Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034864.01.T01 | XP_013455733.1 | 71.837 | 245 | 51 | 8 | 1 | 238 | 1 | 234 | 1.89E-100 | 301 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034864.01.T01 | O64703 | 31.646 | 158 | 99 | 5 | 9 | 159 | 2 | 157 | 2.71E-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034864.01.T01 | A0A072UJ33 | 71.837 | 245 | 51 | 8 | 1 | 238 | 1 | 234 | 9.02e-101 | 301 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180001737.01 | MsG0680034864.01 | PPI |
| MsG0480020529.01 | MsG0680034864.01 | PPI |
| MsG0480021186.01 | MsG0680034864.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034864.01.T01 | MTR_4g051538 | 71.837 | 245 | 51 | 8 | 1 | 238 | 1 | 234 | 2.29e-104 | 301 |
| MsG0680034864.01.T01 | MTR_3g104630 | 45.185 | 135 | 59 | 6 | 106 | 230 | 16 | 145 | 1.83e-24 | 95.1 |
| MsG0680034864.01.T01 | MTR_3g052920 | 29.101 | 189 | 119 | 4 | 5 | 183 | 5 | 188 | 5.93e-17 | 77.4 |
| MsG0680034864.01.T01 | MTR_1g108510 | 29.560 | 159 | 98 | 3 | 5 | 149 | 5 | 163 | 2.58e-16 | 75.5 |
| MsG0680034864.01.T01 | MTR_8g043650 | 34.307 | 137 | 80 | 3 | 1 | 127 | 1 | 137 | 3.10e-16 | 75.5 |
| MsG0680034864.01.T01 | MTR_3g030770 | 29.448 | 163 | 101 | 3 | 1 | 149 | 1 | 163 | 6.20e-16 | 74.7 |
| MsG0680034864.01.T01 | MTR_1g106070 | 34.559 | 136 | 79 | 3 | 1 | 126 | 1 | 136 | 6.60e-16 | 73.6 |
| MsG0680034864.01.T01 | MTR_1g105910 | 35.338 | 133 | 76 | 3 | 5 | 127 | 5 | 137 | 6.71e-16 | 74.7 |
| MsG0680034864.01.T01 | MTR_1g108580 | 32.117 | 137 | 83 | 2 | 1 | 127 | 1 | 137 | 1.29e-15 | 73.6 |
| MsG0680034864.01.T01 | MTR_1g012570 | 45.588 | 68 | 37 | 0 | 5 | 72 | 5 | 72 | 2.10e-15 | 73.2 |
| MsG0680034864.01.T01 | MTR_3g052870 | 42.308 | 78 | 45 | 0 | 5 | 82 | 5 | 82 | 3.00e-15 | 72.4 |
| MsG0680034864.01.T01 | MTR_1g077360 | 40.244 | 82 | 49 | 0 | 1 | 82 | 1 | 82 | 3.48e-15 | 72.8 |
| MsG0680034864.01.T01 | MTR_1g105920 | 42.308 | 78 | 45 | 0 | 5 | 82 | 5 | 82 | 4.05e-15 | 72.4 |
| MsG0680034864.01.T01 | MTR_3g080940 | 42.683 | 82 | 47 | 0 | 1 | 82 | 1 | 82 | 4.15e-15 | 72.0 |
| MsG0680034864.01.T01 | MTR_2g030740 | 42.308 | 78 | 45 | 0 | 5 | 82 | 5 | 82 | 5.59e-15 | 72.0 |
| MsG0680034864.01.T01 | MTR_2g085250 | 40.260 | 77 | 46 | 0 | 1 | 77 | 1 | 77 | 6.44e-15 | 72.0 |
| MsG0680034864.01.T01 | MTR_2g085280 | 40.260 | 77 | 46 | 0 | 1 | 77 | 1 | 77 | 7.34e-15 | 71.6 |
| MsG0680034864.01.T01 | MTR_5g053390 | 39.744 | 78 | 47 | 0 | 5 | 82 | 5 | 82 | 1.67e-14 | 70.9 |
| MsG0680034864.01.T01 | MTR_5g055100 | 30.216 | 139 | 80 | 3 | 1 | 126 | 1 | 135 | 1.93e-14 | 70.5 |
| MsG0680034864.01.T01 | MTR_4g094632 | 35.294 | 102 | 62 | 1 | 9 | 106 | 2 | 103 | 2.63e-14 | 68.6 |
| MsG0680034864.01.T01 | MTR_8g086290 | 27.950 | 161 | 109 | 2 | 4 | 159 | 7 | 165 | 7.25e-14 | 67.8 |
| MsG0680034864.01.T01 | MTR_5g045560 | 40.260 | 77 | 46 | 0 | 1 | 77 | 1 | 77 | 8.49e-14 | 68.9 |
| MsG0680034864.01.T01 | MTR_2g093190 | 40.278 | 72 | 43 | 0 | 1 | 72 | 1 | 72 | 1.94e-13 | 67.8 |
| MsG0680034864.01.T01 | MTR_0121s0100 | 36.364 | 77 | 49 | 0 | 1 | 77 | 1 | 77 | 2.01e-13 | 65.5 |
| MsG0680034864.01.T01 | MTR_4g094638 | 38.571 | 70 | 43 | 0 | 9 | 78 | 2 | 71 | 2.51e-13 | 66.6 |
| MsG0680034864.01.T01 | MTR_1g075600 | 37.179 | 78 | 49 | 0 | 5 | 82 | 5 | 82 | 2.84e-13 | 67.4 |
| MsG0680034864.01.T01 | MTR_2g105290 | 25.625 | 160 | 112 | 2 | 4 | 158 | 7 | 164 | 7.17e-13 | 65.1 |
| MsG0680034864.01.T01 | MTR_3g030780 | 38.889 | 72 | 44 | 0 | 1 | 72 | 1 | 72 | 8.29e-13 | 63.9 |
| MsG0680034864.01.T01 | MTR_0121s0080 | 27.132 | 129 | 81 | 1 | 5 | 133 | 5 | 120 | 3.10e-12 | 64.7 |
| MsG0680034864.01.T01 | MTR_8g079502 | 33.735 | 83 | 47 | 1 | 9 | 83 | 2 | 84 | 3.13e-11 | 60.8 |
| MsG0680034864.01.T01 | MTR_1g047550 | 33.333 | 72 | 48 | 0 | 9 | 80 | 2 | 73 | 9.96e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034864.01.T01 | AT4G36590 | 32.353 | 170 | 98 | 5 | 5 | 159 | 3 | 170 | 2.24e-17 | 79.0 |
| MsG0680034864.01.T01 | AT2G34440 | 31.646 | 158 | 99 | 5 | 9 | 159 | 2 | 157 | 2.77e-16 | 74.3 |
| MsG0680034864.01.T01 | AT1G29962 | 43.750 | 80 | 44 | 1 | 1 | 80 | 1 | 79 | 1.00e-15 | 73.2 |
| MsG0680034864.01.T01 | AT5G60440 | 42.308 | 78 | 44 | 1 | 5 | 82 | 3 | 79 | 3.23e-14 | 70.9 |
| MsG0680034864.01.T01 | AT2G24840 | 40.000 | 80 | 48 | 0 | 7 | 86 | 61 | 140 | 5.00e-14 | 70.1 |
| MsG0680034864.01.T01 | AT1G28460 | 29.605 | 152 | 88 | 3 | 1 | 137 | 1 | 148 | 3.57e-13 | 66.2 |
| MsG0680034864.01.T01 | AT1G01530 | 33.645 | 107 | 67 | 1 | 5 | 107 | 3 | 109 | 1.03e-12 | 66.2 |
| MsG0680034864.01.T01 | AT3G04100 | 35.714 | 84 | 51 | 1 | 5 | 88 | 10 | 90 | 7.92e-12 | 63.2 |
| MsG0680034864.01.T01 | AT3G66656 | 40.000 | 65 | 39 | 0 | 9 | 73 | 2 | 66 | 2.45e-11 | 61.2 |
| MsG0680034864.01.T01 | AT1G28450 | 38.750 | 80 | 48 | 1 | 1 | 80 | 1 | 79 | 4.06e-11 | 60.5 |
Find 30 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGTATTGTCTTCCTTTCA+TGG | 0.211509 | 6:+95833994 | MsG0680034864.01.T01:CDS |
| TTGAAGATGTTGGAAAGTTC+TGG | 0.270990 | 6:+95833393 | MsG0680034864.01.T01:CDS |
| TCTGAGTTAACAACACTTTC+AGG | 0.273550 | 6:+95833162 | MsG0680034864.01.T01:CDS |
| GCAACCATACCTTTACCTTT+TGG | 0.288774 | 6:-95834033 | None:intergenic |
| TTTCCTCTTGATGACCTCTT+TGG | 0.311240 | 6:-95833051 | None:intergenic |
| ACACAAGGAGTCAACCTTAT+TGG | 0.323352 | 6:+95834078 | MsG0680034864.01.T01:CDS |
| ATCATTTCCTCTTCTATTGA+AGG | 0.331143 | 6:-95833288 | None:intergenic |
| ATCCTCCGCTTGCACCAATA+AGG | 0.357163 | 6:-95834092 | None:intergenic |
| TGTTGGAAAGTTCTGGATAT+TGG | 0.391346 | 6:+95833400 | MsG0680034864.01.T01:CDS |
| TTTATTTGTGACAGCAACTA+AGG | 0.402905 | 6:-95833484 | None:intergenic |
| ATTCTCATGCTTTCTCCAAC+GGG | 0.403336 | 6:+95833198 | MsG0680034864.01.T01:CDS |
| TTTCTTTCACTTGATCATAC+TGG | 0.408170 | 6:-95833452 | None:intergenic |
| TTTGTTATAGATTCAGAAGA+TGG | 0.410640 | 6:-95833243 | None:intergenic |
| CCTCTTGATGACCTCTTTGG+CGG | 0.444165 | 6:-95833048 | None:intergenic |
| GCATTTACAACGAGAAAGTC+TGG | 0.455029 | 6:+95833123 | MsG0680034864.01.T01:CDS |
| TATTCTCATGCTTTCTCCAA+CGG | 0.502164 | 6:+95833197 | MsG0680034864.01.T01:CDS |
| TGATCCAAAAGGTAAAGGTA+TGG | 0.511773 | 6:+95834029 | MsG0680034864.01.T01:CDS |
| TGAAAATCCTTCAATAGAAG+AGG | 0.547647 | 6:+95833281 | MsG0680034864.01.T01:CDS |
| GTCAACCTTATTGGTGCAAG+CGG | 0.586884 | 6:+95834087 | MsG0680034864.01.T01:CDS |
| AACCTTATTGGTGCAAGCGG+AGG | 0.596051 | 6:+95834090 | MsG0680034864.01.T01:CDS |
| GAGTAAGAATGCCGCCAAAG+AGG | 0.607370 | 6:+95833037 | None:intergenic |
| TTTGGATGAAAAGCATGTTG+CGG | 0.629913 | 6:+95833353 | MsG0680034864.01.T01:CDS |
| CCGCCAAAGAGGTCATCAAG+AGG | 0.664394 | 6:+95833048 | MsG0680034864.01.T01:CDS |
| TGAAAGTGTTGTTAACTCAG+AGG | 0.669188 | 6:-95833160 | None:intergenic |
| GGAGGAGATCTTAACCCTCG+TGG | 0.672202 | 6:+95833531 | MsG0680034864.01.T01:CDS |
| GCAAGCGGAGGATATGAAAG+TGG | 0.676676 | 6:+95834102 | MsG0680034864.01.T01:CDS |
| AAGCATGAGAATATCAACTG+AGG | 0.702546 | 6:-95833187 | None:intergenic |
| TCTCGTGATATAGAAACACA+AGG | 0.706006 | 6:+95834063 | MsG0680034864.01.T01:CDS |
| AAGATGTACACATACCACGA+GGG | 0.757349 | 6:-95833545 | None:intergenic |
| AAAGTCTGGAATTTACAACA+AGG | 0.757794 | 6:+95833137 | MsG0680034864.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAAAATAATTAGACAAAA+AGG | - | Chr6:95833628-95833647 | None:intergenic | 10.0% |
| !! | AAAAACAAAAAATTAAACAC+AGG | - | Chr6:95833780-95833799 | None:intergenic | 15.0% |
| !! | ATAAATAAAGTCTTGAAAAA+TGG | + | Chr6:95833510-95833529 | MsG0680034864.01.T01:CDS | 15.0% |
| !!! | CTAATTATTTTAAATGAATC+TGG | + | Chr6:95833634-95833653 | MsG0680034864.01.T01:intron | 15.0% |
| !! | AAAAAATTAAACACAGGAAT+GGG | - | Chr6:95833774-95833793 | None:intergenic | 20.0% |
| !! | AATATTAAATGGTAAAAGCT+GGG | - | Chr6:95833874-95833893 | None:intergenic | 20.0% |
| !! | ACCATTTAATATTCACTTAT+GGG | + | Chr6:95833881-95833900 | MsG0680034864.01.T01:intron | 20.0% |
| !! | TACCATTTAATATTCACTTA+TGG | + | Chr6:95833880-95833899 | MsG0680034864.01.T01:intron | 20.0% |
| !! | TCTTCTTAATTTCTATTACT+AGG | + | Chr6:95833922-95833941 | MsG0680034864.01.T01:intron | 20.0% |
| !!! | TCATTTTGCTGTATAATATT+CGG | - | Chr6:95833318-95833337 | None:intergenic | 20.0% |
| !!! | TGAAAAAAATGATGATCTTT+TGG | + | Chr6:95833335-95833354 | MsG0680034864.01.T01:CDS | 20.0% |
| ! | AAAAAGATTGAATCCATGAA+AGG | - | Chr6:95834010-95834029 | None:intergenic | 25.0% |
| ! | AATAAAGTCTTGAAAAATGG+AGG | + | Chr6:95833513-95833532 | MsG0680034864.01.T01:CDS | 25.0% |
| ! | ACGTAAAAATTTGAAGATGT+TGG | + | Chr6:95833383-95833402 | MsG0680034864.01.T01:CDS | 25.0% |
| ! | CAAAAAATTAAACACAGGAA+TGG | - | Chr6:95833775-95833794 | None:intergenic | 25.0% |
| ! | GAATATTAAATGGTAAAAGC+TGG | - | Chr6:95833875-95833894 | None:intergenic | 25.0% |
| ! | TCCCATAAGTGAATATTAAA+TGG | - | Chr6:95833885-95833904 | None:intergenic | 25.0% |
| !! | TTCAATCTTTTTGATCCAAA+AGG | + | Chr6:95834018-95834037 | MsG0680034864.01.T01:CDS | 25.0% |
| !! | TTTGTTATAGATTCAGAAGA+TGG | - | Chr6:95833246-95833265 | None:intergenic | 25.0% |
| !! | TTTTGCTTGAAATATTCTAG+CGG | + | Chr6:95833796-95833815 | MsG0680034864.01.T01:intron | 25.0% |
| !!! | AAATCCTACTTATGTTTTTG+AGG | + | Chr6:95833709-95833728 | MsG0680034864.01.T01:intron | 25.0% |
| AACAGCAAAGATCATGAAAT+TGG | + | Chr6:95833734-95833753 | MsG0680034864.01.T01:intron | 30.0% | |
| ATCAAGAGGAAAACAAAAAG+TGG | + | Chr6:95833062-95833081 | MsG0680034864.01.T01:CDS | 30.0% | |
| ATCATTTCCTCTTCTATTGA+AGG | - | Chr6:95833291-95833310 | None:intergenic | 30.0% | |
| TATGTATTGTCTTCCTTTCA+TGG | + | Chr6:95833994-95834013 | MsG0680034864.01.T01:CDS | 30.0% | |
| TGAAAATCCTTCAATAGAAG+AGG | + | Chr6:95833281-95833300 | MsG0680034864.01.T01:CDS | 30.0% | |
| TTGTCCTCAAAAACATAAGT+AGG | - | Chr6:95833716-95833735 | None:intergenic | 30.0% | |
| TTTATTTGTGACAGCAACTA+AGG | - | Chr6:95833487-95833506 | None:intergenic | 30.0% | |
| TTTCTTTCACTTGATCATAC+TGG | - | Chr6:95833455-95833474 | None:intergenic | 30.0% | |
| ! | AAAGTCTGGAATTTACAACA+AGG | + | Chr6:95833137-95833156 | MsG0680034864.01.T01:CDS | 30.0% |
| ! | CTTTTTGATCCAAAAGGTAA+AGG | + | Chr6:95834024-95834043 | MsG0680034864.01.T01:CDS | 30.0% |
| ! | GATGGTTTTCCATAACAAAA+TGG | - | Chr6:95833228-95833247 | None:intergenic | 30.0% |
| AAGCATGAGAATATCAACTG+AGG | - | Chr6:95833190-95833209 | None:intergenic | 35.0% | |
| TATTCTCATGCTTTCTCCAA+CGG | + | Chr6:95833197-95833216 | MsG0680034864.01.T01:CDS | 35.0% | |
| TCTCGTGATATAGAAACACA+AGG | + | Chr6:95834063-95834082 | MsG0680034864.01.T01:CDS | 35.0% | |
| TCTGAGTTAACAACACTTTC+AGG | + | Chr6:95833162-95833181 | MsG0680034864.01.T01:CDS | 35.0% | |
| TGATCCAAAAGGTAAAGGTA+TGG | + | Chr6:95834029-95834048 | MsG0680034864.01.T01:CDS | 35.0% | |
| TGTAGTAATTTCTACCATCG+AGG | + | Chr6:95833667-95833686 | MsG0680034864.01.T01:intron | 35.0% | |
| TTGAAGATGTTGGAAAGTTC+TGG | + | Chr6:95833393-95833412 | MsG0680034864.01.T01:CDS | 35.0% | |
| TTGTAGACAAGAGATGTTGT+TGG | + | Chr6:95833838-95833857 | MsG0680034864.01.T01:intron | 35.0% | |
| ! | ACGGGAAAACCATTTTGTTA+TGG | + | Chr6:95833216-95833235 | MsG0680034864.01.T01:CDS | 35.0% |
| ! | TAACAAAATGGTTTTCCCGT+TGG | - | Chr6:95833216-95833235 | None:intergenic | 35.0% |
| ! | TTTGGATGAAAAGCATGTTG+CGG | + | Chr6:95833353-95833372 | MsG0680034864.01.T01:CDS | 35.0% |
| !! | TGAAAGTGTTGTTAACTCAG+AGG | - | Chr6:95833163-95833182 | None:intergenic | 35.0% |
| !! | TGTTGGAAAGTTCTGGATAT+TGG | + | Chr6:95833400-95833419 | MsG0680034864.01.T01:CDS | 35.0% |
| AAGATGTACACATACCACGA+GGG | - | Chr6:95833548-95833567 | None:intergenic | 40.0% | |
| ACACAAGGAGTCAACCTTAT+TGG | + | Chr6:95834078-95834097 | MsG0680034864.01.T01:CDS | 40.0% | |
| ATTCTCATGCTTTCTCCAAC+GGG | + | Chr6:95833198-95833217 | MsG0680034864.01.T01:CDS | 40.0% | |
| GCATTTACAACGAGAAAGTC+TGG | + | Chr6:95833123-95833142 | MsG0680034864.01.T01:CDS | 40.0% | |
| ! | GCAACCATACCTTTACCTTT+TGG | - | Chr6:95834036-95834055 | None:intergenic | 40.0% |
| ! | TTTCCTCTTGATGACCTCTT+TGG | - | Chr6:95833054-95833073 | None:intergenic | 40.0% |
| GAAGATGTACACATACCACG+AGG | - | Chr6:95833549-95833568 | None:intergenic | 45.0% | |
| GTCAACCTTATTGGTGCAAG+CGG | + | Chr6:95834087-95834106 | MsG0680034864.01.T01:CDS | 45.0% | |
| TCTACCATCGAGGAACTGAA+TGG | + | Chr6:95833677-95833696 | MsG0680034864.01.T01:intron | 45.0% | |
| AACCTTATTGGTGCAAGCGG+AGG | + | Chr6:95834090-95834109 | MsG0680034864.01.T01:CDS | 50.0% | |
| ATCCTCCGCTTGCACCAATA+AGG | - | Chr6:95834095-95834114 | None:intergenic | 50.0% | |
| GACTCCATTCAGTTCCTCGA+TGG | - | Chr6:95833684-95833703 | None:intergenic | 50.0% | |
| GCAAGCGGAGGATATGAAAG+TGG | + | Chr6:95834102-95834121 | MsG0680034864.01.T01:CDS | 50.0% | |
| ! | CCTCTTGATGACCTCTTTGG+CGG | - | Chr6:95833051-95833070 | None:intergenic | 50.0% |
| CCGCCAAAGAGGTCATCAAG+AGG | + | Chr6:95833048-95833067 | MsG0680034864.01.T01:CDS | 55.0% | |
| GGAGGAGATCTTAACCCTCG+TGG | + | Chr6:95833531-95833550 | MsG0680034864.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 95833045 | 95834152 | 95833045 | ID=MsG0680034864.01;Name=MsG0680034864.01 |
| Chr6 | mRNA | 95833045 | 95834152 | 95833045 | ID=MsG0680034864.01.T01;Parent=MsG0680034864.01;Name=MsG0680034864.01.T01;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|238 |
| Chr6 | exon | 95833045 | 95833552 | 95833045 | ID=MsG0680034864.01.T01:exon:6556;Parent=MsG0680034864.01.T01 |
| Chr6 | exon | 95833944 | 95834152 | 95833944 | ID=MsG0680034864.01.T01:exon:6557;Parent=MsG0680034864.01.T01 |
| Chr6 | CDS | 95833045 | 95833552 | 95833045 | ID=MsG0680034864.01.T01:cds;Parent=MsG0680034864.01.T01 |
| Chr6 | CDS | 95833944 | 95834152 | 95833944 | ID=MsG0680034864.01.T01:cds;Parent=MsG0680034864.01.T01 |
| Gene Sequence |
| Protein sequence |
