGCACTATCACC+CGG 0.650268 4:+42819378 None:intergenic
CTTGAAAGCTCGGTATGGAG+AGG 0.658728 4:-42821575 None:intergenic
AGGATAAACCAACACCACCA+CGG 0.679980 4:+42821490 None:intergenic
ATGATGCAGGGTATCCGTGG+TGG 0.707122 4:-42821504 MsG0480020521.01.T01:CDS
GAGGACATTTGAAAATACGA+GGG 0.729192 4:-42819132 MsG0480020521.01.T01:CDS
TACAAACGGGAACTTCTACG+AGG 0.776697 4:-42819151 MsG0480020521.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTAATATAAATTTTATCT+TGG - Chr4:42818303-42818322 MsG0480020521.01.T01:intron 10.0%
!!! TTTTCTGTTTTAAAATTTTA+TGG - Chr4:42821378-42821397 MsG0480020521.01.T01:intron 10.0%
!! ATCAATAAAAAAATGATTGA+AGG + Chr4:42820144-42820163 None:intergenic 15.0%
!! TTAAATTATTATCTTTCCAA+TGG - Chr4:42818079-42818098 MsG0480020521.01.T01:intron 15.0%
!! TTTATACATAACATTTACTT+TGG - Chr4:42820288-42820307 MsG0480020521.01.T01:intron 15.0%
!!! CAAAAACTAAAATGAAATTT+TGG + Chr4:42818537-42818556 None:intergenic 15.0%
!!! GTTTTTACAAAAATATCATA+TGG - Chr4:42818470-42818489 MsG0480020521.01.T01:intron 15.0%
!! AAAATTAAAACCCTCTTATA+TGG - Chr4:42820763-42820782 MsG0480020521.01.T01:intron 20.0%
!! AAATTATAACCATTGTAGAA+GGG + Chr4:42821280-42821299 None:intergenic 20.0%
!! CAAAATCTGTATGAATAAAA+GGG + Chr4:42820099-42820118 None:intergenic 20.0%
!! TAGTAAATTGAAAACCTTAT+AGG + Chr4:42819891-42819910 None:intergenic 20.0%
!! TATAAACAAGCTTAATTAAG+AGG - Chr4:42819251-42819270 MsG0480020521.01.T01:intron 20.0%
!! TCAAAATCTGTATGAATAAA+AGG + Chr4:42820100-42820119 None:intergenic 20.0%
!! TTAATATACATTAGAACTAG+AGG + Chr4:42818354-42818373 None:intergenic 20.0%
!!! CTAGAATTTGTATTCTTTTT+TGG - Chr4:42818240-42818259 MsG0480020521.01.T01:intron 20.0%
!!! TAAAAATCATCTAAGATGAT+TGG - Chr4:42821046-42821065 MsG0480020521.01.T01:intron 20.0%
!!! TGAAATTCTTCTTTTATCAA+TGG + Chr4:42820474-42820493 None:intergenic 20.0%
! AAGATAATTATAGGGTTAGA+TGG - Chr4:42820507-42820526 MsG0480020521.01.T01:intron 25.0%
! ACACATTATTTCAGATAATC+TGG - Chr4:42817968-42817987 MsG0480020521.01.T01:intron 25.0%
! ACGAAGAGAAGATAATTATA+GGG - Chr4:42820499-42820518 MsG0480020521.01.T01:intron 25.0%
! ATCTGTAAAGTCAGAAATAT+AGG + Chr4:42821116-42821135 None:intergenic 25.0%
! ATGAATCTGGAAATAATCTA+TGG + Chr4:42821341-42821360 None:intergenic 25.0%
! ATTATTTCAGATAATCTGGT+TGG - Chr4:42817972-42817991 MsG0480020521.01.T01:intron 25.0%
! CAAATTATAACCATTGTAGA+AGG + Chr4:42821281-42821300 None:intergenic 25.0%
! GAGAAGAAAAAAAATAGAGA+GGG + Chr4:42820909-42820928 None:intergenic 25.0%
! TCATATGATTGTTTCCTATA+AGG - Chr4:42819874-42819893 MsG0480020521.01.T01:intron 25.0%
! TCCTTAAAATTTAGCATAGA+AGG - Chr4:42818056-42818075 MsG0480020521.01.T01:intron 25.0%
! TGAATCTGGAAATAATCTAT+GGG + Chr4:42821340-42821359 None:intergenic 25.0%
! TTGAAGAAATTATCTTGAAC+AGG + Chr4:42818973-42818992 None:intergenic 25.0%
! TTTCTGACTTTAGTCTATTT+AGG - Chr4:42819627-42819646 MsG0480020521.01.T01:intron 25.0%
!! AAGACAAATATGCTTAGTTT+TGG - Chr4:42818105-42818124 MsG0480020521.01.T01:intron 25.0%
!! ACCTTCTATGCTAAATTTTA+AGG + Chr4:42818060-42818079 None:intergenic 25.0%
!! TACTTCTCAATTTTCTAACT+TGG + Chr4:42819732-42819751 None:intergenic 25.0%
!!! AGTTTTGAAACAGATACAAA+TGG + Chr4:42818660-42818679 None:intergenic 25.0%
!!! GCTTTATTTTGAAGTTAAGA+GGG - Chr4:42820649-42820668 MsG0480020521.01.T01:intron 25.0%
!!! GTTTTGAAACAGATACAAAT+GGG + Chr4:42818659-42818678 None:intergenic 25.0%
!!! TTTATTTTCGTAAGTTCTCT+AGG - Chr4:42819156-42819175 MsG0480020521.01.T01:CDS 25.0%
!!! TTTTGAAACAGATACAAATG+GGG + Chr4:42818658-42818677 None:intergenic 25.0%
AAAAGCTTAACAATAGAGAG+GGG + Chr4:42818859-42818878 None:intergenic 30.0%
ACGTATAAAAGGTAGTCAAA+AGG + Chr4:42821201-42821220 None:intergenic 30.0%
AGAAAAGCTTAACAATAGAG+AGG + Chr4:42818861-42818880 None:intergenic 30.0%
AGAGGGAAAAAGAATGTTTA+TGG + Chr4:42820892-42820911 None:intergenic 30.0%
ATTAAAACCCTCTTATATGG+AGG - Chr4:42820766-42820785 MsG0480020521.01.T01:intron 30.0%
ATTATAGGGTTAGATGGATA+AGG - Chr4:42820513-42820532 MsG0480020521.01.T01:intron 30.0%
CGTATAAAAGGTAGTCAAAA+GGG + Chr4:42821200-42821219 None:intergenic 30.0%
GAAAAGCTTAACAATAGAGA+GGG + Chr4:42818860-42818879 None:intergenic 30.0%
GACGAAGAGAAGATAATTAT+AGG - Chr4:42820498-42820517 MsG0480020521.01.T01:intron 30.0%
GAGATTCTTAAGAGAACATT+TGG - Chr4:42818933-42818952 MsG0480020521.01.T01:intron 30.0%
GCAGAATGTGCAAATTATTT+AGG - Chr4:42820627-42820646 MsG0480020521.01.T01:intron 30.0%
GCATATTTGTCTTTAACCAT+TGG + Chr4:42818098-42818117 None:intergenic 30.0%
GGAGAAGAAAAAAAATAGAG+AGG + Chr4:42820910-42820929 None:intergenic 30.0%
GTTAATTTATTCCACCTTGA+AGG - Chr4:42821428-42821447 MsG0480020521.01.T01:intron 30.0%
TATATGAAACAGATAAGAGC+TGG - Chr4:42820167-42820186 MsG0480020521.01.T01:intron 30.0%
TCTGGATTATTTGAAAGGAA+AGG - Chr4:42818001-42818020 MsG0480020521.01.T01:intron 30.0%
TTATAGGGTTAGATGGATAA+GGG - Chr4:42820514-42820533 MsG0480020521.01.T01:intron 30.0%
TTGATGTGAAACAATGAATC+TGG + Chr4:42821354-42821373 None:intergenic 30.0%
TTGTGTCTGGATTATTTGAA+AGG - Chr4:42817996-42818015 MsG0480020521.01.T01:intron 30.0%
! AAGATATTTTGTTCATGCCA+CGG - Chr4:42821079-42821098 MsG0480020521.01.T01:intron 30.0%
! TAACTCAGTATTGAACTAGT+TGG + Chr4:42821412-42821431 None:intergenic 30.0%
! TATCTGAAATAATGTGTCAG+AGG + Chr4:42817965-42817984 None:intergenic 30.0%
! TCTTCTTTTATCAATGGTAC+AGG + Chr4:42820468-42820487 None:intergenic 30.0%
! TTTGACTACCTTTTATACGT+TGG - Chr4:42821201-42821220 MsG0480020521.01.T01:intron 30.0%
!! AAATAACAAGCACTGAAGTA+AGG + Chr4:42818208-42818227 None:intergenic 30.0%
!! TGTTTTTATATCAGCTCCAA+CGG - Chr4:42818909-42818928 MsG0480020521.01.T01:intron 30.0%
!!! ATTTTGAAGTTAAGAGGGAA+AGG - Chr4:42820654-42820673 MsG0480020521.01.T01:intron 30.0%
!!! GGCTTTATTTTGAAGTTAAG+AGG - Chr4:42820648-42820667 MsG0480020521.01.T01:intron 30.0%
!!! TTGGTTTTAGTTATCATGAC+TGG - Chr4:42818124-42818143 MsG0480020521.01.T01:intron 30.0%
!!! TTTTTTTCTTCTCCCAAACA+AGG - Chr4:42820916-42820935 MsG0480020521.01.T01:intron 30.0%
AAAAAACCCCGTGTAAAGTT+GGG + Chr4:42820737-42820756 None:intergenic 35.0%
AAACTCATCCATCTGATTCA+GGG + Chr4:42818728-42818747 None:intergenic 35.0%
AACGCAGTGTTGTCAAATAT+TGG - Chr4:42819309-42819328 MsG0480020521.01.T01:CDS 35.0%
AAGGAAGGAAAAAATGGAAG+TGG - Chr4:42820697-42820716 MsG0480020521.01.T01:intron 35.0%
AATAATGTGTCAGAGGAACT+TGG + Chr4:42817958-42817977 None:intergenic 35.0%
AATTGCGATCAAAATCATGC+TGG - Chr4:42819000-42819019 MsG0480020521.01.T01:intron 35.0%
ACAGGAGTAAAAAAGTTGCT+TGG + Chr4:42820450-42820469 None:intergenic 35.0%
ACCACCATAATCTGATATGA+TGG - Chr4:42819459-42819478 MsG0480020521.01.T01:intron 35.0%
ACGCAGTGTTGTCAAATATT+GGG - Chr4:42819310-42819329 MsG0480020521.01.T01:CDS 35.0%
AGACTAAAGTCAGAAATCCT+TGG + Chr4:42819623-42819642 None:intergenic 35.0%
AGGAATCACTTTCTTCAGTT+CGG - Chr4:42817916-42817935 MsG0480020521.01.T01:intron 35.0%
ATTCTCTCAAACAGTTACCA+CGG + Chr4:42819098-42819117 None:intergenic 35.0%
CAACCATCTGATGAAATACA+CGG + Chr4:42818032-42818051 None:intergenic 35.0%
CTAATAAGTACACCATCGTA+AGG + Chr4:42819791-42819810 None:intergenic 35.0%
GAGGACATTTGAAAATACGA+GGG - Chr4:42820231-42820250 MsG0480020521.01.T01:intron 35.0%
GCCTTTCGTGCTAAAAAAAT+CGG - Chr4:42818494-42818513 MsG0480020521.01.T01:intron 35.0%
GTCTATCATTCTCAATGAAG+TGG - Chr4:42821310-42821329 MsG0480020521.01.T01:intron 35.0%
GTGGTGATTGTCATTACAAA+CGG - Chr4:42820198-42820217 MsG0480020521.01.T01:intron 35.0%
TACGCCATCATATCAGATTA+TGG + Chr4:42819466-42819485 None:intergenic 35.0%
TGACTTCTGATTCTTCAGAA+GGG + Chr4:42820994-42821013 None:intergenic 35.0%
TGGTGATTGTCATTACAAAC+GGG - Chr4:42820199-42820218 MsG0480020521.01.T01:intron 35.0%
TTATTCAACCTTGCTACTAC+TGG - Chr4:42818593-42818612 MsG0480020521.01.T01:intron 35.0%
TTCAACTGCAAAGAATGCTA+TGG - Chr4:42821505-42821524 MsG0480020521.01.T01:CDS 35.0%
TTGAGTTCCTCCATATAAGA+GGG + Chr4:42820776-42820795 None:intergenic 35.0%
! AAGTAGACAATCACTCCTTT+TGG - Chr4:42819747-42819766 MsG0480020521.01.T01:intron 35.0%
! ACCTTTTATACGTTGGACTT+GGG - Chr4:42821208-42821227 MsG0480020521.01.T01:intron 35.0%
! ATGTGAAAGCATGATTCAGA+AGG - Chr4:42818797-42818816 MsG0480020521.01.T01:intron 35.0%
! TACCTTTTATACGTTGGACT+TGG - Chr4:42821207-42821226 MsG0480020521.01.T01:intron 35.0%
! TGTGAAAGCATGATTCAGAA+GGG - Chr4:42818798-42818817 MsG0480020521.01.T01:intron 35.0%
! TTTTTACTCCAGTAGTAGCA+AGG + Chr4:42818604-42818623 None:intergenic 35.0%
!! AAAGGAGGGTTTTAACATCA+TGG - Chr4:42821460-42821479 MsG0480020521.01.T01:intron 35.0%
!! GTTGTTGTTGTCTTGTTTAG+TGG - Chr4:42819828-42819847 MsG0480020521.01.T01:intron 35.0%
!!! AGATATTGGTGGCTGTTTTT+GGG - Chr4:42819339-42819358 MsG0480020521.01.T01:CDS 35.0%
AAAAACCCCGTGTAAAGTTG+GGG + Chr4:42820736-42820755 None:intergenic 40.0%
AAAGGAATGAGAGGATTTGG+GGG - Chr4:42820672-42820691 MsG0480020521.01.T01:intron 40.0%
AACAAACCTCACTACAAGCA+TGG + Chr4:42820066-42820085 None:intergenic 40.0%
AACCATTGTAGAAGGGCAAA+AGG + Chr4:42821273-42821292 None:intergenic 40.0%
AACTCACATGCTTGAGCATT+GGG + Chr4:42821019-42821038 None:intergenic 40.0%
AAGTGTAACCCTGAATCAGA+TGG - Chr4:42818717-42818736 MsG0480020521.01.T01:intron 40.0%
ACCACCATAATCTGATACGA+TGG - Chr4:42819522-42819541 MsG0480020521.01.T01:intron 40.0%
ACTTCTGATTCTTCAGAAGG+GGG + Chr4:42820992-42821011 None:intergenic 40.0%
AGAATGCTATGGAAGCTGTA+TGG - Chr4:42821516-42821535 MsG0480020521.01.T01:CDS 40.0%
AGACATACAGAGTGTGTGAT+AGG + Chr4:42820542-42820561 None:intergenic 40.0%
AGAGAACATTTGGAGAAAGC+TGG - Chr4:42818943-42818962 MsG0480020521.01.T01:intron 40.0%
AGAGGTTATCGTTACAAACG+AGG - Chr4:42819269-42819288 MsG0480020521.01.T01:intron 40.0%
ATAAGAGCTGGTCATCTAAG+TGG - Chr4:42820179-42820198 MsG0480020521.01.T01:intron 40.0%
ATATTGGGCATGGCAGATAT+TGG - Chr4:42819325-42819344 MsG0480020521.01.T01:CDS 40.0%
CAAACTCATCCATCTGATTC+AGG + Chr4:42818729-42818748 None:intergenic 40.0%
CCTTGAAGGAATGCATCAAA+AGG - Chr4:42821442-42821461 MsG0480020521.01.T01:intron 40.0%
CCTTTGTCTTGTATGAGTGT+CGG - Chr4:42818156-42818175 MsG0480020521.01.T01:intron 40.0%
CGACACTCATACAAGACAAA+GGG + Chr4:42818158-42818177 None:intergenic 40.0%
CGAGGACATTTGAAAATACG+AGG - Chr4:42820230-42820249 MsG0480020521.01.T01:intron 40.0%
CTGACGTAAATACAATACCG+TGG - Chr4:42819078-42819097 MsG0480020521.01.T01:intron 40.0%
CTGACTTCTGATTCTTCAGA+AGG + Chr4:42820995-42821014 None:intergenic 40.0%
CTTGAGTTCCTCCATATAAG+AGG + Chr4:42820777-42820796 None:intergenic 40.0%
CTTTGTCTTGTATGAGTGTC+GGG - Chr4:42818157-42818176 MsG0480020521.01.T01:intron 40.0%
GAAAAAACCCCGTGTAAAGT+TGG + Chr4:42820738-42820757 None:intergenic 40.0%
GAAAGGAATGAGAGGATTTG+GGG - Chr4:42820671-42820690 MsG0480020521.01.T01:intron 40.0%
GAACCGTGTATTTCATCAGA+TGG - Chr4:42818026-42818045 MsG0480020521.01.T01:intron 40.0%
GAAGGAATGCATCAAAAGGA+GGG - Chr4:42821446-42821465 MsG0480020521.01.T01:intron 40.0%
GAATGCTATGGAAGCTGTAT+GGG - Chr4:42821517-42821536 MsG0480020521.01.T01:CDS 40.0%
GACTTCTGATTCTTCAGAAG+GGG + Chr4:42820993-42821012 None:intergenic 40.0%
GAGAACATTTGGAGAAAGCT+GGG - Chr4:42818944-42818963 MsG0480020521.01.T01:intron 40.0%
GCAGTTGAACCAGAACAAAA+CGG + Chr4:42821494-42821513 None:intergenic 40.0%
GCCATCATATCAGATTATGG+TGG + Chr4:42819463-42819482 None:intergenic 40.0%
GGAAAGGAATGAGAGGATTT+GGG - Chr4:42820670-42820689 MsG0480020521.01.T01:intron 40.0%
GTGTTGTCAAATATTGGGCA+TGG - Chr4:42819315-42819334 MsG0480020521.01.T01:CDS 40.0%
GTTATCGTTACAAACGAGGA+GGG - Chr4:42819273-42819292 MsG0480020521.01.T01:intron 40.0%
TATCAGATTATGGTGGTCCA+TGG + Chr4:42819456-42819475 None:intergenic 40.0%
TATTGTGCGAGATGAATCAC+TGG - Chr4:42818280-42818299 MsG0480020521.01.T01:intron 40.0%
TCAGATAGAAATGCAAGACC+AGG - Chr4:42819958-42819977 MsG0480020521.01.T01:intron 40.0%
TGAAGGAATGCATCAAAAGG+AGG - Chr4:42821445-42821464 MsG0480020521.01.T01:intron 40.0%
TGAGAGCTTTCTTCTCGATT+TGG + Chr4:42818773-42818792 None:intergenic 40.0%
TGATAGTCCCCAACTTTACA+CGG - Chr4:42820726-42820745 MsG0480020521.01.T01:intron 40.0%
TTAAGAGGGAAAGGAATGAG+AGG - Chr4:42820663-42820682 MsG0480020521.01.T01:intron 40.0%
TTTGATGCATTCCTTCAAGG+TGG + Chr4:42821442-42821461 None:intergenic 40.0%
! CCTTTTGATGCATTCCTTCA+AGG + Chr4:42821445-42821464 None:intergenic 40.0%
!! ACTCAAGAAGAGTATTCGAG+AGG - Chr4:42820790-42820809 MsG0480020521.01.T01:intron 40.0%
!! CAGATATTGGTGGCTGTTTT+TGG - Chr4:42819338-42819357 MsG0480020521.01.T01:CDS 40.0%
!!! ATTCGAGAGGTGGTTTTAGA+CGG - Chr4:42820803-42820822 MsG0480020521.01.T01:intron 40.0%
!!! GCCGATTTTTTTAGCACGAA+AGG + Chr4:42818498-42818517 None:intergenic 40.0%
AAGGAATGAGAGGATTTGGG+GGG - Chr4:42820673-42820692 MsG0480020521.01.T01:intron 45.0%
AAGTGATTCCTACTCCCAGT+TGG + Chr4:42817907-42817926 None:intergenic 45.0%
ACCTTGTGGCACTGTCATAT+CGG + Chr4:42819404-42819423 None:intergenic 45.0%
ACTGTCATATCGGACTATGG+CGG + Chr4:42819394-42819413 None:intergenic 45.0%
AGGATAAACCAACACCACCA+CGG + Chr4:42817876-42817895 None:intergenic 45.0%
ATAGTCCCCAACTTTACACG+GGG - Chr4:42820728-42820747 MsG0480020521.01.T01:intron 45.0%
CAACTCACATGCTTGAGCAT+TGG + Chr4:42821020-42821039 None:intergenic 45.0%
CAGATTATGGTGGTCCATTG+TGG + Chr4:42819453-42819472 None:intergenic 45.0%
CAGCAGCTACAGAGATGTAA+GGG + Chr4:42818697-42818716 None:intergenic 45.0%
CCGACACTCATACAAGACAA+AGG + Chr4:42818159-42818178 None:intergenic 45.0%
CTGCATCAGCATAAGCTTCT+AGG + Chr4:42820611-42820630 None:intergenic 45.0%
CTTCTGATTCTTCAGAAGGG+GGG + Chr4:42820991-42821010 None:intergenic 45.0%
GATAGTCCCCAACTTTACAC+GGG - Chr4:42820727-42820746 MsG0480020521.01.T01:intron 45.0%
GCCATCGTATCAGATTATGG+TGG + Chr4:42819526-42819545 None:intergenic 45.0%
GCCCAAGTCCAACGTATAAA+AGG + Chr4:42821212-42821231 None:intergenic 45.0%
GCGACGATAATGTCTAGACA+GGG - Chr4:42821230-42821249 MsG0480020521.01.T01:intron 45.0%
GCTACAGAGATGTAAGGGTT+CGG + Chr4:42818692-42818711 None:intergenic 45.0%
GCTCAAGCATGTGAGTTGTT+TGG - Chr4:42821022-42821041 MsG0480020521.01.T01:intron 45.0%
GGGAAAGGAATGAGAGGATT+TGG - Chr4:42820669-42820688 MsG0480020521.01.T01:intron 45.0%
GGGGGAAAGGAAGGAAAAAA+TGG - Chr4:42820691-42820710 MsG0480020521.01.T01:intron 45.0%
GGTTATCGTTACAAACGAGG+AGG - Chr4:42819272-42819291 MsG0480020521.01.T01:intron 45.0%
TACAAACGGGAACTTCTACG+AGG - Chr4:42820212-42820231 MsG0480020521.01.T01:intron 45.0%
TATAGGACACCAAGAATCCG+TGG + Chr4:42821099-42821118 None:intergenic 45.0%
TATCCTTTGTCGCTCCAACT+GGG - Chr4:42817890-42817909 MsG0480020521.01.T01:CDS 45.0%
TCAGCAGCTACAGAGATGTA+AGG + Chr4:42818698-42818717 None:intergenic 45.0%
TCCAATCCAAGTTTGCAGCT+CGG + Chr4:42820030-42820049 None:intergenic 45.0%
TCTCTTAAGAATCTCGCCGT+TGG + Chr4:42818928-42818947 None:intergenic 45.0%
TGCGATCAAAATCATGCTGG+CGG - Chr4:42819003-42819022 MsG0480020521.01.T01:intron 45.0%
TTATCCTTTGTCGCTCCAAC+TGG - Chr4:42817889-42817908 MsG0480020521.01.T01:CDS 45.0%
TTGTTCATGCCACGGATTCT+TGG - Chr4:42821087-42821106 MsG0480020521.01.T01:intron 45.0%
TTTGAGGGTAGGGGAGAAAA+GGG + Chr4:42820962-42820981 None:intergenic 45.0%
! AAATGCAAGACCAGGTTGAC+CGG - Chr4:42819966-42819985 MsG0480020521.01.T01:intron 45.0%
! AACTTGGTAATCCAGCCATC+TGG + Chr4:42817942-42817961 None:intergenic 45.0%
! ATGATGGCGTACAGCCAATA+TGG - Chr4:42819475-42819494 MsG0480020521.01.T01:intron 45.0%
! CAAATGGGGATTGCTGTTAC+AGG + Chr4:42818644-42818663 None:intergenic 45.0%
! CTCCTTTTGCCCTTCTACAA+TGG - Chr4:42821268-42821287 MsG0480020521.01.T01:intron 45.0%
! GGGAGTTTCTCACCTTGTTT+GGG + Chr4:42820931-42820950 None:intergenic 45.0%
!! AATCACTCCTTTTGGAGAGG+AGG - Chr4:42819755-42819774 MsG0480020521.01.T01:intron 45.0%
!! AGTCGAGTTAGCTTGCAACA+AGG - Chr4:42820358-42820377 MsG0480020521.01.T01:CDS 45.0%
!! ATCACTCCTTTTGGAGAGGA+GGG - Chr4:42819756-42819775 MsG0480020521.01.T01:intron 45.0%
!! CAAGAAGAGTATTCGAGAGG+TGG - Chr4:42820793-42820812 MsG0480020521.01.T01:intron 45.0%
!! CAGTCAATATGGCGGGATTT+TGG - Chr4:42819552-42819571 MsG0480020521.01.T01:intron 45.0%
!! GACAATCACTCCTTTTGGAG+AGG - Chr4:42819752-42819771 MsG0480020521.01.T01:intron 45.0%
!! GAGCTTTCTTCTCGATTTGG+TGG + Chr4:42818770-42818789 None:intergenic 45.0%
!! GCTGCAAATCCGTTTTGTTC+TGG - Chr4:42821482-42821501 MsG0480020521.01.T01:CDS 45.0%
!! TAATCTGGTTGGCTTGTGTC+TGG - Chr4:42817983-42818002 MsG0480020521.01.T01:intron 45.0%
!! TCTTCTCGATTTGGTGGAGT+AGG + Chr4:42818764-42818783 None:intergenic 45.0%
!! TTTTGAGGGTAGGGGAGAAA+AGG + Chr4:42820963-42820982 None:intergenic 45.0%
!!! GAGGTGGTTTTAGACGGATT+TGG - Chr4:42820809-42820828 MsG0480020521.01.T01:intron 45.0%
!!! GGGGTTTTGATTCTCCACAA+TGG - Chr4:42819436-42819455 MsG0480020521.01.T01:intron 45.0%
!! AAATTCAAATTTATTTAATT+AGG - Chr4:42818559-42818578 MsG0480020521.01.T01:intron 5.0%
!!! AAAAATTATTTTATATATCA+AGG + Chr4:42818398-42818417 None:intergenic 5.0%
!!! ATATAAAATAAATTTTTGAA+AGG - Chr4:42818425-42818444 MsG0480020521.01.T01:intron 5.0%
!!! ATATAAAATAATTTTTTTGA+AGG - Chr4:42818402-42818421 MsG0480020521.01.T01:intron 5.0%
AACACACCCTCCTCTCCAAA+AGG + Chr4:42819765-42819784 None:intergenic 50.0%
AGGAAGGCGATTGAAGGAAG+GGG - Chr4:42817811-42817830 MsG0480020521.01.T01:CDS 50.0%
AGGATTGTTCTGTGTGGTGG+TGG - Chr4:42817836-42817855 MsG0480020521.01.T01:CDS 50.0%
ATCCAAACGTACCCCTTCAC+CGG + Chr4:42819037-42819056 None:intergenic 50.0%
ATGAGAGGATTTGGGGGGAA+AGG - Chr4:42820678-42820697 MsG0480020521.01.T01:intron 50.0%
ATGGCGCCACACAGTCAATA+TGG - Chr4:42819541-42819560 MsG0480020521.01.T01:intron 50.0%
ATGGCGTACAGCCAATATGG+CGG - Chr4:42819478-42819497 MsG0480020521.01.T01:intron 50.0%
CTTTCTTCAGTTCGGCCAGA+TGG - Chr4:42817924-42817943 MsG0480020521.01.T01:intron 50.0%
GAGCCAAAATCCCGCCATAT+TGG + Chr4:42819492-42819511 None:intergenic 50.0%
GAGGAAGGCGATTGAAGGAA+GGG - Chr4:42817810-42817829 MsG0480020521.01.T01:CDS 50.0%
GATATGACAGTGCCACAAGG+TGG - Chr4:42819403-42819422 MsG0480020521.01.T01:CDS 50.0%
GATTCTTCAGAAGGGGGGAA+TGG + Chr4:42820986-42821005 None:intergenic 50.0%
GGAAGGATTGTTCTGTGTGG+TGG - Chr4:42817833-42817852 MsG0480020521.01.T01:CDS 50.0%
GGCACTGTCATATCGGACTA+TGG + Chr4:42819397-42819416 None:intergenic 50.0%
GGCGACGATAATGTCTAGAC+AGG - Chr4:42821229-42821248 MsG0480020521.01.T01:intron 50.0%
GGCGCCATCGTATCAGATTA+TGG + Chr4:42819529-42819548 None:intergenic 50.0%
GGCGGAAAATGTGGCCATAT+CGG + Chr4:42819376-42819395 None:intergenic 50.0%
GGGCTCAATTTCAGCCGATA+TGG - Chr4:42819359-42819378 MsG0480020521.01.T01:CDS 50.0%
GGGGAGTTTCTCACCTTGTT+TGG + Chr4:42820932-42820951 None:intergenic 50.0%
GGTAGGGGAGAAAAGGGAAA+GGG + Chr4:42820956-42820975 None:intergenic 50.0%
TCCGATATGACAGTGCCACA+AGG - Chr4:42819400-42819419 MsG0480020521.01.T01:CDS 50.0%
TGCTCACCATGCTTGTAGTG+AGG - Chr4:42820057-42820076 MsG0480020521.01.T01:intron 50.0%
TTAGCTCGTGCACTATCACC+CGG + Chr4:42819988-42820007 None:intergenic 50.0%
TTGGGCATGGCAGATATTGG+TGG - Chr4:42819328-42819347 MsG0480020521.01.T01:CDS 50.0%
! AATGCAAGACCAGGTTGACC+GGG - Chr4:42819967-42819986 MsG0480020521.01.T01:intron 50.0%
! ATCCCGCCATATTGACTGTG+TGG + Chr4:42819550-42819569 None:intergenic 50.0%
! CAACGACACTGGTCTAACCA+AGG - Chr4:42819603-42819622 MsG0480020521.01.T01:intron 50.0%
! GGGTGTGTTGTTCCTTACGA+TGG - Chr4:42819776-42819795 MsG0480020521.01.T01:intron 50.0%
! GGTTAGACCAGTGTCGTTGA+TGG + Chr4:42819602-42819621 None:intergenic 50.0%
! TCGGACTATGGCGGAAAATG+TGG + Chr4:42819385-42819404 None:intergenic 50.0%
!! AGAAGGGGGGAATGGTTTTG+AGG + Chr4:42820978-42820997 None:intergenic 50.0%
!! CAGCCAATATGGCGGGATTT+TGG - Chr4:42819486-42819505 MsG0480020521.01.T01:intron 50.0%
!!! GAAGGGGGGAATGGTTTTGA+GGG + Chr4:42820977-42820996 None:intergenic 50.0%
!!! GGGAATGGTTTTGAGGGTAG+GGG + Chr4:42820971-42820990 None:intergenic 50.0%
!!! GGGGAATGGTTTTGAGGGTA+GGG + Chr4:42820972-42820991 None:intergenic 50.0%
ACTCCCAGTTGGAGCGACAA+AGG + Chr4:42817896-42817915 None:intergenic 55.0%
AGGGGAGAAAAGGGAAAGGG+AGG + Chr4:42820953-42820972 None:intergenic 55.0%
CATGCTGGCGGTTATGTTCC+CGG - Chr4:42819015-42819034 MsG0480020521.01.T01:intron 55.0%
CCATCTGCCATCAACGACAC+TGG - Chr4:42819592-42819611 MsG0480020521.01.T01:intron 55.0%
CGCCACACAGTCAATATGGC+GGG - Chr4:42819545-42819564 MsG0480020521.01.T01:intron 55.0%
CGGTTATGTTCCCGGTGAAG+GGG - Chr4:42819023-42819042 MsG0480020521.01.T01:intron 55.0%
GAGAGGAGGAAGGCGATTGA+AGG - Chr4:42817805-42817824 MsG0480020521.01.T01:CDS 55.0%
GAGGATTTGGGGGGAAAGGA+AGG - Chr4:42820682-42820701 MsG0480020521.01.T01:intron 55.0%
GCCGAGCTGCAAACTTGGAT+TGG - Chr4:42820026-42820045 MsG0480020521.01.T01:intron 55.0%
GCGCCACACAGTCAATATGG+CGG - Chr4:42819544-42819563 MsG0480020521.01.T01:intron 55.0%
GCGGTTATGTTCCCGGTGAA+GGG - Chr4:42819022-42819041 MsG0480020521.01.T01:intron 55.0%
GGAAGGCGATTGAAGGAAGG+GGG - Chr4:42817812-42817831 MsG0480020521.01.T01:CDS 55.0%
GGAGGAAGGCGATTGAAGGA+AGG - Chr4:42817809-42817828 MsG0480020521.01.T01:CDS 55.0%
GGGAGAAAAGGGAAAGGGAG+GGG + Chr4:42820951-42820970 None:intergenic 55.0%
GGGGAGAAAAGGGAAAGGGA+GGG + Chr4:42820952-42820971 None:intergenic 55.0%
GGGGGAAGGATTGTTCTGTG+TGG - Chr4:42817830-42817849 MsG0480020521.01.T01:CDS 55.0%
GGGTAGGGGAGAAAAGGGAA+AGG + Chr4:42820957-42820976 None:intergenic 55.0%
TCCAAACGTACCCCTTCACC+GGG + Chr4:42819036-42819055 None:intergenic 55.0%
TGACAGTGCCACAAGGTGGT+TGG - Chr4:42819407-42819426 MsG0480020521.01.T01:intron 55.0%
TGACTGCCGAGCTGCAAACT+TGG - Chr4:42820021-42820040 MsG0480020521.01.T01:intron 55.0%
TGGCGTACAGCCAATATGGC+GGG - Chr4:42819479-42819498 MsG0480020521.01.T01:intron 55.0%
TTGTCGCTCCAACTGGGAGT+AGG - Chr4:42817896-42817915 MsG0480020521.01.T01:CDS 55.0%
! ACAAGGTGGTTGGTATGGCG+GGG - Chr4:42819417-42819436 MsG0480020521.01.T01:intron 55.0%
! ATGATGCAGGGTATCCGTGG+TGG - Chr4:42817859-42817878 MsG0480020521.01.T01:CDS 55.0%
! CACAAGGTGGTTGGTATGGC+GGG - Chr4:42819416-42819435 MsG0480020521.01.T01:intron 55.0%
! CCACAAGGTGGTTGGTATGG+CGG - Chr4:42819415-42819434 MsG0480020521.01.T01:intron 55.0%
! GTGCCACAAGGTGGTTGGTA+TGG - Chr4:42819412-42819431 MsG0480020521.01.T01:intron 55.0%
! TGATGGCAGATGGTGGTCCA+TGG + Chr4:42819585-42819604 None:intergenic 55.0%
! TGGATGATGCAGGGTATCCG+TGG - Chr4:42817856-42817875 MsG0480020521.01.T01:CDS 55.0%
!! CCAGTGTCGTTGATGGCAGA+TGG + Chr4:42819595-42819614 None:intergenic 55.0%
!! GTGTCGTTGATGGCAGATGG+TGG + Chr4:42819592-42819611 None:intergenic 55.0%
!! GTGTGGTGGTGGATGATGCA+GGG - Chr4:42817847-42817866 MsG0480020521.01.T01:CDS 55.0%
!! TGTGTGGTGGTGGATGATGC+AGG - Chr4:42817846-42817865 MsG0480020521.01.T01:CDS 55.0%
!!! GGGATTTTGGCTCTCTGCCA+TGG - Chr4:42819499-42819518 MsG0480020521.01.T01:intron 55.0%
!!! GGGGGAATGGTTTTGAGGGT+AGG + Chr4:42820973-42820992 None:intergenic 55.0%
CCGCCATACCAACCACCTTG+TGG + Chr4:42819418-42819437 None:intergenic 60.0%
CTTCAGTTCGGCCAGATGGC+TGG - Chr4:42817928-42817947 MsG0480020521.01.T01:intron 60.0%
GCACTATCACCCGGTCAACC+TGG + Chr4:42819979-42819998 None:intergenic 60.0%
GGCGATTGAAGGAAGGGGGA+AGG - Chr4:42817816-42817835 MsG0480020521.01.T01:CDS 60.0%
GGCGGTTATGTTCCCGGTGA+AGG - Chr4:42819021-42819040 MsG0480020521.01.T01:intron 60.0%
! CAGGGTATCCGTGGTGGTGT+TGG - Chr4:42817865-42817884 MsG0480020521.01.T01:CDS 60.0%
! TCCCGGTGAAGGGGTACGTT+TGG - Chr4:42819032-42819051 MsG0480020521.01.T01:intron 60.0%
! TGGCAGATGGTGGTCCATGG+CGG + Chr4:42819582-42819601 None:intergenic 60.0%
!!! GGGATTTTGGCTCTCCGCCA+TGG - Chr4:42819565-42819584 MsG0480020521.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 42817802 42821583 42817802 ID=MsG0480020521.01;Name=MsG0480020521.01
Chr4 mRNA 42817802 42821583 42817802 ID=MsG0480020521.01.T01;Parent=MsG0480020521.01;Name=MsG0480020521.01.T01;_AED=0.43;_eAED=0.45;_QI=0|0.25|0.4|1|1|1|5|0|184
Chr4 exon 42821472 42821583 42821472 ID=MsG0480020521.01.T01:exon:10675;Parent=MsG0480020521.01.T01
Chr4 exon 42820341 42820462 42820341 ID=MsG0480020521.01.T01:exon:10676;Parent=MsG0480020521.01.T01
Chr4 exon 42819307 42819423 42819307 ID=MsG0480020521.01.T01:exon:10677;Parent=MsG0480020521.01.T01
Chr4 exon 42819123 42819206 42819123 ID=MsG0480020521.01.T01:exon:10678;Parent=MsG0480020521.01.T01
Chr4 exon 42817802 42817921 42817802 ID=MsG0480020521.01.T01:exon:10679;Parent=MsG0480020521.01.T01
Chr4 CDS 42821472 42821583 42821472 ID=MsG0480020521.01.T01:cds;Parent=MsG0480020521.01.T01
Chr4 CDS 42820341 42820462 42820341 ID=MsG0480020521.01.T01:cds;Parent=MsG0480020521.01.T01
Chr4 CDS 42819307 42819423 42819307 ID=MsG0480020521.01.T01:cds;Parent=MsG0480020521.01.T01
Chr4 CDS 42819123 42819206 42819123 ID=MsG0480020521.01.T01:cds;Parent=MsG0480020521.01.T01
Chr4 CDS 42817802 42817921 42817802 ID=MsG0480020521.01.T01:cds;Parent=MsG0480020521.01.T01
Gene Sequence

>MsG0480020521.01.T01

ATGGAGAGGAGGAAGGCGATTGAAGGAAGGGGGAAGGATTGTTCTGTGTGGTGGTGGATGATGCAGGGTATCCGTGGTGGTGTTGGTTTATCCTTTGTCGCTCCAACTGGGACTCCAACGGCGAGATTCTTAAGAGAACATTTGGAGAAAGCTGGGTGTCCTGTTCAAGATAATTTCTTCAAAGCTATTAATTGCGATCAAAATCATGCTGGCGGTTATGTTCCCGGTGAAGGGATAGAAATGCAAGACCAGGTTGACCGGGTGATAGTGCACGAGCTAATTCATGTATTTGATGACTGCCGAGCTGCAAACTTGGATTGGACTGATTGTGCTCACCATGCTTGTAGTGAGATAAGAGCTGGTCATCTAAGTGGTGATTGTCATTACAAACGGGAACTTCTACGAGGACATTTGAAAATACGAGGGCAAGAACAAGAGGGTTTTAACATCATGGCTGCAAATCCGTTTTGTTCTGGTTCAACTGCAAAGAATGCTATGGAAGCTGTATGGGAAACTTGTTATAACGATACAGCGCCTTTTGATAGAGCTCCTTAA
Protein sequence

>MsG0480020521.01.T01

MERRKAIEGRGKDCSVWWWMMQGIRGGVGLSFVAPTGTPTARFLREHLEKAGCPVQDNFFKAINCDQNHAGGYVPGEGIEMQDQVDRVIVHELIHVFDDCRAANLDWTDCAHHACSEIRAGHLSGDCHYKRELLRGHLKIRGQEQEGFNIMAANPFCSGSTAKNAMEAVWETCYNDTAPFDRAP*