AlfalfaGEDB | MsG0180000546.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000546.01

KEGG: K17499
GO: GO:0003824
PFAM: PF00481

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000546.01.T01	GAU17699.1	64.184	282	48	4	9	241	1	278	6.70E-115	343

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000546.01.T01	Q9SZ53	54.839	279	74	4	11	241	1	275	4.03E-96	288

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000546.01.T01	G7I890	66.436	289	43	5	1	241	31	313	2.64e-120	357

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048761.01	MsG0180000546.01	-0.805518	1.284028e-49	6.806964e-47
MsG0180000152.01	MsG0180000546.01	0.830107	3.537511e-55	3.691460e-52
MsG0180000240.01	MsG0180000546.01	-0.813942	1.982829e-51	1.312600e-48
MsG0180000271.01	MsG0180000546.01	0.826394	2.779149e-54	2.603120e-51
MsG0180000546.01	MsG0180000550.01	0.854708	1.028291e-61	2.317086e-58
MsG0180000546.01	MsG0180000669.01	0.858296	9.081095e-63	2.306922e-59
MsG0180000546.01	MsG0180000679.01	0.828816	7.284471e-55	7.320269e-52
MsG0180000546.01	MsG0180000680.01	0.817342	3.465463e-52	2.517089e-49
MsG0180000546.01	MsG0180001141.01	0.824480	7.891489e-54	6.998159e-51
MsG0180000546.01	MsG0180001213.01	0.823612	1.260992e-53	1.091134e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000546.01.T01	MTR_1g014190	66.436	289	43	5	1	241	31	313	6.69e-124	357
MsG0180000546.01.T01	MTR_1g014190	67.384	279	39	4	11	241	1	275	1.00e-122	353
MsG0180000546.01.T01	MTR_3g105880	57.348	279	67	4	11	241	1	275	8.67e-103	303
MsG0180000546.01.T01	MTR_3g105880	57.348	279	67	4	11	241	1	275	3.60e-102	300
MsG0180000546.01.T01	MTR_4g007440	52.500	280	79	5	11	241	1	275	5.72e-91	273
MsG0180000546.01.T01	MTR_6g081850	36.181	199	98	8	62	241	80	268	4.52e-24	98.6
MsG0180000546.01.T01	MTR_4g123080	30.846	201	99	5	62	241	96	277	5.40e-22	93.6
MsG0180000546.01.T01	MTR_4g123080	30.846	201	99	5	62	241	114	295	7.04e-22	93.6
MsG0180000546.01.T01	MTR_5g024340	30.208	192	102	5	62	241	125	296	5.43e-21	90.9
MsG0180000546.01.T01	MTR_5g009370	33.831	201	90	7	62	241	88	266	1.08e-20	89.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000546.01.T01	AT4G31860	54.839	279	74	4	11	241	1	275	1.17e-97	286
MsG0180000546.01.T01	AT4G31860	54.839	279	74	4	11	241	1	275	1.88e-97	287
MsG0180000546.01.T01	AT4G31860	54.839	279	74	4	11	241	1	275	4.11e-97	288
MsG0180000546.01.T01	AT2G25070	54.480	279	75	4	11	241	1	275	8.10e-97	287
MsG0180000546.01.T01	AT2G25070	54.480	279	75	4	11	241	1	275	8.10e-97	287
MsG0180000546.01.T01	AT2G25620	31.282	195	103	7	62	241	129	307	3.45e-20	89.0
MsG0180000546.01.T01	AT2G25620	31.282	195	103	7	62	241	129	307	3.45e-20	89.0
MsG0180000546.01.T01	AT1G18030	32.338	201	103	8	62	241	110	298	8.32e-19	84.0
MsG0180000546.01.T01	AT1G18030	32.338	201	103	8	62	241	110	298	1.08e-18	84.3
MsG0180000546.01.T01	AT3G51470	28.646	192	105	5	62	241	63	234	4.77e-18	82.0

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Find 45 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTACAATGTTTATGTC+TGG	0.447995	1:+7658980	None:intergenic
AAAGCAGTGCTTCCTGAAGT+TGG	0.515324	1:+7659838	None:intergenic
AAGAGTGAAGAATACATAGC+TGG	0.546958	1:-7660350	MsG0180000546.01.T01:CDS
AAGGAAAGAATCTATAAAGC+TGG	0.515169	1:-7659414	MsG0180000546.01.T01:CDS
AATGTTTATGTCTGGATGGG+CGG	0.577884	1:+7658988	None:intergenic
ACCTGAGCTTGTGATTGAGA+AGG	0.615530	1:-7659433	MsG0180000546.01.T01:CDS
AGAATGGATGAGATGATGCG+TGG	0.759110	1:-7660128	MsG0180000546.01.T01:CDS
ATATGTTTATCATCTATGCA+AGG	0.621432	1:-7661051	MsG0180000546.01.T01:CDS
ATGAGATGATGCGTGGTCAA+AGG	0.604481	1:-7660121	MsG0180000546.01.T01:CDS
ATTAGCAGTCTTAGGTGATA+AGG	0.484258	1:-7660087	MsG0180000546.01.T01:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGCTTAGTCAAGAGTTT+CGG	-	Chr1:7661326-7661345	MsG0180000546.01.T01:five_prime_UTR	30.0%
AAGGAAAGAATCTATAAAGC+TGG	-	Chr1:7660809-7660828	MsG0180000546.01.T01:intron	30.0%
ACTAAGATTATCATAGCTCA+AGG	+	Chr1:7660972-7660991	None:intergenic	30.0%
ATTGTAGAAGCAAGTATATG+TGG	-	Chr1:7659325-7659344	MsG0180000546.01.T01:intron	30.0%
CAACCATATACAACAAAACA+TGG	+	Chr1:7658851-7658870	None:intergenic	30.0%
CTTAGTGTACCGAAAATAAA+AGG	-	Chr1:7660986-7661005	MsG0180000546.01.T01:intron	30.0%
GCCAAATCAATTAACAAAAG+AGG	+	Chr1:7658966-7658985	None:intergenic	30.0%
GGAATGATTGAAGATTGATT+CGG	-	Chr1:7660173-7660192	MsG0180000546.01.T01:intron	30.0%
TACAATGTTTATGTCTGGAT+GGG	+	Chr1:7661241-7661260	None:intergenic	30.0%
TCCTTATTATCATTGCTTCT+GGG	+	Chr1:7660202-7660221	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	7658713	7661532	7658713	ID=MsG0180000546.01;Name=MsG0180000546.01
Chr1	mRNA	7658713	7661532	7658713	ID=MsG0180000546.01.T01;Parent=MsG0180000546.01;Name=MsG0180000546.01.T01;_AED=0.49;_eAED=0.50;_QI=361\|0.71\|0.62\|1\|1\|1\|8\|0\|246
Chr1	exon	7661026	7661532	7661026	ID=MsG0180000546.01.T01:exon:2852;Parent=MsG0180000546.01.T01
Chr1	exon	7660511	7660572	7660511	ID=MsG0180000546.01.T01:exon:2853;Parent=MsG0180000546.01.T01
Chr1	exon	7660314	7660422	7660314	ID=MsG0180000546.01.T01:exon:2854;Parent=MsG0180000546.01.T01
Chr1	exon	7660088	7660148	7660088	ID=MsG0180000546.01.T01:exon:2855;Parent=MsG0180000546.01.T01
Chr1	exon	7659795	7659884	7659795	ID=MsG0180000546.01.T01:exon:2856;Parent=MsG0180000546.01.T01
Chr1	exon	7659358	7659481	7659358	ID=MsG0180000546.01.T01:exon:2857;Parent=MsG0180000546.01.T01
Chr1	exon	7658988	7659064	7658988	ID=MsG0180000546.01.T01:exon:2858;Parent=MsG0180000546.01.T01
Chr1	exon	7658713	7658784	7658713	ID=MsG0180000546.01.T01:exon:2859;Parent=MsG0180000546.01.T01
Chr1	five_prime_UTR	7661172	7661532	7661172	ID=MsG0180000546.01.T01:five_prime_utr;Parent=MsG0180000546.01.T01
Chr1	CDS	7661026	7661171	7661026	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7660511	7660572	7660511	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7660314	7660422	7660314	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7660088	7660148	7660088	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7659795	7659884	7659795	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7659358	7659481	7659358	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7658988	7659064	7658988	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01
Chr1	CDS	7658713	7658784	7658713	ID=MsG0180000546.01.T01:cds;Parent=MsG0180000546.01.T01

Gene Sequence

>MsG0180000546.01.T01

ATGCTTATTTGTTTTAATTCAGATATAGCAATGGGAACAACTCTCAGCATTCCTAAAACTGAAAAGTTTTCAGAAGATGGTGAAAATGACAATCTTAGATATGTTTATCATCTATGCAAGGTTGGCGTGCAACAATGGAAGATGCACATGCGGCGCATCTTGATGTGGATTCATCCACTTCGTTTTTCGGTATATGATGGTCATGGAGGTAAGGCGGTTGCAAAGTTTTGTGCCAAGCACCTTCACCAACAGGTGCTCAAGAGTGAAGAATACATAGCTGGAGATGTTGGAACATCTCTTACGAAAGCATTTTTCAGAATGGATGAGATGATGCGTGGTCAAAGGGGATGGAGGGAATTAGCAGTCTTAGGTGATAAGGGGCCTCATTCAGACTTTGATGGACCAACTTCAGGAAGCACTGCTTTGTTGCAATCATTAGAAACAACCTACTTTTTGTTGCGAATGCTGGCATACAATTTATCTAGAGATCACAAACCTGAGCTTGTGATTGAGAAGGAAAGAATCTATAAAGCTGGTGGTTTTATTCATGCAGGACGAATTAATGGGAGTTTAAATCTTGCAAGAGCTATAGGTGACGTGGACTTCAAAAATAATAGATTTCTTTCTGCTGAAAAACAAGTTGTGACCGCCCATCCAGACATAAACATTGTTGACCTTCATGACGAAGATGAGTTTATAGTGATAGCTTGTGATGGCATATGGTATGGTTCTTATGCTTGA

Protein sequence

>MsG0180000546.01.T01

MLICFNSDIAMGTTLSIPKTEKFSEDGENDNLRYVYHLCKVGVQQWKMHMRRILMWIHPLRFSVYDGHGGKAVAKFCAKHLHQQVLKSEEYIAGDVGTSLTKAFFRMDEMMRGQRGWRELAVLGDKGPHSDFDGPTSGSTALLQSLETTYFLLRMLAYNLSRDHKPELVIEKERIYKAGGFIHAGRINGSLNLARAIGDVDFKNNRFLSAEKQVVTAHPDINIVDLHDEDEFIVIACDGIWYGSYA*