Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001737.01.T01 | XP_013455733.1 | 67.327 | 202 | 55 | 3 | 1 | 197 | 1 | 196 | 9.05E-81 | 250 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001737.01.T01 | O64703 | 33.125 | 160 | 95 | 4 | 9 | 156 | 2 | 161 | 4.01E-17 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001737.01.T01 | A0A072UJ33 | 67.327 | 202 | 55 | 3 | 1 | 197 | 1 | 196 | 4.32e-81 | 250 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180001737.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180001737.01 | MsG0680034864.01 | PPI |
| MsG0180001737.01 | MsG0480020529.01 | PPI |
| MsG0180001737.01 | MsG0480021186.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001737.01.T01 | MTR_4g051538 | 67.327 | 202 | 55 | 3 | 1 | 197 | 1 | 196 | 1.10e-84 | 250 |
| MsG0180001737.01.T01 | MTR_3g104630 | 59.036 | 83 | 28 | 2 | 106 | 183 | 16 | 97 | 9.25e-24 | 92.4 |
| MsG0180001737.01.T01 | MTR_3g080940 | 50.685 | 73 | 36 | 0 | 1 | 73 | 1 | 73 | 1.15e-16 | 75.1 |
| MsG0180001737.01.T01 | MTR_3g052920 | 50.000 | 72 | 36 | 0 | 1 | 72 | 1 | 72 | 1.95e-16 | 75.1 |
| MsG0180001737.01.T01 | MTR_1g012570 | 44.048 | 84 | 42 | 1 | 1 | 79 | 1 | 84 | 2.77e-16 | 74.7 |
| MsG0180001737.01.T01 | MTR_1g106070 | 47.222 | 72 | 38 | 0 | 1 | 72 | 1 | 72 | 3.41e-16 | 73.6 |
| MsG0180001737.01.T01 | MTR_8g043650 | 47.222 | 72 | 38 | 0 | 1 | 72 | 1 | 72 | 6.23e-16 | 73.9 |
| MsG0180001737.01.T01 | MTR_2g085280 | 39.216 | 102 | 58 | 2 | 2 | 100 | 3 | 103 | 7.04e-16 | 73.6 |
| MsG0180001737.01.T01 | MTR_2g085250 | 39.216 | 102 | 58 | 2 | 2 | 100 | 3 | 103 | 7.04e-16 | 73.6 |
| MsG0180001737.01.T01 | MTR_1g105910 | 47.222 | 72 | 38 | 0 | 1 | 72 | 1 | 72 | 8.04e-16 | 73.6 |
| MsG0180001737.01.T01 | MTR_3g052870 | 47.222 | 72 | 38 | 0 | 1 | 72 | 1 | 72 | 9.13e-16 | 73.2 |
| MsG0180001737.01.T01 | MTR_8g086290 | 33.607 | 122 | 63 | 1 | 4 | 107 | 7 | 128 | 9.14e-16 | 72.0 |
| MsG0180001737.01.T01 | MTR_1g105920 | 47.222 | 72 | 38 | 0 | 1 | 72 | 1 | 72 | 1.35e-15 | 72.8 |
| MsG0180001737.01.T01 | MTR_1g108580 | 45.833 | 72 | 39 | 0 | 1 | 72 | 1 | 72 | 1.37e-15 | 72.8 |
| MsG0180001737.01.T01 | MTR_3g030770 | 44.444 | 72 | 40 | 0 | 1 | 72 | 1 | 72 | 1.45e-15 | 72.8 |
| MsG0180001737.01.T01 | MTR_1g108510 | 44.444 | 72 | 40 | 0 | 1 | 72 | 1 | 72 | 1.71e-15 | 72.4 |
| MsG0180001737.01.T01 | MTR_1g077360 | 45.833 | 72 | 39 | 0 | 1 | 72 | 1 | 72 | 1.75e-15 | 72.8 |
| MsG0180001737.01.T01 | MTR_2g030740 | 47.222 | 72 | 38 | 0 | 1 | 72 | 1 | 72 | 2.30e-15 | 72.4 |
| MsG0180001737.01.T01 | MTR_2g105290 | 38.667 | 75 | 46 | 0 | 4 | 78 | 7 | 81 | 3.97e-15 | 70.5 |
| MsG0180001737.01.T01 | MTR_5g053390 | 44.444 | 72 | 40 | 0 | 1 | 72 | 1 | 72 | 4.25e-15 | 71.6 |
| MsG0180001737.01.T01 | MTR_5g055100 | 30.147 | 136 | 84 | 3 | 1 | 126 | 1 | 135 | 1.02e-14 | 70.5 |
| MsG0180001737.01.T01 | MTR_5g045560 | 44.444 | 72 | 40 | 0 | 1 | 72 | 1 | 72 | 1.96e-14 | 69.7 |
| MsG0180001737.01.T01 | MTR_0121s0100 | 32.174 | 115 | 72 | 2 | 1 | 109 | 1 | 115 | 3.34e-14 | 67.0 |
| MsG0180001737.01.T01 | MTR_2g093190 | 43.056 | 72 | 41 | 0 | 1 | 72 | 1 | 72 | 5.51e-14 | 68.6 |
| MsG0180001737.01.T01 | MTR_4g094632 | 39.796 | 98 | 55 | 2 | 9 | 106 | 2 | 95 | 6.32e-14 | 67.0 |
| MsG0180001737.01.T01 | MTR_4g094638 | 43.478 | 69 | 39 | 0 | 9 | 77 | 2 | 70 | 6.91e-14 | 67.4 |
| MsG0180001737.01.T01 | MTR_3g030780 | 39.326 | 89 | 50 | 1 | 1 | 85 | 1 | 89 | 9.22e-14 | 65.9 |
| MsG0180001737.01.T01 | MTR_1g075600 | 41.667 | 72 | 42 | 0 | 1 | 72 | 1 | 72 | 1.44e-13 | 67.4 |
| MsG0180001737.01.T01 | MTR_8g079502 | 38.889 | 72 | 44 | 0 | 9 | 80 | 2 | 73 | 7.55e-13 | 64.3 |
| MsG0180001737.01.T01 | MTR_1g054265 | 40.000 | 70 | 42 | 0 | 9 | 78 | 2 | 71 | 7.71e-13 | 64.3 |
| MsG0180001737.01.T01 | MTR_8g046350 | 29.801 | 151 | 89 | 3 | 9 | 156 | 2 | 138 | 1.24e-12 | 63.5 |
| MsG0180001737.01.T01 | MTR_1g047550 | 38.889 | 72 | 44 | 0 | 9 | 80 | 2 | 73 | 1.47e-12 | 63.5 |
| MsG0180001737.01.T01 | MTR_0121s0080 | 28.834 | 163 | 88 | 4 | 5 | 148 | 5 | 158 | 4.52e-12 | 63.2 |
| MsG0180001737.01.T01 | MTR_7g028448 | 29.134 | 127 | 84 | 3 | 26 | 148 | 29 | 153 | 6.09e-12 | 61.6 |
| MsG0180001737.01.T01 | MTR_4g036915 | 29.134 | 127 | 84 | 3 | 26 | 148 | 29 | 153 | 6.09e-12 | 61.6 |
| MsG0180001737.01.T01 | MTR_8g051580 | 31.304 | 115 | 69 | 3 | 9 | 116 | 2 | 113 | 6.10e-12 | 62.0 |
| MsG0180001737.01.T01 | MTR_1g063160 | 37.500 | 72 | 45 | 0 | 9 | 80 | 2 | 73 | 8.10e-12 | 61.6 |
| MsG0180001737.01.T01 | MTR_4g108720 | 37.500 | 112 | 54 | 2 | 9 | 120 | 2 | 97 | 9.56e-12 | 63.2 |
| MsG0180001737.01.T01 | MTR_3g067875 | 50.980 | 51 | 25 | 0 | 22 | 72 | 5 | 55 | 4.70e-11 | 57.0 |
| MsG0180001737.01.T01 | MTR_4g131030 | 36.111 | 72 | 46 | 0 | 9 | 80 | 2 | 73 | 5.22e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001737.01.T01 | AT4G36590 | 35.119 | 168 | 92 | 6 | 5 | 157 | 3 | 168 | 3.96e-19 | 82.8 |
| MsG0180001737.01.T01 | AT1G29962 | 50.000 | 80 | 39 | 1 | 1 | 80 | 1 | 79 | 1.65e-18 | 79.7 |
| MsG0180001737.01.T01 | AT2G34440 | 33.125 | 160 | 95 | 4 | 9 | 156 | 2 | 161 | 4.09e-18 | 78.2 |
| MsG0180001737.01.T01 | AT1G28460 | 32.895 | 152 | 83 | 3 | 1 | 137 | 1 | 148 | 2.33e-16 | 73.9 |
| MsG0180001737.01.T01 | AT2G24840 | 45.000 | 80 | 44 | 0 | 7 | 86 | 61 | 140 | 3.69e-16 | 75.1 |
| MsG0180001737.01.T01 | AT1G28450 | 45.000 | 80 | 43 | 1 | 1 | 80 | 1 | 79 | 3.15e-14 | 68.2 |
| MsG0180001737.01.T01 | AT5G60440 | 47.059 | 68 | 36 | 0 | 5 | 72 | 3 | 70 | 5.64e-14 | 69.3 |
| MsG0180001737.01.T01 | AT1G01530 | 35.714 | 98 | 59 | 1 | 5 | 98 | 3 | 100 | 3.58e-13 | 66.6 |
| MsG0180001737.01.T01 | AT3G66656 | 44.615 | 65 | 36 | 0 | 9 | 73 | 2 | 66 | 3.93e-13 | 65.1 |
| MsG0180001737.01.T01 | AT1G65360 | 42.188 | 64 | 37 | 0 | 9 | 72 | 7 | 70 | 3.34e-12 | 63.5 |
| MsG0180001737.01.T01 | AT3G04100 | 39.130 | 69 | 42 | 0 | 5 | 73 | 10 | 78 | 5.66e-12 | 62.8 |
Find 29 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAGTTATCAACACTTTC+TGG | 0.133767 | 1:-26206265 | MsG0180001737.01.T01:CDS |
| GATGGTTCTCCATAACAAAA+TGG | 0.328060 | 1:+26206202 | None:intergenic |
| TTGAAGATACTGAAAAGTTC+TGG | 0.347202 | 1:-26206034 | MsG0180001737.01.T01:CDS |
| AAATCCTCCTTATGCCTTTG+AGG | 0.374783 | 1:-26205731 | MsG0180001737.01.T01:CDS |
| CTTCAAACTAGTTCTTGTAA+TGG | 0.376223 | 1:+26206171 | None:intergenic |
| CTGAGTTATCAACACTTTCT+GGG | 0.391775 | 1:-26206264 | MsG0180001737.01.T01:CDS |
| TACTGAAAAGTTCTGGCTAT+TGG | 0.398285 | 1:-26206027 | MsG0180001737.01.T01:CDS |
| CTTGTAATGGATTCAGAAGA+TGG | 0.408810 | 1:+26206184 | None:intergenic |
| CTTCCATTGAAGAACTGAAT+GGG | 0.415431 | 1:-26205762 | MsG0180001737.01.T01:CDS |
| TTTCCTCTTGATGTCCTCTT+CGG | 0.424395 | 1:+26206376 | None:intergenic |
| GAAATTCACACATCTTTAGT+TGG | 0.427085 | 1:-26205956 | MsG0180001737.01.T01:CDS |
| TGAAAATTCTTCAATAGAAG+AGG | 0.433445 | 1:-26206146 | MsG0180001737.01.T01:CDS |
| GACCCCATTCAGTTCTTCAA+TGG | 0.451368 | 1:+26205759 | None:intergenic |
| CATTACAAGAACTAGTTTGA+AGG | 0.460657 | 1:-26206170 | MsG0180001737.01.T01:CDS |
| TCTTCCATTGAAGAACTGAA+TGG | 0.465605 | 1:-26205763 | MsG0180001737.01.T01:CDS |
| ACCTAGGTTGTTGTCCTCAA+AGG | 0.526548 | 1:+26205717 | None:intergenic |
| TTGTTGTCCTCAAAGGCATA+AGG | 0.541240 | 1:+26205724 | None:intergenic |
| TTGAACCAACTTCATGACCT+AGG | 0.559040 | 1:+26205701 | None:intergenic |
| GAAAATTCTTCAATAGAAGA+GGG | 0.568036 | 1:-26206145 | MsG0180001737.01.T01:CDS |
| TGGCTATTGGTCGATTCAAG+AGG | 0.591337 | 1:-26206014 | MsG0180001737.01.T01:CDS |
| GGAGGAGAGATTGACTCTCA+TGG | 0.597639 | 1:-26205911 | MsG0180001737.01.T01:intron |
| TGAGTTATCAACACTTTCTG+GGG | 0.601370 | 1:-26206263 | MsG0180001737.01.T01:CDS |
| TTCCATTGAAGAACTGAATG+GGG | 0.615741 | 1:-26205761 | MsG0180001737.01.T01:CDS |
| AACAACCTAGGTCATGAAGT+TGG | 0.625294 | 1:-26205706 | MsG0180001737.01.T01:CDS |
| GCCTTTGAGGACAACAACCT+AGG | 0.634816 | 1:-26205718 | MsG0180001737.01.T01:CDS |
| TTGTCCTCAAAGGCATAAGG+AGG | 0.639612 | 1:+26205727 | None:intergenic |
| GGTCCGAAGAGGACATCAAG+AGG | 0.645593 | 1:-26206379 | MsG0180001737.01.T01:CDS |
| GTTGAATAAACTGCATGCTG+CGG | 0.678893 | 1:-26206074 | MsG0180001737.01.T01:CDS |
| AAAGTCTGGTATTTACAACA+AGG | 0.698329 | 1:-26206290 | MsG0180001737.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTAATTAATTTAAGTGAAAT+TGG | - | Chr1:26206250-26206269 | MsG0180001737.01.T01:CDS | 15.0% |
| !! | GTCTTAAATAAATTGAAAAA+AGG | - | Chr1:26206125-26206144 | MsG0180001737.01.T01:CDS | 15.0% |
| !! | TTAAATAAATTGAAAAAAGG+AGG | - | Chr1:26206128-26206147 | MsG0180001737.01.T01:CDS | 15.0% |
| !!! | TTTCTTTTACTTGATCATAT+TGG | + | Chr1:26206085-26206104 | None:intergenic | 20.0% |
| ! | GAAAATTCTTCAATAGAAGA+GGG | - | Chr1:26205912-26205931 | MsG0180001737.01.T01:CDS | 25.0% |
| ! | TGAAAATTCTTCAATAGAAG+AGG | - | Chr1:26205911-26205930 | MsG0180001737.01.T01:intron | 25.0% |
| AAAGTCTGGTATTTACAACA+AGG | - | Chr1:26205767-26205786 | MsG0180001737.01.T01:CDS | 30.0% | |
| GAAATTCACACATCTTTAGT+TGG | - | Chr1:26206101-26206120 | MsG0180001737.01.T01:CDS | 30.0% | |
| TCTTCTTACTAACTATGTGT+TGG | - | Chr1:26206179-26206198 | MsG0180001737.01.T01:CDS | 30.0% | |
| TTGAAGATACTGAAAAGTTC+TGG | - | Chr1:26206023-26206042 | MsG0180001737.01.T01:CDS | 30.0% | |
| ! | ACTGGAAAACCATTTTGTTA+TGG | - | Chr1:26205846-26205865 | MsG0180001737.01.T01:intron | 30.0% |
| ! | CATTACAAGAACTAGTTTGA+AGG | - | Chr1:26205887-26205906 | MsG0180001737.01.T01:intron | 30.0% |
| ! | CTTCAAACTAGTTCTTGTAA+TGG | + | Chr1:26205889-26205908 | None:intergenic | 30.0% |
| ! | TAACAAAATGGTTTTCCAGT+TGG | + | Chr1:26205846-26205865 | None:intergenic | 30.0% |
| CTGAGTTATCAACACTTTCT+GGG | - | Chr1:26205793-26205812 | MsG0180001737.01.T01:intron | 35.0% | |
| CTTCCATTGAAGAACTGAAT+GGG | - | Chr1:26206295-26206314 | MsG0180001737.01.T01:CDS | 35.0% | |
| GATGGTTCTCCATAACAAAA+TGG | + | Chr1:26205858-26205877 | None:intergenic | 35.0% | |
| TCTGAGTTATCAACACTTTC+TGG | - | Chr1:26205792-26205811 | MsG0180001737.01.T01:intron | 35.0% | |
| TCTTCCATTGAAGAACTGAA+TGG | - | Chr1:26206294-26206313 | MsG0180001737.01.T01:CDS | 35.0% | |
| TGAGTTATCAACACTTTCTG+GGG | - | Chr1:26205794-26205813 | MsG0180001737.01.T01:intron | 35.0% | |
| TTCCATTGAAGAACTGAATG+GGG | - | Chr1:26206296-26206315 | MsG0180001737.01.T01:CDS | 35.0% | |
| ! | ATTCTCATGTTTTCTCCAAC+TGG | - | Chr1:26205828-26205847 | MsG0180001737.01.T01:intron | 35.0% |
| ! | TACTGAAAAGTTCTGGCTAT+TGG | - | Chr1:26206030-26206049 | MsG0180001737.01.T01:CDS | 35.0% |
| !! | CTTGTAATGGATTCAGAAGA+TGG | + | Chr1:26205876-26205895 | None:intergenic | 35.0% |
| AAATCCTCCTTATGCCTTTG+AGG | - | Chr1:26206326-26206345 | MsG0180001737.01.T01:CDS | 40.0% | |
| AACAACCTAGGTCATGAAGT+TGG | - | Chr1:26206351-26206370 | MsG0180001737.01.T01:CDS | 40.0% | |
| GTTGAATAAACTGCATGCTG+CGG | - | Chr1:26205983-26206002 | MsG0180001737.01.T01:CDS | 40.0% | |
| TTGAACCAACTTCATGACCT+AGG | + | Chr1:26206359-26206378 | None:intergenic | 40.0% | |
| TTTCCTCTTGATGTCCTCTT+CGG | + | Chr1:26205684-26205703 | None:intergenic | 40.0% | |
| ! | TTGTTGTCCTCAAAGGCATA+AGG | + | Chr1:26206336-26206355 | None:intergenic | 40.0% |
| ACCTAGGTTGTTGTCCTCAA+AGG | + | Chr1:26206343-26206362 | None:intergenic | 45.0% | |
| GACCCCATTCAGTTCTTCAA+TGG | + | Chr1:26206301-26206320 | None:intergenic | 45.0% | |
| ! | GCGTTTTCAACGAGAAAGTC+TGG | - | Chr1:26205753-26205772 | MsG0180001737.01.T01:CDS | 45.0% |
| ! | TGGCTATTGGTCGATTCAAG+AGG | - | Chr1:26206043-26206062 | MsG0180001737.01.T01:CDS | 45.0% |
| ! | TTGTCCTCAAAGGCATAAGG+AGG | + | Chr1:26206333-26206352 | None:intergenic | 45.0% |
| ! | GCCTTTGAGGACAACAACCT+AGG | - | Chr1:26206339-26206358 | MsG0180001737.01.T01:CDS | 50.0% |
| ! | GGAGGAGAGATTGACTCTCA+TGG | - | Chr1:26206146-26206165 | MsG0180001737.01.T01:CDS | 50.0% |
| GGTCCGAAGAGGACATCAAG+AGG | - | Chr1:26205678-26205697 | MsG0180001737.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 26205675 | 26206404 | 26205675 | ID=MsG0180001737.01;Name=MsG0180001737.01 |
| Chr1 | mRNA | 26205675 | 26206404 | 26205675 | ID=MsG0180001737.01.T01;Parent=MsG0180001737.01;Name=MsG0180001737.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|202 |
| Chr1 | exon | 26205912 | 26206404 | 26205912 | ID=MsG0180001737.01.T01:exon:41063;Parent=MsG0180001737.01.T01 |
| Chr1 | exon | 26205675 | 26205790 | 26205675 | ID=MsG0180001737.01.T01:exon:41062;Parent=MsG0180001737.01.T01 |
| Chr1 | CDS | 26205912 | 26206404 | 26205912 | ID=MsG0180001737.01.T01:cds;Parent=MsG0180001737.01.T01 |
| Chr1 | CDS | 26205675 | 26205790 | 26205675 | ID=MsG0180001737.01.T01:cds;Parent=MsG0180001737.01.T01 |
| Gene Sequence |
| Protein sequence |
