AlfalfaGEDB | MsG0380014977.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014977.01

KEGG: -
GO: GO:0003677 GO:0046983
PFAM: PF00319

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014977.01.T01	XP_024634671.1	88.722	133	15	0	1	133	6	138	1.30E-81	246

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014977.01.T01	Q9FJK3	44	125	67	2	4	127	32	154	1.36E-28	109

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014977.01.T01	A0A072UY35	88.722	133	15	0	1	133	29	161	7.78e-82	246

TFs/TRs:

Gene ID	Type	Classification
MsG0380014977.01.T01	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047925.01	MsG0380014977.01	0.827499	1.512300e-54	1.462582e-51
MsG0080048431.01	MsG0380014977.01	0.827157	1.826518e-54	1.748920e-51
MsG0180000748.01	MsG0380014977.01	0.803128	4.040328e-49	2.014224e-46
MsG0180001401.01	MsG0380014977.01	0.808811	2.578583e-50	1.488758e-47
MsG0180001410.01	MsG0380014977.01	0.803294	3.734269e-49	1.869564e-46
MsG0180002195.01	MsG0380014977.01	0.813860	2.066841e-51	1.365239e-48
MsG0180002233.01	MsG0380014977.01	0.802748	4.841134e-49	2.390308e-46
MsG0180002234.01	MsG0380014977.01	0.830673	2.571875e-55	2.728564e-52
MsG0180002518.01	MsG0380014977.01	0.845034	5.221791e-59	8.590741e-56
MsG0180002667.01	MsG0380014977.01	0.814646	1.385884e-51	9.351627e-49
MsG0180004847.01	MsG0380014977.01	0.814335	1.623484e-51	1.086322e-48
MsG0280006325.01	MsG0380014977.01	0.835954	1.241875e-56	1.542254e-53
MsG0380011574.01	MsG0380014977.01	0.820671	6.064811e-53	4.829471e-50
MsG0380011786.01	MsG0380014977.01	0.804915	1.717857e-49	8.967782e-47
MsG0380013489.01	MsG0380014977.01	0.819308	1.243649e-52	9.534778e-50
MsG0380013596.01	MsG0380014977.01	0.800766	1.235325e-48	5.799922e-46
MsG0380013883.01	MsG0380014977.01	0.856147	3.915641e-62	9.255419e-59
MsG0380013896.01	MsG0380014977.01	0.842022	3.332616e-58	4.987000e-55
MsG0380014422.01	MsG0380014977.01	0.802927	4.446242e-49	2.205294e-46
MsG0380014472.01	MsG0380014977.01	0.822970	1.781679e-53	1.513960e-50
MsG0380014976.01	MsG0380014977.01	0.831763	1.387996e-55	1.521032e-52
MsG0380014977.01	MsG0380016274.01	0.826262	2.987800e-54	2.787965e-51
MsG0380014977.01	MsG0380017312.01	0.842989	1.845729e-58	2.846869e-55
MsG0380014977.01	MsG0380017820.01	0.858074	1.057156e-62	2.665346e-59
MsG0380014977.01	MsG0380017821.01	0.855971	4.409255e-62	1.036133e-58
MsG0380014977.01	MsG0380017822.01	0.823542	1.309996e-53	1.131209e-50
MsG0380014977.01	MsG0480018664.01	0.854806	9.632514e-62	2.177843e-58
MsG0380014977.01	MsG0480019482.01	0.816376	5.710600e-52	4.039585e-49
MsG0380014977.01	MsG0480021587.01	0.813699	2.243065e-51	1.475222e-48
MsG0380014977.01	MsG0580024621.01	0.806398	8.388671e-50	4.548332e-47
MsG0380014977.01	MsG0580026197.01	0.812682	3.749137e-51	2.399150e-48
MsG0380014977.01	MsG0580026222.01	0.908606	1.422348e-81	2.658457e-77
MsG0380014977.01	MsG0580026275.01	0.837679	4.507283e-57	5.899292e-54
MsG0380014977.01	MsG0580026276.01	0.849620	2.876813e-60	5.486307e-57
MsG0380014977.01	MsG0580026303.01	0.806576	7.690738e-50	4.189034e-47
MsG0380014977.01	MsG0580026434.01	0.841659	4.156194e-58	6.148716e-55
MsG0380014977.01	MsG0580026631.01	0.839763	1.304124e-57	1.818926e-54
MsG0380014977.01	MsG0580027332.01	0.876834	1.018303e-68	5.009952e-65
MsG0380014977.01	MsG0580027687.01	0.803420	3.515857e-49	1.765993e-46
MsG0380014977.01	MsG0580027688.01	0.823837	1.117325e-53	9.730041e-51
MsG0380014977.01	MsG0580028169.01	0.801748	7.776441e-49	3.742870e-46
MsG0380014977.01	MsG0580028170.01	0.809320	2.005312e-50	1.173707e-47
MsG0380014977.01	MsG0580029381.01	0.913313	7.114242e-84	1.672184e-79
MsG0380014977.01	MsG0580029899.01	0.842031	3.313583e-58	4.959926e-55
MsG0380014977.01	MsG0580030168.01	0.804323	2.282262e-49	1.173273e-46
MsG0380014977.01	MsG0680031380.01	0.876670	1.160708e-68	5.676475e-65
MsG0380014977.01	MsG0680031896.01	0.862491	4.881935e-64	1.432482e-60
MsG0380014977.01	MsG0680031989.01	0.807144	5.833658e-50	3.224477e-47
MsG0380014977.01	MsG0680032844.01	0.813231	2.842716e-51	1.845840e-48
MsG0380014977.01	MsG0680033038.01	0.812308	4.524845e-51	2.867008e-48
MsG0380014977.01	MsG0680033466.01	0.835633	1.497780e-56	1.842193e-53
MsG0380014977.01	MsG0680034264.01	0.803588	3.244669e-49	1.636907e-46
MsG0380014977.01	MsG0680034538.01	0.817408	3.349966e-52	2.437640e-49
MsG0380014977.01	MsG0680035115.01	0.807567	4.745810e-50	2.652139e-47
MsG0380014977.01	MsG0680035896.01	0.875399	3.178329e-68	1.481637e-64
MsG0380014977.01	MsG0780036134.01	0.818184	2.238072e-52	1.663581e-49
MsG0380014977.01	MsG0780036498.01	0.814737	1.322541e-51	8.946126e-49
MsG0380014977.01	MsG0780036614.01	0.803995	2.670972e-49	1.361644e-46
MsG0380014977.01	MsG0780037606.01	0.841041	6.042618e-58	8.769176e-55
MsG0380014977.01	MsG0780039282.01	0.909038	8.855786e-82	1.690603e-77
MsG0380014977.01	MsG0780039880.01	0.840078	1.079503e-57	1.520806e-54
MsG0380014977.01	MsG0780040767.01	0.808470	3.048270e-50	1.744461e-47
MsG0380014977.01	MsG0880041916.01	0.832075	1.162806e-55	1.285823e-52
MsG0380014977.01	MsG0880044644.01	0.884303	2.151232e-71	1.412301e-67
MsG0380014977.01	MsG0880044788.01	0.878178	3.465362e-69	1.791642e-65
MsG0380014977.01	MsG0880045045.01	0.817509	3.178909e-52	2.319619e-49
MsG0280006551.01	MsG0380014977.01	0.853361	2.514053e-61	5.418132e-58
MsG0280008759.01	MsG0380014977.01	0.860652	1.779313e-63	4.899889e-60
MsG0280009248.01	MsG0380014977.01	0.837769	4.273805e-57	5.609280e-54
MsG0280009306.01	MsG0380014977.01	0.859565	3.789091e-63	1.004989e-59
MsG0280009371.01	MsG0380014977.01	0.862824	3.854133e-64	1.144243e-60

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014977.01.T01	MTR_3g467080	88.722	133	15	0	1	133	29	161	1.97e-85	246
MsG0380014977.01.T01	MTR_4g063790	79.699	133	27	0	1	133	29	161	8.78e-75	220
MsG0380014977.01.T01	MTR_3g465410	78.947	133	28	0	1	133	1	133	1.84e-73	216
MsG0380014977.01.T01	MTR_3g466980	50.000	126	62	1	1	126	29	153	2.34e-40	134
MsG0380014977.01.T01	MTR_3g065100	44.882	127	69	1	4	130	32	157	9.08e-35	120
MsG0380014977.01.T01	MTR_3g031100	46.512	129	69	0	1	129	29	157	5.57e-34	119
MsG0380014977.01.T01	MTR_3g031240	43.939	132	74	0	1	132	29	160	2.63e-31	110
MsG0380014977.01.T01	MTR_2g016210	40.909	132	78	0	1	132	29	160	3.13e-31	111
MsG0380014977.01.T01	MTR_2g035580	46.825	126	66	1	2	127	30	154	4.88e-30	106
MsG0380014977.01.T01	MTR_5g075380	38.889	126	76	1	1	126	29	153	1.07e-28	103
MsG0380014977.01.T01	MTR_4g032290	40.000	130	78	0	4	133	32	161	1.12e-28	104
MsG0380014977.01.T01	MTR_4g032260	36.508	126	80	0	6	131	34	159	3.76e-27	99.0
MsG0380014977.01.T01	MTR_4g032620	37.302	126	79	0	1	126	29	154	4.95e-27	100
MsG0380014977.01.T01	MTR_2g035610	47.253	91	48	0	4	94	32	122	5.00e-27	97.4
MsG0380014977.01.T01	MTR_4g031910	38.462	130	80	0	4	133	32	161	3.99e-26	98.2
MsG0380014977.01.T01	MTR_8g036130	39.231	130	77	1	1	128	29	158	1.99e-25	97.1
MsG0380014977.01.T01	MTR_3g466830	38.400	125	76	1	1	125	28	151	3.21e-23	89.0
MsG0380014977.01.T01	MTR_3g466900	33.333	126	83	1	4	129	2	126	6.56e-20	79.7
MsG0380014977.01.T01	MTR_1g077320	33.333	129	82	3	4	131	32	157	2.50e-19	79.0
MsG0380014977.01.T01	MTR_1g077300	31.538	130	85	2	4	133	32	157	2.67e-18	76.3
MsG0380014977.01.T01	MTR_3g466890	34.400	125	81	1	2	126	29	152	3.26e-18	76.3
MsG0380014977.01.T01	MTR_3g466930	34.146	123	80	1	4	126	2	123	6.77e-18	74.7
MsG0380014977.01.T01	MTR_5g047580	33.333	129	84	2	1	129	29	155	7.08e-18	75.1
MsG0380014977.01.T01	MTR_4g028720	30.769	130	88	2	4	133	32	159	2.31e-17	73.9
MsG0380014977.01.T01	MTR_1g090783	31.783	129	86	2	1	129	28	154	2.79e-17	73.6
MsG0380014977.01.T01	MTR_7g011950	30.469	128	87	2	4	131	32	157	3.08e-17	73.6
MsG0380014977.01.T01	MTR_1g077390	34.328	134	80	4	1	131	29	157	2.22e-16	71.2
MsG0380014977.01.T01	MTR_1g084950	30.709	127	84	3	4	129	32	155	8.89e-16	69.7
MsG0380014977.01.T01	MTR_7g055800	40.476	84	35	1	3	86	18	86	1.41e-15	67.4
MsG0380014977.01.T01	MTR_1g090697	29.921	127	85	3	1	126	29	152	1.91e-13	63.5
MsG0380014977.01.T01	MTR_7g055940	39.474	76	31	1	3	78	18	78	4.39e-13	60.8
MsG0380014977.01.T01	MTR_1g090710	29.771	131	87	3	1	129	29	156	6.05e-13	62.4
MsG0380014977.01.T01	MTR_4g084780	53.704	54	25	0	46	99	1	54	3.66e-12	60.1
MsG0380014977.01.T01	MTR_4g028800	51.111	45	22	0	1	45	27	71	6.39e-12	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014977.01.T01	AT5G48670	44.000	125	67	2	4	127	32	154	1.38e-29	109
MsG0380014977.01.T01	AT5G27810	51.685	89	43	0	1	89	1	89	1.11e-28	101
MsG0380014977.01.T01	AT1G65300	38.889	126	76	1	2	127	29	153	1.04e-22	90.5
MsG0380014977.01.T01	AT1G65330	38.525	122	74	1	1	122	28	148	5.67e-20	83.2
MsG0380014977.01.T01	AT3G05860	37.903	124	72	2	1	122	29	149	5.17e-19	79.3
MsG0380014977.01.T01	AT5G26650	34.884	129	83	1	1	129	27	154	6.42e-19	81.3
MsG0380014977.01.T01	AT3G05860	52.113	71	34	0	1	71	29	99	1.07e-18	79.3
MsG0380014977.01.T01	AT3G05860	52.113	71	34	0	1	71	29	99	1.48e-18	79.0
MsG0380014977.01.T01	AT5G27960	32.558	129	86	1	1	129	27	154	1.01e-16	75.1
MsG0380014977.01.T01	AT1G31640	34.815	135	74	4	2	130	29	155	4.35e-16	73.9
MsG0380014977.01.T01	AT5G26630	30.579	121	83	1	6	126	34	153	1.17e-15	70.9
MsG0380014977.01.T01	AT1G22590	31.061	132	74	2	1	129	29	146	3.23e-14	65.9
MsG0380014977.01.T01	AT2G28700	31.148	122	83	1	1	122	29	149	1.90e-13	65.9
MsG0380014977.01.T01	AT1G31630	33.333	132	70	5	3	126	30	151	2.84e-13	65.5

Find 24 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGGTCATTTCCTGCTTTC+TGG	0.066665	3:+63509262	None:intergenic
ATTCATACTACAGAATTTAA+TGG	0.196701	3:-63509224	MsG0380014977.01.T01:CDS
CAAGTTTGGCCATCAGATTC+AGG	0.225208	3:-63509419	MsG0380014977.01.T01:CDS
GTTCAGAAGTTTGCCTGGAC+TGG	0.390262	3:-63509375	MsG0380014977.01.T01:CDS
ACAATCCTCAAGCACAAGTT+TGG	0.390704	3:-63509433	MsG0380014977.01.T01:CDS
GATGAAATTAGCACTCTTTG+CGG	0.422591	3:-63509488	MsG0380014977.01.T01:CDS
AATGAAATGATGAATGATAT+TGG	0.442815	3:+63509244	None:intergenic
TATTCTTGAGGCAAAGCATT+GGG	0.452810	3:-63509322	MsG0380014977.01.T01:CDS
TCCAGGTTCAGAAGTTTGCC+TGG	0.467504	3:-63509380	MsG0380014977.01.T01:CDS
AAAGAAGGAAACCAGAAAGC+AGG	0.481583	3:-63509273	MsG0380014977.01.T01:CDS
AAGTTTGGCCATCAGATTCA+GGG	0.497829	3:-63509418	MsG0380014977.01.T01:CDS
GGGTTAGAAGTGTGATATCC+AGG	0.506627	3:-63509397	MsG0380014977.01.T01:CDS
CAAATTCTCAGCAATAAAAG+AGG	0.520681	3:+63509166	None:intergenic
TCCAGGCAAACTTCTGAACC+TGG	0.543284	3:+63509379	None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG	0.555152	3:-63509323	MsG0380014977.01.T01:CDS
GCAGTTGATGAAACAAAAGA+AGG	0.561272	3:-63509288	MsG0380014977.01.T01:CDS
TTGCTCGTAAATTATTGCAC+CGG	0.562136	3:+63509457	None:intergenic
GATGGCCAAACTTGTGCTTG+AGG	0.578295	3:+63509428	None:intergenic
ACTCTTTGCGGAGTAGAAGC+CGG	0.579730	3:-63509476	MsG0380014977.01.T01:CDS
GAGGTCGATCAAAAGATGAA+AGG	0.605953	3:-63509140	MsG0380014977.01.T01:CDS
CTTCTAACCCCTGAATCTGA+TGG	0.608216	3:+63509410	None:intergenic
AGTTTGGCCATCAGATTCAG+GGG	0.628974	3:-63509417	MsG0380014977.01.T01:CDS
TATTGCTGAGAATTTGAGGG+AGG	0.639350	3:-63509159	MsG0380014977.01.T01:CDS
TGGATCAAGAGGTATTCTTG+AGG	0.709854	3:-63509334	MsG0380014977.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGAAATGATGAATGATAT+TGG	+	Chr3:63509380-63509399	None:intergenic	20.0%
!!	ATTCATACTACAGAATTTAA+TGG	-	Chr3:63509397-63509416	MsG0380014977.01.T01:CDS	20.0%
!!	TTTTATTGCTGAGAATTTGA+GGG	-	Chr3:63509459-63509478	MsG0380014977.01.T01:CDS	25.0%
	ACTGGAACAAAGTAAAAAGA+TGG	-	Chr3:63509264-63509283	MsG0380014977.01.T01:CDS	30.0%
	CAAATTCTCAGCAATAAAAG+AGG	+	Chr3:63509458-63509477	None:intergenic	30.0%
!	CTTTTATTGCTGAGAATTTG+AGG	-	Chr3:63509458-63509477	MsG0380014977.01.T01:CDS	30.0%
	GATGAAATTAGCACTCTTTG+CGG	-	Chr3:63509133-63509152	MsG0380014977.01.T01:CDS	35.0%
	GGAACAAAGTAAAAAGATGG+TGG	-	Chr3:63509267-63509286	MsG0380014977.01.T01:CDS	35.0%
	TTGCTCGTAAATTATTGCAC+CGG	+	Chr3:63509167-63509186	None:intergenic	35.0%
!	TATTCTTGAGGCAAAGCATT+GGG	-	Chr3:63509299-63509318	MsG0380014977.01.T01:CDS	35.0%
!	TTTTTACTTTGTTCCAGTCC+AGG	+	Chr3:63509262-63509281	None:intergenic	35.0%
!!	GCAGTTGATGAAACAAAAGA+AGG	-	Chr3:63509333-63509352	MsG0380014977.01.T01:CDS	35.0%
!!	TAAAAAGATGGTGGATCAAG+AGG	-	Chr3:63509276-63509295	MsG0380014977.01.T01:CDS	35.0%
	AAAGAAGGAAACCAGAAAGC+AGG	-	Chr3:63509348-63509367	MsG0380014977.01.T01:CDS	40.0%
	AAGTTTGGCCATCAGATTCA+GGG	-	Chr3:63509203-63509222	MsG0380014977.01.T01:CDS	40.0%
	ACAATCCTCAAGCACAAGTT+TGG	-	Chr3:63509188-63509207	MsG0380014977.01.T01:CDS	40.0%
	ATTGGTCATTTCCTGCTTTC+TGG	+	Chr3:63509362-63509381	None:intergenic	40.0%
	GAGGTCGATCAAAAGATGAA+AGG	-	Chr3:63509481-63509500	MsG0380014977.01.T01:CDS	40.0%
	GTATTCTTGAGGCAAAGCAT+TGG	-	Chr3:63509298-63509317	MsG0380014977.01.T01:CDS	40.0%
	TATTGCTGAGAATTTGAGGG+AGG	-	Chr3:63509462-63509481	MsG0380014977.01.T01:CDS	40.0%
!!	TGGATCAAGAGGTATTCTTG+AGG	-	Chr3:63509287-63509306	MsG0380014977.01.T01:CDS	40.0%
	AGTTTGGCCATCAGATTCAG+GGG	-	Chr3:63509204-63509223	MsG0380014977.01.T01:CDS	45.0%
	CAAGTTTGGCCATCAGATTC+AGG	-	Chr3:63509202-63509221	MsG0380014977.01.T01:CDS	45.0%
	CTTCTAACCCCTGAATCTGA+TGG	+	Chr3:63509214-63509233	None:intergenic	45.0%
!	GGGTTAGAAGTGTGATATCC+AGG	-	Chr3:63509224-63509243	MsG0380014977.01.T01:CDS	45.0%
	ACTCTTTGCGGAGTAGAAGC+CGG	-	Chr3:63509145-63509164	MsG0380014977.01.T01:CDS	50.0%
	GATGGCCAAACTTGTGCTTG+AGG	+	Chr3:63509196-63509215	None:intergenic	50.0%
	GTTCAGAAGTTTGCCTGGAC+TGG	-	Chr3:63509246-63509265	MsG0380014977.01.T01:CDS	50.0%
	TCCAGGCAAACTTCTGAACC+TGG	+	Chr3:63509245-63509264	None:intergenic	50.0%
	TCCAGGTTCAGAAGTTTGCC+TGG	-	Chr3:63509241-63509260	MsG0380014977.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	63509121	63509522	63509121	ID=MsG0380014977.01;Name=MsG0380014977.01
Chr3	mRNA	63509121	63509522	63509121	ID=MsG0380014977.01.T01;Parent=MsG0380014977.01;Name=MsG0380014977.01.T01;_AED=0.14;_eAED=0.14;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|133
Chr3	exon	63509121	63509522	63509121	ID=MsG0380014977.01.T01:exon:6686;Parent=MsG0380014977.01.T01
Chr3	CDS	63509121	63509522	63509121	ID=MsG0380014977.01.T01:cds;Parent=MsG0380014977.01.T01

Gene Sequence

>MsG0380014977.01.T01

ATGAAGAAGATTGATGAAATTAGCACTCTTTGCGGAGTAGAAGCCGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCACAAGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAGAAGTTTGCCTGGACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGTTGATGAAACAAAAGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACTACAGAATTTAATGGATATTTAATGAGCAAACATGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGTCAATCGAAGATTGA

Protein sequence

>MsG0380014977.01.T01

MKKIDEISTLCGVEAGAIIYEQNNPQAQVWPSDSGVRSVISRFRSLPGLEQSKKMVDQEVFLRQSIGKVYEQLMKQKKETRKQEMTNIIHHFIHTTEFNGYLMSKHDLNDLSSFIAENLREVDQKMKGMSIED*