AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016886.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016886.01.T01 MTR_3g097170 87.121 132 2 1 3 134 72 188 3.26e-66 200
MsG0380016886.01.T01 MTR_3g097170 87.121 132 2 1 3 134 72 188 3.83e-66 200
MsG0380016886.01.T01 MTR_2g007150 59.821 112 30 1 11 122 80 176 4.20e-31 111
MsG0380016886.01.T01 MTR_2g007150 59.821 112 30 1 11 122 121 217 4.65e-31 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016886.01.T01 AT5G13340 65.000 120 27 1 7 126 84 188 5.42e-30 108
MsG0380016886.01.T01 AT1G10890 47.321 112 52 1 12 123 78 182 1.97e-15 70.5
MsG0380016886.01.T01 AT1G10890 47.321 112 52 1 12 123 79 183 2.23e-15 70.5
MsG0380016886.01.T01 AT1G10890 47.321 112 52 1 12 123 126 230 3.00e-15 70.5
MsG0380016886.01.T01 AT1G10890 44.643 112 47 1 12 123 126 222 8.70e-13 63.9

Find 23 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAATTGTGTTAAATTTC+CGG 0.147868 3:-89063481 MsG0380016886.01.T01:CDS
AGATGCTTGAAGAGAATAGA+AGG 0.347191 3:-89062912 MsG0380016886.01.T01:CDS
CTACAATTTATGATGTCGTC+TGG 0.373534 3:-89063936 None:intergenic
AGACGAAGAATTAAAACTAT+TGG 0.380286 3:-89063804 MsG0380016886.01.T01:CDS
GTCTCAGAGAAGAGAAGCTC+TGG 0.388969 3:-89062878 MsG0380016886.01.T01:CDS
CATACAAAATTACCGATTGC+CGG 0.398241 3:+89063462 None:intergenic
TGTGTTAAATTTCCGGCAAT+CGG 0.430948 3:-89063474 MsG0380016886.01.T01:intron
ATGCTCTTACTGAAGCTAGA+AGG 0.450504 3:-89063622 MsG0380016886.01.T01:CDS
TGAAGAGAATAGAAGGAGAG+TGG 0.496275 3:-89062905 MsG0380016886.01.T01:CDS
TGTTGAGGAAAAGCTTAATA+CGG 0.520103 3:-89063738 MsG0380016886.01.T01:CDS
CGAAGAATTAAAACTATTGG+AGG 0.522415 3:-89063801 MsG0380016886.01.T01:CDS
TCGTAAAGAGAGAGAAGAGC+TGG 0.523303 3:-89062938 MsG0380016886.01.T01:CDS
AGCTCTGGAACTGCAGAGGA+AGG 0.525953 3:-89062863 MsG0380016886.01.T01:CDS
TACAATTTATGATGTCGTCT+GGG 0.539257 3:-89063935 None:intergenic
GCTTAATACGGAGGAAGTCA+AGG 0.565124 3:-89063726 MsG0380016886.01.T01:CDS
GGAACTGCAGAGGAAGGAGG+AGG 0.574057 3:-89062857 MsG0380016886.01.T01:CDS
GGAAGGAGGAGGAACGACAA+AGG 0.600196 3:-89062846 MsG0380016886.01.T01:CDS
AAGTCAAGGTGGAAATTGAA+AGG 0.603231 3:-89063712 MsG0380016886.01.T01:CDS
TCTGGAACTGCAGAGGAAGG+AGG 0.605899 3:-89062860 MsG0380016886.01.T01:CDS
ATTGAAAGGCGTGTAGCAGA+AGG 0.606667 3:-89063698 MsG0380016886.01.T01:CDS
GAGAAGCTCTGGAACTGCAG+AGG 0.616097 3:-89062867 MsG0380016886.01.T01:CDS
TGAGGAAAAGCTTAATACGG+AGG 0.690564 3:-89063735 MsG0380016886.01.T01:CDS
TAATACGGAGGAAGTCAAGG+TGG 0.699506 3:-89063723 MsG0380016886.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAATCTTGAATCAAATTT+GGG + Chr3:89063437-89063456 None:intergenic 20.0%
!! ATAGTAATCAAGTCATAAAA+AGG - Chr3:89063557-89063576 MsG0380016886.01.T01:intron 20.0%
!! GATAAAGATGTTTAAAGATA+TGG + Chr3:89063482-89063501 None:intergenic 20.0%
!! GTAGTAATTAGATCATAAAA+AGG - Chr3:89063389-89063408 MsG0380016886.01.T01:intron 20.0%
!! TACAAATCTTGAATCAAATT+TGG + Chr3:89063438-89063457 None:intergenic 20.0%
!! TATCTTTAAACATCTTTATC+TGG - Chr3:89063482-89063501 MsG0380016886.01.T01:CDS 20.0%
!! TCTGTAATTACATTTGAAAA+AGG - Chr3:89063245-89063264 MsG0380016886.01.T01:intron 20.0%
!! TTGAAATTGTGTTAAATTTC+CGG - Chr3:89063283-89063302 MsG0380016886.01.T01:intron 20.0%
!! TTTCAAATGTAATTACAGAA+TGG + Chr3:89063244-89063263 None:intergenic 20.0%
!!! GTTCTTTATCATTTTGTATT+TGG - Chr3:89062919-89062938 MsG0380016886.01.T01:CDS 20.0%
!!! TAATGGATAAAGATAAGATA+TGG + Chr3:89063650-89063669 None:intergenic 20.0%
!!! TGAATACTACTTTTAATGTA+TGG - Chr3:89063758-89063777 MsG0380016886.01.T01:CDS 20.0%
!!! TTAGATCATAAAAAGGTTTT+TGG - Chr3:89063396-89063415 MsG0380016886.01.T01:intron 20.0%
! ACAAATAACAAATCGTACAT+TGG + Chr3:89063727-89063746 None:intergenic 25.0%
! AGACGAAGAATTAAAACTAT+TGG - Chr3:89062960-89062979 MsG0380016886.01.T01:intron 25.0%
AATCAAATTTGGGAATCCTA+TGG + Chr3:89063427-89063446 None:intergenic 30.0%
CGAAGAATTAAAACTATTGG+AGG - Chr3:89062963-89062982 MsG0380016886.01.T01:intron 30.0%
CTAATTACTACTTGTACGAA+CGG + Chr3:89063380-89063399 None:intergenic 30.0%
TAATTACTACTTGTACGAAC+GGG + Chr3:89063379-89063398 None:intergenic 30.0%
TGTTGAGGAAAAGCTTAATA+CGG - Chr3:89063026-89063045 MsG0380016886.01.T01:intron 30.0%
! TAACAAATCGTACATTGGTT+AGG + Chr3:89063722-89063741 None:intergenic 30.0%
!! AAGGTTTTTGGATATTCCAT+AGG - Chr3:89063408-89063427 MsG0380016886.01.T01:intron 30.0%
!! GCTTTTCCTCAACATTTTTT+CGG + Chr3:89063020-89063039 None:intergenic 30.0%
!! GTACATTGGTTAGGTTTTAA+TGG + Chr3:89063713-89063732 None:intergenic 30.0%
AAGTCAAGGTGGAAATTGAA+AGG - Chr3:89063052-89063071 MsG0380016886.01.T01:intron 35.0%
AATCGCCATCTCATAGAAAA+AGG + Chr3:89063200-89063219 None:intergenic 35.0%
AATTACTACTTGTACGAACG+GGG + Chr3:89063378-89063397 None:intergenic 35.0%
AGATGCTTGAAGAGAATAGA+AGG - Chr3:89063852-89063871 MsG0380016886.01.T01:intron 35.0%
AGCTATCCGAAAAAATGTTG+AGG - Chr3:89063011-89063030 MsG0380016886.01.T01:intron 35.0%
ATCGCCATCTCATAGAAAAA+GGG + Chr3:89063199-89063218 None:intergenic 35.0%
CCATTAGCACTATGATAATC+AGG - Chr3:89063664-89063683 MsG0380016886.01.T01:CDS 35.0%
CCTGATTATCATAGTGCTAA+TGG + Chr3:89063667-89063686 None:intergenic 35.0%
TGTGTTAAATTTCCGGCAAT+CGG - Chr3:89063290-89063309 MsG0380016886.01.T01:intron 35.0%
! CATACAAAATTACCGATTGC+CGG + Chr3:89063305-89063324 None:intergenic 35.0%
! GTTACCCTTTTTCTATGAGA+TGG - Chr3:89063192-89063211 MsG0380016886.01.T01:intron 35.0%
! TGTGTTTGTTGAAATTCTGC+AGG - Chr3:89063796-89063815 MsG0380016886.01.T01:CDS 35.0%
!! CATTGGTTAGGTTTTAATGG+TGG + Chr3:89063710-89063729 None:intergenic 35.0%
ATGCTCTTACTGAAGCTAGA+AGG - Chr3:89063142-89063161 MsG0380016886.01.T01:intron 40.0%
CAAAAATCCTGGTTTCTGCA+GGG + Chr3:89063522-89063541 None:intergenic 40.0%
TCTATGAGATGGCGATTGAA+GGG - Chr3:89063203-89063222 MsG0380016886.01.T01:intron 40.0%
TGAAGAGAATAGAAGGAGAG+TGG - Chr3:89063859-89063878 MsG0380016886.01.T01:intron 40.0%
TGAGGAAAAGCTTAATACGG+AGG - Chr3:89063029-89063048 MsG0380016886.01.T01:intron 40.0%
TTCTATGAGATGGCGATTGA+AGG - Chr3:89063202-89063221 MsG0380016886.01.T01:intron 40.0%
! AACCAGGATTTTTGCTCTGT+TGG - Chr3:89063528-89063547 MsG0380016886.01.T01:intron 40.0%
CACCAACAGAGCAAAAATCC+TGG + Chr3:89063533-89063552 None:intergenic 45.0%
CTATGAGATGGCGATTGAAG+GGG - Chr3:89063204-89063223 MsG0380016886.01.T01:intron 45.0%
GCAAAAATCCTGGTTTCTGC+AGG + Chr3:89063523-89063542 None:intergenic 45.0%
GCTTAATACGGAGGAAGTCA+AGG - Chr3:89063038-89063057 MsG0380016886.01.T01:intron 45.0%
TAATACGGAGGAAGTCAAGG+TGG - Chr3:89063041-89063060 MsG0380016886.01.T01:intron 45.0%
TCGTAAAGAGAGAGAAGAGC+TGG - Chr3:89063826-89063845 MsG0380016886.01.T01:intron 45.0%
! ATTGAAAGGCGTGTAGCAGA+AGG - Chr3:89063066-89063085 MsG0380016886.01.T01:intron 45.0%
AAATCCTGGTTTCTGCAGGG+TGG + Chr3:89063519-89063538 None:intergenic 50.0%
AGATTCTGGGTTCTGCAAGG+TGG + Chr3:89063352-89063371 None:intergenic 50.0%
CAGAGATTCTGGGTTCTGCA+AGG + Chr3:89063355-89063374 None:intergenic 50.0%
GTCTCAGAGAAGAGAAGCTC+TGG - Chr3:89063886-89063905 MsG0380016886.01.T01:intron 50.0%
ACGAACGGGGCAGAGATTCT+GGG + Chr3:89063365-89063384 None:intergenic 55.0%
GGAAGGAGGAGGAACGACAA+AGG - Chr3:89063918-89063937 MsG0380016886.01.T01:intron 55.0%
TACGAACGGGGCAGAGATTC+TGG + Chr3:89063366-89063385 None:intergenic 55.0%
! TCTGGAACTGCAGAGGAAGG+AGG - Chr3:89063904-89063923 MsG0380016886.01.T01:intron 55.0%
!! AGCTCTGGAACTGCAGAGGA+AGG - Chr3:89063901-89063920 MsG0380016886.01.T01:intron 55.0%
!! GAGAAGCTCTGGAACTGCAG+AGG - Chr3:89063897-89063916 MsG0380016886.01.T01:intron 55.0%
GGAACTGCAGAGGAAGGAGG+AGG - Chr3:89063907-89063926 MsG0380016886.01.T01:intron 60.0%
TGCGCCACCCTGCAGAAACC+AGG - Chr3:89063512-89063531 MsG0380016886.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 89062840 89063946 89062840 ID=MsG0380016886.01;Name=MsG0380016886.01
Chr3 mRNA 89062840 89063946 89062840 ID=MsG0380016886.01.T01;Parent=MsG0380016886.01;Name=MsG0380016886.01.T01;_AED=0.42;_eAED=0.51;_QI=0|0|0|1|1|1|4|0|135
Chr3 exon 89063936 89063946 89063936 ID=MsG0380016886.01.T01:exon:5693;Parent=MsG0380016886.01.T01
Chr3 exon 89063616 89063838 89063616 ID=MsG0380016886.01.T01:exon:5692;Parent=MsG0380016886.01.T01
Chr3 exon 89063475 89063519 89063475 ID=MsG0380016886.01.T01:exon:5691;Parent=MsG0380016886.01.T01
Chr3 exon 89062840 89062968 89062840 ID=MsG0380016886.01.T01:exon:5690;Parent=MsG0380016886.01.T01
Chr3 CDS 89063936 89063946 89063936 ID=MsG0380016886.01.T01:cds;Parent=MsG0380016886.01.T01
Chr3 CDS 89063616 89063838 89063616 ID=MsG0380016886.01.T01:cds;Parent=MsG0380016886.01.T01
Chr3 CDS 89063475 89063519 89063475 ID=MsG0380016886.01.T01:cds;Parent=MsG0380016886.01.T01
Chr3 CDS 89062840 89062968 89062840 ID=MsG0380016886.01.T01:cds;Parent=MsG0380016886.01.T01
Gene Sequence

>MsG0380016886.01.T01

ATGTCGTCTGGCAAGCGTCAACAAGACGAAGAATTAAAACTATTGGAGGAAGAGACTGCAAGAAGAATAGAAGAAGCTATCCGAAAAAATGTTGAGGAAAAGCTTAATACGGAGGAAGTCAAGGTGGAAATTGAAAGGCGTGTAGCAGAAGGCGTCAAGAAACTGTTTGATGATGTTGAAGTTCAACTTGAAAAAGAAAAGCAAGATGCTCTTACTGAAGCTAGAAGGAAAGAAGTTTCACGTGTCAAAATTGAAATTGTGTTAAATTTCCGGCAATCGGAACAAGCTCGTAAAGAGAGAGAAGAGCTGGATAAGATGCTTGAAGAGAATAGAAGGAGAGTGGAAGAGTCTCAGAGAAGAGAAGCTCTGGAACTGCAGAGGAAGGAGGAGGAACGACAAAGGAATTAG

Protein sequence

>MsG0380016886.01.T01

MSSGKRQQDEELKLLEEETARRIEEAIRKNVEEKLNTEEVKVEIERRVAEGVKKLFDDVEVQLEKEKQDALTEARRKEVSRVKIEIVLNFRQSEQARKEREELDKMLEENRRRVEESQRREALELQRKEEERQRN*