AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002502.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002502.01.T01 MTR_1g051155 85.075 268 24 3 29 282 202 467 5.52e-159 451
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002502.01.T01 AT2G41020 56.180 267 102 5 29 282 199 463 9.69e-93 282
MsG0180002502.01.T01 AT2G41020 43.357 143 70 3 29 162 199 339 2.35e-29 115

Find 0 sgRNAs with CRISPR-Local

Find 319 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACAACATATAATTATAA+TGG - Chr1:39339528-39339547 None:intergenic 10.0%
!! AAACAATATATGTATATCAA+TGG - Chr1:39338668-39338687 None:intergenic 15.0%
!! AATATTATTCTGTAATCAAA+AGG + Chr1:39337390-39337409 MsG0180002502.01.T01:intron 15.0%
!! ACAAATTTACAATTTATTTC+AGG + Chr1:39340145-39340164 MsG0180002502.01.T01:intron 15.0%
!! ACTTACAAAATTCATAAAAA+TGG - Chr1:39338348-39338367 None:intergenic 15.0%
!! AGAATATATTGAAAAATCTA+GGG - Chr1:39340362-39340381 None:intergenic 15.0%
!! ATATTATTCTGTAATCAAAA+GGG + Chr1:39337391-39337410 MsG0180002502.01.T01:intron 15.0%
!! ATTGATATACATATATTGTT+TGG + Chr1:39338667-39338686 MsG0180002502.01.T01:intron 15.0%
!! CAAATTTACAATTTATTTCA+GGG + Chr1:39340146-39340165 MsG0180002502.01.T01:intron 15.0%
!! CTTACAAAATTCATAAAAAT+GGG - Chr1:39338347-39338366 None:intergenic 15.0%
!! TTACAAAATTCATAAAAATG+GGG - Chr1:39338346-39338365 None:intergenic 15.0%
!!! GAAATTTTTATTAATTAAGG+TGG - Chr1:39339657-39339676 None:intergenic 15.0%
!!! TATTATTATAATGAAAGTAC+CGG + Chr1:39337915-39337934 MsG0180002502.01.T01:CDS 15.0%
!!! TTAAGTTTTAAGAATAGATA+GGG + Chr1:39337278-39337297 MsG0180002502.01.T01:intron 15.0%
!! AACTCACTATTATATAAAAG+AGG - Chr1:39338926-39338945 None:intergenic 20.0%
!! ATAATTAATTAAGTCAGTAG+TGG + Chr1:39337251-39337270 MsG0180002502.01.T01:intron 20.0%
!! ATAATTTCAACTAATTAGAG+AGG - Chr1:39338827-39338846 None:intergenic 20.0%
!! ATTGATAAGGGAATAAAAAA+TGG + Chr1:39339961-39339980 MsG0180002502.01.T01:intron 20.0%
!! CTCTAATTAGTTGAAATTAT+GGG + Chr1:39338827-39338846 MsG0180002502.01.T01:intron 20.0%
!! GAGAATATATTGAAAAATCT+AGG - Chr1:39340363-39340382 None:intergenic 20.0%
!! TAAAAAAGGAGATATTCTAA+AGG - Chr1:39339256-39339275 None:intergenic 20.0%
!! TAACTCTTCTATACAAAATT+TGG - Chr1:39338778-39338797 None:intergenic 20.0%
!! TAATTAATTAAGTCAGTAGT+GGG + Chr1:39337252-39337271 MsG0180002502.01.T01:intron 20.0%
!! TAATTTCAACTAATTAGAGA+GGG - Chr1:39338826-39338845 None:intergenic 20.0%
!! TACAAAATTCATAAAAATGG+GGG - Chr1:39338345-39338364 None:intergenic 20.0%
!! TCTCTAATTAGTTGAAATTA+TGG + Chr1:39338826-39338845 MsG0180002502.01.T01:intron 20.0%
!! TGATATCGAAGAATAAAATA+GGG - Chr1:39337422-39337441 None:intergenic 20.0%
!! TTACTACAATACAAAAACTA+AGG + Chr1:39338408-39338427 MsG0180002502.01.T01:CDS 20.0%
!! TTGATAAGGGAATAAAAAAT+GGG + Chr1:39339962-39339981 MsG0180002502.01.T01:intron 20.0%
!!! ATTATTATAATGAAAGTACC+GGG + Chr1:39337916-39337935 MsG0180002502.01.T01:CDS 20.0%
!!! CTTTTATTATCAAAATGTCA+AGG + Chr1:39340402-39340421 MsG0180002502.01.T01:intron 20.0%
!!! GGGGAAATTTTTATTAATTA+AGG - Chr1:39339660-39339679 None:intergenic 20.0%
!!! GTTAAGTTTTAAGAATAGAT+AGG + Chr1:39337277-39337296 MsG0180002502.01.T01:intron 20.0%
!!! TAAGTTTTAAGAATAGATAG+GGG + Chr1:39337279-39337298 MsG0180002502.01.T01:intron 20.0%
!!! TACAAGCATATTTTCTTTTA+GGG + Chr1:39339329-39339348 MsG0180002502.01.T01:intron 20.0%
!!! TTACAAGCATATTTTCTTTT+AGG + Chr1:39339328-39339347 MsG0180002502.01.T01:intron 20.0%
!!! TTACTTTTTATTTTTGCTCA+AGG + Chr1:39338376-39338395 MsG0180002502.01.T01:intron 20.0%
!!! TTGATTGGTTAAAATTCAAT+GGG - Chr1:39338870-39338889 None:intergenic 20.0%
!!! TTTTTTATTCCCTTATCAAT+TGG - Chr1:39339961-39339980 None:intergenic 20.0%
! AAAAAGAAAGAATGTACGTA+CGG - Chr1:39340220-39340239 None:intergenic 25.0%
! AAACAAACTAACAAGAAGAT+AGG - Chr1:39340268-39340287 None:intergenic 25.0%
! ACCAAAACTATAATTATGAC+AGG - Chr1:39340605-39340624 None:intergenic 25.0%
! ACTATACATACTCTTGATAA+TGG + Chr1:39338129-39338148 MsG0180002502.01.T01:intron 25.0%
! AGTACAATACATTAAAATGG+TGG - Chr1:39339750-39339769 None:intergenic 25.0%
! AGTAGTTAATTATGCAGTTA+TGG + Chr1:39337207-39337226 MsG0180002502.01.T01:intron 25.0%
! ATGGGAGTAAAATTTGAATT+TGG - Chr1:39340774-39340793 None:intergenic 25.0%
! ATTGTGAAAATGAAAGTTGA+TGG + Chr1:39340456-39340475 MsG0180002502.01.T01:intron 25.0%
! ATTTACAATTTATTTCAGGG+TGG + Chr1:39340149-39340168 MsG0180002502.01.T01:intron 25.0%
! CAATATATGTATATCAATGG+CGG - Chr1:39338665-39338684 None:intergenic 25.0%
! CTGAGTACAATACATTAAAA+TGG - Chr1:39339753-39339772 None:intergenic 25.0%
! CTGATATCGAAGAATAAAAT+AGG - Chr1:39337423-39337442 None:intergenic 25.0%
! GTACAATACATTAAAATGGT+GGG - Chr1:39339749-39339768 None:intergenic 25.0%
! GTATTATAAATAAGATGCAG+AGG - Chr1:39337679-39337698 None:intergenic 25.0%
! TAACTTGATTATACGTTGAT+AGG + Chr1:39338710-39338729 MsG0180002502.01.T01:intron 25.0%
! TAGATTTGCAAAAATACTGT+AGG + Chr1:39339420-39339439 MsG0180002502.01.T01:intron 25.0%
! TTGTATTGAATCAAACACTA+GGG + Chr1:39339541-39339560 MsG0180002502.01.T01:intron 25.0%
!! ATTTTATCAGAATTTTCCCT+GGG - Chr1:39339083-39339102 None:intergenic 25.0%
!! GTCATTATACTTCAAGTTAT+GGG + Chr1:39337075-39337094 MsG0180002502.01.T01:CDS 25.0%
!! GTTGATTGGTTAAAATTCAA+TGG - Chr1:39338871-39338890 None:intergenic 25.0%
!! TAAATTTGTGTTTGCTAATC+AGG - Chr1:39340134-39340153 None:intergenic 25.0%
!! TAATCACTAATTTGTCATGT+AGG + Chr1:39337860-39337879 MsG0180002502.01.T01:intron 25.0%
!! TCCTGTCATAATTATAGTTT+TGG + Chr1:39340601-39340620 MsG0180002502.01.T01:intron 25.0%
!! TCTATCTCTAATATTTCTGT+TGG + Chr1:39338956-39338975 MsG0180002502.01.T01:intron 25.0%
!! TGTCATTATACTTCAAGTTA+TGG + Chr1:39337074-39337093 MsG0180002502.01.T01:CDS 25.0%
!! TTGTGAAAATGAAAGTTGAT+GGG + Chr1:39340457-39340476 MsG0180002502.01.T01:intron 25.0%
!! TTTTATTCTTCGATATCAGT+TGG + Chr1:39337424-39337443 MsG0180002502.01.T01:intron 25.0%
!!! TTCCTTTTTAATTATCACCT+CGG - Chr1:39339459-39339478 None:intergenic 25.0%
AATAATATTGCAGAACTTCG+AGG - Chr1:39337379-39337398 None:intergenic 30.0%
ACCAATCATCTAATTTACCA+AGG + Chr1:39338509-39338528 MsG0180002502.01.T01:CDS 30.0%
AGTAGGATACTGTTAGTAAT+AGG - Chr1:39337824-39337843 None:intergenic 30.0%
ATAATATTGCAGAACTTCGA+GGG - Chr1:39337378-39337397 None:intergenic 30.0%
ATTAATTAAGGTGGCACTAA+CGG - Chr1:39339648-39339667 None:intergenic 30.0%
ATTCATAAAAATGGGGGAAA+AGG - Chr1:39338339-39338358 None:intergenic 30.0%
CAGGGAAAATTCTGATAAAA+TGG + Chr1:39339082-39339101 MsG0180002502.01.T01:CDS 30.0%
CTAGGATGTTGTACAATTAA+AGG + Chr1:39338233-39338252 MsG0180002502.01.T01:intron 30.0%
CTTGATACTAACAACACTTT+AGG - Chr1:39340525-39340544 None:intergenic 30.0%
GGAAGGATATAAAACAGAAT+GGG - Chr1:39339848-39339867 None:intergenic 30.0%
GTTGTATTGAATCAAACACT+AGG + Chr1:39339540-39339559 MsG0180002502.01.T01:intron 30.0%
TATTTATTAAGCAGTAGCCT+AGG + Chr1:39338177-39338196 MsG0180002502.01.T01:intron 30.0%
TGTATTGAATCAAACACTAG+GGG + Chr1:39339542-39339561 MsG0180002502.01.T01:intron 30.0%
TTCATAAAAATGGGGGAAAA+GGG - Chr1:39338338-39338357 None:intergenic 30.0%
TTCCGAGGTGATAATTAAAA+AGG + Chr1:39339454-39339473 MsG0180002502.01.T01:intron 30.0%
TTGATATCAGTTTCTATCAC+TGG - Chr1:39337523-39337542 None:intergenic 30.0%
TTTAAATGGGCAATCAACAA+CGG + Chr1:39337971-39337990 MsG0180002502.01.T01:CDS 30.0%
TTTATTCCCTTATCAATTGG+AGG - Chr1:39339958-39339977 None:intergenic 30.0%
! AAAGGAGATATTCTAAAGGA+TGG - Chr1:39339252-39339271 None:intergenic 30.0%
! AAGAGAGTACGATGTAAAAA+AGG - Chr1:39339270-39339289 None:intergenic 30.0%
! CAAAAACTAAGGTATCACAA+TGG + Chr1:39338419-39338438 MsG0180002502.01.T01:CDS 30.0%
! CATTTTATCAGAATTTTCCC+TGG - Chr1:39339084-39339103 None:intergenic 30.0%
! GTGAAAAGCAATAGTTTTTC+TGG - Chr1:39339162-39339181 None:intergenic 30.0%
! TTGCTGATTAATCAAATTCG+AGG - Chr1:39339045-39339064 None:intergenic 30.0%
! TTTCCTTTTAAATTGGGGAA+GGG - Chr1:39339680-39339699 None:intergenic 30.0%
! TTTGTAATTTGCCATGCTTT+GGG + Chr1:39339192-39339211 MsG0180002502.01.T01:intron 30.0%
!! ATTTGGTTTCTTTTTCTGAG+AGG - Chr1:39340757-39340776 None:intergenic 30.0%
!! CTCTTACTTTATCGATTTCA+GGG + Chr1:39340666-39340685 MsG0180002502.01.T01:intron 30.0%
!! GGCTTTGAAAGAATAATGTT+AGG - Chr1:39339887-39339906 None:intergenic 30.0%
!! TAGGATTTTCTTTATTTCCG+AGG + Chr1:39339439-39339458 MsG0180002502.01.T01:intron 30.0%
!! TCTCTTACTTTATCGATTTC+AGG + Chr1:39340665-39340684 MsG0180002502.01.T01:intron 30.0%
!! TTTGAATTCGTTTTTCCACA+TGG - Chr1:39339398-39339417 None:intergenic 30.0%
!!! TTTTCTTTTAGGGTTACGTT+AGG + Chr1:39339339-39339358 MsG0180002502.01.T01:intron 30.0%
AAAGAATTCCTACAACCATG+TGG + Chr1:39339380-39339399 MsG0180002502.01.T01:intron 35.0%
AAATGATCAAATGCAACAGC+AGG - Chr1:39338062-39338081 None:intergenic 35.0%
AATTCCAAATCACTACCTTG+AGG - Chr1:39339601-39339620 None:intergenic 35.0%
AATTTGGATCACAACTAACG+AGG - Chr1:39338762-39338781 None:intergenic 35.0%
ACAACTTCCTGAGTAGAATT+AGG - Chr1:39338451-39338470 None:intergenic 35.0%
ACAATTTATTTCAGGGTGGT+GGG + Chr1:39340153-39340172 MsG0180002502.01.T01:intron 35.0%
AGGGAAAGAAATTCACAGTA+GGG - Chr1:39337480-39337499 None:intergenic 35.0%
AGTATGTGCTAGTAAACAGT+AGG - Chr1:39337841-39337860 None:intergenic 35.0%
ATAAAATGGCTAGCAAACAG+AGG + Chr1:39339096-39339115 MsG0180002502.01.T01:CDS 35.0%
ATTGGGATAAAGGATAGGTA+GGG - Chr1:39337637-39337656 None:intergenic 35.0%
CAATGGGTCCTAAATTTAGT+GGG - Chr1:39338854-39338873 None:intergenic 35.0%
CATCTAATTTACCAAGGCAT+GGG + Chr1:39338515-39338534 MsG0180002502.01.T01:CDS 35.0%
CCTAGAGTATATAACTTCCT+AGG - Chr1:39338197-39338216 None:intergenic 35.0%
CCTAGGAAGTTATATACTCT+AGG + Chr1:39338194-39338213 MsG0180002502.01.T01:intron 35.0%
GACTTCTATGTAACAGCTAT+AGG - Chr1:39337707-39337726 None:intergenic 35.0%
GAGGATCAATATGTTCTCTA+TGG - Chr1:39339908-39339927 None:intergenic 35.0%
GCAGAAGTTATAGATACTAG+TGG - Chr1:39337791-39337810 None:intergenic 35.0%
GCCTTGGTAAATTAGATGAT+TGG - Chr1:39338513-39338532 None:intergenic 35.0%
GGAGTAGTAGTAATCAAACT+AGG + Chr1:39338215-39338234 MsG0180002502.01.T01:intron 35.0%
GGGAAGGATATAAAACAGAA+TGG - Chr1:39339849-39339868 None:intergenic 35.0%
GTTAGTAATAGGACTCCTAA+GGG - Chr1:39337813-39337832 None:intergenic 35.0%
TAAGATGCAGAGGAAAAGAA+AGG - Chr1:39337669-39337688 None:intergenic 35.0%
TACAATTTATTTCAGGGTGG+TGG + Chr1:39340152-39340171 MsG0180002502.01.T01:intron 35.0%
TATCTATAACTTCTGCCCTT+AGG + Chr1:39337795-39337814 MsG0180002502.01.T01:intron 35.0%
TCAATGGGTCCTAAATTTAG+TGG - Chr1:39338855-39338874 None:intergenic 35.0%
TCATCTAATTTACCAAGGCA+TGG + Chr1:39338514-39338533 MsG0180002502.01.T01:CDS 35.0%
TCATGAAGAAGATGATGAGT+TGG + Chr1:39339997-39340016 MsG0180002502.01.T01:CDS 35.0%
TGGAAACCTCCAATTGATAA+GGG + Chr1:39339949-39339968 MsG0180002502.01.T01:intron 35.0%
TGTTAGTAATAGGACTCCTA+AGG - Chr1:39337814-39337833 None:intergenic 35.0%
TTATGATATCCTTCACCTTG+AGG + Chr1:39337112-39337131 MsG0180002502.01.T01:CDS 35.0%
TTATGGGTCCCACTAAATTT+AGG + Chr1:39338843-39338862 MsG0180002502.01.T01:intron 35.0%
TTCAGCGTTCTTTCGTAAAA+CGG - Chr1:39340730-39340749 None:intergenic 35.0%
TTGAAAAATCTAGGGATACC+AGG - Chr1:39340354-39340373 None:intergenic 35.0%
TTGTTAGTATCAAGCGTCAT+TGG + Chr1:39340531-39340550 MsG0180002502.01.T01:intron 35.0%
TTTGTGTCTTCCAGATAGTT+GGG + Chr1:39337995-39338014 MsG0180002502.01.T01:CDS 35.0%
! ATTTTACTCCCATATCCAAG+AGG + Chr1:39340781-39340800 MsG0180002502.01.T01:CDS 35.0%
! CATTTTACAACCATGTCTGA+GGG - Chr1:39339624-39339643 None:intergenic 35.0%
! CTTTCCTTTTAAATTGGGGA+AGG - Chr1:39339681-39339700 None:intergenic 35.0%
! CTTTGTAATTTGCCATGCTT+TGG + Chr1:39339191-39339210 MsG0180002502.01.T01:intron 35.0%
! TAGAGAGGGTGTGTTAAATA+GGG - Chr1:39338812-39338831 None:intergenic 35.0%
! TCACTAATTTGTCATGTAGG+TGG + Chr1:39337863-39337882 MsG0180002502.01.T01:intron 35.0%
! TTAATTAAGTCAGTAGTGGG+AGG + Chr1:39337255-39337274 MsG0180002502.01.T01:intron 35.0%
! TTAGAGAGGGTGTGTTAAAT+AGG - Chr1:39338813-39338832 None:intergenic 35.0%
! TTCCTTTTAAATTGGGGAAG+GGG - Chr1:39339679-39339698 None:intergenic 35.0%
! TTTTACTCCCATATCCAAGA+GGG + Chr1:39340782-39340801 MsG0180002502.01.T01:CDS 35.0%
! TTTTGTGTCTTCCAGATAGT+TGG + Chr1:39337994-39338013 MsG0180002502.01.T01:CDS 35.0%
!! AGTTTAGTCTTGTAACCTCA+AGG + Chr1:39339583-39339602 MsG0180002502.01.T01:intron 35.0%
!! GATCCTCATTTTTCTATCCA+GGG + Chr1:39339921-39339940 MsG0180002502.01.T01:intron 35.0%
!! TGAAAGTTGATGGGTAGAAT+TGG + Chr1:39340466-39340485 MsG0180002502.01.T01:intron 35.0%
!! TGATCCTCATTTTTCTATCC+AGG + Chr1:39339920-39339939 MsG0180002502.01.T01:intron 35.0%
!!! TGCTTACATAGGACTAGTTT+TGG + Chr1:39338615-39338634 MsG0180002502.01.T01:intron 35.0%
!!! TTACCCTGTTTTTTCACAAG+AGG + Chr1:39339775-39339794 MsG0180002502.01.T01:intron 35.0%
AAAATCTAGGGATACCAGGT+TGG - Chr1:39340350-39340369 None:intergenic 40.0%
AAAGATTCACCTTGTCCTCA+AGG - Chr1:39337130-39337149 None:intergenic 40.0%
AAGAATAGATAGGGGAGAGA+AGG + Chr1:39337287-39337306 MsG0180002502.01.T01:intron 40.0%
ACAGCCTCTTGTGAAAAAAC+AGG - Chr1:39339782-39339801 None:intergenic 40.0%
ACCAAGACTCTCGAATTTCT+TGG - Chr1:39337579-39337598 None:intergenic 40.0%
AGAATAGATAGGGGAGAGAA+GGG + Chr1:39337288-39337307 MsG0180002502.01.T01:intron 40.0%
AGGACAAGGTGAATCTTTAG+GGG + Chr1:39337132-39337151 MsG0180002502.01.T01:intron 40.0%
CAGCCTCTTGTGAAAAAACA+GGG - Chr1:39339781-39339800 None:intergenic 40.0%
CAGGGAAAGAAATTCACAGT+AGG - Chr1:39337481-39337500 None:intergenic 40.0%
CCAAGACTCTCGAATTTCTT+GGG - Chr1:39337578-39337597 None:intergenic 40.0%
CCATTTAAAGATGCTTCACG+GGG - Chr1:39337960-39337979 None:intergenic 40.0%
CCCATTTAAAGATGCTTCAC+GGG - Chr1:39337961-39337980 None:intergenic 40.0%
CCTCAGACATGGTTGTAAAA+TGG + Chr1:39339622-39339641 MsG0180002502.01.T01:intron 40.0%
CTAAAAGAATGCATCCAACC+TGG + Chr1:39340333-39340352 MsG0180002502.01.T01:intron 40.0%
CTACTGTGAATTTCTTTCCC+TGG + Chr1:39337479-39337498 MsG0180002502.01.T01:intron 40.0%
CTGGAAACCTCCAATTGATA+AGG + Chr1:39339948-39339967 MsG0180002502.01.T01:intron 40.0%
GAAAGAAATTCACAGTAGGG+GGG - Chr1:39337477-39337496 None:intergenic 40.0%
GAGGACAAATACTGACATTG+TGG - Chr1:39339026-39339045 None:intergenic 40.0%
GAGGACAAGGTGAATCTTTA+GGG + Chr1:39337131-39337150 MsG0180002502.01.T01:intron 40.0%
GATATCATAAGTGTCCACCT+GGG - Chr1:39337102-39337121 None:intergenic 40.0%
GATTGGGATAAAGGATAGGT+AGG - Chr1:39337638-39337657 None:intergenic 40.0%
GCCCATTTAAAGATGCTTCA+CGG - Chr1:39337962-39337981 None:intergenic 40.0%
GGAAAGAAATTCACAGTAGG+GGG - Chr1:39337478-39337497 None:intergenic 40.0%
GGGAAAGAAATTCACAGTAG+GGG - Chr1:39337479-39337498 None:intergenic 40.0%
GTAACCTCAAGGTAGTGATT+TGG + Chr1:39339594-39339613 MsG0180002502.01.T01:intron 40.0%
GTTGCAACTCTTGCTTACAT+AGG + Chr1:39338604-39338623 MsG0180002502.01.T01:intron 40.0%
TAAGGTCGTTGTTGAAACAG+AGG - Chr1:39340693-39340712 None:intergenic 40.0%
TAATAAGATGCACCACTTCC+CGG - Chr1:39337900-39337919 None:intergenic 40.0%
TAGCCCTGGATAGAAAAATG+AGG - Chr1:39339927-39339946 None:intergenic 40.0%
TAGGCAAGCTTTGCATCAAA+TGG - Chr1:39340506-39340525 None:intergenic 40.0%
TAGTCGCTACATGACTATGA+AGG - Chr1:39338739-39338758 None:intergenic 40.0%
TGAGGACAAGGTGAATCTTT+AGG + Chr1:39337130-39337149 MsG0180002502.01.T01:intron 40.0%
TTCCCCTTCCCCAATTTAAA+AGG + Chr1:39339674-39339693 MsG0180002502.01.T01:intron 40.0%
TTCTACTCAGGAAGTTGTGT+CGG + Chr1:39338453-39338472 MsG0180002502.01.T01:CDS 40.0%
TTTACTCCCATATCCAAGAG+GGG + Chr1:39340783-39340802 MsG0180002502.01.T01:CDS 40.0%
! ACGGGGCTTTCCTTTTAAAT+TGG - Chr1:39339687-39339706 None:intergenic 40.0%
! AGATGATGAGTTGGATCCTA+TGG + Chr1:39340006-39340025 MsG0180002502.01.T01:CDS 40.0%
! ATTTGGAATTCCCTCAGACA+TGG + Chr1:39339611-39339630 MsG0180002502.01.T01:intron 40.0%
! CCATTTTACAACCATGTCTG+AGG - Chr1:39339625-39339644 None:intergenic 40.0%
! CCCGTGAAGCATCTTTAAAT+GGG + Chr1:39337958-39337977 MsG0180002502.01.T01:CDS 40.0%
! CGGGGCTTTCCTTTTAAATT+GGG - Chr1:39339686-39339705 None:intergenic 40.0%
! GAAGCATCCTAATTCTACTC+AGG + Chr1:39338441-39338460 MsG0180002502.01.T01:CDS 40.0%
! GGGGCTTTCCTTTTAAATTG+GGG - Chr1:39339685-39339704 None:intergenic 40.0%
! GTATATCAATGGCGGAAAAC+AGG - Chr1:39338657-39338676 None:intergenic 40.0%
!! AAAGAAAGGCATTCAGGGAT+TGG - Chr1:39337655-39337674 None:intergenic 40.0%
!! AAGAAAGGCATTCAGGGATT+GGG - Chr1:39337654-39337673 None:intergenic 40.0%
!! AAGTTGATGGGTAGAATTGG+TGG + Chr1:39340469-39340488 MsG0180002502.01.T01:intron 40.0%
!! AGAGGAAAAGAAAGGCATTC+AGG - Chr1:39337661-39337680 None:intergenic 40.0%
!! GAGGAAAAGAAAGGCATTCA+GGG - Chr1:39337660-39337679 None:intergenic 40.0%
!! TCGTTTTTCCACATGGTTGT+AGG - Chr1:39339391-39339410 None:intergenic 40.0%
!!! TTTTTCCCATTGACTCTTCC+CGG - Chr1:39337937-39337956 None:intergenic 40.0%
AAGCTCTCGAATTCTTGGAG+GGG - Chr1:39337607-39337626 None:intergenic 45.0%
AGACCAAGCTCTCGAATTCT+TGG - Chr1:39337612-39337631 None:intergenic 45.0%
ATCCTTCCCCGCATTACTAA+CGG + Chr1:39339860-39339879 MsG0180002502.01.T01:intron 45.0%
ATCTGAGGATTTGACTGGGA+AGG + Chr1:39337324-39337343 MsG0180002502.01.T01:intron 45.0%
ATGGCTAGCAAACAGAGGTA+TGG + Chr1:39339101-39339120 MsG0180002502.01.T01:intron 45.0%
CAAGCTCTCGAATTCTTGGA+GGG - Chr1:39337608-39337627 None:intergenic 45.0%
CAGGGATTGGGATAAAGGAT+AGG - Chr1:39337642-39337661 None:intergenic 45.0%
CAGTAACTCATGCTTGCACA+AGG - Chr1:39338562-39338581 None:intergenic 45.0%
CCCAAGAAATTCGAGAGTCT+TGG + Chr1:39337575-39337594 MsG0180002502.01.T01:intron 45.0%
CTCAACACACACTCGTTGAT+TGG - Chr1:39338885-39338904 None:intergenic 45.0%
GACCTTTCTCAAGCTCAACA+TGG + Chr1:39340633-39340652 MsG0180002502.01.T01:intron 45.0%
GCATTCAGGGATTGGGATAA+AGG - Chr1:39337647-39337666 None:intergenic 45.0%
GGACAAGGTGAATCTTTAGG+GGG + Chr1:39337133-39337152 MsG0180002502.01.T01:intron 45.0%
GGATATCATAAGTGTCCACC+TGG - Chr1:39337103-39337122 None:intergenic 45.0%
GTAGGTGGAAGCAAAAGATC+CGG + Chr1:39337878-39337897 MsG0180002502.01.T01:intron 45.0%
GTTCAAGTCTCGTGACTGAA+AGG - Chr1:39339826-39339845 None:intergenic 45.0%
TAGGTGGAAGCAAAAGATCC+GGG + Chr1:39337879-39337898 MsG0180002502.01.T01:intron 45.0%
TATCCTTCACCTTGAGGACA+AGG + Chr1:39337118-39337137 MsG0180002502.01.T01:CDS 45.0%
TCTGAGGATTTGACTGGGAA+GGG + Chr1:39337325-39337344 MsG0180002502.01.T01:intron 45.0%
TGCCATGTTGAGCTTGAGAA+AGG - Chr1:39340638-39340657 None:intergenic 45.0%
TGTTAGGCCCGTTAGTAATG+CGG - Chr1:39339871-39339890 None:intergenic 45.0%
TTCTTTCGTAAAACGGCTCC+AGG - Chr1:39340723-39340742 None:intergenic 45.0%
! ACAAGGTGAATCTTTAGGGG+GGG + Chr1:39337135-39337154 MsG0180002502.01.T01:intron 45.0%
! ATCAGAGCAGTTCTGATCCA+GGG - Chr1:39337499-39337518 None:intergenic 45.0%
! CCCCGTGAAGCATCTTTAAA+TGG + Chr1:39337957-39337976 MsG0180002502.01.T01:CDS 45.0%
! GACAAGGTGAATCTTTAGGG+GGG + Chr1:39337134-39337153 MsG0180002502.01.T01:intron 45.0%
! TACTTCAAGTTATGGGACCC+AGG + Chr1:39337082-39337101 MsG0180002502.01.T01:CDS 45.0%
! TATCAGAGCAGTTCTGATCC+AGG - Chr1:39337500-39337519 None:intergenic 45.0%
! TCAGAATTTTCCCTGGGATG+TGG - Chr1:39339077-39339096 None:intergenic 45.0%
! TGACTGGTGTTCAGAAGCAT+AGG + Chr1:39340560-39340579 MsG0180002502.01.T01:intron 45.0%
! TTTCACAAGAGGCTGTTTCC+AGG + Chr1:39339786-39339805 MsG0180002502.01.T01:intron 45.0%
!! AAAGTACCGGGAAGAGTCAA+TGG + Chr1:39337928-39337947 MsG0180002502.01.T01:CDS 45.0%
!! AAGGGGCCATTTTGGCATTA+GGG + Chr1:39337343-39337362 MsG0180002502.01.T01:intron 45.0%
!! AAGTACCGGGAAGAGTCAAT+GGG + Chr1:39337929-39337948 MsG0180002502.01.T01:CDS 45.0%
!! TTTAGGGGGGGAGTATTGTT+AGG + Chr1:39337147-39337166 MsG0180002502.01.T01:intron 45.0%
!! TTTTTCTATCCAGGGCTAGC+TGG + Chr1:39339929-39339948 MsG0180002502.01.T01:intron 45.0%
!! AAAATAAAATAAAATGAAAA+AGG + Chr1:39339486-39339505 MsG0180002502.01.T01:intron 5.0%
AAGGTCACGAGTTCAAGTCC+TGG - Chr1:39339807-39339826 None:intergenic 50.0%
AATGCCTCCACCCAACTATC+TGG - Chr1:39338008-39338027 None:intergenic 50.0%
AGAGAGTCATGCCCAAAGCA+TGG - Chr1:39339206-39339225 None:intergenic 50.0%
ATGCGGGATCTTTAGTGCAC+GGG - Chr1:39339705-39339724 None:intergenic 50.0%
CAACGACCTTATCCATCACC+TGG + Chr1:39340702-39340721 MsG0180002502.01.T01:CDS 50.0%
CATGCGGGATCTTTAGTGCA+CGG - Chr1:39339706-39339725 None:intergenic 50.0%
CCAAGCTCTCGAATTCTTGG+AGG - Chr1:39337609-39337628 None:intergenic 50.0%
CCTCCAAGAATTCGAGAGCT+TGG + Chr1:39337606-39337625 MsG0180002502.01.T01:intron 50.0%
CTGAGGATTTGACTGGGAAG+GGG + Chr1:39337326-39337345 MsG0180002502.01.T01:intron 50.0%
GAAGCAAAAGATCCGGGAAG+TGG + Chr1:39337885-39337904 MsG0180002502.01.T01:CDS 50.0%
GAGCATCTGAATAGGAGCTG+GGG - Chr1:39340033-39340052 None:intergenic 50.0%
GATCAGGTGGTCTCATCAGT+TGG + Chr1:39338475-39338494 MsG0180002502.01.T01:CDS 50.0%
GCACCTCCCTAATGCCAAAA+TGG - Chr1:39337352-39337371 None:intergenic 50.0%
GGAGAGAAGGGAAGATTGAG+GGG + Chr1:39337300-39337319 MsG0180002502.01.T01:intron 50.0%
GGGAGAGAAGGGAAGATTGA+GGG + Chr1:39337299-39337318 MsG0180002502.01.T01:intron 50.0%
GGGCATCTGAGGATTTGACT+GGG + Chr1:39337320-39337339 MsG0180002502.01.T01:intron 50.0%
GGTCTCATCAGTTGGTCATC+TGG + Chr1:39338483-39338502 MsG0180002502.01.T01:CDS 50.0%
GTGTCTTCCAGATAGTTGGG+TGG + Chr1:39337998-39338017 MsG0180002502.01.T01:CDS 50.0%
GTTACTGCAGTCACTGCACA+AGG + Chr1:39338575-39338594 MsG0180002502.01.T01:CDS 50.0%
GTTAGGCCCGTTAGTAATGC+GGG - Chr1:39339870-39339889 None:intergenic 50.0%
TCACCTTGTCCTCAAGGTGA+AGG - Chr1:39337124-39337143 None:intergenic 50.0%
TCAGGAAGTTGTGTCGGATC+AGG + Chr1:39338459-39338478 MsG0180002502.01.T01:CDS 50.0%
TCCTATTCAGATGCTCCTCG+CGG + Chr1:39340037-39340056 MsG0180002502.01.T01:CDS 50.0%
TCCTTCCCCGCATTACTAAC+GGG + Chr1:39339861-39339880 MsG0180002502.01.T01:intron 50.0%
TCTTCCAGATAGTTGGGTGG+AGG + Chr1:39338001-39338020 MsG0180002502.01.T01:CDS 50.0%
TTAGGCCCGTTAGTAATGCG+GGG - Chr1:39339869-39339888 None:intergenic 50.0%
! AGATAGTTGGGTGGAGGCAT+TGG + Chr1:39338007-39338026 MsG0180002502.01.T01:CDS 50.0%
! ATTTACCAAGGCATGGGCTG+TGG + Chr1:39338521-39338540 MsG0180002502.01.T01:CDS 50.0%
! GCGTCATTGGCTGTTATGAC+TGG + Chr1:39340544-39340563 MsG0180002502.01.T01:intron 50.0%
! GGAGCATCTGAATAGGAGCT+GGG - Chr1:39340034-39340053 None:intergenic 50.0%
! TTCAAGTTATGGGACCCAGG+TGG + Chr1:39337085-39337104 MsG0180002502.01.T01:CDS 50.0%
!! AGGAGCATCTGAATAGGAGC+TGG - Chr1:39340035-39340054 None:intergenic 50.0%
!! GAAGGGGCCATTTTGGCATT+AGG + Chr1:39337342-39337361 MsG0180002502.01.T01:intron 50.0%
!! GAGGCATTGGACGAAACAAC+AGG + Chr1:39338020-39338039 MsG0180002502.01.T01:CDS 50.0%
AAAGCAGTGCTCCACATCCC+AGG + Chr1:39339063-39339082 MsG0180002502.01.T01:CDS 55.0%
AAGCAGTGCTCCACATCCCA+GGG + Chr1:39339064-39339083 MsG0180002502.01.T01:CDS 55.0%
ACTAAAGATCCCGCATGCGC+AGG + Chr1:39339709-39339728 MsG0180002502.01.T01:intron 55.0%
CCATCGCCCCTCTTGGATAT+GGG - Chr1:39340792-39340811 None:intergenic 55.0%
CGTAAAACGGCTCCAGGTGA+TGG - Chr1:39340717-39340736 None:intergenic 55.0%
CTAAAGATCCCGCATGCGCA+GGG + Chr1:39339710-39339729 MsG0180002502.01.T01:intron 55.0%
GAATAGGAGCTGGGGTCCAT+AGG - Chr1:39340025-39340044 None:intergenic 55.0%
GGAAGTTGTGTCGGATCAGG+TGG + Chr1:39338462-39338481 MsG0180002502.01.T01:CDS 55.0%
GGGGAGAGAAGGGAAGATTG+AGG + Chr1:39337298-39337317 MsG0180002502.01.T01:intron 55.0%
TATTCAGATGCTCCTCGCGG+TGG + Chr1:39340040-39340059 MsG0180002502.01.T01:CDS 55.0%
TGCGGGATCTTTAGTGCACG+GGG - Chr1:39339704-39339723 None:intergenic 55.0%
! GGGGCATCTGAGGATTTGAC+TGG + Chr1:39337319-39337338 MsG0180002502.01.T01:intron 55.0%
! GTCTGAAAGGTGTGCAGCCA+CGG + Chr1:39340175-39340194 MsG0180002502.01.T01:CDS 55.0%
! TCTGAAAGGTGTGCAGCCAC+GGG + Chr1:39340176-39340195 MsG0180002502.01.T01:CDS 55.0%
! TGACTGGGAAGGGGCCATTT+TGG + Chr1:39337335-39337354 MsG0180002502.01.T01:intron 55.0%
!! ACCGCGAGGAGCATCTGAAT+AGG - Chr1:39340041-39340060 None:intergenic 55.0%
!! ACGGCTCCAGGTGATGGATA+AGG - Chr1:39340711-39340730 None:intergenic 55.0%
!! GGAAGATTGAGGGGCATCTG+AGG + Chr1:39337309-39337328 MsG0180002502.01.T01:intron 55.0%
!! GGGCCATTTTGGCATTAGGG+AGG + Chr1:39337346-39337365 MsG0180002502.01.T01:intron 55.0%
!! TTCAGGGTGGTGGGTCTGAA+AGG + Chr1:39340162-39340181 MsG0180002502.01.T01:intron 55.0%
AACCACCACAGCCCATGCCT+TGG - Chr1:39338529-39338548 None:intergenic 60.0%
CATGCGCAGGGTTCAGGAAG+GGG + Chr1:39339722-39339741 MsG0180002502.01.T01:intron 60.0%
CCCATATCCAAGAGGGGCGA+TGG + Chr1:39340789-39340808 MsG0180002502.01.T01:CDS 60.0%
CTTCCTGAACCCTGCGCATG+CGG - Chr1:39339722-39339741 None:intergenic 60.0%
GCATGCGCAGGGTTCAGGAA+GGG + Chr1:39339721-39339740 MsG0180002502.01.T01:intron 60.0%
GCCATCGCCCCTCTTGGATA+TGG - Chr1:39340793-39340812 None:intergenic 60.0%
GCCCGTTAGTAATGCGGGGA+AGG - Chr1:39339865-39339884 None:intergenic 60.0%
TGGAGGTTTCCAGCTAGCCC+TGG - Chr1:39339941-39339960 None:intergenic 60.0%
TTCCTGAACCCTGCGCATGC+GGG - Chr1:39339721-39339740 None:intergenic 60.0%
! AAGGCATGGGCTGTGGTGGT+TGG + Chr1:39338528-39338547 MsG0180002502.01.T01:CDS 60.0%
! AGGCATGGGCTGTGGTGGTT+GGG + Chr1:39338529-39338548 MsG0180002502.01.T01:CDS 60.0%
! TACCAAGGCATGGGCTGTGG+TGG + Chr1:39338524-39338543 MsG0180002502.01.T01:CDS 60.0%
!! CACTAGGGGTGCTAGCTCAC+TGG + Chr1:39339556-39339575 MsG0180002502.01.T01:intron 60.0%
AAGAGGGGCGATGGCAGCGA+TGG + Chr1:39340798-39340817 MsG0180002502.01.T01:CDS 65.0%
AGAGGGGCGATGGCAGCGAT+GGG + Chr1:39340799-39340818 MsG0180002502.01.T01:CDS 65.0%
ATCCCGCATGCGCAGGGTTC+AGG + Chr1:39339716-39339735 MsG0180002502.01.T01:intron 65.0%
ATCGCTGCCATCGCCCCTCT+TGG - Chr1:39340799-39340818 None:intergenic 65.0%
CATCCATACCAGCCACCGCG+AGG - Chr1:39340055-39340074 None:intergenic 65.0%
CGCATGCGCAGGGTTCAGGA+AGG + Chr1:39339720-39339739 MsG0180002502.01.T01:intron 65.0%
GCTGTTGTGTCTGCTGCCCG+TGG - Chr1:39340195-39340214 None:intergenic 65.0%
GGCTGTGGTGGTTGGGGTGT+AGG + Chr1:39338536-39338555 MsG0180002502.01.T01:CDS 65.0%
! GGCATGGGCTGTGGTGGTTG+GGG + Chr1:39338530-39338549 MsG0180002502.01.T01:CDS 65.0%
CAGATGCTCCTCGCGGTGGC+TGG + Chr1:39340044-39340063 MsG0180002502.01.T01:CDS 70.0%
GCTCCTCGCGGTGGCTGGTA+TGG + Chr1:39340049-39340068 MsG0180002502.01.T01:intron 70.0%
! GGCGATGGCAGCGATGGGCT+TGG + Chr1:39340804-39340823 MsG0180002502.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 39337056 39340839 39337056 ID=MsG0180002502.01;
Chr1 mRNA 39337056 39340839 39337056 ID=MsG0180002502.01.T01;Parent=MsG0180002502.01
Chr1 CDS 39337056 39337139 39337056 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39337882 39338041 39337882 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39338398 39338596 39338398 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39339010 39339117 39339010 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39339562 39339577 39339562 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39339989 39340065 39339989 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39340167 39340218 39340167 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Chr1 CDS 39340687 39340839 39340687 ID=MsG0180002502.01.T01.cds;Parent=MsG0180002502.01.T01
Gene Sequence

>MsG0180002502.01.T01

ATGATTCACACATCGCTCTGTCATTATACTTCAAGTTATGGGACCCAGGTGGACACTTATGATATCCTTCACCTTGAGGACAAGGTGGAAGCAAAAGATCCGGGAAGTGGTGCATCTTATTATTATAATGAAAGTACCGGGAAGAGTCAATGGGAAAAACCCCGTGAAGCATCTTTAAATGGGCAATCAACAACGGTTTTGTGTCTTCCAGATAGTTGGGTGGAGGCATTGGACGAAACAACAGGTCACAAATATTACTACAATACAAAAACTAAGGTATCACAATGGAAGCATCCTAATTCTACTCAGGAAGTTGTGTCGGATCAGGTGGTCTCATCAGTTGGTCATCTGGATGACCAATCATCTAATTTACCAAGGCATGGGCTGTGGTGGTTGGGGTGTAGGCCTTGTGCAAGCATGAGTTACTGCAGTCACTGCACAAGAGTTCTCAATCTTCCACAATGTCAGTATTTGTCCTCGAATTTGATTAATCAGCAAAGCAGTGCTCCACATCCCAGGGAAAATTCTGATAAAATGGCTAGCAAACAGAGGGGTGCTAGCTCACTGAAGCGTGCTCATGAAGAAGATGATGAGTTGGATCCTATGGACCCCAGCTCCTATTCAGATGCTCCTCGCGGTGGCTGGGTGGTGGGTCTGAAAGGTGTGCAGCCACGGGCAGCAGACACAACAGCAACCGGTCCTCTGTTTCAACAACGACCTTATCCATCACCTGGAGCCGTTTTACGAAAGAACGCTGAAGTTGCCTCTCAGAAAAAGAAACCAAATTCAAATTTTACTCCCATATCCAAGAGGGGCGATGGCAGCGATGGGCTTGGCGATGCAGATTAG
Protein sequence

>MsG0180002502.01.T01

MIHTSLCHYTSSYGTQVDTYDILHLEDKVEAKDPGSGASYYYNESTGKSQWEKPREASLNGQSTTVLCLPDSWVEALDETTGHKYYYNTKTKVSQWKHPNSTQEVVSDQVVSSVGHLDDQSSNLPRHGLWWLGCRPCASMSYCSHCTRVLNLPQCQYLSSNLINQQSSAPHPRENSDKMASKQRGASSLKRAHEEDDELDPMDPSSYSDAPRGGWVVGLKGVQPRAADTTATGPLFQQRPYPSPGAVLRKNAEVASQKKKPNSNFTPISKRGDGSDGLGDAD*