Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002955.01.T01 | XP_013467635.1 | 95.528 | 246 | 6 | 2 | 1 | 246 | 1 | 241 | 2.72E-166 | 470 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002955.01.T01 | A0A072VIM1 | 95.528 | 246 | 6 | 2 | 1 | 246 | 1 | 241 | 1.30e-166 | 470 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0180002955.01 | 0.801501 | 8.742228e-49 | 4.181647e-46 |
| MsG0080047944.01 | MsG0180002955.01 | 0.805937 | 1.049001e-49 | 5.621352e-47 |
| MsG0080047957.01 | MsG0180002955.01 | 0.816365 | 5.740753e-52 | 4.059816e-49 |
| MsG0080047959.01 | MsG0180002955.01 | 0.817872 | 2.632196e-52 | 1.939845e-49 |
| MsG0080048205.01 | MsG0180002955.01 | 0.826228 | 3.043712e-54 | 2.837481e-51 |
| MsG0080048809.01 | MsG0180002955.01 | -0.812035 | 5.192092e-51 | 3.265200e-48 |
| MsG0080048830.01 | MsG0180002955.01 | 0.820797 | 5.673857e-53 | 4.534339e-50 |
| MsG0080048982.01 | MsG0180002955.01 | 0.824285 | 8.766873e-54 | 7.730913e-51 |
| MsG0180000086.01 | MsG0180002955.01 | 0.809701 | 1.661698e-50 | 9.824840e-48 |
| MsG0180000091.01 | MsG0180002955.01 | 0.823186 | 1.586582e-53 | 1.356387e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002955.01.T01 | MTR_1g053570 | 95.528 | 246 | 6 | 2 | 1 | 246 | 1 | 241 | 3.29e-170 | 470 |
Find 71 sgRNAs with CRISPR-Local
Find 247 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAGATGGGTTATACACC+AGG | 0.633040 | 1:+53761014 | MsG0180002955.01.T01:CDS |
| AAAGGCTACCCTTGACCAAT+TGG | 0.446594 | 1:+53761265 | MsG0180002955.01.T01:CDS |
| AAATCACGATCGACAGGAAA+AGG | 0.445020 | 1:+53761153 | MsG0180002955.01.T01:CDS |
| AAGAACGAGGATGATGCTGA+GGG | 0.681423 | 1:+53761314 | MsG0180002955.01.T01:CDS |
| AAGAGCTTTCAATTGGCTCT+TGG | 0.424774 | 1:-53760874 | None:intergenic |
| AAGCTCTTAACTGGCAAGAA+CGG | 0.587734 | 1:+53760889 | MsG0180002955.01.T01:CDS |
| ACAAGAGGATGAACAAACCT+TGG | 0.566502 | 1:+53760941 | MsG0180002955.01.T01:CDS |
| ACGAGCAGGTATAGGCCTAG+AGG | 0.682619 | 1:+53761100 | MsG0180002955.01.T01:CDS |
| ACTACATGGCTGACCTTTCT+AGG | 0.178606 | 1:+53758534 | MsG0180002955.01.T01:CDS |
| AGAAAGAAAGCAAATACAAG+AGG | 0.580568 | 1:+53760926 | MsG0180002955.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGCCACAATTCATTACA+GGG | + | Chr1:53761868-53761887 | MsG0180002955.01.T01:intron | 30.0% | |
| AAACTGCATTTACAAGCTTA+TGG | + | Chr1:53759008-53759027 | MsG0180002955.01.T01:intron | 30.0% | |
| AAGATTAGAGGGGTAAAATT+AGG | + | Chr1:53759179-53759198 | MsG0180002955.01.T01:intron | 30.0% | |
| AAGTTCAAATGCTTAACATG+AGG | - | Chr1:53759809-53759828 | None:intergenic | 30.0% | |
| AATAGCTACATCAGATATCT+TGG | - | Chr1:53759609-53759628 | None:intergenic | 30.0% | |
| AATTGCATTAGAAGACATCA+AGG | - | Chr1:53760613-53760632 | None:intergenic | 30.0% | |
| ACTATTTGCGTATGTATCTT+GGG | - | Chr1:53761526-53761545 | None:intergenic | 30.0% | |
| AGAAAGAAAGCAAATACAAG+AGG | + | Chr1:53760926-53760945 | MsG0180002955.01.T01:CDS | 30.0% | |
| AGCAATACTTAATGCTCTTT+GGG | - | Chr1:53760685-53760704 | None:intergenic | 30.0% | |
| ATAGCTACATCAGATATCTT+GGG | - | Chr1:53759608-53759627 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 53758479 | 53762235 | 53758479 | ID=MsG0180002955.01;Name=MsG0180002955.01 |
| Chr1 | mRNA | 53758479 | 53762235 | 53758479 | ID=MsG0180002955.01.T01;Parent=MsG0180002955.01;Name=MsG0180002955.01.T01;_AED=0.42;_eAED=0.43;_QI=0|0|0|1|1|1|3|0|259 |
| Chr1 | exon | 53758479 | 53758619 | 53758479 | ID=MsG0180002955.01.T01:exon:47746;Parent=MsG0180002955.01.T01 |
| Chr1 | exon | 53760840 | 53761370 | 53760840 | ID=MsG0180002955.01.T01:exon:47747;Parent=MsG0180002955.01.T01 |
| Chr1 | exon | 53762128 | 53762235 | 53762128 | ID=MsG0180002955.01.T01:exon:47748;Parent=MsG0180002955.01.T01 |
| Chr1 | CDS | 53758479 | 53758619 | 53758479 | ID=MsG0180002955.01.T01:cds;Parent=MsG0180002955.01.T01 |
| Chr1 | CDS | 53760840 | 53761370 | 53760840 | ID=MsG0180002955.01.T01:cds;Parent=MsG0180002955.01.T01 |
| Chr1 | CDS | 53762128 | 53762235 | 53762128 | ID=MsG0180002955.01.T01:cds;Parent=MsG0180002955.01.T01 |
| Gene Sequence |
>MsG0180002955.01.T01 ATGATGGCTGAATCAACAACATCTATCAGGCAAAGCGATGATGATGATAACGATGACTACATGGCTGACCTTTCTAGGTTTCTTCCTTCCGATGATGCTATGATGCATCCTTCAATTTCAACTAAATCTTCATCAAAAAGGATTTCTGACAAGAAAGATCCTTCAATCAACTCTTCCAAGAGCCAATTGAAAGCTCTTAACTGGCAAGAACGGCGAAAAATTGAAAGAGAAAGAAAGCAAATACAAGAGGATGAACAAACCTTGGCAAAAGTGGAAGCTCCAATACCACAATCAAACATTGGATTTAAGCTTCTCAAACAGATGGGTTATACACCAGGTTCTGCCCTTGGCAAGCAGGGATCGGGCAGGGCTGAACCAGTGGGTATTGAAATTCGACGGTCACGAGCAGGTATAGGCCTAGAGGATCCCCACAAGGAGAAGAAGAAAAGGGAGGAAATCACGATCGACAGGAAAAGGAAGAATGAGCAGGCTCTGATGGAAGAATTCGGGTCAAGGCAGAAATCGCGATGGCAGAGTAGGAGAGTCATTGTTAATTTTAACAAGGCAAAGGCTACCCTTGACCAATTGGAGAATCGGGAAATTGTGGAACCACCGAAGAACGAGGATGATGCTGAGGGTGAAGATCAAGAAGAGGAAGAAATAACAGAAGAGGACTTGCTCGATGTATTAATGAAACTGAGGGATGAGTTCAATTACTGCCTCTTCTGTGGATGCAAAGTGAGTAAACATGAGTTATCTCTATCGTTTAATATAAACTAA |
| Protein sequence |
>MsG0180002955.01.T01 MMAESTTSIRQSDDDDNDDYMADLSRFLPSDDAMMHPSISTKSSSKRISDKKDPSINSSKSQLKALNWQERRKIERERKQIQEDEQTLAKVEAPIPQSNIGFKLLKQMGYTPGSALGKQGSGRAEPVGIEIRRSRAGIGLEDPHKEKKKREEITIDRKRKNEQALMEEFGSRQKSRWQSRRVIVNFNKAKATLDQLENREIVEPPKNEDDAEGEDQEEEEITEEDLLDVLMKLRDEFNYCLFCGCKVSKHELSLSFNIN* |
