AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001401.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCATCTGGCTTTGTATTT+TGG 0.128532 1:+20585446 MsG0180001401.01.T01:CDS
AAAATACTGACAAGTATTTA+AGG 0.157158 1:+20585191 MsG0180001401.01.T01:CDS
ACTTATTCTCATTTGATATA+TGG 0.213441 1:+20585040 MsG0180001401.01.T01:CDS
ACTTCTCTCTTTCGGATAAA+AGG 0.252828 1:+20586062 MsG0180001401.01.T01:CDS
ACATATATACTTCTCTCTTT+CGG 0.366382 1:+20586054 MsG0180001401.01.T01:CDS
GACTATGAAAGGTCGATTGC+TGG 0.402524 1:+20585213 MsG0180001401.01.T01:CDS
ATCAACTCGCGTTTATCATC+TGG 0.409289 1:+20585433 MsG0180001401.01.T01:CDS
CTGTTATTGAGTGGAGTTTG+TGG 0.429531 1:+20587952 MsG0180001401.01.T01:CDS
AGATGATAAACGCGAGTTGA+TGG 0.432933 1:-20585431 None:intergenic
TGACTGTGTTGTTAGATAAA+GGG 0.467170 1:+20584999 MsG0180001401.01.T01:intron
CCACTGATATGACTCTATCT+TGG 0.477722 1:-20586018 None:intergenic
TGGCTATGTGAGAAATGTCC+AGG 0.500347 1:+20585060 MsG0180001401.01.T01:CDS
ATTATGCTTTACAGTTCAAG+TGG 0.523124 1:+20587990 MsG0180001401.01.T01:CDS
GATGATAAACGCGAGTTGAT+GGG 0.529535 1:-20585430 None:intergenic
GTCTTCACAAATCAATTCAG+AGG 0.559552 1:+20582506 MsG0180001401.01.T01:CDS
GTCTTCACAAATCAATTCAG+AGG 0.559552 1:+20585922 MsG0180001401.01.T01:CDS
GAAGGTTGTCTGTTATTGAG+TGG 0.594990 1:+20587943 MsG0180001401.01.T01:intron
TGAGAATAAGTAACATCACT+TGG 0.601816 1:-20585028 None:intergenic
TTATTGAGTGGAGTTTGTGG+TGG 0.605894 1:+20587955 MsG0180001401.01.T01:CDS
AAGTATTTAAGGACTATGAA+AGG 0.617133 1:+20585202 MsG0180001401.01.T01:CDS
CCAAGATAGAGTCATATCAG+TGG 0.675812 1:+20586018 MsG0180001401.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATTAAATGTTAGTTA+TGG + Chr1:20582683-20582702 MsG0180001401.01.T01:intron 10.0%
!!! AAATTTTATTCTATTCTTAA+AGG - Chr1:20587781-20587800 None:intergenic 10.0%
!!! TTTTATTGATTATTTATTTC+GGG - Chr1:20584099-20584118 None:intergenic 10.0%
!!! TTTTTTGGATTTTATAATTT+AGG + Chr1:20587465-20587484 MsG0180001401.01.T01:intron 10.0%
!! ACGGAAAAATTAATATTAAT+GGG - Chr1:20584474-20584493 None:intergenic 15.0%
!! ATGAATAATTTAGTCATAAA+AGG - Chr1:20587345-20587364 None:intergenic 15.0%
!! TACGGAAAAATTAATATTAA+TGG - Chr1:20584475-20584494 None:intergenic 15.0%
!! TTTACTTAAAATCCAATATA+AGG - Chr1:20587108-20587127 None:intergenic 15.0%
!! TTTACTTAAAATCGAATATA+AGG - Chr1:20583730-20583749 None:intergenic 15.0%
!!! AGAAATTATTTTCTTTAGTT+TGG - Chr1:20587745-20587764 None:intergenic 15.0%
!! AAAATACTGACAAGTATTTA+AGG + Chr1:20585191-20585210 MsG0180001401.01.T01:CDS 20.0%
!! AAACGTTATTCTATTCTTAA+AGG - Chr1:20584232-20584251 None:intergenic 20.0%
!! AAATATAAGTTTCACAACTA+AGG - Chr1:20584003-20584022 None:intergenic 20.0%
!! AAATCCAAAAAAAATCTTCA+AGG - Chr1:20587457-20587476 None:intergenic 20.0%
!! ACTTATTCTCATTTGATATA+TGG + Chr1:20585040-20585059 MsG0180001401.01.T01:CDS 20.0%
!! ATTTATCTCATATCATACAT+AGG + Chr1:20583645-20583664 MsG0180001401.01.T01:intron 20.0%
!! TCTGAAGATATATTATGAAA+TGG + Chr1:20585093-20585112 MsG0180001401.01.T01:intron 20.0%
!!! AAGGTTTTTCAAAATAAGTA+TGG + Chr1:20584287-20584306 MsG0180001401.01.T01:intron 20.0%
!!! ATTCTAATGTTTTAAACTCT+TGG - Chr1:20586235-20586254 None:intergenic 20.0%
!!! CAATAGGAATTTTATAATGA+AGG - Chr1:20584200-20584219 None:intergenic 20.0%
!!! GATAATTGTTTTGAAGATTA+TGG + Chr1:20582465-20582484 MsG0180001401.01.T01:CDS 20.0%
!!! GATGATTCTGTTTTTTTTTA+AGG + Chr1:20585859-20585878 MsG0180001401.01.T01:intron 20.0%
!!! GTTTTAAAACTTCTTTATCT+TGG - Chr1:20583316-20583335 None:intergenic 20.0%
!!! TAGTTGTGAAACTTATATTT+TGG + Chr1:20584003-20584022 MsG0180001401.01.T01:intron 20.0%
!!! TATTCCTTGAAGATTTTTTT+TGG + Chr1:20587450-20587469 MsG0180001401.01.T01:intron 20.0%
!!! TATTGGATTTTAAGTAAACT+TGG + Chr1:20587110-20587129 MsG0180001401.01.T01:intron 20.0%
!!! TTGTTAATTTGAGATTGATA+TGG + Chr1:20584885-20584904 MsG0180001401.01.T01:intron 20.0%
! AAGTATTTAAGGACTATGAA+AGG + Chr1:20585202-20585221 MsG0180001401.01.T01:CDS 25.0%
! AATCTTCAAGGAATAAACAT+AGG - Chr1:20587445-20587464 None:intergenic 25.0%
! AATGTCTAAATTAATGCAAG+AGG - Chr1:20584629-20584648 None:intergenic 25.0%
! AATTCACTTCAAATACCAAA+CGG - Chr1:20582731-20582750 None:intergenic 25.0%
! ACATATATACTTCTCTCTTT+CGG + Chr1:20582638-20582657 MsG0180001401.01.T01:intron 25.0%
! ACTATAAAGTTTGATTGCAT+TGG - Chr1:20586370-20586389 None:intergenic 25.0%
! ATAAACATAGGTAATTTAGC+AGG - Chr1:20583979-20583998 None:intergenic 25.0%
! ATGGTTACAAAATTCAAATG+AGG + Chr1:20587178-20587197 MsG0180001401.01.T01:intron 25.0%
! ATTGAAAAGATGTTCTTTGA+GGG - Chr1:20583510-20583529 None:intergenic 25.0%
! GAATTTACAGCTTAATCTTA+AGG + Chr1:20586761-20586780 MsG0180001401.01.T01:intron 25.0%
! GAATTTACCTCTTAATCTTA+AGG + Chr1:20583383-20583402 MsG0180001401.01.T01:intron 25.0%
! GACTAATAAACAGATAAACT+AGG - Chr1:20585136-20585155 None:intergenic 25.0%
! TAAAAGTGACAATAAGAACT+AGG + Chr1:20587510-20587529 MsG0180001401.01.T01:intron 25.0%
! TCAATCAACAATTTCTTACT+GGG - Chr1:20587719-20587738 None:intergenic 25.0%
! TCATTATCCAAATTCACTTA+AGG + Chr1:20585726-20585745 MsG0180001401.01.T01:intron 25.0%
! TCTGAAGAAGATATAAGATA+AGG - Chr1:20587300-20587319 None:intergenic 25.0%
! TTCAATCAACAATTTCTTAC+TGG - Chr1:20587720-20587739 None:intergenic 25.0%
! TTGAAACTTTGCATACAATA+TGG + Chr1:20585837-20585856 MsG0180001401.01.T01:intron 25.0%
!! ATTCTAATGTTTTACACTCT+TGG - Chr1:20582819-20582838 None:intergenic 25.0%
!! GGTGAATTGTAATTTTGTAT+CGG + Chr1:20584694-20584713 MsG0180001401.01.T01:intron 25.0%
!! TAATTTGCCTTAAGTGAATT+TGG - Chr1:20585736-20585755 None:intergenic 25.0%
!! TATTTTCAACCTTTCCAAAA+CGG - Chr1:20585177-20585196 None:intergenic 25.0%
!! TTTTCAATGTTGTAAGTTTC+TGG + Chr1:20583522-20583541 MsG0180001401.01.T01:intron 25.0%
!!! ACTTCATGAATTTGTCATTT+TGG - Chr1:20586584-20586603 None:intergenic 25.0%
!!! ATATTTTTTATGCTTCCCAA+CGG - Chr1:20583886-20583905 None:intergenic 25.0%
!!! GAATAGAATAACGTTTTAGA+TGG + Chr1:20584236-20584255 MsG0180001401.01.T01:intron 25.0%
!!! GTTACTGAGATTTTTTTCAT+AGG + Chr1:20585768-20585787 MsG0180001401.01.T01:intron 25.0%
!!! TACATTGTTAATTTGTGAGA+AGG + Chr1:20584964-20584983 MsG0180001401.01.T01:intron 25.0%
!!! TACTAAGTTTTAAGCAAGTT+CGG + Chr1:20585400-20585419 MsG0180001401.01.T01:CDS 25.0%
!!! TATTCGATTTTAAGTAAACC+TGG + Chr1:20583732-20583751 MsG0180001401.01.T01:intron 25.0%
!!! TTATTTTGAAAAACCTTGTC+TGG - Chr1:20584284-20584303 None:intergenic 25.0%
!!! TTGTTCTTCTTTGTTTTATC+TGG + Chr1:20585637-20585656 MsG0180001401.01.T01:intron 25.0%
AAACAAAGAAGAACAAAACC+CGG - Chr1:20585633-20585652 None:intergenic 30.0%
AAACTTCTTTATCTTGGTCA+AGG - Chr1:20583310-20583329 None:intergenic 30.0%
AAGCCAAATATACTTTCATG+TGG + Chr1:20584118-20584137 MsG0180001401.01.T01:intron 30.0%
AAGGGAAAGATTGTGAAAAA+TGG + Chr1:20586431-20586450 MsG0180001401.01.T01:intron 30.0%
AATTTCAACAAGGAATGATG+GGG + Chr1:20587045-20587064 MsG0180001401.01.T01:intron 30.0%
AGCCAAATATACTTTCATGT+GGG + Chr1:20584119-20584138 MsG0180001401.01.T01:intron 30.0%
AGCCAATCATCAATGTTTAA+TGG - Chr1:20587607-20587626 None:intergenic 30.0%
AGTTAACAAGTGCCTTATAT+TGG + Chr1:20587093-20587112 MsG0180001401.01.T01:intron 30.0%
ATCGAAAAGATGTTCTTTGA+GGG - Chr1:20586887-20586906 None:intergenic 30.0%
ATGAAAATGCTTGTGCAAAA+AGG - Chr1:20587583-20587602 None:intergenic 30.0%
ATTATGCTTAACAGTTCAAG+TGG + Chr1:20584446-20584465 MsG0180001401.01.T01:intron 30.0%
ATTATGCTTTACAGTTCAAG+TGG + Chr1:20587990-20588009 MsG0180001401.01.T01:CDS 30.0%
CACAACTAAGGAATAAACAT+AGG - Chr1:20583991-20584010 None:intergenic 30.0%
CATACATAGGAATTTCAACA+AGG + Chr1:20587035-20587054 MsG0180001401.01.T01:intron 30.0%
CATTGAAAAGATGTTCTTTG+AGG - Chr1:20583511-20583530 None:intergenic 30.0%
CATTTGATTGAATTGAACCT+TGG + Chr1:20585668-20585687 MsG0180001401.01.T01:intron 30.0%
CTCAAATTAACAATAACCGA+AGG - Chr1:20584878-20584897 None:intergenic 30.0%
CTCTATGAGTGAAAATTCTA+CGG - Chr1:20584493-20584512 None:intergenic 30.0%
GAATTTCAACAAGGAATGAT+GGG + Chr1:20587044-20587063 MsG0180001401.01.T01:intron 30.0%
GTCTCATATATACTCACATT+TGG - Chr1:20585241-20585260 None:intergenic 30.0%
GTCTTTACACTTCTTTATCT+TGG - Chr1:20586693-20586712 None:intergenic 30.0%
GTTTATTGAGTTACTGTTGA+AGG + Chr1:20584379-20584398 MsG0180001401.01.T01:intron 30.0%
TAAGAGGTAAATTCTTGAGT+AGG - Chr1:20583377-20583396 None:intergenic 30.0%
TAAGCTGTAAATTCTTGAGT+AGG - Chr1:20586755-20586774 None:intergenic 30.0%
TAGCTAAATAGATAACCTTG+TGG + Chr1:20582883-20582902 MsG0180001401.01.T01:intron 30.0%
TCAAATACCTCAATAAGTCT+AGG - Chr1:20586608-20586627 None:intergenic 30.0%
TCATACATAGGATTTCAACA+AGG + Chr1:20583657-20583676 MsG0180001401.01.T01:intron 30.0%
TGAAAAACCTTGTCTGATTT+AGG - Chr1:20584278-20584297 None:intergenic 30.0%
TGAACAACAGGACAAATATA+GGG + Chr1:20587544-20587563 MsG0180001401.01.T01:intron 30.0%
TGAGAATAAGTAACATCACT+TGG - Chr1:20585031-20585050 None:intergenic 30.0%
TGATTTCCATTCTCATACTT+CGG + Chr1:20584070-20584089 MsG0180001401.01.T01:intron 30.0%
TTAAAGGAATGATACGCAAT+AGG - Chr1:20584216-20584235 None:intergenic 30.0%
TTCGAGGTTATTCGTTATAT+CGG + Chr1:20584673-20584692 MsG0180001401.01.T01:intron 30.0%
TTGAGAAAGAAGGTTAACAA+AGG - Chr1:20583265-20583284 None:intergenic 30.0%
TTTCATAGTCCATACTGAAT+AGG + Chr1:20583029-20583048 MsG0180001401.01.T01:intron 30.0%
! ATGGTAACGAAATTCAAATG+AGG + Chr1:20583800-20583819 MsG0180001401.01.T01:intron 30.0%
! ATGTTAGTTATGGTTTTCAG+TGG + Chr1:20582693-20582712 MsG0180001401.01.T01:intron 30.0%
! GATAAGGAAATTTTGCAAAG+AGG - Chr1:20587284-20587303 None:intergenic 30.0%
! TAGAATTTTCACTCATAGAG+TGG + Chr1:20584493-20584512 MsG0180001401.01.T01:intron 30.0%
! TATGAAGCACTAAAAAAGCA+AGG - Chr1:20583430-20583449 None:intergenic 30.0%
! TGACTGTGTTGTTAGATAAA+GGG + Chr1:20584999-20585018 MsG0180001401.01.T01:intron 30.0%
! TGCCATTAAACATTGATGAT+TGG + Chr1:20587602-20587621 MsG0180001401.01.T01:intron 30.0%
! TTCCCACATGAAAGTATATT+TGG - Chr1:20584124-20584143 None:intergenic 30.0%
! TTGACTGTGTTGTTAGATAA+AGG + Chr1:20584998-20585017 MsG0180001401.01.T01:intron 30.0%
! TTGCTTTGTTCAAATTCGTA+GGG + Chr1:20585538-20585557 MsG0180001401.01.T01:intron 30.0%
! TTTGCTTTGTTCAAATTCGT+AGG + Chr1:20585537-20585556 MsG0180001401.01.T01:intron 30.0%
! TTTTCGATGTTGTAAGTTTC+TGG + Chr1:20586899-20586918 MsG0180001401.01.T01:intron 30.0%
!! AAAAAGGAATGTTGATTCTG+TGG + Chr1:20584820-20584839 MsG0180001401.01.T01:intron 30.0%
!! GGCTTTTAAAAAAAACATCG+AGG - Chr1:20586666-20586685 None:intergenic 30.0%
!! TAAGAAGCACTAAAAAAGCA+AGG - Chr1:20586808-20586827 None:intergenic 30.0%
!! TGAAAAAACAAAGTGCTGTA+TGG + Chr1:20585796-20585815 MsG0180001401.01.T01:intron 30.0%
!!! TATCATCTGGCTTTGTATTT+TGG + Chr1:20585446-20585465 MsG0180001401.01.T01:CDS 30.0%
!!! TTCTTATTGTCACTTTTAGC+AGG - Chr1:20587508-20587527 None:intergenic 30.0%
AAGGAATGGGAAAAACTTGT+TGG - Chr1:20583411-20583430 None:intergenic 35.0%
ACAAAATTCAAATGAGGCAG+TGG + Chr1:20587184-20587203 MsG0180001401.01.T01:intron 35.0%
ACACTTCTTTATCTTGGTCA+AGG - Chr1:20586687-20586706 None:intergenic 35.0%
ACTTCTCTCTTTCGGATAAA+AGG + Chr1:20582646-20582665 MsG0180001401.01.T01:intron 35.0%
AGCACTAAAAAAGCAAGGAA+TGG - Chr1:20583425-20583444 None:intergenic 35.0%
AGCAGGAAGTTATCATTAGA+TGG - Chr1:20587491-20587510 None:intergenic 35.0%
AGGATTTCAACAAGGAATGA+TGG + Chr1:20583665-20583684 MsG0180001401.01.T01:intron 35.0%
AGTTACACCTAAATCAGACA+AGG + Chr1:20584268-20584287 MsG0180001401.01.T01:intron 35.0%
ATGAATAACTGAGAAGCCAT+AGG - Chr1:20587648-20587667 None:intergenic 35.0%
ATTAGTAACGATTGTGAGTC+CGG + Chr1:20585611-20585630 MsG0180001401.01.T01:intron 35.0%
ATTGGCTGCTACCTATAATT+GGG - Chr1:20586352-20586371 None:intergenic 35.0%
CAATAAGAACTAGGAATGGA+AGG + Chr1:20587519-20587538 MsG0180001401.01.T01:intron 35.0%
CAGGGAAAGATTGTGAAAAA+TGG + Chr1:20583054-20583073 MsG0180001401.01.T01:intron 35.0%
CATCGAAAAGATGTTCTTTG+AGG - Chr1:20586888-20586907 None:intergenic 35.0%
CGAGAAATTTATCGAAAGAG+CGG + Chr1:20587150-20587169 MsG0180001401.01.T01:intron 35.0%
CTAAATAGATAACCTTGTGG+CGG + Chr1:20582886-20582905 MsG0180001401.01.T01:intron 35.0%
CTGAACAACAGGACAAATAT+AGG + Chr1:20587543-20587562 MsG0180001401.01.T01:intron 35.0%
GAGATTGATATGGAGAATGT+TGG + Chr1:20584895-20584914 MsG0180001401.01.T01:intron 35.0%
GATATGGAGAATGTTGGAAA+AGG + Chr1:20584901-20584920 MsG0180001401.01.T01:intron 35.0%
GATTTCAACAAGGAATGATG+GGG + Chr1:20583667-20583686 MsG0180001401.01.T01:intron 35.0%
GCAAAATTAACAGTTGCCTT+CGG + Chr1:20584859-20584878 MsG0180001401.01.T01:intron 35.0%
GCAAAGAAGGGTTGTTAAAA+AGG + Chr1:20584804-20584823 MsG0180001401.01.T01:intron 35.0%
GCACTAAAAAAGCAAGGAAT+GGG - Chr1:20583424-20583443 None:intergenic 35.0%
GGAATTTCAACAAGGAATGA+TGG + Chr1:20587043-20587062 MsG0180001401.01.T01:intron 35.0%
GGATTTCAACAAGGAATGAT+GGG + Chr1:20583666-20583685 MsG0180001401.01.T01:intron 35.0%
GTCTTCACAAATCAATTCAG+AGG + Chr1:20582506-20582525 MsG0180001401.01.T01:CDS 35.0%
GTGACAATAAGAACTAGGAA+TGG + Chr1:20587515-20587534 MsG0180001401.01.T01:intron 35.0%
TAAACCTGGTGCTTATTAAG+TGG + Chr1:20583746-20583765 MsG0180001401.01.T01:intron 35.0%
TCAAATACCTCAAGAAGTCT+AGG - Chr1:20583231-20583250 None:intergenic 35.0%
TCGCTAAATAGATAACCTTG+TGG + Chr1:20586299-20586318 MsG0180001401.01.T01:intron 35.0%
TCTGGTCTATGTTGTAACTT+TGG + Chr1:20583540-20583559 MsG0180001401.01.T01:intron 35.0%
TCTTCAGCTTCACAATGAAT+TGG + Chr1:20585306-20585325 MsG0180001401.01.T01:intron 35.0%
TGGAATTCATTGTCTTCGTT+TGG + Chr1:20582713-20582732 MsG0180001401.01.T01:intron 35.0%
! AAAAGATGTTCTTTGAGGGT+CGG - Chr1:20583506-20583525 None:intergenic 35.0%
! AAAAGATGTTCTTTGAGGGT+TGG - Chr1:20583506-20583525 None:intergenic 35.0%
! ACTTCGTGAAATGGTCATTT+TGG - Chr1:20583207-20583226 None:intergenic 35.0%
! CATTTTTCACAATCTTTCCC+TGG - Chr1:20583056-20583075 None:intergenic 35.0%
! TAAGTTGACTGAGAATGGAT+TGG + Chr1:20583122-20583141 MsG0180001401.01.T01:intron 35.0%
! TCAATTTGAAACTGTTTGCG+AGG + Chr1:20584762-20584781 MsG0180001401.01.T01:intron 35.0%
! TGAAAAACCTTGTCTGGTTT+AGG - Chr1:20587820-20587839 None:intergenic 35.0%
! TGAAGTACCTAGACTTATTG+AGG + Chr1:20586598-20586617 MsG0180001401.01.T01:intron 35.0%
!! GGCATTTAAAAAAAGCATCG+AGG - Chr1:20583289-20583308 None:intergenic 35.0%
!! TAATGAAGCGCTTTTCACTA+AGG - Chr1:20583353-20583372 None:intergenic 35.0%
!!! AATGAAGCCACTTTTCAGTA+AGG - Chr1:20586730-20586749 None:intergenic 35.0%
!!! TCTTCTGCTGTTTTACACAT+AGG - Chr1:20582856-20582875 None:intergenic 35.0%
AATCTGCCAGACCCAATTAT+AGG + Chr1:20582922-20582941 MsG0180001401.01.T01:intron 40.0%
ACAAGACAGAGTCATATCAG+TGG + Chr1:20582602-20582621 MsG0180001401.01.T01:intron 40.0%
ACATCGAGGATTGAGAAAGA+AGG - Chr1:20586652-20586671 None:intergenic 40.0%
ACGAAATTCAAATGAGGCAG+TGG + Chr1:20583806-20583825 MsG0180001401.01.T01:intron 40.0%
ACTATGTTGCAACTCCAATG+AGG - Chr1:20585332-20585351 None:intergenic 40.0%
AGGTTGTCTGTTATTGTGAG+TGG + Chr1:20584399-20584418 MsG0180001401.01.T01:intron 40.0%
AGTCCATACTGAGTAGAACA+AGG + Chr1:20586412-20586431 MsG0180001401.01.T01:intron 40.0%
AGTCTAGGTACTTCGTGAAA+TGG - Chr1:20583216-20583235 None:intergenic 40.0%
AGTTCGACCAACATCATGTT+GGG - Chr1:20584590-20584609 None:intergenic 40.0%
ATAACTGAGAAGCCATAGGT+TGG - Chr1:20587644-20587663 None:intergenic 40.0%
ATCAACTCGCGTTTATCATC+TGG + Chr1:20585433-20585452 MsG0180001401.01.T01:CDS 40.0%
ATCGATGCTCTATGCAAAGA+AGG + Chr1:20584791-20584810 MsG0180001401.01.T01:intron 40.0%
ATCTGCCAGACCCAATTATA+GGG + Chr1:20582923-20582942 MsG0180001401.01.T01:intron 40.0%
ATGCTTTGCTTCATGTTGCT+CGG + Chr1:20584731-20584750 MsG0180001401.01.T01:intron 40.0%
ATTGGCTGCTCCCTATAATT+GGG - Chr1:20582936-20582955 None:intergenic 40.0%
ATTTGCACCTAAACCAGACA+AGG + Chr1:20587810-20587829 MsG0180001401.01.T01:intron 40.0%
CAGAGAGAGTGAATACTTCA+AGG - Chr1:20584160-20584179 None:intergenic 40.0%
CAGCAAACCTTACTGAAAAG+TGG + Chr1:20586720-20586739 MsG0180001401.01.T01:intron 40.0%
CCAAGATAGAGTCATATCAG+TGG + Chr1:20586018-20586037 MsG0180001401.01.T01:CDS 40.0%
CCACTGATATGACTCTATCT+TGG - Chr1:20586021-20586040 None:intergenic 40.0%
CCTTCTTCCTCATATGTCAA+GGG + Chr1:20586554-20586573 MsG0180001401.01.T01:intron 40.0%
CGAGAAATTGATCGAAAGAG+CGG + Chr1:20583772-20583791 MsG0180001401.01.T01:intron 40.0%
CTGTTATTGAGTGGAGTTTG+TGG + Chr1:20587952-20587971 MsG0180001401.01.T01:CDS 40.0%
GAAATTTATCGAAAGAGCGG+TGG + Chr1:20587153-20587172 MsG0180001401.01.T01:intron 40.0%
GAAGAGGAAAAAGACGATGA+AGG + Chr1:20584923-20584942 MsG0180001401.01.T01:intron 40.0%
GAAGGTTGTCTGTTATTGAG+TGG + Chr1:20587943-20587962 MsG0180001401.01.T01:intron 40.0%
GAGAATGTTGGAAAAGGAAG+AGG + Chr1:20584907-20584926 MsG0180001401.01.T01:intron 40.0%
GATTCTGTGGATCAGATTGT+TGG + Chr1:20584833-20584852 MsG0180001401.01.T01:intron 40.0%
GTCCATACTGAGTAGAACAA+GGG + Chr1:20586413-20586432 MsG0180001401.01.T01:intron 40.0%
GTTATTGTGAGTGGAGTTTG+TGG + Chr1:20584408-20584427 MsG0180001401.01.T01:intron 40.0%
TAACTGAGAAGCCATAGGTT+GGG - Chr1:20587643-20587662 None:intergenic 40.0%
TAATGCAAGAGGCTTAGTCA+AGG - Chr1:20584618-20584637 None:intergenic 40.0%
TCCTTCTTCCTCATATGTCA+AGG + Chr1:20586553-20586572 MsG0180001401.01.T01:intron 40.0%
TCGATGCTCTATGCAAAGAA+GGG + Chr1:20584792-20584811 MsG0180001401.01.T01:intron 40.0%
TGGAATTCATTGTCTCCGTT+TGG + Chr1:20586129-20586148 MsG0180001401.01.T01:intron 40.0%
TGGTGAGGTAAATCACACAA+GGG - Chr1:20583463-20583482 None:intergenic 40.0%
TTAGTAACGATTGTGAGTCC+GGG + Chr1:20585612-20585631 MsG0180001401.01.T01:intron 40.0%
TTATTGAGTGGAGTTTGTGG+TGG + Chr1:20587955-20587974 MsG0180001401.01.T01:CDS 40.0%
TTCCCTTGTTCTACTCAGTA+TGG - Chr1:20586418-20586437 None:intergenic 40.0%
! AGATGATAAACGCGAGTTGA+TGG - Chr1:20585434-20585453 None:intergenic 40.0%
! ATATGAGGAAGAAGGAGCAT+TGG - Chr1:20586549-20586568 None:intergenic 40.0%
! CATTGGCTGCTACCTATAAT+TGG - Chr1:20586353-20586372 None:intergenic 40.0%
! CTTGAAGTATTCACTCTCTC+TGG + Chr1:20584158-20584177 MsG0180001401.01.T01:intron 40.0%
! GTGAGTAAGTTGACTGAGAA+TGG + Chr1:20583117-20583136 MsG0180001401.01.T01:intron 40.0%
! GTTGGCTCCTTAAGATTAAG+AGG - Chr1:20583393-20583412 None:intergenic 40.0%
! TCACAATGAATTGGCCTCAT+TGG + Chr1:20585315-20585334 MsG0180001401.01.T01:intron 40.0%
! TCAGTTTTTCTCCCAACCTA+TGG + Chr1:20587629-20587648 MsG0180001401.01.T01:intron 40.0%
! TGATCATAAGAGTGCAACCA+AGG - Chr1:20585688-20585707 None:intergenic 40.0%
!! GATGATAAACGCGAGTTGAT+GGG - Chr1:20585433-20585452 None:intergenic 40.0%
!! TATAGATGATGGAATCTGCC+TGG - Chr1:20583483-20583502 None:intergenic 40.0%
!! TTGAGGGTTGGTATAGATGA+TGG - Chr1:20586871-20586890 None:intergenic 40.0%
!!! ATTCAGTGTTTGTGCCGTTT+TGG + Chr1:20585160-20585179 MsG0180001401.01.T01:intron 40.0%
!!! CATCTGGCTTTGTATTTTGG+AGG + Chr1:20585449-20585468 MsG0180001401.01.T01:CDS 40.0%
!!! TAACATGCGCTGCTTTTGAA+AGG - Chr1:20585362-20585381 None:intergenic 40.0%
ACTTCCTTCCATCGAACTAG+AGG - Chr1:20583844-20583863 None:intergenic 45.0%
AGGAGACTGATGCTTCTCAA+CGG - Chr1:20587264-20587283 None:intergenic 45.0%
AGTCCATACTGAATAGGACC+AGG + Chr1:20583035-20583054 MsG0180001401.01.T01:intron 45.0%
ATCTTAGTCGTACACGCCAA+TGG + Chr1:20583153-20583172 MsG0180001401.01.T01:intron 45.0%
ATTGTGAGTGGAGTTTGTGG+TGG + Chr1:20584411-20584430 MsG0180001401.01.T01:intron 45.0%
CAACATCATGTTGGGAGAGT+TGG - Chr1:20584582-20584601 None:intergenic 45.0%
CAACTCTCCCAACATGATGT+TGG + Chr1:20584580-20584599 MsG0180001401.01.T01:intron 45.0%
CAATGGTCCTTCTTCCTCAT+AGG + Chr1:20583170-20583189 MsG0180001401.01.T01:intron 45.0%
CCAGGTTCTTGTGTTTCCTT+AGG - Chr1:20586475-20586494 None:intergenic 45.0%
CCCTTGACATATGAGGAAGA+AGG - Chr1:20586557-20586576 None:intergenic 45.0%
CCTAAGGAAACACAAGAACC+TGG + Chr1:20586472-20586491 MsG0180001401.01.T01:intron 45.0%
CCTTCTTCCTCATAGGTCAA+GGG + Chr1:20583177-20583196 MsG0180001401.01.T01:intron 45.0%
CTATGAGGAAGAAGGACCAT+TGG - Chr1:20583172-20583191 None:intergenic 45.0%
CTGCTACCTATAATTGGGTC+TGG - Chr1:20586347-20586366 None:intergenic 45.0%
CTGGTGAGGTAAATCACACA+AGG - Chr1:20583464-20583483 None:intergenic 45.0%
CTTCCTTCCATCGAACTAGA+GGG - Chr1:20583843-20583862 None:intergenic 45.0%
CTTCCTTCCATGGAACTAGA+GGG - Chr1:20587221-20587240 None:intergenic 45.0%
CTTGTGTGATTTACCTCACC+AGG + Chr1:20583462-20583481 MsG0180001401.01.T01:intron 45.0%
GAAATTGATCGAAAGAGCGG+TGG + Chr1:20583775-20583794 MsG0180001401.01.T01:intron 45.0%
GAATGGAAGGACTGAACAAC+AGG + Chr1:20587532-20587551 MsG0180001401.01.T01:intron 45.0%
GACTATGAAAGGTCGATTGC+TGG + Chr1:20585213-20585232 MsG0180001401.01.T01:CDS 45.0%
GAGTTCGACCAACATCATGT+TGG - Chr1:20584591-20584610 None:intergenic 45.0%
GATGGAAGGAAGTGAATCCT+CGG + Chr1:20583851-20583870 MsG0180001401.01.T01:intron 45.0%
GCAACCACTTAATAAGCACC+AGG - Chr1:20583753-20583772 None:intergenic 45.0%
GCAGAAGGGAAATGACGAAA+GGG + Chr1:20585282-20585301 MsG0180001401.01.T01:intron 45.0%
GCATCGAGGATTGAGAAAGA+AGG - Chr1:20583275-20583294 None:intergenic 45.0%
GGGAAACCGAAGTATGAGAA+TGG - Chr1:20584079-20584098 None:intergenic 45.0%
GTCCATACTGAATAGGACCA+GGG + Chr1:20583036-20583055 MsG0180001401.01.T01:intron 45.0%
GTGAGAAATGTCCAGGTTCA+GGG + Chr1:20585067-20585086 MsG0180001401.01.T01:intron 45.0%
TATCGAAAGAGCGGTGGTAA+TGG + Chr1:20587159-20587178 MsG0180001401.01.T01:intron 45.0%
TATGAGACGTTGTTGATCGC+TGG + Chr1:20585253-20585272 MsG0180001401.01.T01:intron 45.0%
TATTCACTCTCTCTGGATCG+TGG + Chr1:20584165-20584184 MsG0180001401.01.T01:intron 45.0%
TCCTTCTTCCTCATAGGTCA+AGG + Chr1:20583176-20583195 MsG0180001401.01.T01:intron 45.0%
TCTCAGTCAACTTACTCACC+AGG - Chr1:20583116-20583135 None:intergenic 45.0%
TCTGAGTAACGAGAGCCTAA+GGG + Chr1:20583080-20583099 MsG0180001401.01.T01:intron 45.0%
TCTTTCCCTGTTGAGTGCAT+TGG - Chr1:20582954-20582973 None:intergenic 45.0%
TGCAGAAGGGAAATGACGAA+AGG + Chr1:20585281-20585300 MsG0180001401.01.T01:intron 45.0%
TGGATAGCCCTTGACATATG+AGG - Chr1:20586564-20586583 None:intergenic 45.0%
TGGCTATGTGAGAAATGTCC+AGG + Chr1:20585060-20585079 MsG0180001401.01.T01:CDS 45.0%
TGTGAGAAATGTCCAGGTTC+AGG + Chr1:20585066-20585085 MsG0180001401.01.T01:intron 45.0%
TTCCATCGAACTAGAGGGAT+TGG - Chr1:20583838-20583857 None:intergenic 45.0%
TTCCATGGAACTAGAGGGAT+TGG - Chr1:20587216-20587235 None:intergenic 45.0%
TTCCCTGGTCCTATTCAGTA+TGG - Chr1:20583041-20583060 None:intergenic 45.0%
TTGAGGGTCGGTATAGATGA+TGG - Chr1:20583494-20583513 None:intergenic 45.0%
TTTATGCTTCCCAACGGTTC+AGG - Chr1:20583880-20583899 None:intergenic 45.0%
TTTCGTCATTTCCCTTCTGC+AGG - Chr1:20585282-20585301 None:intergenic 45.0%
! AATCCCTCTAGTTCCATGGA+AGG + Chr1:20587215-20587234 MsG0180001401.01.T01:intron 45.0%
! AATCCCTCTAGTTCGATGGA+AGG + Chr1:20583837-20583856 MsG0180001401.01.T01:intron 45.0%
! ATATGTTGACGCTGCTTTCG+AGG + Chr1:20584657-20584676 MsG0180001401.01.T01:intron 45.0%
! ATCCAATCCCTCTAGTTCCA+TGG + Chr1:20587211-20587230 MsG0180001401.01.T01:intron 45.0%
! ATCCAATCCCTCTAGTTCGA+TGG + Chr1:20583833-20583852 MsG0180001401.01.T01:intron 45.0%
! CATTGGCTGCTCCCTATAAT+TGG - Chr1:20582937-20582956 None:intergenic 45.0%
! CGAAGTACCTAGACTTCTTG+AGG + Chr1:20583221-20583240 MsG0180001401.01.T01:intron 45.0%
!! AGAGGATTAGCTTCCTTCCA+TGG - Chr1:20587231-20587250 None:intergenic 45.0%
!!! GTGTTTGTGCCGTTTTGGAA+AGG + Chr1:20585165-20585184 MsG0180001401.01.T01:intron 45.0%
!!! TTTTTATTGATTATTTATTT+CGG - Chr1:20584100-20584119 None:intergenic 5.0%
AGCAGCCAATGCACTCAACA+GGG + Chr1:20582946-20582965 MsG0180001401.01.T01:intron 50.0%
ATTGGGTCTGGCAGATTCCT+TGG - Chr1:20582919-20582938 None:intergenic 50.0%
CCAGGTTCTTGTGTTCCCTT+AGG - Chr1:20583098-20583117 None:intergenic 50.0%
CCCTTGACCTATGAGGAAGA+AGG - Chr1:20583180-20583199 None:intergenic 50.0%
CCTAAGGGAACACAAGAACC+TGG + Chr1:20583095-20583114 MsG0180001401.01.T01:intron 50.0%
CTCTGAGTAACGAGAGCCTA+AGG + Chr1:20583079-20583098 MsG0180001401.01.T01:intron 50.0%
CTGCTCCCTATAATTGGGTC+TGG - Chr1:20582931-20582950 None:intergenic 50.0%
CTTTGCTTCATGTTGCTCGG+AGG + Chr1:20584734-20584753 MsG0180001401.01.T01:intron 50.0%
GCTTCCTTCCATGGAACTAG+AGG - Chr1:20587222-20587241 None:intergenic 50.0%
TCTTCAGAACTCCCTGAACC+TGG - Chr1:20585081-20585100 None:intergenic 50.0%
! ATGATGGAATCTGCCTGGTG+AGG - Chr1:20583478-20583497 None:intergenic 50.0%
ATCGCTGGTCACCTGCAGAA+GGG + Chr1:20585268-20585287 MsG0180001401.01.T01:intron 55.0%
CTTGGACTCTTTCCGCCACA+AGG - Chr1:20582901-20582920 None:intergenic 55.0%
GAGCAGCCAATGCACTCAAC+AGG + Chr1:20582945-20582964 MsG0180001401.01.T01:intron 55.0%
GATCGAAAGAGCGGTGGTGA+TGG + Chr1:20583781-20583800 MsG0180001401.01.T01:intron 55.0%
! CTTGTGGCGGAAAGAGTCCA+AGG + Chr1:20582899-20582918 MsG0180001401.01.T01:intron 55.0%
! TGGCTTGCCCTTGACCTATG+AGG - Chr1:20583187-20583206 None:intergenic 55.0%
CCTCGGACACCTGAACCGTT+GGG + Chr1:20583868-20583887 MsG0180001401.01.T01:intron 60.0%
CTCAACGGCTCAGCTGTCAG+AGG - Chr1:20587249-20587268 None:intergenic 60.0%
GATCGCTGGTCACCTGCAGA+AGG + Chr1:20585267-20585286 MsG0180001401.01.T01:intron 60.0%
TCCTCGGACACCTGAACCGT+TGG + Chr1:20583867-20583886 MsG0180001401.01.T01:intron 60.0%
CCCAACGGTTCAGGTGTCCG+AGG - Chr1:20583871-20583890 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 20582462 20588022 20582462 ID=MsG0180001401.01;Name=MsG0180001401.01
Chr1 mRNA 20582462 20588022 20582462 ID=MsG0180001401.01.T01;Parent=MsG0180001401.01;Name=MsG0180001401.01.T01;_AED=0.66;_eAED=0.69;_QI=0|0|0|0.83|0|0|6|0|245
Chr1 exon 20582462 20582591 20582462 ID=MsG0180001401.01.T01:exon:14813;Parent=MsG0180001401.01.T01
Chr1 exon 20585014 20585081 20585014 ID=MsG0180001401.01.T01:exon:14814;Parent=MsG0180001401.01.T01
Chr1 exon 20585187 20585243 20585187 ID=MsG0180001401.01.T01:exon:14815;Parent=MsG0180001401.01.T01
Chr1 exon 20585351 20585554 20585351 ID=MsG0180001401.01.T01:exon:14816;Parent=MsG0180001401.01.T01
Chr1 exon 20585881 20586083 20585881 ID=MsG0180001401.01.T01:exon:14817;Parent=MsG0180001401.01.T01
Chr1 exon 20587947 20588022 20587947 ID=MsG0180001401.01.T01:exon:14818;Parent=MsG0180001401.01.T01
Chr1 CDS 20582462 20582591 20582462 ID=MsG0180001401.01.T01:cds;Parent=MsG0180001401.01.T01
Chr1 CDS 20585014 20585081 20585014 ID=MsG0180001401.01.T01:cds;Parent=MsG0180001401.01.T01
Chr1 CDS 20585187 20585243 20585187 ID=MsG0180001401.01.T01:cds;Parent=MsG0180001401.01.T01
Chr1 CDS 20585351 20585554 20585351 ID=MsG0180001401.01.T01:cds;Parent=MsG0180001401.01.T01
Chr1 CDS 20585881 20586083 20585881 ID=MsG0180001401.01.T01:cds;Parent=MsG0180001401.01.T01
Chr1 CDS 20587947 20588022 20587947 ID=MsG0180001401.01.T01:cds;Parent=MsG0180001401.01.T01
Gene Sequence

>MsG0180001401.01.T01

ATGGATAATTGTTTTGAAGATTATGGAGAAATTGTTAAACATTTGTCTTCACAAATCAATTCAGAGGTTGAAGACATACTGAGTTTGCAACAGCAGAAAATGTGTAGAAAGCAAGCTTTTATAGCTGACTATAAAGGGTTTTTGCCAAGTGATGTTACTTATTCTCATTTGATATATGGCTATGTGAGAAATGTCCAGGTTGAAAATACTGACAAGTATTTAAGGACTATGAAAGGTCGATTGCTGGTTCCAAATTTAGTAAGCCTTTCAAAAGCAGCGCATGTTATTTACGTGAAATTGTTGTTACTAAGTTTTAAGCAAGTTCGGATCATAACCCATCAACTCGCGTTTATCATCTGGCTTTGTATTTTGGAGGTATGTTTCAGTTTTTCTATTTATAGAGCTGTCATTATTTTTATGTTTGAATGTAGATTTAACATCTTTGCTTTGTTCAAATTCGATAATTGTTTTGAAGATTATGGAGAAATTGTTAAACATTTGTCTTCACAAATCAATTCAGAGGTTGAAGACATACTGAGTTTGCAACAGCAGAAAATGTGTAGAAAGCAAGCTTTTATAGCTGACTGTAAGAACTTCCAAGATAGAGTCATATCAGTGGAAGCAATAAGCTTACATATATACTTCTCTCTTTCGGATAAAAGGTTGTCTGTTATTGAGTGGAGTTTGTGGTGGAAAGTGAAAGTGATTATGCTTTACAGTTCAAGTGGAACCCATTAA
Protein sequence

>MsG0180001401.01.T01

MDNCFEDYGEIVKHLSSQINSEVEDILSLQQQKMCRKQAFIADYKGFLPSDVTYSHLIYGYVRNVQVENTDKYLRTMKGRLLVPNLVSLSKAAHVIYVKLLLLSFKQVRIITHQLAFIIWLCILEVCFSFSIYRAVIIFMFECRFNIFALFKFDNCFEDYGEIVKHLSSQINSEVEDILSLQQQKMCRKQAFIADCKNFQDRVISVEAISLHIYFSLSDKRLSVIEWSLWWKVKVIMLYSSSGTH*